Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr4

Orthogroup ID

OG0005480

Physical Location & Seq

Genomic Coordinates Reverse (-)

291349 .. 295687

Gene Structure 4339 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

pycom04g00300.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2058 bp

ATGGCTGGAATCGATGTGTCCAAGTATGATCATAGCCCCGTGCACAAGGCCATAGTCACGAGGGATTATGCCGGTCTCAAGAGGATTCTATCTGGTCTCCCGCGGCTTTGTAACCCTGCTGAGATTCGAACTGAATCAGCTTCATTAGCTGAGGAAGAGAAGGCTGATGCCATTGCTGCTGTCATTGATCGGCGAGATGTCCCAAACCGTGAGACCCCCCTTCACTTGGCTGTAAAACTTGGTGACCAGACAGCAACCGAAATGCTTATGGTTGCCGGCGCGGATTGGAGCTTGCAGAATGAACATGGATGGAGTGCACTCCAGGAAGCAATTTGCAATAGAGAAGAAGCAATTTCAATGATCATAGTTAGGCACTACCAGCCACTGGCTTGGGCGAAATGGTGCAGAAGGTTACCTCGTTTAATTGGAACAATGCGGAGGATGAGGGATTTCTACATGGAAATTACATTCCGCTTTGAGAGTTCTGTTATACCTTTCATTTCGAGGATTGCCCCATCAGATACTTACAAGATCTGGAAAAAGGGTGCAAATTTGAGAGCAGATATGACATTGGCAGGTTTTGATGGTTTGCGAATTCAGCGTTCTGATCAGAGTGTTCTTTTCCTCGGTGATGGGTCTGAAGATGGTAAGGTGCCTCCTGGGTCACTTTGCATGATCTCACACAAGGATAAGGAGGTGATGAATGCTTTGGATGGTGCTGGATCCCAAGCAACTGAAGAGGAGGTTCGGCAAGAGGTGGTTGCAATGTCTCAGACTAATATATTCAGGCCTGGGGTTGATGTGACTCAAGCAGTACTTTTACCTCAAATGACCTGGAGGCGGCAGGAGAAAACAGAAATGGTTGGCCCATGGAAGGCTAAGGTATATGATATGCATAATGTGGTTGTGAGTATGAAATCTAGAAGGGTCCCCGGGGCTATGACAGATGACGAGCTCTTCTCAACCTGCAATGAAAATGAAACAGAAAGTGAGGAGTTTAATGATATTCTGACAGAGGATGAGAGGAAGCAACTTGAAAATGCACTTAAATTGGATTCGTCGGACTTGTCAAACGAAAATGGTGATGGGATTATTGAGCATCGTCGTAGTTGTTATGAGCACAGGGAGGTTCCCATTGAAAATGTTAGTAGTGATAGAAATGGAGAGAATAAGCAGGAAAAGAAAGGATGGTTTGGTGGATGGAGGAAACGGGATACTAAAAGTGAAGGGCAGAAAAAGATTGTGCCGCCTAGAAGTTCTCTCTGTGTAGATGAGAAGGTGAGTGATCTGCTAGGGGATTCTCCTTCAAGAAATCAAAGCAGACCTGGAAGAAATAGTGTGGACATTGTTGTGAAGGGGGATGATCATCGGAGAGGAAGGGATATGAAGGCGTCTTCTTCCACAAGTTCTGATAACAGGAATCGTCACAAGGATGCTAGCAAGGAGAATGAGTATAAGAAAGGATTAAGGCCAATCCTCTGGCTTTCCCCAAGCTTTCCATTACAAACAGAGGAACTGTTGCCATTACTTGATATTTTAGCAAACAAGGTTAAGGCAATTCGTCGTTTGAGGGAGCTGCTTACAACTAAGCTTCCAATGGGTACATTTCCAGTCAAGGCACCTTGGAAACATGGTGATGTTTTAACCTACTTGGTTAACCTAACCGAGGAGAATGTGTCAACTTTTGAAGAAATGGGGAGTCGCATTGTAGTTATTGTTGCTATCCCAGTGGTTCCTACTATCAGAGTAATTGTCACCTTCACAAAGCTTGAAGAATTACCACCAATGGATGAGTTTGCAACACCACCATCAAGCCCCCTTGGCAGGGAGAGCCCAAGTTCGATGCAATCGTCAAGTTCATCTTGGTTTCAGTGGATAAGAGCTCCTTACCAGCGCCCTAGCATGTCCCCTACTGGTTCTTTCAGTAAGATAGAAAGTATTCAAGACCCGTTTGCAATACCGTCAGAATATACATGGGTTTCTGTGGAAGCAAAGAAAAAGAAAATGCAGGAGAAGAACAAGTCAAAGAAAGGGAAAAGTCAGAGTCACCAAAAATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Ank_2 Ank_5 GPCR_chapero_1

