Rh4DG002600

Ankyrin repeat domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0005480
Physical Location & Seq
Reverse (-)
494055 .. 501154
7100 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG002600.1

Sequence Viewer

Length: 1683 bp
ATGGCTGCAATTGATGTTCTCAAGTATGCACATAGTCCTGCACACAAAGCCATAGTCACCAAGGACTATGCCAGTCTCAGGAGGATTCTTGCTGCTCTTCCGCGGCTCTCTAATCCTGCCGAGATTCAAACTGAGTCAGCTTCATTAGCTGAAGAAGAGAAGGCCGATGCCATTGCTGCCGTCATTGATCGACGGGATGTCCCAAACCGTGACACCCCTCTTCACTTGGCTGTCAAACTTGGGGACCAGACAGCCACTGAAATGCTTATGGTTGCCGGGGCAGACTGGAGCTTGCAGAATGAGCAGGGGTGGAGTGCACTCCAGGAAGCAATTTGCAATAGAGAAGAAGCAATTTCCATGATTATAGTTAGGCACTATCAGCCATTGGCTTGGGCAAAATGGTGCAGAAGGTTGCCTCGTTTAATTGGAACTATGCGGAGGATGAGAGACTTCTACATGGAAATTACATTCCACTTCGAGAGTTCTGTGATTCCTTTCATTTCAAGGATTGCTCCATCAGACACATACAAGATCTGGAAAAGGGGTGCAAATTTGAGAGCAGACATGACATTGGCTGGTTTCGATGGTTTTAGGATTCAGCGTTCTGATCAGAGTGTTCTTTTTCTTGGTGATGGGTCTGACGATGGCAAGGTGCCTCCTGGGTCACTTTGCATGATCTCACACAAGGATAGGGAGGTGATGAATGCTTTGGATGGTGCTGGGTCTCAAGCAACTGAAGAAGAGGTTCGGCAAGAGGTGGTTGCAATGTCTCAGACTAATATATTCAGGCCTGGCATTGATGTGACTCAGGCAGTTCTCTTACCTCAGTTGACTTGGAGGCGGCAGGAGAAAACAGAAACGGTTGGATCATGGAAGGCTAAGGTATATGATATGCATAATGTGGTTGTGAGTATCAAGTCTAGGCAGGTCCCTGGGGCTATGACAGATGATGAGTTTTTCTCATCTTGCAATGAAAATGAAACAGAAAGTGAGGAACTCGATGATATTTTGACAGAGGATGAGAGGAGGCAACTTGAAGTTGCACTGAAACTGGATTCATCTGAATCGACCAACGAGAGTGGTGATGGAATTATTGAGCATCGTCATAGTTGTTATGAGGCAAGAGAAATTCCTGTTGAGGATGTAAGCAATTGTAGAAATGGAGAGACCAAGCAGGAAAAGAAAGGATGGTTTGGTGGATGGAGGAAACGAGATACCAAAAATGAAGGACATAAAAAGACTGTCCCACCAAGAAGTTCTCTTTGTGTGGATGAGAAGGTGAGTGATCTGCTAGGAGATTCTCCATCCAGAAATCAAAGCAGACCCAGAAGAAATAATGTGGACGCTGTTGTGAGGGGCGATGATCACAGGAGAGGAAGGGATATAAAAGCATCTTCCTCGCTGGGTTCTGATAGCAGAAGTCGTCATAAGGATGCAAGCAAGGAGAATGAGTATAAGAAAGGGCTAAGGCCTATTCTCTGGCTTTCCCCCAACTTTCCGCTACAAACAGAGGAACTGTTGCCGTTGCTTGATATTCTAGCAAACAAGGTTAAGGCCATCCGTCGTTTGAGGGAACTGCTTACTACCAAACTTCCAGTGGGAACATTTCCCGTCAAGTTTCTGAGTGGTTCCCATGTGTTTACATCTAGGTTGCTATCCCAGTGGTTCCCACTATCAGAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank_2 Ank_5 GPCR_chapero_1

