Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr03

Orthogroup ID

OG0003006

Physical Location & Seq

Genomic Coordinates Reverse (-)

36446765 .. 36450793

Gene Structure 4029 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD03G1284200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1467 bp

ATGGGATTCAGATTCCACAATCTCAATTCTGTACGCAAAAGGCTCGCTTTCCACGTTCACAAGCACAATCATCGTCCTCCTCTTCCCAGTCCCACAATATCCCAGCCCCTCGATTCCCATCTGCAAAACCCTATTTACTCGATCGCCAGACGGTGGCATTTGGGCCACTCCCACCACCAAAACGATGATCAGCATCGCCTCTCCGGTGAAGAGGGGGAGAAGATTTTCCGGATGGGATTGGCAGCTGATGTTGGACTGGCTGCCGGGAAAGCTTTGACGGGTTACTTGTCCGGAAGCACGGCGATCATTGCTGATGCCGCCCATTCGGTCTCCGACGTGGTTCTTAGCAGCGTTGCTTTATGGTCATTTAAAGCTGCAAAGGCTCCGAAAGACAAAGAACACCCATATGGACACGGTAAATTTGAGACTCTTGGAGCACTTGGAATCTCTTGTATGCTGTTGGCCACGGCTGGTGGCATTGCATGGCATGCTTTGGATCTTTTGATGGGATTGTTTTCAGCAGATCCTGCTATAGTTAGTCAGTCATTGACACATGAGCATGGGCATAGTCATCGTCATCATGGAGTTGACATGGAGTACCCTGTTCTAGCACTGAGCATGACTGTTTTTTCAATATCTGTTAAGGAAGGACTTTACTGGATTACATTACGGGCTGGGGAAAGACAAGGTAGTGGACTGATGAAAGCAAATGCATGGCATCATCGTGCAGATGCCATTTCGTCAGTAGTTGCTCTCATTGGAGTTGGAGGTTCAATCCTTGGAGTAAAGTTTCTGGATCCCCTAGCTGGACTTCTTGTCGCAGGCATGATCCTGAAGGCGGGAATTGAAACTGGGCATCAGAGTGTCTTGGAACTGGTGGATGCTGCAATCCCAGCAGAACTTTTGGATCCTATGAAACAAACAATTGTACAAGTCGAGGGTGTGAAGGGTTGTCATCGCTTGAGGGCAAGGAGGGCTGGTTCATCTCTATACCTTGATGTACATATTGAGGTTGATCCTTTTTGTAGTGTTAGCGCTGCACATGACATTGGAGAAAATGTTCGTCGTCAGATTCACAAATCCCATCCTGAAGTCTCTGAGGTTTTCATACACATAGATCCTTCAATTTCACAAATTTGCCCCAGTGAAGATCAGCCAGAAATTCTCAAAGAAACTGCATGCCCAAATGTTTCTCCTGCAGAAAAAGACATTGAGGCAGCTGTTTCTACCATCATCTCGTCGAAGTATGCAGAGAAAATGGTTGTTGAACGCATAACGCATCACTTGTTACAAGGCAAGATGTATCTCCAAATTGAGGTTTCCATGCCCCCGGACATTGTAATTCGAGATGCAATGGAGGTGGCAAAAGAAGCCGAGCAAGACATTTTGACGGCGGCGACGAATCTCATTCACGTCGGCATTCAGCTGCGTCTGAGGAATCCAATTCCACAGTTCAACAGTGACTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Cation_efflux ZT_dimer

Protein Analysis

489

Amino Acids

53.18

Weight (kDa)

6.48

Isoelectric Point (pI)

