Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr04

Orthogroup ID

OG0005480

Physical Location & Seq

Genomic Coordinates Reverse (-)

415256 .. 421007

Gene Structure 5752 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD04G1002900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1959 bp

ATGGCTGGAATCGATGTGTCCAAGTATGATCATAGCCCTGTGCACAAGGCCATAGTCACGAGGGATTATGCCGGTCTCAAGAGGATTCTATCTGGTCTCCCGCGGCTTTGTAACCCTGCTGAGATTCGAACTGAATCAGCTTCATTAGCTGAGGAAGAGAAGGCTGATGCCATTGCTGCTGTCATTGATCGGCGAGATGTCCCAAACCGTGAGACCCCTCTTCACTTGGCTGTAAAACTTGGTGACCAGACAGCAACCGAAATGCTTATGGTTGCTGGCGCGGATTGGAGCTTGCAGAATGAACATGGATGGAGTGCACTCCAGGAAGCAATTTGCAATAGAGAAGAAGCAATTTCCATGATCATAGTTAGGCACTACCAGCCACTGGCTTGGGCAAAATGGTGCAGAAGGTTACCTCGTTTAATTGGAACAATGCGGAGGATGAGGGATTTCTACATGGAAATTACATTCCGCTTTGAGAGTTCTGTTATACCTTTCATTTCGAGGATTGCCCCATCAGATACTTACAAGATCTGGAAAAAGGGTGCAAATTTGAGAGCAGATATGACATTGGCAGGTTTTGATGGTTTGCGAATTCAGCGTTCTGATCAGAGTGTTCTTTTCCTTGGTGATGGGTCTGAGGATGGTAAGGTGCCTCCCGGGTCACTTTGCATGATCTCACACAAGGATAAGGAGGTGATGAATGCTTTGGATGGTGCTGGATCTCAAGCAACTGAAGAGGAGGTTCGGCAAGAGGTGGTTGCAATGTCTCAGACTAATATATTCAGGCCTGGGGTTGATGTGACTCAAGCAGTACTTTTACCTCAAATGACCTGGAGGCGGCAGGAGAAAACAGAAATGGTTGGCCCATGGAAGGCTAAGGTATATGATATGCATAATGTGGTTGTGAGTATGAAATCTAGAAGGGTCCCCGGGGCTATGACAGATGACGAGCTCTTCTCAACCTGCAATGAAAATGAAACAGAAAGTGAGGAGTTTAATGATATTCTGACAGAGGATGAGAGGAAGCAACTTGAAAATGCACTTAAATTGGATTCGTCAGACTTGTCAAACGAAAATGGTGATGGGATTATTGAGCATCGTCGTAGTTGTTATGAGCACAGGGAGGTTCCCATTGAAAATGTTAGTAGTGATAGAAATGGAGAGAATAAGCAGGAAAAGAAAGGATGGTTTGGTGGATGGAGGAAACGGGATACTAAAAGTGAAGGGCAGAAAAAGATTGTGCCGCCTAGAAGTTCTCTCTGTGTAGATGAGAAGGTGAGTGATCTGCTAGGGGATTCTCCTTCAAGAAATCAAAGCAGGCCTGGAAGAAATAGTGTGGACATTGTTGTGAAGGGGGATGATCATCGGAGAGGAAGGGATATGAAGGCGTCTTCTTCCGCAAGTTCTGATAACAGGAATCGTCACAAGGATGCTAGCAAGGAGAATGAGTATAAGAAAGGATTAAGGCCAATCCTCTGGCTTTCCCCAAGCTTTCCATTACAAACAGAGGAACTGTTGCCATTACTTGATATTTTAGCAAACAAGGTTAAGGCAATTCGTCGTTTGAGGGAGCTGCTTACAACTAAGCTTCCAATGGGTACATTTCCAGTCAAGGTTGCTATCCCAGTGGTTCCTACTATCAGAGTAATTGTCACCTTCACAAAGCTTGAAGAATTACCACCAATGGATGAGTTTGCAACACCGCCATCAAGCCCCCCTGGCAGGGAGAGCCCAGGTTCGATGCAATCGGCAAGTTCATCTTGGTTTCAGTGGATAAGAGCTCCTTACCAGCGCCCCAGCGCGTCCCCTACTGGTTCTTTCAGTAAGATAGAAAGTATTCAAGACCCGTTTGCAATTCCGTCAGAATATACATGGGTTTCTGTGGAAGCAAAGAAAAAGAAAATGCAGGAGAAGAACAAGTCAAAGAAAGGGAAAAGTCAGAGTCACCAAAAATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Ank_2 Ank_5 GPCR_chapero_1

