pycom11g20840
ERF Family

Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Myosin family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr11
OG0005645
Physical Location & Seq
Reverse (-)
23992320 .. 24001152
8833 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom11g20840.5

Sequence Viewer

Length: 2811 bp
ATGTTCTCGAAGGTGTGGATCGATCTTATACAGCTTAGTTATTTGAATGAGCCTGCTGTTCTTTACAATCTTCAGTGTAGATATTCCCAGGATATGATTTATAGCAAAGCCGGGCCAGTTTTGATTGCAATCAATCCTTTCAAAGATGTTCAAATTTATGGAAAAGATTCTATTAGAGCTTATAGGCAGAAACTAACAGACAAACCTCATGTCTATGCTGTGGCAGACGCTGCTTATGACGAGATGATGACAGCTGGGAAAACCGAGACAGCAAAAGTTGCAATGCAATACTTGGCTGCTCTTGGTAGTGGCAGTTGTGGCACAGAGCATGCAAATGCAATCCTTCAGACCAATTGTATACTAGAAGCGTTTGGAAATGCAAAAACATCCAGAAATCAAAATGCTAGCCGATTCAGGCTAAACCTTAAAAGGGCTAGTGAGTACAAGTATCTTAATCAGAGTGATTGTTTGGAAATCGATGGTGTAGATGATGCGCGAAAGTTTCATATACTTGTGGAAGCGCTAGATGTTGTTCATGTTTGTAAGGAAGATCAAGAACACATGTTTTCATTGCTGGCCGCAGTGTTATGGCTGGGAAACATATCATTTCGAGCGATTGACAATGAGAAGCATGTGGAAGTGTTAGCTGATGAAGCTGTAACCATTGCTTCCATGCTGATGGGTTGTAGTTCCCAGGAGCTAATTCTTTCTTTGTCCACCCCCGAAATCCATGGTGCCAAGGATAGTATTGACACAAGGCTAACACTGAGACAGGCAATTGATGCAAGAGATGCCTTGGCAAAATTTATCTATGTTAGCTTGTTTGACTGGCTTGTAGAACAAATTAATAAGTCACTTGCAGTGGGAAAATGCCGTACTGGGAGATCCATCAGTATACTTGATATTTATGGGTTTGAGTCATTCCAGAAGAACAGCTTTGAACAAATGTGCATTAATTATGCAAATGAGAGGCTGCAACAACATTTCAACCGACATTTATTTAAGCTTGAGCAGGAGGAATGTGAATTGGATGGGCTTGATTGGACTAAAGTTGATTTTCAAGACAATCAAGAATGCTTGAATCTGTTTGAGAAGAAACCTTTTGGGCTACTATCCATGTTGGATGAGGAATCGAACTTCCCTAAGGCAAATGATTTGACCCTTGCCAATAAATTTAAGCAACACTTGAATGCTAATTCTTGCTTTAAAGAAGAAAAAGGAAGCACTTTCAGTATTCGTCATCATGCTGGAGAGGTTCTATATGACACAAGTGGCTTCCTGAAAAAAAACAGAGATAGGTTGCCTTCTGTTTTCTTCCAACTCCTGTCATCGTGCAGATGCCGGCTTCTGCAGTTGTTTACCTCCAAAGAGCTTAAGCAGTTCCAGAAACCTGAAAATGATTCATACCAGATAGATGCATTGGACCCTTCAAAATCAAGCGCTGGTATTGACTTCAAGGGTCAATTGTTTAAACTACTGCACCAGTTGGAGAGCAGCACCCCTCACTTCATTAGCTGCATAAAGCCAAACAGTAAGCAGCTTCCCGGCATGTATGAAGTAAACCTTGTTTTACAACAGCTCAAGTGTTGTGGAGTTTTGGAGGTTGTTAGAATATCACGATCTGGATACCCTACTAGAATGGCACATCAAGAATTTGCAGGAAGATATGGTTTTCTACTTTTGGAGGACGATTTACCTCGGGATCCATTAAGTCTATCCATTGCTGTTCTAAAAAGATTCAGTGTCCTCCCTGAGATGTACCAAATTGGCTATACGAAAGTGTTTCTTAGAACAGGGCAGATTGCATCATTGGAGGATAAGAGAAAGAAAGTTCTGAGAGGAATAATTGGTGTCCAAAAATACTTCCGTGGTCACCGGGCTCGTTGCCACTTTGATCAACTTAAGGAGGGAGTCCCAAAGATACCATCAAACGGTCTTGGTGAAAATACTGGAAGGAAAGCAGGTTCTTGTGAAACTCTTGATGAGCGTCAAAATTTGAAGAAGTCACATCCTGAGAATGGGAAAGCTAAAAGGAAGGCTGGCAGGAAGATGTCAGAGGTGAAGGATTTGCCATCAGATTTGGCAGAGCTTCAAAGGCGGGTGCTTCAGGCTGAGGCAACTCTGGTGCGGAAGGAGGAGGAAAATGCTGAATTACAGGAGCAACTGCAACAATTTGAAACAAGATGGTCAGAACATGAATCAAAGATGAAATCGATGCAGGACGTGTGGCAAAAGCAGATGGCGTCTTTACAGACGAGTCTTGCTGCAGCCAGAAATAGTCTTGCATCTGACAATACTGCTGGTCAACCTGGAAGACTAGGTTCTGTTTCATCACCCCGCTACGATTCTGAAGAAGCCACATCGATGGGATTCAATGGAGCAGGGCGTGAGGCTAATGGCAGTTCAAATGCAGTCAGCAGTCTGGTGAAGGAATTTGAGCAGCGGAGACACACATTCAATGATGATGCGAAAGCTCTAGTTGAGGTAAAACCTGGGCAGTCAGCTGCAAATATGAATCCTGAGGAAGATTTACGAAAACTTAAACACCGGTTTGAGTCCTGGAAGAAAGAGTACAAGGCAAGATTACGGGAAACAAAGACAAAGCTTCATAAAATTTGGCACTCAGAAGAGGAAAAACGACAGAGAAAATGGTGGGCAAAGATAGGCTCAAGAGCATTTAACTTTCTCAGTAAGCTACCTCCTTCTTCAGTAGCTACTCCTCTGTTTATATATCTCGCATCGCATATCGGTGAATCTTGGTGTGGTGTAGTATTATGGAGAGAAACTTGTAGATCTACCAAATTTTTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000146 GO:0000166 GO:0000278 GO:0000281 GO:0000302 GO:0000910 GO:0001891 GO:0001931 GO:0003674 GO:0003774 GO:0003779 GO:0003824 GO:0005488 GO:0005515 GO:0005524 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005768 GO:0005826 GO:0005829 GO:0005856 GO:0005886 GO:0005911 GO:0005938 GO:0006928 GO:0006935 GO:0006950 GO:0006970 GO:0006971 GO:0006979 GO:0006996 GO:0007010 GO:0007015 GO:0007033 GO:0007049 GO:0007154 GO:0008064 GO:0008092 GO:0008104 GO:0008144 GO:0008150 GO:0008360 GO:0009267 GO:0009506 GO:0009581 GO:0009582 GO:0009605 GO:0009612 GO:0009628 GO:0009636 GO:0009987 GO:0009991 GO:0010033 GO:0010035 GO:0010243 GO:0010639 GO:0012505 GO:0014070 GO:0014074 GO:0015629 GO:0016020 GO:0016043 GO:0016050 GO:0016459 GO:0016460 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017076 GO:0017111 GO:0022402 GO:0022603 GO:0022604 GO:0022607 GO:0030029 GO:0030030 GO:0030031 GO:0030036 GO:0030038 GO:0030048 GO:0030054 GO:0030139 GO:0030554 GO:0030587 GO:0030832 GO:0030833 GO:0030837 GO:0030863 GO:0030864 GO:0030865 GO:0030866 GO:0030898 GO:0031033 GO:0031034 GO:0031152 GO:0031154 GO:0031254 GO:0031268 GO:0031270 GO:0031333 GO:0031410 GO:0031667 GO:0031668 GO:0031669 GO:0031982 GO:0032009 GO:0032060 GO:0032153 GO:0032155 GO:0032271 GO:0032272 GO:0032502 GO:0032535 GO:0032553 GO:0032555 GO:0032559 GO:0032796 GO:0032956 GO:0032970 GO:0032982 GO:0032991 GO:0033036 GO:0033043 GO:0033275 GO:0033298 GO:0033554 GO:0034461 GO:0034622 GO:0035639 