Rroxscaffold_7G00175380

Autophagy-related protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0006636
Physical Location & Seq
Reverse (-)
14955607 .. 14961336
5730 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00175380.1

Sequence Viewer

Length: 2991 bp
ATGAAGAAAGGGAAGGGGAGGAACAACGGCGGCTTGTTGCCGAGCTCGCTGAGGATCATATCGTCGTGCCTCAAGACTGTTTCGACGAATGCCAGCACCGTCGCTTCTACCGTTCGCTCCGCCGGTGCGTCCGTCGCTGCTTCTATTTCTGCTTCCGAGGATCAGAAGGATCAGTTTGGATTCTCTGGAATTAATTTTGGTGTTAGGCTGAAGGTAACCTGGGCTGGCTTTGACAGGCTAGAGCTTGGTCATTCTGCCTTCAAACGCGTTCTGTTGCTTGGTTATCAGAATGGATTTCAAGTTTTTGATGTCGAGGATGCCTCCAACTACAGCGAACTGGTTTCAAAGCGTGATGGCCCAGTTTCGTTCTTACAGATGCAGCCCTATCCTGCAGCATCTGATGATGAGGAGGGATTTAGAGCTTCTCATCCTATGTTGCTGGTTGTTGCTGGAGATGACACCAATGGTTCAGGCGTTGTTCAAAATCACAGCAACTTGGGAGGCTTAGGAAGAGATGGTCATTTGGAGTCTCGGCCAGGAAACCCTTTCAGCTCTCCTACAGCTGTTCGGTTTTACTCGCTCAGGTCCCACAGTTATGTGCATGTTCTGAGATTTCGGTCTGCTGTCTGTATGATTAGATGCAGTCCTCGGATAGTAGCTGTTGGTCTTGCAAGTCAAATATATTGCTTTGATGCGCTCACCCTCGAAAATAAATTTAGTGTTCTTACCTATCCTGTTCCTCAGCTAGCAGGACAAGGATCCTCTGGGTTTAACGTAGGTTATGGCCCAATGGCTGTGGGTCCTAGGTGGCTTGCTTATGCTTCCAACAGCCCACTCATGTCAAACACAAGTCGCTTAGGTCCCCACAATCTTACTCCTTCTCCAGGAGTTAGTCCATCTACATCACCTGGTAATGGTAGTTACGTGGCTCGTTATGCAATGGAGTCTAGTAAACAGCTGGCCGCTGGAATTATCAACCTGGGTGACATGGGATGCAAAACCTTGTACAAATACTGTCAAGAGCTCCTCCCAGATGGGTCTAGTTCTCCAGTATCATCAAATTCAGGCTGGAAAGTTAGCAGGCTTGCCGGAACAGAAATGGATAGTGCTGGGATGGTTGTTGTCAAAGATTTTGTTACCCGAGCTGTTATATCGCAGTTTAAAGCTCATACTAGTCCAATATCTGCACTGTGTTTTGATCCCAGTGGGACCCTTCTTGTTACTGCCTCAATATATGGAAATAACATTAATATTTTTCGGATAATGCCTTCATGCAAACGCAATGGATCAAGCGTTCAGAACATGAATTGGAGCTCGTCTCATGTGCATCTATACAAACTGCATCGTGGAATAACATCAGCTATGATCCAGGACATTTGCTTCAGTCATTATTCTCAATGGGTTGCAATTGTTTCATCTAAGGGGACCTGCCATGTTTTTGTTTTATCCCCTTTTGGTGGTGATGCTGGATTTCAAGTTCAACATTCCCAGGGTGAAGAACCCACTCTATATCCAGTTTTATCTCTTCCTTGGTGGTCTACTTCTTCTTGCATCATGAGCCAACAATCTTTTCCACCCCCACCGCCTGTTGTTCTTTCAGTTGTGAGCAGAATAAAGTATAGTAGTTTTGGATGGCTTAGCACAGTCAACAGTGCCGCTGGTTCCACAACAGGAAAGGTATTTGTACCATCAGGTGCTGTTGCGGCTGTTTTTCATAATTCCCTTTCTCAAAGTGTTCAGCACAGTAACTCAAGGGCTGGTACCTTGGAGCATCTATTAGTTTATACTCCATCAGGTCATGTAGTTCAACATGAACTTCAGCCAAGAGTAGGGGTAGAACAAAGTCATAGTGGTTTGAATACCCAGACAGCTACATACATGCACATGCAGGAGGAAGACCTGAGAGTGAAAGTTGAACCTATACAGTGGTGGGATGTATGCAGACGTTCAGATTGGCCAGAAAGAGAGGACTGTATTTTAGGAATTAACGCTGATCGTCAAGATGTGGCTGAAACAATTCAAAGGAAATCAGGTTCTGATGGTACTTATGCATTGGAGTTTCTTGACTTAAATGGTGGTGTTGAGGGGAAAAGGAACCTGGAAACGCATAGGTCCAGGAATATTTCTAATGCAGAGATTTGTTTTTATACAATGGAGTGTCAAGGAGTTGATAGTTTTTCTGGTGGAAAGTTTGAAGTTGAAAAGGTCCCTGTTCATGAAATTGAAATGAGACAGAAGGAATTGTTGCCTGTTTTTCACCAATTCCATAGCATCAAATCAAGCTGGAATGATAGTTACGGTTGTTCTGAGCTTCTCAGTTCTAGTCGGAAAAATTCAAATCATTCTTCGTCAGAATCTCATCAAGCTGAAGGCAAGATCTCAGAAGAGACTGTTATTTGTCACTCAAACCCTGCATCACTTAGCTCCACAGAAAGCTCAGATGGGGGTTCATCAAGAAGAATTGAGCATTCACTAGATTTCGATCAAATCAACAGTGATCTACCCCGCTCTCCCATTTTTCAGACTTTGAATTGTCCGGAAAGAACAGCAAATGCTATTCTTGCGTCTTCATTTCAAAACCATACATCTCTCGGCACTCTGTGTGCTCCATCTGAACATTTTAAGAACATTGGTTCTCAAGTCAGGAGTTGCGCTGTATTAGAAAGTAAGCTTCTCCCAGTAGGTAGATTCTGTGCTGAAGAAGGCCTGTCGGTGAAAACAATTGGGATGAGTGAGGACACAGATTTATACACGGATCAACATGCTTCAAGTACTGTTCTTGTGACAGAGGGGGATTCCAATCTGCAGCGTCCAATAGATCTTTCGCAGTTTTTCCAGGAGGGGCATTGTAAGGCATTGGAGCAGAATGGATGCCTATCCGGCGTCATAACCGATGATAGTGATGGTAGCCAATGCGATAAGGGGAAACCTGACGATGAAGAAAATAGCGAAATGCTTGGAGGCATGTTCGCCTTTTCTGATGAAGGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000226 GO:0000785 GO:0001952 GO:0001953 GO:0003674 GO:0003682 GO:0005102 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005694 GO:0005730 GO:0005737 GO:0005856 GO:0005874 GO:0005881 GO:0006355 GO:0006357 GO:0006810 GO:0006950 GO:0006996 GO:0007010 GO:0007017 GO:0007030 GO:0007162 GO:0007275 GO:0008047 GO:0008092 GO:0008134 GO:0008150 GO:0009605 GO:0009653 GO:0009889 GO:0009891 GO:0009893 GO:0009987 GO:0009991 GO:0010256 GO:0010468 GO:0010556 GO:0010557 GO:0010594 GO:0010595 GO:0010604 GO:0010628 GO:0010632 GO:0010634 GO:0010638 GO:0010698 GO:0010810 GO:0010812 GO:0015630 GO:0015631 GO:0016043 GO:0016192 GO:0019219 GO:0019222 GO:0019899 GO:0030155 GO:0030234 GO:0030334 GO:0030335 GO:0031023 GO:0031252 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031344 GO:0031346 GO:0031667 GO:0031974 GO:0031981 GO:0032386 GO:0032388 GO:0032501 GO:0032502 GO:0032878 GO:0032879 GO:0032880 GO:0032956 GO:0032970 GO:0033043 GO:0033157 GO:0034260 GO:0035035 GO:0035148 GO:0035239 GO:0035257 GO:0035295 GO:0035327 GO:0040012 GO:0040017 GO:0042221 GO:0042393 GO:0042594 GO:0043085 GO:0043086 GO:0043087 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043547 GO:0043627 GO:0044087 GO:0044089 GO:0044092 GO:0044093 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044430 GO:0044444 GO:0044446 GO:0044464 GO:0045111 GO:0045893 GO:0045935 GO:0045944 GO:0048487 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048646 GO:0048856 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051049 