MD03G1269800.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0012133
Physical Location & Seq
Forward (+)
35397492 .. 35401951
4460 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1269800.v1.1.491

Sequence Viewer

Length: 1254 bp
ATGGCGGATAAGGTTTGCCTGATGGCGTTTCATGTTTACCCTCACGGCCTCGTTCTGAACCAGGAGCAACCCATGAGCTCTATCAAGGCTTTCCAATCATTTATGCCAAGTTTTGGAGCAAAGGAGGAAATCACAAAGTTAGGATCTTTGAAATTGATGTCACAGCAATGCGAGGAGCCATGGAAACTCAAAAGTGGAGTGCCGGAGGCCAACTGGTTTGTAAAGACTGTCTCAGAAATTGAAAGACCTGCTCTGAATGTGCAAGATGTTCATCCGGACTCATTGCTGTTCAGCTGTGGAATTGCCGAACATTTCATAAGACGTGAAAAAATGTTGCAGTTCCTCAGATCAGGAGAAAGTGAAGTGGAAAGAGGCGGAGTAGATATAACCTTACTATATGACTTGATGGGGTTACATGAAATGGGCCAACAACCATCAATGCCTTCTCTTATCTACCCGAGTAGGGAGTTGAATACCCAGAAGCCCCTGTTGGACTTTGTAGGCAGCCCTCACTGGAGTTCAAAAGTTACAGTTCAGCCAGATGGTCGAGTCTTATTCACAGGTTCCGAGGCTGAGATGAAGCATCTTCTTTCTGTTGTTGCTGAATTTTACTCCTTGAGAAATACGGTTGTTTGGAAAAAGCAGTCTCTGTTGGTTCCGCACTTTAAGAGTGTGGGGAGCCAGGAAGCAGGAGTTATTATTGATGGAACTTCGTTCAATATGCAAGCGACTACCCTTGCTCCCTTAAAGAGTCCTGAAAAAGTTAAGACGAAGGCGAAGCCAAAGCAGAAGAGTGGTAAAAAAGCGGGCAAAGATAGAGATCTCTACAAGAGGAACTATTTCCACGCCTGTGAGAGCCTTCTATCCATAATGATGAGCAGAAAGCAGAATGGGAAATTGGCAATCCTCTCACTTAAAAGATCTGGCCCCGAGCTTCCGGATCTCCTGACCCAGTTTTCTGCCGCTATTGCTGGAACTGGCTTGGCTGTCCTCTTCTCTGTCCTCTGTAAAGTAGCTTCTGGGAGGGTACCCTTTTGTGCGTCCAAACTCTTGAATACTGGAGTTGCATTGGCATTAGTTTGGCTCTCTTGGGGGGTGCACAAACTGAGGGACACAATCGTTGAAACCAGCAAGAATTCAAAGAAGCGGGCTTTAAAAGAAGAGGAAATCATGGAAAGGGTAGATAGAAGCGTGAAGGAGATTTACTTGAGAGCGGGAACATTGATGGCAGTGGCGGTGTTGAGGCTTGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

418

Amino Acids

46.41

Weight (kDa)

9.44

Isoelectric Point (pI)

47.74

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012133)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G03340 AT4G02920 AT4G02920
fragaria_vesca FvH4_2g24050
malus_domestica MD03G1269800.v1.1 MD11G1290800.v1.1
prunus_persica Prupe.8G245900_v2.0.a1
pyrus_communis pycom03g21510
rosa_chinensis RchiOBHm_Chr6g0291821
rosa_laevigata RLG00000012085
rosa_multiflora Rmu_sc0001043.1_g000015
rosa_roxburghii Rroxscaffold_7G00175720
rosa_rugosa Rorug06G0221600
rosa_samantha Rh6AG334400 Rh6BG341900 Rh6CG347800 Rh6DG334700
rosa_wichuraiana Rw6G028950 Rw6G029110

