MD11G1237000.v1.1
ERF Family

Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Myosin family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0005645
Physical Location & Seq
Reverse (-)
34457764 .. 34468648
10885 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1237000.v1.1.491

Sequence Viewer

Length: 3432 bp
ATGATGGCTTCCGCGTCTCCGTCGATGATAGCGAGGAGCTCGTTGGAGGAAATGCTGGAGTCGCTTCAGCGAAGAGATACGGTCGAAAAGCCGAAGGAATTGCCGCCGGCATTACCAGCTCGGCCGCCTTCCAAGGCTCGTCTGCCATCAGCCCGCCGCTCTATGCCCAACAACTTCAGGGTCGAAGACGCAGAGGGTTCTCTGGAGTGTTTACCTAGTTTGAATAAGAGAAAAGAGGGGGACTTGGGGTTCAAGGCAGGTCATTTTGGCGTGAAGAAGACGGAGAATGATCAAACTGTTGAGTCGCCCTACGGTGGGTCACCTGAGGACAGTGGAACGAGGCCGGAGAAGATTGCAAAATCGGATTGCGATGACAACATTGGTTATTTCATCAAGAAGAAACTTCGTGTTTGGTGTTGGCTACCTAGTGGGCTGTGGGAGTTAGGAATGATACAGTCAACTTCAGGGGACGCTGCACTTGTTTCACTCTCTAGTGGAAATGTAATCAAAGTGCACAGAGTTGACCTCTTACCTGCAAACCCAGATGTTCTCGAAGGTGTGGATGATCTTATACAGCTTAGTTATTTGAATGAGCCTGCTGTTCTTTACAATCTTCAGTGTAGATATTCCCAGGATATGATTTATAGCAAAGCCGGGCCAGTTTTGATTGCAATCAATCCTTTCAAAGATGTTCAAATTTATGGAAAAGATTCTATTAGAGCTTATAGGCAGAAACTAACAGACAAACCTCATGTCTATGCTGTGGCAGATGCTGCTTATGACGAGATGATGACAGATGATGTAAATCAGTCCATAATCATAAGTGGGGAAAGTGGAGCTGGGAAAACTGAGACAGCAAAAGTTGCAATGCAATACTTGGCTGCTATTGGTAGTGGCAGTTGTGGCACAGAGCATGCAAATGCAATCCTTCAGACCAATTGTATACTAGAAGCGTTTGGAAATGCAAAAACATCCAGAAATCATAATGCTAGCCGATTCGGGAAATTGATTGAACTTCATTTTAGCACTGCGGGAAGAATATGTGGTGCTTTTATCCAAACATTTTTTTTGGACAAGTCCAGAGTTGCTCAACTAGAAAACGGTGAGAGGTCGTATCATGTCTTTTATCAACTCTGTGCTGGCGCTCCATCAACGCTAAAAGAGAGGCTAAACCTTAAAAGGGCTAGTGAGTACAAGTATCTTAATCAGAGTGATTGTTTGGAAATTGATGGTGTAGATGATGTGCGAAAGTTTCATACACTTGTGAAAGCGCTAGATGTTGTTCAAGTTTGTAAGGAAGATCAAGAACACATGTTTTCATTGCTGGCCGCAGTGTTATGGCTGGGAAACATATCATTTCAAGCGATTGACAATGAGAAGCATGTGGAAGTGTTAGCTGATGAAGCTGTAACCATTGCTTCCATGCTGATGGGTTGTAGTTCCCAGGAGCTAATTCTTTCTTTGTCCACCCCCGAAATCCGTGGTGCCAAGGATAGTATTGACACAAGGCTGACACTGAGACAGGCAATTGATGCAAGAGATGCGTTGGCAAAATTTATCTATGTTAGCTTGTTTGACTGGCTTGTAGAACAAATTAATAAGTCACTTGCAGTGGGAAAATGCCGTGCTGGGAGATCCATCAGTATACTTGATATTTATGGGTTTGAGTCATTCCAGAAGAACAGCTTTGAACAAATGTGCATTAATTATGCAAATGAGAGGCTGCAACAACATTTCAACCGACATTTATTTAAGCTTGAGCAGGAGGAATGTGAATTGGATGGGGTTGATTGGACTAAAGTTGATTTTCAAGACAATCAAGAATGCTTGAATCTGTTTGAGAAGAAACCTTTTGGGCTACTATCTGTGTTGGATGAGGAATCGAACTTCCCTAAGGCAAATGATTTGACCCTTGCCAATAAATTTAAGCAACACTTGAATGCTAATTCTTGCTTTAAAGCAGAAAAAGGAAGCACTTTCAGTATTCGTCATCATGCTGGAGAGGTTCTATATGACACAAGTGGCTTCCTAAAAAAAAACAGAGATAAGTTGCCTGCTGTTTTCGTCCAACTCCTGTCATCGTGCAGATGCCGGCTTCTGCAGTTGTTTACTTCCAAAGAGCTTAAGCAGTTCCAGAAACCTGAAAATGATTTGTACCAGATAGATGCATTGGACCCTTCAAAATCAGGCGCTGGTATTGACTTCAAGGGTCAATTGTTTAAACTACTGCACCAGTTGGAGAGCAGCACCCCTCACTTCATTAGCTGCATAAAGCCAAACAGTAAGCAGCTTCCCGGCATGTATGAAGTAAACCTTGTTTTACAACAGCTCAAGTGTTGTGGAGTTTTGGAGGTTGTTAGGATATCACGATCTGGATACCCTACTAGAATGGCGCATCAAGAATTTGCAGGAAGGTATGGTTTTCTACTTTTGGAGGACGATTTACCTCGGGATCCATTAAGTCTATCCATTGCTGTTCTAAAAAGATTCAGTGTCCTCCCTGAGATGTACCAAATTGGCTATACAAAAGTGTTTCTTAGAACAGGGCAGATTGCATCATTGGAGGATAAGAGAAAGAAAGTTCTGAGAGGAATAATTGGTGTCCAAAAATACTTCCGTGGTCACCGGGCTCGTTGCCACTTTGATCAACTTAAGGAAGGAGTCCCAAAGATACCATCAAACGGTCTTGGTGAAAATACTGGAAGGAAAGCAGGTTCTCATGAAACTCTTGATGAGCGTCAAAATTTGAAGAAGTCACATCCTGAGAATGGGAAAGCTAAAAGGAAGGCCGGCAGGAAGATGTCAGAGGTGAAGGATTTGCCATCAGATTTGGCAGAGCTTCAAAGGCGGGTGCTTCAGGCTGAGGCAACTCTGGTGCGGAAGGAGGAGGAAAATGCTGAATTACAGGAGCAACTGCAGCAATTTGAAACAAGATGGTCAGAATATGAATCAAAGATGAAATCGATGCAGGATGTGTGGCAAAAGCAGATGGCGTCTTTACAGACGAGTCTTGCTGCAGCCAGAAAGAGTCTTGCATCTGACAATACTGCTGGTCAACCTGGAAGACTAGGTTCTGTTTCATCACCCCGATACGATTCTGAAGAAGCCACATCCATGGGATTCAATGGAGCAGGGCTTGAGGCTAATGGCAGTTCAAATGCAGTCAGCGGTCTGGTGAAGGAATTTGAGCAGCGGAGACAGACATTCGATGATGATGCGAAAGCTCTAGTTGAGGTAAAACCTGGGCAGTCAGCTGCAAATATGAATCCTGAGGAAGATTTACGAAAACTTAAACACCGGTTTGAGTCCTGGAAGAAAGAGTACAAGGCAAGATTACGGGAAACAAAGACAAAGCTTCATAAAATTTGGCACTCAGAAGAGGAAAAACGACAGAGAAAATGGTGGGCAAAGATAGGCTCAAGAGCATTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000146 GO:0000166 GO:0000278 GO:0000281 GO:0000302 GO:0000910 GO:0001891 GO:0001931 GO:0003674 GO:0003774 GO:0003779 GO:0003824 GO:0005488 GO:0005515 GO:0005524 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005768 GO:0005826 GO:0005829 GO:0005856 GO:0005886 GO:0005911 GO:0005938 GO:0006928 GO:0006935 GO:0006950 GO:0006970 GO:0006971 GO:0006979 GO:0006996 GO:0007010 GO:0007015 GO:0007033 GO:0007049 GO:0007154 GO:0008064 GO:0008092 GO:0008104 GO:0008144 GO:0008150 GO:0008360 GO:0009267 GO:0009506 GO:0009581 GO:0009582 GO:0009605 GO:0009612 GO:0009628 GO:0009636 GO:0009987 GO:0009991 GO:0010033 GO:0010035 GO:0010243 GO:0010639 GO:0012505 GO:0014070 GO:0014074 GO:0015629 GO:0016020 GO:0016043 GO:0016050 GO:0016459 GO:0016460 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017076 GO:0017111 GO:0022402 GO:0022603 GO:0022604 GO:0022607 GO:0030029 GO:0030030 GO:0030031 GO:0030036 GO:0030038 GO:0030048 GO:0030054 GO:0030139 GO:0030554 GO:0030587 GO:0030832 GO:0030833 GO:0030837 GO:0030863 GO:0030864 GO:0030865 GO:0030866 GO:0030898 GO:0031033 GO:0031034 GO:0031152 GO:0031154 GO:0031254 GO:0031268 GO:0031270 GO:0031333 GO:0031410 GO:0031667 GO:0031668 GO:0031669 GO:0031982 GO:0032009 GO:0032060 GO:0032153 GO:0032155 GO:0032271 GO:0032272 GO:0032502 GO:0032535 GO:0032553 GO:0032555 GO:0032559 