MD13G1285000.v1.1

Ankyrin repeat domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0005480
Physical Location & Seq
Forward (+)
43132363 .. 43133827
1465 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1285000.v1.1.491

Sequence Viewer

Length: 1386 bp
ATGATCATAGTTAGGCACTACCTGCCACTGGCTTGGGCAAAATGGTGCAGAAGGTTACCTCGTTTAATTGGAACAATGCGAAGGATGAGGGATTTCTACATGGAAATTACATTCCACTTTGAGAGTTCTGTTATACCTTTCATTTCTAGGATTGCCCCATCAGATACTTGCAAGATCTGGAAATGGGGTGCAAATTTGAGAGCAGATATGACATTGGCTGGTTTTGATGGTTTGCGAATTCAGCGTTCTGATCAGAGTGTTCTTTTCCTCGGTGATGGGTCTGAGGATGGTATGGTGCCTCCCGGGTCACTTTGCATGATCTCGCACAAGGATAAGGAGGTGATGAATGCTTTGGATGGTGCTGGTTCTCAAGCAACTGAAGAAGAGGTTCGGCAAGAGGTGGTTGCAATGTCTCAGACTAACATATTCAGGCCTGGGATTGATGTGACTCAGACAGTACTTTTACCTCAAATGACCTGGAGGCGGCAGGAGAAAATAGAAATGGTTGGCCCGTGGAAGGCCAGGGTATATGATATGCATAATGTGGTTCTCTTCTCATCCTGTAATGAAAATGAAACAGAAAGTGAGCAGTTTAATGATATTCTGATAGAGGATGAGAGGAGGGAACCTGAAAATGCACTTAAATTGGATTCGTCAGAATTGTCAAACGAAAATGGTGATGGGATTATTGAGCATCGTCATAGTTGTTATGATCACAGGGAGATTCCTATTGAAGATGTTAGCAGTGGTAGAAATGGAGAGAATAAGCAGGAAAAGAAAGGATGGTTTGGTGGATGGAGGAAACGGGGTACTGAAAATGAAGGGCAGAAAAAGATTGTGCCGCCTAGAAGTTCTCTCTGTGTAGATGAGAAGGTGAGTGATCTGCTAGGGGATTCTCTTTCAAGAAATCAAAGCAGACCCGGAAGAAGTAATGTGGACATTATTGTGAAGGGTGATGATCACCGGAGAGGAAGGGATATAAATGCATCTTCTTCCACAAGTTCTGATAACAGGAATCGTCACAAGGGTGCTAGCAAGGAGAATGAGTATAAGAAAGGATTAAGGCCAATACTCTGCCTTTCCCCAAACTTTCCATTACAAACAGAGGAACTGTTGCTGTTACTTGACATTTTAGCAAACAAGGTTAAGGCAATTCGTCGTTTGAGGGAGCTGCTTACAACCAAACTTCCAATGGGTACATTTCCAGTCAAGGTATGCCACCTTCCCATTATCATTTTGTTACCCTATCTGGTTTATGGTTTCCTAGATGCACCTTTATTATCTTTTCCTTTTTATCAAACTTGTTCAAAGAACATTTTGTTATTTTCTTTTTTTACCATGCCTGGTTCTGTTGTAACTGTAAGAGTAGGATGTGCTCTCATCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank_2 Ank_5 GPCR_chapero_1

Protein Analysis

462

Amino Acids

52.45

Weight (kDa)

7.99

Isoelectric Point (pI)