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 256
Acc65I GGTACC 1 cut(s) 1027
AccB1I GGYRCC 1 cut(s) 1027
AccB7I CCANNNNNTGG 1 cut(s) 113
AccBSI CCGCTC 1 cut(s) 1214
AccIII TCCGGA 2 cut(s) 274, 937
AciI CCGC 8 cut(s) 5, 375, 659, 806, 963, 1147, 1214, 1235
AclWI GGATC 2 cut(s) 151, 948
AcsI RAATTY 2 cut(s) 605, 1135
AfaI GTAC 1 cut(s) 1029
AfiI CCNNNNNNNGG 2 cut(s) 113, 463
AgsI TTSAA 8 cut(s) 151, 242, 472, 522, 718, 1054, 1124, 1140
AjiI CACGTC 1 cut(s) 323
AjnI CCWGG 2 cut(s) 60, 681
AjuI GAANNNNNNNTTGG 4 cut(s) 473, 505, 881, 913
AleI CACNNNNGTG 1 cut(s) 849
AluBI AGCT 4 cut(s) 78, 294, 934, 1016
AluI AGCT 4 cut(s) 78, 294, 934, 1016
Alw21I GWGCWC 2 cut(s) 80, 1101
Alw26I GTCTC 2 cut(s) 235, 651
Alw44I GTGCAC 1 cut(s) 1097
AlwI GGATC 2 cut(s) 151, 948
AlwNI CAGNNNCTG 1 cut(s) 649
Ama87I CYCGRG 2 cut(s) 457, 929
Aor13HI TCCGGA 2 cut(s) 274, 937
AoxI GGCC 4 cut(s) 46, 207, 424, 925
ApaLI GTGCAC 1 cut(s) 1097
ApeKI GCWGC 1 cut(s) 504
ApoI RAATTY 2 cut(s) 605, 1135
Asp700I GAANNNNTTC 1 cut(s) 839
Asp718I GGTACC 1 cut(s) 1027
AspS9I GGNCC 2 cut(s) 424, 926
AvaI CYCGRG 2 cut(s) 457, 929
BaeGI GKGCMC 1 cut(s) 1101
BanI GGYRCC 1 cut(s) 1027
BanII GRGCYC 1 cut(s) 80
BarI GAAGNNNNNNTAC 4 cut(s) 1000, 1032, 1187, 1219
Bbv12I GWGCWC 2 cut(s) 80, 1101
BbvI GCAGC 1 cut(s) 516
BccI CCATC 6 cut(s) 16, 400, 442, 536, 698, 1219
BceAI ACGGC 1 cut(s) 61
BciT130I CCWGG 2 cut(s) 62, 683
BcoDI GTCTC 2 cut(s) 235, 651
BfuAI ACCTGC 1 cut(s) 256
BglII AGATCT 2 cut(s) 820, 920
BisI GCNGC 2 cut(s) 505, 963
BlsI GCNGC 2 cut(s) 506, 964
Bme1390I CCNGG 2 cut(s) 62, 683
BmeT110I CYCGRG 2 cut(s) 457, 929
BmgBI CACGTC 1 cut(s) 323
BmgT120I GGNCC 2 cut(s) 424, 926
BmiI GGNNCC 6 cut(s) 177, 565, 657, 680, 928, 1029
BmrFI CCNGG 2 cut(s) 62, 683
BmrI ACTGGG 1 cut(s) 946
BmsI GCATC 1 cut(s) 592
BmuI ACTGGG 1 cut(s) 946
BpmI CTGGAG 2 cut(s) 535, 1080
BpuEI CTTGAG 2 cut(s) 637, 1228
BsaBI GATNNNNATC 2 cut(s) 270, 819
BsaJI CCNNGG 2 cut(s) 179, 567
BsaWI WCCGGW 2 cut(s) 274, 937
BsaXI ACNNNNNCTCC 2 cut(s) 724, 754
Bsc4I CCNNNNNNNGG 2 cut(s) 113, 463
Bse1I ACTGG 5 cut(s) 218, 518, 952, 982, 1063
Bse3DI GCAATG 2 cut(s) 173, 281
Bse8I GATNNNNATC 2 cut(s) 270, 819
BseAI TCCGGA 2 cut(s) 274, 937
BseBI CCWGG 2 cut(s) 62, 683