GO:0032796 GO:0032956 GO:0032970 GO:0032982 GO:0032991 GO:0033036 GO:0033043 GO:0033275 GO:0033298 GO:0033554 GO:0034461 GO:0034622 GO:0035639 GO:0036094 GO:0040011 GO:0042221 GO:0042330 GO:0042493 GO:0042542 GO:0042594 GO:0042623 GO:0042641 GO:0042802 GO:0042803 GO:0042995 GO:0043167 GO:0043168 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043232 GO:0043254 GO:0043933 GO:0044085 GO:0044087 GO:0044422 GO:0044424 GO:0044425 GO:0044430 GO:0044444 GO:0044446 GO:0044448 GO:0044459 GO:0044464 GO:0044764 GO:0044877 GO:0045177 GO:0045179 GO:0045335 GO:0046677 GO:0046683 GO:0046847 GO:0046983 GO:0048519 GO:0048523 GO:0048856 GO:0048870 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0050982 GO:0051015 GO:0051017 GO:0051128 GO:0051129 GO:0051179 GO:0051301 GO:0051493 GO:0051494 GO:0051591 GO:0051606 GO:0051674 GO:0051703 GO:0051704 GO:0051716 GO:0055044 GO:0060327 GO:0060328 GO:0061572 GO:0061640 GO:0065003 GO:0065007 GO:0065008 GO:0070252 GO:0070938 GO:0071496 GO:0071840 GO:0071889 GO:0071944 GO:0090066 GO:0090702 GO:0097159 GO:0097204 GO:0097367 GO:0097435 GO:0097708 GO:0098630 GO:0098743 GO:0099120 GO:0099568 GO:0099738 GO:0110053 GO:0120025 GO:0120031 GO:0120036 GO:1901265 GO:1901363 GO:1901698 GO:1901700 GO:1902903 GO:1902904 GO:1903047 GO:1990753
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
IQ Myosin_N Myosin_head

Protein Analysis

1144

Amino Acids

128.62

Weight (kDa)

8.45

Isoelectric Point (pI)

45.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SH3_VIII-1_N PF25369 135 - 179 4.2e-19 Myosin-1-like, N-terminal SH3 domain
Myosin_head PF00063 187 - 847 6.5e-224 Myosin head (motor domain)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 3 cut(s) 248, 541, 2702
AccB1I GGYRCC 1 cut(s) 1484
AccBSI CCGCTC 1 cut(s) 159
AccI GTMKAC 2 cut(s) 943, 1645
AccII CGCG 1 cut(s) 14
AclWI GGATC 3 cut(s) 1629, 2447, 2460
AcoI YGGCCR 2 cut(s) 122, 1326
AcsI RAATTY 7 cut(s) 696, 1553, 1922, 2402, 2743, 3182, 3363
AcuI CTGAAG 7 cut(s) 50, 160, 447, 599, 914, 2840, 3120
AcyI GRCGYC 1 cut(s) 2993
AfaI GTAC 4 cut(s) 1193, 2156, 2510, 3323
AfeI AGCGCT 1 cut(s) 1272
AfiI CCNNNNNNNGG 5 cut(s) 314, 315, 1180, 2681, 2768
AflII CTTAAG 2 cut(s) 2123, 2651
AflIII ACRYGT 1 cut(s) 1311
AgeI ACCGGT 1 cut(s) 3297
AjnI CCWGG 5 cut(s) 630, 1443, 3058, 3241, 3308
AloI GAACNNNNNNTCC 4 cut(s) 233, 265, 2698, 2730
Alw21I GWGCWC 2 cut(s) 41, 516
Alw26I GTCTC 4 cut(s) 21, 845, 1513, 3190
Alw44I GTGCAC 1 cut(s) 512
AlwI GGATC 3 cut(s) 1629, 2447, 2460
AlwNI CAGNNNCTG 2 cut(s) 773, 2192
Ama87I CYCGRG 1 cut(s) 2448