47.38

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Cation_efflux	PF01545	79 - 293	3.1e-40	Cation efflux transmembrane domain
ZT_dimer	PF16916	298 - 374	1.7e-22	Cation efflux protein, cytoplasmic domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003006)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G47830 AT2G47830
fragaria_vesca	FvH4_c6g00080 FvH4_c6g00080 FvH4_c6g00080 FvH4_c6g00080 FvH4_c6g00080 FvH4_c6g00080 FvH4_c6g00080 FvH4_c6g00080 FvH4_c6g00110 FvH4_c6g00110 FvH4_c6g00110 FvH4_c6g00110 FvH4_c6g00110 FvH4_c6g00110 FvH4_c6g00110
malus_domestica	MD03G1284200.v1.1
prunus_persica	Prupe.8G255700_v2.0.a1 Prupe.8G255700_v2.0.a1 Prupe.8G255700_v2.0.a1 Prupe.8G255700_v2.0.a1
pyrus_communis	pycom03g22500
rosa_chinensis	RchiOBHm_Chr6g0293181
rosa_laevigata	RLG00000011969
rosa_multiflora	Rmu_co8409675.1_g000001 Rmu_sc0014786.1_g000003 Rmu_sc0039259.1_g000001 Rmu_sc0042811.1_g000001 Rmu_sc0042818.1_g000001
rosa_roxburghii	Rroxscaffold_7G00174370
rosa_rugosa	Rorug06G0233200 Rorug06G0233300
rosa_samantha	Rh6AG345800 Rh6BG353600 Rh6CG360000 Rh6DG346400
rosa_wichuraiana	Rw6G030140

