MD03G1272800.v1.1

Autophagy-related protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0006636
Physical Location & Seq
Forward (+)
35615989 .. 35621627
5639 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1272800.v1.1.491

Sequence Viewer

Length: 2982 bp
ATGAAGAAGAGTAAGGGGAAGAACAATGGCTTGTTGCCGAACTCGCTTAGGATTATTTCGTCGTGCCTTAAAACTGTGTCGACGAATGCGAGCACCGTCGCTTCTACTGTTCGCTCTGCCGGTGCGTCTGTAGCTGCTTCGATTTCTGCTTCCGAGGATCAGAAGGATCAGGTAACGTGGGCTGGCTTTGACAGGCTAGAGCTTAGTCACTCTACCTTCAAGCATGTTCTCTTACTCGGTTATCAGAATGGGTTTCAAGTTTTCGATATGGAAGATGCCTCAAATTTCAGTGAACTAGTTTCAAAGCGCGATGGCCCGGTTTCATTCTTACAGATGCAGCCCTGTCCTGCTGCATCTGATGGTAACCAAGGGTTCATAACTGCGCATCCTTTGCTGTTGGTTGTTGCTGGAGATGACACTAATGGTGTGGGAATTGTTCAGAATACTGGCCACATGGGAGGGACAGGCAGAGAGGGTAATTTGGAGTCTCGGCCTGGTAACCCTGTCAGCTCTCCTACAGCTGTTCGGTTTTACTCCCTTAGGTCCCAAGGTTACGTACATGTTCTGAGGTTTCGGTCTGCTGTGTGTATGATTAGATGCAGTCCTCGGATAGTAGCTGTGGGTCTTGCAAGTCAAATTTACTGCTTTGATGCGCTCACCCTCGAAAACAGATTCAGTGTTCTTACCTATCCTGTTCCTCAGCTAGCAGGGCAAGGATCAAATGGATTTAATGTTGGTTACGGCCCAATGACTGTGGGTCCTAGGTGGTTAGCCTATGCTTCCAACAGCCCTCTAGTGTCAAACGCAAGTTGCTTAGGCCCCCAAAACCTCACTCCTTCTCCCGGAGTTAGTCCATCTACATCACCTGGCAATGGTAGTTATGTGGCTCGTTATGCAGTGGAGTCTAGTAAACAGTTGGCTGCTGGAATAATTAACCTGGGTGACATGGGATGCAAAACCTTGTATAAATACTGTCAAGAGATGCTCCCTGATGGATCTAACTCTCCAATATCGTCAAATTCAGGCTGGAAAGTTAGCAGGCATGCAGGCACAGAAATGGATAATGCAGGGATGGTTGTTGTCAAAGATTTTATTACCCGAGCTGTTATATCGCAGTTTAAAGCTCATACCAGTCCAATATCTGCACTGTGTTTCGATCCTAGTGGGACCCTTCTCGTTACTGCCTCAGTATATGGAAATAATATTAATATTTTTCGGATAATGCCTTCATGCAAACGCAGTGGATCAGGTGTTCAAAACTTTGATTGGAGCTCTTCTCATGTGCATCTTTACAAACTGCATCGTGGAATATTATCAGCTATGATCCAGGACATTTGCTTTAGTCATTACAGTCAGTGGGTTGCTATTGTTTCGTCCAAGGGGACCTGCCATGTTTTTGTTTTATCCCCTTTTGGTGGCGATGCTGGATTTCAGCTTCTTAATAATCACGGTGAAGAACCCTCCCTGTGCCCAGTTTTAACCCTACCTTGGTGGTCTGCTTCTTCTTGCATCATTAATCAACAATCTTTTCCACCGCCACCACCTGTTGCTCTTTCCGTGGTGAGCAGGATAAAGTACAGTAGTTTTGGATGGCTTAGCACAGTCAACAATGCCACTGCTTCAGCAACAGGGAAGGTCTTTGTGCCATCTGGTGCTGTTGCTGCAGTTTTTCATAATTCTTTATGTCAGAGTCTTCAGCACTGTAACTCAAGGAAGAGTACCTTGGAACATCTCTTAGTTTATACTCCATCGGGTCATGTAGTTCAACATGAACTTCAGCCAAGGACAGGGGTAGAACAGAGTCATAGTGGTCTAAGTACCCAGGTCGCCACGTCTATGCATATGCAGGACGAAGAGCTGAGAGTGAAAGTTGAACCTATTCAATGGTGGGACGTGTGTAGGCGGTCAGACTGGCCAGAAAGGGAGGACGGTAGTTTTGGTACCACATCCGATAGACAAGAGGTGGCTGACATAATCCAGAACAAATCAGGTTCTGATGGTACTCATAGAATGGAGTCTCTAGACTTAAATGGTGCTGTTGGGGGCGAAAGGAGACTTAAAACTTATTCTGGGAAGGTCCATGATAGGTCCCACTGGTACCTCTCTAATGCAGAGGTGCAAATCAGTTCTTTGAGATTACCAATATGGCAAAAGTCTAAGATTTGTTTTTACATGATGGGCTGTCCAAGAGCTGATAGTTTCATCGGTGGAGAGTTCGAAACTGAAAAGGTCCCTGTTCATGAAATTGAAATGAGACAGAAGGATTTGTTGCCTGTTTTTGAGCAGTTCCATTTCATCAAATCAAGCTGGGATGACAGAAGCCTTGCTGGTGGGAGGTATCCCAGTCATACTTCGCCAGCTCATCAAGCTGAAGACAAGACCTTGGAAGAGACTGTTATATGTCACTCGAACCCTGCATCACTTAGCTCCACTGAAAGCTCAGATGGGGGTTCGTCAAGAAGAATTGAGCATTTTCTCGACTTGGATCAAATGAACAATGAGAAGCCCCGCACAACAGTGTATCAGACCTTGGATGGCTCGGAAAGAAGAGCCAATACAATTGTTGAGCCTTCGTTAGAAAACCATGTATCTTTCAGCATTCGGGGTACTCCATCCGAACATTTTAAGAACATCAATTCTCAAGTCAACAGCTGTGTCACCAACAGTTTGCCTGTGTTAGAAAGTAAATTGAGTTCAGGAGGAAGAGTTTCTGTAGAAGAAGTCCCAGCATTGAAAGCAATTGGGGTCAGGGAGGTCTCGGTTTTATACTCTGATCAACATGCTTCGAGTACCGATATTGTGGCAGAGGGGGCTTCCACTTTGCAGCATCCGATAGATCTTTCACAGTTTTTCCAGGAGGAGCATTGTAATGCACAGGAGAAGAATGGATGCAATGGCCTAACTGATGTCGTAGGTGACGATGTTGACAGCGACAGTAGCCACTGCGATAAGATGAAACCCGAAGACAAGGAAGATGGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000226 GO:0000785 GO:0001952 GO:0001953 GO:0003674 GO:0003682 GO:0005102 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005694 GO:0005730 GO:0005737 GO:0005856 GO:0005874 GO:0005881 GO:0006355 GO:0006357 GO:0006810 GO:0006950 GO:0006996 GO:0007010 GO:0007017 GO:0007030 GO:0007162 GO:0007275 GO:0008047 GO:0008092 GO:0008134 GO:0008150 GO:0009605 GO:0009653 GO:0009889 GO:0009891 GO:0009893 GO:0009987 GO:0009991 GO:0010256 GO:0010468 GO:0010556 GO:0010557 GO:0010594 GO:0010595 GO:0010604 GO:0010628 GO:0010632 GO:0010634 GO:0010638 GO:0010698 GO:0010810 GO:0010812 GO:0015630 GO:0015631 GO:0016043 GO:0016192 GO:0019219 GO:0019222 GO:0019899 GO:0030155 GO:0030234 GO:0030334 GO:0030335 GO:0031023 GO:0031252 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031344 GO:0031346 GO:0031667 GO:0031974 GO:0031981 GO:0032386 GO:0032388 GO:0032501 GO:0032502 GO:0032878 GO:0032879 GO:0032880 GO:0032956 GO:0032970 GO:0033043 GO:0033157 GO:0034260 GO:0035035 GO:0035148 GO:0035239 GO:0035257 GO:0035295 GO:0035327 GO:0040012 GO:0040017 GO:0042221 GO:0042393 GO:0042594 GO:0043085 GO:0043086 GO:0043087 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043547 GO:0043627 GO:0044087 GO:0044089 GO:0044092 GO:0044093 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044430 GO:0044444 GO:0044446 GO:0044464 GO:0045111 GO:0045893 GO:0045935 GO:0045944 GO:0048487 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048646 GO:0048856 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051049 GO:0051050 GO:0051128 GO:0051129 GO:0051130 GO:0051171 GO:0051173 GO:0051179 