Protein Analysis

653

Amino Acids

73.54

Weight (kDa)

8.69

Isoelectric Point (pI)

63.95

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
ANKRD13_C	PF11904	186 - 625	1e-117	ANKRD13 C-terminal

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0005480)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G04780
fragaria_vesca	FvH4_4g00280 FvH4_4g00280 FvH4_4g00280 FvH4_4g00280
malus_domestica	MD04G1002900.v1.1 MD13G1285000.v1.1
prunus_persica	Prupe.1G002800_v2.0.a1 Prupe.1G002800_v2.0.a1 Prupe.1G002800_v2.0.a1 Prupe.1G002800_v2.0.a1 Prupe.1G002800_v2.0.a1
pyrus_communis	pycom04g00300 pycom420g00660
rosa_chinensis	RchiOBHm_Chr4g0385121
rosa_laevigata	RLG00000010209 RLG00000010228 RLG00000010256
rosa_multiflora	Rmu_ssc0000158.1_g000038
rosa_roxburghii	Rroxscaffold_5G00333030 Rroxscaffold_5G00364810
rosa_rugosa	Rorug03G0299200 Rorug03G0299300 Rorug03G0299300
rosa_samantha	Rh4AG002800 Rh4BG002300 Rh4CG003100 Rh4DG002600
rosa_wichuraiana	Rw4G000650