66.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ANKRD13_C PF11904 67 - 411 3e-85 ANKRD13 C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 30
AccB1I GGYRCC 1 cut(s) 295
AciI CCGC 2 cut(s) 484, 842
AcsI RAATTY 2 cut(s) 193, 237
AcuI CTGAAG 1 cut(s) 399
AfaI GTAC 3 cut(s) 459, 811, 1198
AfiI CCNNNNNNNGG 3 cut(s) 28, 483, 517
AgsI TTSAA 3 cut(s) 734, 903, 1308
AjnI CCWGG 4 cut(s) 433, 476, 521, 1342
AleI CACNNNNGTG 1 cut(s) 1026
AluBI AGCT 1 cut(s) 1171
AluI AGCT 1 cut(s) 1171
Alw21I GWGCWC 1 cut(s) 1378
Alw26I GTCTC 1 cut(s) 417
Ama87I CYCGRG 1 cut(s) 302
AoxI GGCC 4 cut(s) 431, 508, 519, 1064
ApeKI GCWGC 1 cut(s) 1171
ApoI RAATTY 2 cut(s) 193, 237
Asp700I GAANNNNTTC 1 cut(s) 387
AspS9I GGNCC 1 cut(s) 509
AsuC2I CCSGG 3 cut(s) 303, 304, 921
AsuHPI GGTGA 6 cut(s) 284, 352, 689, 886, 953, 965
AsuNHI GCTAGC 1 cut(s) 1031
AvaI CYCGRG 1 cut(s) 302
BanI GGYRCC 1 cut(s) 295
BarI GAAGNNNNNNTAC 2 cut(s) 1206, 1238
Bbv12I GWGCWC 1 cut(s) 1378
BbvI GCAGC 1 cut(s) 1158
BccI CCATC 8 cut(s) 166, 221, 269, 281, 350, 674, 777, 789
BciT130I CCWGG 4 cut(s) 435, 478, 523, 1344
BclI TGATCA 4 cut(s) 3, 250, 712, 958
BcnI CCSGG 3 cut(s) 303, 304, 921
BcoDI GTCTC 1 cut(s) 417
BfaI CTAG 5 cut(s) 147, 846, 887, 1032, 1265
BfuAI ACCTGC 1 cut(s) 30
BglII AGATCT 1 cut(s) 174
BisI GCNGC 3 cut(s) 485, 842, 1172
BlsI GCNGC 3 cut(s) 486, 843, 1173
BmcAI AGTACT 1 cut(s) 459
Bme1390I CCNGG 7 cut(s) 303, 304, 435, 478, 523, 921, 1344
BmeT110I CYCGRG 1 cut(s) 302
BmgT120I GGNCC 1 cut(s) 509
BmiI GGNNCC 2 cut(s) 297, 627
BmrFI CCNGG 7 cut(s) 303, 304, 435, 478, 523, 921, 1344
BmsI GCATC 3 cut(s) 703, 995, 1258
BmtI GCTAGC 1 cut(s) 1035
BpmI CTGGAG 1 cut(s) 499
BpuEI CTTGAG 1 cut(s) 354
BpuMI CCSGG 3 cut(s) 303, 304, 921
BsaJI CCNNGG 5 cut(s) 268, 302, 434, 512, 522
BsaWI WCCGGW 1 cut(s) 963
BsaXI ACNNNNNCTCC 2 cut(s) 1031, 1061
Bsc4I CCNNNNNNNGG 3 cut(s) 28, 483, 517
Bse1I ACTGG 2 cut(s) 33, 1205
Bse3DI GCAATG 1 cut(s) 414
BseBI CCWGG 4 cut(s) 435, 478, 523, 1344
BseDI CCNNGG 5 cut(s) 268, 302, 434, 512, 522
BseGI GGATG 8 cut(s) 90, 292, 361, 557, 619, 788, 800, 1376
BseLI CCNNNNNNNGG 3 cut(s) 28, 483, 517
BseMI GCAATG 1 cut(s) 414
BseMII CTCAG 3 cut(s) 273, 428, 464
BseNI ACTGG 2 cut(s) 33, 1205