GO:0051222 GO:0051223 GO:0051234 GO:0051239 GO:0051240 GO:0051252 GO:0051254 GO:0051270 GO:0051272 GO:0051336 GO:0051345 GO:0051346 GO:0051427 GO:0051489 GO:0051491 GO:0051493 GO:0051495 GO:0051716 GO:0051893 GO:0051895 GO:0060255 GO:0060341 GO:0060491 GO:0065007 GO:0065009 GO:0070013 GO:0070201 GO:0070887 GO:0071391 GO:0071840 GO:0080090 GO:0090087 GO:0090109 GO:0090316 GO:0090630 GO:0098772 GO:0099080 GO:0099081 GO:0099512 GO:0099513 GO:0120032 GO:0120034 GO:0120035 GO:1901888 GO:1901889 GO:1902680 GO:1903391 GO:1903392 GO:1903506 GO:1903508 GO:1903827 GO:1903829 GO:1904951 GO:2000112 GO:2000114 GO:2000145 GO:2000147 GO:2000249 GO:2000251 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
BCAS3 WD40

Protein Analysis

994

Amino Acids

107.98

Weight (kDa)

6.21

Isoelectric Point (pI)

46.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
BCAS3_WD40 PF21034 173 - 475 5e-29 BCAS3, WD40 repeat
BCAS3 PF12490 614 - 761 3.5e-45 BCAS3 microtubule associated cell migration factor, C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 384
Acc36I ACCTGC 1 cut(s) 1394
Acc65I GGTACC 2 cut(s) 1940, 2097
AccB1I GGYRCC 2 cut(s) 1940, 2097
AccI GTMKAC 1 cut(s) 80
AccII CGCG 1 cut(s) 309
AciI CCGC 3 cut(s) 1535, 1903, 2509
AclWI GGATC 8 cut(s) 165, 174, 724, 1003, 1151, 1252, 1318, 2493
AcoI YGGCCR 2 cut(s) 448, 1913
AcsI RAATTY 3 cut(s) 283, 636, 1018
AcuI CTGAAG 4 cut(s) 1605, 1679, 1760, 2391
AfaI GTAC 9 cut(s) 558, 1577, 1720, 1819, 1942, 2002, 2099, 2608, 2791
AfiI CCNNNNNNNGG 5 cut(s) 842, 872, 1415, 1488, 1787
AflIII ACRYGT 2 cut(s) 559, 1893
AgsI TTSAA 9 cut(s) 220, 257, 303, 1256, 1766, 1874, 1883, 2249, 2734
AhdI GACNNNNNGTC 1 cut(s) 756
AhlI ACTAGT 1 cut(s) 295
AjiI CACGTC 2 cut(s) 1833, 1894
AjnI CCWGG 6 cut(s) 493, 865, 936, 1326, 1821, 2853
AjuI GAANNNNNNNTTGG 2 cut(s) 1706, 1738
AleI CACNNNNGTG 1 cut(s) 2516
AloI GAACNNNNNNTCC 2 cut(s) 1236, 1268
Alw21I GWGCWC 2 cut(s) 95, 1274
Alw26I GTCTC 6 cut(s) 492, 2022, 2047, 2248, 2384, 2761
AlwI GGATC 8 cut(s) 165, 174, 724, 1003, 1151, 1252, 1318, 2493
AlwNI CAGNNNCTG 1 cut(s) 1994
Ama87I CYCGRG 1 cut(s) 1098
AoxI GGCC 7 cut(s) 313, 448, 491, 742, 817, 1913, 2896
ApeKI GCWGC 6 cut(s) 134, 337, 350, 920, 1661, 2824
ApoI RAATTY 3 cut(s) 283, 636, 1018
ArsI GACNNNNNNTTYG 2 cut(s) 1919, 1951
AseI ATTAAT 2 cut(s) 1206, 1515
Asp700I GAANNNNTTC 2 cut(s) 1878, 2707
Asp718I GGTACC 2 cut(s) 1940, 2097
AspA2I CCTAGG 1 cut(s) 761
AspLEI GCGC 3 cut(s) 309, 385, 655