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	2 cut(s)	566, 974
AccB1I	GGYRCC	1 cut(s)	652
AccB7I	CCANNNNNTGG	1 cut(s)	385
AccII	CGCG	3 cut(s)	103, 281, 1805
AciI	CCGC	9 cut(s)	101, 103, 281, 436, 472, 841, 1247, 1401, 1706
AclWI	GGATC	1 cut(s)	730
AcsI	RAATTY	2 cut(s)	550, 594
AcuI	CTGAAG	1 cut(s)	756
AcyI	GRCGYC	1 cut(s)	1391
AfaI	GTAC	2 cut(s)	816, 1603
AfiI	CCNNNNNNNGG	5 cut(s)	385, 840, 874, 1725, 1726
AgsI	TTSAA	5 cut(s)	1037, 1139, 1308, 1673, 1844
AjnI	CCWGG	6 cut(s)	321, 790, 833, 1324, 1720, 1735
AluBI	AGCT	9 cut(s)	140, 149, 291, 955, 1494, 1576, 1591, 1669, 1784
AluI	AGCT	9 cut(s)	140, 149, 291, 955, 1494, 1576, 1591, 1669, 1784
Alw21I	GWGCWC	5 cut(s)	45, 319, 957, 1122, 1786
Alw26I	GTCTC	4 cut(s)	80, 101, 206, 774
Alw44I	GTGCAC	2 cut(s)	41, 315
AlwI	GGATC	1 cut(s)	730
AlwNI	CAGNNNCTG	1 cut(s)	385
Ama87I	CYCGRG	2 cut(s)	659, 932
AoxI	GGCC	5 cut(s)	48, 788, 865, 1322, 1469
ApaLI	GTGCAC	2 cut(s)	41, 315
ApeKI	GCWGC	2 cut(s)	176, 1576
ApoI	RAATTY	2 cut(s)	550, 594
Asp700I	GAANNNNTTC	1 cut(s)	1839
AspLEI	GCGC	3 cut(s)	281, 1797, 1805
AspS9I	GGNCC	2 cut(s)	866, 928
AsuC2I	CCSGG	4 cut(s)	660, 661, 933, 934
AsuHPI	GGTGA	7 cut(s)	254, 641, 709, 1094, 1291, 1648, 1940
AsuII	TTCGAA	1 cut(s)	127
AsuNHI	GCTAGC	1 cut(s)	1436
AvaI	CYCGRG	2 cut(s)	659, 932
AvaII	GGWCC	1 cut(s)	928
BaeGI	GKGCMC	2 cut(s)	45, 319
BanI	GGYRCC	1 cut(s)	652
BanII	GRGCYC	3 cut(s)	957, 1736, 1786
BauI	CACGAG	1 cut(s)	58
BbsI	GAAGAC	1 cut(s)	1386
Bbv12I	GWGCWC	5 cut(s)	45, 319, 957, 1122, 1786
BbvCI	CCTCAGC	1 cut(s)	150
BbvI	GCAGC	2 cut(s)	163, 1563
BciT130I	CCWGG	6 cut(s)	323, 792, 835, 1326, 1722, 1737
BciVI	GTATCC	1 cut(s)	1207
BclI	TGATCA	4 cut(s)	28, 360, 607, 1363
BcnI	CCSGG	4 cut(s)	660, 661, 933, 934
BcoDI	GTCTC	4 cut(s)	80, 101, 206, 774
BfaI	CTAG	4 cut(s)	921, 1251, 1292, 1437
BfoI	RGCGCY	1 cut(s)	1798
BfuAI	ACCTGC	2 cut(s)	566, 974
BfuI	GTATCC	1 cut(s)	1207
BglI	GCCNNNNNGGC	1 cut(s)	1722
BglII	AGATCT	1 cut(s)	531
BisI	GCNGC	5 cut(s)	104, 177, 842, 1247, 1577
BlsI	GCNGC	5 cut(s)	105, 178, 843, 1248, 1578
BmcAI	AGTACT	1 cut(s)	816
Bme18I	GGWCC	1 cut(s)	928
BmeT110I	CYCGRG	2 cut(s)	659, 932
BmgT120I	GGNCC	2 cut(s)	866, 928
