pycom03g21730

Autophagy-related protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
OG0006636
Physical Location & Seq
Forward (+)
21978485 .. 21983534
5050 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom03g21730.8

Sequence Viewer

Length: 3024 bp
ATGAAGAAGAGTAAGGGGAAGAACAATGGCTTGTTGCCGAACTCGCTTAGGATTATTTCGTCGTGCCTTAAAACTGTGTCGACGAATGCGAGCACCGTCGCTTCTACTGTTCGCTCTGCCGGTGCCTCTGTAGCTGCTTCGATTTCTGCTTCCGAGGACCAGAAGGATCAGGTAACCTGGGCTGGCTTTGACAGGCTAGAGCTTAGTCACTCTACCTTCAAGCATGTTCTCTTACTTGGTTATCAGAATGGATTTCAAGTTTTCGATATGGAAGATGCCTCTAATTTCAGTGAACTAGTTTCAAAGCGCGATGGCCCGGTTTCGTTCTTACAGATGCAGCCCTTTCCTGCTGCATCTGATGGTAACCAAGGGTTCAGAACCGCGCATCCTTTGCTGCTGGTTGTTGCTGGAGATGACACTAATGGTGCGGGAATTGTTCAGAATACTACCCACTTGGGAGGGACAGGCAGAGATAGTAATTTGGAGTCTCGGCCGGGGAACCCTGTCAGCTCTGCTACAGCTGTTCGGTTTTACTCCCTTAGGTCCCAAGGTTACGTACACGTTCTGAGGTTTCGGTCTGCTGTGTGTATGATTAGATGCAGTACTCGGATAGTAGCTGTGGGTCTTGCAAGTCAAATTTACTGCTTTGACGCACTCACCCTCGAAAACAGATTTAGTGTTCTTACCTATCCTGTTCCTCAGCTAGCAGGGCAAGGATCAAATGGGTTTAATGTTGGTTACGGCCCAATGGCTGTGGGTCCTAGGTGGTTAGCCTATGCTTCCAACAGCCCTCTAGTGTCAAATGCAAGTTGCTTAGGCCCCCAAAACCTCACTCCTTCTCCCGGAGTTAGTCCATCCACATCCCCTGGTAATGGTAGTTATGTGGCTCGTTATGCAGTGGAGTCTAGTAAACAGTTGGCTGCTGGAATAATTAACCTGGGTGACATGGGATGCAAAACCTTGTATAAATACTGTCAAGAGATGCTCCCTGATGGATCTAACTCTCCAATATCGTCAAATTCAGGCTGGAAAGTTAGCAGGCATGCAGGCACAGAAATGGATAATGCAGGGATGGTTGTTGTCAAAGATTTTATTACCCGAGCTGTTATATCGCAGTTTAAAGCTCATACTAGTCCAATATCTGCACTGTGTTTCGATCCTAGTGGGACCCTTCTTGTTACTGCCTCAGTATATGGAAATAATATTAATATTTTTCGGATAATGCCTTCATGCAAACGCAGTGGATCAGGTGTTCAAAACTTCGATTGGAGCTCTTCTCATGTGCATCTTTACAAACTGCATCGTGGAATATTATCAGCTATGATCCAGGACATTTGCTTTAGTCATTACAGTCAGTGGGTTGCTATTGTTTCGTCCAAGGGGACCTGCCATGTTTTTGTTTTATCCCCTTTTGGTGGTGATGCTGGATTTCGGCTTCCTAATAATCACGGTGAAGAACCCTCGCTGTGCCCAATTTTAACCCTACCTTGGTGGTCTGCTTCTTCTTGCATCATTAATCAACAATCTTTTCCACCGCCACCACCTGTTGCTCTTTCCGTGGTGAGCAGGATAAAGTACAGTAGTTTTGGATGGCTTAGCACAGTCAACAATGCCACTGCTTCAGCAACAGGGAAGGTCTTTGTGCCTTCTGGTGCTGTTGCTGCAGTTTTTCATAATTCTTTATGTCAGAGACTTCATCACAGTAACTCAAGGAAGAGTACCTTGGAACATCTATTAGTTTATACTCCGTCGGGTCATGTAGTTCAACATGAACTTCAGCCAAGGATAGGGGTAGAACAGAGTCATAGCGGTCTAAGTACCCAGGCAGCCACGTCTATGCATATGCAGGACGAAGAGCTGAGATTGAAAGTTGAACCTATTCAATGGTGGGACGTGTGTAGGCGGTCAGACTGGCCAGAAAGGGAGGACAGTAGTTTTGGTACCACATCCGATAGACAAGAGGTGGCTGACATAATCCAGAACAAATCAGGTTCTGATGGTACTCATAGAATGGAATCTCTAGACTTAAATGGTGCTGTTGGGGGCGAAAGGAGACTGGAAACTTATTCTGGGAAGGTCCATGATAGGTCCCACTGGTACCTCTCTAATGCAGAGGTGCAAATCAGTTCTTTGAGATTACCAATATGGCAAAAGTCTAAGATTTGTTTTTACAGGATGGGCTGTCCAAGAGCTGATAGTTTCACCGGTGGAGAGTTTGAAACTGAAAAGGTCCCTGTTCATGAAATTGAAATGAGACAGAAGGATTTGTTGCCTGTTTTTGAGCAGTTCCATTTCATCAAATCAAGCTGGGATGACAGAAGCCTTGCTGGTGGGACGTATCCCAGTCATACTTTGCCACCTCATCAAGCTGAAGACAAGACCTTGGAAGAGACTGTTATATGTCACTCGAAGCCTGCATCACTTAGCTCCACTGAAAGCTCAGATGGGGGTTCGTCAAGAAGAATTGAGCATTTTCTCGATTTGATCGATCAAATAAACAATGAGAAGCCCCGCACAACAGTGTATCAGACCTTGGATGGCGCGGAAAGAAGAGCCAATACAATTGTTGAGCCTTCGTTAGAAAACCATGTATCTTTCAGCATTCGGCGTACTCCATCTGAACATTTTAAGAACATCGATTCTCAAGTCAACGGCTGTGTCACCAACGGTTTGCCTGTGTTAGAAAGTAAATTGAGTTCAGGAGGAAGAGTTTCTGCTGAAGAAGGCCCAGCATTTAAAGCAATTGGGGTCAGGGAGGTCTCGGTTTTATACTCTGATCAACATGCTTCGAGTACCGATATCGTGGCAGAGGGGGCTTCCACTTTGCAGCATCCGATAGATCTTTCACAGTTTTTCCAGGAGGAGCATTGTAATGCACTGGAGAAGAATGGATGCAATGGGCTAACTGAAGTCGTAGGTGACGATGTTGACAGCGACAGTAGCCACTGCGATAAGGTGAAACCCGAAGACGCGGAAGATGGTGAAATGCTTGGAGGCATATTTGCCTTTTCTGATGGAGGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000226 GO:0000785 GO:0001952 GO:0001953 GO:0003674 GO:0003682 GO:0005102 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005694 GO:0005730 GO:0005737 GO:0005856 GO:0005874 GO:0005881 GO:0006355 GO:0006357 GO:0006810 GO:0006950 GO:0006996 GO:0007010 GO:0007017 GO:0007030 GO:0007162 GO:0007275 GO:0008047 GO:0008092 GO:0008134 GO:0008150 GO:0009605 GO:0009653 GO:0009889 GO:0009891 GO:0009893 GO:0009987 GO:0009991 GO:0010256 GO:0010468 GO:0010556 GO:0010557 GO:0010594 GO:0010595 GO:0010604 GO:0010628 GO:0010632 GO:0010634 GO:0010638 GO:0010698 GO:0010810 GO:0010812 GO:0015630 GO:0015631 GO:0016043 GO:0016192 GO:0019219 GO:0019222 GO:0019899 GO:0030155 GO:0030234 GO:0030334 GO:0030335 GO:0031023 GO:0031252 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031344 GO:0031346 GO:0031667 GO:0031974 GO:0031981 GO:0032386 GO:0032388 GO:0032501 GO:0032502 GO:0032878 GO:0032879 GO:0032880 GO:0032956 GO:0032970 GO:0033043 GO:0033157 GO:0034260 GO:0035035 GO:0035148 GO:0035239 GO:0035257 GO:0035295 GO:0035327 GO:0040012 GO:0040017 GO:0042221 GO:0042393 GO:0042594 GO:0043085 GO:0043086 GO:0043087 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043547 GO:0043627 GO:0044087 GO:0044089 GO:0044092 GO:0044093 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044430 GO:0044444 GO:0044446 GO:0044464 GO:0045111 GO:0045893 GO:0045935 GO:0045944 GO:0048487 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048646 GO:0048856 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051049 GO:0051050 GO:0051128 GO:0051129 GO:0051130 GO:0051171 GO:0051173 GO:0051179 GO:0051222 