MD03G1219500.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0012454
Physical Location & Seq
Forward (+)
30415472 .. 30417100
1629 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1219500.v1.1.491

Sequence Viewer

Length: 255 bp
ATGAGTACGCTTGATGTGACCATGGGAGATGGCGGCGGCGCCCAACCGGAATCTCGATGGAAGCAAATGGGGCATGGGGAGAAGGAGGCGTCCGTGCACGAAGAAATGAAGAGAATGCAGAAGTTGCCTGCAAACAGCACTTACGTCACTCACCGGTTGCGGGTTCTCAACAAAATTCTCCAGCTTTTGTCCATTCAGAGAACCGCATCACAAGAACAGGAGTTGGAGTTGCTTTTCGCCGGGCTGTCTATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

85

Amino Acids

9.45

Weight (kDa)

6.83

Isoelectric Point (pI)

61.35

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012454)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G13677 AT3G13677 AT3G13677
fragaria_vesca FvH4_3g20900 FvH4_3g20900
malus_domestica MD03G1219500.v1.1
prunus_persica Prupe.4G189600_v2.0.a1 Prupe.4G189600_v2.0.a1
pyrus_communis pycom03g16880
rosa_chinensis RchiOBHm_Chr5g0035321
rosa_laevigata RLG00000033617
rosa_multiflora Rmu_sc0007948.1_g000013 Rmu_sc0008116.1_g000001
rosa_roxburghii Rroxscaffold_1G00045080
rosa_rugosa Rorug05G0151100
rosa_samantha Rh5AG244300 Rh5BG243800 Rh5CG275600
rosa_wichuraiana Rw5G022310