BmiI	GGNNCC	5 cut(s)	654, 929, 930, 1131, 1635
BmrI	ACTGGG	1 cut(s)	1622
BmsI	GCATC	4 cut(s)	157, 1108, 1423, 1734
BmtI	GCTAGC	1 cut(s)	1440
BmuI	ACTGGG	1 cut(s)	1622
BpiI	GAAGAC	1 cut(s)	1386
BplI	GAGNNNNNCTC	2 cut(s)	944, 976
BpmI	CTGGAG	2 cut(s)	305, 856
Bpu10I	CCTNAGC	2 cut(s)	150, 879
Bpu14I	TTCGAA	1 cut(s)	127
BpuEI	CTTGAG	3 cut(s)	62, 711, 792
BpuMI	CCSGG	4 cut(s)	660, 661, 933, 934
Bsa29I	ATCGAT	1 cut(s)	12
BsaBI	GATNNNNATC	1 cut(s)	1365
BsaHI	GRCGYC	1 cut(s)	1391
BsaI	GGTCTC	3 cut(s)	80, 101, 206
BsaXI	ACNNNNNCTCC	2 cut(s)	1436, 1466
Bsc4I	CCNNNNNNNGG	5 cut(s)	385, 840, 874, 1725, 1726
Bse118I	RCCGGY	1 cut(s)	71
Bse1I	ACTGG	4 cut(s)	390, 1610, 1628, 1819
Bse3DI	GCAATG	3 cut(s)	171, 771, 976
Bse8I	GATNNNNATC	1 cut(s)	1365
BseBI	CCWGG	6 cut(s)	323, 792, 835, 1326, 1722, 1737
BseCI	ATCGAT	1 cut(s)	12
BseJI	GATNNNNATC	1 cut(s)	1365
BseLI	CCNNNNNNNGG	5 cut(s)	385, 840, 874, 1725, 1726
BseMI	GCAATG	3 cut(s)	171, 771, 976
BseMII	CTCAG	4 cut(s)	111, 141, 630, 785
BseNI	ACTGG	4 cut(s)	390, 1610, 1628, 1819
BseRI	GAGGAG	2 cut(s)	755, 1007
BseSI	GKGCMC	2 cut(s)	45, 319
BseXI	GCAGC	2 cut(s)	163, 1563
BseYI	CCCAGC	1 cut(s)	1799
BsgI	GTGCAG	1 cut(s)	424
Bsh1236I	CGCG	3 cut(s)	103, 281, 1805
BshFI	GGCC	5 cut(s)	50, 790, 867, 1324, 1471
BshNI	GGYRCC	1 cut(s)	652
BshVI	ATCGAT	1 cut(s)	12
BsiHKAI	GWGCWC	5 cut(s)	45, 319, 957, 1122, 1786
BsiHKCI	CYCGRG	2 cut(s)	659, 932
BsiSI	CCGG	3 cut(s)	72, 660, 933
BslFI	GGGAC	3 cut(s)	185, 914, 1792
BslI	CCNNNNNNNGG	5 cut(s)	385, 840, 874, 1725, 1726
BsmAI	GTCTC	4 cut(s)	80, 101, 206, 774
BsmFI	GGGAC	3 cut(s)	185, 914, 1792
BsmI	GAATGC	1 cut(s)	709
BsnI	GGCC	5 cut(s)	50, 790, 867, 1324, 1471
Bso31I	GGTCTC	3 cut(s)	80, 101, 206
BsoBI	CYCGRG	2 cut(s)	659, 932
Bsp119I	TTCGAA	1 cut(s)	127
Bsp1286I	GDGCHC	6 cut(s)	45, 319, 957, 1122, 1736, 1786
Bsp143I	GATC	9 cut(s)	28, 187, 360, 531, 607, 675, 722, 1285, 1363
Bsp19I	CCATGG	1 cut(s)	869
BspACI	CCGC	9 cut(s)	101, 103, 281, 436, 472, 841, 1247, 1401, 1706
BspANI	GGCC	5 cut(s)	50, 790, 867, 1324, 1471
BspCNI	CTCAG	4 cut(s)	112, 142, 631, 784
BspDI	ATCGAT	1 cut(s)	12
BspFNI	CGCG	3 cut(s)	103, 281, 1805
BspLI	GGNNCC	5 cut(s)	654, 929, 930, 1131, 1635