GO:0051050 GO:0051128 GO:0051129 GO:0051130 GO:0051171 GO:0051173 GO:0051179 GO:0051222 GO:0051223 GO:0051234 GO:0051239 GO:0051240 GO:0051252 GO:0051254 GO:0051270 GO:0051272 GO:0051336 GO:0051345 GO:0051346 GO:0051427 GO:0051489 GO:0051491 GO:0051493 GO:0051495 GO:0051716 GO:0051893 GO:0051895 GO:0060255 GO:0060341 GO:0060491 GO:0065007 GO:0065009 GO:0070013 GO:0070201 GO:0070887 GO:0071391 GO:0071840 GO:0080090 GO:0090087 GO:0090109 GO:0090316 GO:0090630 GO:0098772 GO:0099080 GO:0099081 GO:0099512 GO:0099513 GO:0120032 GO:0120034 GO:0120035 GO:1901888 GO:1901889 GO:1902680 GO:1903391 GO:1903392 GO:1903506 GO:1903508 GO:1903827 GO:1903829 GO:1904951 GO:2000112 GO:2000114 GO:2000145 GO:2000147 GO:2000249 GO:2000251 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
BCAS3 WD40

Protein Analysis

996

Amino Acids

107.98

Weight (kDa)

6.04

Isoelectric Point (pI)

53.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
BCAS3_WD40 PF21034 187 - 492 9.9e-32 BCAS3, WD40 repeat
BCAS3 PF12490 627 - 753 7.2e-25 BCAS3 microtubule associated cell migration factor, C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1436
Acc65I GGTACC 1 cut(s) 1760
AccB1I GGYRCC 1 cut(s) 1760
AccBSI CCGCTC 1 cut(s) 2508
AccI GTMKAC 1 cut(s) 1538
AccII CGCG 1 cut(s) 267
AccIII TCCGGA 1 cut(s) 2536
AciI CCGC 7 cut(s) 30, 120, 963, 1583, 1656, 1703, 2506
AclWI GGATC 9 cut(s) 62, 168, 177, 753, 766, 1193, 1294, 1360, 2763
AcoI YGGCCR 3 cut(s) 533, 960, 1955
AcsI RAATTY 3 cut(s) 713, 1060, 2332
AcuI CTGAAG 5 cut(s) 230, 1366, 1802, 2388, 2718
AcyI GRCGYC 1 cut(s) 2883
AdeI CACNNNGTG 1 cut(s) 2601
AfaI GTAC 5 cut(s) 1007, 1686, 1762, 2044, 2773
AfiI CCNNNNNNNGG 4 cut(s) 884, 914, 1457, 1829
AflIII ACRYGT 1 cut(s) 265
AhlI ACTAGT 1 cut(s) 1172
AjuI GAANNNNNNNTTGG 4 cut(s) 1554, 1586, 2806, 2838
AloI GAACNNNNNNTCC 6 cut(s) 1278, 1310, 1461, 1493, 2017, 2049
Alw21I GWGCWC 4 cut(s) 47, 1026, 1316, 2608
Alw26I GTCTC 4 cut(s) 534, 1323, 2224, 2381
AlwI GGATC 9 cut(s) 62, 168, 177, 753, 766, 1193, 1294, 1360, 2763
AlwNI CAGNNNCTG 3 cut(s) 398, 1697, 2036
Ama87I CYCGRG 1 cut(s) 1140
Aor13HI TCCGGA 1 cut(s) 2536
AoxI GGCC 6 cut(s) 355, 533, 784, 960, 1955, 2704
ApeKI GCWGC 4 cut(s) 137, 379, 392, 2806
ApoI RAATTY 3 cut(s) 713, 1060, 2332
AseI ATTAAT 2 cut(s) 192, 1248
Asp700I GAANNNNTTC 1 cut(s) 2016
Asp718I GGTACC 1 cut(s) 1760
AspA2I CCTAGG 1 cut(s) 803