AsuC2I CCSGG 2 cut(s) 317, 843
AsuHPI GGTGA 7 cut(s) 649, 855, 953, 1463, 1573, 2650, 2927
AsuII TTCGAA 1 cut(s) 2217
AsuNHI GCTAGC 1 cut(s) 703
AvaI CYCGRG 1 cut(s) 1098
AvaII GGWCC 7 cut(s) 543, 758, 1167, 1383, 2077, 2088, 2230
AvrII CCTAGG 1 cut(s) 761
AxyI CCTNAGG 1 cut(s) 539
BaeGI GKGCMC 1 cut(s) 1472
BalI TGGCCA 2 cut(s) 450, 1915
BanI GGYRCC 2 cut(s) 1940, 2097
BanII GRGCYC 1 cut(s) 1274
BarI GAAGNNNNNNTAC 2 cut(s) 2539, 2571
BbsI GAAGAC 3 cut(s) 1685, 2379, 2970
Bbv12I GWGCWC 2 cut(s) 95, 1274
BbvCI CCTCAGC 1 cut(s) 699
BbvI GCAGC 6 cut(s) 121, 337, 349, 907, 1648, 2836
BceAI ACGGC 1 cut(s) 757
BciT130I CCWGG 6 cut(s) 495, 867, 938, 1328, 1823, 2855
BciVI GTATCC 1 cut(s) 2349
BclI TGATCA 1 cut(s) 2773
BcnI CCSGG 2 cut(s) 317, 843
BcoDI GTCTC 6 cut(s) 492, 2022, 2047, 2248, 2384, 2761
BcuI ACTAGT 1 cut(s) 295
BfaI CTAG 8 cut(s) 197, 296, 704, 762, 794, 906, 1161, 2021
BfmI CTRYAG 4 cut(s) 129, 516, 1662, 2712
BfuAI ACCTGC 1 cut(s) 1394
BfuI GTATCC 1 cut(s) 2349
BglII AGATCT 1 cut(s) 2836
BisI GCNGC 6 cut(s) 135, 338, 351, 921, 1662, 2825
BlnI CCTAGG 1 cut(s) 761
BlpI GCTNAGC 1 cut(s) 1595
BlsI GCNGC 6 cut(s) 136, 339, 352, 922, 1663, 2826
Bme1390I CCNGG 8 cut(s) 317, 495, 843, 867, 938, 1328, 1823, 2855
Bme18I GGWCC 7 cut(s) 543, 758, 1167, 1383, 2077, 2088, 2230
BmeRI GACNNNNNGTC 1 cut(s) 756
BmeT110I CYCGRG 1 cut(s) 1098
BmgBI CACGTC 2 cut(s) 1833, 1894
BmrFI CCNGG 8 cut(s) 317, 495, 843, 867, 938, 1328, 1823, 2855
BmrI ACTGGG 2 cut(s) 1466, 2337
BmtI GCTAGC 1 cut(s) 707
BmuI ACTGGG 2 cut(s) 1466, 2337
BpiI GAAGAC 3 cut(s) 1685, 2379, 2970
BplI GAGNNNNNCTC 2 cut(s) 1261, 1293
BpmI CTGGAG 1 cut(s) 429
Bpu10I CCTNAGC 3 cut(s) 47, 699, 814
Bpu1102I GCTNAGC 1 cut(s) 1595
Bpu14I TTCGAA 1 cut(s) 2217
BpuEI CTTGAG 2 cut(s) 1693, 2625
BpuMI CCSGG 2 cut(s) 317, 843
BsaAI YACGTR 1 cut(s) 556
BsaI GGTCTC 1 cut(s) 2761
BsaXI ACNNNNNCTCC 4 cut(s) 823, 853, 2691, 2721
Bsc4I CCNNNNNNNGG 5 cut(s) 842, 872, 1415, 1488, 1787
Bse118I RCCGGY 1 cut(s) 119
Bse1I ACTGG 6 cut(s) 451, 1131, 1472, 1916, 2099, 2343
Bse21I CCTNAGG 1 cut(s) 539
Bse3DI GCAATG 2 cut(s) 877, 2899
BseBI CCWGG 6 cut(s) 495, 867, 938, 1328, 1823, 2855
BseLI CCNNNNNNNGG 5 cut(s) 842, 872, 1415, 1488, 1787
BseMI GCAATG 2 cut(s) 877, 2899
BseMII CTCAG 5 cut(s) 557, 713, 1200, 1850, 2454
BseNI ACTGG 6 cut(s) 451, 1131, 1472, 1916, 2099, 2343
BseRI GAGGAG 1 cut(s) 2873
BseSI GKGCMC 1 cut(s) 1472
BseXI GCAGC 6 cut(s) 121, 337, 349, 907, 1648, 2836
