Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr03

Orthogroup ID

OG0005620

Physical Location & Seq

Genomic Coordinates Reverse (-)

34539426 .. 34543016

Gene Structure 3591 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD03G1258300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1053 bp

ATGTCAGAAGAGTACCCGGGATCGAGTTCGTCGCAACCATGTCCGGGTTTCGACCAGACGGTGATGATGATCCCGCCGCCGCAGCCGATGGAGGGCCTGAACGACACGGGCCCTCCGCCGTTTCTTAACAAGACGTACGACATGGTGGACGACCCGGGGTCGAACCGGGTCGTCTCGTGGAGCAAAGGAGGGGCTAGCTTTGTTGTTTGGGACCCACATGCTTTTGTGATGAGCCTCCTGCCCAAGTACTTCAAGCACAATAATTTCTCAAGCTTTGTGAGGCAGCTCAACACTTACGGTTTTAGAAAGGTTGATCCTAACAGATGGGAGTTTGCCAATGAAGGATTTGTAAGGGGTCAGAAGCATCTTTTGAAGAACATTAAAAGAAGGAAAGCACCTTCTCAGCCTTTTCCGGCACAGCAATCTGTTGGACCTTGCATGGAATTAGGCCACTTTGGGATAGATGGAGAAGTTGATCGTTTGCGGCATGACAAGCAGGTTCTAATGATGGAACTAGTGAAGCTTAGGCAGCAGCAGCAGAGTACTAGAGCCTACCTTCAGGCCATGGAGCAAAGGTTACAGGGGACAGAAATAAAGCAGCAACAGATGATGTCTTTCTTGGCAAGGGCAATGCAGAACCCGGCTTTTATACAGCAGCTAGTCCAACACAAGGAGAAAAGAAAGGAGCTTGAAGAGGCAATGACTAAGAAAAGGAGGAGGCCAATTGATCAAGGACCTGGTGATGTTGGTGGTGACGAATCAAACCAGGTTAAAAGGGAGCCTCTTGAATTTGGAGATTGTGAGTATGAAATGTCGGAGCTTGAAGCTCTTGCGATGGAAATGCAAGGATTAGGTAAGGTGGGAAAGGAACAGGAGGAAGCTGTAGAGCCGCTAGATAGCGGGGATAAAGAGCTTGATGAAGGATTTTGGGAAGAACTATTCAGTGAGAGGTTTGAGGGAGACTTGACCATTCCGAGTGCTGAAGTAGGCGAAGATGAAGATGTTATTATCTTGGCTGATCACTTAGGTTACTTTGGTTCATGGCCAAAGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003700 GO:0005488 GO:0005515 GO:0006355 GO:0006950 GO:0008150 GO:0009266 GO:0009314 GO:0009408 GO:0009416 GO:0009628 GO:0009642 GO:0009644 GO:0009889 GO:0010468 GO:0010556 GO:0019219 GO:0019222 GO:0031323 GO:0031326 GO:0032268 GO:0042802 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051246 GO:0051252 GO:0060255 GO:0065007 GO:0080090 GO:0140110 GO:1903506 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

ko00270 ko01100 map00270 map01100

Pfam Domains

Protein Families

HSF_DNA-bind

Protein Analysis

351

Amino Acids

39.92

Weight (kDa)

5.06

Isoelectric Point (pI)

57.61

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
HSF_DNA-bind	PF00447	41 - 130	1.7e-30	HSF-type DNA-binding

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0005620)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G51910
fragaria_vesca	FvH4_2g23000 FvH4_2g23000
malus_domestica	MD03G1258300.v1.1 MD11G1278900.v1.1
prunus_persica	Prupe.8G234900_v2.0.a1 Prupe.8G234900_v2.0.a1 Prupe.8G234900_v2.0.a1 Prupe.8G234900_v2.0.a1 Prupe.8G234900_v2.0.a1 Prupe.8G234900_v2.0.a1 Prupe.8G234900_v2.0.a1 Prupe.8G234900_v2.0.a1
pyrus_communis	pycom03g20460 pycom11g24650
rosa_chinensis	RchiOBHm_Chr6g0290041
rosa_laevigata	RLG00000012252
rosa_multiflora	Rmu_co8347871.1_g000001 Rmu_sc0002902.1_g000045 Rmu_sc0003927.1_g000002
rosa_roxburghii	Rroxscaffold_7G00177400
rosa_rugosa	Rorug06G0208300 Rorug06G0208400
rosa_samantha	Rh6AG319900 Rh6BG327000 Rh6CG332800 Rh6DG318700
rosa_wichuraiana	Rw6G027600