GO:0036094 GO:0040011 GO:0042221 GO:0042330 GO:0042493 GO:0042542 GO:0042594 GO:0042623 GO:0042641 GO:0042802 GO:0042803 GO:0042995 GO:0043167 GO:0043168 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043232 GO:0043254 GO:0043933 GO:0044085 GO:0044087 GO:0044422 GO:0044424 GO:0044425 GO:0044430 GO:0044444 GO:0044446 GO:0044448 GO:0044459 GO:0044464 GO:0044764 GO:0044877 GO:0045177 GO:0045179 GO:0045335 GO:0046677 GO:0046683 GO:0046847 GO:0046983 GO:0048519 GO:0048523 GO:0048856 GO:0048870 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0050982 GO:0051015 GO:0051017 GO:0051128 GO:0051129 GO:0051179 GO:0051301 GO:0051493 GO:0051494 GO:0051591 GO:0051606 GO:0051674 GO:0051703 GO:0051704 GO:0051716 GO:0055044 GO:0060327 GO:0060328 GO:0061572 GO:0061640 GO:0065003 GO:0065007 GO:0065008 GO:0070252 GO:0070938 GO:0071496 GO:0071840 GO:0071889 GO:0071944 GO:0090066 GO:0090702 GO:0097159 GO:0097204 GO:0097367 GO:0097435 GO:0097708 GO:0098630 GO:0098743 GO:0099120 GO:0099568 GO:0099738 GO:0110053 GO:0120025 GO:0120031 GO:0120036 GO:1901265 GO:1901363 GO:1901698 GO:1901700 GO:1902903 GO:1902904 GO:1903047 GO:1990753
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
IQ Myosin_N Myosin_head

Protein Analysis

937

Amino Acids

106.52

Weight (kDa)

8.73

Isoelectric Point (pI)

42.99

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1952
AccB1I GGYRCC 1 cut(s) 734
AccI GTMKAC 2 cut(s) 358, 895
AccII CGCG 1 cut(s) 496
AciI CCGC 5 cut(s) 579, 2098, 2128, 2338, 2443
AclWI GGATC 4 cut(s) 26, 879, 1697, 1710
AcoI YGGCCR 1 cut(s) 576
AcsI RAATTY 8 cut(s) 153, 803, 1172, 1652, 1993, 2432, 2613, 2801
AcuI CTGAAG 5 cut(s) 56, 329, 2090, 2370, 2691
AcyI GRCGYC 1 cut(s) 2243
AfaI GTAC 4 cut(s) 443, 877, 1760, 2573
AfeI AGCGCT 2 cut(s) 522, 1441
AfiI CCNNNNNNNGG 3 cut(s) 430, 1931, 2018
AflII CTTAAG 2 cut(s) 1373, 1901
AflIII ACRYGT 2 cut(s) 561, 2223
AgeI ACCGGT 1 cut(s) 2547
AjiI CACGTC 1 cut(s) 2224
AjnI CCWGG 5 cut(s) 87, 693, 2308, 2491, 2558
AloI GAACNNNNNNTCC 2 cut(s) 1948, 1980
Alw26I GTCTC 3 cut(s) 260, 763, 2440
AlwI GGATC 4 cut(s) 26, 879, 1697, 1710
AlwNI CAGNNNCTG 1 cut(s) 230
Ama87I CYCGRG 1 cut(s) 1698
Aor51HI AGCGCT 2 cut(s) 522, 1441
AoxI GGCC 2 cut(s) 113, 576
ApoI RAATTY 8 cut(s) 153, 803, 1172, 1652, 1993, 2432, 2613, 2801
ArsI GACNNNNNNTTYG 2 cut(s) 195, 227