BseRI GAGGAG 1 cut(s) 634
BseXI GCAGC 1 cut(s) 1158
BsgI GTGCAG 1 cut(s) 67
BshFI GGCC 4 cut(s) 433, 510, 521, 1066
BshNI GGYRCC 1 cut(s) 295
BsiHKAI GWGCWC 1 cut(s) 1378
BsiHKCI CYCGRG 1 cut(s) 302
BsiSI CCGG 3 cut(s) 303, 921, 964
BslI CCNNNNNNNGG 3 cut(s) 28, 483, 517
BsmAI GTCTC 1 cut(s) 417
BsmI GAATGC 1 cut(s) 352
BsnI GGCC 4 cut(s) 433, 510, 521, 1066
BsoBI CYCGRG 1 cut(s) 302
Bsp1286I GDGCHC 1 cut(s) 1378
Bsp143I GATC 7 cut(s) 3, 174, 250, 318, 712, 880, 958
BspACI CCGC 2 cut(s) 484, 842
BspANI GGCC 4 cut(s) 433, 510, 521, 1066
BspCNI CTCAG 3 cut(s) 274, 427, 463
BspLI GGNNCC 2 cut(s) 297, 627
BspMI ACCTGC 1 cut(s) 30
BspOI GCTAGC 1 cut(s) 1035
BspT107I GGYRCC 1 cut(s) 295
BsrDI GCAATG 1 cut(s) 414
BsrI ACTGG 2 cut(s) 33, 1205
BssECI CCNNGG 5 cut(s) 268, 302, 434, 512, 522
BssMI GATC 7 cut(s) 3, 174, 250, 318, 712, 880, 958
Bst2UI CCWGG 4 cut(s) 435, 478, 523, 1344
Bst4CI ACNGT 3 cut(s) 457, 1113, 1360
Bst6I CTCTTC 2 cut(s) 378, 557
BstAPI GCANNNNNTGC 1 cut(s) 22
BstC8I GCNNGC 1 cut(s) 1033
BstDEI CTNAG 3 cut(s) 282, 414, 450
BstDSI CCRYGG 1 cut(s) 512
BstEII GGTNACC 1 cut(s) 54
BstF5I GGATG 8 cut(s) 90, 292, 361, 557, 619, 788, 800, 1376
BstKTI GATC 7 cut(s) 6, 177, 253, 321, 715, 883, 961
BstMAI GTCTC 1 cut(s) 417
BstMBI GATC 7 cut(s) 3, 174, 250, 318, 712, 880, 958
BstMWI GCNNNNNNNGC 2 cut(s) 22, 241
BstNI CCWGG 4 cut(s) 435, 478, 523, 1344
BstPI GGTNACC 1 cut(s) 54
BstSCI CCNGG 7 cut(s) 301, 302, 433, 476, 521, 919, 1342
BstV1I GCAGC 1 cut(s) 1158
BstX2I RGATCY 1 cut(s) 174
BstXI CCANNNNNNTGG 1 cut(s) 33
BstYI RGATCY 1 cut(s) 174
BsuRI GGCC 4 cut(s) 433, 510, 521, 1066
BtgI CCRYGG 1 cut(s) 512
BtsCI GGATG 8 cut(s) 90, 292, 361, 557, 619, 788, 800, 1376
BtsI GCAGTG 1 cut(s) 751
BtsIMutI CAGTG 2 cut(s) 26, 751
BveI ACCTGC 1 cut(s) 30
Cac8I GCNNGC 1 cut(s) 1033
Cfr13I GGNCC 1 cut(s) 509
Cfr9I CCCGGG 1 cut(s) 302
Csp6I GTAC 3 cut(s) 458, 810, 1197
CviAII CATG 3 cut(s) 100, 316, 1339
CviJI RGCY 7 cut(s) 32, 218, 433, 510, 521, 1066, 1171
CviKI_1 RGCY 7 cut(s) 32, 218, 433, 510, 521, 1066, 1171
CviQI GTAC 3 cut(s) 458, 810, 1197
DdeI CTNAG 3 cut(s) 282, 414, 450
DpnI GATC 7 cut(s) 5, 176, 252, 320, 714, 882, 960
DpnII GATC 7 cut(s) 3, 174, 250, 318, 712, 880, 958
