Rorug06G0231400

Belongs to the NPH3 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0005712
Physical Location & Seq
Forward (+)
37243417 .. 37248929
5513 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0231400.1

Sequence Viewer

Length: 1473 bp
ATGGCTGTTTCACAAAACAATCACGATTTGGAGGAGGGAACGCTAGAGGTCGGCATGGAGTACAGAACTGTCTCTGGTGTGGCTGGACCTCTGGTTATCCTTGAAAAAGTTAAGGGACCCAAGTTTCAGGAGATTGTTAATATTCGGTTGGGAGATGGAACAATTCGACGTGGCCAAGTCCTGGAGGTTGATGGAGAGAAAGCTATTGTTCAGGTTTTTGAAGGAACCTCTGGAATTGACAACAAGTATACCACAGTGCAGTTTACAGGAGAGGTGTTGAAAACTCCAGTCTCTCTGGACATGCTTGGGCGGATTTTTAATGGTTCTGGGAAACCCATTGACAATGGCCCCCCAATTTTGCCTGAGGCTTACCTAGACATATCTGGTAGTTCTATCAATCCCAGTGAGAGAACATATCCTGAAGAGATGATACAGACAGGAATTTCTACAATTGATGTTATGAATTCCATTGCCAGAGGACAAAAGATCCCACTTTTCTCTGCTGCTGGTCTACCTCATAATGAGATTGCAGCTCAGATATGTCGCCAGGCTGGTCTGGTCAAGCGGTTGGAGAAATCTGACAATCTTCTTGACGCTGGGGATGTGGAAGACGACAACTTCGCCATTGTGTTTGCTGCTATGGGAGTAAATATGGAGACCGCACAGTTCTTCAAACGTGATTTTGAGGAAAATGGTTCAATGGAGAGGGTCACCCTTTTTCTTAATCTGGCAAATGACCCTACAATTGAACGTATTATTACTCCTCGTATTGCTCTTACTACGGCAGAGTATTTGGCATATGAATGTGGGAAGCATGTCCTTGTCATACTGACAGATATGAGTTCTTATGCTGATGCTCTTCGTGAGGTATCTGCTGCTCGAGAGGAAGTGCCCGGAAGGCGTGGATATCCCGGGTACATGTATACTGATCTGGCACAAATCTATGAGCGTGCTGGAAGAATTGAAGGGCGAAAAGGCTCCATCACACAAATTCCAATTTTAACTATGCCCAATGATGATATTACACATCCCACCCCAGATCTTACGGGATATATCACTGAGGGACAGGTGTACATTGATAGGCAGCTATACAACAGACAGGTATACCCACCAATCAATGTCCTTCCATCACTTTCTCGTTTGATGAAGAGTGCTATTGGTGAAGGGATGACTCGCAAGGATCATTCTGATGTATCCAATCAGTTGTATGCAAATTATGCAATTGGGAAGGATGTCCAAGCAATGAAAGCTGTGGTTGGAGAAGAAGCTCTTTCTTCTGAGGACTTGCTATACCTGGAGTTTTTGGACAAGTTTGAGAAGAAATTTGTGAGCCAAGGAGCTTATGATACTCGTAGTATCTTCCAGTCTCTTGATTTGGCATGGACATTGCTACGCATTTTCCCTCGTGAGCTTCTCCACCGTATACCTTTAAAGACCCTAGATCTGTTCTACAGCAGAGATGCAACAAATTGA

Protein Analysis

490

Amino Acids

54.5

Weight (kDa)

4.94

Isoelectric Point (pI)

