Rh6DG344300

Belongs to the NPH3 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6D
OG0005712
Physical Location & Seq
Reverse (-)
54733836 .. 54737512
3677 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6DG344300.1

Sequence Viewer

Length: 1899 bp
ATGGGTGTTGTCACCGTTGGTGAATTGAAGCCTAGCATTTCAGGGAAGAGGTCTTTTCGTCCAAGCTCAAGTATAAGGCATGCCACAGAATGGCCAATCTCTGATGTTTCTAGTGATCTTACAGTGGAGATAGCAGCATCAAGCTTTGCCCTTCACAAGTTCCCTCTAGTTTCTCGGAGTGGAAAGATTCGGAAACTGTTGCTTGAAGCAAAAGATTCAAAAGTTTCACGTGTGATTCTTCCATCTGTTCCGGGCGGAGCAGAGGCATTTGAGCTAGCTGCAAAGTTCTGTTATGGCCATAATATTGAGATCACACAAGCAAATGTTGCCTTGCTCCGTTGCACAGCTCACTTTCTTGAAATGACGGAAGAGTATGCTGAGAAGAACCTGGAAACAAGAACTGAAGCTTATCTAAAGGATATGGTGTTCCCAAACATATCAAAATCAATAGCAGTTCTTCATCGTTGCGAAACACTCTTGCCTGCAGCAGAAGAAACCAACCTGGTTAGCAGGCTCATCAATGCAATCGCAAACAATGCGTGCAAAGAGCAGCTCACCTCTGGCTTACTGAAACTTGACCACAACTTTCCTTCAAAAGTAGCTACTATGGAACCTGAAACGCCCTCGGATTGGTGGGGAAAAGCGCTTTCGGTGCTTAGTCTTGACTTCTTCCAGAGGGTTTTATCTGCAGTGAAAACCAAGGGTCTTAAACAGGATATGATCAGCAAAATTTTGATAAATTACGCCCATAACTCTCTTCAAGGTCTTGTTGTAAGGGATGCGCAATTGGTCAAAGGAAGTCTCTTCGATTTAGAACTGCAAAAGAAACAAAGGGTCATTGTTGAAGCTATTGTTAGTTTACTACCAACACAATCAAGGAAGAGTCCAGTTCCAATGGCATTTCTTTCGAGTTTGTTGAAAACTGCAATTGCAGCGTCAGCATCCACTTCATGCAGATCTGATTTGGAGAGGCGGATTGGTTTGCAGCTGGATCAGGCAATTCTTGAAGACATCCTCATTCCTACAAACTCACATGGAAACAACCACAGCACAATCTATGATGCCGACACTATAGTGAGAATCTTCTCCATATTTTTGAACTTGGATGAGGATGATGAAGATGATACTAATCTGTTAAGAGATGAAAGTCAAATGGTTTATGATTTCGACAGCCCTGGATCTCCTAAACAAAGTTCAATCTTGAAGGTCTCTAAGTTACTGGACAATTATCTTGCCGAAGTTGCATTGGACCCAAACTTGTCACCATCAAAGTTCACAGCTCTAGCAGAACTACTTCCAGACCATGCACGAATAGTAAGTGATGGACTCTACAGAGCTGTGGATATCTTCCTCAAAGTTCACCCAAACATGAAGGACTCTGAAAGGTACCGGCTTTGCAAAACCATAGATTGCCAGAAACTATCCCAAGAAGCCTGCAGTCATGCAGCACAGAATGAAAGGCTACCGGTACAGATGGCAGTTCAGGTCCTATACTTTGAGCAAATCAGGCTGAGGAATGCAATGAATGGTGGCCACAATCAGTTCTTCTTTGGCGCAATTAATGGCACTCAATTCCCTCAAAGATCAGGCAGTGGTGCTGGCAGTGGAGCCATTTCCCCTAGAGACAACTATGCATCAGTTCGGAGAGAGAATCGAGAGCTGAAGCTTGAAGTCGCTAGAATGAGAATGAGGCTGACAGACTTGGAAAAAGATCATGTCTCTATGAAGCAAGAGCTTGTTAAGACACACCCTGCAAATAAGTTGTTCAAATCCTTCACCAAAAAATTGGGGAAGATCAATGCATTGTTCCGGATTAATAGTATCAAGACCTTGGGGGGTAAGGCGCATTCGGAGAGCAGATTCCCGTTTCAGAAGCGAAGGCGCCATTCAGTTTCATGA

