pycom03g20330

Guanine nucleotide-binding protein subunit gamma

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
OG0010807
Physical Location & Seq
Reverse (-)
20848380 .. 20850648
2269 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom03g20330.1

Sequence Viewer

Length: 345 bp
ATGGCGTCCGATACGGCGTCATCTGTGGACGAACAGGCGCAGCAGCGGGTGCCTCCGGTTGTTTCAGTCGGAGGAGGAGGAGGAGGCTCCACTGATAACAGAGGAAGGCATCGGATTCTCGCCGAACTCAGCCGCCTCGAGCAAGAACTCAAATTCTTGGAGGAGGAGTTGGAAGAGCTCGAAAGAACCGAAAATGTTTCAACCATATGTTCAGAGTTGCTGCCCTACATCGAAGGCAGGCCGGATCCTCTACTCCCAGTAACAAATGGACCTGTAAATCTGGTATGGGATCGATGGTTTGAAGGGCCGCAGGATTCACAAAGTTGCAGCTGCTGGATACTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000139 GO:0000166 GO:0001882 GO:0001883 GO:0002376 GO:0003674 GO:0005488 GO:0005525 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005794 GO:0005802 GO:0005834 GO:0005886 GO:0006464 GO:0006497 GO:0006807 GO:0006810 GO:0006950 GO:0006952 GO:0006955 GO:0007275 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009605 GO:0009607 GO:0009620 GO:0009791 GO:0009814 GO:0009817 GO:0009845 GO:0009898 GO:0009914 GO:0009926 GO:0009987 GO:0010541 GO:0010817 GO:0012505 GO:0016020 GO:0017076 GO:0018342 GO:0018345 GO:0019001 GO:0019538 GO:0019897 GO:0019898 GO:0022622 GO:0031090 GO:0031234 GO:0031984 GO:0032501 GO:0032502 GO:0032549 GO:0032550 GO:0032553 GO:0032555 GO:0032561 GO:0032588 GO:0032991 GO:0034645 GO:0035639 GO:0036094 GO:0036211 GO:0042157 GO:0042158 GO:0043167 GO:0043168 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043543 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044425 GO:0044431 GO:0044444 GO:0044446 GO:0044459 GO:0044464 GO:0045087 GO:0048364 GO:0048527 GO:0048528 GO:0048731 GO:0048856 GO:0050832 GO:0050896 GO:0051179 GO:0051234 GO:0051704 GO:0051707 GO:0060918 GO:0065007 GO:0065008 GO:0071704 GO:0071944 GO:0090351 GO:0090696 GO:0097159 GO:0097354 GO:0097367 GO:0098542 GO:0098552 GO:0098562 GO:0098588 GO:0098791 GO:0098796 GO:0098797 GO:0099402 GO:1901265 GO:1901363 GO:1901564 GO:1901566 GO:1901576 GO:1902494 GO:1905360
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
G-gamma

Protein Analysis

115

Amino Acids

12.62

Weight (kDa)

4.34

Isoelectric Point (pI)

74.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
G-gamma PF00631 43 - 114 3.5e-14 GGL domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0010807)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G63420 AT3G63420 AT3G63420
fragaria_vesca FvH4_2g22840
malus_domestica MD03G1256500.v1.1 MD11G1277200.v1.1
prunus_persica Prupe.8G233500_v2.0.a1
pyrus_communis pycom03g20330 pycom11g24520
rosa_chinensis RchiOBHm_Chr6g0289751
rosa_laevigata RLG00000012269
rosa_multiflora Rmu_sc0005640.1_g000016
rosa_roxburghii Rroxscaffold_7G00177590 Rroxscaffold_7G00178090
rosa_rugosa Rorug06G0206300
rosa_samantha Rh6AG318200 Rh6BG325500 Rh6CG331100 Rh6DG316800
rosa_wichuraiana Rw6G027430

