MD04G1010500.v1.1

Paired amphipathic helix protein Sin3-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0007501
Physical Location & Seq
Forward (+)
1151590 .. 1153837
2248 bp
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UTR
Exon/CDS
Intron
MD04G1010500.v1.1.491

Sequence Viewer

Length: 342 bp
ATGGAAAACAATCCAAGAGAAAAAGCCTTGGTGTACGTACTCAAACAGTTACAAGGAGTGCAGCATTTCAAGGATGCTCAAGAGAAGTTACAGAAGAGCATATACAACAGAGGGTTTTTCCTCTGCAGAAAAGTTCGGGAAAAACTTGGTAGTTCGGACAAGTTCATTGCGTTCCTAAAGCAACTGCACTTGTATAGCAGCGGAGTTATAGATCTGACCGAGTTTCTGAATCTGGTCGACGATGTACTTCGAGCGGATCAAGATCTTATGAACGAGTTTGATGCTTTCCTGGAGCACTGTGAGAAGGAGCAGATGAATTGGTCGCTTAGGAGTGAAAGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04139 ko04919 ko05016 ko05202 map04139 map04919 map05016 map05202
Pfam Domains
Protein Families
PAH Sin3_corepress Sin3a_C

Protein Analysis

114

Amino Acids

13.35

Weight (kDa)

5.91

Isoelectric Point (pI)