BseDI CCNNGG 2 cut(s) 179, 567
BseGI GGATG 1 cut(s) 271
BseJI GATNNNNATC 2 cut(s) 270, 819
BseLI CCNNNNNNNGG 2 cut(s) 113, 463
BseMI GCAATG 2 cut(s) 173, 281
BseMII CTCAG 4 cut(s) 246, 358, 564, 1097
BseNI ACTGG 5 cut(s) 218, 518, 952, 982, 1063
BseRI GAGGAG 1 cut(s) 188
BseSI GKGCMC 1 cut(s) 1101
BseXI GCAGC 1 cut(s) 516
BshFI GGCC 4 cut(s) 48, 209, 426, 927
BshNI GGYRCC 1 cut(s) 1027
BsiHKAI GWGCWC 2 cut(s) 80, 1101
BsiHKCI CYCGRG 2 cut(s) 457, 929
BsiSI CCGG 3 cut(s) 203, 275, 938
BslFI GGGAC 1 cut(s) 1124
BslI CCNNNNNNNGG 2 cut(s) 113, 463
BsmAI GTCTC 2 cut(s) 235, 651
BsmFI GGGAC 1 cut(s) 1124
BsnI GGCC 4 cut(s) 48, 209, 426, 927
BsoBI CYCGRG 2 cut(s) 457, 929
Bsp1286I GDGCHC 2 cut(s) 80, 1101
Bsp13I TCCGGA 2 cut(s) 274, 937
Bsp143I GATC 5 cut(s) 143, 347, 820, 920, 940
Bsp19I CCATGG 1 cut(s) 179
BspACI CCGC 8 cut(s) 5, 375, 659, 806, 963, 1147, 1214, 1235
BspANI GGCC 4 cut(s) 48, 209, 426, 927
BspCNI CTCAG 4 cut(s) 245, 357, 565, 1098
BspEI TCCGGA 2 cut(s) 274, 937
BspLI GGNNCC 6 cut(s) 177, 565, 657, 680, 928, 1029
BspMI ACCTGC 1 cut(s) 256
BspPI GGATC 2 cut(s) 151, 948
BspT107I GGYRCC 1 cut(s) 1027
BsrBI CCGCTC 1 cut(s) 1214
BsrDI GCAATG 2 cut(s) 173, 281
BsrI ACTGG 5 cut(s) 218, 518, 952, 982, 1063
BssECI CCNNGG 2 cut(s) 179, 567
BssMI GATC 5 cut(s) 143, 347, 820, 920, 940
BssT1I CCWWGG 1 cut(s) 179
Bst2UI CCWGG 2 cut(s) 62, 683
Bst4CI ACNGT 3 cut(s) 229, 532, 628
Bst6I CTCTTC 3 cut(s) 785, 998, 1155
BstC8I GCNNGC 4 cut(s) 726, 808, 1149, 1248
BstDEI CTNAG 4 cut(s) 232, 344, 573, 1106
BstDSI CCRYGG 1 cut(s) 179
BstF5I GGATG 1 cut(s) 271
BstKTI GATC 5 cut(s) 146, 350, 823, 923, 943
BstMAI GTCTC 2 cut(s) 235, 651
BstMBI GATC 5 cut(s) 143, 347, 820, 920, 940
BstMWI GCNNNNNNNGC 1 cut(s) 968
BstNI CCWGG 2 cut(s) 62, 683
BstSCI CCNGG 2 cut(s) 60, 681
BstSLI GKGCMC 1 cut(s) 1101
BstV1I GCAGC 1 cut(s) 516
BstX2I RGATCY 4 cut(s) 143, 820, 920, 940
BstYI RGATCY 4 cut(s) 143, 820, 920, 940
BsuRI GGCC 4 cut(s) 48, 209, 426, 927
BtgI CCRYGG 1 cut(s) 179
BtrI CACGTC 1 cut(s) 323
BtsCI GGATG 1 cut(s) 271
BtsI GCAGTG 1 cut(s) 1236
BtsIMutI CAGTG 2 cut(s) 511, 1236
BveI ACCTGC 1 cut(s) 256
Cac8I GCNNGC 4 cut(s) 726, 808, 1149, 1248
CaiI CAGNNNCTG 1 cut(s) 649
Cfr13I GGNCC 2 cut(s) 424, 926
CseI GACGC 1 cut(s) 1029
Csp6I GTAC 1 cut(s) 1028