AseI ATTAAT 2 cut(s) 846, 954
AsiGI ACCGGT 1 cut(s) 2547
Asp700I GAANNNNTTC 1 cut(s) 166
AspLEI GCGC 3 cut(s) 496, 523, 1442
AspS9I GGNCC 2 cut(s) 113, 1423
AsuC2I CCSGG 3 cut(s) 112, 1545, 1877
AsuHPI GGTGA 6 cut(s) 1865, 1952, 2071, 2325, 2437, 2762
AsuNHI GCTAGC 1 cut(s) 404
AvaI CYCGRG 1 cut(s) 1698
AvaII GGWCC 1 cut(s) 1423
AxyI CCTNAGG 2 cut(s) 1143, 2520
BamHI GGATCC 1 cut(s) 1702
BanI GGYRCC 1 cut(s) 734
BanII GRGCYC 1 cut(s) 1882
BarI GAAGNNNNNNTAC 2 cut(s) 2555, 2587
BbsI GAAGAC 1 cut(s) 2320
BbvCI CCTCAGC 1 cut(s) 2112
BccI CCATC 9 cut(s) 473, 673, 896, 1025, 1933, 2080, 2178, 2233, 2359
BceAI ACGGC 1 cut(s) 858
BciT130I CCWGG 5 cut(s) 89, 695, 2310, 2493, 2560
BciVI GTATCC 1 cut(s) 1619
BclI TGATCA 1 cut(s) 1894
BcnI CCSGG 3 cut(s) 112, 1545, 1877
BcoDI GTCTC 3 cut(s) 260, 763, 2440
BfaI CTAG 7 cut(s) 362, 405, 435, 524, 1635, 2318, 2477
BfmI CTRYAG 2 cut(s) 1349, 2265
BfoI RGCGCY 2 cut(s) 524, 1443
BfrI CTTAAG 2 cut(s) 1373, 1901
BfuAI ACCTGC 1 cut(s) 1952
BfuI GTATCC 1 cut(s) 1619
BglII AGATCT 1 cut(s) 2792
Bme1390I CCNGG 8 cut(s) 89, 112, 695, 1545, 1877, 2310, 2493, 2560
Bme18I GGWCC 1 cut(s) 1423
BmeT110I CYCGRG 1 cut(s) 1698
BmgBI CACGTC 1 cut(s) 2224
BmgT120I GGNCC 2 cut(s) 113, 1423
BmiI GGNNCC 3 cut(s) 736, 1425, 1704
BmrFI CCNGG 8 cut(s) 89, 112, 695, 1545, 1877, 2310, 2493, 2560
BmrI ACTGGG 1 cut(s) 888
BmtI GCTAGC 1 cut(s) 408
BmuI ACTGGG 1 cut(s) 888
BpiI GAAGAC 1 cut(s) 2320
BpmI CTGGAG 1 cut(s) 1269
Bpu10I CCTNAGC 1 cut(s) 2112
BpuEI CTTGAG 3 cut(s) 1028, 1565, 2653
BpuMI CCSGG 3 cut(s) 112, 1545, 1877
Bsa29I ATCGAT 4 cut(s) 21, 477, 2213, 2363
BsaBI GATNNNNATC 1 cut(s) 128
BsaHI GRCGYC 1 cut(s) 2243
BsaJI CCNNGG 8 cut(s) 87, 693, 730, 738, 795, 1697, 1867, 2492
BsaWI WCCGGW 1 cut(s) 2547
Bsc4I CCNNNNNNNGG 3 cut(s) 430, 1931, 2018
Bse118I RCCGGY 2 cut(s) 1341, 2547
Bse1I ACTGG 5 cut(s) 116, 833, 883, 1483, 1954
Bse21I CCTNAGG 2 cut(s) 1143, 2520
Bse3DI GCAATG 4 cut(s) 288, 569, 663, 1719
Bse8I GATNNNNATC 1 cut(s) 128
BseBI CCWGG 5 cut(s) 89, 695, 2310, 2493, 2560
BseCI ATCGAT 4 cut(s) 21, 477, 2213, 2363
BseDI CCNNGG 8 cut(s) 87, 693, 730, 738, 795, 1697, 1867, 2492
BseGI GGATG 4 cut(s) 386, 1036, 1129, 2008
BseJI GATNNNNATC 1 cut(s) 128
BseLI CCNNNNNNNGG 3 cut(s) 430, 1931, 2018
BseMI GCAATG 4 cut(s) 288, 569, 663, 1719
BseMII CTCAG 8 cut(s) 758, 1743, 1826, 2004, 2103, 2511, 2637, 2701
