MD03G1235600.v1.1

Protein of unknown function (DUF3082)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0007406
Physical Location & Seq
Forward (+)
32152972 .. 32155446
2475 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1235600.v1.1.491

Sequence Viewer

Length: 870 bp
ATGTTGCAGACCCAACACCTTCTCCGTTCCAATTTCCCCAAATTTCTCTCTCATCCCCATACCCACTTTGTCTCTCCTCTCTATCTCTCTCCCCCTCCACTCAATTTTTTCCACAAACCCACCAATCTCTGCCCTGTCTCAGCCCATTCCAGACCCGGCCTCGAACAATGTCTAGCCGAAGCTCCAGAACCGACGACCGAAACTCCATACACACCCTTGGAATTTGCTCCAGACGGTCCCGAAGAATTGCCACCGTCTTCTTCTCCAGTGTTTGCCACCAATGATGACCCTTCATCTCTCCAAGTGGCCACCAGTGTTCTTCTCACCGGGGCCATCACTGTCTTCCTCTTTCGCTCCCTGCGACGCCGCGCCAAGCGTGCTAAAGAACTGAAATTTAGGTCATCTGGAATGAAGAAGTCCTTGAAGGATGAGGCTTTGGATAGCTTGAAAGCACTTTCTACAGGTTCTGTTGAAGCAAAGGGTCCACCCTCACCTGTTCAGGCCTTGCTGGGTGGAATATCAGCCGGTGTCATTGCGCTTATTCTTTACAAGTTCACTACTACAATTGAAGCATCTCTCAACCGCCAAACTATTTCTGATAATTTCTCGGTTCGTCAGATTACAATAACCATCAGGACCATTATAAATGGGTTGTGCTACCTTGCAACATTTGTGTTCGGCATCAATGCTGTGGGTTTAGTCCTTTATGCTGGTCAGCTCGCCATCAACTCCATCATGGAAGATACAAATGAAAAAACTCAGACTCAAGGTAAGGATCAGTCAGGCTCATTCAATTCAAAGGTTGAGAGCCCCGCAAATAGTGGTGAGAACAGCGATAGGGATGATCAAAGTTCAGATGGAACACAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3082

Protein Analysis

290

Amino Acids

31.29

Weight (kDa)

6.6

Isoelectric Point (pI)