GO:0051223 GO:0051234 GO:0051239 GO:0051240 GO:0051252 GO:0051254 GO:0051270 GO:0051272 GO:0051336 GO:0051345 GO:0051346 GO:0051427 GO:0051489 GO:0051491 GO:0051493 GO:0051495 GO:0051716 GO:0051893 GO:0051895 GO:0060255 GO:0060341 GO:0060491 GO:0065007 GO:0065009 GO:0070013 GO:0070201 GO:0070887 GO:0071391 GO:0071840 GO:0080090 GO:0090087 GO:0090109 GO:0090316 GO:0090630 GO:0098772 GO:0099080 GO:0099081 GO:0099512 GO:0099513 GO:0120032 GO:0120034 GO:0120035 GO:1901888 GO:1901889 GO:1902680 GO:1903391 GO:1903392 GO:1903506 GO:1903508 GO:1903827 GO:1903829 GO:1904951 GO:2000112 GO:2000114 GO:2000145 GO:2000147 GO:2000249 GO:2000251 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
BCAS3 WD40

Protein Analysis

1008

Amino Acids

109.44

Weight (kDa)

6.18

Isoelectric Point (pI)

44.86

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1394
Acc65I GGTACC 2 cut(s) 1940, 2097
AccB1I GGYRCC 3 cut(s) 122, 1940, 2097
AccI GTMKAC 1 cut(s) 80
AccII CGCG 4 cut(s) 309, 383, 2543, 2972
AciI CCGC 8 cut(s) 381, 428, 1535, 1809, 1903, 2512, 2543, 2972
AclWI GGATC 6 cut(s) 174, 724, 1003, 1151, 1252, 1318
AcoI YGGCCR 2 cut(s) 491, 1913
AcsI RAATTY 2 cut(s) 636, 1018
AcuI CTGAAG 5 cut(s) 1605, 1760, 2391, 2739, 2928
AfiI CCNNNNNNNGG 5 cut(s) 842, 872, 1415, 1488, 1787
AflIII ACRYGT 2 cut(s) 559, 1893
AgeI ACCGGT 1 cut(s) 2204
AhlI ACTAGT 2 cut(s) 295, 1130
AjiI CACGTC 2 cut(s) 1833, 1894
AjnI CCWGG 6 cut(s) 176, 865, 936, 1326, 1821, 2856
AjuI GAANNNNNNNTTGG 2 cut(s) 1706, 1738
AleI CACNNNNGTG 1 cut(s) 2519
AloI GAACNNNNNNTCC 4 cut(s) 370, 402, 1236, 1268
Alw21I GWGCWC 2 cut(s) 95, 1274
Alw26I GTCTC 6 cut(s) 492, 1684, 2047, 2248, 2384, 2764
AlwI GGATC 6 cut(s) 174, 724, 1003, 1151, 1252, 1318
AlwNI CAGNNNCTG 1 cut(s) 1994
Ama87I CYCGRG 1 cut(s) 1098
AoxI GGCC 6 cut(s) 313, 491, 742, 817, 1913, 2725
ApeKI GCWGC 8 cut(s) 134, 337, 350, 394, 920, 1661, 1826, 2827
ApoI RAATTY 2 cut(s) 636, 1018
ArsI GACNNNNNNTTYG 2 cut(s) 1919, 1951
AseI ATTAAT 2 cut(s) 1206, 1515
AsiGI ACCGGT 1 cut(s) 2204
Asp700I GAANNNNTTC 2 cut(s) 1878, 2710
Asp718I GGTACC 2 cut(s) 1940, 2097
AspA2I CCTAGG 1 cut(s) 761
AspLEI GCGC 3 cut(s) 309, 385, 2543
AsuC2I CCSGG 3 cut(s) 317, 495, 843
AsuNHI GCTAGC 1 cut(s) 703
AvaI CYCGRG 1 cut(s) 1098
AvaII GGWCC 8 cut(s) 157, 543, 758, 1167, 1383, 2077, 2088, 2230
AvrII CCTAGG 1 cut(s) 761
AxyI CCTNAGG 1 cut(s) 539
BaeGI GKGCMC 1 cut(s) 1472
BalI TGGCCA 1 cut(s) 1915
BanI GGYRCC 3 cut(s) 122, 1940, 2097
