MD03G1256500.v1.1

Guanine nucleotide-binding protein subunit gamma

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0010807
Physical Location & Seq
Reverse (-)
34405485 .. 34407805
2321 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1256500.v1.1.491

Sequence Viewer

Length: 339 bp
ATGGCGTCCGATACGGCGTCGTCTGTGGACGAACAGGCGCAGCAGCTGGTAGCTCCGGTTGGTTCAGTCGGAGGAGGAGGCTCCACTGATAACAGAGGAAGGCATCGGATTCTCGCCGAACTCAGCCGCCTCGAGCAAGAACTCAAATTCTTGGAGGGGGAGTTGGAAGAGCTCGAAAGAACCGAAAATGTTTCAACCATATGTTCAGAGTTGCTGCCCTACATCGAAGGCAGGCCGGATCCTCTACTCCCAGTAACAAATGGACCCGTAAATCTGGTATGGGATCGATGGTTCGAAGGGCCGCAGGATTCACAAAGTTGCAGCTGCGGGATACTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000139 GO:0000166 GO:0001882 GO:0001883 GO:0002376 GO:0003674 GO:0005488 GO:0005525 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005794 GO:0005802 GO:0005834 GO:0005886 GO:0006464 GO:0006497 GO:0006807 GO:0006810 GO:0006950 GO:0006952 GO:0006955 GO:0007275 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009605 GO:0009607 GO:0009620 GO:0009791 GO:0009814 GO:0009817 GO:0009845 GO:0009898 GO:0009914 GO:0009926 GO:0009987 GO:0010541 GO:0010817 GO:0012505 GO:0016020 GO:0017076 GO:0018342 GO:0018345 GO:0019001 GO:0019538 GO:0019897 GO:0019898 GO:0022622 GO:0031090 GO:0031234 GO:0031984 GO:0032501 GO:0032502 GO:0032549 GO:0032550 GO:0032553 GO:0032555 GO:0032561 GO:0032588 GO:0032991 GO:0034645 GO:0035639 GO:0036094 GO:0036211 GO:0042157 GO:0042158 GO:0043167 GO:0043168 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043543 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044425 GO:0044431 GO:0044444 GO:0044446 GO:0044459 GO:0044464 GO:0045087 GO:0048364 GO:0048527 GO:0048528 GO:0048731 GO:0048856 GO:0050832 GO:0050896 GO:0051179 GO:0051234 GO:0051704 GO:0051707 GO:0060918 GO:0065007 GO:0065008 GO:0071704 GO:0071944 GO:0090351 GO:0090696 GO:0097159 GO:0097354 GO:0097367 GO:0098542 GO:0098552 GO:0098562 GO:0098588 GO:0098791 GO:0098796 GO:0098797 GO:0099402 GO:1901265 GO:1901363 GO:1901564 GO:1901566 GO:1901576 GO:1902494 GO:1905360
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
G-gamma

Protein Analysis

113

Amino Acids

12.2

Weight (kDa)

4.29

Isoelectric Point (pI)

61.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
G-gamma PF00631 41 - 109 3.4e-12 GGL domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0010807)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G63420 AT3G63420 AT3G63420
fragaria_vesca FvH4_2g22840
malus_domestica MD03G1256500.v1.1 MD11G1277200.v1.1
prunus_persica Prupe.8G233500_v2.0.a1
pyrus_communis pycom03g20330 pycom11g24520
rosa_chinensis RchiOBHm_Chr6g0289751
rosa_laevigata RLG00000012269
rosa_multiflora Rmu_sc0005640.1_g000016
rosa_roxburghii Rroxscaffold_7G00177590 Rroxscaffold_7G00178090
rosa_rugosa Rorug06G0206300
rosa_samantha Rh6AG318200 Rh6BG325500 Rh6CG331100 Rh6DG316800
rosa_wichuraiana Rw6G027430