50.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF3082 PF11282 163 - 240 1.2e-27 Protein of unknown function (DUF3082)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 644
AccII CGCG 1 cut(s) 369
AciI CCGC 3 cut(s) 367, 583, 813
AclWI GGATC 1 cut(s) 783
AcoI YGGCCR 1 cut(s) 306
AcsI RAATTY 3 cut(s) 41, 221, 392
AcyI GRCGYC 1 cut(s) 364
AgsI TTSAA 6 cut(s) 424, 448, 473, 569, 793, 798
AluBI AGCT 3 cut(s) 182, 444, 718
AluI AGCT 3 cut(s) 182, 444, 718
Alw26I GTCTC 2 cut(s) 76, 142
AlwI GGATC 1 cut(s) 783
AlwNI CAGNNNCTG 1 cut(s) 467
AoxI GGCC 4 cut(s) 157, 306, 330, 501
ApoI RAATTY 3 cut(s) 41, 221, 392
AspLEI GCGC 2 cut(s) 371, 538
AspS9I GGNCC 4 cut(s) 236, 330, 482, 636
AsuC2I CCSGG 2 cut(s) 156, 328
AsuHPI GGTGA 3 cut(s) 316, 483, 836
AvaII GGWCC 3 cut(s) 236, 482, 636
BalI TGGCCA 1 cut(s) 308
BanII GRGCYC 1 cut(s) 812
BbsI GAAGAC 2 cut(s) 249, 334
BccI CCATC 5 cut(s) 341, 638, 731, 740, 851
BclI TGATCA 1 cut(s) 844
BcnI CCSGG 2 cut(s) 156, 328
BcoDI GTCTC 2 cut(s) 76, 142
BfaI CTAG 1 cut(s) 173
BfmI CTRYAG 1 cut(s) 459
BisI GCNGC 1 cut(s) 367
BlsI GCNGC 1 cut(s) 368
Bme1390I CCNGG 2 cut(s) 156, 328
Bme18I GGWCC 3 cut(s) 236, 482, 636
BmgT120I GGNCC 4 cut(s) 236, 330, 482, 636
BmiI GGNNCC 3 cut(s) 238, 331, 483
BmrFI CCNGG 2 cut(s) 156, 328
BmsI GCATC 2 cut(s) 581, 690
BpiI GAAGAC 2 cut(s) 249, 334
BpmI CTGGAG 3 cut(s) 168, 213, 249
BpuEI CTTGAG 1 cut(s) 750
BpuMI CCSGG 2 cut(s) 156, 328
BsaHI GRCGYC 1 cut(s) 364
BsaJI CCNNGG 2 cut(s) 216, 327
BsaXI ACNNNNNCTCC 4 cut(s) 6, 36, 187, 217
Bse118I RCCGGY 1 cut(s) 524
Bse1I ACTGG 2 cut(s) 266, 312
Bse3DI GCAATG 1 cut(s) 531
BseDI CCNNGG 2 cut(s) 216, 327
BseGI GGATG 3 cut(s) 52, 433, 847
BseMI GCAATG 1 cut(s) 531
BseMII CTCAG 2 cut(s) 153, 773
BseNI ACTGG 2 cut(s) 266, 312
BseRI GAGGAG 1 cut(s) 66
BseYI CCCAGC 1 cut(s) 508
Bsh1236I CGCG 1 cut(s) 369
Bsh1285I CGRYCG 1 cut(s) 198
BshFI GGCC 4 cut(s) 159, 308, 332, 503
BsiEI CGRYCG 1 cut(s) 198
BsiSI CCGG 3 cut(s) 156, 327, 525
BslFI GGGAC 1 cut(s) 222
BsmAI GTCTC 2 cut(s) 76, 142
BsmFI GGGAC 1 cut(s) 222
BsnI GGCC 4 cut(s) 159, 308, 332, 503
Bsp1286I GDGCHC 1 cut(s) 812
Bsp143I GATC 2 cut(s) 775, 844
BspACI CCGC 3 cut(s) 367, 583, 813
BspANI GGCC 4 cut(s) 159, 308, 332, 503
BspCNI CTCAG 2 cut(s) 152, 772
BspFNI CGCG 1 cut(s) 369
BspLI GGNNCC 3 cut(s) 238, 331, 483
BspPI GGATC 1 cut(s) 783