BanII GRGCYC 1 cut(s) 1274
BarI GAAGNNNNNNTAC 2 cut(s) 2542, 2574
BbsI GAAGAC 2 cut(s) 2379, 2973
Bbv12I GWGCWC 2 cut(s) 95, 1274
BbvCI CCTCAGC 1 cut(s) 699
BbvI GCAGC 8 cut(s) 121, 337, 349, 381, 907, 1648, 1838, 2839
BceAI ACGGC 2 cut(s) 757, 2668
BciT130I CCWGG 6 cut(s) 178, 867, 938, 1328, 1823, 2858
BciVI GTATCC 1 cut(s) 2349
BclI TGATCA 1 cut(s) 2776
BcnI CCSGG 3 cut(s) 317, 495, 843
BcoDI GTCTC 6 cut(s) 492, 1684, 2047, 2248, 2384, 2764
BcuI ACTAGT 2 cut(s) 295, 1130
BfaI CTAG 9 cut(s) 197, 296, 704, 762, 794, 906, 1131, 1161, 2021
BfmI CTRYAG 3 cut(s) 129, 516, 1662
BfuAI ACCTGC 1 cut(s) 1394
BfuI GTATCC 1 cut(s) 2349
BglII AGATCT 1 cut(s) 2839
BisI GCNGC 8 cut(s) 135, 338, 351, 395, 921, 1662, 1827, 2828
BlnI CCTAGG 1 cut(s) 761
BlpI GCTNAGC 1 cut(s) 1595
BlsI GCNGC 8 cut(s) 136, 339, 352, 396, 922, 1663, 1828, 2829
BmcAI AGTACT 1 cut(s) 604
Bme1390I CCNGG 9 cut(s) 178, 317, 495, 843, 867, 938, 1328, 1823, 2858
Bme18I GGWCC 8 cut(s) 157, 543, 758, 1167, 1383, 2077, 2088, 2230
BmeT110I CYCGRG 1 cut(s) 1098
BmgBI CACGTC 2 cut(s) 1833, 1894
BmrFI CCNGG 9 cut(s) 178, 317, 495, 843, 867, 938, 1328, 1823, 2858
BmrI ACTGGG 1 cut(s) 2337
BmtI GCTAGC 1 cut(s) 707
BmuI ACTGGG 1 cut(s) 2337
BpiI GAAGAC 2 cut(s) 2379, 2973
BplI GAGNNNNNCTC 2 cut(s) 1261, 1293
BpmI CTGGAG 2 cut(s) 429, 2900
Bpu10I CCTNAGC 3 cut(s) 47, 699, 814
Bpu1102I GCTNAGC 1 cut(s) 1595
BpuEI CTTGAG 2 cut(s) 1693, 2628
BpuMI CCSGG 3 cut(s) 317, 495, 843
Bsa29I ATCGAT 2 cut(s) 2487, 2637
BsaAI YACGTR 1 cut(s) 556
BsaI GGTCTC 1 cut(s) 2764
BsaWI WCCGGW 1 cut(s) 2204
BsaXI ACNNNNNCTCC 4 cut(s) 823, 853, 2694, 2724
Bsc4I CCNNNNNNNGG 5 cut(s) 842, 872, 1415, 1488, 1787
Bse118I RCCGGY 2 cut(s) 119, 2204
Bse1I ACTGG 5 cut(s) 1916, 2061, 2099, 2343, 2883
Bse21I CCTNAGG 1 cut(s) 539
Bse3DI GCAATG 1 cut(s) 2902
BseBI CCWGG 6 cut(s) 178, 867, 938, 1328, 1823, 2858
BseCI ATCGAT 2 cut(s) 2487, 2637
BseLI CCNNNNNNNGG 5 cut(s) 842, 872, 1415, 1488, 1787
BseMI GCAATG 1 cut(s) 2902
BseMII CTCAG 5 cut(s) 557, 713, 1200, 1850, 2454
BseNI ACTGG 5 cut(s) 1916, 2061, 2099, 2343, 2883
BseRI GAGGAG 1 cut(s) 2876
BseSI GKGCMC 1 cut(s) 1472
BseX3I CGGCCG 1 cut(s) 491
BseXI GCAGC 8 cut(s) 121, 337, 349, 381, 907, 1648, 1838, 2839
BseYI CCCAGC 2 cut(s) 2307, 2728
BsgI GTGCAG 1 cut(s) 1128
Bsh1236I CGCG 4 cut(s) 309, 383, 2543, 2972
Bsh1285I CGRYCG 1 cut(s) 494
BshFI GGCC 6 cut(s) 315, 493, 744, 819, 1915, 2727
BshNI GGYRCC 3 cut(s) 122, 1940, 2097
BshTI ACCGGT 1 cut(s) 2204