Protein Analysis

686

Amino Acids

77.43

Weight (kDa)

8.25

Isoelectric Point (pI)

64.5

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
ANKRD13_C	PF11904	186 - 539	2.8e-97	ANKRD13 C-terminal
ANKRD13_C	PF11904	572 - 658	6.6e-11	ANKRD13 C-terminal

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0005480)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G04780
fragaria_vesca	FvH4_4g00280 FvH4_4g00280 FvH4_4g00280 FvH4_4g00280
malus_domestica	MD04G1002900.v1.1 MD13G1285000.v1.1
prunus_persica	Prupe.1G002800_v2.0.a1 Prupe.1G002800_v2.0.a1 Prupe.1G002800_v2.0.a1 Prupe.1G002800_v2.0.a1 Prupe.1G002800_v2.0.a1
pyrus_communis	pycom04g00300 pycom420g00660
rosa_chinensis	RchiOBHm_Chr4g0385121
rosa_laevigata	RLG00000010209 RLG00000010228 RLG00000010256
rosa_multiflora	Rmu_ssc0000158.1_g000038
rosa_roxburghii	Rroxscaffold_5G00333030 Rroxscaffold_5G00364810
rosa_rugosa	Rorug03G0299200 Rorug03G0299300 Rorug03G0299300
rosa_samantha	Rh4AG002800 Rh4BG002300 Rh4CG003100 Rh4DG002600
rosa_wichuraiana	Rw4G000650

