MD03G1219000.v1.1
ERF Family

Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Myosin family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0005645
Physical Location & Seq
Reverse (-)
30341469 .. 30351895
10427 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1219000.v1.1.491

Sequence Viewer

Length: 3462 bp
ATGATGGTTTCCGCGTCACCGTCGATGATAGCGAGGAGCTCATTGGAGGAAATGCTGGATTCGCTTCGGCGGAGAGATGAGGGCGAAAAGCCGAAGGAGTTGCCGCCGGCTTTGCCAGCTCGGCCACCTTCCAGGGCTCGGCTGCCATCAGCCCGCCGCTCTTTGCCCAACAACTTCAGGGTCGAAGGCGCAGAGCGCTCACTGGAGTGTTTGCCTAGTATGAACAAGAGAAAAGACGGGGACTTGGGGTTCAAGACAAGTAATTTTGGCGTGAAGAAGACGGAGAATGATCAAAATGTTGAGTCGCCATACGGCAGGTCACTGAAGGACTGTCGAATGAAGCCGGAGCAGAAGATTGCAAAGTCGGATTGCGATGACAACATTGGTTATTTCATCAAGAAGAAACTTCATGTTTGGTGTCGGCTACCTGGTGGGCTGTGGGAGTTAGGAACGATACAGTCAACTTCAGGGGACGCCGCCCTTGTTTCAGTCTCTAGTGGAAATGTAATCAAAGTTTGCAGAGTTGATCTTTTACCTGCAAACCCAAATGTTCTCGAAGGTGTGGATGATCTTATCCAGCTTAGTTATTTGAATGAGCCTTCTGTACTTTACAATCTTCAATGTAGATATTCCCAGGATATGATTTATAGCAAAGCTGGGCCAGTTTTGATTGCAATCAATCCTTTCAAAGATGTTCAAATTTATGGAGAAGATTTTGTCACAACTTATAGGCGGAAACTAACAGACAAACCTCATGTTTATGCTGTGGCAGACGCTGCTTATGATGAGATGATGGCAGATGATGTAAATCAGTCCATAATCATAAGTGGGGAAAGTGGAGCTGGGAAAACCGAGACAGCAAAAGTTGCAATGCAATACTTGGCTGATCTTGGTAGTGGCAGTTGTGGCACAGAGCATGCAAATGCAATCCTTCAGACCAATTGTATACTAGAAGCGTTTGGAAATGCAAAAACATGCAGAAATCACAATGCTAGTCGATTCGGGAAATTATTTGACATTCATTTTAGCACAGCAGGAAAAATATGTGGTGCTTCTATCCAAACATTTTTTTTGGACAAGTCTAGAGTGACACAACTACAAAATGGTGAGAGGTCGTACCATGTCTTTTATCAACTCTGTGCTGGTGCTCCATCAACGCTAAAAGAGAGGCTAAACCTTAAAAGGGCTAGTGAGTACAAGTATCTTAATCAGAGTGATTGCTTGGAAATTGATGGTGTAGATGATGCGCGGAAGTTTCATATGCTCATGGAAGCGCTAGATGTTGTTCGAGTTTGTAAGGAAGATCAAGAACACGCGTTTTCATTGCTGGCTGCAGTGTTATGGCTGGGAAACATATCATTTCAATCAATTGACAATGAGAATCATGTAGAAGTGTTAGCTGATGAAGCTGTAACCATTGCTGCCATGCTGATGGGTTGTAGTTCTCAGGAACTGATTCTTTCCTTATCCACCCCCGAAATCCATGGTAGCAAGGATAGTATTGTCAAAAGATTGACACTGCGACAGGCAGTTGATGCAAGAGATGCATTGGCAAAATTTATCTATGTCAGCTTGTTTGACTGGCTTGTAGAACAAATTAACAAGTCACTTGCAGTGGGAAAATGCCGTACTGGGAGATCCATCAGTATACTTGATATTCACGGGTTTGAGTCATTCCAGAAGAACAGCTTTGAACAGATGTGCATTAATTATGCAAATGAAAGGCTGCAACAACATTTCAACCGACATTTATTTAAGCTTGAGCAGGAGGAATGTGAATTGGACGGGGTTGATTGGACTAAAGTTGATTTTCAAGACAACCGAGAATGCTTGAATCTGTTTGAGAAGAAACCTTTTGGGCTGCTATCCTTGTTGGATGAGGAATTGAACTTCCCCAAGGCAAATGATTTGACCCTTGCCAATAAATTTAAGCAACACTTGAATGCTAATTCTTGCTTCAAAGCAGAAAAAGCCAGCAGTTTCAGTATTTGTCATCTTGCTGGAGAGGTCCTATATGACACAAGTGGATTCTTGGACAAAAACAGAGATAAGTTGCCCTCTATTTTCTTCCAACTCCTGTCATCGTGCAGTTGCCGACTTCTGCAGTTGTTTACTTCCAAAGAGTTTAAGCAGTTCCAGAAACCAGAAAATTATTCGTGCCAGATGAATGCGTTGGACCCTTCAAAATCAAGCGCTGGTATTGAGTTCAAGGGTCAATTGTTTAAACTAATGCACCAGTTGGAGAGCAGCAAACCTCACTTCATTAGCTGCATAAAGCCAAACAGTAAGCAGCTTCCCGGCATGTATGAAGTAAACCTTGTTTTACAACAGCTCAGGTGTTGTGGAGTTTTGGAGGTTGTTAGGATATCACGATCTGGATATCCTACTAGAATGACACATCAAGAATTTGCTGGAAGGTATGGTTTTCTACTTTTGGAGGACGATTTACCTCAGGATCCATTAAGTCTATCCATTGCTATTCTAAAAAGATTCAGTGTCCTCCCTGAAATGTACCAAATTGGCTATACGAAAGTGTTTCTTAGAACGGGGCAGATTGCATCGTTGGAGGATAAGCGAAAGAAAGTTCTGAGAGGAATAATTGGTGTCCAAAAATACTTCCGTGGTCACCGGGCTCGTTGTCACTTTGATCAACTTAAGGAAGGAGTCGCAAAGATACAATCAAATGGTCTTTGTGAAAATACTGGAAGGAAACCAGGTGCTTGTGAAACTGTTGATGAGCGGCAAAATTTGAAGAGGTCACATCCTGAGAATGGGAAAGCTAAAAGGAAGGCCGGCAGGAAGATGTCAGAGGTGAAGGATTTGCCATCAGATTTGGCAGAGCTTCAAAGGCGGGTGCTTCAGGCTGAAGCAACTCTGGTGCGGAAGGAGGAGGAAAATGCTGAATTACAGGAGCAACTGCAACAATTTGAAACAAGATGGTCAGAATATGAATCAAAGATGAAATCGATGCAGGACGTGTGGCAGAAGCAGATGGTGTCTTTACAGACGAGTCTTGCTGCGGCCAGAAAGAGTCTTGGATCTGCCAATACTGCTGGTCAACCTGTAACACTAGGTTCTGTTTCATCGGCCCGCTATGATTCTGAAGAAGCCACATCTATGGGATCTCGAACTCCTGGTGCAAGCACACCCCTCAATGGAGCAGGGCGTGAGGCTAATGGCAGCTCGAATGCAGTCAGCAATCTGTTGAAGGAATTTGAGCAGCGGAGACAGATATTCGATGATGATGCGAAAGCTCTGGTTGAGGTAAAACCTGGGCAGTCAGCTGCAAATATGAATCCTGAGGAAGATTTACGAAAACTTAAACACCGGTTTGAGTCCTGGAAGAAAGAGTACAAGGCAAGATTACGGGAGACAAAGACAAAGCTTCACAAAATTTGGCACTCAGAAGAGGAAAAACGGCGGAGAAAATGGTGGGGAAAGATAGGCTCAAGAGCATTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000146 GO:0000166 GO:0000278 GO:0000281 GO:0000302 GO:0000910 GO:0001891 GO:0001931 GO:0003674 GO:0003774 GO:0003779 GO:0003824 GO:0005488 GO:0005515 GO:0005524 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005768 GO:0005826 GO:0005829 GO:0005856 GO:0005886 GO:0005911 GO:0005938 GO:0006928 GO:0006935 GO:0006950 GO:0006970 GO:0006971 GO:0006979 GO:0006996 GO:0007010 GO:0007015 GO:0007033 GO:0007049 GO:0007154 GO:0008064 GO:0008092 GO:0008104 GO:0008144 GO:0008150 GO:0008360 GO:0009267 GO:0009506 GO:0009581 GO:0009582 GO:0009605 GO:0009612 GO:0009628 GO:0009636 GO:0009987 GO:0009991 GO:0010033 GO:0010035 GO:0010243 GO:0010639 GO:0012505 GO:0014070 GO:0014074 GO:0015629 GO:0016020 GO:0016043 GO:0016050 GO:0016459 GO:0016460 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017076 GO:0017111 GO:0022402 GO:0022603 GO:0022604 GO:0022607 GO:0030029 GO:0030030 GO:0030031 GO:0030036 GO:0030038 GO:0030048 GO:0030054 GO:0030139 GO:0030554 GO:0030587 GO:0030832 GO:0030833 GO:0030837 GO:0030863 GO:0030864 GO:0030865 GO:0030866 GO:0030898 GO:0031033 GO:0031034 GO:0031152 GO:0031154 GO:0031254 GO:0031268 GO:0031270 GO:0031333 GO:0031410 GO:0031667 GO:0031668 GO:0031669 GO:0031982 GO:0032009 