Protein Analysis

632

Amino Acids

70.51

Weight (kDa)

9.12

Isoelectric Point (pI)

45.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NPH3 PF03000 210 - 479 5.3e-90 NPH3 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 783
Acc65I GGTACC 1 cut(s) 1386
AccB1I GGYRCC 2 cut(s) 1386, 1881
AccB7I CCANNNNNTGG 1 cut(s) 90
AccIII TCCGGA 1 cut(s) 1809
AciI CCGC 2 cut(s) 255, 973
AclWI GGATC 2 cut(s) 999, 1186
AcoI YGGCCR 3 cut(s) 92, 295, 1531
AcsI RAATTY 1 cut(s) 729
AcuI CTGAAG 2 cut(s) 423, 1682
AcvI CACGTG 1 cut(s) 230
AcyI GRCGYC 1 cut(s) 1882
AfaI GTAC 2 cut(s) 1388, 1470
AfeI AGCGCT 1 cut(s) 645
AfiI CCNNNNNNNGG 2 cut(s) 90, 630
AflIII ACRYGT 1 cut(s) 229
AgeI ACCGGT 1 cut(s) 1465
AjnI CCWGG 3 cut(s) 387, 501, 1174
AleI CACNNNNGTG 1 cut(s) 1073
AloI GAACNNNNNNTCC 2 cut(s) 410, 442
Alw26I GTCTC 4 cut(s) 806, 1213, 1617, 1723
AlwI GGATC 2 cut(s) 999, 1186
Aor13HI TCCGGA 1 cut(s) 1809
Aor51HI AGCGCT 1 cut(s) 645
AoxI GGCC 3 cut(s) 92, 295, 1531
ApeKI GCWGC 7 cut(s) 134, 278, 485, 550, 932, 985, 1445
ApoI RAATTY 1 cut(s) 729
AseI ATTAAT 2 cut(s) 1560, 1815
AsiGI ACCGGT 1 cut(s) 1465
Asp700I GAANNNNTTC 1 cut(s) 1293
Asp718I GGTACC 1 cut(s) 1386
AspLEI GCGC 5 cut(s) 646, 784, 1556, 1846, 1884
AspS9I GGNCC 2 cut(s) 1249, 1486
AsuC2I CCSGG 1 cut(s) 252
AsuHPI GGTGA 6 cut(s) 4, 32, 547, 1254, 1352, 1768
AsuNHI GCTAGC 1 cut(s) 274
AvaII GGWCC 2 cut(s) 1249, 1486
BalI TGGCCA 3 cut(s) 94, 297, 1533
BanI GGYRCC 2 cut(s) 1386, 1881
BbrPI CACGTG 1 cut(s) 230
BbsI GAAGAC 1 cut(s) 1014
BbvCI CCTCAGC 1 cut(s) 1511
BbvI GCAGC 7 cut(s) 146, 265, 497, 562, 944, 997, 1457
BccI CCATC 4 cut(s) 250, 1273, 1316, 1468
BcgI CGANNNNNNTGC 2 cut(s) 888, 922
BciT130I CCWGG 3 cut(s) 389, 503, 1176
BclI TGATCA 1 cut(s) 720
BcnI CCSGG 1 cut(s) 252
BcoDI GTCTC 4 cut(s) 806, 1213, 1617, 1723
BfaI CTAG 7 cut(s) 33, 111, 167, 275, 1283, 1620, 1677
BfmI CTRYAG 5 cut(s) 483, 687, 1071, 1330, 1435
BfoI RGCGCY 2 cut(s) 647, 1885
BglII AGATCT 1 cut(s) 956
BisI GCNGC 7 cut(s) 135, 279, 486, 551, 933, 986, 1446
BlsI GCNGC 7 cut(s) 136, 280, 487, 552, 934, 987, 1447