BseYI CCCAGC 2 cut(s) 2307, 2725
BsgI GTGCAG 1 cut(s) 1128
Bsh1236I CGCG 1 cut(s) 309
BshFI GGCC 7 cut(s) 315, 450, 493, 744, 819, 1915, 2898
BshNI GGYRCC 2 cut(s) 1940, 2097
BsiHKAI GWGCWC 2 cut(s) 95, 1274
BsiHKCI CYCGRG 1 cut(s) 1098
BsiSI CCGG 3 cut(s) 120, 317, 843
BslFI GGGAC 8 cut(s) 475, 529, 1180, 1396, 1904, 2074, 2216, 2708
BslI CCNNNNNNNGG 5 cut(s) 842, 872, 1415, 1488, 1787
BsmAI GTCTC 6 cut(s) 492, 2022, 2047, 2248, 2384, 2761
BsmFI GGGAC 8 cut(s) 475, 529, 1180, 1396, 1904, 2074, 2216, 2708
BsmI GAATGC 2 cut(s) 91, 2598
BsnI GGCC 7 cut(s) 315, 450, 493, 744, 819, 1915, 2898
Bso31I GGTCTC 1 cut(s) 2761
BsoBI CYCGRG 1 cut(s) 1098
Bsp119I TTCGAA 1 cut(s) 2217
Bsp1286I GDGCHC 3 cut(s) 95, 1274, 1472
Bsp1720I GCTNAGC 1 cut(s) 1595
BspACI CCGC 3 cut(s) 1535, 1903, 2509
BspANI GGCC 7 cut(s) 315, 450, 493, 744, 819, 1915, 2898
BspCNI CTCAG 5 cut(s) 558, 712, 1199, 1851, 2453
BspFNI CGCG 1 cut(s) 309
BspHI TCATGA 1 cut(s) 2239
BspMAI CTGCAG 1 cut(s) 1666
BspMI ACCTGC 1 cut(s) 1394
BspOI GCTAGC 1 cut(s) 707
BspPI GGATC 8 cut(s) 165, 174, 724, 1003, 1151, 1252, 1318, 2493
BspQI GCTCTTC 3 cut(s) 1279, 1848, 2542
BspT104I TTCGAA 1 cut(s) 2217
BspT107I GGYRCC 2 cut(s) 1940, 2097
BspTNI GGTCTC 1 cut(s) 2761
BsrDI GCAATG 2 cut(s) 877, 2899
BsrFI RCCGGY 1 cut(s) 119
BsrI ACTGG 6 cut(s) 451, 1131, 1472, 1916, 2099, 2343
BssAI RCCGGY 1 cut(s) 119
BssT1I CCWWGG 9 cut(s) 367, 547, 761, 1377, 1487, 1722, 1781, 2382, 2529
Bst2UI CCWGG 6 cut(s) 495, 867, 938, 1328, 1823, 2855
Bst6I CTCTTC 7 cut(s) 2, 1279, 1709, 1848, 2382, 2542, 2698
BstAPI GCANNNNNTGC 1 cut(s) 391
BstBAI YACGTR 1 cut(s) 556
BstBI TTCGAA 1 cut(s) 2217
BstC8I GCNNGC 7 cut(s) 91, 184, 705, 1040, 1044, 1048, 2358
BstDSI CCRYGG 1 cut(s) 1557
BstEII GGTNACC 2 cut(s) 362, 497
BstFNI CGCG 1 cut(s) 309
BstHHI GCGC 3 cut(s) 309, 385, 655
BstMAI GTCTC 6 cut(s) 492, 2022, 2047, 2248, 2384, 2761
BstNI CCWGG 6 cut(s) 495, 867, 938, 1328, 1823, 2855
BstNSI RCATGY 4 cut(s) 227, 563, 1046, 2783
BstPI GGTNACC 2 cut(s) 362, 497
BstSCI CCNGG 8 cut(s) 315, 493, 841, 865, 936, 1326, 1821, 2853
BstSFI CTRYAG 4 cut(s) 129, 516, 1662, 2712
BstSLI GKGCMC 1 cut(s) 1472
BstSNI TACGTA 1 cut(s) 556
BstUI CGCG 1 cut(s) 309
BstV1I GCAGC 6 cut(s) 121, 337, 349, 907, 1648, 2836
BstV2I GAAGAC 3 cut(s) 1685, 2379, 2970
BstX2I RGATCY 2 cut(s) 995, 2836
BstYI RGATCY 2 cut(s) 995, 2836
Bsu36I CCTNAGG 1 cut(s) 539
BsuI GTATCC 1 cut(s) 2349