31.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ATP-synt_ab_N PF02874 24 - 90 8.4e-15 ATP synthase alpha/beta family, beta-barrel domain
ATP-synt_ab PF00006 147 - 379 1.1e-74 ATP synthase alpha/beta family, nucleotide-binding domain
ATP-synt_VA_C PF22919 385 - 483 9.7e-34 C-terminal domain of V and A type ATP synthase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 181
AccI GTMKAC 5 cut(s) 248, 511, 923, 1104, 1423
AciI CCGC 3 cut(s) 310, 565, 660
AclWI GGATC 2 cut(s) 481, 1188
AcoI YGGCCR 1 cut(s) 172
AcsI RAATTY 4 cut(s) 441, 463, 990, 1322
AcuI CTGAAG 1 cut(s) 441
AfaI GTAC 3 cut(s) 62, 917, 1073
AfiI CCNNNNNNNGG 1 cut(s) 181
AflIII ACRYGT 1 cut(s) 918
AgsI TTSAA 7 cut(s) 104, 221, 280, 673, 699, 749, 965
AjiI CACGTC 1 cut(s) 170
AjnI CCWGG 3 cut(s) 180, 546, 1293
AluBI AGCT 7 cut(s) 203, 533, 1087, 1250, 1268, 1340, 1411
AluI AGCT 7 cut(s) 203, 533, 1087, 1250, 1268, 1340, 1411
Alw26I GTCTC 4 cut(s) 76, 295, 650, 1371
AlwI GGATC 2 cut(s) 481, 1188
Ama87I CYCGRG 2 cut(s) 879, 911
AoxI GGCC 2 cut(s) 172, 346
ApeKI GCWGC 5 cut(s) 503, 530, 635, 875, 1084
ApoI RAATTY 4 cut(s) 441, 463, 990, 1322
ArsI GACNNNNNNTTYG 2 cut(s) 602, 634
AspS9I GGNCC 3 cut(s) 86, 116, 347
AsuC2I CCSGG 3 cut(s) 894, 912, 913
AsuHPI GGTGA 2 cut(s) 703, 1172
AvaI CYCGRG 2 cut(s) 879, 911
AvaII GGWCC 2 cut(s) 86, 116
AxyI CCTNAGG 1 cut(s) 363
BaeGI GKGCMC 1 cut(s) 894
BalI TGGCCA 1 cut(s) 174
BarI GAAGNNNNNNTAC 2 cut(s) 414, 446
BauI CACGAG 1 cut(s) 1404
BbsI GAAGAC 1 cut(s) 615
BbvI GCAGC 5 cut(s) 490, 542, 622, 862, 1096
BccI CCATC 4 cut(s) 149, 185, 989, 1135
BceAI ACGGC 1 cut(s) 798
BciT130I CCWGG 3 cut(s) 182, 548, 1295
BciVI GTATCC 1 cut(s) 1204
BcnI CCSGG 3 cut(s) 894, 912, 913
BcoDI GTCTC 4 cut(s) 76, 295, 650, 1371
BfaI CTAG 3 cut(s) 44, 374, 1439
BfmI CTRYAG 1 cut(s) 1450
BfuI GTATCC 1 cut(s) 1204
BglI GCCNNNNNGGC 1 cut(s) 898
BglII AGATCT 2 cut(s) 1039, 1441
BisI GCNGC 5 cut(s) 504, 531, 636, 876, 1085
BlsI GCNGC 5 cut(s) 505, 532, 637, 877, 1086
Bme1390I CCNGG 6 cut(s) 182, 548, 894, 912, 913, 1295
Bme18I GGWCC 2 cut(s) 86, 116
BmeT110I CYCGRG 2 cut(s) 879, 911
BmgBI CACGTC 1 cut(s) 170
BmgT120I GGNCC 3 cut(s) 86, 116, 347