Bme1390I CCNGG 4 cut(s) 252, 389, 503, 1176
Bme18I GGWCC 2 cut(s) 1249, 1486
BmgT120I GGNCC 2 cut(s) 1249, 1486
BmiI GGNNCC 5 cut(s) 612, 1251, 1388, 1609, 1883
BmrFI CCNGG 4 cut(s) 252, 389, 503, 1176
BmsI GCATC 5 cut(s) 146, 769, 950, 1051, 1643
BmtI GCTAGC 1 cut(s) 278
BpiI GAAGAC 1 cut(s) 1014
Bpu10I CCTNAGC 1 cut(s) 1511
BpuEI CTTGAG 1 cut(s) 52
BpuMI CCSGG 1 cut(s) 252
BsaAI YACGTR 1 cut(s) 230
BsaBI GATNNNNATC 1 cut(s) 1128
BsaHI GRCGYC 1 cut(s) 1882
BsaI GGTCTC 1 cut(s) 1213
BsaJI CCNNGG 4 cut(s) 624, 699, 1174, 1830
BsaWI WCCGGW 2 cut(s) 1465, 1809
Bsc4I CCNNNNNNNGG 2 cut(s) 90, 630
Bse118I RCCGGY 2 cut(s) 1389, 1465
Bse1I ACTGG 2 cut(s) 887, 1224
Bse3DI GCAATG 1 cut(s) 1527
Bse8I GATNNNNATC 1 cut(s) 1128
BseAI TCCGGA 1 cut(s) 1809
BseBI CCWGG 3 cut(s) 389, 503, 1176
BseDI CCNNGG 4 cut(s) 624, 699, 1174, 1830
BseGI GGATG 5 cut(s) 784, 941, 1011, 1111, 1117
BseJI GATNNNNATC 1 cut(s) 1128
BseLI CCNNNNNNNGG 2 cut(s) 90, 630
BseMI GCAATG 1 cut(s) 1527
BseMII CTCAG 2 cut(s) 369, 1502
BseNI ACTGG 2 cut(s) 887, 1224
BseXI GCAGC 7 cut(s) 146, 265, 497, 562, 944, 997, 1457
BshFI GGCC 3 cut(s) 94, 297, 1533
BshNI GGYRCC 2 cut(s) 1386, 1881
BshTI ACCGGT 1 cut(s) 1465
BsiSI CCGG 4 cut(s) 251, 1390, 1466, 1810
BslI CCNNNNNNNGG 2 cut(s) 90, 630
BsmAI GTCTC 4 cut(s) 806, 1213, 1617, 1723
BsmI GAATGC 2 cut(s) 1522, 1846
BsnI GGCC 3 cut(s) 94, 297, 1533
Bso31I GGTCTC 1 cut(s) 1213
Bsp13I TCCGGA 1 cut(s) 1809
Bsp143I GATC 9 cut(s) 115, 309, 720, 956, 991, 1178, 1583, 1711, 1794
BspACI CCGC 2 cut(s) 255, 973
BspANI GGCC 3 cut(s) 94, 297, 1533
BspCNI CTCAG 2 cut(s) 370, 1503
BspEI TCCGGA 1 cut(s) 1809
BspHI TCATGA 1 cut(s) 1895
BspLI GGNNCC 5 cut(s) 612, 1251, 1388, 1609, 1883
BspMAI CTGCAG 3 cut(s) 487, 691, 1439
BspOI GCTAGC 1 cut(s) 278
BspPI GGATC 2 cut(s) 999, 1186
BspT107I GGYRCC 2 cut(s) 1386, 1881
BspTNI GGTCTC 1 cut(s) 1213
BsrDI GCAATG 1 cut(s) 1527
BsrFI RCCGGY 2 cut(s) 1389, 1465
BsrI ACTGG 2 cut(s) 887, 1224
BssAI RCCGGY 2 cut(s) 1389, 1465
BssECI CCNNGG 4 cut(s) 624, 699, 1174, 1830
BssMI GATC 9 cut(s) 115, 309, 720, 956, 991, 1178, 1583, 1711, 1794