BseNI ACTGG 5 cut(s) 116, 833, 883, 1483, 1954
BseRI GAGGAG 2 cut(s) 2150, 2709
BseYI CCCAGC 2 cut(s) 254, 592
BsgI GTGCAG 2 cut(s) 1354, 1463
Bsh1236I CGCG 1 cut(s) 496
BshFI GGCC 2 cut(s) 115, 578
BshNI GGYRCC 1 cut(s) 734
BshTI ACCGGT 1 cut(s) 2547
BshVI ATCGAT 4 cut(s) 21, 477, 2213, 2363
BsiHKCI CYCGRG 1 cut(s) 1698
BsiSI CCGG 5 cut(s) 111, 1342, 1545, 1876, 2548
BslFI GGGAC 1 cut(s) 1898
BslI CCNNNNNNNGG 3 cut(s) 430, 1931, 2018
BsmAI GTCTC 3 cut(s) 260, 763, 2440
BsmFI GGGAC 1 cut(s) 1898
BsmI GAATGC 2 cut(s) 1079, 1195
BsnI GGCC 2 cut(s) 115, 578
BsoBI CYCGRG 1 cut(s) 1698
Bsp1286I GDGCHC 1 cut(s) 1882
Bsp143I GATC 8 cut(s) 18, 22, 550, 884, 1619, 1702, 1894, 2792
Bsp19I CCATGG 1 cut(s) 730
BspACI CCGC 5 cut(s) 579, 2098, 2128, 2338, 2443
BspANI GGCC 2 cut(s) 115, 578
BspCNI CTCAG 8 cut(s) 759, 1744, 1827, 2005, 2104, 2512, 2636, 2700
BspDI ATCGAT 4 cut(s) 21, 477, 2213, 2363
BspFNI CGCG 1 cut(s) 496
BspLI GGNNCC 3 cut(s) 736, 1425, 1704
BspMAI CTGCAG 2 cut(s) 1353, 2269
BspMI ACCTGC 1 cut(s) 1952
BspOI GCTAGC 1 cut(s) 408
BspPI GGATC 4 cut(s) 26, 879, 1697, 1710
BspT107I GGYRCC 1 cut(s) 734
BspTI CTTAAG 2 cut(s) 1373, 1901
BsrDI GCAATG 4 cut(s) 288, 569, 663, 1719
BsrFI RCCGGY 2 cut(s) 1341, 2547
BsrI ACTGG 5 cut(s) 116, 833, 883, 1483, 1954
BssAI RCCGGY 2 cut(s) 1341, 2547
BssECI CCNNGG 8 cut(s) 87, 693, 730, 738, 795, 1697, 1867, 2492
BssMI GATC 8 cut(s) 18, 22, 550, 884, 1619, 1702, 1894, 2792
BssNAI GTATAC 2 cut(s) 359, 896
BssNI GRCGYC 1 cut(s) 2243
BssT1I CCWWGG 3 cut(s) 730, 738, 795
Bst1107I GTATAC 2 cut(s) 359, 896
Bst2UI CCWGG 5 cut(s) 89, 695, 2310, 2493, 2560
Bst4CI ACNGT 2 cut(s) 1532, 1934
Bst6I CTCTTC 1 cut(s) 2622
BstACI GRCGYC 1 cut(s) 2243
BstAFI CTTAAG 2 cut(s) 1373, 1901
BstAPI GCANNNNNTGC 4 cut(s) 230, 278, 782, 791
BstC8I GCNNGC 6 cut(s) 54, 330, 406, 576, 1343, 2041
BstDSI CCRYGG 2 cut(s) 730, 1867
BstEII GGTNACC 1 cut(s) 1871
BstF5I GGATG 4 cut(s) 386, 1036, 1129, 2008
BstFNI CGCG 1 cut(s) 496
BstH2I RGCGCY 2 cut(s) 524, 1443
BstHHI GCGC 3 cut(s) 496, 523, 1442
BstKTI GATC 8 cut(s) 21, 25, 553, 887, 1622, 1705, 1897, 2795
BstMAI GTCTC 3 cut(s) 260, 763, 2440
BstMBI GATC 8 cut(s) 18, 22, 550, 884, 1619, 1702, 1894, 2792
BstMWI GCNNNNNNNGC 7 cut(s) 230, 278, 318, 653, 782, 791, 2095
BstNI CCWGG 5 cut(s) 89, 695, 2310, 2493, 2560