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	487
AciI	CCGC	7 cut(s)	74, 77, 80, 116, 484, 890, 900
AclWI	GGATC	3 cut(s)	28, 64, 308
AcoI	YGGCCR	1 cut(s)	1043
AcsI	RAATTY	1 cut(s)	788
AcuI	CTGAAG	2 cut(s)	542, 1002
AfaI	GTAC	4 cut(s)	14, 137, 248, 544
AfiI	CCNNNNNNNGG	3 cut(s)	44, 92, 670
AgsI	TTSAA	5 cut(s)	253, 373, 692, 788, 824
AhdI	GACNNNNNGTC	1 cut(s)	157
AhlI	ACTAGT	1 cut(s)	514
AjnI	CCWGG	2 cut(s)	736, 765
Alw26I	GTCTC	2 cut(s)	178, 954
AlwI	GGATC	3 cut(s)	28, 64, 308
Ama87I	CYCGRG	2 cut(s)	16, 154
AoxI	GGCC	6 cut(s)	94, 109, 448, 561, 719, 1043
ApaI	GGGCCC	1 cut(s)	113
ApeKI	GCWGC	7 cut(s)	82, 283, 529, 532, 535, 598, 655
ApoI	RAATTY	1 cut(s)	788
AspS9I	GGNCC	6 cut(s)	94, 109, 110, 211, 431, 734
AsuC2I	CCSGG	7 cut(s)	17, 18, 45, 155, 156, 167, 641
AsuHPI	GGTGA	3 cut(s)	73, 752, 764
AsuNHI	GCTAGC	1 cut(s)	194
AvaI	CYCGRG	2 cut(s)	16, 154
AvaII	GGWCC	3 cut(s)	211, 431, 734
BaeGI	GKGCMC	1 cut(s)	113
BalI	TGGCCA	1 cut(s)	1045
BanII	GRGCYC	1 cut(s)	113
BauI	CACGAG	1 cut(s)	175
BbvI	GCAGC	7 cut(s)	94, 295, 541, 544, 547, 610, 667
BccI	CCATC	5 cut(s)	82, 318, 458, 502, 829
BceAI	ACGGC	1 cut(s)	103
BcgI	CGANNNNNNTGC	2 cut(s)	823, 857
BciT130I	CCWGG	2 cut(s)	738, 767
BclI	TGATCA	2 cut(s)	727, 1018
BcnI	CCSGG	7 cut(s)	17, 18, 45, 155, 156, 167, 641
BcoDI	GTCTC	2 cut(s)	178, 954
BcuI	ACTAGT	1 cut(s)	514
BfaI	CTAG	5 cut(s)	195, 515, 546, 659, 893
BfmI	CTRYAG	1 cut(s)	882
BfuAI	ACCTGC	1 cut(s)	487
BmcAI	AGTACT	2 cut(s)	248, 544
Bme1390I	CCNGG	9 cut(s)	17, 18, 45, 155, 156, 167, 641, 738, 767
Bme18I	GGWCC	3 cut(s)	211, 431, 734
BmeRI	GACNNNNNGTC	1 cut(s)	157
BmeT110I	CYCGRG	2 cut(s)	16, 154
BmgT120I	GGNCC	6 cut(s)	94, 109, 110, 211, 431, 734
BmiI	GGNNCC	4 cut(s)	111, 212, 213, 780
BmrFI	CCNGG	9 cut(s)	17, 18, 45, 155, 156, 167, 641, 738, 767
BmsI	GCATC	1 cut(s)	373
BmtI	GCTAGC	1 cut(s)	198
Bpu10I	CCTNAGC	1 cut(s)	524
BpuEI	CTTGAG	1 cut(s)	253
BpuMI	CCSGG	7 cut(s)	17, 18, 45, 155, 156, 167, 641
BsaBI	GATNNNNATC	1 cut(s)	68
BsaJI	CCNNGG	4 cut(s)	16, 154, 155, 564
BsaXI	ACNNNNNCTCC	4 cut(s)	97, 127, 560, 590
Bsc4I	CCNNNNNNNGG	3 cut(s)	44, 92, 670
Bse3DI	GCAATG	2 cut(s)	636, 705
Bse8I	GATNNNNATC	1 cut(s)	68
BseBI	CCWGG	2 cut(s)	738, 767
BseDI	CCNNGG	4 cut(s)	16, 154, 155, 564
BseJI	GATNNNNATC	1 cut(s)	68
BseLI	CCNNNNNNNGG	3 cut(s)	44, 92, 670
BseMI	GCAATG	2 cut(s)	636, 705
BseMII	CTCAG	1 cut(s)	416
