Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr3

Orthogroup ID

OG0009825

Physical Location & Seq

Genomic Coordinates Reverse (-)

21165314 .. 21169904

Gene Structure 4591 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

pycom03g20730.6

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1512 bp

ATGGAAGAAACACTACCAATGTTAACCACTCGCCGGACTGACCAGAGTCCGGTCAACCACCGTGACCACCTCCCCAAAAGCCCAAGAGTTCCAATGGAGTTTCTCTCAAGGTCATGGAGTGCCTCTGCTCTTGAAGTCTCCAAAGCTCTGTCTCAGTCTCACCATCCTCCTCTGCCTCCTTCATGCATGGCTAATTCCAAGTCCTCAACCAACTCTTCCTCTTGCACCACCACCGCAACCACCACCACTCCTTCCATTCCTGAAGACATTGCCGAAAAAGCAGAGAAGCTGCAGGCGCTTCCCGTAAACCAATTTTACTTTGCTTCCTCTGTCACTTCCCAGCTCGTCCTTGACCGCATTATGTCACAGTCCATGAGAGAGGAATTGTCTCCATTGACATCAGGGAGGCTCTCACACAGCAGTGAACCTCTGAACAATGGTGGTTCTCTAGCAGAGACAGATAGCCTTCCAGTCTCTCCCTCCGATGAGTTCGACGACGTCGTTAAGTATTTTCGAACCCACAACAGCATACAAAACCTATTCAACGGCAGCCGTAGCAGCGCTGGAAATGGAAACAATGCCCCTAGCGGCGGAGCCAAGACGGTGGGGAGATGGCTGAAGGAGAGAAAAGAGAAGAAAAAGGAGGAGACAAGGGTGCACAATGCGCAGGTCCATGCTGCAGTTTCAGTTGCTGCGGTGGCTGCCGCTGTGGCCGCCACTGCTGCAGGCACGGCAGCTTCTTCAGGGTCAAGAAAGAACGATCAAGCAGGGAAGACAGACATGGCGGTGGCTTGTGCCGCCACGTTGGTAGCTGCTCAATGTGCTGAGGCTGCGGAGGCTATGGGAGCTGACCATGACCATCTTACTTCTGTCGTCAGCTCCGCCGTTAATGTTCGCTCTCGTGACGATATTACCACTCTCACTGCAGCTGCTGCCACAGCTTTACGAGGGGCAGCGACCCTAAAGGCAAGGGCAATGAAGGAGGCGTGGAACATATCCGCAGTGATTCCAGTGGAAAAGGGAATGGGAATCGGAGTCTGCGCTGGTAAAGCTAACAACAACAACGGTGGTGATCGCAGCAGCGGCAGCAGCAGCTTTAGTGACGATCTTGTCCCTGTTGACCCTACGTTTCTTGGGGTCTCTAACCAAGACCTCCTTGCTAGGGGCACTGAACTCCTCAAACGCACACGAAAAGGTGATCTCCACTGGAAACTAGTGTCTGTTTACATTCACCGAACCGGACAGGTGATGCTGAAAATGAAGAGCAAACACGTTGCAGGAACTTTCACCAAGAAGAAGAAGAATGTTGTGTTGGAGGTGTGCAAGAGCATGCCGGCATGGCCCGGGAGACACTTGTTCGACGACGGAGACCAGCGTCGTTACTTTGGGCTGAAGACAGAAGCGAGAGGCATTGTTGAGTTCGAGTGCAAGAACCAGAGAGAACACGACGTCTGGACTCGCGGCGTTTCGGGGCTGCTGTCTATTGTCGCCGACAGAAAGAGCAGGCATTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003002 GO:0007154 GO:0007165 GO:0007275 GO:0007389 GO:0008150 GO:0009719 GO:0009725 GO:0009733 GO:0009734 GO:0009755 GO:0009888 GO:0009987 GO:0010033 GO:0010051 GO:0010087 GO:0010305 GO:0023052 GO:0032501 GO:0032502 GO:0032870 GO:0042221 GO:0048856 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0065007 GO:0070887 GO:0071310 GO:0071365 GO:0071495

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Auxin_canalis PH_2

Protein Analysis

504

Amino Acids

53.96

Weight (kDa)

9.32

Isoelectric Point (pI)

47.17

Instability Index

Protein Domains (Pfam)

No Pfam domains detected for this protein.