GO:0032060 GO:0032153 GO:0032155 GO:0032271 GO:0032272 GO:0032502 GO:0032535 GO:0032553 GO:0032555 GO:0032559 GO:0032796 GO:0032956 GO:0032970 GO:0032982 GO:0032991 GO:0033036 GO:0033043 GO:0033275 GO:0033298 GO:0033554 GO:0034461 GO:0034622 GO:0035639 GO:0036094 GO:0040011 GO:0042221 GO:0042330 GO:0042493 GO:0042542 GO:0042594 GO:0042623 GO:0042641 GO:0042802 GO:0042803 GO:0042995 GO:0043167 GO:0043168 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043232 GO:0043254 GO:0043933 GO:0044085 GO:0044087 GO:0044422 GO:0044424 GO:0044425 GO:0044430 GO:0044444 GO:0044446 GO:0044448 GO:0044459 GO:0044464 GO:0044764 GO:0044877 GO:0045177 GO:0045179 GO:0045335 GO:0046677 GO:0046683 GO:0046847 GO:0046983 GO:0048519 GO:0048523 GO:0048856 GO:0048870 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0050982 GO:0051015 GO:0051017 GO:0051128 GO:0051129 GO:0051179 GO:0051301 GO:0051493 GO:0051494 GO:0051591 GO:0051606 GO:0051674 GO:0051703 GO:0051704 GO:0051716 GO:0055044 GO:0060327 GO:0060328 GO:0061572 GO:0061640 GO:0065003 GO:0065007 GO:0065008 GO:0070252 GO:0070938 GO:0071496 GO:0071840 GO:0071889 GO:0071944 GO:0090066 GO:0090702 GO:0097159 GO:0097204 GO:0097367 GO:0097435 GO:0097708 GO:0098630 GO:0098743 GO:0099120 GO:0099568 GO:0099738 GO:0110053 GO:0120025 GO:0120031 GO:0120036 GO:1901265 GO:1901363 GO:1901698 GO:1901700 GO:1902903 GO:1902904 GO:1903047 GO:1990753
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
IQ Myosin_N Myosin_head

Protein Analysis

1154

Amino Acids

130.07

Weight (kDa)

8.65

Isoelectric Point (pI)

48.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SH3_VIII-1_N PF25369 136 - 180 1.8e-19 Myosin-1-like, N-terminal SH3 domain
Myosin_head PF00063 188 - 848 4.3e-224 Myosin head (motor domain)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 306, 544
AccBSI CCGCTC 2 cut(s) 159, 2740
AccI GTMKAC 2 cut(s) 946, 1648
AccII CGCG 3 cut(s) 14, 1249, 1316
AclWI GGATC 5 cut(s) 1632, 2450, 2463, 3046, 3130
AcoI YGGCCR 2 cut(s) 122, 3021
AcsI RAATTY 7 cut(s) 699, 1556, 1925, 2405, 2746, 3212, 3393
AcuI CTGAAG 7 cut(s) 160, 344, 450, 917, 2843, 2886, 3123
AcyI GRCGYC 1 cut(s) 474
AfaI GTAC 6 cut(s) 606, 1118, 1196, 1630, 2513, 3353
AfeI AGCGCT 3 cut(s) 197, 1275, 2194
AfiI CCNNNNNNNGG 4 cut(s) 138, 1183, 2771, 3155
AflII CTTAAG 1 cut(s) 2654
AflIII ACRYGT 2 cut(s) 1314, 2976
AgeI ACCGGT 1 cut(s) 3327
AjiI CACGTC 1 cut(s) 2977
AjnI CCWGG 7 cut(s) 131, 427, 633, 2713, 3133, 3271, 3338
AleI CACNNNNGTG 1 cut(s) 205
AloI GAACNNNNNNTCC 2 cut(s) 233, 265
