Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr03

Orthogroup ID

OG0009825

Physical Location & Seq

Genomic Coordinates Reverse (-)

34626426 .. 34631071

Gene Structure 4646 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD03G1259300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1500 bp

ATGGAAGAATCACTACCAATGTTAACCACTCGCCGGACTGACCAGAGTCCGGTCAACCACCGTGACCACCTCCCCAAAAGCCCAAGAGTTCCAATGGAGTTTCTCTCAAGGTCATGGAGTGCCTCTGCTCTTGAAGTCTCCAAAGCTCTGTCTCAGTATCACCCTGCTCCTCTGCCTCCTTCTTCATGCATGGCTAATTCCAAATCCTCAAATAGCTCTTCCTCTTGCACCACCGCCACTCCTTCCAATCCTGAAAACATTGCCGGAGAAGCAGAGGAGCCGCTGACCCACCCCGGAAACCAATTTTACTTTGCTTCCTCTGTCACTTCCCAGCTCGTACTTGACCGCATTATGTCACAGTCCATGAGAGAGGAATTGTCTCCATTGACATCAGGGAGGCTCTCACACAGCAGTGAACCTCTGAACAATGGTGGTTCTTTAGCAGAGACAGATAGCCTTGCAGTCTCTCCCTCCGATGAGTTCGACGACGTCGTTAAGTATTTTCGAACCCACAACAGCATACAAAACCTATTCAACGGCAGCCGTGGCAGCGCTGGAAATGGAAACAATGCCCCTAGCGGCGGAGCCAAGACGGTGGGGAGATGGCTGAAGGAGAGAAAAGAGAAGAAAAGGGAGGAGACAAGAGTGCATAATGCGCAGGTCCATGCTGCGGTTTCAGTTGCGGCGGTGGCTGCCGCTGTGGCCGCCATTGCTGCAGGCACGGCGGCTTCTTCAGGGTCAAGAAAGAACGATCAAGCAGGGAAGACAGACATGGCGGTGGCTTGTGCCGCCACGTTGGTAGCTGCTCAATGTGCTGAGGCTGCGGAGGCTATGGGAGCTGACCATGACCATCTTACTTCTGTCGTCAGCTCCGCTGTTAATGTTCGCTCTCATGATGATATTACCACTCTCACTGCAGCTGCTGCCACAGCTTTACGAGGGGCAGCGACCCTAAATGCAAGGGCAATGAAGGAGGCGTGGAACGTGTCAGCAGTGATTCCAGTGGAAAAGGGAATGGGAATCGGAGTCTGCGCTGGTAAAGCTAACAACAACAACGGTGGTGATCGCAGCAGCGGCAGCAGCTTTAGTGACGATCTTGTCCCTGTTGACCCTACGTTTCTTGGGGTCTCTAACCAAGACCTCCTTGCTAGGGGCACTGAACTCCTCAAACGCACACGAAAAGGTGACCTCCACTGGAAACTAGTGTCTGTTTACATTCACCTAACCGGACAGGTGATGCTGACAATGAAGAGCAAACACGTTGCAGGAACTTTCACCAAGAAGAAGAAGAATGTTGTGTTGGAGGTGTGCAAGAGCATGCCGGCATGGCCCGGGAGACATTTGTTCGACGACGGAGACCAGCGTCGTTACTTTGGGCTGAAGACAGAAGCGAGAGGCATTGTTGAGTTCGAGTGCAAGAACCAGAGAGAACACGACGTCTGGACTCGCGGCGTTTCGAGGCTGCTGTCTATTGTCGCCGAGGGAAAGAACAGGCATTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003002 GO:0007154 GO:0007165 GO:0007275 GO:0007389 GO:0008150 GO:0009719 GO:0009725 GO:0009733 GO:0009734 GO:0009755 GO:0009888 GO:0009987 GO:0010033 GO:0010051 GO:0010087 GO:0010305 GO:0023052 GO:0032501 GO:0032502 GO:0032870 GO:0042221 GO:0048856 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0065007 GO:0070887 GO:0071310 GO:0071365 GO:0071495

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Auxin_canalis PH_2

Protein Analysis

500

Amino Acids

53.35

Weight (kDa)

