MD11G1278900.v1.1

Heat Stress Transcription Factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0005620
Physical Location & Seq
Reverse (-)
39604890 .. 39608513
3624 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1278900.v1.1.491

Sequence Viewer

Length: 1128 bp
ATGACAACCGCAGCTCTGCCTTTTATCTTGCATTTCAACAGCAACCCATTGAAAGAAGAGTACCCGGGTTCGAGTTCGAGTTCGTCTCAATCGGGTCCAGATTTCGACCCGATGGTGATGATGATCCCTCCGCCGCAGCCGATGGAGGGCCTGAACGACACGGGCCCTCCGCCGTTTCTGAACAAGACATACGATATGGTGGACGACCCGGATTCGAATCGGGTGGTGTCGTGGAGCAGGGAAGGGGGGAGCTTTGTGGTGTGGGACCCACATGCTTTTGTGATGAGCCTCCTGCCTAGGTACTTCAAGCACAATAATTTCTCAAGCTTTGTGAGGCAGCTCAACACTTACGGTTTTAGAAAGGTTGATCCTGACCGATGGGAGTTTGCCAATGAAGGATTTGTAAGGGGTCAGAAGCATCTTTTGAAGAACATTAGAAGAAGAAAGGCACCTTCTCAGCCTTTTCCGGCACAGCAAACTGTCAGCACTTGTGTGGAACTTGGCCAGTTTGGGCTGGATGGAGAAGTTGATCGGTTGCGGCGTGACAAGCAGGTTCTAATTATGGAACTAGTGAAGCTTGGGCAGCAGCAGCAGAGTACTAGAGCCTACCTTCAGGCCATGGAACAAAGGTTACAAAAGACAGAAATGAAGCAGCGGCAGATGATGGCTTTCTTGGCAAGGGCAATGCAGAACCCGGCTTTTATACAGCAGCTTGTCCAACACAAGGAGAAAAGGAAGGAGCTTGAAGAGGCAATGACTAAGAAAAGGAGGAGGCCAATTGATCAAGGACCTAGTGGTGTTGGTGGTGGCGAATCAGGCCTAAGAGGCAAAGGAATAAACCCAGTTAAAAATGAGCCTCTTGAATTTGGCGATTGTGAGTATGAAATGTCGGAGCTAGAAGCACTTGCGATGGAAATGCAAGGATTAGGTAAGGTGAGAAAGGAACAGGAGGAAGTGGTAGAGCCACTAGATAGCGGAGATAAGGAGCTTGATGAAGGATTTTGGGAAGAACTATTTAGTGAGAGATTCAAGGGAGACTTGACCGTTCCGAGTGCTGAAGTAGGCGAAGATGAAGATGTGATTATCTTGGCTGATCGCTTAGGTTACGTAGGTTCATCCCCAAAGTAG

Protein Analysis

376

Amino Acids

42.6

Weight (kDa)

5.35

Isoelectric Point (pI)