BstNSI RCATGY 4 cut(s) 332, 565, 635, 1552
BstPI GGTNACC 1 cut(s) 1871
BstSCI CCNGG 8 cut(s) 87, 110, 693, 1543, 1875, 2308, 2491, 2558
BstSFI CTRYAG 2 cut(s) 1349, 2265
BstUI CGCG 1 cut(s) 496
BstV2I GAAGAC 1 cut(s) 2320
BstX2I RGATCY 3 cut(s) 884, 1702, 2792
BstXI CCANNNNNNTGG 2 cut(s) 679, 2365
BstYI RGATCY 3 cut(s) 884, 1702, 2792
BstZ17I GTATAC 2 cut(s) 359, 896
Bsu15I ATCGAT 4 cut(s) 21, 477, 2213, 2363
Bsu36I CCTNAGG 2 cut(s) 1143, 2520
BsuI GTATCC 1 cut(s) 1619
BsuRI GGCC 2 cut(s) 115, 578
BsuTUI ATCGAT 4 cut(s) 21, 477, 2213, 2363
BtgI CCRYGG 2 cut(s) 730, 1867
BtgZI GCGATG 1 cut(s) 2724
BtrI CACGTC 1 cut(s) 2224
BtsCI GGATG 4 cut(s) 386, 1036, 1129, 2008
BtsI GCAGTG 2 cut(s) 588, 867
BtsIMutI CAGTG 5 cut(s) 80, 588, 764, 867, 1747
BveI ACCTGC 1 cut(s) 1952
Cac8I GCNNGC 6 cut(s) 54, 330, 406, 576, 1343, 2041
CaiI CAGNNNCTG 1 cut(s) 230
CfoI GCGC 3 cut(s) 496, 523, 1442
Cfr10I RCCGGY 2 cut(s) 1341, 2547
Cfr13I GGNCC 2 cut(s) 113, 1423
ClaI ATCGAT 4 cut(s) 21, 477, 2213, 2363
CseI GACGC 3 cut(s) 236, 1976, 2232
Csp6I GTAC 4 cut(s) 442, 876, 1759, 2572
CspAI ACCGGT 1 cut(s) 2547
CspCI CAANNNNNGTGG 2 cut(s) 1570, 1605
CviQI GTAC 4 cut(s) 442, 876, 1759, 2572
DpnI GATC 8 cut(s) 20, 24, 552, 886, 1621, 1704, 1896, 2794
DpnII GATC 8 cut(s) 18, 22, 550, 884, 1619, 1702, 1894, 2792
DraI TTTAAA 2 cut(s) 1207, 1471
EaeI YGGCCR 1 cut(s) 576
Eam1104I CTCTTC 1 cut(s) 2622
EarI CTCTTC 1 cut(s) 2622
Eco130I CCWWGG 3 cut(s) 730, 738, 795
Eco24I GRGCYC 1 cut(s) 1882
Eco47I GGWCC 1 cut(s) 1423
Eco47III AGCGCT 2 cut(s) 522, 1441
Eco57I CTGAAG 5 cut(s) 56, 329, 2090, 2370, 2691
Eco81I CCTNAGG 2 cut(s) 1143, 2520
Eco88I CYCGRG 1 cut(s) 1698
Eco91I GGTNACC 1 cut(s) 1871
EcoO65I GGTNACC 1 cut(s) 1871
EcoRII CCWGG 5 cut(s) 87, 693, 2308, 2491, 2558
EcoT14I CCWWGG 3 cut(s) 730, 738, 795
EcoT22I ATGCAT 1 cut(s) 1420
EcoT38I GRGCYC 1 cut(s) 1882
ErhI CCWWGG 3 cut(s) 730, 738, 795
FalI AAGNNNNNCTT 8 cut(s) 920, 952, 1169, 1201, 1548, 1580, 1770, 1802
FaqI GGGAC 1 cut(s) 1898
FauI CCCGC 2 cut(s) 2091, 2345
FbaI TGATCA 1 cut(s) 1894
FblI GTMKAC 2 cut(s) 358, 895
FokI GGATG 4 cut(s) 373, 1043, 1136, 1995
FriOI GRGCYC 1 cut(s) 1882
FspBI CTAG 7 cut(s) 362, 405, 435, 524, 1635, 2318, 2477
GlaI GCGC 3 cut(s) 495, 522, 1441
GsaI CCCAGC 2 cut(s) 258, 596
GsuI CTGGAG 1 cut(s) 1269