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0009825)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G63300 AT3G63300
fragaria_vesca	FvH4_2g23080
malus_domestica	MD03G1259300.v1.1 MD11G1279900.v1.1
prunus_persica	Prupe.8G235800_v2.0.a1 Prupe.8G235800_v2.0.a1
pyrus_communis	pycom03g20730 pycom11g24760
rosa_chinensis	RchiOBHm_Chr6g0290221
rosa_laevigata	RLG00000012233
rosa_multiflora	Rmu_co8342515.1_g000001 Rmu_sc0002902.1_g000011 Rmu_sc0024712.1_g000001
rosa_roxburghii	Rroxscaffold_7G00177230
rosa_rugosa	Rorug06G0211000.1
rosa_samantha	Rh6AG321300 Rh6BG328400 Rh6CG334400 Rh6DG321400
rosa_wichuraiana	Rw6G027750

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	1109
AatII	GACGTC	2 cut(s)	501, 1452
Acc16I	TGCGCA	1 cut(s)	666
Acc36I	ACCTGC	1 cut(s)	658
AccII	CGCG	1 cut(s)	1461
AcoI	YGGCCR	1 cut(s)	711
AcuI	CTGAAG	4 cut(s)	282, 638, 726, 1412
AcyI	GRCGYC	2 cut(s)	498, 1449
AfeI	AGCGCT	1 cut(s)	562
AfiI	CCNNNNNNNGG	4 cut(s)	33, 49, 590, 1162
AflIII	ACRYGT	1 cut(s)	1270
AgsI	TTSAA	2 cut(s)	134, 544
AhlI	ACTAGT	1 cut(s)	1213
AjuI	GAANNNNNNNTTGG	2 cut(s)	1295, 1327
AleI	CACNNNNGTG	1 cut(s)	420
Alw21I	GWGCWC	1 cut(s)	660
Alw44I	GTGCAC	1 cut(s)	656
AlwNI	CAGNNNCTG	2 cut(s)	692, 932
Ama87I	CYCGRG	1 cut(s)	1343
Aor51HI	AGCGCT	1 cut(s)	562
AoxI	GGCC	2 cut(s)	711, 1340
ApaLI	GTGCAC	1 cut(s)	656
AspLEI	GCGC	4 cut(s)	298, 563, 667, 1043
AspS9I	GGNCC	2 cut(s)	670, 1341
AsuC2I	CCSGG	2 cut(s)	1344, 1345
AsuHPI	GGTGA	6 cut(s)	152, 1082, 1208, 1223, 1258, 1279
AsuII	TTCGAA	1 cut(s)	514
AvaI	CYCGRG	1 cut(s)	1343
AvaII	GGWCC	1 cut(s)	670
BaeGI	GKGCMC	2 cut(s)	660, 1169
BarI	GAAGNNNNNNTAC	1 cut(s)	29
BauI	CACGAG	1 cut(s)	900
BbsI	GAAGAC	3 cut(s)	270, 779, 1400
Bbv12I	GWGCWC	1 cut(s)	660
BbvCI	CCTCAGC	1 cut(s)	825
BccI	CCATC	3 cut(s)	171, 606, 867
BceAI	ACGGC	4 cut(s)	537, 562, 747, 869
BcnI	CCSGG	2 cut(s)	1344, 1345
BcuI	ACTAGT	1 cut(s)	1213
BfaI	CTAG	4 cut(s)	449, 585, 1161, 1214
BfmI	CTRYAG	4 cut(s)	290, 678, 723, 924
BfoI	RGCGCY	2 cut(s)	299, 564
BfuAI	ACCTGC	1 cut(s)	658
BglI	GCCNNNNNGGC	2 cut(s)	710, 1339
Bme1390I	CCNGG	2 cut(s)	1344, 1345
Bme18I	GGWCC	1 cut(s)	670
BmeT110I	CYCGRG	1 cut(s)	1343