BspMI	ACCTGC	2 cut(s)	566, 974
BspOI	GCTAGC	1 cut(s)	1440
BspPI	GGATC	1 cut(s)	730
BspQI	GCTCTTC	1 cut(s)	962
BspT104I	TTCGAA	1 cut(s)	127
BspT107I	GGYRCC	1 cut(s)	652
BspTNI	GGTCTC	3 cut(s)	80, 101, 206
BsrDI	GCAATG	3 cut(s)	171, 771, 976
BsrFI	RCCGGY	1 cut(s)	71
BsrI	ACTGG	4 cut(s)	390, 1610, 1628, 1819
BssAI	RCCGGY	1 cut(s)	71
BssMI	GATC	9 cut(s)	28, 187, 360, 531, 607, 675, 722, 1285, 1363
BssNI	GRCGYC	1 cut(s)	1391
BssSI	CACGAG	1 cut(s)	58
BssT1I	CCWWGG	2 cut(s)	625, 869
Bst2BI	CACGAG	1 cut(s)	58
Bst2UI	CCWGG	6 cut(s)	323, 792, 835, 1326, 1722, 1737
Bst4CI	ACNGT	2 cut(s)	209, 1518
Bst6I	CTCTTC	4 cut(s)	150, 225, 732, 962
BstACI	GRCGYC	1 cut(s)	1391
BstBI	TTCGAA	1 cut(s)	127
BstC8I	GCNNGC	4 cut(s)	277, 293, 1322, 1438
BstDEI	CTNAG	6 cut(s)	120, 150, 639, 771, 879, 1587
BstDSI	CCRYGG	2 cut(s)	101, 869
BstEII	GGTNACC	2 cut(s)	242, 411
BstFNI	CGCG	3 cut(s)	103, 281, 1805
BstH2I	RGCGCY	1 cut(s)	1798
BstHHI	GCGC	3 cut(s)	281, 1797, 1805
BstKTI	GATC	9 cut(s)	31, 190, 363, 534, 610, 678, 725, 1288, 1366
BstMAI	GTCTC	4 cut(s)	80, 101, 206, 774
BstMBI	GATC	9 cut(s)	28, 187, 360, 531, 607, 675, 722, 1285, 1363
BstMWI	GCNNNNNNNGC	6 cut(s)	146, 176, 379, 598, 1722, 1731
BstNI	CCWGG	6 cut(s)	323, 792, 835, 1326, 1722, 1737
BstPI	GGTNACC	2 cut(s)	242, 411
BstSLI	GKGCMC	2 cut(s)	45, 319
BstUI	CGCG	3 cut(s)	103, 281, 1805
BstV1I	GCAGC	2 cut(s)	163, 1563
BstV2I	GAAGAC	1 cut(s)	1386
BstX2I	RGATCY	2 cut(s)	531, 722
BstXI	CCANNNNNNTGG	2 cut(s)	390, 1479
BstYI	RGATCY	2 cut(s)	531, 722
Bsu15I	ATCGAT	1 cut(s)	12
BsuI	GTATCC	1 cut(s)	1207
BsuRI	GGCC	5 cut(s)	50, 790, 867, 1324, 1471
BsuTUI	ATCGAT	1 cut(s)	12
BtgI	CCRYGG	2 cut(s)	101, 869
BtsIMutI	CAGTG	3 cut(s)	383, 1635, 1778
BveI	ACCTGC	2 cut(s)	566, 974
Cac8I	GCNNGC	4 cut(s)	277, 293, 1322, 1438
CaiI	CAGNNNCTG	1 cut(s)	385
CfoI	GCGC	3 cut(s)	281, 1797, 1805
Cfr10I	RCCGGY	1 cut(s)	71
Cfr13I	GGNCC	2 cut(s)	866, 928
Cfr42I	CCGCGG	1 cut(s)	104
Cfr9I	CCCGGG	2 cut(s)	659, 932
ClaI	ATCGAT	1 cut(s)	12
CseI	GACGC	2 cut(s)	1380, 1794
Csp6I	GTAC	2 cut(s)	815, 1602
CviAII	CATG	6 cut(s)	305, 358, 457, 673, 870, 1875
CviQI	GTAC	2 cut(s)	815, 1602
DdeI	CTNAG	6 cut(s)	120, 150, 639, 771, 879, 1587