Aor51HI AGCGCT 1 cut(s) 1272
AoxI GGCC 5 cut(s) 122, 341, 656, 1326, 2787
ApaLI GTGCAC 1 cut(s) 512
ApoI RAATTY 7 cut(s) 696, 1553, 1922, 2402, 2743, 3182, 3363
ArsI GACNNNNNNTTYG 2 cut(s) 738, 770
AseI ATTAAT 2 cut(s) 1596, 1704
AsiGI ACCGGT 1 cut(s) 3297
Asp700I GAANNNNTTC 1 cut(s) 709
AspLEI GCGC 4 cut(s) 1145, 1273, 2192, 2395
AspS9I GGNCC 2 cut(s) 656, 2173
AsuC2I CCSGG 3 cut(s) 655, 2295, 2627
AsuHPI GGTGA 7 cut(s) 312, 1115, 2615, 2702, 2821, 3075, 3187
AsuNHI GCTAGC 1 cut(s) 989
AvaI CYCGRG 1 cut(s) 2448
AvaII GGWCC 1 cut(s) 2173
AxyI CCTNAGG 3 cut(s) 324, 1893, 3270
BaeGI GKGCMC 1 cut(s) 516
BamHI GGATCC 1 cut(s) 2452
BanI GGYRCC 1 cut(s) 1484
BanII GRGCYC 2 cut(s) 41, 2632
BarI GAAGNNNNNNTAC 2 cut(s) 3305, 3337
BbsI GAAGAC 3 cut(s) 192, 284, 3070
Bbv12I GWGCWC 2 cut(s) 41, 516
BbvCI CCTCAGC 1 cut(s) 2862
BceAI ACGGC 1 cut(s) 1608
BcgI CGANNNNNNTGC 4 cut(s) 82, 116, 3197, 3231
BciT130I CCWGG 5 cut(s) 632, 1445, 3060, 3243, 3310
BciVI GTATCC 1 cut(s) 2369
BclI TGATCA 2 cut(s) 289, 2644
BcnI CCSGG 3 cut(s) 655, 2295, 2627
BcoDI GTCTC 4 cut(s) 21, 845, 1513, 3190
BfmI CTRYAG 3 cut(s) 2099, 2915, 3015
BfoI RGCGCY 3 cut(s) 1146, 1274, 2193
BfrI CTTAAG 2 cut(s) 2123, 2651
BfuAI ACCTGC 3 cut(s) 248, 541, 2702
BfuI GTATCC 1 cut(s) 2369
Bme1390I CCNGG 8 cut(s) 632, 655, 1445, 2295, 2627, 3060, 3243, 3310
Bme18I GGWCC 1 cut(s) 2173
BmeT110I CYCGRG 1 cut(s) 2448
BmgT120I GGNCC 2 cut(s) 656, 2173
BmiI GGNNCC 3 cut(s) 1486, 2175, 2454
BmrFI CCNGG 8 cut(s) 632, 655, 1445, 2295, 2627, 3060, 3243, 3310
BmtI GCTAGC 1 cut(s) 993
BpiI GAAGAC 3 cut(s) 192, 284, 3070
BplI GAGNNNNNCTC 2 cut(s) 510, 542
BpmI CTGGAG 3 cut(s) 77, 224, 2019
Bpu10I CCTNAGC 1 cut(s) 2862
BpuEI CTTGAG 4 cut(s) 1778, 2315, 3158, 3403
BpuMI CCSGG 3 cut(s) 655, 2295, 2627
Bsa29I ATCGAT 1 cut(s) 2963
BsaBI GATNNNNATC 2 cut(s) 671, 804
BsaHI GRCGYC 1 cut(s) 2993
BsaJI CCNNGG 9 cut(s) 132, 630, 1443, 1480, 1488, 2447, 2617, 3114, 3242
BsaWI WCCGGW 1 cut(s) 3297
Bsc4I CCNNNNNNNGG 5 cut(s) 314, 315, 1180, 2681, 2768
Bse118I RCCGGY 4 cut(s) 106, 2091, 2789, 3297
Bse1I ACTGG 4 cut(s) 659, 1583, 2233, 2704
Bse21I CCTNAGG 3 cut(s) 324, 1893, 3270
Bse3DI GCAATG 4 cut(s) 873, 1319, 1413, 2469
Bse8I GATNNNNATC 2 cut(s) 671, 804
BseBI CCWGG 5 cut(s) 632, 1445, 3060, 3243, 3310
BseCI ATCGAT 1 cut(s) 2963
BseDI CCNNGG 9 cut(s) 132, 630, 1443, 1480, 1488, 2447, 2617, 3114, 3242
BseGI GGATG 7 cut(s) 568, 971, 1786, 1879, 2758, 2977, 3110
BseJI GATNNNNATC 2 cut(s) 671, 804
BseLI CCNNNNNNNGG 5 cut(s) 314, 315, 1180, 2681, 2768
BseMI GCAATG 4 cut(s) 873, 1319, 1413, 2469