BssNI GRCGYC 1 cut(s) 1882
BssT1I CCWWGG 2 cut(s) 699, 1830
Bst2UI CCWGG 3 cut(s) 389, 503, 1176
Bst4CI ACNGT 3 cut(s) 16, 124, 198
Bst6I CTCTTC 5 cut(s) 41, 363, 762, 809, 875
BstACI GRCGYC 1 cut(s) 1882
BstAPI GCANNNNNTGC 2 cut(s) 326, 536
BstBAI YACGTR 1 cut(s) 230
BstC8I GCNNGC 7 cut(s) 81, 276, 483, 512, 541, 1435, 1600
BstDEI CTNAG 4 cut(s) 378, 656, 1212, 1511
BstF5I GGATG 5 cut(s) 784, 941, 1011, 1111, 1117
BstH2I RGCGCY 2 cut(s) 647, 1885
BstHHI GCGC 5 cut(s) 646, 784, 1556, 1846, 1884
BstKTI GATC 9 cut(s) 118, 312, 723, 959, 994, 1181, 1586, 1714, 1797
BstMAI GTCTC 4 cut(s) 806, 1213, 1617, 1723
BstMBI GATC 9 cut(s) 115, 309, 720, 956, 991, 1178, 1583, 1711, 1794
BstMWI GCNNNNNNNGC 7 cut(s) 326, 536, 652, 932, 938, 1241, 1507
BstNI CCWGG 3 cut(s) 389, 503, 1176
BstNSI RCATGY 1 cut(s) 83
BstSCI CCNGG 4 cut(s) 250, 387, 501, 1174
BstSFI CTRYAG 5 cut(s) 483, 687, 1071, 1330, 1435
BstV1I GCAGC 7 cut(s) 146, 265, 497, 562, 944, 997, 1457
BstV2I GAAGAC 1 cut(s) 1014
BstX2I RGATCY 2 cut(s) 956, 1178
BstXI CCANNNNNNTGG 1 cut(s) 1786
BstYI RGATCY 2 cut(s) 956, 1178
BsuRI GGCC 3 cut(s) 94, 297, 1533
BtsCI GGATG 5 cut(s) 784, 941, 1011, 1111, 1117
BtsI GCAGTG 3 cut(s) 696, 1597, 1609
BtsIMutI CAGTG 4 cut(s) 129, 696, 1597, 1609
Cac8I GCNNGC 7 cut(s) 81, 276, 483, 512, 541, 1435, 1600
CciI TCATGA 1 cut(s) 1895
CfoI GCGC 5 cut(s) 646, 784, 1556, 1846, 1884
Cfr10I RCCGGY 2 cut(s) 1389, 1465
Cfr13I GGNCC 2 cut(s) 1249, 1486
CseI GACGC 1 cut(s) 924
CsiI ACCWGGT 1 cut(s) 501
Csp6I GTAC 2 cut(s) 1387, 1469
CspAI ACCGGT 1 cut(s) 1465
CviAII CATG 8 cut(s) 80, 951, 1034, 1304, 1369, 1442, 1715, 1896
CviQI GTAC 2 cut(s) 1387, 1469
DdeI CTNAG 4 cut(s) 378, 656, 1212, 1511
DinI GGCGCC 1 cut(s) 1883
DpnI GATC 9 cut(s) 117, 311, 722, 958, 993, 1180, 1585, 1713, 1796
DpnII GATC 9 cut(s) 115, 309, 720, 956, 991, 1178, 1583, 1711, 1794
EaeI YGGCCR 3 cut(s) 92, 295, 1531
Eam1104I CTCTTC 5 cut(s) 41, 363, 762, 809, 875
EarI CTCTTC 5 cut(s) 41, 363, 762, 809, 875
EciI GGCGGA 2 cut(s) 270, 988
Eco130I CCWWGG 2 cut(s) 699, 1830
Eco31I GGTCTC 1 cut(s) 1213
Eco32I GATATC 1 cut(s) 1345