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	2 cut(s)	566, 974
AccB1I	GGYRCC	2 cut(s)	652, 1618
AccB7I	CCANNNNNTGG	1 cut(s)	385
AccII	CGCG	2 cut(s)	103, 281
AciI	CCGC	7 cut(s)	101, 103, 281, 436, 472, 841, 1247
AclWI	GGATC	2 cut(s)	717, 730
AcsI	RAATTY	2 cut(s)	550, 594
AcuI	CTGAAG	2 cut(s)	660, 756
AcyI	GRCGYC	1 cut(s)	1391
AfaI	GTAC	2 cut(s)	816, 1603
AfiI	CCNNNNNNNGG	7 cut(s)	226, 385, 840, 874, 1666, 1824, 1825
AgsI	TTSAA	7 cut(s)	357, 1037, 1139, 1308, 1688, 1772, 1943
AjnI	CCWGG	5 cut(s)	321, 658, 790, 833, 1324
AluBI	AGCT	9 cut(s)	140, 149, 291, 955, 1494, 1576, 1591, 1768, 1883
AluI	AGCT	9 cut(s)	140, 149, 291, 955, 1494, 1576, 1591, 1768, 1883
Alw21I	GWGCWC	5 cut(s)	45, 319, 957, 1122, 1885
Alw26I	GTCTC	4 cut(s)	80, 101, 206, 774
Alw44I	GTGCAC	2 cut(s)	41, 315
AlwI	GGATC	2 cut(s)	717, 730
AlwNI	CAGNNNCTG	1 cut(s)	385
Ama87I	CYCGRG	1 cut(s)	932
AoxI	GGCC	4 cut(s)	48, 788, 865, 1469
ApaLI	GTGCAC	2 cut(s)	41, 315
ApeKI	GCWGC	2 cut(s)	176, 1576
ApoI	RAATTY	2 cut(s)	550, 594
Asp700I	GAANNNNTTC	1 cut(s)	1938
AspLEI	GCGC	2 cut(s)	281, 1896
AspS9I	GGNCC	2 cut(s)	866, 928
AsuC2I	CCSGG	2 cut(s)	933, 934
AsuHPI	GGTGA	8 cut(s)	254, 641, 709, 1094, 1291, 1646, 1747, 2039
AsuII	TTCGAA	1 cut(s)	127
AsuNHI	GCTAGC	1 cut(s)	1436
AvaI	CYCGRG	1 cut(s)	932
AvaII	GGWCC	1 cut(s)	928
BaeGI	GKGCMC	2 cut(s)	45, 319
BamHI	GGATCC	1 cut(s)	722
BanI	GGYRCC	2 cut(s)	652, 1618
BanII	GRGCYC	3 cut(s)	957, 1835, 1885
BauI	CACGAG	1 cut(s)	58
BbsI	GAAGAC	1 cut(s)	1386
Bbv12I	GWGCWC	5 cut(s)	45, 319, 957, 1122, 1885
BbvCI	CCTCAGC	1 cut(s)	150
BbvI	GCAGC	2 cut(s)	163, 1563
BciT130I	CCWGG	5 cut(s)	323, 660, 792, 835, 1326
BciVI	GTATCC	1 cut(s)	1207
BclI	TGATCA	4 cut(s)	28, 360, 607, 1363
BcnI	CCSGG	2 cut(s)	933, 934
BcoDI	GTCTC	4 cut(s)	80, 101, 206, 774
BfaI	CTAG	5 cut(s)	921, 1251, 1292, 1437, 1899
BfoI	RGCGCY	1 cut(s)	1897
BfuAI	ACCTGC	2 cut(s)	566, 974
BfuI	GTATCC	1 cut(s)	1207
BglI	GCCNNNNNGGC	1 cut(s)	1821
BglII	AGATCT	1 cut(s)	531
BisI	GCNGC	5 cut(s)	104, 177, 842, 1247, 1577
BlsI	GCNGC	5 cut(s)	105, 178, 843, 1248, 1578
BmcAI	AGTACT	1 cut(s)	816
Bme1390I	CCNGG	7 cut(s)	323, 660, 792, 835, 933, 934, 1326
Bme18I	GGWCC	1 cut(s)	928
BmeT110I	CYCGRG	1 cut(s)	932
BmgT120I	GGNCC	2 cut(s)	866, 928
BmiI	GGNNCC	7 cut(s)	654, 724, 929, 930, 1131, 1620, 1734
BmrFI	CCNGG	7 cut(s)	323, 660, 792, 835, 933, 934, 1326