AspLEI GCGC 2 cut(s) 697, 2654
AspS9I GGNCC 9 cut(s) 356, 585, 785, 800, 860, 1209, 1425, 2112, 2206
AsuHPI GGTGA 7 cut(s) 691, 897, 995, 1472, 1505, 2249, 2725
AsuNHI GCTAGC 1 cut(s) 745
AvaI CYCGRG 1 cut(s) 1140
AvaII GGWCC 7 cut(s) 585, 800, 860, 1209, 1425, 2112, 2206
AvrII CCTAGG 1 cut(s) 803
BalI TGGCCA 1 cut(s) 1957
BamHI GGATCC 1 cut(s) 758
BanI GGYRCC 1 cut(s) 1760
BanII GRGCYC 3 cut(s) 47, 1026, 1316
BbsI GAAGAC 2 cut(s) 1902, 2559
Bbv12I GWGCWC 4 cut(s) 47, 1026, 1316, 2608
BbvCI CCTCAGC 2 cut(s) 50, 741
BbvI GCAGC 4 cut(s) 124, 391, 404, 2818
BceAI ACGGC 1 cut(s) 43
BcoDI GTCTC 4 cut(s) 534, 1323, 2224, 2381
BcuI ACTAGT 1 cut(s) 1172
BfaI CTAG 8 cut(s) 239, 746, 804, 948, 1041, 1173, 2322, 2474
BfmI CTRYAG 4 cut(s) 328, 390, 558, 2804
BfuAI ACCTGC 1 cut(s) 1436
BglI GCCNNNNNGGC 1 cut(s) 2880
BglII AGATCT 2 cut(s) 2376, 2818
BisI GCNGC 8 cut(s) 31, 138, 380, 393, 963, 1656, 1704, 2807
BlnI CCTAGG 1 cut(s) 803
BlpI GCTNAGC 1 cut(s) 1637
BlsI GCNGC 8 cut(s) 32, 139, 381, 394, 964, 1657, 1705, 2808
BmcAI AGTACT 1 cut(s) 2773
Bme18I GGWCC 7 cut(s) 585, 800, 860, 1209, 1425, 2112, 2206
BmeT110I CYCGRG 1 cut(s) 1140
BmgT120I GGNCC 9 cut(s) 356, 585, 785, 800, 860, 1209, 1425, 2112, 2206
BmrI ACTGGG 3 cut(s) 353, 1197, 2672
BmtI GCTAGC 1 cut(s) 749
BmuI ACTGGG 3 cut(s) 353, 1197, 2672
BpiI GAAGAC 2 cut(s) 1902, 2559
BplI GAGNNNNNCTC 4 cut(s) 305, 337, 1303, 1335
BpmI CTGGAG 3 cut(s) 471, 867, 1032
Bpu10I CCTNAGC 5 cut(s) 50, 505, 581, 741, 856
Bpu1102I GCTNAGC 1 cut(s) 1637
BpuEI CTTGAG 3 cut(s) 56, 1735, 2622
BsaAI YACGTR 1 cut(s) 925
BsaBI GATNNNNATC 3 cut(s) 2481, 2799, 2875
BsaHI GRCGYC 1 cut(s) 2883
BsaJI CCNNGG 9 cut(s) 156, 219, 647, 803, 979, 1488, 1489, 1529, 1764
BsaWI WCCGGW 1 cut(s) 2536
BsaXI ACNNNNNCTCC 2 cut(s) 865, 895
Bsc4I CCNNNNNNNGG 4 cut(s) 884, 914, 1457, 1829
Bse118I RCCGGY 1 cut(s) 122
Bse1I ACTGG 6 cut(s) 342, 359, 1049, 1203, 1514, 2678
Bse3DI GCAATG 2 cut(s) 945, 1288
Bse8I GATNNNNATC 3 cut(s) 2481, 2799, 2875
BseAI TCCGGA 1 cut(s) 2536
BseDI CCNNGG 9 cut(s) 156, 219, 647, 803, 979, 1488, 1489, 1529, 1764
BseGI GGATG 8 cut(s) 322, 427, 998, 1119, 1637, 1939, 2733, 2876
BseJI GATNNNNATC 3 cut(s) 2481, 2799, 2875
BseLI CCNNNNNNNGG 4 cut(s) 884, 914, 1457, 1829
BseMI GCAATG 2 cut(s) 945, 1288
BseMII CTCAG 9 cut(s) 41, 595, 599, 755, 1892, 2298, 2329, 2394, 2451
BseNI ACTGG 6 cut(s) 342, 359, 1049, 1203, 1514, 2678