Eam1104I CTCTTC 2 cut(s) 378, 557
EarI CTCTTC 2 cut(s) 378, 557
Eco147I AGGCCT 1 cut(s) 433
Eco57I CTGAAG 1 cut(s) 399
Eco88I CYCGRG 1 cut(s) 302
Eco91I GGTNACC 1 cut(s) 54
EcoO65I GGTNACC 1 cut(s) 54
EcoRI GAATTC 1 cut(s) 237
EcoRII CCWGG 4 cut(s) 433, 476, 521, 1342
EcoT22I ATGCAT 2 cut(s) 540, 988
FaeI CATG 3 cut(s) 103, 319, 1342
FatI CATG 3 cut(s) 99, 315, 1338
FbaI TGATCA 4 cut(s) 3, 250, 712, 958
Fnu4HI GCNGC 3 cut(s) 485, 842, 1172
FokI GGATG 7 cut(s) 97, 299, 368, 544, 626, 795, 807
Fsp4HI GCNGC 3 cut(s) 485, 842, 1172
FspBI CTAG 5 cut(s) 147, 846, 887, 1032, 1265
GluI GCNGC 3 cut(s) 485, 842, 1172
GsuI CTGGAG 1 cut(s) 499
HaeIII GGCC 4 cut(s) 433, 510, 521, 1066
HapII CCGG 3 cut(s) 303, 921, 964
Hin1II CATG 3 cut(s) 103, 319, 1342
HinfI GANTC 5 cut(s) 448, 650, 724, 893, 1015
HpaII CCGG 3 cut(s) 303, 921, 964
HphI GGTGA 6 cut(s) 284, 352, 689, 886, 953, 965
Hpy166II GTNNAC 1 cut(s) 937
Hpy188III TCNNGA 2 cut(s) 178, 903
Hpy8I GTNNAC 1 cut(s) 937
Hpy99I CGWCG 1 cut(s) 1161
HpyAV CCTTC 8 cut(s) 45, 75, 511, 815, 865, 943, 966, 1232
HpyCH4III ACNGT 3 cut(s) 457, 1113, 1360
HpyCH4V TGCA 9 cut(s) 48, 171, 191, 315, 407, 538, 638, 986, 1271
HpyF10VI GCNNNNNNNGC 2 cut(s) 22, 241
HpyF3I CTNAG 3 cut(s) 282, 414, 450
Hsp92II CATG 3 cut(s) 103, 319, 1342
Ksp22I TGATCA 4 cut(s) 3, 250, 712, 958
Kzo9I GATC 7 cut(s) 3, 174, 250, 318, 712, 880, 958
LmnI GCTCC 1 cut(s) 1168
Lsp1109I GCAGC 1 cut(s) 1158
LweI GCATC 3 cut(s) 703, 995, 1258
MaeI CTAG 5 cut(s) 147, 846, 887, 1032, 1265
MaeIII GTNAC 7 cut(s) 54, 306, 445, 1019, 1119, 1239, 1354
MalI GATC 7 cut(s) 5, 176, 252, 320, 714, 882, 960
MboI GATC 7 cut(s) 3, 174, 250, 318, 712, 880, 958
MboII GAAGA 7 cut(s) 392, 395, 544, 746, 936, 981, 984
MflI RGATCY 1 cut(s) 174
MhlI GDGCHC 1 cut(s) 1378
MluCI AATT 7 cut(s) 66, 105, 193, 237, 644, 659, 1152
MlyI GAGTC 1 cut(s) 442
Mph1103I ATGCAT 2 cut(s) 540, 988
MroXI GAANNNNTTC 1 cut(s) 387
MseI TTAA 5 cut(s) 65, 594, 642, 1061, 1146
MslI CAYNNNNRTG 2 cut(s) 944, 1026
MspI CCGG 3 cut(s) 303, 921, 964
MspR9I CCNGG 7 cut(s) 303, 304, 435, 478, 523, 921, 1344
Mva1269I GAATGC 1 cut(s) 352
MvaI CCWGG 4 cut(s) 435, 478, 523, 1344
MwoI GCNNNNNNNGC 2 cut(s) 22, 241