BseRI	GAGGAG	1 cut(s)	730
BseSI	GKGCMC	1 cut(s)	113
BseXI	GCAGC	7 cut(s)	94, 295, 541, 544, 547, 610, 667
BshFI	GGCC	6 cut(s)	96, 111, 450, 563, 721, 1045
BsiHKCI	CYCGRG	2 cut(s)	16, 154
BsiSI	CCGG	6 cut(s)	17, 44, 155, 166, 413, 641
BsiWI	CGTACG	1 cut(s)	135
BslFI	GGGAC	2 cut(s)	224, 598
BslI	CCNNNNNNNGG	3 cut(s)	44, 92, 670
BsmAI	GTCTC	2 cut(s)	178, 954
BsmBI	CGTCTC	1 cut(s)	178
BsmFI	GGGAC	2 cut(s)	224, 598
BsnI	GGCC	6 cut(s)	96, 111, 450, 563, 721, 1045
BsoBI	CYCGRG	2 cut(s)	16, 154
Bsp120I	GGGCCC	1 cut(s)	109
Bsp1286I	GDGCHC	1 cut(s)	113
Bsp143I	GATC	6 cut(s)	20, 69, 313, 475, 727, 1018
Bsp19I	CCATGG	1 cut(s)	564
BspACI	CCGC	7 cut(s)	74, 77, 80, 116, 484, 890, 900
BspANI	GGCC	6 cut(s)	96, 111, 450, 563, 721, 1045
BspCNI	CTCAG	1 cut(s)	415
BspLI	GGNNCC	4 cut(s)	111, 212, 213, 780
BspMI	ACCTGC	1 cut(s)	487
BspOI	GCTAGC	1 cut(s)	198
BspPI	GGATC	3 cut(s)	28, 64, 308
BsrDI	GCAATG	2 cut(s)	636, 705
BssECI	CCNNGG	4 cut(s)	16, 154, 155, 564
BssMI	GATC	6 cut(s)	20, 69, 313, 475, 727, 1018
BssSI	CACGAG	1 cut(s)	175
BssT1I	CCWWGG	1 cut(s)	564
Bst2BI	CACGAG	1 cut(s)	175
Bst2UI	CCWGG	2 cut(s)	738, 767
Bst4CI	ACNGT	2 cut(s)	61, 299
Bst6I	CTCTTC	2 cut(s)	3, 687
BstC8I	GCNNGC	1 cut(s)	196
BstDEI	CTNAG	4 cut(s)	402, 524, 705, 1024
BstDSI	CCRYGG	1 cut(s)	564
BstKTI	GATC	6 cut(s)	23, 72, 316, 478, 730, 1021
BstMAI	GTCTC	2 cut(s)	178, 954
BstMBI	GATC	6 cut(s)	20, 69, 313, 475, 727, 1018
BstMWI	GCNNNNNNNGC	4 cut(s)	82, 493, 529, 535
BstNI	CCWGG	2 cut(s)	738, 767
BstNSI	RCATGY	1 cut(s)	221
BstSCI	CCNGG	9 cut(s)	15, 16, 43, 153, 154, 165, 639, 736, 765
BstSFI	CTRYAG	1 cut(s)	882
BstSLI	GKGCMC	1 cut(s)	113
BstV1I	GCAGC	7 cut(s)	94, 295, 541, 544, 547, 610, 667
BsuRI	GGCC	6 cut(s)	96, 111, 450, 563, 721, 1045
BtgI	CCRYGG	1 cut(s)	564
BtgZI	GCGATG	1 cut(s)	848
BtsIMutI	CAGTG	1 cut(s)	949
BveI	ACCTGC	1 cut(s)	487
Cac8I	GCNNGC	1 cut(s)	196
Cfr13I	GGNCC	6 cut(s)	94, 109, 110, 211, 431, 734
Cfr9I	CCCGGG	2 cut(s)	16, 154
CsiI	ACCWGGT	2 cut(s)	736, 765
Csp6I	GTAC	4 cut(s)	13, 136, 247, 543
CviAII	CATG	7 cut(s)	39, 142, 218, 439, 488, 565, 1041
CviQI	GTAC	4 cut(s)	13, 136, 247, 543
DdeI	CTNAG	4 cut(s)	402, 524, 705, 1024
DpnI	GATC	6 cut(s)	22, 71, 315, 477, 729, 1020
DpnII	GATC	6 cut(s)	20, 69, 313, 475, 727, 1018
DriI	GACNNNNNGTC	1 cut(s)	157
EaeI	YGGCCR	1 cut(s)	1043
Eam1104I	CTCTTC	2 cut(s)	3, 687
Eam1105I	GACNNNNNGTC	1 cut(s)	157