BseRI GAGGAG 2 cut(s) 422, 1016
BseXI GCAGC 4 cut(s) 124, 391, 404, 2818
BseYI CCCAGC 1 cut(s) 1109
BsgI GTGCAG 1 cut(s) 1170
Bsh1236I CGCG 1 cut(s) 267
BshFI GGCC 6 cut(s) 357, 535, 786, 962, 1957, 2706
BshNI GGYRCC 1 cut(s) 1760
BsiHKAI GWGCWC 4 cut(s) 47, 1026, 1316, 2608
BsiHKCI CYCGRG 1 cut(s) 1140
BsiSI CCGG 4 cut(s) 123, 1089, 2537, 2880
BslFI GGGAC 5 cut(s) 571, 846, 1222, 1438, 2192
BslI CCNNNNNNNGG 4 cut(s) 884, 914, 1457, 1829
BsmAI GTCTC 4 cut(s) 534, 1323, 2224, 2381
BsmBI CGTCTC 1 cut(s) 1323
BsmFI GGGAC 5 cut(s) 571, 846, 1222, 1438, 2192
BsmI GAATGC 2 cut(s) 94, 2467
BsnI GGCC 6 cut(s) 357, 535, 786, 962, 1957, 2706
BsoBI CYCGRG 1 cut(s) 1140
Bsp1286I GDGCHC 4 cut(s) 47, 1026, 1316, 2608
Bsp13I TCCGGA 1 cut(s) 2536
Bsp1407I TGTACA 1 cut(s) 1005
Bsp1720I GCTNAGC 1 cut(s) 1637
BspACI CCGC 7 cut(s) 30, 120, 963, 1583, 1656, 1703, 2506
BspANI GGCC 6 cut(s) 357, 535, 786, 962, 1957, 2706
BspCNI CTCAG 9 cut(s) 42, 594, 600, 754, 1893, 2299, 2328, 2393, 2450
BspEI TCCGGA 1 cut(s) 2536
BspFNI CGCG 1 cut(s) 267
BspHI TCATGA 2 cut(s) 1554, 2215
BspMAI CTGCAG 2 cut(s) 394, 2808
BspMI ACCTGC 1 cut(s) 1436
BspOI GCTAGC 1 cut(s) 749
BspPI GGATC 9 cut(s) 62, 168, 177, 753, 766, 1193, 1294, 1360, 2763
BspT107I GGYRCC 1 cut(s) 1760
BsrBI CCGCTC 1 cut(s) 2508
BsrDI GCAATG 2 cut(s) 945, 1288
BsrFI RCCGGY 1 cut(s) 122
BsrGI TGTACA 1 cut(s) 1005
BsrI ACTGG 6 cut(s) 342, 359, 1049, 1203, 1514, 2678
BssAI RCCGGY 1 cut(s) 122
BssECI CCNNGG 9 cut(s) 156, 219, 647, 803, 979, 1488, 1489, 1529, 1764
BssNI GRCGYC 1 cut(s) 2883
BssT1I CCWWGG 3 cut(s) 803, 1529, 1764
Bst6I CTCTTC 3 cut(s) 505, 1530, 2379
BstACI GRCGYC 1 cut(s) 2883
BstAUI TGTACA 1 cut(s) 1005
BstBAI YACGTR 1 cut(s) 925
BstC8I GCNNGC 8 cut(s) 47, 94, 226, 747, 813, 960, 1082, 1086
BstEII GGTNACC 1 cut(s) 214
BstENI CCTNNNNNAGG 1 cut(s) 882
BstF5I GGATG 8 cut(s) 322, 427, 998, 1119, 1637, 1939, 2733, 2876
BstFNI CGCG 1 cut(s) 267
BstHHI GCGC 2 cut(s) 697, 2654
BstMAI GTCTC 4 cut(s) 534, 1323, 2224, 2381
BstMWI GCNNNNNNNGC 7 cut(s) 46, 134, 935, 1557, 1703, 2561, 2880
BstNSI RCATGY 5 cut(s) 605, 1882, 1888, 2765, 2968
BstPI GGTNACC 1 cut(s) 214
BstSFI CTRYAG 4 cut(s) 328, 390, 558, 2804
BstUI CGCG 1 cut(s) 267
BstV1I GCAGC 4 cut(s) 124, 391, 404, 2818
BstV2I GAAGAC 2 cut(s) 1902, 2559
BstX2I RGATCY 3 cut(s) 758, 2376, 2818
BstYI RGATCY 3 cut(s) 758, 2376, 2818
BsuRI GGCC 6 cut(s) 357, 535, 786, 962, 1957, 2706