BmrI	ACTGGG	1 cut(s)	1721
BmsI	GCATC	4 cut(s)	157, 1108, 1423, 1833
BmtI	GCTAGC	1 cut(s)	1440
BmuI	ACTGGG	1 cut(s)	1721
BpiI	GAAGAC	1 cut(s)	1386
BplI	GAGNNNNNCTC	2 cut(s)	944, 976
BpmI	CTGGAG	2 cut(s)	305, 856
Bpu10I	CCTNAGC	2 cut(s)	150, 879
Bpu14I	TTCGAA	1 cut(s)	127
BpuEI	CTTGAG	2 cut(s)	62, 792
BpuMI	CCSGG	2 cut(s)	933, 934
Bsa29I	ATCGAT	1 cut(s)	12
BsaBI	GATNNNNATC	1 cut(s)	1365
BsaHI	GRCGYC	1 cut(s)	1391
BsaI	GGTCTC	3 cut(s)	80, 101, 206
BsaXI	ACNNNNNCTCC	4 cut(s)	1436, 1466, 1661, 1691
Bsc4I	CCNNNNNNNGG	7 cut(s)	226, 385, 840, 874, 1666, 1824, 1825
Bse118I	RCCGGY	2 cut(s)	71, 275
Bse1I	ACTGG	4 cut(s)	390, 1610, 1727, 1918
Bse3DI	GCAATG	3 cut(s)	171, 771, 976
Bse8I	GATNNNNATC	1 cut(s)	1365
BseBI	CCWGG	5 cut(s)	323, 660, 792, 835, 1326
BseCI	ATCGAT	1 cut(s)	12
BseGI	GGATG	9 cut(s)	314, 447, 718, 1024, 1193, 1205, 1366, 1438, 1795
BseJI	GATNNNNATC	1 cut(s)	1365
BseLI	CCNNNNNNNGG	7 cut(s)	226, 385, 840, 874, 1666, 1824, 1825
BseMI	GCAATG	3 cut(s)	171, 771, 976
BseMII	CTCAG	3 cut(s)	111, 141, 785
BseNI	ACTGG	4 cut(s)	390, 1610, 1727, 1918
BseRI	GAGGAG	3 cut(s)	755, 1007, 1682
BseSI	GKGCMC	2 cut(s)	45, 319
BseXI	GCAGC	2 cut(s)	163, 1563
BsgI	GTGCAG	1 cut(s)	424
Bsh1236I	CGCG	2 cut(s)	103, 281
BshFI	GGCC	4 cut(s)	50, 790, 867, 1471
BshNI	GGYRCC	2 cut(s)	652, 1618
BshVI	ATCGAT	1 cut(s)	12
BsiHKAI	GWGCWC	5 cut(s)	45, 319, 957, 1122, 1885
BsiHKCI	CYCGRG	1 cut(s)	932
BsiSI	CCGG	3 cut(s)	72, 276, 933
BslFI	GGGAC	3 cut(s)	185, 914, 1891
BslI	CCNNNNNNNGG	7 cut(s)	226, 385, 840, 874, 1666, 1824, 1825
BsmAI	GTCTC	4 cut(s)	80, 101, 206, 774
BsmFI	GGGAC	3 cut(s)	185, 914, 1891
BsmI	GAATGC	1 cut(s)	709
BsnI	GGCC	4 cut(s)	50, 790, 867, 1471
Bso31I	GGTCTC	3 cut(s)	80, 101, 206
BsoBI	CYCGRG	1 cut(s)	932
Bsp119I	TTCGAA	1 cut(s)	127
Bsp1286I	GDGCHC	6 cut(s)	45, 319, 957, 1122, 1835, 1885
Bsp143I	GATC	9 cut(s)	28, 187, 360, 531, 607, 675, 722, 1285, 1363
Bsp19I	CCATGG	1 cut(s)	869
BspACI	CCGC	7 cut(s)	101, 103, 281, 436, 472, 841, 1247
BspANI	GGCC	4 cut(s)	50, 790, 867, 1471
BspCNI	CTCAG	3 cut(s)	112, 142, 784
BspDI	ATCGAT	1 cut(s)	12
BspFNI	CGCG	2 cut(s)	103, 281
BspLI	GGNNCC	7 cut(s)	654, 724, 929, 930, 1131, 1620, 1734
BspMI	ACCTGC	2 cut(s)	566, 974
BspOI	GCTAGC	1 cut(s)	1440
BspPI	GGATC	2 cut(s)	717, 730
BspQI	GCTCTTC	1 cut(s)	962
BspT104I	TTCGAA	1 cut(s)	127
BspT107I	GGYRCC	2 cut(s)	652, 1618
