Rh5AG223900

Protein of unknown function (DUF3082)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0007406
Physical Location & Seq
Reverse (-)
26651720 .. 26655486
3767 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG223900.1

Sequence Viewer

Length: 858 bp
ATGTTGCAGACCCAACACCTCCTCCGTTCCAATCTCCCCATCTCTCTCTCTCCTCCCCAAACCCACTTTCTCTTTCCTCCCAATCTCTCCTCCTCACCTCTCCGTTTTCTCCACAAACCCATCAACCACATCTCCCTCTCAGCGCACTCTACTCCCAGACCGGACCAATGGCTCGCCGAAGTTCCAGAACCTTCCGCCGGAACCGGCACCTATACGATTCCAGACGAAGAGGGTCCCATTGAGATTTTGGATTCTGATTCTCCAGTGTTTGCTACCAGTGATGACCCTTCTTTTATCCAAACTGCCACCAGTGTTCTCCTCACTGGAGCCATCACTGTTTTCCTCTTTCGCTCGCTACGACGTCGTGCGAGGCGTGCAAAAGAGCTGAAATTTAGGTCTAATGGAGCGAAGAAGTCGTTGAAGGAGGAGGCTTTGGATAGCTTGAAAGTGATGGCTTCAGGGTCCATTGAATCAAAGACACCTTCACCGGTTCAGGCATTGTTGGGTGGAATATCTGCCGGAGTCATTGCACTTATTCTTTACAAGTTCACTACTACAATTGAGGCATCTCTCAACCGCCAAACGATTTCAGATAATTTTTCAGTTCGTCAGATTACAATAACAATAAGGACCATTATAAATGGGTTGTGCTACCTTGCAACATTTGTTTTTGGCATCAACGCTGTTGGTTTAGTTCTTTATGCTGGCCAGATTTTCATCAACGACGAGTTTAAAAGTAAAGAAACTGAGAATCAAAGTAACGACCAGTCAGGCTCACCGACTTCAATGGTCGAGAGTCCCACAGATAGTGCTGTGGACAGTAGCAAGGGGGATCAAAGTTCAGATGAGCCACAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3082

Protein Analysis

285

Amino Acids

30.98

Weight (kDa)

5.77

Isoelectric Point (pI)