BsuRI GGCC 7 cut(s) 315, 450, 493, 744, 819, 1915, 2898
BtgI CCRYGG 1 cut(s) 1557
BtgZI GCGATG 2 cut(s) 324, 1434
BtrI CACGTC 2 cut(s) 1833, 1894
BtsI GCAGTG 4 cut(s) 903, 1246, 1614, 2941
BveI ACCTGC 1 cut(s) 1394
Cac8I GCNNGC 7 cut(s) 91, 184, 705, 1040, 1044, 1048, 2358
CaiI CAGNNNCTG 1 cut(s) 1994
CciI TCATGA 1 cut(s) 2239
CfoI GCGC 3 cut(s) 309, 385, 655
Cfr10I RCCGGY 1 cut(s) 119
CseI GACGC 1 cut(s) 114
Csp6I GTAC 9 cut(s) 557, 1576, 1719, 1818, 1941, 2001, 2098, 2607, 2790
CspCI CAANNNNNGTGG 4 cut(s) 735, 770, 1222, 1257
CviQI GTAC 9 cut(s) 557, 1576, 1719, 1818, 1941, 2001, 2098, 2607, 2790
DraI TTTAAA 1 cut(s) 1120
DriI GACNNNNNGTC 1 cut(s) 756
EaeI YGGCCR 2 cut(s) 448, 1913
Eam1104I CTCTTC 7 cut(s) 2, 1279, 1709, 1848, 2382, 2542, 2698
Eam1105I GACNNNNNGTC 1 cut(s) 756
EarI CTCTTC 7 cut(s) 2, 1279, 1709, 1848, 2382, 2542, 2698
Ecl136II GAGCTC 1 cut(s) 1272
Eco105I TACGTA 1 cut(s) 556
Eco130I CCWWGG 9 cut(s) 367, 547, 761, 1377, 1487, 1722, 1781, 2382, 2529
Eco24I GRGCYC 1 cut(s) 1274
Eco31I GGTCTC 1 cut(s) 2761
Eco47I GGWCC 7 cut(s) 543, 758, 1167, 1383, 2077, 2088, 2230
Eco53kI GAGCTC 1 cut(s) 1272
Eco57I CTGAAG 4 cut(s) 1605, 1679, 1760, 2391
Eco81I CCTNAGG 1 cut(s) 539
Eco88I CYCGRG 1 cut(s) 1098
Eco91I GGTNACC 2 cut(s) 362, 497
EcoICRI GAGCTC 1 cut(s) 1272
EcoO109I RGGNCCY 7 cut(s) 543, 758, 818, 1167, 1383, 2088, 2230
EcoO65I GGTNACC 2 cut(s) 362, 497
EcoRII CCWGG 6 cut(s) 493, 865, 936, 1326, 1821, 2853
EcoT14I CCWWGG 9 cut(s) 367, 547, 761, 1377, 1487, 1722, 1781, 2382, 2529
EcoT22I ATGCAT 1 cut(s) 1842
EcoT38I GRGCYC 1 cut(s) 1274
ErhI CCWWGG 9 cut(s) 367, 547, 761, 1377, 1487, 1722, 1781, 2382, 2529
FalI AAGNNNNNCTT 2 cut(s) 1706, 1738
FaqI GGGAC 8 cut(s) 475, 529, 1180, 1396, 1904, 2074, 2216, 2708
FauI CCCGC 1 cut(s) 2516
FauNDI CATATG 1 cut(s) 1842
FbaI TGATCA 1 cut(s) 2773
FblI GTMKAC 1 cut(s) 80
Fnu4HI GCNGC 6 cut(s) 135, 338, 351, 921, 1662, 2825
FriOI GRGCYC 1 cut(s) 1274
Fsp4HI GCNGC 6 cut(s) 135, 338, 351, 921, 1662, 2825
FspBI CTAG 8 cut(s) 197, 296, 704, 762, 794, 906, 1161, 2021
FspI TGCGCA 1 cut(s) 384
GlaI GCGC 3 cut(s) 308, 384, 654
GluI GCNGC 6 cut(s) 135, 338, 351, 921, 1662, 2825
GsaI CCCAGC 2 cut(s) 2311, 2729
GsuI CTGGAG 1 cut(s) 429
HaeIII GGCC 7 cut(s) 315, 450, 493, 744, 819, 1915, 2898
HapII CCGG 3 cut(s) 120, 317, 843
HgaI GACGC 1 cut(s) 114
HhaI GCGC 3 cut(s) 309, 385, 655
Hin6I GCGC 3 cut(s) 307, 383, 653
HinP1I GCGC 3 cut(s) 307, 383, 653
HincII GTYRAC 4 cut(s) 81, 1606, 2647, 2926