54.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HSF_DNA-bind PF00447 59 - 148 2.2e-30 HSF-type DNA-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 541
AccB1I GGYRCC 1 cut(s) 448
AciI CCGC 7 cut(s) 9, 131, 134, 170, 538, 655, 975
AclWI GGATC 2 cut(s) 118, 362
AcoI YGGCCR 1 cut(s) 502
AcsI RAATTY 1 cut(s) 863
AcuI CTGAAG 2 cut(s) 596, 1077
AfaI GTAC 3 cut(s) 62, 302, 598
AfiI CCNNNNNNNGG 2 cut(s) 146, 724
AgsI TTSAA 7 cut(s) 37, 52, 307, 427, 746, 863, 1030
AhlI ACTAGT 1 cut(s) 568
AleI CACNNNNGTG 1 cut(s) 491
AluBI AGCT 9 cut(s) 14, 252, 327, 340, 577, 712, 742, 895, 988
AluI AGCT 9 cut(s) 14, 252, 327, 340, 577, 712, 742, 895, 988
Alw26I GTCTC 2 cut(s) 90, 1029
AlwI GGATC 2 cut(s) 118, 362
Ama87I CYCGRG 1 cut(s) 64
AoxI GGCC 6 cut(s) 148, 163, 502, 615, 773, 817
ApaI GGGCCC 1 cut(s) 167
ApeKI GCWGC 8 cut(s) 11, 136, 337, 583, 586, 589, 652, 709
ApoI RAATTY 1 cut(s) 863
AspA2I CCTAGG 1 cut(s) 296
AspS9I GGNCC 6 cut(s) 95, 148, 163, 164, 265, 788
AsuC2I CCSGG 4 cut(s) 65, 66, 209, 695
AsuHPI GGTGA 2 cut(s) 127, 946
AsuII TTCGAA 1 cut(s) 215
AvaI CYCGRG 1 cut(s) 64
AvaII GGWCC 3 cut(s) 95, 265, 788
AvrII CCTAGG 1 cut(s) 296
BaeGI GKGCMC 1 cut(s) 167
BalI TGGCCA 1 cut(s) 504
BanI GGYRCC 1 cut(s) 448
BanII GRGCYC 1 cut(s) 167
BbvI GCAGC 8 cut(s) 23, 148, 349, 595, 598, 601, 664, 721
BccI CCATC 6 cut(s) 106, 136, 372, 512, 658, 904
BceAI ACGGC 1 cut(s) 157
BcgI CGANNNNNNTGC 2 cut(s) 898, 932
BclI TGATCA 1 cut(s) 781
BcnI CCSGG 4 cut(s) 65, 66, 209, 695
BcoDI GTCTC 2 cut(s) 90, 1029
BcuI ACTAGT 1 cut(s) 568
BfaI CTAG 6 cut(s) 297, 569, 600, 792, 896, 968
BfuAI ACCTGC 1 cut(s) 541
BglI GCCNNNNNGGC 1 cut(s) 825
BlnI CCTAGG 1 cut(s) 296
BmcAI AGTACT 1 cut(s) 598
Bme1390I CCNGG 4 cut(s) 65, 66, 209, 695
Bme18I GGWCC 3 cut(s) 95, 265, 788
BmeT110I CYCGRG 1 cut(s) 64
BmgT120I GGNCC 6 cut(s) 95, 148, 163, 164, 265, 788
BmiI GGNNCC 5 cut(s) 96, 165, 266, 267, 450
BmrFI CCNGG 4 cut(s) 65, 66, 209, 695
BmrI ACTGGG 1 cut(s) 836
BmsI GCATC 1 cut(s) 427
BmuI ACTGGG 1 cut(s) 836
BplI GAGNNNNNCTC 2 cut(s) 70, 102
Bpu10I CCTNAGC 1 cut(s) 1099
Bpu14I TTCGAA 1 cut(s) 215
BpuEI CTTGAG 1 cut(s) 307
BpuMI CCSGG 4 cut(s) 65, 66, 209, 695
BsaAI YACGTR 1 cut(s) 1108
BsaBI GATNNNNATC 2 cut(s) 122, 216
BsaJI CCNNGG 3 cut(s) 64, 296, 618
BsaXI ACNNNNNCTCC 2 cut(s) 151, 181
Bsc4I CCNNNNNNNGG 2 cut(s) 146, 724
Bse1I ACTGG 2 cut(s) 505, 842