NciI CCSGG 3 cut(s) 303, 304, 921
NdeII GATC 7 cut(s) 3, 174, 250, 318, 712, 880, 958
NheI GCTAGC 1 cut(s) 1031
NlaIII CATG 3 cut(s) 103, 319, 1342
NlaIV GGNNCC 2 cut(s) 297, 627
NmuCI GTSAC 3 cut(s) 306, 445, 1019
NsiI ATGCAT 2 cut(s) 540, 988
OliI CACNNNNGTG 1 cut(s) 1026
PceI AGGCCT 1 cut(s) 433
PctI GAATGC 1 cut(s) 352
PdmI GAANNNNTTC 1 cut(s) 387
PfeI GAWTC 4 cut(s) 650, 724, 893, 1015
PkrI GCNGC 3 cut(s) 486, 843, 1173
PleI GAGTC 1 cut(s) 442
PpsI GAGTC 1 cut(s) 442
Psp6I CCWGG 4 cut(s) 433, 476, 521, 1342
PspEI GGTNACC 1 cut(s) 54
PspGI CCWGG 4 cut(s) 433, 476, 521, 1342
PspN4I GGNNCC 2 cut(s) 297, 627
PspPI GGNCC 1 cut(s) 509
PsuI RGATCY 1 cut(s) 174
RsaI GTAC 3 cut(s) 459, 811, 1198
RsaNI GTAC 3 cut(s) 458, 810, 1197
RseI CAYNNNNRTG 2 cut(s) 944, 1026
SaqAI TTAA 5 cut(s) 65, 594, 642, 1061, 1146
SatI GCNGC 3 cut(s) 485, 842, 1172
Sau3AI GATC 7 cut(s) 3, 174, 250, 318, 712, 880, 958
Sau96I GGNCC 1 cut(s) 509
ScaI AGTACT 1 cut(s) 459
SchI GAGTC 1 cut(s) 442
ScrFI CCNGG 7 cut(s) 303, 304, 435, 478, 523, 921, 1344
SduI GDGCHC 1 cut(s) 1378
SfaNI GCATC 3 cut(s) 703, 995, 1258
SmaI CCCGGG 1 cut(s) 304
SmiMI CAYNNNNRTG 2 cut(s) 944, 1026
SmlI CTYRAG 1 cut(s) 369
SmoI CTYRAG 1 cut(s) 369
Sse9I AATT 7 cut(s) 66, 105, 193, 237, 644, 659, 1152
SseBI AGGCCT 1 cut(s) 433
SsiI CCGC 2 cut(s) 484, 842
SspMI CTAG 5 cut(s) 147, 846, 887, 1032, 1265
StuI AGGCCT 1 cut(s) 433
StyD4I CCNGG 7 cut(s) 301, 302, 433, 476, 521, 919, 1342
TaaI ACNGT 3 cut(s) 457, 1113, 1360
TasI AATT 7 cut(s) 66, 105, 193, 237, 644, 659, 1152
TatI WGTACW 1 cut(s) 457
TauI GCSGC 2 cut(s) 487, 844
TfiI GAWTC 4 cut(s) 650, 724, 893, 1015
Tru1I TTAA 5 cut(s) 65, 594, 642, 1061, 1146
Tru9I TTAA 5 cut(s) 65, 594, 642, 1061, 1146
TscAI CASTG 2 cut(s) 33, 751
TseFI GTSAC 3 cut(s) 306, 445, 1019
TseI GCWGC 1 cut(s) 1171
Tsp45I GTSAC 3 cut(s) 306, 445, 1019
TspDTI ATGAA 5 cut(s) 130, 359, 582, 588, 834
TspMI CCCGGG 1 cut(s) 302
TspRI CASTG 2 cut(s) 33, 751
XapI RAATTY 2 cut(s) 193, 237
XcmI CCANNNNNNNNNTGG 1 cut(s) 1189
XmaI CCCGGG 1 cut(s) 302
XmnI GAANNNNTTC 1 cut(s) 387
XspI CTAG 5 cut(s) 147, 846, 887, 1032, 1265
ZrmI AGTACT 1 cut(s) 459
Zsp2I ATGCAT 2 cut(s) 540, 988
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.