BshVI ATCGAT 2 cut(s) 2487, 2637
BsiEI CGRYCG 1 cut(s) 494
BsiHKAI GWGCWC 2 cut(s) 95, 1274
BsiHKCI CYCGRG 1 cut(s) 1098
BsiSI CCGG 5 cut(s) 120, 317, 494, 843, 2205
BslFI GGGAC 8 cut(s) 475, 529, 1180, 1396, 1904, 2074, 2216, 2347
BslI CCNNNNNNNGG 5 cut(s) 842, 872, 1415, 1488, 1787
BsmAI GTCTC 6 cut(s) 492, 1684, 2047, 2248, 2384, 2764
BsmFI GGGAC 8 cut(s) 475, 529, 1180, 1396, 1904, 2074, 2216, 2347
BsmI GAATGC 2 cut(s) 91, 2601
BsnI GGCC 6 cut(s) 315, 493, 744, 819, 1915, 2727
Bso31I GGTCTC 1 cut(s) 2764
BsoBI CYCGRG 1 cut(s) 1098
Bsp1286I GDGCHC 3 cut(s) 95, 1274, 1472
Bsp1720I GCTNAGC 1 cut(s) 1595
BspACI CCGC 8 cut(s) 381, 428, 1535, 1809, 1903, 2512, 2543, 2972
BspANI GGCC 6 cut(s) 315, 493, 744, 819, 1915, 2727
BspCNI CTCAG 5 cut(s) 558, 712, 1199, 1851, 2453
BspDI ATCGAT 2 cut(s) 2487, 2637
BspFNI CGCG 4 cut(s) 309, 383, 2543, 2972
BspHI TCATGA 1 cut(s) 2239
BspMAI CTGCAG 1 cut(s) 1666
BspMI ACCTGC 1 cut(s) 1394
BspOI GCTAGC 1 cut(s) 707
BspPI GGATC 6 cut(s) 174, 724, 1003, 1151, 1252, 1318
BspQI GCTCTTC 3 cut(s) 1279, 1848, 2545
BspT107I GGYRCC 3 cut(s) 122, 1940, 2097
BspTNI GGTCTC 1 cut(s) 2764
BsrDI GCAATG 1 cut(s) 2902
BsrFI RCCGGY 2 cut(s) 119, 2204
BsrI ACTGG 5 cut(s) 1916, 2061, 2099, 2343, 2883
BssAI RCCGGY 2 cut(s) 119, 2204
BssT1I CCWWGG 9 cut(s) 367, 547, 761, 1377, 1487, 1722, 1781, 2382, 2532
Bst2UI CCWGG 6 cut(s) 178, 867, 938, 1328, 1823, 2858
Bst6I CTCTTC 7 cut(s) 2, 1279, 1709, 1848, 2382, 2545, 2701
BstAPI GCANNNNNTGC 1 cut(s) 391
BstBAI YACGTR 1 cut(s) 556
BstC8I GCNNGC 7 cut(s) 91, 184, 705, 1040, 1044, 1048, 2415
BstDSI CCRYGG 1 cut(s) 1557
BstEII GGTNACC 2 cut(s) 172, 362
BstFNI CGCG 4 cut(s) 309, 383, 2543, 2972
BstHHI GCGC 3 cut(s) 309, 385, 2543
BstMAI GTCTC 6 cut(s) 492, 1684, 2047, 2248, 2384, 2764
BstMCI CGRYCG 1 cut(s) 494
BstMWI GCNNNNNNNGC 9 cut(s) 43, 131, 391, 709, 893, 1661, 2738, 2813, 2940
BstNI CCWGG 6 cut(s) 178, 867, 938, 1328, 1823, 2858
BstNSI RCATGY 3 cut(s) 227, 1046, 2786
BstPI GGTNACC 2 cut(s) 172, 362
BstSCI CCNGG 9 cut(s) 176, 315, 493, 841, 865, 936, 1326, 1821, 2856
BstSFI CTRYAG 3 cut(s) 129, 516, 1662
BstSLI GKGCMC 1 cut(s) 1472
BstSNI TACGTA 1 cut(s) 556
BstUI CGCG 4 cut(s) 309, 383, 2543, 2972
BstV1I GCAGC 8 cut(s) 121, 337, 349, 381, 907, 1648, 1838, 2839
BstV2I GAAGAC 2 cut(s) 2379, 2973
BstX2I RGATCY 2 cut(s) 995, 2839
BstYI RGATCY 2 cut(s) 995, 2839
BstZI CGGCCG 1 cut(s) 491
Bsu15I ATCGAT 2 cut(s) 2487, 2637
Bsu36I CCTNAGG 1 cut(s) 539
BsuI GTATCC 1 cut(s) 2349