Protein Analysis

560

Amino Acids

63.26

Weight (kDa)

6.54

Isoelectric Point (pI)

65.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ANKRD13_C PF11904 186 - 539 6.7e-96 ANKRD13 C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 916
AccB1I GGYRCC 1 cut(s) 652
AccII CGCG 1 cut(s) 103
AciI CCGC 5 cut(s) 101, 103, 436, 841, 1499
AclWI GGATC 1 cut(s) 874
AcsI RAATTY 2 cut(s) 550, 1128
AcuI CTGAAG 2 cut(s) 171, 756
AfiI CCNNNNNNNGG 1 cut(s) 78
AgsI TTSAA 3 cut(s) 128, 504, 1037
AhdI GACNNNNNGTC 1 cut(s) 197
AjnI CCWGG 4 cut(s) 321, 658, 790, 931
AloI GAACNNNNNNTCC 2 cut(s) 586, 618
AluBI AGCT 3 cut(s) 140, 149, 291
AluI AGCT 3 cut(s) 140, 149, 291
Alw21I GWGCWC 1 cut(s) 319
Alw26I GTCTC 5 cut(s) 80, 441, 729, 774, 1160
Alw44I GTGCAC 1 cut(s) 315
AlwI GGATC 1 cut(s) 874
AlwNI CAGNNNCTG 1 cut(s) 257
AoxI GGCC 4 cut(s) 162, 788, 1469, 1554
ApaLI GTGCAC 1 cut(s) 315
ApeKI GCWGC 3 cut(s) 5, 92, 176
ApoI RAATTY 2 cut(s) 550, 1128
ArsI GACNNNNNNTTYG 2 cut(s) 39, 71
Asp700I GAANNNNTTC 1 cut(s) 744
AspS9I GGNCC 2 cut(s) 244, 928
AsuC2I CCSGG 1 cut(s) 277
AsuHPI GGTGA 5 cut(s) 49, 641, 709, 1094, 1291
AvaII GGWCC 2 cut(s) 244, 928
BaeGI GKGCMC 1 cut(s) 319
BanI GGYRCC 1 cut(s) 652
Bbv12I GWGCWC 1 cut(s) 319
BbvI GCAGC 2 cut(s) 79, 163
BceAI ACGGC 2 cut(s) 164, 1507
BcgI CGANNNNNNTGC 4 cut(s) 155, 189, 1380, 1414
BciT130I CCWGG 4 cut(s) 323, 660, 792, 933
BclI TGATCA 2 cut(s) 607, 1363
BcnI CCSGG 1 cut(s) 277
BcoDI GTCTC 5 cut(s) 80, 441, 729, 774, 1160
BfaI CTAG 4 cut(s) 921, 1292, 1538, 1647
BfuAI ACCTGC 1 cut(s) 916
BglII AGATCT 1 cut(s) 531
BisI GCNGC 5 cut(s) 6, 93, 104, 177, 842
BlsI GCNGC 5 cut(s) 7, 94, 105, 178, 843
Bme1390I CCNGG 5 cut(s) 277, 323, 660, 792, 933
Bme18I GGWCC 2 cut(s) 244, 928
BmeRI GACNNNNNGTC 1 cut(s) 197
BmgT120I GGNCC 2 cut(s) 244, 928
BmiI GGNNCC 5 cut(s) 245, 654, 930, 1630, 1667
BmrFI CCNGG 5 cut(s) 277, 323, 660, 792, 933
BmrI ACTGGG 1 cut(s) 1654
BmsI GCATC 4 cut(s) 157, 1108, 1400, 1423
BmuI ACTGGG 1 cut(s) 1654
BplI GAGNNNNNCTC 2 cut(s) 944, 976
BpmI CTGGAG 2 cut(s) 305, 307
Bpu10I CCTNAGC 2 cut(s) 879, 1466
BpuEI CTTGAG 2 cut(s) 5, 711