8.85

Isoelectric Point (pI)

45.06

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Auxin_canalis	PF05703	25 - 338	1.6e-106	Auxin canalisation
PH_2	PF08458	387 - 492	1.3e-41	Plant pleckstrin homology-like region

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0009825)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G63300 AT3G63300
fragaria_vesca	FvH4_2g23080
malus_domestica	MD03G1259300.v1.1 MD11G1279900.v1.1
prunus_persica	Prupe.8G235800_v2.0.a1 Prupe.8G235800_v2.0.a1
pyrus_communis	pycom03g20730 pycom11g24760
rosa_chinensis	RchiOBHm_Chr6g0290221
rosa_laevigata	RLG00000012233
rosa_multiflora	Rmu_co8342515.1_g000001 Rmu_sc0002902.1_g000011 Rmu_sc0024712.1_g000001
rosa_roxburghii	Rroxscaffold_7G00177230
rosa_rugosa	Rorug06G0211000.1
rosa_samantha	Rh6AG321300 Rh6BG328400 Rh6CG334400 Rh6DG321400
rosa_wichuraiana	Rw6G027750

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	1097
AatII	GACGTC	2 cut(s)	492, 1440
Acc16I	TGCGCA	1 cut(s)	657
Acc36I	ACCTGC	1 cut(s)	649
AccII	CGCG	1 cut(s)	1449
AcoI	YGGCCR	1 cut(s)	702
AcuI	CTGAAG	3 cut(s)	629, 717, 1400
AcyI	GRCGYC	2 cut(s)	489, 1437
AfaI	GTAC	1 cut(s)	339
AfeI	AGCGCT	1 cut(s)	553
AfiI	CCNNNNNNNGG	5 cut(s)	33, 49, 581, 670, 1150
AflIII	ACRYGT	2 cut(s)	984, 1258
AgsI	TTSAA	2 cut(s)	134, 535
AhlI	ACTAGT	1 cut(s)	1201
AjuI	GAANNNNNNNTTGG	2 cut(s)	1283, 1315
AleI	CACNNNNGTG	1 cut(s)	411
Alw26I	GTCTC	9 cut(s)	142, 156, 384, 440, 469, 632, 1132, 1330, 1350
AlwNI	CAGNNNCTG	1 cut(s)	923
Ama87I	CYCGRG	1 cut(s)	1331
Aor51HI	AGCGCT	1 cut(s)	553
AoxI	GGCC	2 cut(s)	702, 1328
AspLEI	GCGC	3 cut(s)	554, 658, 1034
AspS9I	GGNCC	2 cut(s)	661, 1329
AsuC2I	CCSGG	3 cut(s)	294, 1332, 1333
AsuHPI	GGTGA	6 cut(s)	152, 1073, 1196, 1211, 1246, 1267
AsuII	TTCGAA	1 cut(s)	505
AvaI	CYCGRG	1 cut(s)	1331
AvaII	GGWCC	1 cut(s)	661
BaeGI	GKGCMC	1 cut(s)	1157
BarI	GAAGNNNNNNTAC	1 cut(s)	29
BbsI	GAAGAC	2 cut(s)	770, 1388
BbvCI	CCTCAGC	1 cut(s)	816
BccI	CCATC	2 cut(s)	597, 858
BceAI	ACGGC	3 cut(s)	528, 553, 738
BcnI	CCSGG	3 cut(s)	294, 1332, 1333
BcoDI	GTCTC	9 cut(s)	142, 156, 384, 440, 469, 632, 1132, 1330, 1350
BcuI	ACTAGT	1 cut(s)	1201
BfaI	CTAG	3 cut(s)	576, 1149, 1202