HaeII RGCGCY 2 cut(s) 524, 1443
HaeIII GGCC 2 cut(s) 115, 578
HapII CCGG 5 cut(s) 111, 1342, 1545, 1876, 2548
HgaI GACGC 3 cut(s) 236, 1976, 2232
HhaI GCGC 3 cut(s) 496, 523, 1442
Hin1I GRCGYC 1 cut(s) 2243
Hin6I GCGC 3 cut(s) 494, 521, 1440
HinP1I GCGC 3 cut(s) 494, 521, 1440
HincII GTYRAC 1 cut(s) 2306
HindII GTYRAC 1 cut(s) 2306
HindIII AAGCTT 2 cut(s) 1004, 2603
HpaII CCGG 5 cut(s) 111, 1342, 1545, 1876, 2548
HphI GGTGA 6 cut(s) 1865, 1952, 2071, 2325, 2437, 2762
Hpy166II GTNNAC 6 cut(s) 359, 717, 896, 1359, 1561, 2306
Hpy188I TCNGA 9 cut(s) 348, 459, 1836, 2056, 2077, 2191, 2290, 2350, 2626
Hpy8I GTNNAC 6 cut(s) 359, 717, 896, 1359, 1561, 2306
HpyCH4III ACNGT 2 cut(s) 1532, 1934
HpyCH4IV ACGT 1 cut(s) 2223
HpyF10VI GCNNNNNNNGC 7 cut(s) 230, 278, 318, 653, 782, 791, 2095
HpySE526I ACGT 1 cut(s) 2223
Hsp92I GRCGYC 1 cut(s) 2243
HspAI GCGC 3 cut(s) 494, 521, 1440
KroI GCCGGC 1 cut(s) 1341
KroNI GCCGGC 1 cut(s) 1343
Ksp22I TGATCA 1 cut(s) 1894
Kzo9I GATC 8 cut(s) 18, 22, 550, 884, 1619, 1702, 1894, 2792
LmnI GCTCC 3 cut(s) 697, 2158, 2378
MaeI CTAG 7 cut(s) 362, 405, 435, 524, 1635, 2318, 2477
MaeII ACGT 1 cut(s) 2223
MaeIII GTNAC 4 cut(s) 658, 852, 1871, 2004
MalI GATC 8 cut(s) 20, 24, 552, 886, 1621, 1704, 1896, 2794
MboI GATC 8 cut(s) 18, 22, 550, 884, 1619, 1702, 1894, 2792
MfeI CAATTG 3 cut(s) 352, 777, 1463
MflI RGATCY 3 cut(s) 884, 1702, 2792
MhlI GDGCHC 1 cut(s) 1882
MlyI GAGTC 4 cut(s) 926, 1920, 2266, 2564
MmeI TCCRAC 3 cut(s) 1101, 1342, 1467
Mph1103I ATGCAT 1 cut(s) 1420
MroNI GCCGGC 1 cut(s) 1341
MroXI GAANNNNTTC 1 cut(s) 166
MslI CAYNNNNRTG 6 cut(s) 213, 333, 677, 1188, 2363, 2748
MspA1I CMGCKG 3 cut(s) 254, 2443, 2504
MspCI CTTAAG 2 cut(s) 1373, 1901
MspI CCGG 5 cut(s) 111, 1342, 1545, 1876, 2548
MspR9I CCNGG 8 cut(s) 89, 112, 695, 1545, 1877, 2310, 2493, 2560
MssI GTTTAAAC 1 cut(s) 1471
MunI CAATTG 3 cut(s) 352, 777, 1463
Mva1269I GAATGC 2 cut(s) 1079, 1195
MvaI CCWGG 5 cut(s) 89, 695, 2310, 2493, 2560
MvnI CGCG 1 cut(s) 496
MwoI GCNNNNNNNGC 7 cut(s) 230, 278, 318, 653, 782, 791, 2095
NaeI GCCGGC 1 cut(s) 1343
NciI CCSGG 3 cut(s) 112, 1545, 1877
NcoI CCATGG 1 cut(s) 730
NdeII GATC 8 cut(s) 18, 22, 550, 884, 1619, 1702, 1894, 2792
NgoMIV GCCGGC 1 cut(s) 1341
NheI GCTAGC 1 cut(s) 404
NlaIV GGNNCC 3 cut(s) 736, 1425, 1704
NmuCI GTSAC 3 cut(s) 852, 1871, 2004
NsiI ATGCAT 1 cut(s) 1420