62.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF3082 PF11282 161 - 238 2.7e-27 Protein of unknown function (DUF3082)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 638
AatII GACGTC 1 cut(s) 364
AccB1I GGYRCC 1 cut(s) 206
AciI CCGC 2 cut(s) 195, 577
AclWI GGATC 1 cut(s) 840
AcoI YGGCCR 1 cut(s) 706
AcsI RAATTY 1 cut(s) 389
AcuI CTGAAG 1 cut(s) 441
AcyI GRCGYC 1 cut(s) 361
AfiI CCNNNNNNNGG 1 cut(s) 197
AgeI ACCGGT 1 cut(s) 487
AgsI TTSAA 4 cut(s) 421, 445, 470, 786
AjuI GAANNNNNNNTTGG 2 cut(s) 51, 83
AluBI AGCT 2 cut(s) 385, 441
AluI AGCT 2 cut(s) 385, 441
AlwI GGATC 1 cut(s) 840
AoxI GGCC 1 cut(s) 706
ApoI RAATTY 1 cut(s) 389
AsiGI ACCGGT 1 cut(s) 487
AspLEI GCGC 1 cut(s) 145
AspS9I GGNCC 4 cut(s) 163, 233, 462, 630
AsuHPI GGTGA 3 cut(s) 87, 477, 768
AvaII GGWCC 4 cut(s) 163, 233, 462, 630
BalI TGGCCA 1 cut(s) 708
BanI GGYRCC 1 cut(s) 206
BccI CCATC 4 cut(s) 47, 128, 338, 445
Bme18I GGWCC 4 cut(s) 163, 233, 462, 630
BmgT120I GGNCC 4 cut(s) 163, 233, 462, 630
BmiI GGNNCC 6 cut(s) 202, 208, 234, 235, 328, 463
BmsI GCATC 2 cut(s) 575, 684
BpmI CTGGAG 2 cut(s) 246, 345
BsaBI GATNNNNATC 1 cut(s) 716
BsaHI GRCGYC 1 cut(s) 361
BsaWI WCCGGW 2 cut(s) 160, 487
BsaXI ACNNNNNCTCC 4 cut(s) 6, 36, 116, 146
Bsc4I CCNNNNNNNGG 1 cut(s) 197
Bse118I RCCGGY 2 cut(s) 203, 487
Bse1I ACTGG 5 cut(s) 263, 276, 309, 328, 766
Bse3DI GCAATG 1 cut(s) 525
Bse8I GATNNNNATC 1 cut(s) 716
BseJI GATNNNNATC 1 cut(s) 716
BseLI CCNNNNNNNGG 1 cut(s) 197
BseMI GCAATG 1 cut(s) 525
BseMII CTCAG 2 cut(s) 153, 738
BseNI ACTGG 5 cut(s) 263, 276, 309, 328, 766
BseRI GAGGAG 6 cut(s) 11, 42, 79, 82, 308, 440
BshFI GGCC 1 cut(s) 708
BshNI GGYRCC 1 cut(s) 206
BshTI ACCGGT 1 cut(s) 487
BsiSI CCGG 5 cut(s) 161, 198, 204, 488, 519
BslFI GGGAC 2 cut(s) 219, 783
BslI CCNNNNNNNGG 1 cut(s) 197
BsmFI GGGAC 2 cut(s) 219, 783
BsnI GGCC 1 cut(s) 708
Bsp143I GATC 1 cut(s) 832
BspACI CCGC 2 cut(s) 195, 577
BspANI GGCC 1 cut(s) 708
BspCNI CTCAG 2 cut(s) 152, 739
BspLI GGNNCC 6 cut(s) 202, 208, 234, 235, 328, 463
BspPI GGATC 1 cut(s) 840
BspT107I GGYRCC 1 cut(s) 206
BsrDI GCAATG 1 cut(s) 525
BsrFI RCCGGY 2 cut(s) 203, 487
BsrI ACTGG 5 cut(s) 263, 276, 309, 328, 766
BssAI RCCGGY 2 cut(s) 203, 487
BssMI GATC 1 cut(s) 832
BssNI GRCGYC 1 cut(s) 361
Bst4CI ACNGT 2 cut(s) 337, 821
Bst6I CTCTTC 1 cut(s) 222
BstACI GRCGYC 1 cut(s) 361
BstC8I GCNNGC 4 cut(s) 174, 353, 375, 706
BstDEI CTNAG 2 cut(s) 139, 747
BstHHI GCGC 1 cut(s) 145
BstKTI GATC 1 cut(s) 835
BstMBI GATC 1 cut(s) 832
BstMWI GCNNNNNNNGC 1 cut(s) 374
BsuRI GGCC 1 cut(s) 708
BtsIMutI CAGTG 5 cut(s) 270, 283, 316, 321, 333
Cac8I GCNNGC 4 cut(s) 174, 353, 375, 706
CfoI GCGC 1 cut(s) 145
Cfr10I RCCGGY 2 cut(s) 203, 487
Cfr13I GGNCC 4 cut(s) 163, 233, 462, 630
CspAI ACCGGT 1 cut(s) 487
CviJI RGCY 9 cut(s) 172, 329, 385, 431, 441, 455, 708, 774, 850
CviKI_1 RGCY 9 cut(s) 172, 329, 385, 431, 441, 455, 708, 774, 850
DdeI CTNAG 2 cut(s) 139, 747
DpnI GATC 1 cut(s) 834