HindII GTYRAC 4 cut(s) 81, 1606, 2647, 2926
HinfI GANTC 6 cut(s) 485, 672, 902, 1690, 1801, 2015
HpaII CCGG 3 cut(s) 120, 317, 843
HphI GGTGA 7 cut(s) 649, 855, 953, 1463, 1573, 2650, 2927
Hpy166II GTNNAC 6 cut(s) 81, 293, 911, 1606, 2647, 2926
Hpy188III TCNNGA 7 cut(s) 977, 1978, 2021, 2240, 2457, 2477, 2697
Hpy8I GTNNAC 6 cut(s) 81, 293, 911, 1606, 2647, 2926
Hpy99I CGWCG 3 cut(s) 64, 85, 101
HpyAV CCTTC 9 cut(s) 157, 226, 846, 1181, 1236, 1627, 2068, 2254, 2580
HpyCH4IV ACGT 4 cut(s) 176, 555, 1832, 1893
HpySE526I ACGT 4 cut(s) 176, 555, 1832, 1893
HspAI GCGC 3 cut(s) 307, 383, 653
KflI GGGWCCC 1 cut(s) 1167
KpnI GGTACC 2 cut(s) 1944, 2101
Ksp22I TGATCA 1 cut(s) 2773
LguI GCTCTTC 3 cut(s) 1279, 1848, 2542
LmnI GCTCC 4 cut(s) 990, 1269, 2432, 2860
Lsp1109I GCAGC 6 cut(s) 121, 337, 349, 907, 1648, 2836
MaeI CTAG 8 cut(s) 197, 296, 704, 762, 794, 906, 1161, 2021
MaeII ACGT 4 cut(s) 176, 555, 1832, 1893
MfeI CAATTG 2 cut(s) 2559, 2739
MflI RGATCY 2 cut(s) 995, 2836
MhlI GDGCHC 3 cut(s) 95, 1274, 1472
MlsI TGGCCA 2 cut(s) 450, 1915
MluNI TGGCCA 2 cut(s) 450, 1915
MlyI GAGTC 5 cut(s) 494, 911, 1699, 1810, 2024
MmeI TCCRAC 1 cut(s) 807
Mox20I TGGCCA 2 cut(s) 450, 1915
Mph1103I ATGCAT 1 cut(s) 1842
MroXI GAANNNNTTC 2 cut(s) 1878, 2707
MscI TGGCCA 2 cut(s) 450, 1915
MslI CAYNNNNRTG 4 cut(s) 1055, 1835, 2516, 2868
Msp20I TGGCCA 2 cut(s) 450, 1915
MspA1I CMGCKG 2 cut(s) 521, 2652
MspI CCGG 3 cut(s) 120, 317, 843
MspR9I CCNGG 8 cut(s) 317, 495, 843, 867, 938, 1328, 1823, 2855
MunI CAATTG 2 cut(s) 2559, 2739
Mva1269I GAATGC 2 cut(s) 91, 2598
MvaI CCWGG 6 cut(s) 495, 867, 938, 1328, 1823, 2855
MvnI CGCG 1 cut(s) 309
NciI CCSGG 2 cut(s) 317, 843
NdeI CATATG 1 cut(s) 1842
NheI GCTAGC 1 cut(s) 703
NmeAIII GCCGAG 1 cut(s) 469
NmuCI GTSAC 5 cut(s) 206, 941, 2402, 2656, 2915
NsbI TGCGCA 1 cut(s) 384
NsiI ATGCAT 1 cut(s) 1842
NspI RCATGY 4 cut(s) 227, 563, 1046, 2783
NspV TTCGAA 1 cut(s) 2217
OliI CACNNNNGTG 1 cut(s) 2516
PaeI GCATGC 1 cut(s) 1046
PagI TCATGA 1 cut(s) 2239
PciI ACATGT 1 cut(s) 559
PciSI GCTCTTC 3 cut(s) 1279, 1848, 2542
PcsI WCGNNNNNNNCGW 2 cut(s) 86, 2916
PctI GAATGC 2 cut(s) 91, 2598
PdmI GAANNNNTTC 2 cut(s) 1878, 2707
PfeI GAWTC 1 cut(s) 672
PfoI TCCNGGA 3 cut(s) 841, 1326, 2853
PkrI GCNGC 6 cut(s) 136, 339, 352, 922, 1663, 2826
PleI GAGTC 5 cut(s) 493, 910, 1698, 1809, 2023
PpsI GAGTC 5 cut(s) 493, 910, 1698, 1809, 2023
Ppu21I YACGTR 1 cut(s) 556