Alw21I GWGCWC 2 cut(s) 41, 1150
Alw26I GTCTC 4 cut(s) 496, 848, 3220, 3365
AlwI GGATC 5 cut(s) 1632, 2450, 2463, 3046, 3130
AlwNI CAGNNNCTG 2 cut(s) 776, 1453
Aor51HI AGCGCT 3 cut(s) 197, 1275, 2194
AoxI GGCC 5 cut(s) 122, 659, 2790, 3021, 3087
ApoI RAATTY 7 cut(s) 699, 1556, 1925, 2405, 2746, 3212, 3393
AseI ATTAAT 1 cut(s) 1707
AsiGI ACCGGT 1 cut(s) 3327
Asp700I GAANNNNTTC 1 cut(s) 1455
AspLEI GCGC 5 cut(s) 191, 198, 1249, 1276, 2195
AspS9I GGNCC 4 cut(s) 659, 2008, 2176, 3088
AsuC2I CCSGG 2 cut(s) 2298, 2630
AsuHPI GGTGA 4 cut(s) 9, 1118, 2618, 2824
AvaII GGWCC 2 cut(s) 2008, 2176
AxyI CCTNAGG 2 cut(s) 2451, 3300
BamHI GGATCC 1 cut(s) 2455
BanII GRGCYC 3 cut(s) 41, 139, 2635
BarI GAAGNNNNNNTAC 2 cut(s) 3335, 3367
BbsI GAAGAC 1 cut(s) 284
Bbv12I GWGCWC 2 cut(s) 41, 1150
BccI CCATC 9 cut(s) 154, 787, 1159, 1226, 1426, 1649, 2833, 2931, 2986
BceAI ACGGC 3 cut(s) 328, 1611, 3434
BcgI CGANNNNNNTGC 4 cut(s) 82, 116, 3227, 3261
BciT130I CCWGG 7 cut(s) 133, 429, 635, 2715, 3135, 3273, 3340
BclI TGATCA 2 cut(s) 289, 2647
BcnI CCSGG 2 cut(s) 2298, 2630
BcoDI GTCTC 4 cut(s) 496, 848, 3220, 3365
BfaI CTAG 9 cut(s) 216, 495, 950, 993, 1083, 1188, 1277, 2388, 3071
BfmI CTRYAG 2 cut(s) 1332, 2102
BfoI RGCGCY 3 cut(s) 199, 1277, 2196
BfrI CTTAAG 1 cut(s) 2654
BfuAI ACCTGC 2 cut(s) 306, 544
BglI GCCNNNNNGGC 1 cut(s) 121
Bme1390I CCNGG 9 cut(s) 133, 429, 635, 2298, 2630, 2715, 3135, 3273, 3340
Bme18I GGWCC 2 cut(s) 2008, 2176
BmgBI CACGTC 1 cut(s) 2977
BmgT120I GGNCC 4 cut(s) 659, 2008, 2176, 3088
BmiI GGNNCC 2 cut(s) 2178, 2457
BmrFI CCNGG 9 cut(s) 133, 429, 635, 2298, 2630, 2715, 3135, 3273, 3340
BmrI ACTGGG 1 cut(s) 1641
BmsI GCATC 6 cut(s) 1234, 1525, 1534, 2567, 2958, 3235
BmuI ACTGGG 1 cut(s) 1641
BpiI GAAGAC 1 cut(s) 284
BpmI CTGGAG 2 cut(s) 224, 2022
Bpu10I CCTNAGC 1 cut(s) 2333
BpuEI CTTGAG 2 cut(s) 1781, 3433
BpuMI CCSGG 2 cut(s) 2298, 2630
Bsa29I ATCGAT 1 cut(s) 2966
BsaBI GATNNNNATC 2 cut(s) 674, 807
BsaHI GRCGYC 1 cut(s) 474
BsaJI CCNNGG 6 cut(s) 132, 633, 1483, 1896, 2620, 3272
BsaWI WCCGGW 1 cut(s) 3327
Bsc4I CCNNNNNNNGG 4 cut(s) 138, 1183, 2771, 3155
Bse118I RCCGGY 3 cut(s) 106, 2792, 3327
Bse1I ACTGG 6 cut(s) 207, 662, 1586, 1636, 2236, 2707
Bse21I CCTNAGG 2 cut(s) 2451, 3300
Bse3DI GCAATG 4 cut(s) 876, 1322, 1416, 2472
Bse8I GATNNNNATC 2 cut(s) 674, 807
BseBI CCWGG 7 cut(s) 133, 429, 635, 2715, 3135, 3273, 3340
BseCI ATCGAT 1 cut(s) 2966
BseDI CCNNGG 6 cut(s) 132, 633, 1483, 1896, 2620, 3272
BseGI GGATG 3 cut(s) 571, 1882, 2761
BseJI GATNNNNATC 2 cut(s) 674, 807