BmiI GGNNCC 5 cut(s) 117, 118, 226, 349, 979
BmrFI CCNGG 6 cut(s) 182, 548, 894, 912, 913, 1295
BmrI ACTGGG 1 cut(s) 396
BmsI GCATC 2 cut(s) 844, 1450
BmuI ACTGGG 1 cut(s) 396
BpiI GAAGAC 1 cut(s) 615
BpmI CTGGAG 3 cut(s) 203, 270, 1316
BpuMI CCSGG 3 cut(s) 894, 912, 913
BsaI GGTCTC 1 cut(s) 650
BsaJI CCNNGG 2 cut(s) 911, 1333
Bsc4I CCNNNNNNNGG 1 cut(s) 181
Bse1I ACTGG 3 cut(s) 287, 402, 1363
Bse21I CCTNAGG 1 cut(s) 363
Bse3DI GCAATG 3 cut(s) 468, 1248, 1385
BseBI CCWGG 3 cut(s) 182, 548, 1295
BseDI CCNNGG 2 cut(s) 911, 1333
BseGI GGATG 4 cut(s) 607, 1027, 1173, 1237
BseLI CCNNNNNNNGG 1 cut(s) 181
BseMI GCAATG 3 cut(s) 468, 1248, 1385
BseMII CTCAG 4 cut(s) 354, 548, 1050, 1269
BseNI ACTGG 3 cut(s) 287, 402, 1363
BseRI GAGGAG 2 cut(s) 47, 753
BseSI GKGCMC 1 cut(s) 894
BseXI GCAGC 5 cut(s) 490, 542, 622, 862, 1096
BseYI CCCAGC 1 cut(s) 596
BsgI GTGCAG 1 cut(s) 278
BshFI GGCC 2 cut(s) 174, 348
BsiHKCI CYCGRG 2 cut(s) 879, 911
BsiSI CCGG 2 cut(s) 894, 912
BslFI GGGAC 2 cut(s) 129, 1077
BslI CCNNNNNNNGG 1 cut(s) 181
BsmAI GTCTC 4 cut(s) 76, 295, 650, 1371
BsmFI GGGAC 2 cut(s) 129, 1077
BsnI GGCC 2 cut(s) 174, 348
Bso31I GGTCTC 1 cut(s) 650
BsoBI CYCGRG 2 cut(s) 879, 911
Bsp1286I GDGCHC 1 cut(s) 894
Bsp1407I TGTACA 1 cut(s) 1071
Bsp143I GATC 5 cut(s) 486, 928, 1039, 1180, 1441
BspACI CCGC 3 cut(s) 310, 565, 660
BspANI GGCC 2 cut(s) 174, 348
BspCNI CTCAG 4 cut(s) 355, 547, 1051, 1270
BspLI GGNNCC 5 cut(s) 117, 118, 226, 349, 979
BspPI GGATC 2 cut(s) 481, 1188
BspQI GCTCTTC 1 cut(s) 864
BspTNI GGTCTC 1 cut(s) 650
BsrDI GCAATG 3 cut(s) 468, 1248, 1385
BsrGI TGTACA 1 cut(s) 1071
BsrI ACTGG 3 cut(s) 287, 402, 1363
BssECI CCNNGG 2 cut(s) 911, 1333
BssMI GATC 5 cut(s) 486, 928, 1039, 1180, 1441
BssNAI GTATAC 4 cut(s) 249, 924, 1105, 1424
BssSI CACGAG 1 cut(s) 1404
BssT1I CCWWGG 1 cut(s) 1333
Bst1107I GTATAC 4 cut(s) 249, 924, 1105, 1424
Bst2BI CACGAG 1 cut(s) 1404
Bst2UI CCWGG 3 cut(s) 182, 548, 1295
Bst4CI ACNGT 4 cut(s) 70, 256, 666, 1421
Bst6I CTCTTC 3 cut(s) 417, 864, 1142
BstAPI GCANNNNNTGC 1 cut(s) 1217
BstAUI TGTACA 1 cut(s) 1071
BstC8I GCNNGC 1 cut(s) 951
BstDEI CTNAG 4 cut(s) 363, 534, 1059, 1278