DpnII GATC 1 cut(s) 832
DraI TTTAAA 1 cut(s) 733
EaeI YGGCCR 1 cut(s) 706
Eam1104I CTCTTC 1 cut(s) 222
EarI CTCTTC 1 cut(s) 222
EciI GGCGGA 1 cut(s) 184
Eco47I GGWCC 4 cut(s) 163, 233, 462, 630
Eco57I CTGAAG 1 cut(s) 441
EcoO109I RGGNCCY 1 cut(s) 233
FaiI YATR 3 cut(s) 213, 638, 702
FaqI GGGAC 2 cut(s) 219, 783
GlaI GCGC 1 cut(s) 144
GsuI CTGGAG 2 cut(s) 246, 345
HaeIII GGCC 1 cut(s) 708
HapII CCGG 5 cut(s) 161, 198, 204, 488, 519
HhaI GCGC 1 cut(s) 145
Hin1I GRCGYC 1 cut(s) 361
Hin6I GCGC 1 cut(s) 143
HinP1I GCGC 1 cut(s) 143
HinfI GANTC 7 cut(s) 217, 251, 257, 470, 522, 751, 796
HpaII CCGG 5 cut(s) 161, 198, 204, 488, 519
HphI GGTGA 3 cut(s) 87, 477, 768
Hpy166II GTNNAC 2 cut(s) 549, 817
Hpy188I TCNGA 4 cut(s) 256, 592, 612, 844
Hpy188III TCNNGA 3 cut(s) 185, 221, 793
Hpy8I GTNNAC 2 cut(s) 549, 817
Hpy99I CGWCG 3 cut(s) 363, 366, 728
HpyAV CCTTC 4 cut(s) 201, 297, 415, 492
HpyCH4III ACNGT 2 cut(s) 337, 821
HpyCH4IV ACGT 1 cut(s) 361
HpyCH4V TGCA 4 cut(s) 7, 377, 530, 659
HpyF10VI GCNNNNNNNGC 1 cut(s) 374
HpyF3I CTNAG 2 cut(s) 139, 747
HpySE526I ACGT 1 cut(s) 361
Hsp92I GRCGYC 1 cut(s) 361
HspAI GCGC 1 cut(s) 143
KflI GGGWCCC 1 cut(s) 233
Kzo9I GATC 1 cut(s) 832
LmnI GCTCC 2 cut(s) 326, 404
LweI GCATC 2 cut(s) 575, 684
MaeII ACGT 1 cut(s) 361
MaeIII GTNAC 1 cut(s) 758
MalI GATC 1 cut(s) 834
MboI GATC 1 cut(s) 832
MboII GAAGA 2 cut(s) 239, 421
MfeI CAATTG 1 cut(s) 558
MlsI TGGCCA 1 cut(s) 708
MluCI AATT 3 cut(s) 389, 558, 595
MluNI TGGCCA 1 cut(s) 708
MlyI GAGTC 2 cut(s) 531, 805
Mox20I TGGCCA 1 cut(s) 708
MscI TGGCCA 1 cut(s) 708
MseI TTAA 1 cut(s) 732
Msp20I TGGCCA 1 cut(s) 708
MspI CCGG 5 cut(s) 161, 198, 204, 488, 519
MunI CAATTG 1 cut(s) 558
MwoI GCNNNNNNNGC 1 cut(s) 374
NdeII GATC 1 cut(s) 832
NlaIV GGNNCC 6 cut(s) 202, 208, 234, 235, 328, 463
PcsI WCGNNNNNNNCGW 2 cut(s) 355, 370
PfeI GAWTC 5 cut(s) 217, 251, 257, 470, 751
PinAI ACCGGT 1 cut(s) 487
PleI GAGTC 2 cut(s) 530, 804
PpsI GAGTC 2 cut(s) 530, 804
PpuMI RGGWCCY 1 cut(s) 233
PsiI TTATAA 1 cut(s) 638
Psp5II RGGWCCY 1 cut(s) 233
PspN4I GGNNCC 6 cut(s) 202, 208, 234, 235, 328, 463
PspPI GGNCC 4 cut(s) 163, 233, 462, 630
PspPPI RGGWCCY 1 cut(s) 233
SaqAI TTAA 1 cut(s) 732
Sau3AI GATC 1 cut(s) 832
Sau96I GGNCC 4 cut(s) 163, 233, 462, 630
SchI GAGTC 2 cut(s) 531, 805
SfaNI GCATC 2 cut(s) 575, 684
SinI GGWCC 4 cut(s) 163, 233, 462, 630
Sse9I AATT 3 cut(s) 389, 558, 595
SsiI CCGC 2 cut(s) 195, 577
TaaI ACNGT 2 cut(s) 337, 821
TaiI ACGT 1 cut(s) 364
TaqI TCGA 1 cut(s) 792
TasI AATT 3 cut(s) 389, 558, 595
TfiI GAWTC 5 cut(s) 217, 251, 257, 470, 751
Tru1I TTAA 1 cut(s) 732
Tru9I TTAA 1 cut(s) 732
TscAI CASTG 5 cut(s) 270, 283, 316, 328, 340
TspDTI ATGAA 1 cut(s) 706
TspGWI ACGGA 2 cut(s) 14, 92
TspRI CASTG 5 cut(s) 270, 283, 316, 328, 340
VpaK11BI GGWCC 4 cut(s) 163, 233, 462, 630
XapI RAATTY 1 cut(s) 389
XcmI CCANNNNNNNNNTGG 1 cut(s) 244
ZraI GACGTC 1 cut(s) 362
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.