Eco47I GGWCC 2 cut(s) 1249, 1486
Eco47III AGCGCT 1 cut(s) 645
Eco57I CTGAAG 2 cut(s) 423, 1682
Eco72I CACGTG 1 cut(s) 230
EcoO109I RGGNCCY 1 cut(s) 1486
EcoRII CCWGG 3 cut(s) 387, 501, 1174
EcoRV GATATC 1 cut(s) 1345
EcoT14I CCWWGG 2 cut(s) 699, 1830
EcoT22I ATGCAT 2 cut(s) 1636, 1804
EgeI GGCGCC 1 cut(s) 1883
EheI GGCGCC 1 cut(s) 1883
ErhI CCWWGG 2 cut(s) 699, 1830
FaeI CATG 8 cut(s) 83, 954, 1037, 1307, 1372, 1445, 1718, 1899
FatI CATG 8 cut(s) 79, 950, 1033, 1303, 1368, 1441, 1714, 1895
FbaI TGATCA 1 cut(s) 720
Fnu4HI GCNGC 7 cut(s) 135, 279, 486, 551, 933, 986, 1446
FokI GGATG 5 cut(s) 791, 928, 998, 1118, 1124
Fsp4HI GCNGC 7 cut(s) 135, 279, 486, 551, 933, 986, 1446
FspBI CTAG 7 cut(s) 33, 111, 167, 275, 1283, 1620, 1677
FspI TGCGCA 1 cut(s) 783
GlaI GCGC 5 cut(s) 645, 783, 1555, 1845, 1883
GluI GCNGC 7 cut(s) 135, 279, 486, 551, 933, 986, 1446
HaeII RGCGCY 2 cut(s) 647, 1885
HaeIII GGCC 3 cut(s) 94, 297, 1533
HapII CCGG 4 cut(s) 251, 1390, 1466, 1810
HgaI GACGC 1 cut(s) 924
HhaI GCGC 5 cut(s) 646, 784, 1556, 1846, 1884
Hin1I GRCGYC 1 cut(s) 1882
Hin1II CATG 8 cut(s) 83, 954, 1037, 1307, 1372, 1445, 1718, 1899
Hin6I GCGC 5 cut(s) 644, 782, 1554, 1844, 1882
HinP1I GCGC 5 cut(s) 644, 782, 1554, 1844, 1882
HindIII AAGCTT 3 cut(s) 142, 405, 1664
HinfI GANTC 9 cut(s) 187, 215, 235, 883, 1080, 1326, 1376, 1651, 1860
HpaII CCGG 4 cut(s) 251, 1390, 1466, 1810
HphI GGTGA 6 cut(s) 4, 32, 547, 1254, 1352, 1768
Hpy166II GTNNAC 3 cut(s) 860, 1275, 1360
Hpy188I TCNGA 9 cut(s) 103, 177, 192, 628, 961, 1381, 1644, 1852, 1872
Hpy8I GTNNAC 3 cut(s) 860, 1275, 1360
HpyAV CCTTC 6 cut(s) 161, 600, 1198, 1366, 1783, 1872
HpyCH4III ACNGT 3 cut(s) 16, 124, 198
HpyCH4IV ACGT 1 cut(s) 229
HpyF10VI GCNNNNNNNGC 7 cut(s) 326, 536, 652, 932, 938, 1241, 1507
HpyF3I CTNAG 4 cut(s) 378, 656, 1212, 1511
HpySE526I ACGT 1 cut(s) 229
Hsp92I GRCGYC 1 cut(s) 1882
Hsp92II CATG 8 cut(s) 83, 954, 1037, 1307, 1372, 1445, 1718, 1899
HspAI GCGC 5 cut(s) 644, 782, 1554, 1844, 1882
KasI GGCGCC 1 cut(s) 1881
Kpn2I TCCGGA 1 cut(s) 1809
KpnI GGTACC 1 cut(s) 1390
Ksp22I TGATCA 1 cut(s) 720