BstEII GGTNACC 1 cut(s) 709
BstF5I GGATG 4 cut(s) 607, 1027, 1173, 1237
BstKTI GATC 5 cut(s) 489, 931, 1042, 1183, 1444
BstMAI GTCTC 4 cut(s) 76, 295, 650, 1371
BstMBI GATC 5 cut(s) 486, 928, 1039, 1180, 1441
BstMWI GCNNNNNNNGC 3 cut(s) 898, 1217, 1247
BstNI CCWGG 3 cut(s) 182, 548, 1295
BstNSI RCATGY 3 cut(s) 304, 818, 922
BstPI GGTNACC 1 cut(s) 709
BstSCI CCNGG 6 cut(s) 180, 546, 892, 910, 911, 1293
BstSFI CTRYAG 1 cut(s) 1450
BstSLI GKGCMC 1 cut(s) 894
BstV1I GCAGC 5 cut(s) 490, 542, 622, 862, 1096
BstV2I GAAGAC 1 cut(s) 615
BstX2I RGATCY 3 cut(s) 486, 1039, 1441
BstYI RGATCY 3 cut(s) 486, 1039, 1441
BstZ17I GTATAC 4 cut(s) 249, 924, 1105, 1424
Bsu36I CCTNAGG 1 cut(s) 363
BsuI GTATCC 1 cut(s) 1204
BsuRI GGCC 2 cut(s) 174, 348
BtrI CACGTC 1 cut(s) 170
BtsCI GGATG 4 cut(s) 607, 1027, 1173, 1237
BtsIMutI CAGTG 3 cut(s) 261, 409, 1056
Cac8I GCNNGC 1 cut(s) 951
Cfr13I GGNCC 3 cut(s) 86, 116, 347
Cfr9I CCCGGG 1 cut(s) 911
CseI GACGC 1 cut(s) 602
Csp6I GTAC 3 cut(s) 61, 916, 1072
CviAII CATG 5 cut(s) 55, 301, 815, 919, 1380
CviQI GTAC 3 cut(s) 61, 916, 1072
DdeI CTNAG 4 cut(s) 363, 534, 1059, 1278
DpnI GATC 5 cut(s) 488, 930, 1041, 1182, 1443
DpnII GATC 5 cut(s) 486, 928, 1039, 1180, 1441
DraI TTTAAA 1 cut(s) 1431
EaeI YGGCCR 1 cut(s) 172
Eam1104I CTCTTC 3 cut(s) 417, 864, 1142
EarI CTCTTC 3 cut(s) 417, 864, 1142
EciI GGCGGA 1 cut(s) 325
Eco130I CCWWGG 1 cut(s) 1333
Eco31I GGTCTC 1 cut(s) 650
Eco32I GATATC 1 cut(s) 908
Eco47I GGWCC 2 cut(s) 86, 116
Eco57I CTGAAG 1 cut(s) 441
Eco81I CCTNAGG 1 cut(s) 363
Eco88I CYCGRG 2 cut(s) 879, 911
Eco91I GGTNACC 1 cut(s) 709
EcoO109I RGGNCCY 1 cut(s) 116
EcoO65I GGTNACC 1 cut(s) 709
EcoRI GAATTC 1 cut(s) 463
EcoRII CCWGG 3 cut(s) 180, 546, 1293
EcoRV GATATC 1 cut(s) 908
EcoT14I CCWWGG 1 cut(s) 1333
ErhI CCWWGG 1 cut(s) 1333
FaeI CATG 5 cut(s) 58, 304, 818, 922, 1383
FalI AAGNNNNNCTT 2 cut(s) 1254, 1286
FaqI GGGAC 2 cut(s) 129, 1077
FatI CATG 5 cut(s) 54, 300, 814, 918, 1379
FauNDI CATATG 1 cut(s) 799
FblI GTMKAC 5 cut(s) 248, 511, 923, 1104, 1423
Fnu4HI GCNGC 5 cut(s) 504, 531, 636, 876, 1085
FokI GGATG 4 cut(s) 614, 1014, 1180, 1244
Fsp4HI GCNGC 5 cut(s) 504, 531, 636, 876, 1085