BmgT120I	GGNCC	2 cut(s)	670, 1341
BmiI	GGNNCC	1 cut(s)	595
BmrFI	CCNGG	2 cut(s)	1344, 1345
BmsI	GCATC	1 cut(s)	1239
BoxI	GACNNNNGTC	2 cut(s)	45, 1374
BpiI	GAAGAC	3 cut(s)	270, 779, 1400
BplI	GAGNNNNNCTC	2 cut(s)	89, 121
Bpu10I	CCTNAGC	1 cut(s)	825
Bpu14I	TTCGAA	1 cut(s)	514
BpuEI	CTTGAG	1 cut(s)	91
BpuMI	CCSGG	2 cut(s)	1344, 1345
BsaHI	GRCGYC	2 cut(s)	498, 1449
BsaI	GGTCTC	2 cut(s)	1144, 1362
BsaJI	CCNNGG	1 cut(s)	1343
BsaWI	WCCGGW	2 cut(s)	49, 1238
BsaXI	ACNNNNNCTCC	2 cut(s)	232, 262
Bsc4I	CCNNNNNNNGG	4 cut(s)	33, 49, 590, 1162
Bse118I	RCCGGY	1 cut(s)	1333
Bse1I	ACTGG	3 cut(s)	470, 1010, 1211
Bse3DI	GCAATG	2 cut(s)	267, 981
BseDI	CCNNGG	1 cut(s)	1343
BseGI	GGATG	1 cut(s)	163
BseLI	CCNNNNNNNGG	4 cut(s)	33, 49, 590, 1162
BseMI	GCAATG	2 cut(s)	267, 981
BseMII	CTCAG	2 cut(s)	167, 816
BseNI	ACTGG	3 cut(s)	470, 1010, 1211
BseRI	GAGGAG	3 cut(s)	159, 659, 1166
BseSI	GKGCMC	2 cut(s)	660, 1169
BseYI	CCCAGC	1 cut(s)	339
Bsh1236I	CGCG	1 cut(s)	1461
BshFI	GGCC	2 cut(s)	713, 1342
BsiHKAI	GWGCWC	1 cut(s)	660
BsiHKCI	CYCGRG	1 cut(s)	1343
BsiSI	CCGG	5 cut(s)	34, 50, 1239, 1334, 1344
BslFI	GGGAC	1 cut(s)	1097
BslI	CCNNNNNNNGG	4 cut(s)	33, 49, 590, 1162
BsmFI	GGGAC	1 cut(s)	1097
BsnI	GGCC	2 cut(s)	713, 1342
Bso31I	GGTCTC	2 cut(s)	1144, 1362
BsoBI	CYCGRG	1 cut(s)	1343
Bsp119I	TTCGAA	1 cut(s)	514
Bsp1286I	GDGCHC	2 cut(s)	660, 1169
Bsp143I	GATC	4 cut(s)	760, 1072, 1105, 1198
BspANI	GGCC	2 cut(s)	713, 1342
BspCNI	CTCAG	2 cut(s)	166, 817
BspFNI	CGCG	1 cut(s)	1461
BspLI	GGNNCC	1 cut(s)	595
BspMAI	CTGCAG	4 cut(s)	294, 682, 727, 928
BspMI	ACCTGC	1 cut(s)	658
BspQI	GCTCTTC	1 cut(s)	1256
BspT104I	TTCGAA	1 cut(s)	514
BspTNI	GGTCTC	2 cut(s)	1144, 1362
BsrDI	GCAATG	2 cut(s)	267, 981
BsrFI	RCCGGY	1 cut(s)	1333
BsrI	ACTGG	3 cut(s)	470, 1010, 1211
BssAI	RCCGGY	1 cut(s)	1333
BssECI	CCNNGG	1 cut(s)	1343
BssMI	GATC	4 cut(s)	760, 1072, 1105, 1198
BssNI	GRCGYC	2 cut(s)	498, 1449
BssSI	CACGAG	1 cut(s)	900
Bst2BI	CACGAG	1 cut(s)	900
Bst4CI	ACNGT	4 cut(s)	62, 369, 604, 1067
Bst6I	CTCTTC	2 cut(s)	220, 1256
BstACI	GRCGYC	2 cut(s)	498, 1449
BstAPI	GCANNNNNTGC	1 cut(s)	932
BstBI	TTCGAA	1 cut(s)	514