BseLI CCNNNNNNNGG 4 cut(s) 138, 1183, 2771, 3155
BseMI GCAATG 4 cut(s) 876, 1322, 1416, 2472
BseMII CTCAG 7 cut(s) 1460, 2347, 2465, 2579, 2757, 3291, 3417
BseNI ACTGG 6 cut(s) 207, 662, 1586, 1636, 2236, 2707
BseRI GAGGAG 2 cut(s) 49, 2903
BseYI CCCAGC 3 cut(s) 656, 842, 1345
BsgI GTGCAG 1 cut(s) 2107
Bsh1236I CGCG 3 cut(s) 14, 1249, 1316
BshFI GGCC 5 cut(s) 124, 661, 2792, 3023, 3089
BshTI ACCGGT 1 cut(s) 3327
BshVI ATCGAT 1 cut(s) 2966
BsiHKAI GWGCWC 2 cut(s) 41, 1150
BsiSI CCGG 6 cut(s) 107, 344, 2298, 2629, 2793, 3328
BslFI GGGAC 2 cut(s) 254, 485
BslI CCNNNNNNNGG 4 cut(s) 138, 1183, 2771, 3155
BsmAI GTCTC 4 cut(s) 496, 848, 3220, 3365
BsmFI GGGAC 2 cut(s) 254, 485
BsmI GAATGC 4 cut(s) 1832, 1948, 2173, 3193
BsnI GGCC 5 cut(s) 124, 661, 2792, 3023, 3089
Bsp1286I GDGCHC 4 cut(s) 41, 139, 1150, 2635
Bsp19I CCATGG 1 cut(s) 1483
BspANI GGCC 5 cut(s) 124, 661, 2792, 3023, 3089
BspCNI CTCAG 7 cut(s) 1459, 2346, 2464, 2580, 2758, 3292, 3416
BspDI ATCGAT 1 cut(s) 2966
BspFNI CGCG 3 cut(s) 14, 1249, 1316
BspLI GGNNCC 2 cut(s) 2178, 2457
BspMAI CTGCAG 2 cut(s) 1336, 2106
BspMI ACCTGC 2 cut(s) 306, 544
BspPI GGATC 5 cut(s) 1632, 2450, 2463, 3046, 3130
BspTI CTTAAG 1 cut(s) 2654
BsrBI CCGCTC 2 cut(s) 159, 2740
BsrDI GCAATG 4 cut(s) 876, 1322, 1416, 2472
BsrFI RCCGGY 3 cut(s) 106, 2792, 3327
BsrI ACTGG 6 cut(s) 207, 662, 1586, 1636, 2236, 2707
BssAI RCCGGY 3 cut(s) 106, 2792, 3327
BssECI CCNNGG 6 cut(s) 132, 633, 1483, 1896, 2620, 3272
BssNAI GTATAC 2 cut(s) 947, 1649
BssNI GRCGYC 1 cut(s) 474
BssT1I CCWWGG 2 cut(s) 1483, 1896
Bst1107I GTATAC 2 cut(s) 947, 1649
Bst2UI CCWGG 7 cut(s) 133, 429, 635, 2715, 3135, 3273, 3340
Bst4CI ACNGT 5 cut(s) 21, 332, 459, 2285, 2731
Bst6I CTCTTC 2 cut(s) 2747, 3402
BstACI GRCGYC 1 cut(s) 474
BstAFI CTTAAG 1 cut(s) 2654
BstAPI GCANNNNNTGC 4 cut(s) 776, 866, 1535, 1544
BstC8I GCNNGC 9 cut(s) 108, 117, 154, 918, 1329, 1975, 2794, 3091, 3142
BstDEI CTNAG 9 cut(s) 581, 1446, 2333, 2451, 2540, 2588, 2766, 3300, 3403
BstDSI CCRYGG 2 cut(s) 1483, 2620
BstEII GGTNACC 1 cut(s) 2624
BstF5I GGATG 3 cut(s) 571, 1882, 2761
BstFNI CGCG 3 cut(s) 14, 1249, 1316
BstH2I RGCGCY 3 cut(s) 199, 1277, 2196
BstHHI GCGC 5 cut(s) 191, 198, 1249, 1276, 2195
BstMAI GTCTC 4 cut(s) 496, 848, 3220, 3365
BstNI CCWGG 7 cut(s) 133, 429, 635, 2715, 3135, 3273, 3340
BstNSI RCATGY 3 cut(s) 920, 978, 2305
BstPI GGTNACC 1 cut(s) 2624
BstSCI CCNGG 9 cut(s) 131, 427, 633, 2296, 2628, 2713, 3133, 3271, 3338
BstSFI CTRYAG 2 cut(s) 1332, 2102
BstUI CGCG 3 cut(s) 14, 1249, 1316
BstV2I GAAGAC 1 cut(s) 284