BseMII CTCAG 9 cut(s) 315, 840, 1508, 2493, 2576, 2754, 2853, 3261, 3387
BseNI ACTGG 4 cut(s) 659, 1583, 2233, 2704
BseRI GAGGAG 2 cut(s) 49, 2900
BseSI GKGCMC 1 cut(s) 516
BseX3I CGGCCG 1 cut(s) 122
BseYI CCCAGC 3 cut(s) 839, 1342, 1628
BsgI GTGCAG 3 cut(s) 459, 2104, 2213
Bsh1236I CGCG 1 cut(s) 14
Bsh1285I CGRYCG 2 cut(s) 84, 125
BshFI GGCC 5 cut(s) 124, 343, 658, 1328, 2789
BshNI GGYRCC 1 cut(s) 1484
BshTI ACCGGT 1 cut(s) 3297
BshVI ATCGAT 1 cut(s) 2963
BsiEI CGRYCG 2 cut(s) 84, 125
BsiHKAI GWGCWC 2 cut(s) 41, 516
BsiHKCI CYCGRG 1 cut(s) 2448
BsiSI CCGG 8 cut(s) 107, 344, 654, 2092, 2295, 2626, 2790, 3298
BslFI GGGAC 3 cut(s) 254, 482, 2648
BslI CCNNNNNNNGG 5 cut(s) 314, 315, 1180, 2681, 2768
BsmAI GTCTC 4 cut(s) 21, 845, 1513, 3190
BsmBI CGTCTC 1 cut(s) 21
BsmFI GGGAC 3 cut(s) 254, 482, 2648
BsmI GAATGC 2 cut(s) 1829, 1945
BsnI GGCC 5 cut(s) 124, 343, 658, 1328, 2789
BsoBI CYCGRG 1 cut(s) 2448
Bsp1286I GDGCHC 3 cut(s) 41, 516, 2632
Bsp143I GATC 7 cut(s) 289, 565, 1300, 1634, 2369, 2452, 2644
Bsp19I CCATGG 1 cut(s) 3114
BspANI GGCC 5 cut(s) 124, 343, 658, 1328, 2789
BspCNI CTCAG 9 cut(s) 316, 841, 1509, 2494, 2577, 2755, 2854, 3262, 3386
BspDI ATCGAT 1 cut(s) 2963
BspFNI CGCG 1 cut(s) 14
BspHI TCATGA 1 cut(s) 2719
BspLI GGNNCC 3 cut(s) 1486, 2175, 2454
BspMAI CTGCAG 3 cut(s) 2103, 2919, 3019
BspMI ACCTGC 3 cut(s) 248, 541, 2702
BspOI GCTAGC 1 cut(s) 993
BspPI GGATC 3 cut(s) 1629, 2447, 2460
BspT107I GGYRCC 1 cut(s) 1484
BspTI CTTAAG 2 cut(s) 2123, 2651
BsrBI CCGCTC 1 cut(s) 159
BsrDI GCAATG 4 cut(s) 873, 1319, 1413, 2469
BsrFI RCCGGY 4 cut(s) 106, 2091, 2789, 3297
BsrI ACTGG 4 cut(s) 659, 1583, 2233, 2704
BssAI RCCGGY 4 cut(s) 106, 2091, 2789, 3297
BssECI CCNNGG 9 cut(s) 132, 630, 1443, 1480, 1488, 2447, 2617, 3114, 3242
BssMI GATC 7 cut(s) 289, 565, 1300, 1634, 2369, 2452, 2644
BssNAI GTATAC 2 cut(s) 944, 1646
BssNI GRCGYC 1 cut(s) 2993
BssT1I CCWWGG 3 cut(s) 132, 1488, 3114
Bst1107I GTATAC 2 cut(s) 944, 1646
Bst2UI CCWGG 5 cut(s) 632, 1445, 3060, 3243, 3310
Bst4CI ACNGT 8 cut(s) 82, 298, 314, 332, 456, 1103, 2282, 2684
Bst6I CTCTTC 2 cut(s) 67, 3372
BstACI GRCGYC 1 cut(s) 2993
BstAFI CTTAAG 2 cut(s) 2123, 2651
BstAPI GCANNNNNTGC 4 cut(s) 773, 863, 1532, 1541
BstDSI CCRYGG 3 cut(s) 1480, 2617, 3114
BstEII GGTNACC 2 cut(s) 318, 2621
BstF5I GGATG 7 cut(s) 568, 971, 1786, 1879, 2758, 2977, 3110
BstFNI CGCG 1 cut(s) 14
BstH2I RGCGCY 3 cut(s) 1146, 1274, 2193
BstHHI GCGC 4 cut(s) 1145, 1273, 2192, 2395
BstKTI GATC 7 cut(s) 292, 568, 1303, 1637, 2372, 2455, 2647
BstMAI GTCTC 4 cut(s) 21, 845, 1513, 3190