BstC8I	GCNNGC	5 cut(s)	294, 727, 1331, 1335, 1505
BstDEI	CTNAG	2 cut(s)	153, 825
BstF5I	GGATG	1 cut(s)	163
BstFNI	CGCG	1 cut(s)	1461
BstH2I	RGCGCY	2 cut(s)	299, 564
BstHHI	GCGC	4 cut(s)	298, 563, 667, 1043
BstKTI	GATC	4 cut(s)	763, 1075, 1108, 1201
BstMBI	GATC	4 cut(s)	760, 1072, 1105, 1198
BstNSI	RCATGY	1 cut(s)	1333
BstPAI	GACNNNNGTC	2 cut(s)	45, 1374
BstSCI	CCNGG	2 cut(s)	1342, 1343
BstSFI	CTRYAG	4 cut(s)	290, 678, 723, 924
BstSLI	GKGCMC	2 cut(s)	660, 1169
BstUI	CGCG	1 cut(s)	1461
BstV2I	GAAGAC	3 cut(s)	270, 779, 1400
BstXI	CCANNNNNNTGG	1 cut(s)	604
BsuRI	GGCC	2 cut(s)	713, 1342
BtsCI	GGATG	1 cut(s)	163
BtsI	GCAGTG	4 cut(s)	427, 717, 921, 1008
BtsIMutI	CAGTG	7 cut(s)	427, 717, 921, 1008, 1017, 1167, 1204
BveI	ACCTGC	1 cut(s)	658
Cac8I	GCNNGC	5 cut(s)	294, 727, 1331, 1335, 1505
CaiI	CAGNNNCTG	2 cut(s)	692, 932
CfoI	GCGC	4 cut(s)	298, 563, 667, 1043
Cfr10I	RCCGGY	1 cut(s)	1333
Cfr13I	GGNCC	2 cut(s)	670, 1341
Cfr9I	CCCGGG	1 cut(s)	1343
CseI	GACGC	1 cut(s)	1364
CspCI	CAANNNNNGTGG	2 cut(s)	1048, 1083
CviAII	CATG	9 cut(s)	114, 183, 187, 373, 674, 781, 854, 1330, 1338
DdeI	CTNAG	2 cut(s)	153, 825
DpnI	GATC	4 cut(s)	762, 1074, 1107, 1200
DpnII	GATC	4 cut(s)	760, 1072, 1105, 1198
DrdI	GACNNNNNNGTC	1 cut(s)	1109
DseDI	GACNNNNNNGTC	1 cut(s)	1109
EaeI	YGGCCR	1 cut(s)	711
Eam1104I	CTCTTC	2 cut(s)	220, 1256
EarI	CTCTTC	2 cut(s)	220, 1256
EciI	GGCGGA	2 cut(s)	606, 871
Eco31I	GGTCTC	2 cut(s)	1144, 1362
Eco47I	GGWCC	1 cut(s)	670
Eco47III	AGCGCT	1 cut(s)	562
Eco57I	CTGAAG	4 cut(s)	282, 638, 726, 1412
Eco88I	CYCGRG	1 cut(s)	1343
EcoT22I	ATGCAT	1 cut(s)	188
FaeI	CATG	9 cut(s)	117, 186, 190, 376, 677, 784, 857, 1333, 1341
FalI	AAGNNNNNCTT	2 cut(s)	1140, 1172
FaqI	GGGAC	1 cut(s)	1097
FatI	CATG	9 cut(s)	113, 182, 186, 372, 673, 780, 853, 1329, 1337
FokI	GGATG	1 cut(s)	150
FspBI	CTAG	4 cut(s)	449, 585, 1161, 1214
FspI	TGCGCA	1 cut(s)	666
GlaI	GCGC	4 cut(s)	297, 562, 666, 1042
GsaI	CCCAGC	1 cut(s)	343
HaeII	RGCGCY	2 cut(s)	299, 564
HaeIII	GGCC	2 cut(s)	713, 1342
HapII	CCGG	5 cut(s)	34, 50, 1239, 1334, 1344
HgaI	GACGC	1 cut(s)	1364
HhaI	GCGC	4 cut(s)	298, 563, 667, 1043
Hin1I	GRCGYC	2 cut(s)	498, 1449