EarI	CTCTTC	2 cut(s)	3, 687
EciI	GGCGGA	1 cut(s)	105
Eco130I	CCWWGG	1 cut(s)	564
Eco24I	GRGCYC	1 cut(s)	113
Eco47I	GGWCC	3 cut(s)	211, 431, 734
Eco57I	CTGAAG	2 cut(s)	542, 1002
Eco88I	CYCGRG	2 cut(s)	16, 154
EcoO109I	RGGNCCY	4 cut(s)	94, 110, 211, 734
EcoRII	CCWGG	2 cut(s)	736, 765
EcoT14I	CCWWGG	1 cut(s)	564
EcoT38I	GRGCYC	1 cut(s)	113
ErhI	CCWWGG	1 cut(s)	564
Esp3I	CGTCTC	1 cut(s)	178
FaeI	CATG	7 cut(s)	42, 145, 221, 442, 491, 568, 1044
FaiI	YATR	9 cut(s)	40, 143, 219, 440, 489, 566, 650, 807, 1042
FaqI	GGGAC	2 cut(s)	224, 598
FatI	CATG	7 cut(s)	38, 141, 217, 438, 487, 564, 1040
FauI	CCCGC	2 cut(s)	81, 893
FbaI	TGATCA	2 cut(s)	727, 1018
FriOI	GRGCYC	1 cut(s)	113
FspBI	CTAG	5 cut(s)	195, 515, 546, 659, 893
HaeIII	GGCC	6 cut(s)	96, 111, 450, 563, 721, 1045
HapII	CCGG	6 cut(s)	17, 44, 155, 166, 413, 641
Hin1II	CATG	7 cut(s)	42, 145, 221, 442, 491, 568, 1044
HindIII	AAGCTT	2 cut(s)	271, 521
HinfI	GANTC	1 cut(s)	758
HpaII	CCGG	6 cut(s)	17, 44, 155, 166, 413, 641
HphI	GGTGA	3 cut(s)	73, 752, 764
Hpy166II	GTNNAC	1 cut(s)	148
Hpy188I	TCNGA	4 cut(s)	7, 360, 817, 975
Hpy188III	TCNNGA	1 cut(s)	785
Hpy8I	GTNNAC	1 cut(s)	148
Hpy99I	CGWCG	1 cut(s)	34
HpyAV	CCTTC	5 cut(s)	335, 381, 408, 566, 914
HpyCH4III	ACNGT	2 cut(s)	61, 299
HpyCH4IV	ACGT	1 cut(s)	134
HpyCH4V	TGCA	3 cut(s)	438, 634, 844
HpyF10VI	GCNNNNNNNGC	4 cut(s)	82, 493, 529, 535
HpyF3I	CTNAG	4 cut(s)	402, 524, 705, 1024
HpySE526I	ACGT	1 cut(s)	134
Hsp92II	CATG	7 cut(s)	42, 145, 221, 442, 491, 568, 1044
KflI	GGGWCCC	1 cut(s)	211
Ksp22I	TGATCA	2 cut(s)	727, 1018
Kzo9I	GATC	6 cut(s)	20, 69, 313, 475, 727, 1018
LmnI	GCTCC	5 cut(s)	180, 568, 685, 778, 817
Lsp1109I	GCAGC	7 cut(s)	94, 295, 541, 544, 547, 610, 667
LweI	GCATC	1 cut(s)	373
MabI	ACCWGGT	2 cut(s)	736, 765
MaeI	CTAG	5 cut(s)	195, 515, 546, 659, 893
MaeII	ACGT	1 cut(s)	134
MaeIII	GTNAC	3 cut(s)	576, 752, 1028
MalI	GATC	6 cut(s)	22, 71, 315, 477, 729, 1020
MboI	GATC	6 cut(s)	20, 69, 313, 475, 727, 1018
MboII	GAAGA	6 cut(s)	20, 385, 704, 944, 1004, 1010
MfeI	CAATTG	1 cut(s)	723
MhlI	GDGCHC	1 cut(s)	113
MlsI	TGGCCA	1 cut(s)	1045
MluCI	AATT	4 cut(s)	262, 443, 723, 788
MluNI	TGGCCA	1 cut(s)	1045
MmeI	TCCRAC	3 cut(s)	409, 688, 795
Mox20I	TGGCCA	1 cut(s)	1045
MscI	TGGCCA	1 cut(s)	1045
MseI	TTAA	3 cut(s)	126, 381, 771
Msp20I	TGGCCA	1 cut(s)	1045
MspI	CCGG	6 cut(s)	17, 44, 155, 166, 413, 641