FspBI CTAG 3 cut(s) 44, 374, 1439
GluI GCNGC 5 cut(s) 504, 531, 636, 876, 1085
GsaI CCCAGC 1 cut(s) 600
GsuI CTGGAG 3 cut(s) 203, 270, 1316
HaeIII GGCC 2 cut(s) 174, 348
HapII CCGG 2 cut(s) 894, 912
HgaI GACGC 1 cut(s) 602
Hin1II CATG 5 cut(s) 58, 304, 818, 922, 1383
HinfI GANTC 1 cut(s) 1171
HpaII CCGG 2 cut(s) 894, 912
HphI GGTGA 2 cut(s) 703, 1172
Hpy166II GTNNAC 7 cut(s) 249, 264, 512, 924, 1072, 1105, 1424
Hpy188I TCNGA 4 cut(s) 537, 580, 1189, 1279
Hpy8I GTNNAC 7 cut(s) 249, 264, 512, 924, 1072, 1105, 1424
Hpy99I CGWCG 1 cut(s) 171
HpyAV CCTTC 6 cut(s) 215, 891, 959, 1133, 1157, 1222
HpyCH4III ACNGT 4 cut(s) 70, 256, 666, 1421
HpyCH4IV ACGT 3 cut(s) 169, 676, 751
HpyCH4V TGCA 5 cut(s) 259, 530, 1211, 1220, 1463
HpyF10VI GCNNNNNNNGC 3 cut(s) 898, 1217, 1247
HpyF3I CTNAG 4 cut(s) 363, 534, 1059, 1278
HpySE526I ACGT 3 cut(s) 169, 676, 751
Hsp92II CATG 5 cut(s) 58, 304, 818, 922, 1383
KflI GGGWCCC 1 cut(s) 116
Kzo9I GATC 5 cut(s) 486, 928, 1039, 1180, 1441
LguI GCTCTTC 1 cut(s) 864
LmnI GCTCC 2 cut(s) 983, 1337
Lsp1109I GCAGC 5 cut(s) 490, 542, 622, 862, 1096
LweI GCATC 2 cut(s) 844, 1450
MaeI CTAG 3 cut(s) 44, 374, 1439
MaeII ACGT 3 cut(s) 169, 676, 751
MaeIII GTNAC 1 cut(s) 709
MalI GATC 5 cut(s) 488, 930, 1041, 1182, 1443
MboI GATC 5 cut(s) 486, 928, 1039, 1180, 1441
MfeI CAATTG 3 cut(s) 450, 744, 1221
MflI RGATCY 3 cut(s) 486, 1039, 1441
MhlI GDGCHC 1 cut(s) 894
MlsI TGGCCA 1 cut(s) 174
MluNI TGGCCA 1 cut(s) 174
MlyI GAGTC 1 cut(s) 1165
MmeI TCCRAC 2 cut(s) 549, 1237
Mox20I TGGCCA 1 cut(s) 174
MscI TGGCCA 1 cut(s) 174
MseI TTAA 6 cut(s) 111, 138, 318, 723, 1001, 1430
MslI CAYNNNNRTG 2 cut(s) 802, 1188
Msp20I TGGCCA 1 cut(s) 174
MspI CCGG 2 cut(s) 894, 912
MspR9I CCNGG 6 cut(s) 182, 548, 894, 912, 913, 1295
MunI CAATTG 3 cut(s) 450, 744, 1221
MvaI CCWGG 3 cut(s) 182, 548, 1295
MwoI GCNNNNNNNGC 3 cut(s) 898, 1217, 1247
NciI CCSGG 3 cut(s) 894, 912, 913
NdeI CATATG 1 cut(s) 799
NdeII GATC 5 cut(s) 486, 928, 1039, 1180, 1441
NlaIII CATG 5 cut(s) 58, 304, 818, 922, 1383
NlaIV GGNNCC 5 cut(s) 117, 118, 226, 349, 979
NmuCI GTSAC 1 cut(s) 709
NspI RCATGY 3 cut(s) 304, 818, 922
PaeR7I CTCGAG 1 cut(s) 879
PciI ACATGT 1 cut(s) 918