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 49
AciI CCGC 3 cut(s) 46, 133, 308
AclWI GGATC 3 cut(s) 239, 252, 297
AcsI RAATTY 1 cut(s) 152
AcyI GRCGYC 2 cut(s) 5, 17
AgsI TTSAA 2 cut(s) 201, 302
AluBI AGCT 2 cut(s) 178, 330
AluI AGCT 2 cut(s) 178, 330
Alw21I GWGCWC 1 cut(s) 180
AlwI GGATC 3 cut(s) 239, 252, 297
AlwNI CAGNNNCTG 1 cut(s) 333
Ama87I CYCGRG 1 cut(s) 137
AoxI GGCC 2 cut(s) 239, 305
ApeKI GCWGC 5 cut(s) 40, 43, 220, 327, 330
ApoI RAATTY 1 cut(s) 152
AspLEI GCGC 1 cut(s) 40
AspS9I GGNCC 2 cut(s) 269, 305
AvaI CYCGRG 1 cut(s) 137
AvaII GGWCC 1 cut(s) 269
BamHI GGATCC 1 cut(s) 244
BanI GGYRCC 1 cut(s) 49
BanII GRGCYC 1 cut(s) 180
Bbv12I GWGCWC 1 cut(s) 180
BbvI GCAGC 5 cut(s) 52, 55, 207, 317, 339
BccI CCATC 1 cut(s) 288
BceAI ACGGC 1 cut(s) 30
BciVI GTATCC 1 cut(s) 330
BfuI GTATCC 1 cut(s) 330
BisI GCNGC 7 cut(s) 41, 44, 133, 221, 308, 328, 331
BlsI GCNGC 7 cut(s) 42, 45, 134, 222, 309, 329, 332
Bme18I GGWCC 1 cut(s) 269
BmeT110I CYCGRG 1 cut(s) 137
BmgT120I GGNCC 2 cut(s) 269, 305
BmiI GGNNCC 3 cut(s) 51, 88, 246
BmrI ACTGGG 1 cut(s) 251
BmsI GCATC 1 cut(s) 118
BmuI ACTGGG 1 cut(s) 251
Bsa29I ATCGAT 1 cut(s) 292
BsaHI GRCGYC 2 cut(s) 5, 17
BsaWI WCCGGW 1 cut(s) 55
BsaXI ACNNNNNCTCC 2 cut(s) 152, 182
Bse1I ACTGG 1 cut(s) 257
BseCI ATCGAT 1 cut(s) 292
BseMII CTCAG 1 cut(s) 142
BseNI ACTGG 1 cut(s) 257
BseRI GAGGAG 6 cut(s) 87, 90, 93, 96, 176, 179
BseXI GCAGC 5 cut(s) 52, 55, 207, 317, 339
BshFI GGCC 2 cut(s) 241, 307
BshNI GGYRCC 1 cut(s) 49
BshVI ATCGAT 1 cut(s) 292
BsiHKAI GWGCWC 1 cut(s) 180
BsiHKCI CYCGRG 1 cut(s) 137
BsiSI CCGG 2 cut(s) 56, 242
BsnI GGCC 2 cut(s) 241, 307
BsoBI CYCGRG 1 cut(s) 137
Bsp1286I GDGCHC 1 cut(s) 180
Bsp143I GATC 2 cut(s) 244, 289
BspACI CCGC 3 cut(s) 46, 133, 308
BspANI GGCC 2 cut(s) 241, 307
BspCNI CTCAG 1 cut(s) 141
BspDI ATCGAT 1 cut(s) 292
BspLI GGNNCC 3 cut(s) 51, 88, 246
BspPI GGATC 3 cut(s) 239, 252, 297
BspQI GCTCTTC 1 cut(s) 168
BspT107I GGYRCC 1 cut(s) 49
BsrI ACTGG 1 cut(s) 257
BssMI GATC 2 cut(s) 244, 289
BssNI GRCGYC 2 cut(s) 5, 17
Bst6I CTCTTC 1 cut(s) 168
BstACI GRCGYC 2 cut(s) 5, 17
BstAPI GCANNNNNTGC 1 cut(s) 49
BstC8I GCNNGC 1 cut(s) 239
BstDEI CTNAG 1 cut(s) 128
BstHHI GCGC 1 cut(s) 40
BstKTI GATC 2 cut(s) 247, 292
BstMBI GATC 2 cut(s) 244, 289
BstMWI GCNNNNNNNGC 1 cut(s) 49
BstV1I GCAGC 5 cut(s) 52, 55, 207, 317, 339
BstX2I RGATCY 1 cut(s) 244
BstYI RGATCY 1 cut(s) 244
Bsu15I ATCGAT 1 cut(s) 292
BsuI GTATCC 1 cut(s) 330
BsuRI GGCC 2 cut(s) 241, 307
BsuTUI ATCGAT 1 cut(s) 292
BtsIMutI CAGTG 1 cut(s) 90
Cac8I GCNNGC 1 cut(s) 239
CaiI CAGNNNCTG 1 cut(s) 333
CfoI GCGC 1 cut(s) 40
Cfr13I GGNCC 2 cut(s) 269, 305
ClaI ATCGAT 1 cut(s) 292
CseI GACGC 1 cut(s) 6
CviJI RGCY 6 cut(s) 87, 132, 178, 241, 307, 330
CviKI_1 RGCY 6 cut(s) 87, 132, 178, 241, 307, 330
DdeI CTNAG 1 cut(s) 128
DpnI GATC 2 cut(s) 246, 291