Bse3DI GCAATG 2 cut(s) 690, 759
Bse8I GATNNNNATC 2 cut(s) 122, 216
BseDI CCNNGG 3 cut(s) 64, 296, 618
BseGI GGATG 2 cut(s) 523, 1115
BseJI GATNNNNATC 2 cut(s) 122, 216
BseLI CCNNNNNNNGG 2 cut(s) 146, 724
BseMI GCAATG 2 cut(s) 690, 759
BseMII CTCAG 1 cut(s) 470
BseNI ACTGG 2 cut(s) 505, 842
BseRI GAGGAG 1 cut(s) 784
BseSI GKGCMC 1 cut(s) 167
BseXI GCAGC 8 cut(s) 23, 148, 349, 595, 598, 601, 664, 721
BshFI GGCC 6 cut(s) 150, 165, 504, 617, 775, 819
BshNI GGYRCC 1 cut(s) 448
BsiHKCI CYCGRG 1 cut(s) 64
BsiSI CCGG 4 cut(s) 65, 209, 467, 695
BslFI GGGAC 1 cut(s) 278
BslI CCNNNNNNNGG 2 cut(s) 146, 724
BsmAI GTCTC 2 cut(s) 90, 1029
BsmBI CGTCTC 1 cut(s) 90
BsmFI GGGAC 1 cut(s) 278
BsnI GGCC 6 cut(s) 150, 165, 504, 617, 775, 819
BsoBI CYCGRG 1 cut(s) 64
Bsp119I TTCGAA 1 cut(s) 215
Bsp120I GGGCCC 1 cut(s) 163
Bsp1286I GDGCHC 1 cut(s) 167
Bsp143I GATC 5 cut(s) 123, 367, 529, 781, 1093
Bsp19I CCATGG 1 cut(s) 618
BspACI CCGC 7 cut(s) 9, 131, 134, 170, 538, 655, 975
BspANI GGCC 6 cut(s) 150, 165, 504, 617, 775, 819
BspCNI CTCAG 1 cut(s) 469
BspLI GGNNCC 5 cut(s) 96, 165, 266, 267, 450
BspMI ACCTGC 1 cut(s) 541
BspPI GGATC 2 cut(s) 118, 362
BspT104I TTCGAA 1 cut(s) 215
BspT107I GGYRCC 1 cut(s) 448
BsrDI GCAATG 2 cut(s) 690, 759
BsrI ACTGG 2 cut(s) 505, 842
BssECI CCNNGG 3 cut(s) 64, 296, 618
BssMI GATC 5 cut(s) 123, 367, 529, 781, 1093
BssT1I CCWWGG 2 cut(s) 296, 618
Bst4CI ACNGT 3 cut(s) 353, 481, 1045
Bst6I CTCTTC 2 cut(s) 51, 741
BstBAI YACGTR 1 cut(s) 1108
BstBI TTCGAA 1 cut(s) 215
BstDEI CTNAG 4 cut(s) 456, 759, 821, 1099
BstDSI CCRYGG 1 cut(s) 618
BstF5I GGATG 2 cut(s) 523, 1115
BstKTI GATC 5 cut(s) 126, 370, 532, 784, 1096
BstMAI GTCTC 2 cut(s) 90, 1029
BstMBI GATC 5 cut(s) 123, 367, 529, 781, 1093
BstMWI GCNNNNNNNGC 6 cut(s) 547, 583, 589, 674, 816, 825
BstNSI RCATGY 1 cut(s) 275
BstSCI CCNGG 4 cut(s) 63, 64, 207, 693
BstSLI GKGCMC 1 cut(s) 167
BstSNI TACGTA 1 cut(s) 1108
BstV1I GCAGC 8 cut(s) 23, 148, 349, 595, 598, 601, 664, 721
BsuRI GGCC 6 cut(s) 150, 165, 504, 617, 775, 819
BtgI CCRYGG 1 cut(s) 618
BtgZI GCGATG 1 cut(s) 923
BtsCI GGATG 2 cut(s) 523, 1115
BveI ACCTGC 1 cut(s) 541
Cfr13I GGNCC 6 cut(s) 95, 148, 163, 164, 265, 788
Cfr9I CCCGGG 1 cut(s) 64
Csp6I GTAC 3 cut(s) 61, 301, 597
CviAII CATG 2 cut(s) 272, 619
CviQI GTAC 3 cut(s) 61, 301, 597
DdeI CTNAG 4 cut(s) 456, 759, 821, 1099
DpnI GATC 5 cut(s) 125, 369, 531, 783, 1095