BsrDI GCAATG 1 cut(s) 531
BsrFI RCCGGY 1 cut(s) 524
BsrI ACTGG 2 cut(s) 266, 312
BssAI RCCGGY 1 cut(s) 524
BssECI CCNNGG 2 cut(s) 216, 327
BssMI GATC 2 cut(s) 775, 844
BssNI GRCGYC 1 cut(s) 364
BssT1I CCWWGG 1 cut(s) 216
Bst4CI ACNGT 3 cut(s) 236, 255, 340
BstACI GRCGYC 1 cut(s) 364
BstC8I GCNNGC 2 cut(s) 378, 720
BstDEI CTNAG 2 cut(s) 139, 759
BstF5I GGATG 3 cut(s) 52, 433, 847
BstFNI CGCG 1 cut(s) 369
BstHHI GCGC 2 cut(s) 371, 538
BstKTI GATC 2 cut(s) 778, 847
BstMAI GTCTC 2 cut(s) 76, 142
BstMBI GATC 2 cut(s) 775, 844
BstMCI CGRYCG 1 cut(s) 198
BstMWI GCNNNNNNNGC 1 cut(s) 377
BstSCI CCNGG 2 cut(s) 154, 326
BstSFI CTRYAG 1 cut(s) 459
BstUI CGCG 1 cut(s) 369
BstV2I GAAGAC 2 cut(s) 249, 334
BsuRI GGCC 4 cut(s) 159, 308, 332, 503
BtsCI GGATG 3 cut(s) 52, 433, 847
BtsIMutI CAGTG 3 cut(s) 273, 319, 336
Cac8I GCNNGC 2 cut(s) 378, 720
CaiI CAGNNNCTG 1 cut(s) 467
CfoI GCGC 2 cut(s) 371, 538
Cfr10I RCCGGY 1 cut(s) 524
Cfr13I GGNCC 4 cut(s) 236, 330, 482, 636
CseI GACGC 1 cut(s) 372
CviAII CATG 1 cut(s) 736
DdeI CTNAG 2 cut(s) 139, 759
DpnI GATC 2 cut(s) 777, 846
DpnII GATC 2 cut(s) 775, 844
EaeI YGGCCR 1 cut(s) 306
Eco130I CCWWGG 1 cut(s) 216
Eco147I AGGCCT 1 cut(s) 503
Eco24I GRGCYC 1 cut(s) 812
Eco47I GGWCC 3 cut(s) 236, 482, 636
EcoT14I CCWWGG 1 cut(s) 216
EcoT38I GRGCYC 1 cut(s) 812
ErhI CCWWGG 1 cut(s) 216
FaeI CATG 1 cut(s) 739
FaiI YATR 5 cut(s) 60, 208, 644, 708, 737
FalI AAGNNNNNCTT 2 cut(s) 404, 436
FaqI GGGAC 1 cut(s) 222
FatI CATG 1 cut(s) 735
FauI CCCGC 1 cut(s) 820
FbaI TGATCA 1 cut(s) 844
Fnu4HI GCNGC 1 cut(s) 367
FokI GGATG 3 cut(s) 39, 440, 854
FriOI GRGCYC 1 cut(s) 812
Fsp4HI GCNGC 1 cut(s) 367
FspBI CTAG 1 cut(s) 173
GlaI GCGC 2 cut(s) 370, 537
GluI GCNGC 1 cut(s) 367
GsaI CCCAGC 1 cut(s) 512
GsuI CTGGAG 3 cut(s) 168, 213, 249
HaeIII GGCC 4 cut(s) 159, 308, 332, 503
HapII CCGG 3 cut(s) 156, 327, 525
HgaI GACGC 1 cut(s) 372
HhaI GCGC 2 cut(s) 371, 538
Hin1I GRCGYC 1 cut(s) 364
Hin1II CATG 1 cut(s) 739
Hin6I GCGC 2 cut(s) 369, 536
HinP1I GCGC 2 cut(s) 369, 536
HinfI GANTC 1 cut(s) 763
HpaII CCGG 3 cut(s) 156, 327, 525
HphI GGTGA 3 cut(s) 316, 483, 836
Hpy166II GTNNAC 2 cut(s) 485, 555
Hpy188I TCNGA 4 cut(s) 598, 618, 762, 856
Hpy188III TCNNGA 6 cut(s) 150, 185, 230, 239, 405, 634
Hpy8I GTNNAC 2 cut(s) 485, 555
Hpy99I CGWCG 2 cut(s) 196, 366
HpyAV CCTTC 3 cut(s) 29, 300, 418