BpuMI CCSGG 1 cut(s) 277
BsaI GGTCTC 2 cut(s) 729, 1160
BsaJI CCNNGG 6 cut(s) 60, 101, 276, 659, 931, 932
BsaXI ACNNNNNCTCC 2 cut(s) 1436, 1466
Bsc4I CCNNNNNNNGG 1 cut(s) 78
Bse1I ACTGG 5 cut(s) 72, 290, 1056, 1595, 1660
Bse3DI GCAATG 3 cut(s) 171, 771, 976
BseBI CCWGG 4 cut(s) 323, 660, 792, 933
BseDI CCNNGG 6 cut(s) 60, 101, 276, 659, 931, 932
BseLI CCNNNNNNNGG 1 cut(s) 78
BseMI GCAATG 3 cut(s) 171, 771, 976
BseMII CTCAG 6 cut(s) 91, 123, 785, 821, 839, 1613
BseNI ACTGG 5 cut(s) 72, 290, 1056, 1595, 1660
BseRI GAGGAG 1 cut(s) 1039
BseSI GKGCMC 1 cut(s) 319
BseXI GCAGC 2 cut(s) 79, 163
BseYI CCCAGC 2 cut(s) 719, 1402
BsgI GTGCAG 2 cut(s) 24, 424
Bsh1236I CGCG 1 cut(s) 103
BshFI GGCC 4 cut(s) 164, 790, 1471, 1556
BshNI GGYRCC 1 cut(s) 652
BsiHKAI GWGCWC 1 cut(s) 319
BsiSI CCGG 1 cut(s) 276
BslFI GGGAC 4 cut(s) 185, 257, 914, 1229
BslI CCNNNNNNNGG 1 cut(s) 78
BsmAI GTCTC 5 cut(s) 80, 441, 729, 774, 1160
BsmFI GGGAC 4 cut(s) 185, 257, 914, 1229
BsmI GAATGC 1 cut(s) 709
BsnI GGCC 4 cut(s) 164, 790, 1471, 1556
Bso31I GGTCTC 2 cut(s) 729, 1160
Bsp1286I GDGCHC 1 cut(s) 319
Bsp143I GATC 7 cut(s) 187, 531, 607, 675, 866, 1285, 1363
BspACI CCGC 5 cut(s) 101, 103, 436, 841, 1499
BspANI GGCC 4 cut(s) 164, 790, 1471, 1556
BspCNI CTCAG 6 cut(s) 90, 124, 784, 820, 838, 1614
BspFNI CGCG 1 cut(s) 103
BspLI GGNNCC 5 cut(s) 245, 654, 930, 1630, 1667
BspMI ACCTGC 1 cut(s) 916
BspPI GGATC 1 cut(s) 874
BspQI GCTCTTC 1 cut(s) 102
BspT107I GGYRCC 1 cut(s) 652
BspTNI GGTCTC 2 cut(s) 729, 1160
BsrDI GCAATG 3 cut(s) 171, 771, 976
BsrI ACTGG 5 cut(s) 72, 290, 1056, 1595, 1660
BssECI CCNNGG 6 cut(s) 60, 101, 276, 659, 931, 932
BssMI GATC 7 cut(s) 187, 531, 607, 675, 866, 1285, 1363
BssT1I CCWWGG 1 cut(s) 60
Bst2UI CCWGG 4 cut(s) 323, 660, 792, 933
Bst4CI ACNGT 4 cut(s) 209, 862, 1243, 1518
Bst6I CTCTTC 4 cut(s) 102, 150, 225, 735
BstC8I GCNNGC 2 cut(s) 293, 1438
BstDEI CTNAG 8 cut(s) 77, 132, 771, 807, 825, 879, 1466, 1622
BstDSI CCRYGG 1 cut(s) 101
BstFNI CGCG 1 cut(s) 103
BstKTI GATC 7 cut(s) 190, 534, 610, 678, 869, 1288, 1366
BstMAI GTCTC 5 cut(s) 80, 441, 729, 774, 1160