BfmI	CTRYAG	2 cut(s)	714, 915
BfoI	RGCGCY	1 cut(s)	555
BfuAI	ACCTGC	1 cut(s)	649
BglI	GCCNNNNNGGC	2 cut(s)	701, 1327
Bme1390I	CCNGG	3 cut(s)	294, 1332, 1333
Bme18I	GGWCC	1 cut(s)	661
BmeT110I	CYCGRG	1 cut(s)	1331
BmgT120I	GGNCC	2 cut(s)	661, 1329
BmiI	GGNNCC	2 cut(s)	279, 586
BmrFI	CCNGG	3 cut(s)	294, 1332, 1333
BmsI	GCATC	1 cut(s)	1227
BoxI	GACNNNNGTC	2 cut(s)	45, 1362
BpiI	GAAGAC	2 cut(s)	770, 1388
BplI	GAGNNNNNCTC	2 cut(s)	89, 121
Bpu10I	CCTNAGC	1 cut(s)	816
Bpu14I	TTCGAA	1 cut(s)	505
BpuEI	CTTGAG	1 cut(s)	91
BpuMI	CCSGG	3 cut(s)	294, 1332, 1333
BsaHI	GRCGYC	2 cut(s)	489, 1437
BsaI	GGTCTC	2 cut(s)	1132, 1350
BsaJI	CCNNGG	4 cut(s)	292, 544, 1331, 1479
BsaWI	WCCGGW	2 cut(s)	49, 1226
BsaXI	ACNNNNNCTCC	2 cut(s)	223, 253
Bsc4I	CCNNNNNNNGG	5 cut(s)	33, 49, 581, 670, 1150
Bse118I	RCCGGY	1 cut(s)	1321
Bse1I	ACTGG	2 cut(s)	1001, 1199
Bse3DI	GCAATG	3 cut(s)	258, 708, 972
BseDI	CCNNGG	4 cut(s)	292, 544, 1331, 1479
BseLI	CCNNNNNNNGG	5 cut(s)	33, 49, 581, 670, 1150
BseMI	GCAATG	3 cut(s)	258, 708, 972
BseMII	CTCAG	2 cut(s)	167, 807
BseNI	ACTGG	2 cut(s)	1001, 1199
BseRI	GAGGAG	4 cut(s)	159, 290, 650, 1154
BseSI	GKGCMC	1 cut(s)	1157
BseYI	CCCAGC	1 cut(s)	330
Bsh1236I	CGCG	1 cut(s)	1449
BshFI	GGCC	2 cut(s)	704, 1330
BsiHKCI	CYCGRG	1 cut(s)	1331
BsiSI	CCGG	7 cut(s)	34, 50, 264, 294, 1227, 1322, 1332
BslFI	GGGAC	1 cut(s)	1085
BslI	CCNNNNNNNGG	5 cut(s)	33, 49, 581, 670, 1150
BsmAI	GTCTC	9 cut(s)	142, 156, 384, 440, 469, 632, 1132, 1330, 1350
BsmFI	GGGAC	1 cut(s)	1085
BsnI	GGCC	2 cut(s)	704, 1330
Bso31I	GGTCTC	2 cut(s)	1132, 1350
BsoBI	CYCGRG	1 cut(s)	1331
Bsp119I	TTCGAA	1 cut(s)	505
Bsp1286I	GDGCHC	1 cut(s)	1157
Bsp143I	GATC	3 cut(s)	751, 1063, 1093
BspANI	GGCC	2 cut(s)	704, 1330
BspCNI	CTCAG	2 cut(s)	166, 808
BspFNI	CGCG	1 cut(s)	1449
BspHI	TCATGA	1 cut(s)	892
BspLI	GGNNCC	2 cut(s)	279, 586
BspMAI	CTGCAG	2 cut(s)	718, 919
BspMI	ACCTGC	1 cut(s)	649
BspQI	GCTCTTC	2 cut(s)	223, 1244
BspT104I	TTCGAA	1 cut(s)	505
BspTNI	GGTCTC	2 cut(s)	1132, 1350
BsrDI	GCAATG	3 cut(s)	258, 708, 972
BsrFI	RCCGGY	1 cut(s)	1321
BsrI	ACTGG	2 cut(s)	1001, 1199
BssAI	RCCGGY	1 cut(s)	1321
BssECI	CCNNGG	4 cut(s)	292, 544, 1331, 1479