HpyCH4III ACNGT 3 cut(s) 236, 255, 340
HpyCH4V TGCA 2 cut(s) 7, 665
HpyF10VI GCNNNNNNNGC 1 cut(s) 377
HpyF3I CTNAG 2 cut(s) 139, 759
Hsp92I GRCGYC 1 cut(s) 364
Hsp92II CATG 1 cut(s) 739
HspAI GCGC 2 cut(s) 369, 536
Ksp22I TGATCA 1 cut(s) 844
Kzo9I GATC 2 cut(s) 775, 844
LmnI GCTCC 3 cut(s) 187, 232, 359
LweI GCATC 2 cut(s) 581, 690
MaeI CTAG 1 cut(s) 173
MalI GATC 2 cut(s) 777, 846
MboI GATC 2 cut(s) 775, 844
MboII GAAGA 7 cut(s) 249, 252, 254, 311, 334, 424, 752
MfeI CAATTG 1 cut(s) 564
MhlI GDGCHC 1 cut(s) 812
MlsI TGGCCA 1 cut(s) 308
MluCI AATT 9 cut(s) 31, 41, 103, 221, 245, 392, 564, 601, 793
MluNI TGGCCA 1 cut(s) 308
MlyI GAGTC 1 cut(s) 757
MnlI CCTC 6 cut(s) 87, 105, 170, 356, 424, 499
Mox20I TGGCCA 1 cut(s) 308
MscI TGGCCA 1 cut(s) 308
Msp20I TGGCCA 1 cut(s) 308
MspI CCGG 3 cut(s) 156, 327, 525
MspR9I CCNGG 2 cut(s) 156, 328
MunI CAATTG 1 cut(s) 564
MvnI CGCG 1 cut(s) 369
MwoI GCNNNNNNNGC 1 cut(s) 377
NciI CCSGG 2 cut(s) 156, 328
NdeII GATC 2 cut(s) 775, 844
NlaIII CATG 1 cut(s) 739
NlaIV GGNNCC 3 cut(s) 238, 331, 483
PceI AGGCCT 1 cut(s) 503
PcsI WCGNNNNNNNCGW 1 cut(s) 358
PkrI GCNGC 1 cut(s) 368
PleI GAGTC 1 cut(s) 757
PpsI GAGTC 1 cut(s) 757
PsiI TTATAA 1 cut(s) 644
PspFI CCCAGC 1 cut(s) 508
PspN4I GGNNCC 3 cut(s) 238, 331, 483
PspPI GGNCC 4 cut(s) 236, 330, 482, 636
PstNI CAGNNNCTG 1 cut(s) 467
SatI GCNGC 1 cut(s) 367
Sau3AI GATC 2 cut(s) 775, 844
Sau96I GGNCC 4 cut(s) 236, 330, 482, 636
SchI GAGTC 1 cut(s) 757
ScrFI CCNGG 2 cut(s) 156, 328
SduI GDGCHC 1 cut(s) 812
SfaNI GCATC 2 cut(s) 581, 690
SfcI CTRYAG 1 cut(s) 459
SinI GGWCC 3 cut(s) 236, 482, 636
SmlI CTYRAG 1 cut(s) 765
SmoI CTYRAG 1 cut(s) 765
Sse9I AATT 9 cut(s) 31, 41, 103, 221, 245, 392, 564, 601, 793
SseBI AGGCCT 1 cut(s) 503
SsiI CCGC 3 cut(s) 367, 583, 813
SspMI CTAG 1 cut(s) 173
StuI AGGCCT 1 cut(s) 503
StyD4I CCNGG 2 cut(s) 154, 326
StyI CCWWGG 1 cut(s) 216
TaaI ACNGT 3 cut(s) 236, 255, 340
TaqI TCGA 1 cut(s) 162
TaqII GACCGA 1 cut(s) 212
TasI AATT 9 cut(s) 31, 41, 103, 221, 245, 392, 564, 601, 793
TauI GCSGC 1 cut(s) 369
TscAI CASTG 3 cut(s) 273, 319, 343
TspDTI ATGAA 3 cut(s) 282, 425, 765
TspGWI ACGGA 1 cut(s) 14
TspRI CASTG 3 cut(s) 273, 319, 343
VpaK11BI GGWCC 3 cut(s) 236, 482, 636
XapI RAATTY 3 cut(s) 41, 221, 392
XspI CTAG 1 cut(s) 173
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.