BtsCI GGATG 8 cut(s) 322, 427, 998, 1119, 1637, 1939, 2733, 2876
BtsIMutI CAGTG 5 cut(s) 1187, 1210, 1657, 1931, 2500
BveI ACCTGC 1 cut(s) 1436
Cac8I GCNNGC 8 cut(s) 47, 94, 226, 747, 813, 960, 1082, 1086
CaiI CAGNNNCTG 3 cut(s) 398, 1697, 2036
CciI TCATGA 2 cut(s) 1554, 2215
CfoI GCGC 2 cut(s) 697, 2654
Cfr10I RCCGGY 1 cut(s) 122
Cfr13I GGNCC 9 cut(s) 356, 585, 785, 800, 860, 1209, 1425, 2112, 2206
CseI GACGC 4 cut(s) 117, 2553, 2798, 2872
CsiI ACCWGGT 1 cut(s) 907
Csp6I GTAC 5 cut(s) 1006, 1685, 1761, 2043, 2772
CspCI CAANNNNNGTGG 2 cut(s) 777, 812
CviQI GTAC 5 cut(s) 1006, 1685, 1761, 2043, 2772
DraI TTTAAA 1 cut(s) 1162
DraIII CACNNNGTG 1 cut(s) 2601
EaeI YGGCCR 3 cut(s) 533, 960, 1955
Eam1104I CTCTTC 3 cut(s) 505, 1530, 2379
EarI CTCTTC 3 cut(s) 505, 1530, 2379
EciI GGCGGA 1 cut(s) 109
Ecl136II GAGCTC 3 cut(s) 45, 1024, 1314
Eco130I CCWWGG 3 cut(s) 803, 1529, 1764
Eco147I AGGCCT 1 cut(s) 2706
Eco24I GRGCYC 3 cut(s) 47, 1026, 1316
Eco47I GGWCC 7 cut(s) 585, 800, 860, 1209, 1425, 2112, 2206
Eco53kI GAGCTC 3 cut(s) 45, 1024, 1314
Eco57I CTGAAG 5 cut(s) 230, 1366, 1802, 2388, 2718
Eco88I CYCGRG 1 cut(s) 1140
Eco91I GGTNACC 1 cut(s) 214
EcoICRI GAGCTC 3 cut(s) 45, 1024, 1314
EcoNI CCTNNNNNAGG 1 cut(s) 882
EcoO109I RGGNCCY 6 cut(s) 585, 800, 860, 1209, 1425, 2206
EcoO65I GGTNACC 1 cut(s) 214
EcoT14I CCWWGG 3 cut(s) 803, 1529, 1764
EcoT22I ATGCAT 1 cut(s) 2053
EcoT38I GRGCYC 3 cut(s) 47, 1026, 1316
ErhI CCWWGG 3 cut(s) 803, 1529, 1764
Esp3I CGTCTC 1 cut(s) 1323
FaqI GGGAC 5 cut(s) 571, 846, 1222, 1438, 2192
FauI CCCGC 1 cut(s) 2513
FblI GTMKAC 1 cut(s) 1538
Fnu4HI GCNGC 8 cut(s) 31, 138, 380, 393, 963, 1656, 1704, 2807
FokI GGATG 8 cut(s) 329, 414, 1005, 1126, 1644, 1946, 2740, 2883
FriOI GRGCYC 3 cut(s) 47, 1026, 1316
Fsp4HI GCNGC 8 cut(s) 31, 138, 380, 393, 963, 1656, 1704, 2807
FspBI CTAG 8 cut(s) 239, 746, 804, 948, 1041, 1173, 2322, 2474
GlaI GCGC 2 cut(s) 696, 2653
GluI GCNGC 8 cut(s) 31, 138, 380, 393, 963, 1656, 1704, 2807
GsaI CCCAGC 1 cut(s) 1113
GsuI CTGGAG 3 cut(s) 471, 867, 1032
HaeIII GGCC 6 cut(s) 357, 535, 786, 962, 1957, 2706
HapII CCGG 4 cut(s) 123, 1089, 2537, 2880
HgaI GACGC 4 cut(s) 117, 2553, 2798, 2872
HhaI GCGC 2 cut(s) 697, 2654
Hin1I GRCGYC 1 cut(s) 2883
Hin6I GCGC 2 cut(s) 695, 2652
HinP1I GCGC 2 cut(s) 695, 2652
HincII GTYRAC 1 cut(s) 1648
HindII GTYRAC 1 cut(s) 1648
HindIII AAGCTT 1 cut(s) 2669