BspTNI	GGTCTC	3 cut(s)	80, 101, 206
BsrDI	GCAATG	3 cut(s)	171, 771, 976
BsrFI	RCCGGY	2 cut(s)	71, 275
BsrI	ACTGG	4 cut(s)	390, 1610, 1727, 1918
BssAI	RCCGGY	2 cut(s)	71, 275
BssMI	GATC	9 cut(s)	28, 187, 360, 531, 607, 675, 722, 1285, 1363
BssNI	GRCGYC	1 cut(s)	1391
BssSI	CACGAG	1 cut(s)	58
BssT1I	CCWWGG	3 cut(s)	869, 1622, 1819
Bst2BI	CACGAG	1 cut(s)	58
Bst2UI	CCWGG	5 cut(s)	323, 660, 792, 835, 1326
Bst4CI	ACNGT	3 cut(s)	209, 1518, 1962
Bst6I	CTCTTC	3 cut(s)	150, 732, 962
BstACI	GRCGYC	1 cut(s)	1391
BstBI	TTCGAA	1 cut(s)	127
BstC8I	GCNNGC	3 cut(s)	277, 293, 1438
BstDEI	CTNAG	5 cut(s)	120, 150, 771, 879, 1587
BstDSI	CCRYGG	2 cut(s)	101, 869
BstEII	GGTNACC	2 cut(s)	242, 411
BstENI	CCTNNNNNAGG	1 cut(s)	1664
BstF5I	GGATG	9 cut(s)	314, 447, 718, 1024, 1193, 1205, 1366, 1438, 1795
BstFNI	CGCG	2 cut(s)	103, 281
BstH2I	RGCGCY	1 cut(s)	1897
BstHHI	GCGC	2 cut(s)	281, 1896
BstKTI	GATC	9 cut(s)	31, 190, 363, 534, 610, 678, 725, 1288, 1366
BstMAI	GTCTC	4 cut(s)	80, 101, 206, 774
BstMBI	GATC	9 cut(s)	28, 187, 360, 531, 607, 675, 722, 1285, 1363
BstMWI	GCNNNNNNNGC	6 cut(s)	146, 176, 379, 598, 1821, 1830
BstNI	CCWGG	5 cut(s)	323, 660, 792, 835, 1326
BstNSI	RCATGY	1 cut(s)	1906
BstPI	GGTNACC	2 cut(s)	242, 411
BstSCI	CCNGG	7 cut(s)	321, 658, 790, 833, 931, 932, 1324
BstSLI	GKGCMC	2 cut(s)	45, 319
BstUI	CGCG	2 cut(s)	103, 281
BstV1I	GCAGC	2 cut(s)	163, 1563
BstV2I	GAAGAC	1 cut(s)	1386
BstX2I	RGATCY	2 cut(s)	531, 722
BstXI	CCANNNNNNTGG	2 cut(s)	390, 1479
BstYI	RGATCY	2 cut(s)	531, 722
Bsu15I	ATCGAT	1 cut(s)	12
BsuI	GTATCC	1 cut(s)	1207
BsuRI	GGCC	4 cut(s)	50, 790, 867, 1471
BsuTUI	ATCGAT	1 cut(s)	12
BtgI	CCRYGG	2 cut(s)	101, 869
BtsCI	GGATG	9 cut(s)	314, 447, 718, 1024, 1193, 1205, 1366, 1438, 1795
BtsIMutI	CAGTG	3 cut(s)	383, 1734, 1877
BveI	ACCTGC	2 cut(s)	566, 974
Cac8I	GCNNGC	3 cut(s)	277, 293, 1438
CaiI	CAGNNNCTG	1 cut(s)	385
CfoI	GCGC	2 cut(s)	281, 1896
Cfr10I	RCCGGY	2 cut(s)	71, 275
Cfr13I	GGNCC	2 cut(s)	866, 928
Cfr42I	CCGCGG	1 cut(s)	104
Cfr9I	CCCGGG	1 cut(s)	932
ClaI	ATCGAT	1 cut(s)	12
CseI	GACGC	1 cut(s)	1380
Csp6I	GTAC	2 cut(s)	815, 1602
CviAII	CATG	7 cut(s)	305, 457, 673, 870, 1631, 1903, 1974
CviQI	GTAC	2 cut(s)	815, 1602
DdeI	CTNAG	5 cut(s)	120, 150, 771, 879, 1587
DpnI	GATC	9 cut(s)	30, 189, 362, 533, 609, 677, 724, 1287, 1365
DpnII	GATC	9 cut(s)	28, 187, 360, 531, 607, 675, 722, 1285, 1363