DpnI	GATC	9 cut(s)	30, 189, 362, 533, 609, 677, 724, 1287, 1365
DpnII	GATC	9 cut(s)	28, 187, 360, 531, 607, 675, 722, 1285, 1363
Eam1104I	CTCTTC	4 cut(s)	150, 225, 732, 962
EarI	CTCTTC	4 cut(s)	150, 225, 732, 962
Ecl136II	GAGCTC	2 cut(s)	955, 1784
Eco130I	CCWWGG	2 cut(s)	625, 869
Eco147I	AGGCCT	2 cut(s)	790, 1324
Eco24I	GRGCYC	3 cut(s)	957, 1736, 1786
Eco31I	GGTCTC	3 cut(s)	80, 101, 206
Eco47I	GGWCC	1 cut(s)	928
Eco53kI	GAGCTC	2 cut(s)	955, 1784
Eco57I	CTGAAG	1 cut(s)	756
Eco88I	CYCGRG	2 cut(s)	659, 932
Eco91I	GGTNACC	2 cut(s)	242, 411
EcoICRI	GAGCTC	2 cut(s)	955, 1784
EcoO109I	RGGNCCY	1 cut(s)	928
EcoO65I	GGTNACC	2 cut(s)	242, 411
EcoRI	GAATTC	1 cut(s)	594
EcoRII	CCWGG	6 cut(s)	321, 790, 833, 1324, 1720, 1735
EcoT14I	CCWWGG	2 cut(s)	625, 869
EcoT22I	ATGCAT	1 cut(s)	897
EcoT38I	GRGCYC	3 cut(s)	957, 1736, 1786
ErhI	CCWWGG	2 cut(s)	625, 869
FaeI	CATG	6 cut(s)	308, 361, 460, 676, 873, 1878
FalI	AAGNNNNNCTT	6 cut(s)	801, 833, 1747, 1779, 1771, 1803
FaqI	GGGAC	3 cut(s)	185, 914, 1792
FatI	CATG	6 cut(s)	304, 357, 456, 672, 869, 1874
FauI	CCCGC	1 cut(s)	108
FbaI	TGATCA	4 cut(s)	28, 360, 607, 1363
Fnu4HI	GCNGC	5 cut(s)	104, 177, 842, 1247, 1577
FriOI	GRGCYC	3 cut(s)	957, 1736, 1786
Fsp4HI	GCNGC	5 cut(s)	104, 177, 842, 1247, 1577
FspBI	CTAG	4 cut(s)	921, 1251, 1292, 1437
GlaI	GCGC	3 cut(s)	280, 1796, 1804
GluI	GCNGC	5 cut(s)	104, 177, 842, 1247, 1577
GsaI	CCCAGC	1 cut(s)	1803
GsuI	CTGGAG	2 cut(s)	305, 856
HaeII	RGCGCY	1 cut(s)	1798
HaeIII	GGCC	5 cut(s)	50, 790, 867, 1324, 1471
HapII	CCGG	3 cut(s)	72, 660, 933
HgaI	GACGC	2 cut(s)	1380, 1794
HhaI	GCGC	3 cut(s)	281, 1797, 1805
Hin1I	GRCGYC	1 cut(s)	1391
Hin1II	CATG	6 cut(s)	308, 361, 460, 676, 873, 1878
Hin6I	GCGC	3 cut(s)	279, 1795, 1803
HinP1I	GCGC	3 cut(s)	279, 1795, 1803
HindIII	AAGCTT	3 cut(s)	1492, 1589, 1667
HinfI	GANTC	9 cut(s)	9, 85, 124, 134, 805, 1055, 1298, 1420, 1945
HpaII	CCGG	3 cut(s)	72, 660, 933
HphI	GGTGA	7 cut(s)	254, 641, 709, 1094, 1291, 1648, 1940
Hpy166II	GTNNAC	3 cut(s)	43, 317, 1342
Hpy188III	TCNNGA	6 cut(s)	58, 79, 535, 921, 1308, 1844
Hpy8I	GTNNAC	3 cut(s)	43, 317, 1342
Hpy99I	CGWCG	2 cut(s)	1107, 1566
HpyCH4III	ACNGT	2 cut(s)	209, 1518