BstMBI GATC 7 cut(s) 187, 531, 607, 675, 866, 1285, 1363
BstMWI GCNNNNNNNGC 5 cut(s) 47, 146, 176, 301, 379
BstNI CCWGG 4 cut(s) 323, 660, 792, 933
BstSCI CCNGG 5 cut(s) 275, 321, 658, 790, 931
BstSLI GKGCMC 1 cut(s) 319
BstUI CGCG 1 cut(s) 103
BstV1I GCAGC 2 cut(s) 79, 163
BstX2I RGATCY 1 cut(s) 531
BstXI CCANNNNNNTGG 1 cut(s) 390
BstYI RGATCY 1 cut(s) 531
BsuRI GGCC 4 cut(s) 164, 790, 1471, 1556
BtgI CCRYGG 1 cut(s) 101
BtgZI GCGATG 1 cut(s) 1374
BtsIMutI CAGTG 4 cut(s) 255, 1043, 1602, 1667
BveI ACCTGC 1 cut(s) 916
Cac8I GCNNGC 2 cut(s) 293, 1438
CaiI CAGNNNCTG 1 cut(s) 257
Cfr13I GGNCC 2 cut(s) 244, 928
Cfr42I CCGCGG 1 cut(s) 104
CseI GACGC 1 cut(s) 1352
CspCI CAANNNNNGTGG 2 cut(s) 1060, 1095
CviAII CATG 6 cut(s) 358, 457, 565, 673, 870, 1634
DdeI CTNAG 8 cut(s) 77, 132, 771, 807, 825, 879, 1466, 1622
DpnI GATC 7 cut(s) 189, 533, 609, 677, 868, 1287, 1365
DpnII GATC 7 cut(s) 187, 531, 607, 675, 866, 1285, 1363
DriI GACNNNNNGTC 1 cut(s) 197
Eam1104I CTCTTC 4 cut(s) 102, 150, 225, 735
Eam1105I GACNNNNNGTC 1 cut(s) 197
EarI CTCTTC 4 cut(s) 102, 150, 225, 735
Eco130I CCWWGG 1 cut(s) 60
Eco147I AGGCCT 2 cut(s) 790, 1471
Eco31I GGTCTC 2 cut(s) 729, 1160
Eco47I GGWCC 2 cut(s) 244, 928
Eco57I CTGAAG 2 cut(s) 171, 756
EcoO109I RGGNCCY 1 cut(s) 928
EcoRII CCWGG 4 cut(s) 321, 658, 790, 931
EcoT14I CCWWGG 1 cut(s) 60
EcoT22I ATGCAT 1 cut(s) 897
ErhI CCWWGG 1 cut(s) 60
FaeI CATG 6 cut(s) 361, 460, 568, 676, 873, 1637
FaqI GGGAC 4 cut(s) 185, 257, 914, 1229
FatI CATG 6 cut(s) 357, 456, 564, 672, 869, 1633
FbaI TGATCA 2 cut(s) 607, 1363
Fnu4HI GCNGC 5 cut(s) 6, 93, 104, 177, 842
Fsp4HI GCNGC 5 cut(s) 6, 93, 104, 177, 842
FspBI CTAG 4 cut(s) 921, 1292, 1538, 1647
GluI GCNGC 5 cut(s) 6, 93, 104, 177, 842
GsaI CCCAGC 2 cut(s) 723, 1406
GsuI CTGGAG 2 cut(s) 305, 307
HaeIII GGCC 4 cut(s) 164, 790, 1471, 1556
HapII CCGG 1 cut(s) 276
HgaI GACGC 1 cut(s) 1352
Hin1II CATG 6 cut(s) 361, 460, 568, 676, 873, 1637
HincII GTYRAC 1 cut(s) 831
HindII GTYRAC 1 cut(s) 831
HinfI GANTC 9 cut(s) 85, 124, 134, 490, 595, 805, 1055, 1064, 1298
HpaII CCGG 1 cut(s) 276
HphI GGTGA 5 cut(s) 49, 641, 709, 1094, 1291