PpuMI RGGWCCY 6 cut(s) 543, 758, 1167, 1383, 2088, 2230
PscI ACATGT 1 cut(s) 559
PshBI ATTAAT 2 cut(s) 1206, 1515
Psp124BI GAGCTC 1 cut(s) 1274
Psp5II RGGWCCY 6 cut(s) 543, 758, 1167, 1383, 2088, 2230
Psp6I CCWGG 6 cut(s) 493, 865, 936, 1326, 1821, 2853
PspEI GGTNACC 2 cut(s) 362, 497
PspFI CCCAGC 2 cut(s) 2307, 2725
PspGI CCWGG 6 cut(s) 493, 865, 936, 1326, 1821, 2853
PspPPI RGGWCCY 6 cut(s) 543, 758, 1167, 1383, 2088, 2230
PsrI GAACNNNNNNTAC 2 cut(s) 2677, 2709
PstI CTGCAG 1 cut(s) 1666
PstNI CAGNNNCTG 1 cut(s) 1994
PsuI RGATCY 2 cut(s) 995, 2836
PvuII CAGCTG 2 cut(s) 521, 2652
RsaI GTAC 9 cut(s) 558, 1577, 1720, 1819, 1942, 2002, 2099, 2608, 2791
RsaNI GTAC 9 cut(s) 557, 1576, 1719, 1818, 1941, 2001, 2098, 2607, 2790
RseI CAYNNNNRTG 4 cut(s) 1055, 1835, 2516, 2868
SacI GAGCTC 1 cut(s) 1274
SalI GTCGAC 1 cut(s) 79
SapI GCTCTTC 3 cut(s) 1279, 1848, 2542
SatI GCNGC 6 cut(s) 135, 338, 351, 921, 1662, 2825
SchI GAGTC 5 cut(s) 494, 911, 1699, 1810, 2024
ScrFI CCNGG 8 cut(s) 317, 495, 843, 867, 938, 1328, 1823, 2855
SduI GDGCHC 3 cut(s) 95, 1274, 1472
SfcI CTRYAG 4 cut(s) 129, 516, 1662, 2712
SfuI TTCGAA 1 cut(s) 2217
SinI GGWCC 7 cut(s) 543, 758, 1167, 1383, 2077, 2088, 2230
SmiMI CAYNNNNRTG 4 cut(s) 1055, 1835, 2516, 2868
SmlI CTYRAG 2 cut(s) 1708, 2640
SmoI CTYRAG 2 cut(s) 1708, 2640
SnaBI TACGTA 1 cut(s) 556
SpeI ACTAGT 1 cut(s) 295
SphI GCATGC 1 cut(s) 1046
SsiI CCGC 3 cut(s) 1535, 1903, 2509
SspI AATATT 3 cut(s) 1204, 1210, 1311
SspMI CTAG 8 cut(s) 197, 296, 704, 762, 794, 906, 1161, 2021
SstI GAGCTC 1 cut(s) 1274
StyD4I CCNGG 8 cut(s) 315, 493, 841, 865, 936, 1326, 1821, 2853
StyI CCWWGG 9 cut(s) 367, 547, 761, 1377, 1487, 1722, 1781, 2382, 2529
TaiI ACGT 4 cut(s) 179, 558, 1835, 1896
TaqI TCGA 9 cut(s) 80, 140, 264, 663, 1155, 2217, 2408, 2478, 2786
TaqII GACCGA 1 cut(s) 564
TatI WGTACW 1 cut(s) 1575
TfiI GAWTC 1 cut(s) 672
TseFI GTSAC 5 cut(s) 206, 941, 2402, 2656, 2915
TseI GCWGC 6 cut(s) 134, 337, 350, 920, 1661, 2824
Tsp45I GTSAC 5 cut(s) 206, 941, 2402, 2656, 2915
TspGWI ACGGA 1 cut(s) 1546
VpaK11BI GGWCC 7 cut(s) 543, 758, 1167, 1383, 2077, 2088, 2230
VspI ATTAAT 2 cut(s) 1206, 1515
XapI RAATTY 3 cut(s) 283, 636, 1018
XbaI TCTAGA 1 cut(s) 2020
XceI RCATGY 4 cut(s) 227, 563, 1046, 2783
XmaJI CCTAGG 1 cut(s) 761
XmiI GTMKAC 1 cut(s) 80
XmnI GAANNNNTTC 2 cut(s) 1878, 2707
XspI CTAG 8 cut(s) 197, 296, 704, 762, 794, 906, 1161, 2021
Zsp2I ATGCAT 1 cut(s) 1842
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.