DpnII GATC 5 cut(s) 123, 367, 529, 781, 1093
EaeI YGGCCR 1 cut(s) 502
Eam1104I CTCTTC 2 cut(s) 51, 741
EarI CTCTTC 2 cut(s) 51, 741
EciI GGCGGA 2 cut(s) 120, 159
Eco105I TACGTA 1 cut(s) 1108
Eco130I CCWWGG 2 cut(s) 296, 618
Eco147I AGGCCT 1 cut(s) 819
Eco24I GRGCYC 1 cut(s) 167
Eco47I GGWCC 3 cut(s) 95, 265, 788
Eco57I CTGAAG 2 cut(s) 596, 1077
Eco88I CYCGRG 1 cut(s) 64
EcoO109I RGGNCCY 4 cut(s) 148, 164, 265, 788
EcoT14I CCWWGG 2 cut(s) 296, 618
EcoT38I GRGCYC 1 cut(s) 167
ErhI CCWWGG 2 cut(s) 296, 618
Esp3I CGTCTC 1 cut(s) 90
FaeI CATG 2 cut(s) 275, 622
FaiI YATR 7 cut(s) 190, 197, 273, 563, 620, 704, 882
FalI AAGNNNNNCTT 2 cut(s) 1022, 1054
FaqI GGGAC 1 cut(s) 278
FatI CATG 2 cut(s) 271, 618
FbaI TGATCA 1 cut(s) 781
FokI GGATG 2 cut(s) 530, 1102
FriOI GRGCYC 1 cut(s) 167
FspBI CTAG 6 cut(s) 297, 569, 600, 792, 896, 968
HaeIII GGCC 6 cut(s) 150, 165, 504, 617, 775, 819
HapII CCGG 4 cut(s) 65, 209, 467, 695
Hin1II CATG 2 cut(s) 275, 622
HindIII AAGCTT 2 cut(s) 325, 575
HinfI GANTC 4 cut(s) 212, 217, 812, 1026
HpaII CCGG 4 cut(s) 65, 209, 467, 695
HphI GGTGA 2 cut(s) 127, 946
Hpy166II GTNNAC 1 cut(s) 202
Hpy188I TCNGA 4 cut(s) 180, 414, 892, 1050
Hpy188III TCNNGA 3 cut(s) 98, 371, 860
Hpy8I GTNNAC 1 cut(s) 202
HpyAV CCTTC 6 cut(s) 236, 389, 462, 620, 730, 989
HpyCH4III ACNGT 3 cut(s) 353, 481, 1045
HpyCH4IV ACGT 1 cut(s) 1107
HpyCH4V TGCA 3 cut(s) 31, 688, 919
HpyF10VI GCNNNNNNNGC 6 cut(s) 547, 583, 589, 674, 816, 825
HpyF3I CTNAG 4 cut(s) 456, 759, 821, 1099
HpySE526I ACGT 1 cut(s) 1107
Hsp92II CATG 2 cut(s) 275, 622
KflI GGGWCCC 1 cut(s) 265
Ksp22I TGATCA 1 cut(s) 781
Kzo9I GATC 5 cut(s) 123, 367, 529, 781, 1093
LmnI GCTCC 5 cut(s) 234, 249, 739, 892, 985
Lsp1109I GCAGC 8 cut(s) 23, 148, 349, 595, 598, 601, 664, 721
LweI GCATC 1 cut(s) 427
MaeI CTAG 6 cut(s) 297, 569, 600, 792, 896, 968
MaeII ACGT 1 cut(s) 1107
MaeIII GTNAC 3 cut(s) 542, 630, 1103
MalI GATC 5 cut(s) 125, 369, 531, 783, 1095
MboI GATC 5 cut(s) 123, 367, 529, 781, 1093
MboII GAAGA 8 cut(s) 68, 439, 450, 453, 758, 1019, 1079, 1085
MfeI CAATTG 1 cut(s) 777
MhlI GDGCHC 1 cut(s) 167
MlsI TGGCCA 1 cut(s) 504
MluCI AATT 4 cut(s) 316, 558, 777, 863
MluNI TGGCCA 1 cut(s) 504
MmeI TCCRAC 2 cut(s) 742, 870
Mox20I TGGCCA 1 cut(s) 504
MscI TGGCCA 1 cut(s) 504
MseI TTAA 1 cut(s) 846
MslI CAYNNNNRTG 1 cut(s) 491
Msp20I TGGCCA 1 cut(s) 504
MspA1I CMGCKG 1 cut(s) 655
MspI CCGG 4 cut(s) 65, 209, 467, 695