HinfI GANTC 6 cut(s) 180, 527, 944, 2354, 2688, 2795
HpaII CCGG 4 cut(s) 123, 1089, 2537, 2880
HphI GGTGA 7 cut(s) 691, 897, 995, 1472, 1505, 2249, 2725
Hpy166II GTNNAC 3 cut(s) 953, 1539, 1648
Hpy8I GTNNAC 3 cut(s) 953, 1539, 1648
Hpy99I CGWCG 4 cut(s) 67, 88, 104, 137
HpyCH4IV ACGT 3 cut(s) 774, 924, 1945
HpyF10VI GCNNNNNNNGC 7 cut(s) 46, 134, 935, 1557, 1703, 2561, 2880
HpySE526I ACGT 3 cut(s) 774, 924, 1945
Hsp92I GRCGYC 1 cut(s) 2883
HspAI GCGC 2 cut(s) 695, 2652
KflI GGGWCCC 1 cut(s) 1209
Kpn2I TCCGGA 1 cut(s) 2536
KpnI GGTACC 1 cut(s) 1764
LmnI GCTCC 7 cut(s) 122, 1029, 1311, 1768, 2429, 2611, 2860
Lsp1109I GCAGC 4 cut(s) 124, 391, 404, 2818
MabI ACCWGGT 1 cut(s) 907
MaeI CTAG 8 cut(s) 239, 746, 804, 948, 1041, 1173, 2322, 2474
MaeII ACGT 3 cut(s) 774, 924, 1945
MaeIII GTNAC 9 cut(s) 214, 920, 983, 1135, 1219, 1745, 2294, 2399, 2782
MbiI CCGCTC 1 cut(s) 2508
MfeI CAATTG 2 cut(s) 1407, 2721
MflI RGATCY 3 cut(s) 758, 2376, 2818
MhlI GDGCHC 4 cut(s) 47, 1026, 1316, 2608
MlsI TGGCCA 1 cut(s) 1957
MluI ACGCGT 1 cut(s) 265
MluNI TGGCCA 1 cut(s) 1957
MlyI GAGTC 2 cut(s) 536, 953
MmeI TCCRAC 3 cut(s) 348, 849, 2306
Mox20I TGGCCA 1 cut(s) 1957
Mph1103I ATGCAT 1 cut(s) 2053
MroI TCCGGA 1 cut(s) 2536
MroXI GAANNNNTTC 1 cut(s) 2016
MscI TGGCCA 1 cut(s) 1957
MseI TTAA 8 cut(s) 192, 771, 1161, 1248, 1986, 2069, 2622, 2989
MslI CAYNNNNRTG 2 cut(s) 594, 1883
Msp20I TGGCCA 1 cut(s) 1957
MspA1I CMGCKG 4 cut(s) 563, 958, 965, 1658
MspI CCGG 4 cut(s) 123, 1089, 2537, 2880
MunI CAATTG 2 cut(s) 1407, 2721
Mva1269I GAATGC 2 cut(s) 94, 2467
MvnI CGCG 1 cut(s) 267
MwoI GCNNNNNNNGC 7 cut(s) 46, 134, 935, 1557, 1703, 2561, 2880
NheI GCTAGC 1 cut(s) 745
NmeAIII GCCGAG 3 cut(s) 66, 511, 2571
NmuCI GTSAC 3 cut(s) 983, 2399, 2782
NsiI ATGCAT 1 cut(s) 2053
NspI RCATGY 5 cut(s) 605, 1882, 1888, 2765, 2968
PagI TCATGA 2 cut(s) 1554, 2215
PasI CCCWGGG 1 cut(s) 1489
PceI AGGCCT 1 cut(s) 2706
PcsI WCGNNNNNNNCGW 1 cut(s) 108
PctI GAATGC 2 cut(s) 94, 2467
PdmI GAANNNNTTC 1 cut(s) 2016
PfeI GAWTC 4 cut(s) 180, 2354, 2688, 2795
PfoI TCCNGGA 4 cut(s) 883, 1368, 2114, 2835
PkrI GCNGC 8 cut(s) 32, 139, 381, 394, 964, 1657, 1705, 2808
PleI GAGTC 2 cut(s) 535, 952
PpsI GAGTC 2 cut(s) 535, 952
Ppu21I YACGTR 1 cut(s) 925
PpuMI RGGWCCY 6 cut(s) 585, 800, 860, 1209, 1425, 2206
PshBI ATTAAT 2 cut(s) 192, 1248
Psp124BI GAGCTC 3 cut(s) 47, 1026, 1316
Psp5II RGGWCCY 6 cut(s) 585, 800, 860, 1209, 1425, 2206