BssMI	GATC	3 cut(s)	751, 1063, 1093
BssNI	GRCGYC	2 cut(s)	489, 1437
Bst4CI	ACNGT	4 cut(s)	62, 360, 595, 1058
Bst6I	CTCTTC	2 cut(s)	223, 1244
BstACI	GRCGYC	2 cut(s)	489, 1437
BstAPI	GCANNNNNTGC	1 cut(s)	923
BstBI	TTCGAA	1 cut(s)	505
BstC8I	GCNNGC	3 cut(s)	718, 1319, 1323
BstDEI	CTNAG	2 cut(s)	153, 816
BstDSI	CCRYGG	1 cut(s)	544
BstEII	GGTNACC	1 cut(s)	1184
BstFNI	CGCG	1 cut(s)	1449
BstH2I	RGCGCY	1 cut(s)	555
BstHHI	GCGC	3 cut(s)	554, 658, 1034
BstKTI	GATC	3 cut(s)	754, 1066, 1096
BstMAI	GTCTC	9 cut(s)	142, 156, 384, 440, 469, 632, 1132, 1330, 1350
BstMBI	GATC	3 cut(s)	751, 1063, 1093
BstNSI	RCATGY	1 cut(s)	1321
BstPAI	GACNNNNGTC	2 cut(s)	45, 1362
BstPI	GGTNACC	1 cut(s)	1184
BstSCI	CCNGG	3 cut(s)	292, 1330, 1331
BstSFI	CTRYAG	2 cut(s)	714, 915
BstSLI	GKGCMC	1 cut(s)	1157
BstUI	CGCG	1 cut(s)	1449
BstV2I	GAAGAC	2 cut(s)	770, 1388
BstXI	CCANNNNNNTGG	1 cut(s)	595
BsuRI	GGCC	2 cut(s)	704, 1330
BtgI	CCRYGG	1 cut(s)	544
BtsI	GCAGTG	3 cut(s)	418, 912, 999
BtsIMutI	CAGTG	6 cut(s)	418, 912, 999, 1008, 1155, 1192
BveI	ACCTGC	1 cut(s)	649
Cac8I	GCNNGC	3 cut(s)	718, 1319, 1323
CaiI	CAGNNNCTG	1 cut(s)	923
CciI	TCATGA	1 cut(s)	892
CfoI	GCGC	3 cut(s)	554, 658, 1034
Cfr10I	RCCGGY	1 cut(s)	1321
Cfr13I	GGNCC	2 cut(s)	661, 1329
Cfr9I	CCCGGG	1 cut(s)	1331
CseI	GACGC	1 cut(s)	1352
Csp6I	GTAC	1 cut(s)	338
CspCI	CAANNNNNGTGG	2 cut(s)	1039, 1074
CviQI	GTAC	1 cut(s)	338
DdeI	CTNAG	2 cut(s)	153, 816
DpnI	GATC	3 cut(s)	753, 1065, 1095
DpnII	GATC	3 cut(s)	751, 1063, 1093
DrdI	GACNNNNNNGTC	1 cut(s)	1097
DseDI	GACNNNNNNGTC	1 cut(s)	1097
EaeI	YGGCCR	1 cut(s)	702
Eam1104I	CTCTTC	2 cut(s)	223, 1244
EarI	CTCTTC	2 cut(s)	223, 1244
EciI	GGCGGA	1 cut(s)	597
Eco31I	GGTCTC	2 cut(s)	1132, 1350
Eco47I	GGWCC	1 cut(s)	661
Eco47III	AGCGCT	1 cut(s)	553
Eco57I	CTGAAG	3 cut(s)	629, 717, 1400
Eco88I	CYCGRG	1 cut(s)	1331
Eco91I	GGTNACC	1 cut(s)	1184
EcoO65I	GGTNACC	1 cut(s)	1184
EcoT22I	ATGCAT	1 cut(s)	191
FalI	AAGNNNNNCTT	2 cut(s)	1128, 1160
FaqI	GGGAC	1 cut(s)	1085
FspBI	CTAG	3 cut(s)	576, 1149, 1202
FspI	TGCGCA	1 cut(s)	657
GlaI	GCGC	3 cut(s)	553, 657, 1033
GsaI	CCCAGC	1 cut(s)	334
HaeII	RGCGCY	1 cut(s)	555
HaeIII	GGCC	2 cut(s)	704, 1330