Hin1II	CATG	9 cut(s)	117, 186, 190, 376, 677, 784, 857, 1333, 1341
Hin6I	GCGC	4 cut(s)	296, 561, 665, 1041
HinP1I	GCGC	4 cut(s)	296, 561, 665, 1041
HincII	GTYRAC	3 cut(s)	24, 55, 1120
HindII	GTYRAC	3 cut(s)	24, 55, 1120
HinfI	GANTC	5 cut(s)	46, 1006, 1029, 1035, 1456
HpaI	GTTAAC	1 cut(s)	24
HpaII	CCGG	5 cut(s)	34, 50, 1239, 1334, 1344
HphI	GGTGA	6 cut(s)	152, 1082, 1208, 1223, 1258, 1279
Hpy166II	GTNNAC	7 cut(s)	24, 55, 307, 425, 658, 1120, 1225
Hpy188I	TCNGA	3 cut(s)	432, 484, 1034
Hpy188III	TCNNGA	5 cut(s)	131, 260, 750, 902, 1453
Hpy8I	GTNNAC	7 cut(s)	24, 55, 307, 425, 658, 1120, 1225
Hpy99I	CGWCG	7 cut(s)	497, 500, 503, 1364, 1367, 1380, 1451
HpyAV	CCTTC	5 cut(s)	189, 261, 476, 613, 973
HpyCH4III	ACNGT	4 cut(s)	62, 369, 604, 1067
HpyCH4IV	ACGT	5 cut(s)	498, 803, 1127, 1272, 1449
HpyF3I	CTNAG	2 cut(s)	153, 825
HpySE526I	ACGT	5 cut(s)	498, 803, 1127, 1272, 1449
Hsp92I	GRCGYC	2 cut(s)	498, 1449
Hsp92II	CATG	9 cut(s)	117, 186, 190, 376, 677, 784, 857, 1333, 1341
HspAI	GCGC	4 cut(s)	296, 561, 665, 1041
KroI	GCCGGC	1 cut(s)	1333
KroNI	GCCGGC	1 cut(s)	1335
KspAI	GTTAAC	1 cut(s)	24
Kzo9I	GATC	4 cut(s)	760, 1072, 1105, 1198
LguI	GCTCTTC	1 cut(s)	1256
LmnI	GCTCC	3 cut(s)	593, 845, 884
LweI	GCATC	1 cut(s)	1239
MaeI	CTAG	4 cut(s)	449, 585, 1161, 1214
MaeII	ACGT	5 cut(s)	498, 803, 1127, 1272, 1449
MaeIII	GTNAC	6 cut(s)	62, 331, 363, 902, 1100, 1379
MalI	GATC	4 cut(s)	762, 1074, 1107, 1200
MboI	GATC	4 cut(s)	760, 1072, 1105, 1198
MhlI	GDGCHC	2 cut(s)	660, 1169
MluCI	AATT	3 cut(s)	193, 311, 383
MlyI	GAGTC	3 cut(s)	55, 1044, 1450
MmeI	TCCRAC	1 cut(s)	1293
Mph1103I	ATGCAT	1 cut(s)	188
MroNI	GCCGGC	1 cut(s)	1333
MseI	TTAA	3 cut(s)	23, 504, 888
MslI	CAYNNNNRTG	2 cut(s)	420, 785
MspA1I	CMGCKG	3 cut(s)	707, 929, 1083
MspI	CCGG	5 cut(s)	34, 50, 1239, 1334, 1344
MspR9I	CCNGG	2 cut(s)	1344, 1345
MvnI	CGCG	1 cut(s)	1461
NaeI	GCCGGC	1 cut(s)	1335
NciI	CCSGG	2 cut(s)	1344, 1345
NdeII	GATC	4 cut(s)	760, 1072, 1105, 1198
NgoMIV	GCCGGC	1 cut(s)	1333
NlaIII	CATG	9 cut(s)	117, 186, 190, 376, 677, 784, 857, 1333, 1341
NlaIV	GGNNCC	1 cut(s)	595
NmuCI	GTSAC	5 cut(s)	62, 331, 363, 902, 1100
NsbI	TGCGCA	1 cut(s)	666
NsiI	ATGCAT	1 cut(s)	188