PciSI GCTCTTC 1 cut(s) 864
PflMI CCANNNNNTGG 1 cut(s) 181
PfoI TCCNGGA 1 cut(s) 180
PkrI GCNGC 5 cut(s) 505, 532, 637, 877, 1086
PleI GAGTC 1 cut(s) 1165
PpsI GAGTC 1 cut(s) 1165
PpuMI RGGWCCY 1 cut(s) 116
PscI ACATGT 1 cut(s) 918
Psp5II RGGWCCY 1 cut(s) 116
Psp6I CCWGG 3 cut(s) 180, 546, 1293
PspEI GGTNACC 1 cut(s) 709
PspFI CCCAGC 1 cut(s) 596
PspGI CCWGG 3 cut(s) 180, 546, 1293
PspN4I GGNNCC 5 cut(s) 117, 118, 226, 349, 979
PspPI GGNCC 3 cut(s) 86, 116, 347
PspPPI RGGWCCY 1 cut(s) 116
PsuI RGATCY 3 cut(s) 486, 1039, 1441
RsaI GTAC 3 cut(s) 62, 917, 1073
RsaNI GTAC 3 cut(s) 61, 916, 1072
RseI CAYNNNNRTG 2 cut(s) 802, 1188
SapI GCTCTTC 1 cut(s) 864
SaqAI TTAA 6 cut(s) 111, 138, 318, 723, 1001, 1430
SatI GCNGC 5 cut(s) 504, 531, 636, 876, 1085
Sau3AI GATC 5 cut(s) 486, 928, 1039, 1180, 1441
Sau96I GGNCC 3 cut(s) 86, 116, 347
SchI GAGTC 1 cut(s) 1165
ScrFI CCNGG 6 cut(s) 182, 548, 894, 912, 913, 1295
SduI GDGCHC 1 cut(s) 894
SfaNI GCATC 2 cut(s) 844, 1450
SfcI CTRYAG 1 cut(s) 1450
Sfr274I CTCGAG 1 cut(s) 879
SinI GGWCC 2 cut(s) 86, 116
SlaI CTCGAG 1 cut(s) 879
SmaI CCCGGG 1 cut(s) 913
SmiMI CAYNNNNRTG 2 cut(s) 802, 1188
SmlI CTYRAG 1 cut(s) 879
SmoI CTYRAG 1 cut(s) 879
SsiI CCGC 3 cut(s) 310, 565, 660
SspI AATATT 1 cut(s) 142
SspMI CTAG 3 cut(s) 44, 374, 1439
StyD4I CCNGG 6 cut(s) 180, 546, 892, 910, 911, 1293
StyI CCWWGG 1 cut(s) 1333
TaaI ACNGT 4 cut(s) 70, 256, 666, 1421
TaiI ACGT 3 cut(s) 172, 679, 754
TaqI TCGA 2 cut(s) 166, 880
TatI WGTACW 2 cut(s) 60, 1071
Tru1I TTAA 6 cut(s) 111, 138, 318, 723, 1001, 1430
Tru9I TTAA 6 cut(s) 111, 138, 318, 723, 1001, 1430
TscAI CASTG 3 cut(s) 261, 409, 1063
TseFI GTSAC 1 cut(s) 709
TseI GCWGC 5 cut(s) 503, 530, 635, 875, 1084
Tsp45I GTSAC 1 cut(s) 709
TspDTI ATGAA 4 cut(s) 476, 816, 1160, 1259
TspMI CCCGGG 1 cut(s) 911
TspRI CASTG 3 cut(s) 261, 409, 1063
Van91I CCANNNNNTGG 1 cut(s) 181
VpaK11BI GGWCC 2 cut(s) 86, 116
XapI RAATTY 4 cut(s) 441, 463, 990, 1322
XceI RCATGY 3 cut(s) 304, 818, 922
XhoI CTCGAG 1 cut(s) 879
XmaI CCCGGG 1 cut(s) 911
XmiI GTMKAC 5 cut(s) 248, 511, 923, 1104, 1423
XspI CTAG 3 cut(s) 44, 374, 1439
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.