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 3 cut(s) 127, 302, 327
AclWI GGATC 3 cut(s) 233, 246, 291
AcsI RAATTY 1 cut(s) 146
AcyI GRCGYC 2 cut(s) 5, 17
AgsI TTSAA 1 cut(s) 195
AloI GAACNNNNNNTCC 2 cut(s) 275, 307
AluBI AGCT 4 cut(s) 46, 53, 172, 324
AluI AGCT 4 cut(s) 46, 53, 172, 324
Alw21I GWGCWC 1 cut(s) 174
AlwI GGATC 3 cut(s) 233, 246, 291
AlwNI CAGNNNCTG 1 cut(s) 46
Ama87I CYCGRG 1 cut(s) 131
AoxI GGCC 2 cut(s) 233, 299
ApeKI GCWGC 5 cut(s) 40, 43, 214, 321, 324
ApoI RAATTY 1 cut(s) 146
AspLEI GCGC 1 cut(s) 40
AspS9I GGNCC 2 cut(s) 263, 299
AsuII TTCGAA 1 cut(s) 294
AvaI CYCGRG 1 cut(s) 131
AvaII GGWCC 1 cut(s) 263
BamHI GGATCC 1 cut(s) 238
BanII GRGCYC 1 cut(s) 174
Bbv12I GWGCWC 1 cut(s) 174
BbvI GCAGC 5 cut(s) 52, 55, 201, 311, 333
BccI CCATC 1 cut(s) 282
BceAI ACGGC 1 cut(s) 30
BciVI GTATCC 1 cut(s) 324
BfuI GTATCC 1 cut(s) 324
BisI GCNGC 7 cut(s) 41, 44, 127, 215, 302, 322, 325
BlsI GCNGC 7 cut(s) 42, 45, 128, 216, 303, 323, 326
Bme18I GGWCC 1 cut(s) 263
BmeT110I CYCGRG 1 cut(s) 131
BmgT120I GGNCC 2 cut(s) 263, 299
BmiI GGNNCC 3 cut(s) 82, 240, 265
BmrI ACTGGG 1 cut(s) 245
BmsI GCATC 1 cut(s) 112
BmuI ACTGGG 1 cut(s) 245
Bpu14I TTCGAA 1 cut(s) 294
Bsa29I ATCGAT 1 cut(s) 286
BsaHI GRCGYC 2 cut(s) 5, 17
BsaWI WCCGGW 1 cut(s) 55
BsaXI ACNNNNNCTCC 2 cut(s) 146, 176
Bse1I ACTGG 1 cut(s) 251
BseCI ATCGAT 1 cut(s) 286
BseMII CTCAG 1 cut(s) 136
BseNI ACTGG 1 cut(s) 251
BseRI GAGGAG 2 cut(s) 87, 90
BseXI GCAGC 5 cut(s) 52, 55, 201, 311, 333
BshFI GGCC 2 cut(s) 235, 301
BshVI ATCGAT 1 cut(s) 286
BsiHKAI GWGCWC 1 cut(s) 174
BsiHKCI CYCGRG 1 cut(s) 131
BsiSI CCGG 2 cut(s) 56, 236
BsnI GGCC 2 cut(s) 235, 301
BsoBI CYCGRG 1 cut(s) 131
Bsp119I TTCGAA 1 cut(s) 294
Bsp1286I GDGCHC 1 cut(s) 174
Bsp143I GATC 2 cut(s) 238, 283
BspACI CCGC 3 cut(s) 127, 302, 327
BspANI GGCC 2 cut(s) 235, 301
BspCNI CTCAG 1 cut(s) 135
BspDI ATCGAT 1 cut(s) 286
BspLI GGNNCC 3 cut(s) 82, 240, 265
BspPI GGATC 3 cut(s) 233, 246, 291
BspQI GCTCTTC 1 cut(s) 162
BspT104I TTCGAA 1 cut(s) 294
BsrI ACTGG 1 cut(s) 251
BssMI GATC 2 cut(s) 238, 283
BssNI GRCGYC 2 cut(s) 5, 17
Bst6I CTCTTC 1 cut(s) 162
BstACI GRCGYC 2 cut(s) 5, 17
BstBI TTCGAA 1 cut(s) 294
BstC8I GCNNGC 1 cut(s) 233
BstDEI CTNAG 1 cut(s) 122
BstHHI GCGC 1 cut(s) 40
BstKTI GATC 2 cut(s) 241, 286
BstMBI GATC 2 cut(s) 238, 283
BstV1I GCAGC 5 cut(s) 52, 55, 201, 311, 333
BstX2I RGATCY 1 cut(s) 238
BstYI RGATCY 1 cut(s) 238
Bsu15I ATCGAT 1 cut(s) 286
BsuI GTATCC 1 cut(s) 324
BsuRI GGCC 2 cut(s) 235, 301
BsuTUI ATCGAT 1 cut(s) 286
BtsIMutI CAGTG 1 cut(s) 84
Cac8I GCNNGC 1 cut(s) 233
CaiI CAGNNNCTG 1 cut(s) 46
CfoI GCGC 1 cut(s) 40
Cfr13I GGNCC 2 cut(s) 263, 299
ClaI ATCGAT 1 cut(s) 286
CseI GACGC 1 cut(s) 6
CviJI RGCY 8 cut(s) 46, 53, 81, 126, 172, 235, 301, 324
CviKI_1 RGCY 8 cut(s) 46, 53, 81, 126, 172, 235, 301, 324
DdeI CTNAG 1 cut(s) 122
DpnI GATC 2 cut(s) 240, 285
DpnII GATC 2 cut(s) 238, 283