BstX2I RGATCY 4 cut(s) 1637, 2455, 3038, 3122
BstXI CCANNNNNNTGG 2 cut(s) 1432, 3118
BstYI RGATCY 4 cut(s) 1637, 2455, 3038, 3122
BstZ17I GTATAC 2 cut(s) 947, 1649
Bsu15I ATCGAT 1 cut(s) 2966
Bsu36I CCTNAGG 2 cut(s) 2451, 3300
BsuRI GGCC 5 cut(s) 124, 661, 2792, 3023, 3089
BsuTUI ATCGAT 1 cut(s) 2966
BtgI CCRYGG 2 cut(s) 1483, 2620
BtgZI GCGATG 1 cut(s) 387
BtrI CACGTC 1 cut(s) 2977
BtsCI GGATG 3 cut(s) 571, 1882, 2761
BtsI GCAGTG 3 cut(s) 1341, 1517, 1620
BtsIMutI CAGTG 6 cut(s) 200, 320, 1341, 1517, 1620, 2500
BveI ACCTGC 2 cut(s) 306, 544
Cac8I GCNNGC 9 cut(s) 108, 117, 154, 918, 1329, 1975, 2794, 3091, 3142
CaiI CAGNNNCTG 2 cut(s) 776, 1453
CfoI GCGC 5 cut(s) 191, 198, 1249, 1276, 2195
Cfr10I RCCGGY 3 cut(s) 106, 2792, 3327
Cfr13I GGNCC 4 cut(s) 659, 2008, 2176, 3088
ClaI ATCGAT 1 cut(s) 2966
CseI GACGC 3 cut(s) 3, 482, 782
CsiI ACCWGGT 2 cut(s) 427, 2713
Csp6I GTAC 6 cut(s) 605, 1117, 1195, 1629, 2512, 3352
CspAI ACCGGT 1 cut(s) 3327
CviQI GTAC 6 cut(s) 605, 1117, 1195, 1629, 2512, 3352
DdeI CTNAG 9 cut(s) 581, 1446, 2333, 2451, 2540, 2588, 2766, 3300, 3403
DraI TTTAAA 1 cut(s) 2224
EaeI YGGCCR 2 cut(s) 122, 3021
Eam1104I CTCTTC 2 cut(s) 2747, 3402
EarI CTCTTC 2 cut(s) 2747, 3402
EciI GGCGGA 3 cut(s) 85, 748, 3436
Ecl136II GAGCTC 1 cut(s) 39
Eco130I CCWWGG 2 cut(s) 1483, 1896
Eco24I GRGCYC 3 cut(s) 41, 139, 2635
Eco32I GATATC 2 cut(s) 2367, 2381
Eco47I GGWCC 2 cut(s) 2008, 2176
Eco47III AGCGCT 3 cut(s) 197, 1275, 2194
Eco53kI GAGCTC 1 cut(s) 39
Eco57I CTGAAG 7 cut(s) 160, 344, 450, 917, 2843, 2886, 3123
Eco81I CCTNAGG 2 cut(s) 2451, 3300
Eco91I GGTNACC 1 cut(s) 2624
EcoICRI GAGCTC 1 cut(s) 39
EcoO109I RGGNCCY 1 cut(s) 2008
EcoO65I GGTNACC 1 cut(s) 2624
EcoRII CCWGG 7 cut(s) 131, 427, 633, 2713, 3133, 3271, 3338
EcoRV GATATC 2 cut(s) 2367, 2381
EcoT14I CCWWGG 2 cut(s) 1483, 1896
EcoT22I ATGCAT 1 cut(s) 1549
EcoT38I GRGCYC 3 cut(s) 41, 139, 2635
ErhI CCWWGG 2 cut(s) 1483, 1896
FalI AAGNNNNNCTT 8 cut(s) 1673, 1705, 1922, 1954, 2301, 2333, 2523, 2555
FaqI GGGAC 2 cut(s) 254, 485
FauI CCCGC 3 cut(s) 161, 2844, 3098
FauNDI CATATG 1 cut(s) 1260
FbaI TGATCA 2 cut(s) 289, 2647
FblI GTMKAC 2 cut(s) 946, 1648
FokI GGATG 3 cut(s) 578, 1889, 2748
FriOI GRGCYC 3 cut(s) 41, 139, 2635
FspBI CTAG 9 cut(s) 216, 495, 950, 993, 1083, 1188, 1277, 2388, 3071
GlaI GCGC 5 cut(s) 190, 197, 1248, 1275, 2194
GsaI CCCAGC 3 cut(s) 660, 846, 1349
GsuI CTGGAG 2 cut(s) 224, 2022
HaeII RGCGCY 3 cut(s) 199, 1277, 2196
HaeIII GGCC 5 cut(s) 124, 661, 2792, 3023, 3089
HapII CCGG 6 cut(s) 107, 344, 2298, 2629, 2793, 3328