BsuRI GGCC 6 cut(s) 315, 493, 744, 819, 1915, 2727
BsuTUI ATCGAT 2 cut(s) 2487, 2637
BtgI CCRYGG 1 cut(s) 1557
BtgZI GCGATG 1 cut(s) 324
BtrI CACGTC 2 cut(s) 1833, 1894
BtsI GCAGTG 4 cut(s) 903, 1246, 1614, 2944
BveI ACCTGC 1 cut(s) 1394
Cac8I GCNNGC 7 cut(s) 91, 184, 705, 1040, 1044, 1048, 2415
CaiI CAGNNNCTG 1 cut(s) 1994
CciI TCATGA 1 cut(s) 2239
CfoI GCGC 3 cut(s) 309, 385, 2543
Cfr10I RCCGGY 2 cut(s) 119, 2204
ClaI ATCGAT 2 cut(s) 2487, 2637
CseI GACGC 2 cut(s) 659, 2978
CspAI ACCGGT 1 cut(s) 2204
CspCI CAANNNNNGTGG 4 cut(s) 735, 770, 1222, 1257
DraI TTTAAA 2 cut(s) 1120, 2737
EaeI YGGCCR 2 cut(s) 491, 1913
EagI CGGCCG 1 cut(s) 491
Eam1104I CTCTTC 7 cut(s) 2, 1279, 1709, 1848, 2382, 2545, 2701
EarI CTCTTC 7 cut(s) 2, 1279, 1709, 1848, 2382, 2545, 2701
Ecl136II GAGCTC 1 cut(s) 1272
EclXI CGGCCG 1 cut(s) 491
Eco105I TACGTA 1 cut(s) 556
Eco130I CCWWGG 9 cut(s) 367, 547, 761, 1377, 1487, 1722, 1781, 2382, 2532
Eco24I GRGCYC 1 cut(s) 1274
Eco31I GGTCTC 1 cut(s) 2764
Eco32I GATATC 1 cut(s) 2800
Eco47I GGWCC 8 cut(s) 157, 543, 758, 1167, 1383, 2077, 2088, 2230
Eco52I CGGCCG 1 cut(s) 491
Eco53kI GAGCTC 1 cut(s) 1272
Eco57I CTGAAG 5 cut(s) 1605, 1760, 2391, 2739, 2928
Eco81I CCTNAGG 1 cut(s) 539
Eco88I CYCGRG 1 cut(s) 1098
Eco91I GGTNACC 2 cut(s) 172, 362
EcoICRI GAGCTC 1 cut(s) 1272
EcoO109I RGGNCCY 7 cut(s) 543, 758, 818, 1167, 1383, 2088, 2230
EcoO65I GGTNACC 2 cut(s) 172, 362
EcoRII CCWGG 6 cut(s) 176, 865, 936, 1326, 1821, 2856
EcoRV GATATC 1 cut(s) 2800
EcoT14I CCWWGG 9 cut(s) 367, 547, 761, 1377, 1487, 1722, 1781, 2382, 2532
EcoT22I ATGCAT 1 cut(s) 1842
EcoT38I GRGCYC 1 cut(s) 1274
ErhI CCWWGG 9 cut(s) 367, 547, 761, 1377, 1487, 1722, 1781, 2382, 2532
FalI AAGNNNNNCTT 2 cut(s) 1706, 1738
FaqI GGGAC 8 cut(s) 475, 529, 1180, 1396, 1904, 2074, 2216, 2347
FauI CCCGC 2 cut(s) 421, 2519
FauNDI CATATG 1 cut(s) 1842
FbaI TGATCA 1 cut(s) 2776
FblI GTMKAC 1 cut(s) 80
Fnu4HI GCNGC 8 cut(s) 135, 338, 351, 395, 921, 1662, 1827, 2828
FriOI GRGCYC 1 cut(s) 1274
Fsp4HI GCNGC 8 cut(s) 135, 338, 351, 395, 921, 1662, 1827, 2828
FspBI CTAG 9 cut(s) 197, 296, 704, 762, 794, 906, 1131, 1161, 2021
GlaI GCGC 3 cut(s) 308, 384, 2542
GluI GCNGC 8 cut(s) 135, 338, 351, 395, 921, 1662, 1827, 2828
GsaI CCCAGC 2 cut(s) 2311, 2732
GsuI CTGGAG 2 cut(s) 429, 2900
HaeIII GGCC 6 cut(s) 315, 493, 744, 819, 1915, 2727
HapII CCGG 5 cut(s) 120, 317, 494, 843, 2205
HgaI GACGC 2 cut(s) 659, 2978
HhaI GCGC 3 cut(s) 309, 385, 2543
Hin6I GCGC 3 cut(s) 307, 383, 2541
