MD03G1278700.v1.1

Belongs to the NPH3 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0005712
Physical Location & Seq
Reverse (-)
36055072 .. 36057343
2272 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1278700.v1.1.491

Sequence Viewer

Length: 423 bp
ATGGTAATGCCAATCTCTGATGTTTTTAGCGATCTTACTGTGGAAATAGGAGCATCAAGCTTTGCACTTCACAAGTTCCCCCTAGTTTCTAAGAGTGGAAGAATTCGGAGACTGTTGCTTGAAGCAAAAGATGCAAAACTTTCGCGCATAATTCTTGCTGCTGTTCCGGGTGGACCAGAGGGATTTGAGCTAGCTGCAAAGTTCTGTTACGGAGTTCATGTTGAAATTACACAGTCAAATGTTGCCATGCTTCGTTGCGTAGCGCACTTCCTGGAACTGACGGAAGAGTATGCTGAGAAAAATATGGAAACGAGAACTGAAGCTTATCTGAAGGAGACGGTGCTTCCAAACATATCAAAGTCGATATCAGTTCTTCGTCGCTATGAAACCTCTTGCCTGCAGCAGAAGAAACCAACCTTGTAA

Protein Analysis

141

Amino Acids

15.63

Weight (kDa)

8.45

Isoelectric Point (pI)