Eam1104I	CTCTTC	3 cut(s)	150, 732, 962
EarI	CTCTTC	3 cut(s)	150, 732, 962
Ecl136II	GAGCTC	2 cut(s)	955, 1883
Eco130I	CCWWGG	3 cut(s)	869, 1622, 1819
Eco147I	AGGCCT	1 cut(s)	790
Eco24I	GRGCYC	3 cut(s)	957, 1835, 1885
Eco31I	GGTCTC	3 cut(s)	80, 101, 206
Eco47I	GGWCC	1 cut(s)	928
Eco53kI	GAGCTC	2 cut(s)	955, 1883
Eco57I	CTGAAG	2 cut(s)	660, 756
Eco88I	CYCGRG	1 cut(s)	932
Eco91I	GGTNACC	2 cut(s)	242, 411
EcoICRI	GAGCTC	2 cut(s)	955, 1883
EcoNI	CCTNNNNNAGG	1 cut(s)	1664
EcoO109I	RGGNCCY	1 cut(s)	928
EcoO65I	GGTNACC	2 cut(s)	242, 411
EcoRI	GAATTC	1 cut(s)	594
EcoRII	CCWGG	5 cut(s)	321, 658, 790, 833, 1324
EcoT14I	CCWWGG	3 cut(s)	869, 1622, 1819
EcoT22I	ATGCAT	1 cut(s)	897
EcoT38I	GRGCYC	3 cut(s)	957, 1835, 1885
ErhI	CCWWGG	3 cut(s)	869, 1622, 1819
FaeI	CATG	7 cut(s)	308, 460, 676, 873, 1634, 1906, 1977
FalI	AAGNNNNNCTT	6 cut(s)	801, 833, 1846, 1878, 1870, 1902
FaqI	GGGAC	3 cut(s)	185, 914, 1891
FatI	CATG	7 cut(s)	304, 456, 672, 869, 1630, 1902, 1973
FauI	CCCGC	1 cut(s)	108
FbaI	TGATCA	4 cut(s)	28, 360, 607, 1363
Fnu4HI	GCNGC	5 cut(s)	104, 177, 842, 1247, 1577
FokI	GGATG	9 cut(s)	321, 454, 725, 1031, 1200, 1212, 1373, 1445, 1802
FriOI	GRGCYC	3 cut(s)	957, 1835, 1885
Fsp4HI	GCNGC	5 cut(s)	104, 177, 842, 1247, 1577
FspBI	CTAG	5 cut(s)	921, 1251, 1292, 1437, 1899
GlaI	GCGC	2 cut(s)	280, 1895
GluI	GCNGC	5 cut(s)	104, 177, 842, 1247, 1577
GsuI	CTGGAG	2 cut(s)	305, 856
HaeII	RGCGCY	1 cut(s)	1897
HaeIII	GGCC	4 cut(s)	50, 790, 867, 1471
HapII	CCGG	3 cut(s)	72, 276, 933
HgaI	GACGC	1 cut(s)	1380
HhaI	GCGC	2 cut(s)	281, 1896
Hin1I	GRCGYC	1 cut(s)	1391
Hin1II	CATG	7 cut(s)	308, 460, 676, 873, 1634, 1906, 1977
Hin6I	GCGC	2 cut(s)	279, 1894
HinP1I	GCGC	2 cut(s)	279, 1894
HincII	GTYRAC	2 cut(s)	1657, 1680
HindII	GTYRAC	2 cut(s)	1657, 1680
HindIII	AAGCTT	3 cut(s)	1492, 1589, 1766
HpaI	GTTAAC	1 cut(s)	1657
HpaII	CCGG	3 cut(s)	72, 276, 933
HphI	GGTGA	8 cut(s)	254, 641, 709, 1094, 1291, 1646, 1747, 2039
Hpy166II	GTNNAC	5 cut(s)	43, 317, 1342, 1657, 1680
Hpy188III	TCNNGA	6 cut(s)	58, 79, 535, 921, 1308, 1943
Hpy8I	GTNNAC	5 cut(s)	43, 317, 1342, 1657, 1680
Hpy99I	CGWCG	3 cut(s)	1063, 1107, 1566
HpyCH4III	ACNGT	3 cut(s)	209, 1518, 1962
HpyF10VI	GCNNNNNNNGC	6 cut(s)	146, 176, 379, 598, 1821, 1830
HpyF3I	CTNAG	5 cut(s)	120, 150, 771, 879, 1587
Hsp92I	GRCGYC	1 cut(s)	1391
Hsp92II	CATG	7 cut(s)	308, 460, 676, 873, 1634, 1906, 1977