BstMBI GATC 7 cut(s) 289, 565, 1300, 1634, 2369, 2452, 2644
BstMCI CGRYCG 2 cut(s) 84, 125
BstNI CCWGG 5 cut(s) 632, 1445, 3060, 3243, 3310
BstNSI RCATGY 4 cut(s) 917, 1315, 1385, 2302
BstPI GGTNACC 2 cut(s) 318, 2621
BstSCI CCNGG 8 cut(s) 630, 653, 1443, 2293, 2625, 3058, 3241, 3308
BstSFI CTRYAG 3 cut(s) 2099, 2915, 3015
BstSLI GKGCMC 1 cut(s) 516
BstUI CGCG 1 cut(s) 14
BstV2I GAAGAC 3 cut(s) 192, 284, 3070
BstX2I RGATCY 2 cut(s) 1634, 2452
BstXI CCANNNNNNTGG 2 cut(s) 1429, 3115
BstYI RGATCY 2 cut(s) 1634, 2452
BstZ17I GTATAC 2 cut(s) 944, 1646
BstZI CGGCCG 1 cut(s) 122
Bsu15I ATCGAT 1 cut(s) 2963
Bsu36I CCTNAGG 3 cut(s) 324, 1893, 3270
BsuI GTATCC 1 cut(s) 2369
BsuRI GGCC 5 cut(s) 124, 343, 658, 1328, 2789
BsuTUI ATCGAT 1 cut(s) 2963
BtgI CCRYGG 3 cut(s) 1480, 2617, 3114
BtgZI GCGATG 1 cut(s) 384
BtsCI GGATG 7 cut(s) 568, 971, 1786, 1879, 2758, 2977, 3110
BtsI GCAGTG 3 cut(s) 1026, 1338, 1617
BtsIMutI CAGTG 7 cut(s) 337, 623, 1026, 1338, 1514, 1617, 2497
BveI ACCTGC 3 cut(s) 248, 541, 2702
CaiI CAGNNNCTG 2 cut(s) 773, 2192
CciI TCATGA 1 cut(s) 2719
CfoI GCGC 4 cut(s) 1145, 1273, 2192, 2395
Cfr10I RCCGGY 4 cut(s) 106, 2091, 2789, 3297
Cfr13I GGNCC 2 cut(s) 656, 2173
ClaI ATCGAT 1 cut(s) 2963
CseI GACGC 5 cut(s) 3, 197, 479, 2726, 2982
Csp6I GTAC 4 cut(s) 1192, 2155, 2509, 3322
CspAI ACCGGT 1 cut(s) 3297
CspCI CAANNNNNGTGG 2 cut(s) 2320, 2355
CviQI GTAC 4 cut(s) 1192, 2155, 2509, 3322
DpnI GATC 7 cut(s) 291, 567, 1302, 1636, 2371, 2454, 2646
DpnII GATC 7 cut(s) 289, 565, 1300, 1634, 2369, 2452, 2644
DraI TTTAAA 2 cut(s) 1957, 2221
EaeI YGGCCR 2 cut(s) 122, 1326
EagI CGGCCG 1 cut(s) 122
Eam1104I CTCTTC 2 cut(s) 67, 3372
EarI CTCTTC 2 cut(s) 67, 3372
Ecl136II GAGCTC 1 cut(s) 39
EclXI CGGCCG 1 cut(s) 122
Eco130I CCWWGG 3 cut(s) 132, 1488, 3114
Eco24I GRGCYC 2 cut(s) 41, 2632
Eco32I GATATC 1 cut(s) 2364
Eco47I GGWCC 1 cut(s) 2173
Eco47III AGCGCT 1 cut(s) 1272
Eco52I CGGCCG 1 cut(s) 122
Eco53kI GAGCTC 1 cut(s) 39
Eco57I CTGAAG 7 cut(s) 50, 160, 447, 599, 914, 2840, 3120
Eco81I CCTNAGG 3 cut(s) 324, 1893, 3270
Eco88I CYCGRG 1 cut(s) 2448
Eco91I GGTNACC 2 cut(s) 318, 2621
EcoICRI GAGCTC 1 cut(s) 39
EcoO65I GGTNACC 2 cut(s) 318, 2621
EcoRII CCWGG 5 cut(s) 630, 1443, 3058, 3241, 3308
EcoRV GATATC 1 cut(s) 2364
EcoT14I CCWWGG 3 cut(s) 132, 1488, 3114
EcoT22I ATGCAT 1 cut(s) 2170
EcoT38I GRGCYC 2 cut(s) 41, 2632
ErhI CCWWGG 3 cut(s) 132, 1488, 3114
Esp3I CGTCTC 1 cut(s) 21
FalI AAGNNNNNCTT 8 cut(s) 1670, 1702, 1919, 1951, 2298, 2330, 2520, 2552
FaqI GGGAC 3 cut(s) 254, 482, 2648
FauI CCCGC 3 cut(s) 161, 1024, 2841
FbaI TGATCA 2 cut(s) 289, 2644