HapII	CCGG	7 cut(s)	34, 50, 264, 294, 1227, 1322, 1332
HgaI	GACGC	1 cut(s)	1352
HhaI	GCGC	3 cut(s)	554, 658, 1034
Hin1I	GRCGYC	2 cut(s)	489, 1437
Hin6I	GCGC	3 cut(s)	552, 656, 1032
HinP1I	GCGC	3 cut(s)	552, 656, 1032
HincII	GTYRAC	3 cut(s)	24, 55, 1108
HindII	GTYRAC	3 cut(s)	24, 55, 1108
HinfI	GANTC	6 cut(s)	8, 46, 997, 1020, 1026, 1444
HpaI	GTTAAC	1 cut(s)	24
HpaII	CCGG	7 cut(s)	34, 50, 264, 294, 1227, 1322, 1332
HphI	GGTGA	6 cut(s)	152, 1073, 1196, 1211, 1246, 1267
Hpy166II	GTNNAC	5 cut(s)	24, 55, 416, 1108, 1213
Hpy188I	TCNGA	3 cut(s)	423, 475, 1025
Hpy188III	TCNNGA	5 cut(s)	131, 251, 741, 893, 1441
Hpy8I	GTNNAC	5 cut(s)	24, 55, 416, 1108, 1213
Hpy99I	CGWCG	7 cut(s)	488, 491, 494, 1352, 1355, 1368, 1439
HpyAV	CCTTC	4 cut(s)	189, 252, 604, 964
HpyCH4III	ACNGT	4 cut(s)	62, 360, 595, 1058
HpyCH4IV	ACGT	6 cut(s)	489, 794, 984, 1115, 1260, 1437
HpyF3I	CTNAG	2 cut(s)	153, 816
HpySE526I	ACGT	6 cut(s)	489, 794, 984, 1115, 1260, 1437
Hsp92I	GRCGYC	2 cut(s)	489, 1437
HspAI	GCGC	3 cut(s)	552, 656, 1032
KroI	GCCGGC	1 cut(s)	1321
KroNI	GCCGGC	1 cut(s)	1323
KspAI	GTTAAC	1 cut(s)	24
Kzo9I	GATC	3 cut(s)	751, 1063, 1093
LguI	GCTCTTC	2 cut(s)	223, 1244
LmnI	GCTCC	5 cut(s)	172, 277, 584, 836, 875
LweI	GCATC	1 cut(s)	1227
MaeI	CTAG	3 cut(s)	576, 1149, 1202
MaeII	ACGT	6 cut(s)	489, 794, 984, 1115, 1260, 1437
MaeIII	GTNAC	6 cut(s)	62, 322, 354, 1088, 1184, 1367
MalI	GATC	3 cut(s)	753, 1065, 1095
MboI	GATC	3 cut(s)	751, 1063, 1093
MhlI	GDGCHC	1 cut(s)	1157
MluCI	AATT	3 cut(s)	196, 302, 374
MlyI	GAGTC	3 cut(s)	55, 1035, 1438
MmeI	TCCRAC	1 cut(s)	1281
Mph1103I	ATGCAT	1 cut(s)	191
MroNI	GCCGGC	1 cut(s)	1321
MseI	TTAA	3 cut(s)	23, 495, 879
MslI	CAYNNNNRTG	2 cut(s)	411, 776
MspA1I	CMGCKG	5 cut(s)	283, 698, 875, 920, 1074
MspI	CCGG	7 cut(s)	34, 50, 264, 294, 1227, 1322, 1332
MspR9I	CCNGG	3 cut(s)	294, 1332, 1333
MvnI	CGCG	1 cut(s)	1449
NaeI	GCCGGC	1 cut(s)	1323
NciI	CCSGG	3 cut(s)	294, 1332, 1333
NdeII	GATC	3 cut(s)	751, 1063, 1093
NgoMIV	GCCGGC	1 cut(s)	1321
NlaIV	GGNNCC	2 cut(s)	279, 586
NmuCI	GTSAC	5 cut(s)	62, 322, 354, 1088, 1184
NsbI	TGCGCA	1 cut(s)	657
NsiI	ATGCAT	1 cut(s)	191
NspI	RCATGY	1 cut(s)	1321
NspV	TTCGAA	1 cut(s)	505