NspI RCATGY 4 cut(s) 332, 565, 635, 1552
PaeI GCATGC 1 cut(s) 332
PciI ACATGT 1 cut(s) 561
PctI GAATGC 2 cut(s) 1079, 1195
PdiI GCCGGC 1 cut(s) 1343
PdmI GAANNNNTTC 1 cut(s) 166
PfoI TCCNGGA 1 cut(s) 2558
PinAI ACCGGT 1 cut(s) 2547
PleI GAGTC 4 cut(s) 925, 1919, 2265, 2563
PmeI GTTTAAAC 1 cut(s) 1471
PpsI GAGTC 4 cut(s) 925, 1919, 2265, 2563
PscI ACATGT 1 cut(s) 561
PshBI ATTAAT 2 cut(s) 846, 954
Psp6I CCWGG 5 cut(s) 87, 693, 2308, 2491, 2558
PspEI GGTNACC 1 cut(s) 1871
PspFI CCCAGC 2 cut(s) 254, 592
PspGI CCWGG 5 cut(s) 87, 693, 2308, 2491, 2558
PspN4I GGNNCC 3 cut(s) 736, 1425, 1704
PspPI GGNCC 2 cut(s) 113, 1423
PstI CTGCAG 2 cut(s) 1353, 2269
PstNI CAGNNNCTG 1 cut(s) 230
PsuI RGATCY 3 cut(s) 884, 1702, 2792
PvuII CAGCTG 2 cut(s) 254, 2504
RsaI GTAC 4 cut(s) 443, 877, 1760, 2573
RsaNI GTAC 4 cut(s) 442, 876, 1759, 2572
RseI CAYNNNNRTG 6 cut(s) 213, 333, 677, 1188, 2363, 2748
Sau3AI GATC 8 cut(s) 18, 22, 550, 884, 1619, 1702, 1894, 2792
Sau96I GGNCC 2 cut(s) 113, 1423
SchI GAGTC 4 cut(s) 926, 1920, 2266, 2564
ScrFI CCNGG 8 cut(s) 89, 112, 695, 1545, 1877, 2310, 2493, 2560
SduI GDGCHC 1 cut(s) 1882
SfcI CTRYAG 2 cut(s) 1349, 2265
SinI GGWCC 1 cut(s) 1423
SmiMI CAYNNNNRTG 6 cut(s) 213, 333, 677, 1188, 2363, 2748
SmlI CTYRAG 5 cut(s) 1007, 1373, 1580, 1901, 2668
SmoI CTYRAG 5 cut(s) 1007, 1373, 1580, 1901, 2668
SphI GCATGC 1 cut(s) 332
SsiI CCGC 5 cut(s) 579, 2098, 2128, 2338, 2443
SspMI CTAG 7 cut(s) 362, 405, 435, 524, 1635, 2318, 2477
StyD4I CCNGG 8 cut(s) 87, 110, 693, 1543, 1875, 2308, 2491, 2558
StyI CCWWGG 3 cut(s) 730, 738, 795
TaaI ACNGT 2 cut(s) 1532, 1934
TaiI ACGT 1 cut(s) 2226
TaqI TCGA 7 cut(s) 8, 21, 477, 610, 1133, 2213, 2363
TatI WGTACW 2 cut(s) 441, 2571
TauI GCSGC 1 cut(s) 581
TscAI CASTG 5 cut(s) 80, 588, 771, 867, 1747
TseFI GTSAC 3 cut(s) 852, 1871, 2004
Tsp45I GTSAC 3 cut(s) 852, 1871, 2004
TspGWI ACGGA 1 cut(s) 1856
TspRI CASTG 5 cut(s) 80, 588, 771, 867, 1747
Vha464I CTTAAG 2 cut(s) 1373, 1901
VpaK11BI GGWCC 1 cut(s) 1423
VspI ATTAAT 2 cut(s) 846, 954
XapI RAATTY 8 cut(s) 153, 803, 1172, 1652, 1993, 2432, 2613, 2801
XceI RCATGY 4 cut(s) 332, 565, 635, 1552
XmiI GTMKAC 2 cut(s) 358, 895
XmnI GAANNNNTTC 1 cut(s) 166
XspI CTAG 7 cut(s) 362, 405, 435, 524, 1635, 2318, 2477
Zsp2I ATGCAT 1 cut(s) 1420
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.