HinP1I GCGC 3 cut(s) 307, 383, 2541
HincII GTYRAC 4 cut(s) 81, 1606, 2650, 2929
HindII GTYRAC 4 cut(s) 81, 1606, 2650, 2929
HinfI GANTC 5 cut(s) 485, 902, 1801, 2015, 2639
HpaII CCGG 5 cut(s) 120, 317, 494, 843, 2205
Hpy166II GTNNAC 7 cut(s) 81, 293, 559, 911, 1606, 2650, 2929
Hpy188III TCNNGA 7 cut(s) 977, 1978, 2021, 2240, 2457, 2477, 2700
Hpy8I GTNNAC 7 cut(s) 81, 293, 559, 911, 1606, 2650, 2929
Hpy99I CGWCG 4 cut(s) 64, 85, 101, 1753
HpyCH4IV ACGT 5 cut(s) 555, 561, 1832, 1893, 2336
HpyF10VI GCNNNNNNNGC 9 cut(s) 43, 131, 391, 709, 893, 1661, 2738, 2813, 2940
HpySE526I ACGT 5 cut(s) 555, 561, 1832, 1893, 2336
HspAI GCGC 3 cut(s) 307, 383, 2541
KflI GGGWCCC 1 cut(s) 1167
KpnI GGTACC 2 cut(s) 1944, 2101
Ksp22I TGATCA 1 cut(s) 2776
LguI GCTCTTC 3 cut(s) 1279, 1848, 2545
LmnI GCTCC 4 cut(s) 990, 1269, 2432, 2863
Lsp1109I GCAGC 8 cut(s) 121, 337, 349, 381, 907, 1648, 1838, 2839
MaeI CTAG 9 cut(s) 197, 296, 704, 762, 794, 906, 1131, 1161, 2021
MaeII ACGT 5 cut(s) 555, 561, 1832, 1893, 2336
MfeI CAATTG 2 cut(s) 2562, 2742
MflI RGATCY 2 cut(s) 995, 2839
MhlI GDGCHC 3 cut(s) 95, 1274, 1472
MlsI TGGCCA 1 cut(s) 1915
MluNI TGGCCA 1 cut(s) 1915
MlyI GAGTC 3 cut(s) 494, 911, 1810
MmeI TCCRAC 1 cut(s) 807
Mox20I TGGCCA 1 cut(s) 1915
Mph1103I ATGCAT 1 cut(s) 1842
MroXI GAANNNNTTC 2 cut(s) 1878, 2710
MscI TGGCCA 1 cut(s) 1915
MslI CAYNNNNRTG 4 cut(s) 1055, 1835, 2519, 2871
Msp20I TGGCCA 1 cut(s) 1915
MspA1I CMGCKG 1 cut(s) 521
MspI CCGG 5 cut(s) 120, 317, 494, 843, 2205
MspR9I CCNGG 9 cut(s) 178, 317, 495, 843, 867, 938, 1328, 1823, 2858
MunI CAATTG 2 cut(s) 2562, 2742
Mva1269I GAATGC 2 cut(s) 91, 2601
MvaI CCWGG 6 cut(s) 178, 867, 938, 1328, 1823, 2858
MvnI CGCG 4 cut(s) 309, 383, 2543, 2972
MwoI GCNNNNNNNGC 9 cut(s) 43, 131, 391, 709, 893, 1661, 2738, 2813, 2940
NciI CCSGG 3 cut(s) 317, 495, 843
NdeI CATATG 1 cut(s) 1842
NheI GCTAGC 1 cut(s) 703
NmeAIII GCCGAG 1 cut(s) 469
NmuCI GTSAC 5 cut(s) 206, 941, 2402, 2659, 2918
NsiI ATGCAT 1 cut(s) 1842
NspI RCATGY 3 cut(s) 227, 1046, 2786
OliI CACNNNNGTG 1 cut(s) 2519
PaeI GCATGC 1 cut(s) 1046
PagI TCATGA 1 cut(s) 2239
PciSI GCTCTTC 3 cut(s) 1279, 1848, 2545
PcsI WCGNNNNNNNCGW 3 cut(s) 86, 2484, 2919
PctI GAATGC 2 cut(s) 91, 2601
PdmI GAANNNNTTC 2 cut(s) 1878, 2710
PfeI GAWTC 2 cut(s) 2015, 2639
PfoI TCCNGGA 3 cut(s) 841, 1326, 2856
PinAI ACCGGT 1 cut(s) 2204
PkrI GCNGC 8 cut(s) 136, 339, 352, 396, 922, 1663, 1828, 2829
PleI GAGTC 3 cut(s) 493, 910, 1809
PpsI GAGTC 3 cut(s) 493, 910, 1809