HspAI	GCGC	2 cut(s)	279, 1894
KflI	GGGWCCC	1 cut(s)	928
KroI	GCCGGC	1 cut(s)	275
KroNI	GCCGGC	1 cut(s)	277
Ksp22I	TGATCA	4 cut(s)	28, 360, 607, 1363
KspAI	GTTAAC	1 cut(s)	1657
KspI	CCGCGG	1 cut(s)	104
Kzo9I	GATC	9 cut(s)	28, 187, 360, 531, 607, 675, 722, 1285, 1363
LguI	GCTCTTC	1 cut(s)	962
LmnI	GCTCC	3 cut(s)	288, 1573, 1888
Lsp1109I	GCAGC	2 cut(s)	163, 1563
LweI	GCATC	4 cut(s)	157, 1108, 1423, 1833
MaeI	CTAG	5 cut(s)	921, 1251, 1292, 1437, 1899
MaeIII	GTNAC	9 cut(s)	55, 110, 242, 411, 663, 802, 1424, 1753, 2045
MalI	GATC	9 cut(s)	30, 189, 362, 533, 609, 677, 724, 1287, 1365
MboI	GATC	9 cut(s)	28, 187, 360, 531, 607, 675, 722, 1285, 1363
MflI	RGATCY	2 cut(s)	531, 722
MhlI	GDGCHC	6 cut(s)	45, 319, 957, 1122, 1835, 1885
MlyI	GAGTC	3 cut(s)	799, 1708, 2053
MmeI	TCCRAC	1 cut(s)	1041
Mph1103I	ATGCAT	1 cut(s)	897
MroNI	GCCGGC	1 cut(s)	275
MroXI	GAANNNNTTC	1 cut(s)	1938
MseI	TTAA	7 cut(s)	422, 999, 1047, 1466, 1551, 1643, 1656
MslI	CAYNNNNRTG	3 cut(s)	1349, 1431, 1635
MspA1I	CMGCKG	1 cut(s)	103
MspI	CCGG	3 cut(s)	72, 276, 933
MspR9I	CCNGG	7 cut(s)	323, 660, 792, 835, 933, 934, 1326
Mva1269I	GAATGC	1 cut(s)	709
MvaI	CCWGG	5 cut(s)	323, 660, 792, 835, 1326
MvnI	CGCG	2 cut(s)	103, 281
MwoI	GCNNNNNNNGC	6 cut(s)	146, 176, 379, 598, 1821, 1830
NaeI	GCCGGC	1 cut(s)	277
NciI	CCSGG	2 cut(s)	933, 934
NcoI	CCATGG	1 cut(s)	869
NdeII	GATC	9 cut(s)	28, 187, 360, 531, 607, 675, 722, 1285, 1363
NgoMIV	GCCGGC	1 cut(s)	275
NheI	GCTAGC	1 cut(s)	1436
NlaIII	CATG	7 cut(s)	308, 460, 676, 873, 1634, 1906, 1977
NlaIV	GGNNCC	7 cut(s)	654, 724, 929, 930, 1131, 1620, 1734
NmuCI	GTSAC	7 cut(s)	55, 242, 663, 802, 1424, 1753, 2045
NsiI	ATGCAT	1 cut(s)	897
NspI	RCATGY	1 cut(s)	1906
NspV	TTCGAA	1 cut(s)	127
PceI	AGGCCT	1 cut(s)	790
PciSI	GCTCTTC	1 cut(s)	962
PcsI	WCGNNNNNNNCGW	1 cut(s)	1847
PctI	GAATGC	1 cut(s)	709
PdiI	GCCGGC	1 cut(s)	277
PdmI	GAANNNNTTC	1 cut(s)	1938
PfeI	GAWTC	7 cut(s)	9, 85, 124, 134, 1055, 1298, 1420
PflMI	CCANNNNNTGG	1 cut(s)	385
PfoI	TCCNGGA	1 cut(s)	321
PkrI	GCNGC	5 cut(s)	105, 178, 843, 1248, 1578
PleI	GAGTC	3 cut(s)	799, 1707, 2052
PpsI	GAGTC	3 cut(s)	799, 1707, 2052
PpuMI	RGGWCCY	1 cut(s)	928
Psp124BI	GAGCTC	2 cut(s)	957, 1885
Psp5II	RGGWCCY	1 cut(s)	928
Psp6I	CCWGG	5 cut(s)	321, 658, 790, 833, 1324
PspEI	GGTNACC	2 cut(s)	242, 411
PspGI	CCWGG	5 cut(s)	321, 658, 790, 833, 1324