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB7I	CCANNNNNTGG	1 cut(s)	153
AccIII	TCCGGA	2 cut(s)	228, 290
AciI	CCGC	3 cut(s)	318, 839, 1394
AclWI	GGATC	9 cut(s)	504, 518, 791, 804, 823, 902, 915, 1010, 1112
AcoI	YGGCCR	1 cut(s)	462
AcsI	RAATTY	3 cut(s)	419, 1134, 1161
AcuI	CTGAAG	2 cut(s)	854, 1110
AfaI	GTAC	4 cut(s)	33, 599, 930, 1002
AfeI	AGCGCT	1 cut(s)	1036
AfiI	CCNNNNNNNGG	4 cut(s)	153, 806, 838, 1315
AgsI	TTSAA	6 cut(s)	633, 774, 848, 1125, 1268, 1456
AhdI	GACNNNNNGTC	1 cut(s)	815
AjiI	CACGTC	2 cut(s)	337, 1414
AloI	GAACNNNNNNTCC	4 cut(s)	964, 996, 1044, 1076
AluBI	AGCT	6 cut(s)	245, 272, 374, 806, 1220, 1426
AluI	AGCT	6 cut(s)	245, 272, 374, 806, 1220, 1426
Alw21I	GWGCWC	1 cut(s)	439
Alw26I	GTCTC	3 cut(s)	334, 419, 1099
AlwI	GGATC	9 cut(s)	504, 518, 791, 804, 823, 902, 915, 1010, 1112
AlwNI	CAGNNNCTG	1 cut(s)	527
Aor13HI	TCCGGA	2 cut(s)	228, 290
Aor51HI	AGCGCT	1 cut(s)	1036
AoxI	GGCC	2 cut(s)	163, 462
ApeKI	GCWGC	8 cut(s)	242, 260, 348, 374, 884, 1037, 1217, 1426
ApoI	RAATTY	3 cut(s)	419, 1134, 1161
Asp700I	GAANNNNTTC	2 cut(s)	224, 1059
AspLEI	GCGC	1 cut(s)	1037
AspS9I	GGNCC	1 cut(s)	163
AsuC2I	CCSGG	2 cut(s)	265, 1331
AsuHPI	GGTGA	1 cut(s)	218
BalI	TGGCCA	1 cut(s)	464
BamHI	GGATCC	2 cut(s)	796, 907
Bbv12I	GWGCWC	1 cut(s)	439
BbvI	GCAGC	8 cut(s)	247, 254, 360, 361, 871, 1024, 1229, 1413
BccI	CCATC	5 cut(s)	126, 226, 499, 1092, 1238
BceAI	ACGGC	3 cut(s)	315, 483, 1407
BcgI	CGANNNNNNTGC	6 cut(s)	25, 53, 59, 87, 554, 588
BclI	TGATCA	1 cut(s)	187
BcnI	CCSGG	2 cut(s)	265, 1331
BcoDI	GTCTC	3 cut(s)	334, 419, 1099
BfaI	CTAG	2 cut(s)	608, 803
BfmI	CTRYAG	2 cut(s)	531, 1197
BfoI	RGCGCY	1 cut(s)	1038
Bme1390I	CCNGG	2 cut(s)	265, 1331
BmeRI	GACNNNNNGTC	1 cut(s)	815
BmgBI	CACGTC	2 cut(s)	337, 1414
BmgT120I	GGNCC	1 cut(s)	163
BmiI	GGNNCC	3 cut(s)	384, 798, 909
BmrFI	CCNGG	2 cut(s)	265, 1331
BmrI	ACTGGG	3 cut(s)	81, 861, 1137
BmsI	GCATC	8 cut(s)	202, 304, 721, 727, 865, 871, 1288, 1339
BmuI	ACTGGG	3 cut(s)	81, 861, 1137
BpuEI	CTTGAG	1 cut(s)	982
BpuMI	CCSGG	2 cut(s)	265, 1331
BsaBI	GATNNNNATC	2 cut(s)	117, 192
BsaI	GGTCTC	1 cut(s)	334
BsaJI	CCNNGG	3 cut(s)	465, 778, 1329
BsaWI	WCCGGW	3 cut(s)	203, 228, 290
BsaXI	ACNNNNNCTCC	4 cut(s)	964, 994, 1044, 1074
Bsc4I	CCNNNNNNNGG	4 cut(s)	153, 806, 838, 1315
Bse1I	ACTGG	6 cut(s)	87, 261, 662, 856, 879, 1143
Bse3DI	GCAATG	3 cut(s)	306, 477, 1359
Bse8I	GATNNNNATC	2 cut(s)	117, 192
BseAI	TCCGGA	2 cut(s)	228, 290
BseDI	CCNNGG	3 cut(s)	465, 778, 1329