52.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
BTB PF00651 8 - 94 2.6e-06 BTB/POZ domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 145
AcsI RAATTY 1 cut(s) 102
AcuI CTGAAG 2 cut(s) 339, 350
AgsI TTSAA 2 cut(s) 122, 224
AjnI CCWGG 1 cut(s) 270
AluBI AGCT 4 cut(s) 60, 190, 194, 323
AluI AGCT 4 cut(s) 60, 190, 194, 323
Alw26I GTCTC 2 cut(s) 103, 329
ApeKI GCWGC 3 cut(s) 158, 194, 400
ApoI RAATTY 1 cut(s) 102
AspLEI GCGC 2 cut(s) 147, 265
AspS9I GGNCC 1 cut(s) 173
AsuC2I CCSGG 1 cut(s) 168
AsuNHI GCTAGC 1 cut(s) 190
AvaII GGWCC 1 cut(s) 173
BbvI GCAGC 3 cut(s) 145, 181, 412
BcgI CGANNNNNNTGC 2 cut(s) 123, 157
BciT130I CCWGG 1 cut(s) 272
BcnI CCSGG 1 cut(s) 168
BcoDI GTCTC 2 cut(s) 103, 329
BfaI CTAG 2 cut(s) 83, 191
BfmI CTRYAG 1 cut(s) 398
BisI GCNGC 3 cut(s) 159, 195, 401
BlsI GCNGC 3 cut(s) 160, 196, 402
Bme1390I CCNGG 2 cut(s) 168, 272
Bme18I GGWCC 1 cut(s) 173
BmgT120I GGNCC 1 cut(s) 173
BmrFI CCNGG 2 cut(s) 168, 272
BmsI GCATC 2 cut(s) 62, 121
BmtI GCTAGC 1 cut(s) 194
BpuMI CCSGG 1 cut(s) 168
BsaXI ACNNNNNCTCC 2 cut(s) 204, 234
BseBI CCWGG 1 cut(s) 272
BseMII CTCAG 1 cut(s) 285
BseXI GCAGC 3 cut(s) 145, 181, 412
Bsh1236I CGCG 1 cut(s) 145
BsiSI CCGG 1 cut(s) 167
BsmAI GTCTC 2 cut(s) 103, 329
BsmBI CGTCTC 1 cut(s) 329
Bsp143I GATC 1 cut(s) 31
BspCNI CTCAG 1 cut(s) 286
BspFNI CGCG 1 cut(s) 145
BspMAI CTGCAG 1 cut(s) 402
BspOI GCTAGC 1 cut(s) 194
BssMI GATC 1 cut(s) 31
Bst2UI CCWGG 1 cut(s) 272
Bst4CI ACNGT 4 cut(s) 40, 114, 234, 340
Bst6I CTCTTC 1 cut(s) 279
BstAPI GCANNNNNTGC 1 cut(s) 131
BstC8I GCNNGC 2 cut(s) 192, 398
BstDEI CTNAG 2 cut(s) 90, 294
BstFNI CGCG 1 cut(s) 145
BstHHI GCGC 2 cut(s) 147, 265
BstKTI GATC 1 cut(s) 34
BstMAI GTCTC 2 cut(s) 103, 329
BstMBI GATC 1 cut(s) 31
BstMWI GCNNNNNNNGC 1 cut(s) 131
BstNI CCWGG 1 cut(s) 272
BstSCI CCNGG 2 cut(s) 166, 270
BstSFI CTRYAG 1 cut(s) 398
BstUI CGCG 1 cut(s) 145
BstV1I GCAGC 3 cut(s) 145, 181, 412
Cac8I GCNNGC 2 cut(s) 192, 398
CfoI GCGC 2 cut(s) 147, 265
Cfr13I GGNCC 1 cut(s) 173
CviAII CATG 2 cut(s) 218, 247
CviJI RGCY 4 cut(s) 60, 190, 194, 323
CviKI_1 RGCY 4 cut(s) 60, 190, 194, 323
DdeI CTNAG 2 cut(s) 90, 294
DpnI GATC 1 cut(s) 33
DpnII GATC 1 cut(s) 31
Eam1104I CTCTTC 1 cut(s) 279
EarI CTCTTC 1 cut(s) 279
Eco32I GATATC 1 cut(s) 366
Eco47I GGWCC 1 cut(s) 173
Eco57I CTGAAG 2 cut(s) 339, 350
EcoRI GAATTC 1 cut(s) 102
EcoRII CCWGG 1 cut(s) 270
EcoRV GATATC 1 cut(s) 366
Esp3I CGTCTC 1 cut(s) 329
FaeI CATG 2 cut(s) 221, 250
FaiI YATR 7 cut(s) 149, 219, 248, 291, 305, 353, 384
FatI CATG 2 cut(s) 217, 246
Fnu4HI GCNGC 3 cut(s) 159, 195, 401
Fsp4HI GCNGC 3 cut(s) 159, 195, 401
FspBI CTAG 2 cut(s) 83, 191
GlaI GCGC 2 cut(s) 146, 264
GluI GCNGC 3 cut(s) 159, 195, 401
HapII CCGG 1 cut(s) 167
HhaI GCGC 2 cut(s) 147, 265
Hin1II CATG 2 cut(s) 221, 250
Hin6I GCGC 2 cut(s) 145, 263
HinP1I GCGC 2 cut(s) 145, 263
HindIII AAGCTT 2 cut(s) 58, 321
HpaII CCGG 1 cut(s) 167
Hpy166II GTNNAC 1 cut(s) 173
Hpy188I TCNGA 3 cut(s) 19, 108, 330
Hpy8I GTNNAC 1 cut(s) 173
Hpy99I CGWCG 1 cut(s) 381
HpyAV CCTTC 1 cut(s) 325
HpyCH4III ACNGT 4 cut(s) 40, 114, 234, 340
HpyCH4V TGCA 4 cut(s) 65, 134, 197, 400
HpyF10VI GCNNNNNNNGC 1 cut(s) 131
HpyF3I CTNAG 2 cut(s) 90, 294
Hsp92II CATG 2 cut(s) 221, 250
HspAI GCGC 2 cut(s) 145, 263
Kzo9I GATC 1 cut(s) 31
LmnI GCTCC 1 cut(s) 50
LpnPI CCDG 5 cut(s) 180, 189, 257, 284, 410
Lsp1109I GCAGC 3 cut(s) 145, 181, 412
LweI GCATC 2 cut(s) 62, 121
MaeI CTAG 2 cut(s) 83, 191
MaeIII GTNAC 1 cut(s) 206
MalI GATC 1 cut(s) 33
MboI GATC 1 cut(s) 31
MboII GAAGA 4 cut(s) 111, 296, 365, 418
MluCI AATT 3 cut(s) 102, 150, 225
MnlI CCTC 2 cut(s) 172, 400
MspI CCGG 1 cut(s) 167
MspR9I CCNGG 2 cut(s) 168, 272
MvaI CCWGG 1 cut(s) 272
MvnI CGCG 1 cut(s) 145
MwoI GCNNNNNNNGC 1 cut(s) 131
NciI CCSGG 1 cut(s) 168
NdeII GATC 1 cut(s) 31
NheI GCTAGC 1 cut(s) 190
NlaIII CATG 2 cut(s) 221, 250
PfoI TCCNGGA 1 cut(s) 270
PkrI GCNGC 3 cut(s) 160, 196, 402
Psp6I CCWGG 1 cut(s) 270
PspGI CCWGG 1 cut(s) 270
PspPI GGNCC 1 cut(s) 173
PstI CTGCAG 1 cut(s) 402
SatI GCNGC 3 cut(s) 159, 195, 401
Sau3AI GATC 1 cut(s) 31
Sau96I GGNCC 1 cut(s) 173
ScrFI CCNGG 2 cut(s) 168, 272
SetI ASST 6 cut(s) 62, 192, 196, 325, 392, 419
SfaNI GCATC 2 cut(s) 62, 121
SfcI CTRYAG 1 cut(s) 398
SinI GGWCC 1 cut(s) 173
Sse9I AATT 3 cut(s) 102, 150, 225
SspMI CTAG 2 cut(s) 83, 191
StyD4I CCNGG 2 cut(s) 166, 270
TaaI ACNGT 4 cut(s) 40, 114, 234, 340
TaqI TCGA 1 cut(s) 362
TasI AATT 3 cut(s) 102, 150, 225
TseI GCWGC 3 cut(s) 158, 194, 400
TspDTI ATGAA 2 cut(s) 206, 399
TspGWI ACGGA 2 cut(s) 225, 296
VpaK11BI GGWCC 1 cut(s) 173
XapI RAATTY 1 cut(s) 102
XspI CTAG 2 cut(s) 83, 191
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.