CviAII CATG 5 cut(s) 32, 73, 180, 416, 1171
CviQI GTAC 1 cut(s) 1028
DdeI CTNAG 4 cut(s) 232, 344, 573, 1106
DpnI GATC 5 cut(s) 145, 349, 822, 922, 942
DpnII GATC 5 cut(s) 143, 347, 820, 920, 940
DraI TTTAAA 1 cut(s) 1155
Eam1104I CTCTTC 3 cut(s) 785, 998, 1155
EarI CTCTTC 3 cut(s) 785, 998, 1155
EciI GGCGGA 2 cut(s) 20, 390
Ecl136II GAGCTC 1 cut(s) 78
Eco130I CCWWGG 1 cut(s) 179
Eco24I GRGCYC 1 cut(s) 80
Eco53kI GAGCTC 1 cut(s) 78
Eco88I CYCGRG 2 cut(s) 457, 929
EcoICRI GAGCTC 1 cut(s) 78
EcoRI GAATTC 1 cut(s) 1135
EcoRII CCWGG 2 cut(s) 60, 681
EcoT14I CCWWGG 1 cut(s) 179
EcoT38I GRGCYC 1 cut(s) 80
ErhI CCWWGG 1 cut(s) 179
FaeI CATG 5 cut(s) 35, 76, 183, 419, 1174
FaqI GGGAC 1 cut(s) 1124
FatI CATG 5 cut(s) 31, 72, 179, 415, 1170
FauI CCCGC 3 cut(s) 799, 1140, 1207
Fnu4HI GCNGC 2 cut(s) 505, 963
FokI GGATG 1 cut(s) 258
FriOI GRGCYC 1 cut(s) 80
Fsp4HI GCNGC 2 cut(s) 505, 963
GluI GCNGC 2 cut(s) 505, 963
GsuI CTGGAG 2 cut(s) 535, 1080
HaeIII GGCC 4 cut(s) 48, 209, 426, 927
HapII CCGG 3 cut(s) 203, 275, 938
HgaI GACGC 1 cut(s) 1029
Hin1II CATG 5 cut(s) 35, 76, 183, 419, 1174
HinfI GANTC 3 cut(s) 278, 549, 751
HpaII CCGG 3 cut(s) 203, 275, 938
Hpy166II GTNNAC 2 cut(s) 37, 1099
Hpy188I TCNGA 5 cut(s) 57, 235, 255, 347, 568
Hpy188III TCNNGA 6 cut(s) 275, 351, 755, 938, 946, 1051
Hpy8I GTNNAC 2 cut(s) 37, 1099
HpyAV CCTTC 4 cut(s) 453, 766, 869, 1189
HpyCH4III ACNGT 3 cut(s) 229, 532, 628
HpyCH4IV ACGT 1 cut(s) 322
HpyCH4V TGCA 5 cut(s) 262, 337, 724, 1067, 1099
HpyF10VI GCNNNNNNNGC 1 cut(s) 968
HpyF3I CTNAG 4 cut(s) 232, 344, 573, 1106
HpySE526I ACGT 1 cut(s) 322
Hsp92II CATG 5 cut(s) 35, 76, 183, 419, 1174
Kpn2I TCCGGA 2 cut(s) 274, 937
KpnI GGTACC 1 cut(s) 1031
Kzo9I GATC 5 cut(s) 143, 347, 820, 920, 940
LmnI GCTCC 5 cut(s) 64, 116, 175, 678, 745
Lsp1109I GCAGC 1 cut(s) 516
LweI GCATC 1 cut(s) 592
MaeII ACGT 1 cut(s) 322
MaeIII GTNAC 3 cut(s) 159, 411, 526
MalI GATC 5 cut(s) 145, 349, 822, 922, 942
MbiI CCGCTC 1 cut(s) 1214
MboI GATC 5 cut(s) 143, 347, 820, 920, 940
MboII GAAGA 4 cut(s) 578, 802, 985, 1172
MflI RGATCY 4 cut(s) 143, 820, 920, 940
MhlI GDGCHC 2 cut(s) 80, 1101
MluCI AATT 6 cut(s) 152, 237, 300, 605, 896, 1135
MlyI GAGTC 3 cut(s) 272, 558, 760
MmeI TCCRAC 1 cut(s) 471
MroI TCCGGA 2 cut(s) 274, 937