Hpy166II GTNNAC 4 cut(s) 317, 831, 1342, 1641
Hpy188I TCNGA 9 cut(s) 520, 607, 612, 640, 774, 1063, 1411, 1623, 1678
Hpy188III TCNNGA 4 cut(s) 79, 478, 535, 1308
Hpy8I GTNNAC 4 cut(s) 317, 831, 1342, 1641
Hpy99I CGWCG 2 cut(s) 195, 1566
HpyAV CCTTC 6 cut(s) 154, 402, 868, 1220, 1270, 1371
HpyCH4III ACNGT 4 cut(s) 209, 862, 1243, 1518
HpyF10VI GCNNNNNNNGC 5 cut(s) 47, 146, 176, 301, 379
HpyF3I CTNAG 8 cut(s) 77, 132, 771, 807, 825, 879, 1466, 1622
Hsp92II CATG 6 cut(s) 361, 460, 568, 676, 873, 1637
Ksp22I TGATCA 2 cut(s) 607, 1363
KspI CCGCGG 1 cut(s) 104
Kzo9I GATC 7 cut(s) 187, 531, 607, 675, 866, 1285, 1363
LguI GCTCTTC 1 cut(s) 102
LmnI GCTCC 2 cut(s) 288, 517
Lsp1109I GCAGC 2 cut(s) 79, 163
LweI GCATC 4 cut(s) 157, 1108, 1400, 1423
MaeI CTAG 4 cut(s) 921, 1292, 1538, 1647
MaeIII GTNAC 4 cut(s) 55, 209, 663, 802
MalI GATC 7 cut(s) 189, 533, 609, 677, 868, 1287, 1365
MboI GATC 7 cut(s) 187, 531, 607, 675, 866, 1285, 1363
MboII GAAGA 9 cut(s) 89, 164, 167, 212, 356, 749, 752, 1341, 1386
MfeI CAATTG 2 cut(s) 9, 1150
MflI RGATCY 1 cut(s) 531
MhlI GDGCHC 1 cut(s) 319
MluCI AATT 9 cut(s) 9, 330, 351, 423, 462, 550, 1089, 1128, 1150
MlyI GAGTC 2 cut(s) 143, 799
MmeI TCCRAC 1 cut(s) 844
Mph1103I ATGCAT 1 cut(s) 897
MroXI GAANNNNTTC 1 cut(s) 744
MseI TTAA 2 cut(s) 422, 1551
MslI CAYNNNNRTG 3 cut(s) 260, 800, 1431
MspA1I CMGCKG 1 cut(s) 103
MspI CCGG 1 cut(s) 276
MspR9I CCNGG 5 cut(s) 277, 323, 660, 792, 933
MunI CAATTG 2 cut(s) 9, 1150
Mva1269I GAATGC 1 cut(s) 709
MvaI CCWGG 4 cut(s) 323, 660, 792, 933
MvnI CGCG 1 cut(s) 103
MwoI GCNNNNNNNGC 5 cut(s) 47, 146, 176, 301, 379
NciI CCSGG 1 cut(s) 277
NdeII GATC 7 cut(s) 187, 531, 607, 675, 866, 1285, 1363
NlaIII CATG 6 cut(s) 361, 460, 568, 676, 873, 1637
NlaIV GGNNCC 5 cut(s) 245, 654, 930, 1630, 1667
NmeAIII GCCGAG 1 cut(s) 145
NmuCI GTSAC 4 cut(s) 55, 209, 663, 802
NsiI ATGCAT 1 cut(s) 897
PasI CCCWGGG 1 cut(s) 932
PceI AGGCCT 2 cut(s) 790, 1471
PciSI GCTCTTC 1 cut(s) 102
PctI GAATGC 1 cut(s) 709
PdmI GAANNNNTTC 1 cut(s) 744
PfeI GAWTC 7 cut(s) 85, 124, 490, 595, 1055, 1064, 1298
PfoI TCCNGGA 1 cut(s) 321
PkrI GCNGC 5 cut(s) 7, 94, 105, 178, 843