PspN4I	GGNNCC	7 cut(s)	654, 724, 929, 930, 1131, 1620, 1734
PspPI	GGNCC	2 cut(s)	866, 928
PspPPI	RGGWCCY	1 cut(s)	928
PstNI	CAGNNNCTG	1 cut(s)	385
PsuI	RGATCY	2 cut(s)	531, 722
RsaI	GTAC	2 cut(s)	816, 1603
RsaNI	GTAC	2 cut(s)	815, 1602
RseI	CAYNNNNRTG	3 cut(s)	1349, 1431, 1635
SacI	GAGCTC	2 cut(s)	957, 1885
SacII	CCGCGG	1 cut(s)	104
SapI	GCTCTTC	1 cut(s)	962
SaqAI	TTAA	7 cut(s)	422, 999, 1047, 1466, 1551, 1643, 1656
SatI	GCNGC	5 cut(s)	104, 177, 842, 1247, 1577
Sau3AI	GATC	9 cut(s)	28, 187, 360, 531, 607, 675, 722, 1285, 1363
Sau96I	GGNCC	2 cut(s)	866, 928
ScaI	AGTACT	1 cut(s)	816
SchI	GAGTC	3 cut(s)	799, 1708, 2053
ScrFI	CCNGG	7 cut(s)	323, 660, 792, 835, 933, 934, 1326
SduI	GDGCHC	6 cut(s)	45, 319, 957, 1122, 1835, 1885
SfaNI	GCATC	4 cut(s)	157, 1108, 1423, 1833
Sfr303I	CCGCGG	1 cut(s)	104
SfuI	TTCGAA	1 cut(s)	127
SgrBI	CCGCGG	1 cut(s)	104
SinI	GGWCC	1 cut(s)	928
SmaI	CCCGGG	1 cut(s)	934
SmiMI	CAYNNNNRTG	3 cut(s)	1349, 1431, 1635
SmlI	CTYRAG	2 cut(s)	77, 807
SmoI	CTYRAG	2 cut(s)	77, 807
SseBI	AGGCCT	1 cut(s)	790
SsiI	CCGC	7 cut(s)	101, 103, 281, 436, 472, 841, 1247
SspMI	CTAG	5 cut(s)	921, 1251, 1292, 1437, 1899
SstI	GAGCTC	2 cut(s)	957, 1885
StuI	AGGCCT	1 cut(s)	790
StyD4I	CCNGG	7 cut(s)	321, 658, 790, 833, 931, 932, 1324
StyI	CCWWGG	3 cut(s)	869, 1622, 1819
TaaI	ACNGT	3 cut(s)	209, 1518, 1962
TaqI	TCGA	4 cut(s)	12, 127, 503, 1841
TatI	WGTACW	1 cut(s)	814
TauI	GCSGC	3 cut(s)	106, 844, 1249
TfiI	GAWTC	7 cut(s)	9, 85, 124, 134, 1055, 1298, 1420
Tru1I	TTAA	7 cut(s)	422, 999, 1047, 1466, 1551, 1643, 1656
Tru9I	TTAA	7 cut(s)	422, 999, 1047, 1466, 1551, 1643, 1656
TscAI	CASTG	3 cut(s)	390, 1734, 1877
TseFI	GTSAC	7 cut(s)	55, 242, 663, 802, 1424, 1753, 2045
TseI	GCWGC	2 cut(s)	176, 1576
Tsp45I	GTSAC	7 cut(s)	55, 242, 663, 802, 1424, 1753, 2045
TspDTI	ATGAA	9 cut(s)	132, 315, 487, 716, 929, 987, 993, 1400, 1848
TspMI	CCCGGG	1 cut(s)	932
TspRI	CASTG	3 cut(s)	390, 1734, 1877
Van91I	CCANNNNNTGG	1 cut(s)	385
VneI	GTGCAC	2 cut(s)	41, 315
VpaK11BI	GGWCC	1 cut(s)	928
XagI	CCTNNNNNAGG	1 cut(s)	1664
XapI	RAATTY	2 cut(s)	550, 594
XbaI	TCTAGA	1 cut(s)	920
XceI	RCATGY	1 cut(s)	1906
XmaI	CCCGGG	1 cut(s)	932
XmnI	GAANNNNTTC	1 cut(s)	1938
XspI	CTAG	5 cut(s)	921, 1251, 1292, 1437, 1899
ZrmI	AGTACT	1 cut(s)	816
Zsp2I	ATGCAT	1 cut(s)	897

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.