PspEI GGTNACC 1 cut(s) 214
PspFI CCCAGC 1 cut(s) 1109
PspPI GGNCC 9 cut(s) 356, 585, 785, 800, 860, 1209, 1425, 2112, 2206
PspPPI RGGWCCY 6 cut(s) 585, 800, 860, 1209, 1425, 2206
PstI CTGCAG 2 cut(s) 394, 2808
PstNI CAGNNNCTG 3 cut(s) 398, 1697, 2036
PsuI RGATCY 3 cut(s) 758, 2376, 2818
PvuII CAGCTG 2 cut(s) 563, 958
RsaI GTAC 5 cut(s) 1007, 1686, 1762, 2044, 2773
RsaNI GTAC 5 cut(s) 1006, 1685, 1761, 2043, 2772
RseI CAYNNNNRTG 2 cut(s) 594, 1883
SacI GAGCTC 3 cut(s) 47, 1026, 1316
SaqAI TTAA 8 cut(s) 192, 771, 1161, 1248, 1986, 2069, 2622, 2989
SatI GCNGC 8 cut(s) 31, 138, 380, 393, 963, 1656, 1704, 2807
Sau96I GGNCC 9 cut(s) 356, 585, 785, 800, 860, 1209, 1425, 2112, 2206
ScaI AGTACT 1 cut(s) 2773
SchI GAGTC 2 cut(s) 536, 953
SduI GDGCHC 4 cut(s) 47, 1026, 1316, 2608
SexAI ACCWGGT 1 cut(s) 907
SfcI CTRYAG 4 cut(s) 328, 390, 558, 2804
SgrAI CRCCGGYG 1 cut(s) 122
SinI GGWCC 7 cut(s) 585, 800, 860, 1209, 1425, 2112, 2206
SmiMI CAYNNNNRTG 2 cut(s) 594, 1883
SmlI CTYRAG 3 cut(s) 71, 1750, 2637
SmoI CTYRAG 3 cut(s) 71, 1750, 2637
SpeI ACTAGT 1 cut(s) 1172
SseBI AGGCCT 1 cut(s) 2706
SsiI CCGC 7 cut(s) 30, 120, 963, 1583, 1656, 1703, 2506
SspI AATATT 2 cut(s) 1252, 2122
SspMI CTAG 8 cut(s) 239, 746, 804, 948, 1041, 1173, 2322, 2474
SstI GAGCTC 3 cut(s) 47, 1026, 1316
StuI AGGCCT 1 cut(s) 2706
StyI CCWWGG 3 cut(s) 803, 1529, 1764
TaiI ACGT 3 cut(s) 777, 927, 1948
TaqI TCGA 4 cut(s) 83, 312, 705, 2481
TaqII GACCGA 1 cut(s) 606
TatI WGTACW 2 cut(s) 1005, 2771
TauI GCSGC 4 cut(s) 33, 965, 1658, 1706
TfiI GAWTC 4 cut(s) 180, 2354, 2688, 2795
Tru1I TTAA 8 cut(s) 192, 771, 1161, 1248, 1986, 2069, 2622, 2989
Tru9I TTAA 8 cut(s) 192, 771, 1161, 1248, 1986, 2069, 2622, 2989
TscAI CASTG 5 cut(s) 1194, 1210, 1657, 1931, 2500
TseFI GTSAC 3 cut(s) 983, 2399, 2782
TseI GCWGC 4 cut(s) 137, 379, 392, 2806
Tsp45I GTSAC 3 cut(s) 983, 2399, 2782
TspGWI ACGGA 2 cut(s) 121, 2768
TspRI CASTG 5 cut(s) 1194, 1210, 1657, 1931, 2500
VpaK11BI GGWCC 7 cut(s) 585, 800, 860, 1209, 1425, 2112, 2206
VspI ATTAAT 2 cut(s) 192, 1248
XagI CCTNNNNNAGG 1 cut(s) 882
XapI RAATTY 3 cut(s) 713, 1060, 2332
XceI RCATGY 5 cut(s) 605, 1882, 1888, 2765, 2968
XmaJI CCTAGG 1 cut(s) 803
XmiI GTMKAC 1 cut(s) 1538
XmnI GAANNNNTTC 1 cut(s) 2016
XspI CTAG 8 cut(s) 239, 746, 804, 948, 1041, 1173, 2322, 2474
ZrmI AGTACT 1 cut(s) 2773
Zsp2I ATGCAT 1 cut(s) 2053
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.