OliI	CACNNNNGTG	1 cut(s)	411
PaeI	GCATGC	1 cut(s)	1321
PagI	TCATGA	1 cut(s)	892
PciSI	GCTCTTC	2 cut(s)	223, 1244
PcsI	WCGNNNNNNNCGW	1 cut(s)	489
PdiI	GCCGGC	1 cut(s)	1323
PfeI	GAWTC	3 cut(s)	8, 997, 1020
PflFI	GACNNNGTC	1 cut(s)	488
PleI	GAGTC	3 cut(s)	54, 1034, 1438
PpsI	GAGTC	3 cut(s)	54, 1034, 1438
PshAI	GACNNNNGTC	2 cut(s)	45, 1362
PspEI	GGTNACC	1 cut(s)	1184
PspFI	CCCAGC	1 cut(s)	330
PspN4I	GGNNCC	2 cut(s)	279, 586
PspPI	GGNCC	2 cut(s)	661, 1329
PstI	CTGCAG	2 cut(s)	718, 919
PstNI	CAGNNNCTG	1 cut(s)	923
PsyI	GACNNNGTC	1 cut(s)	488
PvuII	CAGCTG	1 cut(s)	920
RsaI	GTAC	1 cut(s)	339
RsaNI	GTAC	1 cut(s)	338
RseI	CAYNNNNRTG	2 cut(s)	411, 776
SapI	GCTCTTC	2 cut(s)	223, 1244
SaqAI	TTAA	3 cut(s)	23, 495, 879
Sau3AI	GATC	3 cut(s)	751, 1063, 1093
Sau96I	GGNCC	2 cut(s)	661, 1329
SchI	GAGTC	3 cut(s)	55, 1035, 1438
ScrFI	CCNGG	3 cut(s)	294, 1332, 1333
SduI	GDGCHC	1 cut(s)	1157
SfaNI	GCATC	1 cut(s)	1227
SfcI	CTRYAG	2 cut(s)	714, 915
SfuI	TTCGAA	1 cut(s)	505
SinI	GGWCC	1 cut(s)	661
SmaI	CCCGGG	1 cut(s)	1333
SmiMI	CAYNNNNRTG	2 cut(s)	411, 776
SmlI	CTYRAG	1 cut(s)	106
SmoI	CTYRAG	1 cut(s)	106
SpeI	ACTAGT	1 cut(s)	1201
SphI	GCATGC	1 cut(s)	1321
Sse9I	AATT	3 cut(s)	196, 302, 374
SspMI	CTAG	3 cut(s)	576, 1149, 1202
StyD4I	CCNGG	3 cut(s)	292, 1330, 1331
TaaI	ACNGT	4 cut(s)	62, 360, 595, 1058
TaiI	ACGT	6 cut(s)	492, 797, 987, 1118, 1263, 1440
TaqI	TCGA	5 cut(s)	483, 505, 1347, 1410, 1457
TasI	AATT	3 cut(s)	196, 302, 374
TauI	GCSGC	9 cut(s)	283, 582, 686, 698, 707, 728, 791, 1077, 1452
TfiI	GAWTC	3 cut(s)	8, 997, 1020
Tru1I	TTAA	3 cut(s)	23, 495, 879
Tru9I	TTAA	3 cut(s)	23, 495, 879
TscAI	CASTG	6 cut(s)	418, 919, 999, 1008, 1162, 1199
TseFI	GTSAC	5 cut(s)	62, 322, 354, 1088, 1184
Tsp45I	GTSAC	5 cut(s)	62, 322, 354, 1088, 1184
TspDTI	ATGAA	3 cut(s)	174, 983, 1262
TspGWI	ACGGA	1 cut(s)	1368
TspMI	CCCGGG	1 cut(s)	1331
TspRI	CASTG	6 cut(s)	418, 919, 999, 1008, 1162, 1199
Tth111I	GACNNNGTC	1 cut(s)	488
VpaK11BI	GGWCC	1 cut(s)	661
XceI	RCATGY	1 cut(s)	1321
XmaI	CCCGGG	1 cut(s)	1331
XspI	CTAG	3 cut(s)	576, 1149, 1202
ZraI	GACGTC	2 cut(s)	490, 1438
Zsp2I	ATGCAT	1 cut(s)	191

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.