PleI GAGTC 2 cut(s) 142, 799
PpsI GAGTC 2 cut(s) 142, 799
PpuMI RGGWCCY 1 cut(s) 928
Psp5II RGGWCCY 1 cut(s) 928
Psp6I CCWGG 4 cut(s) 321, 658, 790, 931
PspFI CCCAGC 2 cut(s) 719, 1402
PspGI CCWGG 4 cut(s) 321, 658, 790, 931
PspN4I GGNNCC 5 cut(s) 245, 654, 930, 1630, 1667
PspPI GGNCC 2 cut(s) 244, 928
PspPPI RGGWCCY 1 cut(s) 928
PstNI CAGNNNCTG 1 cut(s) 257
PsuI RGATCY 1 cut(s) 531
RseI CAYNNNNRTG 3 cut(s) 260, 800, 1431
SacII CCGCGG 1 cut(s) 104
SapI GCTCTTC 1 cut(s) 102
SaqAI TTAA 2 cut(s) 422, 1551
SatI GCNGC 5 cut(s) 6, 93, 104, 177, 842
Sau3AI GATC 7 cut(s) 187, 531, 607, 675, 866, 1285, 1363
Sau96I GGNCC 2 cut(s) 244, 928
SchI GAGTC 2 cut(s) 143, 799
ScrFI CCNGG 5 cut(s) 277, 323, 660, 792, 933
SduI GDGCHC 1 cut(s) 319
SfaNI GCATC 4 cut(s) 157, 1108, 1400, 1423
Sfr303I CCGCGG 1 cut(s) 104
SgrBI CCGCGG 1 cut(s) 104
SinI GGWCC 2 cut(s) 244, 928
SmiMI CAYNNNNRTG 3 cut(s) 260, 800, 1431
SmlI CTYRAG 2 cut(s) 20, 726
SmoI CTYRAG 2 cut(s) 20, 726
Sse9I AATT 9 cut(s) 9, 330, 351, 423, 462, 550, 1089, 1128, 1150
SseBI AGGCCT 2 cut(s) 790, 1471
SsiI CCGC 5 cut(s) 101, 103, 436, 841, 1499
SspMI CTAG 4 cut(s) 921, 1292, 1538, 1647
StuI AGGCCT 2 cut(s) 790, 1471
StyD4I CCNGG 5 cut(s) 275, 321, 658, 790, 931
StyI CCWWGG 1 cut(s) 60
TaaI ACNGT 4 cut(s) 209, 862, 1243, 1518
TaqI TCGA 5 cut(s) 190, 477, 582, 999, 1067
TasI AATT 9 cut(s) 9, 330, 351, 423, 462, 550, 1089, 1128, 1150
TauI GCSGC 2 cut(s) 106, 844
TfiI GAWTC 7 cut(s) 85, 124, 490, 595, 1055, 1064, 1298
Tru1I TTAA 2 cut(s) 422, 1551
Tru9I TTAA 2 cut(s) 422, 1551
TscAI CASTG 4 cut(s) 262, 1050, 1602, 1667
TseFI GTSAC 4 cut(s) 55, 209, 663, 802
TseI GCWGC 3 cut(s) 5, 92, 176
Tsp45I GTSAC 4 cut(s) 55, 209, 663, 802
TspDTI ATGAA 7 cut(s) 132, 487, 716, 987, 993, 1047, 1239
TspGWI ACGGA 1 cut(s) 1550
TspRI CASTG 4 cut(s) 262, 1050, 1602, 1667
VneI GTGCAC 1 cut(s) 315
VpaK11BI GGWCC 2 cut(s) 244, 928
XapI RAATTY 2 cut(s) 550, 1128
XcmI CCANNNNNNNNNTGG 1 cut(s) 1594
XmnI GAANNNNTTC 1 cut(s) 744
XspI CTAG 4 cut(s) 921, 1292, 1538, 1647
Zsp2I ATGCAT 1 cut(s) 897
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.