DpnII GATC 2 cut(s) 244, 289
Eam1104I CTCTTC 1 cut(s) 168
EarI CTCTTC 1 cut(s) 168
Ecl136II GAGCTC 1 cut(s) 178
Eco24I GRGCYC 1 cut(s) 180
Eco47I GGWCC 1 cut(s) 269
Eco53kI GAGCTC 1 cut(s) 178
Eco88I CYCGRG 1 cut(s) 137
EcoICRI GAGCTC 1 cut(s) 178
EcoT38I GRGCYC 1 cut(s) 180
FaiI YATR 3 cut(s) 206, 208, 286
FauI CCCGC 1 cut(s) 39
FauNDI CATATG 1 cut(s) 206
Fnu4HI GCNGC 7 cut(s) 41, 44, 133, 221, 308, 328, 331
FriOI GRGCYC 1 cut(s) 180
Fsp4HI GCNGC 7 cut(s) 41, 44, 133, 221, 308, 328, 331
GlaI GCGC 1 cut(s) 39
GluI GCNGC 7 cut(s) 41, 44, 133, 221, 308, 328, 331
HaeIII GGCC 2 cut(s) 241, 307
HapII CCGG 2 cut(s) 56, 242
HgaI GACGC 1 cut(s) 6
HhaI GCGC 1 cut(s) 40
Hin1I GRCGYC 2 cut(s) 5, 17
Hin6I GCGC 1 cut(s) 38
HinP1I GCGC 1 cut(s) 38
HinfI GANTC 2 cut(s) 115, 314
HpaII CCGG 2 cut(s) 56, 242
Hpy166II GTNNAC 1 cut(s) 28
Hpy188I TCNGA 5 cut(s) 10, 71, 114, 214, 344
Hpy8I GTNNAC 1 cut(s) 28
HpyAV CCTTC 3 cut(s) 99, 227, 296
HpyCH4V TGCA 1 cut(s) 327
HpyF10VI GCNNNNNNNGC 1 cut(s) 49
HpyF3I CTNAG 1 cut(s) 128
Hsp92I GRCGYC 2 cut(s) 5, 17
HspAI GCGC 1 cut(s) 38
Kzo9I GATC 2 cut(s) 244, 289
LguI GCTCTTC 1 cut(s) 168
LmnI GCTCC 1 cut(s) 92
LpnPI CCDG 9 cut(s) 20, 69, 223, 255, 266, 270, 285, 296, 319
Lsp1109I GCAGC 5 cut(s) 52, 55, 207, 317, 339
LweI GCATC 1 cut(s) 118
MaeIII GTNAC 1 cut(s) 259
MalI GATC 2 cut(s) 246, 291
MboI GATC 2 cut(s) 244, 289
MboII GAAGA 1 cut(s) 185
MflI RGATCY 1 cut(s) 244
MhlI GDGCHC 1 cut(s) 180
MluCI AATT 1 cut(s) 152
MmeI TCCRAC 2 cut(s) 49, 150
MspA1I CMGCKG 2 cut(s) 46, 330
MspI CCGG 2 cut(s) 56, 242
MwoI GCNNNNNNNGC 1 cut(s) 49
NdeI CATATG 1 cut(s) 206
NdeII GATC 2 cut(s) 244, 289
NlaIV GGNNCC 3 cut(s) 51, 88, 246
PaeR7I CTCGAG 1 cut(s) 137
PciSI GCTCTTC 1 cut(s) 168
PcsI WCGNNNNNNNCGW 1 cut(s) 186
PfeI GAWTC 2 cut(s) 115, 314
PkrI GCNGC 7 cut(s) 42, 45, 134, 222, 309, 329, 332
Psp124BI GAGCTC 1 cut(s) 180
PspN4I GGNNCC 3 cut(s) 51, 88, 246
PspPI GGNCC 2 cut(s) 269, 305
PspXI VCTCGAGB 1 cut(s) 137
PstNI CAGNNNCTG 1 cut(s) 333
PsuI RGATCY 1 cut(s) 244
PvuII CAGCTG 1 cut(s) 330
SacI GAGCTC 1 cut(s) 180
SapI GCTCTTC 1 cut(s) 168
SatI GCNGC 7 cut(s) 41, 44, 133, 221, 308, 328, 331
Sau3AI GATC 2 cut(s) 244, 289
Sau96I GGNCC 2 cut(s) 269, 305
SduI GDGCHC 1 cut(s) 180
SetI ASST 3 cut(s) 180, 274, 332
SfaNI GCATC 1 cut(s) 118
Sfr274I CTCGAG 1 cut(s) 137
SinI GGWCC 1 cut(s) 269
SlaI CTCGAG 1 cut(s) 137
SmlI CTYRAG 1 cut(s) 137
SmoI CTYRAG 1 cut(s) 137
Sse9I AATT 1 cut(s) 152
SsiI CCGC 3 cut(s) 46, 133, 308
SstI GAGCTC 1 cut(s) 180
TaqI TCGA 4 cut(s) 138, 180, 231, 292
TasI AATT 1 cut(s) 152
TauI GCSGC 2 cut(s) 135, 310
TfiI GAWTC 2 cut(s) 115, 314
TscAI CASTG 1 cut(s) 97
TseI GCWGC 5 cut(s) 40, 43, 220, 327, 330
TspRI CASTG 1 cut(s) 97
VpaK11BI GGWCC 1 cut(s) 269
XapI RAATTY 1 cut(s) 152
XhoI CTCGAG 1 cut(s) 137
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.