Kzo9I GATC 9 cut(s) 115, 309, 720, 956, 991, 1178, 1583, 1711, 1794
LmnI GCTCC 3 cut(s) 257, 339, 1607
Lsp1109I GCAGC 7 cut(s) 146, 265, 497, 562, 944, 997, 1457
LweI GCATC 5 cut(s) 146, 769, 950, 1051, 1643
MabI ACCWGGT 1 cut(s) 501
MaeI CTAG 7 cut(s) 33, 111, 167, 275, 1283, 1620, 1677
MaeII ACGT 1 cut(s) 229
MaeIII GTNAC 3 cut(s) 10, 1215, 1260
MalI GATC 9 cut(s) 117, 311, 722, 958, 993, 1180, 1585, 1713, 1796
MboI GATC 9 cut(s) 115, 309, 720, 956, 991, 1178, 1583, 1711, 1794
MfeI CAATTG 2 cut(s) 785, 927
MflI RGATCY 2 cut(s) 956, 1178
MlsI TGGCCA 3 cut(s) 94, 297, 1533
MluNI TGGCCA 3 cut(s) 94, 297, 1533
Mly113I GGCGCC 1 cut(s) 1882
MlyI GAGTC 3 cut(s) 892, 1320, 1370
Mox20I TGGCCA 3 cut(s) 94, 297, 1533
Mph1103I ATGCAT 2 cut(s) 1636, 1804
MroI TCCGGA 1 cut(s) 1809
MroXI GAANNNNTTC 1 cut(s) 1293
MscI TGGCCA 3 cut(s) 94, 297, 1533
MseI TTAA 5 cut(s) 708, 1136, 1560, 1740, 1815
MslI CAYNNNNRTG 1 cut(s) 1073
Msp20I TGGCCA 3 cut(s) 94, 297, 1533
MspA1I CMGCKG 1 cut(s) 988
MspI CCGG 4 cut(s) 251, 1390, 1466, 1810
MspR9I CCNGG 4 cut(s) 252, 389, 503, 1176
MunI CAATTG 2 cut(s) 785, 927
Mva1269I GAATGC 2 cut(s) 1522, 1846
MvaI CCWGG 3 cut(s) 389, 503, 1176
MwoI GCNNNNNNNGC 7 cut(s) 326, 536, 652, 932, 938, 1241, 1507
NarI GGCGCC 1 cut(s) 1882
NciI CCSGG 1 cut(s) 252
NdeII GATC 9 cut(s) 115, 309, 720, 956, 991, 1178, 1583, 1711, 1794
NheI GCTAGC 1 cut(s) 274
NlaIII CATG 8 cut(s) 83, 954, 1037, 1307, 1372, 1445, 1718, 1899
NlaIV GGNNCC 5 cut(s) 612, 1251, 1388, 1609, 1883
NmuCI GTSAC 2 cut(s) 10, 1260
NsbI TGCGCA 1 cut(s) 783
NsiI ATGCAT 2 cut(s) 1636, 1804
NspI RCATGY 1 cut(s) 83
OliI CACNNNNGTG 1 cut(s) 1073
PaeI GCATGC 1 cut(s) 83
PagI TCATGA 1 cut(s) 1895
PctI GAATGC 2 cut(s) 1522, 1846
PdmI GAANNNNTTC 1 cut(s) 1293
PfeI GAWTC 6 cut(s) 187, 215, 235, 1080, 1651, 1860
PflMI CCANNNNNTGG 1 cut(s) 90
PinAI ACCGGT 1 cut(s) 1465
PkrI GCNGC 7 cut(s) 136, 280, 487, 552, 934, 987, 1447
PleI GAGTC 3 cut(s) 891, 1320, 1370
PluTI GGCGCC 1 cut(s) 1885
PmaCI CACGTG 1 cut(s) 230
PmlI CACGTG 1 cut(s) 230
PpsI GAGTC 3 cut(s) 891, 1320, 1370
Ppu21I YACGTR 1 cut(s) 230
PpuMI RGGWCCY 1 cut(s) 1486