BseGI	GGATG	3 cut(s)	237, 886, 1084
BseJI	GATNNNNATC	2 cut(s)	117, 192
BseLI	CCNNNNNNNGG	4 cut(s)	153, 806, 838, 1315
BseMI	GCAATG	3 cut(s)	306, 477, 1359
BseMII	CTCAG	3 cut(s)	605, 1089, 1424
BseNI	ACTGG	6 cut(s)	87, 261, 662, 856, 879, 1143
BseRI	GAGGAG	1 cut(s)	69
BseXI	GCAGC	8 cut(s)	247, 254, 360, 361, 871, 1024, 1229, 1413
BseYI	CCCAGC	3 cut(s)	102, 674, 892
BsgI	GTGCAG	2 cut(s)	747, 1023
Bsh1285I	CGRYCG	1 cut(s)	144
BshFI	GGCC	2 cut(s)	165, 464
BsiEI	CGRYCG	1 cut(s)	144
BsiHKAI	GWGCWC	1 cut(s)	439
BsiSI	CCGG	5 cut(s)	204, 229, 264, 291, 1331
BslFI	GGGAC	1 cut(s)	75
BslI	CCNNNNNNNGG	4 cut(s)	153, 806, 838, 1315
BsmAI	GTCTC	3 cut(s)	334, 419, 1099
BsmFI	GGGAC	1 cut(s)	75
BsmI	GAATGC	1 cut(s)	1419
BsnI	GGCC	2 cut(s)	165, 464
Bso31I	GGTCTC	1 cut(s)	334
Bsp1286I	GDGCHC	1 cut(s)	439
Bsp13I	TCCGGA	2 cut(s)	228, 290
Bsp1407I	TGTACA	2 cut(s)	928, 1000
BspACI	CCGC	3 cut(s)	318, 839, 1394
BspANI	GGCC	2 cut(s)	165, 464
BspCNI	CTCAG	3 cut(s)	606, 1090, 1425
BspEI	TCCGGA	2 cut(s)	228, 290
BspLI	GGNNCC	3 cut(s)	384, 798, 909
BspMAI	CTGCAG	1 cut(s)	1201
BspPI	GGATC	9 cut(s)	504, 518, 791, 804, 823, 902, 915, 1010, 1112
BspTNI	GGTCTC	1 cut(s)	334
BsrDI	GCAATG	3 cut(s)	306, 477, 1359
BsrGI	TGTACA	2 cut(s)	928, 1000
BsrI	ACTGG	6 cut(s)	87, 261, 662, 856, 879, 1143
BssECI	CCNNGG	3 cut(s)	465, 778, 1329
BssT1I	CCWWGG	1 cut(s)	778
Bst4CI	ACNGT	5 cut(s)	153, 416, 625, 1452, 1460
Bst6I	CTCTTC	2 cut(s)	87, 204
BstAPI	GCANNNNNTGC	2 cut(s)	488, 527
BstAUI	TGTACA	2 cut(s)	928, 1000
BstC8I	GCNNGC	4 cut(s)	45, 489, 823, 1180
BstDEI	CTNAG	4 cut(s)	344, 614, 1098, 1433
BstDSI	CCRYGG	1 cut(s)	465
BstF5I	GGATG	3 cut(s)	237, 886, 1084
BstH2I	RGCGCY	1 cut(s)	1038
BstHHI	GCGC	1 cut(s)	1037
BstMAI	GTCTC	3 cut(s)	334, 419, 1099
BstMCI	CGRYCG	1 cut(s)	144
BstMWI	GCNNNNNNNGC	9 cut(s)	269, 308, 317, 380, 488, 527, 893, 974, 1370
BstNSI	RCATGY	2 cut(s)	491, 1182
BstSCI	CCNGG	2 cut(s)	263, 1329
BstSFI	CTRYAG	2 cut(s)	531, 1197
BstV1I	GCAGC	8 cut(s)	247, 254, 360, 361, 871, 1024, 1229, 1413
BstX2I	RGATCY	5 cut(s)	496, 523, 796, 907, 1117
BstYI	RGATCY	5 cut(s)	496, 523, 796, 907, 1117
BsuRI	GGCC	2 cut(s)	165, 464
BtgI	CCRYGG	1 cut(s)	465
BtgZI	GCGATG	2 cut(s)	179, 941
BtrI	CACGTC	2 cut(s)	337, 1414
BtsCI	GGATG	3 cut(s)	237, 886, 1084
BtsIMutI	CAGTG	3 cut(s)	611, 1150, 1465
Cac8I	GCNNGC	4 cut(s)	45, 489, 823, 1180
CaiI	CAGNNNCTG	1 cut(s)	527
CfoI	GCGC	1 cut(s)	1037
Cfr13I	GGNCC	1 cut(s)	163
CseI	GACGC	1 cut(s)	1418
Csp6I	GTAC	4 cut(s)	32, 598, 929, 1001