HpyF10VI	GCNNNNNNNGC	6 cut(s)	146, 176, 379, 598, 1722, 1731
HpyF3I	CTNAG	6 cut(s)	120, 150, 639, 771, 879, 1587
Hsp92I	GRCGYC	1 cut(s)	1391
Hsp92II	CATG	6 cut(s)	308, 361, 460, 676, 873, 1878
HspAI	GCGC	3 cut(s)	279, 1795, 1803
KflI	GGGWCCC	1 cut(s)	928
Ksp22I	TGATCA	4 cut(s)	28, 360, 607, 1363
KspI	CCGCGG	1 cut(s)	104
Kzo9I	GATC	9 cut(s)	28, 187, 360, 531, 607, 675, 722, 1285, 1363
LguI	GCTCTTC	1 cut(s)	962
LmnI	GCTCC	3 cut(s)	288, 1573, 1789
Lsp1109I	GCAGC	2 cut(s)	163, 1563
LweI	GCATC	4 cut(s)	157, 1108, 1423, 1734
MaeI	CTAG	4 cut(s)	921, 1251, 1292, 1437
MaeIII	GTNAC	9 cut(s)	55, 110, 242, 411, 663, 802, 1424, 1654, 1946
MalI	GATC	9 cut(s)	30, 189, 362, 533, 609, 677, 724, 1287, 1365
MboI	GATC	9 cut(s)	28, 187, 360, 531, 607, 675, 722, 1285, 1363
MflI	RGATCY	2 cut(s)	531, 722
MhlI	GDGCHC	6 cut(s)	45, 319, 957, 1122, 1736, 1786
MlyI	GAGTC	2 cut(s)	799, 1954
Mph1103I	ATGCAT	1 cut(s)	897
MroXI	GAANNNNTTC	1 cut(s)	1839
MseI	TTAA	5 cut(s)	422, 999, 1047, 1466, 1551
MslI	CAYNNNNRTG	2 cut(s)	1349, 1431
MspA1I	CMGCKG	1 cut(s)	103
MspI	CCGG	3 cut(s)	72, 660, 933
Mva1269I	GAATGC	1 cut(s)	709
MvaI	CCWGG	6 cut(s)	323, 792, 835, 1326, 1722, 1737
MvnI	CGCG	3 cut(s)	103, 281, 1805
MwoI	GCNNNNNNNGC	6 cut(s)	146, 176, 379, 598, 1722, 1731
NciI	CCSGG	4 cut(s)	660, 661, 933, 934
NcoI	CCATGG	1 cut(s)	869
NdeII	GATC	9 cut(s)	28, 187, 360, 531, 607, 675, 722, 1285, 1363
NheI	GCTAGC	1 cut(s)	1436
NlaIII	CATG	6 cut(s)	308, 361, 460, 676, 873, 1878
NlaIV	GGNNCC	5 cut(s)	654, 929, 930, 1131, 1635
NmuCI	GTSAC	7 cut(s)	55, 242, 663, 802, 1424, 1654, 1946
NsiI	ATGCAT	1 cut(s)	897
NspV	TTCGAA	1 cut(s)	127
PceI	AGGCCT	2 cut(s)	790, 1324
PciSI	GCTCTTC	1 cut(s)	962
PctI	GAATGC	1 cut(s)	709
PdmI	GAANNNNTTC	1 cut(s)	1839
PfeI	GAWTC	7 cut(s)	9, 85, 124, 134, 1055, 1298, 1420
PflMI	CCANNNNNTGG	1 cut(s)	385
PfoI	TCCNGGA	1 cut(s)	321
PkrI	GCNGC	5 cut(s)	105, 178, 843, 1248, 1578
PleI	GAGTC	2 cut(s)	799, 1953
PpsI	GAGTC	2 cut(s)	799, 1953
PpuMI	RGGWCCY	1 cut(s)	928
Psp124BI	GAGCTC	2 cut(s)	957, 1786
Psp5II	RGGWCCY	1 cut(s)	928
Psp6I	CCWGG	6 cut(s)	321, 790, 833, 1324, 1720, 1735
PspEI	GGTNACC	2 cut(s)	242, 411
PspFI	CCCAGC	1 cut(s)	1799