PshBI ATTAAT 2 cut(s) 1560, 1815
Psp5II RGGWCCY 1 cut(s) 1486
Psp6I CCWGG 3 cut(s) 387, 501, 1174
PspCI CACGTG 1 cut(s) 230
PspGI CCWGG 3 cut(s) 387, 501, 1174
PspN4I GGNNCC 5 cut(s) 612, 1251, 1388, 1609, 1883
PspPI GGNCC 2 cut(s) 1249, 1486
PspPPI RGGWCCY 1 cut(s) 1486
PstI CTGCAG 3 cut(s) 487, 691, 1439
PsuI RGATCY 2 cut(s) 956, 1178
PvuII CAGCTG 1 cut(s) 988
RsaI GTAC 2 cut(s) 1388, 1470
RsaNI GTAC 2 cut(s) 1387, 1469
RseI CAYNNNNRTG 1 cut(s) 1073
SaqAI TTAA 5 cut(s) 708, 1136, 1560, 1740, 1815
SatI GCNGC 7 cut(s) 135, 279, 486, 551, 933, 986, 1446
Sau3AI GATC 9 cut(s) 115, 309, 720, 956, 991, 1178, 1583, 1711, 1794
Sau96I GGNCC 2 cut(s) 1249, 1486
SchI GAGTC 3 cut(s) 892, 1320, 1370
ScrFI CCNGG 4 cut(s) 252, 389, 503, 1176
SexAI ACCWGGT 1 cut(s) 501
SfaNI GCATC 5 cut(s) 146, 769, 950, 1051, 1643
SfcI CTRYAG 5 cut(s) 483, 687, 1071, 1330, 1435
SfoI GGCGCC 1 cut(s) 1883
SinI GGWCC 2 cut(s) 1249, 1486
SmiMI CAYNNNNRTG 1 cut(s) 1073
SmlI CTYRAG 1 cut(s) 67
SmoI CTYRAG 1 cut(s) 67
SphI GCATGC 1 cut(s) 83
SsiI CCGC 2 cut(s) 255, 973
SspDI GGCGCC 1 cut(s) 1881
SspI AATATT 1 cut(s) 304
SspMI CTAG 7 cut(s) 33, 111, 167, 275, 1283, 1620, 1677
StyD4I CCNGG 4 cut(s) 250, 387, 501, 1174
StyI CCWWGG 2 cut(s) 699, 1830
TaaI ACNGT 3 cut(s) 16, 124, 198
TaiI ACGT 1 cut(s) 232
TaqI TCGA 4 cut(s) 807, 908, 1167, 1654
TfiI GAWTC 6 cut(s) 187, 215, 235, 1080, 1651, 1860
Tru1I TTAA 5 cut(s) 708, 1136, 1560, 1740, 1815
Tru9I TTAA 5 cut(s) 708, 1136, 1560, 1740, 1815
TscAI CASTG 4 cut(s) 129, 696, 1597, 1609
TseFI GTSAC 2 cut(s) 10, 1260
TseI GCWGC 7 cut(s) 134, 278, 485, 550, 932, 985, 1445
Tsp45I GTSAC 2 cut(s) 10, 1260
TspDTI ATGAA 9 cut(s) 449, 939, 1131, 1158, 1385, 1470, 1538, 1739, 1884
TspGWI ACGGA 2 cut(s) 326, 380
TspRI CASTG 4 cut(s) 129, 696, 1597, 1609
Van91I CCANNNNNTGG 1 cut(s) 90
VpaK11BI GGWCC 2 cut(s) 1249, 1486
VspI ATTAAT 2 cut(s) 1560, 1815
XapI RAATTY 1 cut(s) 729
XceI RCATGY 1 cut(s) 83
XmnI GAANNNNTTC 1 cut(s) 1293
XspI CTAG 7 cut(s) 33, 111, 167, 275, 1283, 1620, 1677
Zsp2I ATGCAT 2 cut(s) 1636, 1804
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.