FblI GTMKAC 2 cut(s) 943, 1645
FokI GGATG 7 cut(s) 575, 958, 1793, 1886, 2745, 2984, 3097
FriOI GRGCYC 2 cut(s) 41, 2632
GlaI GCGC 4 cut(s) 1144, 1272, 2191, 2394
GsaI CCCAGC 3 cut(s) 843, 1346, 1632
GsuI CTGGAG 3 cut(s) 77, 224, 2019
HaeII RGCGCY 3 cut(s) 1146, 1274, 2193
HaeIII GGCC 5 cut(s) 124, 343, 658, 1328, 2789
HapII CCGG 8 cut(s) 107, 344, 654, 2092, 2295, 2626, 2790, 3298
HgaI GACGC 5 cut(s) 3, 197, 479, 2726, 2982
HhaI GCGC 4 cut(s) 1145, 1273, 2192, 2395
Hin1I GRCGYC 1 cut(s) 2993
Hin6I GCGC 4 cut(s) 1143, 1271, 2190, 2393
HinP1I GCGC 4 cut(s) 1143, 1271, 2190, 2393
HincII GTYRAC 3 cut(s) 459, 523, 3056
HindII GTYRAC 3 cut(s) 459, 523, 3056
HindIII AAGCTT 2 cut(s) 1754, 3353
HpaII CCGG 8 cut(s) 107, 344, 654, 2092, 2295, 2626, 2790, 3298
HphI GGTGA 7 cut(s) 312, 1115, 2615, 2702, 2821, 3075, 3187
Hpy99I CGWCG 1 cut(s) 25
HpyCH4III ACNGT 8 cut(s) 82, 298, 314, 332, 456, 1103, 2282, 2684
Hsp92I GRCGYC 1 cut(s) 2993
HspAI GCGC 4 cut(s) 1143, 1271, 2190, 2393
KroI GCCGGC 3 cut(s) 106, 2091, 2789
KroNI GCCGGC 3 cut(s) 108, 2093, 2791
Ksp22I TGATCA 2 cut(s) 289, 2644
Kzo9I GATC 7 cut(s) 289, 565, 1300, 1634, 2369, 2452, 2644
LmnI GCTCC 6 cut(s) 36, 836, 1150, 1447, 2908, 3128
MaeIII GTNAC 5 cut(s) 318, 1408, 1602, 2621, 2754
MalI GATC 7 cut(s) 291, 567, 1302, 1636, 2371, 2454, 2646
MbiI CCGCTC 1 cut(s) 159
MboI GATC 7 cut(s) 289, 565, 1300, 1634, 2369, 2452, 2644
MfeI CAATTG 3 cut(s) 937, 1527, 2213
MflI RGATCY 2 cut(s) 1634, 2452
MhlI GDGCHC 3 cut(s) 41, 516, 2632
MlyI GAGTC 7 cut(s) 68, 311, 1676, 2670, 3016, 3037, 3314
MmeI TCCRAC 4 cut(s) 24, 1851, 2092, 2217
Mph1103I ATGCAT 1 cut(s) 2170
MroNI GCCGGC 3 cut(s) 106, 2091, 2789
MroXI GAANNNNTTC 1 cut(s) 709
MslI CAYNNNNRTG 5 cut(s) 756, 918, 1427, 1938, 3113
MspA1I CMGCKG 3 cut(s) 3168, 3193, 3254
MspCI CTTAAG 2 cut(s) 2123, 2651
MspI CCGG 8 cut(s) 107, 344, 654, 2092, 2295, 2626, 2790, 3298
MspR9I CCNGG 8 cut(s) 632, 655, 1445, 2295, 2627, 3060, 3243, 3310
MssI GTTTAAAC 1 cut(s) 2221
MunI CAATTG 3 cut(s) 937, 1527, 2213
Mva1269I GAATGC 2 cut(s) 1829, 1945
MvaI CCWGG 5 cut(s) 632, 1445, 3060, 3243, 3310
MvnI CGCG 1 cut(s) 14
NaeI GCCGGC 3 cut(s) 108, 2093, 2791
NciI CCSGG 3 cut(s) 655, 2295, 2627
NcoI CCATGG 1 cut(s) 3114
NdeII GATC 7 cut(s) 289, 565, 1300, 1634, 2369, 2452, 2644
NgoMIV GCCGGC 3 cut(s) 106, 2091, 2789
NheI GCTAGC 1 cut(s) 989
NlaIV GGNNCC 3 cut(s) 1486, 2175, 2454
NmeAIII GCCGAG 1 cut(s) 100
NmuCI GTSAC 4 cut(s) 318, 1602, 2621, 2754
NsiI ATGCAT 1 cut(s) 2170
NspI RCATGY 4 cut(s) 917, 1315, 1385, 2302
PaeI GCATGC 1 cut(s) 917
PagI TCATGA 1 cut(s) 2719