NspI	RCATGY	1 cut(s)	1333
NspV	TTCGAA	1 cut(s)	514
OliI	CACNNNNGTG	1 cut(s)	420
PaeI	GCATGC	1 cut(s)	1333
PciSI	GCTCTTC	1 cut(s)	1256
PcsI	WCGNNNNNNNCGW	1 cut(s)	498
PdiI	GCCGGC	1 cut(s)	1335
PfeI	GAWTC	2 cut(s)	1006, 1029
PflFI	GACNNNGTC	1 cut(s)	497
PleI	GAGTC	3 cut(s)	54, 1043, 1450
PpsI	GAGTC	3 cut(s)	54, 1043, 1450
PshAI	GACNNNNGTC	2 cut(s)	45, 1374
PspFI	CCCAGC	1 cut(s)	339
PspN4I	GGNNCC	1 cut(s)	595
PspPI	GGNCC	2 cut(s)	670, 1341
PstI	CTGCAG	4 cut(s)	294, 682, 727, 928
PstNI	CAGNNNCTG	2 cut(s)	692, 932
PsyI	GACNNNGTC	1 cut(s)	497
PvuII	CAGCTG	1 cut(s)	929
RseI	CAYNNNNRTG	2 cut(s)	420, 785
SapI	GCTCTTC	1 cut(s)	1256
SaqAI	TTAA	3 cut(s)	23, 504, 888
Sau3AI	GATC	4 cut(s)	760, 1072, 1105, 1198
Sau96I	GGNCC	2 cut(s)	670, 1341
SchI	GAGTC	3 cut(s)	55, 1044, 1450
ScrFI	CCNGG	2 cut(s)	1344, 1345
SduI	GDGCHC	2 cut(s)	660, 1169
SfaNI	GCATC	1 cut(s)	1239
SfcI	CTRYAG	4 cut(s)	290, 678, 723, 924
SfuI	TTCGAA	1 cut(s)	514
SinI	GGWCC	1 cut(s)	670
SmaI	CCCGGG	1 cut(s)	1345
SmiMI	CAYNNNNRTG	2 cut(s)	420, 785
SmlI	CTYRAG	1 cut(s)	106
SmoI	CTYRAG	1 cut(s)	106
SpeI	ACTAGT	1 cut(s)	1213
SphI	GCATGC	1 cut(s)	1333
Sse9I	AATT	3 cut(s)	193, 311, 383
SspMI	CTAG	4 cut(s)	449, 585, 1161, 1214
StyD4I	CCNGG	2 cut(s)	1342, 1343
TaaI	ACNGT	4 cut(s)	62, 369, 604, 1067
TaiI	ACGT	5 cut(s)	501, 806, 1130, 1275, 1452
TaqI	TCGA	4 cut(s)	492, 514, 1359, 1422
TasI	AATT	3 cut(s)	193, 311, 383
TauI	GCSGC	6 cut(s)	591, 707, 716, 800, 1086, 1464
TfiI	GAWTC	2 cut(s)	1006, 1029
Tru1I	TTAA	3 cut(s)	23, 504, 888
Tru9I	TTAA	3 cut(s)	23, 504, 888
TscAI	CASTG	7 cut(s)	427, 724, 928, 1008, 1017, 1174, 1211
TseFI	GTSAC	5 cut(s)	62, 331, 363, 902, 1100
Tsp45I	GTSAC	5 cut(s)	62, 331, 363, 902, 1100
TspDTI	ATGAA	3 cut(s)	171, 992, 1274
TspGWI	ACGGA	1 cut(s)	1380
TspMI	CCCGGG	1 cut(s)	1343
TspRI	CASTG	7 cut(s)	427, 724, 928, 1008, 1017, 1174, 1211
Tth111I	GACNNNGTC	1 cut(s)	497
VneI	GTGCAC	1 cut(s)	656
VpaK11BI	GGWCC	1 cut(s)	670
XceI	RCATGY	1 cut(s)	1333
XmaI	CCCGGG	1 cut(s)	1343
XspI	CTAG	4 cut(s)	449, 585, 1161, 1214
ZraI	GACGTC	2 cut(s)	499, 1450
Zsp2I	ATGCAT	1 cut(s)	188

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.