29.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PAH PF02671 54 - 97 2e-07 Paired amphipathic helix repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 254
AccI GTMKAC 1 cut(s) 237
AciI CCGC 2 cut(s) 201, 254
AclWI GGATC 1 cut(s) 264
AfaI GTAC 3 cut(s) 35, 39, 246
AgsI TTSAA 1 cut(s) 70
AjnI CCWGG 1 cut(s) 288
AluBI AGCT 1 cut(s) 339
AluI AGCT 1 cut(s) 339
Alw21I GWGCWC 1 cut(s) 297
AlwI GGATC 1 cut(s) 264
ApeKI GCWGC 2 cut(s) 61, 198
BarI GAAGNNNNNNTAC 2 cut(s) 86, 118
Bbv12I GWGCWC 1 cut(s) 297
BbvI GCAGC 2 cut(s) 73, 210
BcgI CGANNNNNNTGC 2 cut(s) 263, 297
BciT130I CCWGG 1 cut(s) 290
BfmI CTRYAG 1 cut(s) 124
BglII AGATCT 2 cut(s) 211, 262
BisI GCNGC 2 cut(s) 62, 199
BlsI GCNGC 2 cut(s) 63, 200
Bme1390I CCNGG 1 cut(s) 290
BmrFI CCNGG 1 cut(s) 290
BmsI GCATC 2 cut(s) 64, 271
BpmI CTGGAG 1 cut(s) 311
Bpu10I CCTNAGC 1 cut(s) 326
BpuEI CTTGAG 1 cut(s) 63
BsaAI YACGTR 1 cut(s) 37
BsaBI GATNNNNATC 1 cut(s) 261
BsaJI CCNNGG 1 cut(s) 27
Bse3DI GCAATG 1 cut(s) 165
Bse8I GATNNNNATC 1 cut(s) 261
BseBI CCWGG 1 cut(s) 290
BseDI CCNNGG 1 cut(s) 27
BseGI GGATG 1 cut(s) 79
BseJI GATNNNNATC 1 cut(s) 261
BseMI GCAATG 1 cut(s) 165
BseXI GCAGC 2 cut(s) 73, 210
BsgI GTGCAG 2 cut(s) 80, 170
BsiHKAI GWGCWC 1 cut(s) 297
Bsp1286I GDGCHC 1 cut(s) 297
Bsp143I GATC 3 cut(s) 211, 256, 262
BspACI CCGC 2 cut(s) 201, 254
BspMAI CTGCAG 1 cut(s) 128
BspPI GGATC 1 cut(s) 264
BspQI GCTCTTC 1 cut(s) 89
BsrBI CCGCTC 1 cut(s) 254
BsrDI GCAATG 1 cut(s) 165
BssECI CCNNGG 1 cut(s) 27
BssMI GATC 3 cut(s) 211, 256, 262
BssT1I CCWWGG 1 cut(s) 27
Bst2UI CCWGG 1 cut(s) 290
Bst4CI ACNGT 2 cut(s) 48, 299
Bst6I CTCTTC 1 cut(s) 89
BstBAI YACGTR 1 cut(s) 37
BstDEI CTNAG 1 cut(s) 326
BstF5I GGATG 1 cut(s) 79
BstKTI GATC 3 cut(s) 214, 259, 265
BstMBI GATC 3 cut(s) 211, 256, 262
BstNI CCWGG 1 cut(s) 290
BstSCI CCNGG 1 cut(s) 288
BstSFI CTRYAG 1 cut(s) 124
BstSNI TACGTA 1 cut(s) 37
BstV1I GCAGC 2 cut(s) 73, 210
BstX2I RGATCY 2 cut(s) 211, 262
BstYI RGATCY 2 cut(s) 211, 262
BtsCI GGATG 1 cut(s) 79
BtsIMutI CAGTG 1 cut(s) 295
Csp6I GTAC 3 cut(s) 34, 38, 245
CviJI RGCY 2 cut(s) 26, 339
CviKI_1 RGCY 2 cut(s) 26, 339
CviQI GTAC 3 cut(s) 34, 38, 245
DdeI CTNAG 1 cut(s) 326
DpnI GATC 3 cut(s) 213, 258, 264
DpnII GATC 3 cut(s) 211, 256, 262
Eam1104I CTCTTC 1 cut(s) 89
EarI CTCTTC 1 cut(s) 89
Eco105I TACGTA 1 cut(s) 37
Eco130I CCWWGG 1 cut(s) 27
EcoRII CCWGG 1 cut(s) 288
EcoT14I CCWWGG 1 cut(s) 27
ErhI CCWWGG 1 cut(s) 27
FaiI YATR 5 cut(s) 101, 103, 195, 209, 269
FblI GTMKAC 1 cut(s) 237
Fnu4HI GCNGC 2 cut(s) 62, 199
FokI GGATG 1 cut(s) 86
Fsp4HI GCNGC 2 cut(s) 62, 199
GluI GCNGC 2 cut(s) 62, 199
GsuI CTGGAG 1 cut(s) 311
HincII GTYRAC 1 cut(s) 238
HindII GTYRAC 1 cut(s) 238
HinfI GANTC 1 cut(s) 229
Hpy166II GTNNAC 2 cut(s) 34, 238
Hpy188I TCNGA 3 cut(s) 157, 216, 228
Hpy188III TCNNGA 3 cut(s) 80, 137, 260
Hpy8I GTNNAC 2 cut(s) 34, 238
Hpy99I CGWCG 1 cut(s) 242
HpyAV CCTTC 1 cut(s) 298
HpyCH4III ACNGT 2 cut(s) 48, 299
HpyCH4IV ACGT 1 cut(s) 36
HpyCH4V TGCA 3 cut(s) 61, 126, 187
HpyF3I CTNAG 1 cut(s) 326
HpySE526I ACGT 1 cut(s) 36
Kzo9I GATC 3 cut(s) 211, 256, 262
LguI GCTCTTC 1 cut(s) 89
LmnI GCTCC 2 cut(s) 292, 307
LpnPI CCDG 3 cut(s) 218, 275, 302
Lsp1109I GCAGC 2 cut(s) 73, 210
LweI GCATC 2 cut(s) 64, 271
MaeII ACGT 1 cut(s) 36
MaeIII GTNAC 2 cut(s) 48, 87
MalI GATC 3 cut(s) 213, 258, 264
MbiI CCGCTC 1 cut(s) 254
MboI GATC 3 cut(s) 211, 256, 262
MboII GAAGA 1 cut(s) 106
MflI RGATCY 2 cut(s) 211, 262
MhlI GDGCHC 1 cut(s) 297
MluCI AATT 1 cut(s) 316
MnlI CCTC 2 cut(s) 104, 131
MspA1I CMGCKG 1 cut(s) 201
MspR9I CCNGG 1 cut(s) 290
MvaI CCWGG 1 cut(s) 290
NdeII GATC 3 cut(s) 211, 256, 262
PciSI GCTCTTC 1 cut(s) 89
PfeI GAWTC 1 cut(s) 229
PfoI TCCNGGA 1 cut(s) 288
PkrI GCNGC 2 cut(s) 63, 200
Ppu21I YACGTR 1 cut(s) 37
Psp6I CCWGG 1 cut(s) 288
PspGI CCWGG 1 cut(s) 288
PstI CTGCAG 1 cut(s) 128
PsuI RGATCY 2 cut(s) 211, 262
RsaI GTAC 3 cut(s) 35, 39, 246
RsaNI GTAC 3 cut(s) 34, 38, 245
SalI GTCGAC 1 cut(s) 236
SapI GCTCTTC 1 cut(s) 89
SatI GCNGC 2 cut(s) 62, 199
Sau3AI GATC 3 cut(s) 211, 256, 262
ScrFI CCNGG 1 cut(s) 290
SduI GDGCHC 1 cut(s) 297
SetI ASST 2 cut(s) 39, 341
SfaNI GCATC 2 cut(s) 64, 271
SfcI CTRYAG 1 cut(s) 124
SmlI CTYRAG 1 cut(s) 78
SmoI CTYRAG 1 cut(s) 78
SnaBI TACGTA 1 cut(s) 37
Sse9I AATT 1 cut(s) 316
SsiI CCGC 2 cut(s) 201, 254
StyD4I CCNGG 1 cut(s) 288
StyI CCWWGG 1 cut(s) 27
TaaI ACNGT 2 cut(s) 48, 299
TaiI ACGT 1 cut(s) 39
TaqI TCGA 2 cut(s) 237, 250
TaqII GACCGA 1 cut(s) 233
TasI AATT 1 cut(s) 316
TatI WGTACW 1 cut(s) 244
TfiI GAWTC 1 cut(s) 229
TscAI CASTG 1 cut(s) 302
TseI GCWGC 2 cut(s) 61, 198
TspDTI ATGAA 3 cut(s) 154, 284, 329
TspRI CASTG 1 cut(s) 302
XmiI GTMKAC 1 cut(s) 237
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.