Rmu_sc0014786.1_g000003

Metal tolerance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0014786.1
OG0003006
Physical Location & Seq
Forward (+)
18202 .. 19294
1093 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0014786.1_g000003.1.cds

Sequence Viewer

Length: 615 bp
atggtctggacagggtggaagaagaagtggtggaggaggaacatgcaagaagaagtggaggatgataaagagcatactggcacactgggtagtgcaccttcaaacttccagacttgtagctcaagctcccttttctcagaaccagaaaaaccccaaatcaaatacacaacgcattttggtcctgatcaagaattcatcctgaacgatctccttcaagcttcaactatgggattcagatttcagaatctcaattccataggcacaaggctcgctgctctatacatagccaagcattctcacccacaccccacaataatctcccaaaccctcaattcccatctccaagaccattctttgattctccatcaaaacccgatttactcaattcccagaagatggcatttgggccattcccacggccctgaggaggattatggcaaagagggcgagaagatcttccggctgggattggctgctgatattggtcttgcttccggaaaagctttgactggttacttatctggcagcaccgccataattgccgacgccgcccattcagtctccgacgtggtaaatttttattttttacagttgaattttgtttttcttggttaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cation_efflux ZT_dimer

Protein Analysis

204

Amino Acids

22.94

Weight (kDa)

6.44

Isoelectric Point (pI)