PspGI	CCWGG	6 cut(s)	321, 790, 833, 1324, 1720, 1735
PspN4I	GGNNCC	5 cut(s)	654, 929, 930, 1131, 1635
PspPI	GGNCC	2 cut(s)	866, 928
PspPPI	RGGWCCY	1 cut(s)	928
PstNI	CAGNNNCTG	1 cut(s)	385
PsuI	RGATCY	2 cut(s)	531, 722
RsaI	GTAC	2 cut(s)	816, 1603
RsaNI	GTAC	2 cut(s)	815, 1602
RseI	CAYNNNNRTG	2 cut(s)	1349, 1431
SacI	GAGCTC	2 cut(s)	957, 1786
SacII	CCGCGG	1 cut(s)	104
SapI	GCTCTTC	1 cut(s)	962
SaqAI	TTAA	5 cut(s)	422, 999, 1047, 1466, 1551
SatI	GCNGC	5 cut(s)	104, 177, 842, 1247, 1577
Sau3AI	GATC	9 cut(s)	28, 187, 360, 531, 607, 675, 722, 1285, 1363
Sau96I	GGNCC	2 cut(s)	866, 928
ScaI	AGTACT	1 cut(s)	816
SchI	GAGTC	2 cut(s)	799, 1954
SduI	GDGCHC	6 cut(s)	45, 319, 957, 1122, 1736, 1786
SfaNI	GCATC	4 cut(s)	157, 1108, 1423, 1734
Sfr303I	CCGCGG	1 cut(s)	104
SfuI	TTCGAA	1 cut(s)	127
SgrBI	CCGCGG	1 cut(s)	104
SinI	GGWCC	1 cut(s)	928
SmaI	CCCGGG	2 cut(s)	661, 934
SmiMI	CAYNNNNRTG	2 cut(s)	1349, 1431
SmlI	CTYRAG	3 cut(s)	77, 726, 807
SmoI	CTYRAG	3 cut(s)	77, 726, 807
SseBI	AGGCCT	2 cut(s)	790, 1324
SsiI	CCGC	9 cut(s)	101, 103, 281, 436, 472, 841, 1247, 1401, 1706
SspMI	CTAG	4 cut(s)	921, 1251, 1292, 1437
SstI	GAGCTC	2 cut(s)	957, 1786
StuI	AGGCCT	2 cut(s)	790, 1324
StyI	CCWWGG	2 cut(s)	625, 869
TaaI	ACNGT	2 cut(s)	209, 1518
TaqI	TCGA	4 cut(s)	12, 127, 503, 1742
TatI	WGTACW	1 cut(s)	814
TauI	GCSGC	3 cut(s)	106, 844, 1249
TfiI	GAWTC	7 cut(s)	9, 85, 124, 134, 1055, 1298, 1420
Tru1I	TTAA	5 cut(s)	422, 999, 1047, 1466, 1551
Tru9I	TTAA	5 cut(s)	422, 999, 1047, 1466, 1551
TscAI	CASTG	3 cut(s)	390, 1635, 1778
TseFI	GTSAC	7 cut(s)	55, 242, 663, 802, 1424, 1654, 1946
TseI	GCWGC	2 cut(s)	176, 1576
Tsp45I	GTSAC	7 cut(s)	55, 242, 663, 802, 1424, 1654, 1946
TspDTI	ATGAA	9 cut(s)	132, 315, 487, 716, 929, 987, 993, 1400, 1749
TspGWI	ACGGA	1 cut(s)	1851
TspMI	CCCGGG	2 cut(s)	659, 932
TspRI	CASTG	3 cut(s)	390, 1635, 1778
Van91I	CCANNNNNTGG	1 cut(s)	385
VneI	GTGCAC	2 cut(s)	41, 315
VpaK11BI	GGWCC	1 cut(s)	928
XapI	RAATTY	2 cut(s)	550, 594
XbaI	TCTAGA	1 cut(s)	920
XmaI	CCCGGG	2 cut(s)	659, 932
XmnI	GAANNNNTTC	1 cut(s)	1839
XspI	CTAG	4 cut(s)	921, 1251, 1292, 1437
ZrmI	AGTACT	1 cut(s)	816
Zsp2I	ATGCAT	1 cut(s)	897

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.