CspCI	CAANNNNNGTGG	2 cut(s)	1341, 1376
CviQI	GTAC	4 cut(s)	32, 598, 929, 1001
DdeI	CTNAG	4 cut(s)	344, 614, 1098, 1433
DraI	TTTAAA	1 cut(s)	370
DriI	GACNNNNNGTC	1 cut(s)	815
EaeI	YGGCCR	1 cut(s)	462
Eam1104I	CTCTTC	2 cut(s)	87, 204
Eam1105I	GACNNNNNGTC	1 cut(s)	815
EarI	CTCTTC	2 cut(s)	87, 204
Eco130I	CCWWGG	1 cut(s)	778
Eco31I	GGTCTC	1 cut(s)	334
Eco47III	AGCGCT	1 cut(s)	1036
Eco57I	CTGAAG	2 cut(s)	854, 1110
EcoT14I	CCWWGG	1 cut(s)	778
EcoT22I	ATGCAT	1 cut(s)	715
ErhI	CCWWGG	1 cut(s)	778
FalI	AAGNNNNNCTT	2 cut(s)	31, 63
FaqI	GGGAC	1 cut(s)	75
FauI	CCCGC	1 cut(s)	832
FauNDI	CATATG	1 cut(s)	406
FbaI	TGATCA	1 cut(s)	187
FokI	GGATG	3 cut(s)	244, 893, 1071
FspBI	CTAG	2 cut(s)	608, 803
GlaI	GCGC	1 cut(s)	1036
GsaI	CCCAGC	3 cut(s)	106, 678, 896
HaeII	RGCGCY	1 cut(s)	1038
HaeIII	GGCC	2 cut(s)	165, 464
HapII	CCGG	5 cut(s)	204, 229, 264, 291, 1331
HgaI	GACGC	1 cut(s)	1418
HhaI	GCGC	1 cut(s)	1037
Hin6I	GCGC	1 cut(s)	1035
HinP1I	GCGC	1 cut(s)	1035
HincII	GTYRAC	1 cut(s)	589
HindII	GTYRAC	1 cut(s)	589
HindIII	AAGCTT	1 cut(s)	270
HinfI	GANTC	8 cut(s)	6, 12, 113, 427, 444, 1072, 1402, 1438
HpaII	CCGG	5 cut(s)	204, 229, 264, 291, 1331
HphI	GGTGA	1 cut(s)	218
Hpy166II	GTNNAC	3 cut(s)	58, 589, 695
Hpy188I	TCNGA	7 cut(s)	11, 334, 387, 861, 1071, 1099, 1434
Hpy188III	TCNNGA	6 cut(s)	229, 291, 794, 832, 1088, 1346
Hpy8I	GTNNAC	3 cut(s)	58, 589, 695
Hpy99I	CGWCG	5 cut(s)	338, 1068, 1243, 1402, 1418
HpyAV	CCTTC	4 cut(s)	641, 829, 940, 1131
HpyCH4III	ACNGT	5 cut(s)	153, 416, 625, 1452, 1460
HpyCH4IV	ACGT	3 cut(s)	54, 336, 1413
HpyF10VI	GCNNNNNNNGC	9 cut(s)	269, 308, 317, 380, 488, 527, 893, 974, 1370
HpyF3I	CTNAG	4 cut(s)	344, 614, 1098, 1433
HpySE526I	ACGT	3 cut(s)	54, 336, 1413
HspAI	GCGC	1 cut(s)	1035
Kpn2I	TCCGGA	2 cut(s)	228, 290
Ksp22I	TGATCA	1 cut(s)	187
LmnI	GCTCC	2 cut(s)	388, 434
Lsp1109I	GCAGC	8 cut(s)	247, 254, 360, 361, 871, 1024, 1229, 1413
LweI	GCATC	8 cut(s)	202, 304, 721, 727, 865, 871, 1288, 1339
MaeI	CTAG	2 cut(s)	608, 803
MaeII	ACGT	3 cut(s)	54, 336, 1413
MaeIII	GTNAC	3 cut(s)	281, 1287, 1460
MboII	GAAGA	4 cut(s)	74, 221, 232, 1160
MfeI	CAATTG	1 cut(s)	924
MflI	RGATCY	5 cut(s)	496, 523, 796, 907, 1117
MhlI	GDGCHC	1 cut(s)	439
MlsI	TGGCCA	1 cut(s)	464
MluNI	TGGCCA	1 cut(s)	464
MlyI	GAGTC	1 cut(s)	421
MmeI	TCCRAC	3 cut(s)	232, 357, 745
Mox20I	TGGCCA	1 cut(s)	464
Mph1103I	ATGCAT	1 cut(s)	715
MroI	TCCGGA	2 cut(s)	228, 290
MroXI	GAANNNNTTC	2 cut(s)	224, 1059
MscI	TGGCCA	1 cut(s)	464
MseI	TTAA	2 cut(s)	369, 642