MroXI GAANNNNTTC 1 cut(s) 839
MseI TTAA 5 cut(s) 666, 746, 765, 915, 1154
MslI CAYNNNNRTG 3 cut(s) 166, 849, 872
MspA1I CMGCKG 1 cut(s) 294
MspI CCGG 3 cut(s) 203, 275, 938
MspR9I CCNGG 2 cut(s) 62, 683
MvaI CCWGG 2 cut(s) 62, 683
MwoI GCNNNNNNNGC 1 cut(s) 968
NcoI CCATGG 1 cut(s) 179
NdeII GATC 5 cut(s) 143, 347, 820, 920, 940
NlaIII CATG 5 cut(s) 35, 76, 183, 419, 1174
NlaIV GGNNCC 6 cut(s) 177, 565, 657, 680, 928, 1029
NmuCI GTSAC 1 cut(s) 159
OliI CACNNNNGTG 1 cut(s) 849
PdmI GAANNNNTTC 1 cut(s) 839
PflMI CCANNNNNTGG 1 cut(s) 113
PkrI GCNGC 2 cut(s) 506, 964
PleI GAGTC 3 cut(s) 272, 557, 759
PpsI GAGTC 3 cut(s) 272, 557, 759
Psp124BI GAGCTC 1 cut(s) 80
Psp6I CCWGG 2 cut(s) 60, 681
PspGI CCWGG 2 cut(s) 60, 681
PspN4I GGNNCC 6 cut(s) 177, 565, 657, 680, 928, 1029
PspPI GGNCC 2 cut(s) 424, 926
PstNI CAGNNNCTG 1 cut(s) 649
PsuI RGATCY 4 cut(s) 143, 820, 920, 940
PvuII CAGCTG 1 cut(s) 294
RsaI GTAC 1 cut(s) 1029
RsaNI GTAC 1 cut(s) 1028
RseI CAYNNNNRTG 3 cut(s) 166, 849, 872
SacI GAGCTC 1 cut(s) 80
SaqAI TTAA 5 cut(s) 666, 746, 765, 915, 1154
SatI GCNGC 2 cut(s) 505, 963
Sau3AI GATC 5 cut(s) 143, 347, 820, 920, 940
Sau96I GGNCC 2 cut(s) 424, 926
SchI GAGTC 3 cut(s) 272, 558, 760
ScrFI CCNGG 2 cut(s) 62, 683
SduI GDGCHC 2 cut(s) 80, 1101
SetI ASST 9 cut(s) 15, 80, 250, 296, 325, 392, 565, 936, 1018
SfaNI GCATC 1 cut(s) 592
SmiMI CAYNNNNRTG 3 cut(s) 166, 849, 872
SmlI CTYRAG 2 cut(s) 616, 1207
SmoI CTYRAG 2 cut(s) 616, 1207
Sse9I AATT 6 cut(s) 152, 237, 300, 605, 896, 1135
SsiI CCGC 8 cut(s) 5, 375, 659, 806, 963, 1147, 1214, 1235
SstI GAGCTC 1 cut(s) 80
StyD4I CCNGG 2 cut(s) 60, 681
StyI CCWWGG 1 cut(s) 179
TaaI ACNGT 3 cut(s) 229, 532, 628
TaiI ACGT 1 cut(s) 325
TaqI TCGA 1 cut(s) 547
TasI AATT 6 cut(s) 152, 237, 300, 605, 896, 1135
TauI GCSGC 1 cut(s) 965
Tru1I TTAA 5 cut(s) 666, 746, 765, 915, 1154
Tru9I TTAA 5 cut(s) 666, 746, 765, 915, 1154
TscAI CASTG 2 cut(s) 518, 1236
TseFI GTSAC 1 cut(s) 159
TseI GCWGC 1 cut(s) 504
Tsp45I GTSAC 1 cut(s) 159
TspDTI ATGAA 5 cut(s) 20, 260, 304, 432, 593
TspRI CASTG 2 cut(s) 518, 1236
Van91I CCANNNNNTGG 1 cut(s) 113
VneI GTGCAC 1 cut(s) 1097
XapI RAATTY 2 cut(s) 605, 1135
XmnI GAANNNNTTC 1 cut(s) 839
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.