33.37

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 396
AccIII TCCGGA 1 cut(s) 494
AciI CCGC 2 cut(s) 531, 549
AcsI RAATTY 3 cut(s) 191, 574, 595
AcyI GRCGYC 1 cut(s) 546
AfiI CCNNNNNNNGG 2 cut(s) 396, 427
AgsI TTSAA 4 cut(s) 102, 215, 222, 595
AjiI CACGTC 1 cut(s) 568
AluBI AGCT 4 cut(s) 120, 126, 218, 503
AluI AGCT 4 cut(s) 120, 126, 218, 503
Alw21I GWGCWC 1 cut(s) 97
Alw26I GTCTC 1 cut(s) 565
Alw44I GTGCAC 1 cut(s) 93
Aor13HI TCCGGA 1 cut(s) 494
AoxI GGCC 2 cut(s) 406, 418
ApaLI GTGCAC 1 cut(s) 93
ApeKI GCWGC 3 cut(s) 272, 473, 525
ApoI RAATTY 3 cut(s) 191, 574, 595
Asp700I GAANNNNTTC 1 cut(s) 455
AspS9I GGNCC 3 cut(s) 179, 406, 419
AsuHPI GGTGA 1 cut(s) 290
AvaII GGWCC 1 cut(s) 179
AxyI CCTNAGG 1 cut(s) 423
BaeGI GKGCMC 1 cut(s) 97
BarI GAAGNNNNNNTAC 2 cut(s) 82, 114
Bbv12I GWGCWC 1 cut(s) 97
BbvI GCAGC 3 cut(s) 259, 460, 537
BccI CCATC 3 cut(s) 345, 372, 390
BceAI ACGGC 1 cut(s) 433
BcgI CGANNNNNNTGC 2 cut(s) 250, 284
BclI TGATCA 1 cut(s) 184
BcoDI GTCTC 1 cut(s) 565
BglII AGATCT 1 cut(s) 453
BisI GCNGC 4 cut(s) 273, 474, 526, 549
BlsI GCNGC 4 cut(s) 274, 475, 527, 550
Bme18I GGWCC 1 cut(s) 179
BmgBI CACGTC 1 cut(s) 568
BmgT120I GGNCC 3 cut(s) 179, 406, 419
BmrI ACTGGG 1 cut(s) 95
BmuI ACTGGG 1 cut(s) 95
BpuEI CTTGAG 1 cut(s) 106
BsaHI GRCGYC 1 cut(s) 546
BsaJI CCNNGG 1 cut(s) 415
BsaWI WCCGGW 1 cut(s) 494
Bsc4I CCNNNNNNNGG 2 cut(s) 396, 427
Bse1I ACTGG 3 cut(s) 82, 90, 514
Bse21I CCTNAGG 1 cut(s) 423
BseAI TCCGGA 1 cut(s) 494
BseDI CCNNGG 1 cut(s) 415
BseGI GGATG 2 cut(s) 67, 195
BseLI CCNNNNNNNGG 2 cut(s) 396, 427
BseMII CTCAG 2 cut(s) 150, 414
BseNI ACTGG 3 cut(s) 82, 90, 514
BseRI GAGGAG 2 cut(s) 49, 440
BseSI GKGCMC 1 cut(s) 97
BseXI GCAGC 3 cut(s) 259, 460, 537
BseYI CCCAGC 1 cut(s) 463
BshFI GGCC 2 cut(s) 408, 420
BsiHKAI GWGCWC 1 cut(s) 97
BsiSI CCGG 2 cut(s) 460, 495
BslI CCNNNNNNNGG 2 cut(s) 396, 427
BsmAI GTCTC 1 cut(s) 565
BsmI GAATGC 1 cut(s) 292
BsnI GGCC 2 cut(s) 408, 420
Bsp1286I GDGCHC 1 cut(s) 97
Bsp13I TCCGGA 1 cut(s) 494
Bsp143I GATC 3 cut(s) 184, 205, 453
BspACI CCGC 2 cut(s) 531, 549
BspANI GGCC 2 cut(s) 408, 420
BspCNI CTCAG 2 cut(s) 149, 415
BspEI TCCGGA 1 cut(s) 494
BsrI ACTGG 3 cut(s) 82, 90, 514
BssECI CCNNGG 1 cut(s) 415
BssMI GATC 3 cut(s) 184, 205, 453
BssNI GRCGYC 1 cut(s) 546
Bst4CI ACNGT 1 cut(s) 591
BstACI GRCGYC 1 cut(s) 546
BstC8I GCNNGC 1 cut(s) 270
BstDEI CTNAG 2 cut(s) 136, 423
BstDSI CCRYGG 1 cut(s) 415
BstF5I GGATG 2 cut(s) 67, 195
BstKTI GATC 3 cut(s) 187, 208, 456
BstMAI GTCTC 1 cut(s) 565
BstMBI GATC 3 cut(s) 184, 205, 453
BstMWI GCNNNNNNNGC 3 cut(s) 444, 539, 548
BstNSI RCATGY 1 cut(s) 46
BstSLI GKGCMC 1 cut(s) 97
BstV1I GCAGC 3 cut(s) 259, 460, 537
BstX2I RGATCY 1 cut(s) 453
BstYI RGATCY 1 cut(s) 453
Bsu36I CCTNAGG 1 cut(s) 423
BsuRI GGCC 2 cut(s) 408, 420
BtgI CCRYGG 1 cut(s) 415
BtrI CACGTC 1 cut(s) 568
BtsCI GGATG 2 cut(s) 67, 195
BtsIMutI CAGTG 1 cut(s) 83
Cac8I GCNNGC 1 cut(s) 270
Cfr13I GGNCC 3 cut(s) 179, 406, 419
CseI GACGC 1 cut(s) 554
CviAII CATG 1 cut(s) 43
DdeI CTNAG 2 cut(s) 136, 423