PciI ACATGT 1 cut(s) 1311
PcsI WCGNNNNNNNCGW 1 cut(s) 29
PctI GAATGC 2 cut(s) 1829, 1945
PdiI GCCGGC 3 cut(s) 108, 2093, 2791
PdmI GAANNNNTTC 1 cut(s) 709
PfeI GAWTC 9 cut(s) 710, 996, 1831, 1880, 2487, 2948, 3095, 3120, 3265
PfoI TCCNGGA 1 cut(s) 3308
PinAI ACCGGT 1 cut(s) 3297
PleI GAGTC 7 cut(s) 67, 310, 1675, 2669, 3015, 3036, 3313
PmeI GTTTAAAC 1 cut(s) 2221
PpsI GAGTC 7 cut(s) 67, 310, 1675, 2669, 3015, 3036, 3313
PscI ACATGT 1 cut(s) 1311
PshBI ATTAAT 2 cut(s) 1596, 1704
Psp124BI GAGCTC 1 cut(s) 41
Psp6I CCWGG 5 cut(s) 630, 1443, 3058, 3241, 3308
PspEI GGTNACC 2 cut(s) 318, 2621
PspFI CCCAGC 3 cut(s) 839, 1342, 1628
PspGI CCWGG 5 cut(s) 630, 1443, 3058, 3241, 3308
PspN4I GGNNCC 3 cut(s) 1486, 2175, 2454
PspPI GGNCC 2 cut(s) 656, 2173
PstI CTGCAG 3 cut(s) 2103, 2919, 3019
PstNI CAGNNNCTG 2 cut(s) 773, 2192
PsuI RGATCY 2 cut(s) 1634, 2452
PvuII CAGCTG 1 cut(s) 3254
RsaI GTAC 4 cut(s) 1193, 2156, 2510, 3323
RsaNI GTAC 4 cut(s) 1192, 2155, 2509, 3322
RseI CAYNNNNRTG 5 cut(s) 756, 918, 1427, 1938, 3113
SacI GAGCTC 1 cut(s) 41
Sau3AI GATC 7 cut(s) 289, 565, 1300, 1634, 2369, 2452, 2644
Sau96I GGNCC 2 cut(s) 656, 2173
SchI GAGTC 7 cut(s) 68, 311, 1676, 2670, 3016, 3037, 3314
ScrFI CCNGG 8 cut(s) 632, 655, 1445, 2295, 2627, 3060, 3243, 3310
SduI GDGCHC 3 cut(s) 41, 516, 2632
SfcI CTRYAG 3 cut(s) 2099, 2915, 3015
SinI GGWCC 1 cut(s) 2173
SmiMI CAYNNNNRTG 5 cut(s) 756, 918, 1427, 1938, 3113
SmlI CTYRAG 6 cut(s) 1757, 2123, 2330, 2651, 3137, 3418
SmoI CTYRAG 6 cut(s) 1757, 2123, 2330, 2651, 3137, 3418
SphI GCATGC 1 cut(s) 917
SstI GAGCTC 1 cut(s) 41
StyD4I CCNGG 8 cut(s) 630, 653, 1443, 2293, 2625, 3058, 3241, 3308
StyI CCWWGG 3 cut(s) 132, 1488, 3114
TaaI ACNGT 8 cut(s) 82, 298, 314, 332, 456, 1103, 2282, 2684
TaqI TCGA 7 cut(s) 23, 84, 183, 552, 1883, 2963, 3207
TatI WGTACW 2 cut(s) 1191, 3321
TauI GCSGC 4 cut(s) 106, 127, 159, 1331
TfiI GAWTC 9 cut(s) 710, 996, 1831, 1880, 2487, 2948, 3095, 3120, 3265
TscAI CASTG 7 cut(s) 337, 623, 1033, 1338, 1521, 1617, 2497
TseFI GTSAC 4 cut(s) 318, 1602, 2621, 2754
Tsp45I GTSAC 4 cut(s) 318, 1602, 2621, 2754
TspGWI ACGGA 4 cut(s) 9, 296, 1469, 2606
TspRI CASTG 7 cut(s) 337, 623, 1033, 1338, 1521, 1617, 2497
Vha464I CTTAAG 2 cut(s) 2123, 2651
VneI GTGCAC 1 cut(s) 512
VpaK11BI GGWCC 1 cut(s) 2173
VspI ATTAAT 2 cut(s) 1596, 1704
XapI RAATTY 7 cut(s) 696, 1553, 1922, 2402, 2743, 3182, 3363
XceI RCATGY 4 cut(s) 917, 1315, 1385, 2302
XmiI GTMKAC 2 cut(s) 943, 1645
XmnI GAANNNNTTC 1 cut(s) 709
Zsp2I ATGCAT 1 cut(s) 2170
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.