MspR9I	CCNGG	9 cut(s)	17, 18, 45, 155, 156, 167, 641, 738, 767
MunI	CAATTG	1 cut(s)	723
MvaI	CCWGG	2 cut(s)	738, 767
MwoI	GCNNNNNNNGC	4 cut(s)	82, 493, 529, 535
NciI	CCSGG	7 cut(s)	17, 18, 45, 155, 156, 167, 641
NcoI	CCATGG	1 cut(s)	564
NdeII	GATC	6 cut(s)	20, 69, 313, 475, 727, 1018
NheI	GCTAGC	1 cut(s)	194
NlaIII	CATG	7 cut(s)	42, 145, 221, 442, 491, 568, 1044
NlaIV	GGNNCC	4 cut(s)	111, 212, 213, 780
NmuCI	GTSAC	1 cut(s)	752
NspI	RCATGY	1 cut(s)	221
PfeI	GAWTC	1 cut(s)	758
Pfl23II	CGTACG	1 cut(s)	135
PpuMI	RGGWCCY	2 cut(s)	211, 734
Psp5II	RGGWCCY	2 cut(s)	211, 734
Psp6I	CCWGG	2 cut(s)	736, 765
PspGI	CCWGG	2 cut(s)	736, 765
PspLI	CGTACG	1 cut(s)	135
PspN4I	GGNNCC	4 cut(s)	111, 212, 213, 780
PspOMI	GGGCCC	1 cut(s)	109
PspPI	GGNCC	6 cut(s)	94, 109, 110, 211, 431, 734
PspPPI	RGGWCCY	2 cut(s)	211, 734
RsaI	GTAC	4 cut(s)	14, 137, 248, 544
RsaNI	GTAC	4 cut(s)	13, 136, 247, 543
SaqAI	TTAA	3 cut(s)	126, 381, 771
Sau3AI	GATC	6 cut(s)	20, 69, 313, 475, 727, 1018
Sau96I	GGNCC	6 cut(s)	94, 109, 110, 211, 431, 734
ScaI	AGTACT	2 cut(s)	248, 544
ScrFI	CCNGG	9 cut(s)	17, 18, 45, 155, 156, 167, 641, 738, 767
SduI	GDGCHC	1 cut(s)	113
SexAI	ACCWGGT	2 cut(s)	736, 765
SfaNI	GCATC	1 cut(s)	373
SfcI	CTRYAG	1 cut(s)	882
SinI	GGWCC	3 cut(s)	211, 431, 734
SmaI	CCCGGG	2 cut(s)	18, 156
SmlI	CTYRAG	1 cut(s)	268
SmoI	CTYRAG	1 cut(s)	268
SpeI	ACTAGT	1 cut(s)	514
Sse9I	AATT	4 cut(s)	262, 443, 723, 788
SsiI	CCGC	7 cut(s)	74, 77, 80, 116, 484, 890, 900
SspMI	CTAG	5 cut(s)	195, 515, 546, 659, 893
StyD4I	CCNGG	9 cut(s)	15, 16, 43, 153, 154, 165, 639, 736, 765
StyI	CCWWGG	1 cut(s)	564
TaaI	ACNGT	2 cut(s)	61, 299
TaiI	ACGT	1 cut(s)	137
TaqI	TCGA	3 cut(s)	23, 51, 161
TasI	AATT	4 cut(s)	262, 443, 723, 788
TatI	WGTACW	2 cut(s)	246, 542
TauI	GCSGC	4 cut(s)	79, 82, 487, 892
TfiI	GAWTC	1 cut(s)	758
Tru1I	TTAA	3 cut(s)	126, 381, 771
Tru9I	TTAA	3 cut(s)	126, 381, 771
TscAI	CASTG	1 cut(s)	949
TseFI	GTSAC	1 cut(s)	752
TseI	GCWGC	7 cut(s)	82, 283, 529, 532, 535, 598, 655
Tsp45I	GTSAC	1 cut(s)	752
TspDTI	ATGAA	5 cut(s)	354, 822, 933, 1011, 1029
TspMI	CCCGGG	2 cut(s)	16, 154
TspRI	CASTG	1 cut(s)	949
VpaK11BI	GGWCC	3 cut(s)	211, 431, 734
XapI	RAATTY	1 cut(s)	788
XceI	RCATGY	1 cut(s)	221
XmaI	CCCGGG	2 cut(s)	16, 154
XspI	CTAG	5 cut(s)	195, 515, 546, 659, 893
ZrmI	AGTACT	2 cut(s)	248, 544

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.