DpnI GATC 3 cut(s) 186, 207, 455
DpnII GATC 3 cut(s) 184, 205, 453
Eco47I GGWCC 1 cut(s) 179
Eco81I CCTNAGG 1 cut(s) 423
EcoRI GAATTC 1 cut(s) 191
FaeI CATG 1 cut(s) 46
FaiI YATR 8 cut(s) 44, 75, 227, 257, 280, 284, 435, 536
FatI CATG 1 cut(s) 42
FbaI TGATCA 1 cut(s) 184
Fnu4HI GCNGC 4 cut(s) 273, 474, 526, 549
FokI GGATG 2 cut(s) 74, 182
Fsp4HI GCNGC 4 cut(s) 273, 474, 526, 549
GluI GCNGC 4 cut(s) 273, 474, 526, 549
GsaI CCCAGC 1 cut(s) 467
HaeIII GGCC 2 cut(s) 408, 420
HapII CCGG 2 cut(s) 460, 495
HgaI GACGC 1 cut(s) 554
Hin1I GRCGYC 1 cut(s) 546
Hin1II CATG 1 cut(s) 46
HindIII AAGCTT 2 cut(s) 216, 501
HinfI GANTC 3 cut(s) 231, 244, 358
HpaII CCGG 2 cut(s) 460, 495
HphI GGTGA 1 cut(s) 290
Hpy166II GTNNAC 1 cut(s) 95
Hpy188I TCNGA 4 cut(s) 139, 236, 243, 565
Hpy188III TCNNGA 6 cut(s) 7, 109, 182, 188, 199, 495
Hpy8I GTNNAC 1 cut(s) 95
Hpy99I CGWCG 2 cut(s) 548, 569
HpyAV CCTTC 2 cut(s) 108, 221
HpyCH4III ACNGT 1 cut(s) 591
HpyCH4IV ACGT 1 cut(s) 567
HpyCH4V TGCA 2 cut(s) 46, 95
HpyF10VI GCNNNNNNNGC 3 cut(s) 444, 539, 548
HpyF3I CTNAG 2 cut(s) 136, 423
HpySE526I ACGT 1 cut(s) 567
Hsp92I GRCGYC 1 cut(s) 546
Hsp92II CATG 1 cut(s) 46
Kpn2I TCCGGA 1 cut(s) 494
Ksp22I TGATCA 1 cut(s) 184
Kzo9I GATC 3 cut(s) 184, 205, 453
LmnI GCTCC 1 cut(s) 131
Lsp1109I GCAGC 3 cut(s) 259, 460, 537
MaeII ACGT 1 cut(s) 567
MaeIII GTNAC 1 cut(s) 512
MalI GATC 3 cut(s) 186, 207, 455
MboI GATC 3 cut(s) 184, 205, 453
MboII GAAGA 6 cut(s) 31, 34, 62, 405, 448, 463
MflI RGATCY 1 cut(s) 453
MhlI GDGCHC 1 cut(s) 97
MluCI AATT 7 cut(s) 191, 250, 331, 384, 537, 574, 595
MmeI TCCRAC 1 cut(s) 588
MnlI CCTC 7 cut(s) 27, 30, 52, 338, 418, 421, 436
MroI TCCGGA 1 cut(s) 494
MroXI GAANNNNTTC 1 cut(s) 455
MseI TTAA 1 cut(s) 613
MspI CCGG 2 cut(s) 460, 495
Mva1269I GAATGC 1 cut(s) 292
MwoI GCNNNNNNNGC 3 cut(s) 444, 539, 548
NdeII GATC 3 cut(s) 184, 205, 453
NlaIII CATG 1 cut(s) 46
NspI RCATGY 1 cut(s) 46
PctI GAATGC 1 cut(s) 292
PdmI GAANNNNTTC 1 cut(s) 455
PfeI GAWTC 3 cut(s) 231, 244, 358
PflMI CCANNNNNTGG 1 cut(s) 396
PkrI GCNGC 4 cut(s) 274, 475, 527, 550
PspFI CCCAGC 1 cut(s) 463
PspPI GGNCC 3 cut(s) 179, 406, 419
PsuI RGATCY 1 cut(s) 453
SaqAI TTAA 1 cut(s) 613
SatI GCNGC 4 cut(s) 273, 474, 526, 549
Sau3AI GATC 3 cut(s) 184, 205, 453
Sau96I GGNCC 3 cut(s) 179, 406, 419
SduI GDGCHC 1 cut(s) 97
SetI ASST 6 cut(s) 100, 122, 128, 220, 505, 570
SinI GGWCC 1 cut(s) 179
SmlI CTYRAG 1 cut(s) 121
SmoI CTYRAG 1 cut(s) 121
Sse9I AATT 7 cut(s) 191, 250, 331, 384, 537, 574, 595
SsiI CCGC 2 cut(s) 531, 549
TaaI ACNGT 1 cut(s) 591
TaiI ACGT 1 cut(s) 570
TasI AATT 7 cut(s) 191, 250, 331, 384, 537, 574, 595
TauI GCSGC 1 cut(s) 551
TfiI GAWTC 3 cut(s) 231, 244, 358
Tru1I TTAA 1 cut(s) 613
Tru9I TTAA 1 cut(s) 613
TscAI CASTG 1 cut(s) 90
TseI GCWGC 3 cut(s) 272, 473, 525
TspDTI ATGAA 1 cut(s) 184
TspRI CASTG 1 cut(s) 90
Van91I CCANNNNNTGG 1 cut(s) 396
VneI GTGCAC 1 cut(s) 93
VpaK11BI GGWCC 1 cut(s) 179
XapI RAATTY 3 cut(s) 191, 574, 595
XceI RCATGY 1 cut(s) 46
XmnI GAANNNNTTC 1 cut(s) 455
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.