HgaI GACGC 3 cut(s) 3, 482, 782
HhaI GCGC 5 cut(s) 191, 198, 1249, 1276, 2195
Hin1I GRCGYC 1 cut(s) 474
Hin6I GCGC 5 cut(s) 189, 196, 1247, 1274, 2193
HinP1I GCGC 5 cut(s) 189, 196, 1247, 1274, 2193
HincII GTYRAC 2 cut(s) 462, 3059
HindII GTYRAC 2 cut(s) 462, 3059
HindIII AAGCTT 2 cut(s) 1757, 3383
HpaII CCGG 6 cut(s) 107, 344, 2298, 2629, 2793, 3328
HphI GGTGA 4 cut(s) 9, 1118, 2618, 2824
Hpy166II GTNNAC 6 cut(s) 462, 947, 1649, 2112, 2314, 3059
Hpy188I TCNGA 9 cut(s) 367, 936, 1212, 2589, 2809, 2830, 2944, 3103, 3406
Hpy8I GTNNAC 6 cut(s) 462, 947, 1649, 2112, 2314, 3059
Hpy99I CGWCG 1 cut(s) 25
HpyCH4III ACNGT 5 cut(s) 21, 332, 459, 2285, 2731
HpyCH4IV ACGT 1 cut(s) 2976
HpyF3I CTNAG 9 cut(s) 581, 1446, 2333, 2451, 2540, 2588, 2766, 3300, 3403
HpySE526I ACGT 1 cut(s) 2976
Hsp92I GRCGYC 1 cut(s) 474
HspAI GCGC 5 cut(s) 189, 196, 1247, 1274, 2193
KroI GCCGGC 2 cut(s) 106, 2792
KroNI GCCGGC 2 cut(s) 108, 2794
Ksp22I TGATCA 2 cut(s) 289, 2647
LmnI GCTCC 6 cut(s) 36, 346, 839, 1153, 2911, 3158
LweI GCATC 6 cut(s) 1234, 1525, 1534, 2567, 2958, 3235
MabI ACCWGGT 2 cut(s) 427, 2713
MaeI CTAG 9 cut(s) 216, 495, 950, 993, 1083, 1188, 1277, 2388, 3071
MaeII ACGT 1 cut(s) 2976
MbiI CCGCTC 2 cut(s) 159, 2740
MfeI CAATTG 3 cut(s) 940, 1368, 2216
MflI RGATCY 4 cut(s) 1637, 2455, 3038, 3122
MhlI GDGCHC 4 cut(s) 41, 139, 1150, 2635
MluI ACGCGT 1 cut(s) 1314
MlyI GAGTC 6 cut(s) 311, 1679, 2673, 3019, 3040, 3344
MmeI TCCRAC 6 cut(s) 345, 1854, 2095, 2154, 2220, 2544
Mph1103I ATGCAT 1 cut(s) 1549
MroNI GCCGGC 2 cut(s) 106, 2792
MroXI GAANNNNTTC 1 cut(s) 1455
MslI CAYNNNNRTG 6 cut(s) 205, 759, 921, 1430, 1941, 3116
MspA1I CMGCKG 2 cut(s) 3223, 3284
MspCI CTTAAG 1 cut(s) 2654
MspI CCGG 6 cut(s) 107, 344, 2298, 2629, 2793, 3328
MspR9I CCNGG 9 cut(s) 133, 429, 635, 2298, 2630, 2715, 3135, 3273, 3340
MssI GTTTAAAC 1 cut(s) 2224
MunI CAATTG 3 cut(s) 940, 1368, 2216
Mva1269I GAATGC 4 cut(s) 1832, 1948, 2173, 3193
MvaI CCWGG 7 cut(s) 133, 429, 635, 2715, 3135, 3273, 3340
MvnI CGCG 3 cut(s) 14, 1249, 1316
NaeI GCCGGC 2 cut(s) 108, 2794
NciI CCSGG 2 cut(s) 2298, 2630
NcoI CCATGG 1 cut(s) 1483
NdeI CATATG 1 cut(s) 1260
NgoMIV GCCGGC 2 cut(s) 106, 2792
NlaIV GGNNCC 2 cut(s) 2178, 2457
NmeAIII GCCGAG 2 cut(s) 100, 118
NmuCI GTSAC 8 cut(s) 15, 318, 718, 1087, 1605, 2624, 2639, 2757
NsiI ATGCAT 1 cut(s) 1549
NspI RCATGY 3 cut(s) 920, 978, 2305
OliI CACNNNNGTG 1 cut(s) 205
PaeI GCATGC 1 cut(s) 920
PcsI WCGNNNNNNNCGW 1 cut(s) 29
PctI GAATGC 4 cut(s) 1832, 1948, 2173, 3193
PdiI GCCGGC 2 cut(s) 108, 2794