MspR9I CCNGG 4 cut(s) 65, 66, 209, 695
MunI CAATTG 1 cut(s) 777
MwoI GCNNNNNNNGC 6 cut(s) 547, 583, 589, 674, 816, 825
NciI CCSGG 4 cut(s) 65, 66, 209, 695
NcoI CCATGG 1 cut(s) 618
NdeII GATC 5 cut(s) 123, 367, 529, 781, 1093
NlaIII CATG 2 cut(s) 275, 622
NlaIV GGNNCC 5 cut(s) 96, 165, 266, 267, 450
NmuCI GTSAC 1 cut(s) 542
NspI RCATGY 1 cut(s) 275
NspV TTCGAA 1 cut(s) 215
OliI CACNNNNGTG 1 cut(s) 491
PceI AGGCCT 1 cut(s) 819
PcsI WCGNNNNNNNCGW 1 cut(s) 538
PfeI GAWTC 4 cut(s) 212, 217, 812, 1026
Ppu21I YACGTR 1 cut(s) 1108
PpuMI RGGWCCY 2 cut(s) 265, 788
Psp5II RGGWCCY 2 cut(s) 265, 788
PspN4I GGNNCC 5 cut(s) 96, 165, 266, 267, 450
PspOMI GGGCCC 1 cut(s) 163
PspPI GGNCC 6 cut(s) 95, 148, 163, 164, 265, 788
PspPPI RGGWCCY 2 cut(s) 265, 788
PsrI GAACNNNNNNTAC 4 cut(s) 173, 205, 615, 647
RsaI GTAC 3 cut(s) 62, 302, 598
RsaNI GTAC 3 cut(s) 61, 301, 597
RseI CAYNNNNRTG 1 cut(s) 491
SaqAI TTAA 1 cut(s) 846
Sau3AI GATC 5 cut(s) 123, 367, 529, 781, 1093
Sau96I GGNCC 6 cut(s) 95, 148, 163, 164, 265, 788
ScaI AGTACT 1 cut(s) 598
ScrFI CCNGG 4 cut(s) 65, 66, 209, 695
SduI GDGCHC 1 cut(s) 167
SfaNI GCATC 1 cut(s) 427
SfuI TTCGAA 1 cut(s) 215
SinI GGWCC 3 cut(s) 95, 265, 788
SmaI CCCGGG 1 cut(s) 66
SmiMI CAYNNNNRTG 1 cut(s) 491
SmlI CTYRAG 1 cut(s) 322
SmoI CTYRAG 1 cut(s) 322
SnaBI TACGTA 1 cut(s) 1108
SpeI ACTAGT 1 cut(s) 568
Sse9I AATT 4 cut(s) 316, 558, 777, 863
SseBI AGGCCT 1 cut(s) 819
SsiI CCGC 7 cut(s) 9, 131, 134, 170, 538, 655, 975
SspMI CTAG 6 cut(s) 297, 569, 600, 792, 896, 968
StuI AGGCCT 1 cut(s) 819
StyD4I CCNGG 4 cut(s) 63, 64, 207, 693
StyI CCWWGG 2 cut(s) 296, 618
TaaI ACNGT 3 cut(s) 353, 481, 1045
TaiI ACGT 1 cut(s) 1110
TaqI TCGA 4 cut(s) 71, 77, 105, 215
TaqII GACCGA 1 cut(s) 390
TasI AATT 4 cut(s) 316, 558, 777, 863
TatI WGTACW 1 cut(s) 596
TauI GCSGC 3 cut(s) 136, 541, 658
TfiI GAWTC 4 cut(s) 212, 217, 812, 1026
Tru1I TTAA 1 cut(s) 846
Tru9I TTAA 1 cut(s) 846
TseFI GTSAC 1 cut(s) 542
TseI GCWGC 8 cut(s) 11, 136, 337, 583, 586, 589, 652, 709
Tsp45I GTSAC 1 cut(s) 542
TspDTI ATGAA 6 cut(s) 408, 662, 897, 1008, 1086, 1104
TspMI CCCGGG 1 cut(s) 64
VpaK11BI GGWCC 3 cut(s) 95, 265, 788
XapI RAATTY 1 cut(s) 863
XceI RCATGY 1 cut(s) 275
XmaI CCCGGG 1 cut(s) 64
XmaJI CCTAGG 1 cut(s) 296
XspI CTAG 6 cut(s) 297, 569, 600, 792, 896, 968
ZrmI AGTACT 1 cut(s) 598
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.