Ppu21I YACGTR 1 cut(s) 556
PpuMI RGGWCCY 6 cut(s) 543, 758, 1167, 1383, 2088, 2230
PshBI ATTAAT 2 cut(s) 1206, 1515
Psp124BI GAGCTC 1 cut(s) 1274
Psp5II RGGWCCY 6 cut(s) 543, 758, 1167, 1383, 2088, 2230
Psp6I CCWGG 6 cut(s) 176, 865, 936, 1326, 1821, 2856
PspEI GGTNACC 2 cut(s) 172, 362
PspFI CCCAGC 2 cut(s) 2307, 2728
PspGI CCWGG 6 cut(s) 176, 865, 936, 1326, 1821, 2856
PspPPI RGGWCCY 6 cut(s) 543, 758, 1167, 1383, 2088, 2230
PsrI GAACNNNNNNTAC 2 cut(s) 2680, 2712
PstI CTGCAG 1 cut(s) 1666
PstNI CAGNNNCTG 1 cut(s) 1994
PsuI RGATCY 2 cut(s) 995, 2839
PvuII CAGCTG 1 cut(s) 521
RseI CAYNNNNRTG 4 cut(s) 1055, 1835, 2519, 2871
SacI GAGCTC 1 cut(s) 1274
SalI GTCGAC 1 cut(s) 79
SapI GCTCTTC 3 cut(s) 1279, 1848, 2545
SatI GCNGC 8 cut(s) 135, 338, 351, 395, 921, 1662, 1827, 2828
ScaI AGTACT 1 cut(s) 604
SchI GAGTC 3 cut(s) 494, 911, 1810
ScrFI CCNGG 9 cut(s) 178, 317, 495, 843, 867, 938, 1328, 1823, 2858
SduI GDGCHC 3 cut(s) 95, 1274, 1472
SfcI CTRYAG 3 cut(s) 129, 516, 1662
SgrAI CRCCGGYG 1 cut(s) 2204
SinI GGWCC 8 cut(s) 157, 543, 758, 1167, 1383, 2077, 2088, 2230
SmiMI CAYNNNNRTG 4 cut(s) 1055, 1835, 2519, 2871
SmlI CTYRAG 2 cut(s) 1708, 2643
SmoI CTYRAG 2 cut(s) 1708, 2643
SnaBI TACGTA 1 cut(s) 556
SpeI ACTAGT 2 cut(s) 295, 1130
SphI GCATGC 1 cut(s) 1046
SsiI CCGC 8 cut(s) 381, 428, 1535, 1809, 1903, 2512, 2543, 2972
SspI AATATT 3 cut(s) 1204, 1210, 1311
SspMI CTAG 9 cut(s) 197, 296, 704, 762, 794, 906, 1131, 1161, 2021
SstI GAGCTC 1 cut(s) 1274
StyD4I CCNGG 9 cut(s) 176, 315, 493, 841, 865, 936, 1326, 1821, 2856
StyI CCWWGG 9 cut(s) 367, 547, 761, 1377, 1487, 1722, 1781, 2382, 2532
TaiI ACGT 5 cut(s) 558, 564, 1835, 1896, 2339
TaqII GACCGA 1 cut(s) 564
TatI WGTACW 2 cut(s) 602, 1575
TfiI GAWTC 2 cut(s) 2015, 2639
TseFI GTSAC 5 cut(s) 206, 941, 2402, 2659, 2918
TseI GCWGC 8 cut(s) 134, 337, 350, 394, 920, 1661, 1826, 2827
Tsp45I GTSAC 5 cut(s) 206, 941, 2402, 2659, 2918
TspDTI ATGAA 8 cut(s) 17, 1218, 1661, 1685, 1785, 2228, 2256, 2284
TspGWI ACGGA 2 cut(s) 1546, 1737
VpaK11BI GGWCC 8 cut(s) 157, 543, 758, 1167, 1383, 2077, 2088, 2230
VspI ATTAAT 2 cut(s) 1206, 1515
XapI RAATTY 2 cut(s) 636, 1018
XbaI TCTAGA 1 cut(s) 2020
XceI RCATGY 3 cut(s) 227, 1046, 2786
XmaJI CCTAGG 1 cut(s) 761
XmiI GTMKAC 1 cut(s) 80
XmnI GAANNNNTTC 2 cut(s) 1878, 2710
XspI CTAG 9 cut(s) 197, 296, 704, 762, 794, 906, 1131, 1161, 2021
ZrmI AGTACT 1 cut(s) 604
Zsp2I ATGCAT 1 cut(s) 1842
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.