PdmI GAANNNNTTC 1 cut(s) 1455
PfoI TCCNGGA 1 cut(s) 3338
PinAI ACCGGT 1 cut(s) 3327
PleI GAGTC 6 cut(s) 310, 1678, 2672, 3018, 3039, 3343
PmeI GTTTAAAC 1 cut(s) 2224
PpsI GAGTC 6 cut(s) 310, 1678, 2672, 3018, 3039, 3343
PpuMI RGGWCCY 1 cut(s) 2008
PshBI ATTAAT 1 cut(s) 1707
Psp124BI GAGCTC 1 cut(s) 41
Psp5II RGGWCCY 1 cut(s) 2008
Psp6I CCWGG 7 cut(s) 131, 427, 633, 2713, 3133, 3271, 3338
PspEI GGTNACC 1 cut(s) 2624
PspFI CCCAGC 3 cut(s) 656, 842, 1345
PspGI CCWGG 7 cut(s) 131, 427, 633, 2713, 3133, 3271, 3338
PspN4I GGNNCC 2 cut(s) 2178, 2457
PspPI GGNCC 4 cut(s) 659, 2008, 2176, 3088
PspPPI RGGWCCY 1 cut(s) 2008
PstI CTGCAG 2 cut(s) 1336, 2106
PstNI CAGNNNCTG 2 cut(s) 776, 1453
PsuI RGATCY 4 cut(s) 1637, 2455, 3038, 3122
PvuII CAGCTG 1 cut(s) 3284
RsaI GTAC 6 cut(s) 606, 1118, 1196, 1630, 2513, 3353
RsaNI GTAC 6 cut(s) 605, 1117, 1195, 1629, 2512, 3352
RseI CAYNNNNRTG 6 cut(s) 205, 759, 921, 1430, 1941, 3116
SacI GAGCTC 1 cut(s) 41
Sau96I GGNCC 4 cut(s) 659, 2008, 2176, 3088
SchI GAGTC 6 cut(s) 311, 1679, 2673, 3019, 3040, 3344
ScrFI CCNGG 9 cut(s) 133, 429, 635, 2298, 2630, 2715, 3135, 3273, 3340
SduI GDGCHC 4 cut(s) 41, 139, 1150, 2635
SexAI ACCWGGT 2 cut(s) 427, 2713
SfaNI GCATC 6 cut(s) 1234, 1525, 1534, 2567, 2958, 3235
SfcI CTRYAG 2 cut(s) 1332, 2102
SinI GGWCC 2 cut(s) 2008, 2176
SmiMI CAYNNNNRTG 6 cut(s) 205, 759, 921, 1430, 1941, 3116
SmlI CTYRAG 3 cut(s) 1760, 2654, 3448
SmoI CTYRAG 3 cut(s) 1760, 2654, 3448
SphI GCATGC 1 cut(s) 920
SspMI CTAG 9 cut(s) 216, 495, 950, 993, 1083, 1188, 1277, 2388, 3071
SstI GAGCTC 1 cut(s) 41
StyD4I CCNGG 9 cut(s) 131, 427, 633, 2296, 2628, 2713, 3133, 3271, 3338
StyI CCWWGG 2 cut(s) 1483, 1896
TaaI ACNGT 5 cut(s) 21, 332, 459, 2285, 2731
TaiI ACGT 1 cut(s) 2979
TatI WGTACW 3 cut(s) 604, 1194, 3351
TauI GCSGC 5 cut(s) 106, 159, 479, 2743, 3023
TscAI CASTG 6 cut(s) 207, 327, 1341, 1524, 1620, 2500
TseFI GTSAC 8 cut(s) 15, 318, 718, 1087, 1605, 2624, 2639, 2757
Tsp45I GTSAC 8 cut(s) 15, 318, 718, 1087, 1605, 2624, 2639, 2757
TspGWI ACGGA 2 cut(s) 296, 2609
TspRI CASTG 6 cut(s) 207, 327, 1341, 1524, 1620, 2500
Vha464I CTTAAG 1 cut(s) 2654
VpaK11BI GGWCC 2 cut(s) 2008, 2176
VspI ATTAAT 1 cut(s) 1707
XapI RAATTY 7 cut(s) 699, 1556, 1925, 2405, 2746, 3212, 3393
XbaI TCTAGA 1 cut(s) 1082
XceI RCATGY 3 cut(s) 920, 978, 2305
XmiI GTMKAC 2 cut(s) 946, 1648
XmnI GAANNNNTTC 1 cut(s) 1455
XspI CTAG 9 cut(s) 216, 495, 950, 993, 1083, 1188, 1277, 2388, 3071
Zsp2I ATGCAT 1 cut(s) 1549
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.