Eam1104I CTCTTC 1 cut(s) 162
EarI CTCTTC 1 cut(s) 162
Ecl136II GAGCTC 1 cut(s) 172
Eco24I GRGCYC 1 cut(s) 174
Eco47I GGWCC 1 cut(s) 263
Eco53kI GAGCTC 1 cut(s) 172
Eco88I CYCGRG 1 cut(s) 131
EcoICRI GAGCTC 1 cut(s) 172
EcoT38I GRGCYC 1 cut(s) 174
FaiI YATR 3 cut(s) 200, 202, 280
FauI CCCGC 1 cut(s) 320
FauNDI CATATG 1 cut(s) 200
Fnu4HI GCNGC 7 cut(s) 41, 44, 127, 215, 302, 322, 325
FriOI GRGCYC 1 cut(s) 174
Fsp4HI GCNGC 7 cut(s) 41, 44, 127, 215, 302, 322, 325
GlaI GCGC 1 cut(s) 39
GluI GCNGC 7 cut(s) 41, 44, 127, 215, 302, 322, 325
HaeIII GGCC 2 cut(s) 235, 301
HapII CCGG 2 cut(s) 56, 236
HgaI GACGC 1 cut(s) 6
HhaI GCGC 1 cut(s) 40
Hin1I GRCGYC 2 cut(s) 5, 17
Hin6I GCGC 1 cut(s) 38
HinP1I GCGC 1 cut(s) 38
HinfI GANTC 2 cut(s) 109, 308
HpaII CCGG 2 cut(s) 56, 236
Hpy166II GTNNAC 1 cut(s) 28
Hpy188I TCNGA 5 cut(s) 10, 71, 108, 208, 338
Hpy8I GTNNAC 1 cut(s) 28
Hpy99I CGWCG 1 cut(s) 22
HpyAV CCTTC 3 cut(s) 93, 221, 290
HpyCH4V TGCA 1 cut(s) 321
HpyF3I CTNAG 1 cut(s) 122
Hsp92I GRCGYC 2 cut(s) 5, 17
HspAI GCGC 1 cut(s) 38
Kzo9I GATC 2 cut(s) 238, 283
LguI GCTCTTC 1 cut(s) 162
LmnI GCTCC 2 cut(s) 58, 86
LpnPI CCDG 8 cut(s) 20, 32, 69, 217, 249, 260, 264, 290
Lsp1109I GCAGC 5 cut(s) 52, 55, 201, 311, 333
LweI GCATC 1 cut(s) 112
MaeIII GTNAC 1 cut(s) 253
MalI GATC 2 cut(s) 240, 285
MboI GATC 2 cut(s) 238, 283
MboII GAAGA 1 cut(s) 179
MflI RGATCY 1 cut(s) 238
MhlI GDGCHC 1 cut(s) 174
MluCI AATT 1 cut(s) 146
MmeI TCCRAC 2 cut(s) 49, 144
MnlI CCTC 7 cut(s) 65, 68, 71, 89, 140, 148, 252
MspA1I CMGCKG 2 cut(s) 46, 324
MspI CCGG 2 cut(s) 56, 236
NdeI CATATG 1 cut(s) 200
NdeII GATC 2 cut(s) 238, 283
NlaIV GGNNCC 3 cut(s) 82, 240, 265
NspV TTCGAA 1 cut(s) 294
PaeR7I CTCGAG 1 cut(s) 131
PciSI GCTCTTC 1 cut(s) 162
PcsI WCGNNNNNNNCGW 1 cut(s) 180
PfeI GAWTC 2 cut(s) 109, 308
PkrI GCNGC 7 cut(s) 42, 45, 128, 216, 303, 323, 326
Psp124BI GAGCTC 1 cut(s) 174
PspN4I GGNNCC 3 cut(s) 82, 240, 265
PspPI GGNCC 2 cut(s) 263, 299
PspXI VCTCGAGB 1 cut(s) 131
PstNI CAGNNNCTG 1 cut(s) 46
PsuI RGATCY 1 cut(s) 238
PvuII CAGCTG 2 cut(s) 46, 324
SacI GAGCTC 1 cut(s) 174
SapI GCTCTTC 1 cut(s) 162
SatI GCNGC 7 cut(s) 41, 44, 127, 215, 302, 322, 325
Sau3AI GATC 2 cut(s) 238, 283
Sau96I GGNCC 2 cut(s) 263, 299
SduI GDGCHC 1 cut(s) 174
SetI ASST 4 cut(s) 48, 55, 174, 326
SfaNI GCATC 1 cut(s) 112
Sfr274I CTCGAG 1 cut(s) 131
SfuI TTCGAA 1 cut(s) 294
SinI GGWCC 1 cut(s) 263
SlaI CTCGAG 1 cut(s) 131
SmlI CTYRAG 1 cut(s) 131
SmoI CTYRAG 1 cut(s) 131
Sse9I AATT 1 cut(s) 146
SsiI CCGC 3 cut(s) 127, 302, 327
SstI GAGCTC 1 cut(s) 174
TaqI TCGA 5 cut(s) 132, 174, 225, 286, 294
TasI AATT 1 cut(s) 146
TauI GCSGC 2 cut(s) 129, 304
TfiI GAWTC 2 cut(s) 109, 308
TscAI CASTG 1 cut(s) 91
TseI GCWGC 5 cut(s) 40, 43, 214, 321, 324
TspRI CASTG 1 cut(s) 91
VpaK11BI GGWCC 1 cut(s) 263
XapI RAATTY 1 cut(s) 146
XhoI CTCGAG 1 cut(s) 131
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.