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 38
AciI CCGC 4 cut(s) 33, 36, 160, 204
AcsI RAATTY 1 cut(s) 174
AcyI GRCGYC 2 cut(s) 39, 89
AfaI GTAC 1 cut(s) 7
AfiI CCNNNNNNNGG 1 cut(s) 160
AgeI ACCGGT 1 cut(s) 153
AluBI AGCT 1 cut(s) 184
AluI AGCT 1 cut(s) 184
Alw21I GWGCWC 1 cut(s) 99
Alw44I GTGCAC 1 cut(s) 95
ApaLI GTGCAC 1 cut(s) 95
ApoI RAATTY 1 cut(s) 174
AsiGI ACCGGT 1 cut(s) 153
AspLEI GCGC 1 cut(s) 41
AsuC2I CCSGG 1 cut(s) 241
AsuHPI GGTGA 1 cut(s) 143
BaeGI GKGCMC 1 cut(s) 99
BanI GGYRCC 1 cut(s) 38
Bbv12I GWGCWC 1 cut(s) 99
BccI CCATC 2 cut(s) 23, 51
BcnI CCSGG 1 cut(s) 241
BfoI RGCGCY 1 cut(s) 42
BisI GCNGC 2 cut(s) 34, 37
BlsI GCNGC 2 cut(s) 35, 38
Bme1390I CCNGG 1 cut(s) 241
BmiI GGNNCC 1 cut(s) 40
BmrFI CCNGG 1 cut(s) 241
BmsI GCATC 1 cut(s) 215
BpmI CTGGAG 1 cut(s) 164
BpuMI CCSGG 1 cut(s) 241
BsaHI GRCGYC 2 cut(s) 39, 89
BsaJI CCNNGG 1 cut(s) 21
BsaWI WCCGGW 2 cut(s) 46, 153
BsaXI ACNNNNNCTCC 2 cut(s) 212, 242
Bsc4I CCNNNNNNNGG 1 cut(s) 160
Bse118I RCCGGY 1 cut(s) 153
BseDI CCNNGG 1 cut(s) 21
BseLI CCNNNNNNNGG 1 cut(s) 160
BseSI GKGCMC 1 cut(s) 99
BshNI GGYRCC 1 cut(s) 38
BshTI ACCGGT 1 cut(s) 153
BsiHKAI GWGCWC 1 cut(s) 99
BsiSI CCGG 3 cut(s) 47, 154, 240
BslI CCNNNNNNNGG 1 cut(s) 160
BsmI GAATGC 1 cut(s) 120
Bsp1286I GDGCHC 1 cut(s) 99
Bsp19I CCATGG 1 cut(s) 21
BspACI CCGC 4 cut(s) 33, 36, 160, 204
BspLI GGNNCC 1 cut(s) 40
BspT107I GGYRCC 1 cut(s) 38
BsrFI RCCGGY 1 cut(s) 153
BssAI RCCGGY 1 cut(s) 153
BssECI CCNNGG 1 cut(s) 21
BssNI GRCGYC 2 cut(s) 39, 89
BssT1I CCWWGG 1 cut(s) 21
Bst6I CTCTTC 1 cut(s) 104
BstACI GRCGYC 2 cut(s) 39, 89
BstAPI GCANNNNNTGC 1 cut(s) 124
BstC8I GCNNGC 1 cut(s) 129
BstDSI CCRYGG 1 cut(s) 21
BstH2I RGCGCY 1 cut(s) 42
BstHHI GCGC 1 cut(s) 41
BstMWI GCNNNNNNNGC 2 cut(s) 70, 124
BstSCI CCNGG 1 cut(s) 239
BstSLI GKGCMC 1 cut(s) 99
BtgI CCRYGG 1 cut(s) 21
Cac8I GCNNGC 1 cut(s) 129
CfoI GCGC 1 cut(s) 41
Cfr10I RCCGGY 1 cut(s) 153
CseI GACGC 1 cut(s) 78
Csp6I GTAC 1 cut(s) 6
CspAI ACCGGT 1 cut(s) 153
CviAII CATG 2 cut(s) 22, 74
CviJI RGCY 2 cut(s) 184, 244
CviKI_1 RGCY 2 cut(s) 184, 244
CviQI GTAC 1 cut(s) 6
DinI GGCGCC 1 cut(s) 40
Eam1104I CTCTTC 1 cut(s) 104
EarI CTCTTC 1 cut(s) 104
Eco130I CCWWGG 1 cut(s) 21
EcoT14I CCWWGG 1 cut(s) 21
EgeI GGCGCC 1 cut(s) 40
EheI GGCGCC 1 cut(s) 40
ErhI CCWWGG 1 cut(s) 21
FaeI CATG 2 cut(s) 25, 77
FaiI YATR 3 cut(s) 23, 75, 251
FatI CATG 2 cut(s) 21, 73
FauI CCCGC 1 cut(s) 153
Fnu4HI GCNGC 2 cut(s) 34, 37
Fsp4HI GCNGC 2 cut(s) 34, 37
GlaI GCGC 1 cut(s) 40
GluI GCNGC 2 cut(s) 34, 37
GsuI CTGGAG 1 cut(s) 164
HaeII RGCGCY 1 cut(s) 42
HapII CCGG 3 cut(s) 47, 154, 240
HgaI GACGC 1 cut(s) 78
HhaI GCGC 1 cut(s) 41
Hin1I GRCGYC 2 cut(s) 39, 89
Hin1II CATG 2 cut(s) 25, 77
Hin6I GCGC 1 cut(s) 39
HinP1I GCGC 1 cut(s) 39
HinfI GANTC 1 cut(s) 50
HpaII CCGG 3 cut(s) 47, 154, 240
HphI GGTGA 1 cut(s) 143
Hpy166II GTNNAC 1 cut(s) 97
Hpy188I TCNGA 1 cut(s) 198
Hpy188III TCNNGA 1 cut(s) 54
Hpy8I GTNNAC 1 cut(s) 97
HpyAV CCTTC 1 cut(s) 76
HpyCH4IV ACGT 1 cut(s) 144
HpyCH4V TGCA 3 cut(s) 97, 118, 131
HpyF10VI GCNNNNNNNGC 2 cut(s) 70, 124
HpySE526I ACGT 1 cut(s) 144
Hsp92I GRCGYC 2 cut(s) 39, 89
Hsp92II CATG 2 cut(s) 25, 77
HspAI GCGC 1 cut(s) 39
KasI GGCGCC 1 cut(s) 38
LpnPI CCDG 5 cut(s) 60, 141, 167, 194, 203
LweI GCATC 1 cut(s) 215
MaeII ACGT 1 cut(s) 144
MaeIII GTNAC 2 cut(s) 16, 145
MboII GAAGA 2 cut(s) 113, 121
MhlI GDGCHC 1 cut(s) 99
MluCI AATT 1 cut(s) 174
Mly113I GGCGCC 1 cut(s) 39
MmeI TCCRAC 1 cut(s) 204
MnlI CCTC 1 cut(s) 79
MspI CCGG 3 cut(s) 47, 154, 240
MspR9I CCNGG 1 cut(s) 241
Mva1269I GAATGC 1 cut(s) 120
MwoI GCNNNNNNNGC 2 cut(s) 70, 124
NarI GGCGCC 1 cut(s) 39
NciI CCSGG 1 cut(s) 241
NcoI CCATGG 1 cut(s) 21
NlaIII CATG 2 cut(s) 25, 77
NlaIV GGNNCC 1 cut(s) 40
NmuCI GTSAC 2 cut(s) 16, 145
PctI GAATGC 1 cut(s) 120
PfeI GAWTC 1 cut(s) 50
PinAI ACCGGT 1 cut(s) 153
PkrI GCNGC 2 cut(s) 35, 38
PluTI GGCGCC 1 cut(s) 42
PspN4I GGNNCC 1 cut(s) 40
RsaI GTAC 1 cut(s) 7
RsaNI GTAC 1 cut(s) 6
SatI GCNGC 2 cut(s) 34, 37
ScrFI CCNGG 1 cut(s) 241
SduI GDGCHC 1 cut(s) 99
SetI ASST 2 cut(s) 147, 186
SfaNI GCATC 1 cut(s) 215
SfoI GGCGCC 1 cut(s) 40
Sse9I AATT 1 cut(s) 174
SsiI CCGC 4 cut(s) 33, 36, 160, 204
SspDI GGCGCC 1 cut(s) 38
StyD4I CCNGG 1 cut(s) 239
StyI CCWWGG 1 cut(s) 21
TaiI ACGT 1 cut(s) 147
TaqI TCGA 1 cut(s) 55
TasI AATT 1 cut(s) 174
TauI GCSGC 2 cut(s) 36, 39
TfiI GAWTC 1 cut(s) 50
TseFI GTSAC 2 cut(s) 16, 145
Tsp45I GTSAC 2 cut(s) 16, 145
TspDTI ATGAA 1 cut(s) 122
TspGWI ACGGA 1 cut(s) 82
VneI GTGCAC 1 cut(s) 95
XapI RAATTY 1 cut(s) 174
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.