MslI	CAYNNNNRTG	4 cut(s)	405, 558, 723, 861
Msp20I	TGGCCA	1 cut(s)	464
MspA1I	CMGCKG	3 cut(s)	245, 1220, 1426
MspI	CCGG	5 cut(s)	204, 229, 264, 291, 1331
MspR9I	CCNGG	2 cut(s)	265, 1331
MunI	CAATTG	1 cut(s)	924
Mva1269I	GAATGC	1 cut(s)	1419
MwoI	GCNNNNNNNGC	9 cut(s)	269, 308, 317, 380, 488, 527, 893, 974, 1370
NciI	CCSGG	2 cut(s)	265, 1331
NdeI	CATATG	1 cut(s)	406
NlaIV	GGNNCC	3 cut(s)	384, 798, 909
NmeAIII	GCCGAG	1 cut(s)	1399
NmuCI	GTSAC	1 cut(s)	1460
NsiI	ATGCAT	1 cut(s)	715
NspI	RCATGY	2 cut(s)	491, 1182
PaeI	GCATGC	2 cut(s)	491, 1182
PcsI	WCGNNNNNNNCGW	2 cut(s)	51, 1397
PctI	GAATGC	1 cut(s)	1419
PdmI	GAANNNNTTC	2 cut(s)	224, 1059
PfeI	GAWTC	7 cut(s)	6, 12, 113, 444, 1072, 1402, 1438
PflMI	CCANNNNNTGG	1 cut(s)	153
Ple19I	CGATCG	1 cut(s)	144
PleI	GAGTC	1 cut(s)	421
PpsI	GAGTC	1 cut(s)	421
PspFI	CCCAGC	3 cut(s)	102, 674, 892
PspN4I	GGNNCC	3 cut(s)	384, 798, 909
PspPI	GGNCC	1 cut(s)	163
PstI	CTGCAG	1 cut(s)	1201
PstNI	CAGNNNCTG	1 cut(s)	527
PsuI	RGATCY	5 cut(s)	496, 523, 796, 907, 1117
PvuI	CGATCG	1 cut(s)	144
PvuII	CAGCTG	3 cut(s)	245, 1220, 1426
RsaI	GTAC	4 cut(s)	33, 599, 930, 1002
RsaNI	GTAC	4 cut(s)	32, 598, 929, 1001
RseI	CAYNNNNRTG	4 cut(s)	405, 558, 723, 861
SaqAI	TTAA	2 cut(s)	369, 642
Sau96I	GGNCC	1 cut(s)	163
SchI	GAGTC	1 cut(s)	421
ScrFI	CCNGG	2 cut(s)	265, 1331
SduI	GDGCHC	1 cut(s)	439
SfaNI	GCATC	8 cut(s)	202, 304, 721, 727, 865, 871, 1288, 1339
SfcI	CTRYAG	2 cut(s)	531, 1197
SmiMI	CAYNNNNRTG	4 cut(s)	405, 558, 723, 861
SmlI	CTYRAG	1 cut(s)	961
SmoI	CTYRAG	1 cut(s)	961
SphI	GCATGC	2 cut(s)	491, 1182
SsiI	CCGC	3 cut(s)	318, 839, 1394
SspMI	CTAG	2 cut(s)	608, 803
StyD4I	CCNGG	2 cut(s)	263, 1329
StyI	CCWWGG	1 cut(s)	778
TaaI	ACNGT	5 cut(s)	153, 416, 625, 1452, 1460
TaiI	ACGT	3 cut(s)	57, 339, 1416
TaqI	TCGA	5 cut(s)	111, 140, 936, 1241, 1345
TaqII	GACCGA	1 cut(s)	316
TatI	WGTACW	2 cut(s)	928, 1000
TauI	GCSGC	2 cut(s)	320, 1397
TfiI	GAWTC	7 cut(s)	6, 12, 113, 444, 1072, 1402, 1438
Tru1I	TTAA	2 cut(s)	369, 642
Tru9I	TTAA	2 cut(s)	369, 642
TscAI	CASTG	3 cut(s)	618, 1150, 1465
TseFI	GTSAC	1 cut(s)	1460
TseI	GCWGC	8 cut(s)	242, 260, 348, 374, 884, 1037, 1217, 1426
Tsp45I	GTSAC	1 cut(s)	1460
TspDTI	ATGAA	4 cut(s)	716, 929, 972, 1096
TspRI	CASTG	3 cut(s)	618, 1150, 1465
Van91I	CCANNNNNTGG	1 cut(s)	153
XapI	RAATTY	3 cut(s)	419, 1134, 1161
XceI	RCATGY	2 cut(s)	491, 1182
XmnI	GAANNNNTTC	2 cut(s)	224, 1059
XspI	CTAG	2 cut(s)	608, 803
Zsp2I	ATGCAT	1 cut(s)	715

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.