Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr04

Orthogroup ID

OG0004808

Physical Location & Seq

Genomic Coordinates Forward (+)

1405403 .. 1409857

Gene Structure 4455 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD04G1012500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 732 bp

ATGGCGTCTCAGTTGCAGAAGATCGAAGGAGACGAATCCGTACTGCTCCGCGTTACACATTCCAATCTGAAGACCTTCTCCGCCGACATCCGCTTCTCCCTCCAGAGCTCCGTCGAGTCCGTCAAGGACAAGCTCTGGCGCAAATGCGGCACCTCCGTCGATTCCATGTCCCTCCACCTCTACGACGACGCCAACACCCTAGTCTCCACTCTCACCGACAACTCCAAACCCCTCGGCTTCTATTCCCCTCACGACGGATTCCGGCTACATGTTGTAGACTTGGATCCTTCGTCGGTGACCTCCGGGGGGTGGCTGGAGGACACATCGTTGGTGGAGAAGTACACCATTTCACAAGAAGCTTATGAGAAGAGGGATGGTACTTTCAGAAAATTTAAGGACAAATTGGTATCGCAGAACCCATCAGCAGTTGAGAACAAAATACCAGAGAACTACATGGAAGATCTCTGTGTGAATATTAAGGTAGGAGATAGATGTGAAGTTGAACCAGGGGCTAAAAGAGGTGTTGTTAAATTTGTCGGTCGGGCGGAACCTCTAGGTCCTGGTTTCTGGATTGGAGTTCAATACGATGAACCCTTGGGAAAGCATGATGGCATGGTGAAGGGAACGCGTTATTTCAGTTGCCCCCCACTTCATGGTGGAATGGTTCGGCCAGAAAAAGTAAAGGTTGGCGACTACCCTGAACGCGATCTATTTGAGGAGGATGAAATTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0007275 GO:0008150 GO:0009524 GO:0009790 GO:0009791 GO:0009793 GO:0009987 GO:0010154 GO:0022414 GO:0032501 GO:0032502 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0048316 GO:0048608 GO:0048731 GO:0048856 GO:0051301 GO:0061458

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

CAP_GLY Ubiquitin_2

Protein Analysis

244

Amino Acids

27.25

Weight (kDa)

5.34

Isoelectric Point (pI)

43.18

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Ubiquitin_2	PF14560	14 - 98	4.3e-29	Ubiquitin-like domain
CAP_GLY	PF01302	161 - 227	1.4e-23	CAP-Gly domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0004808)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G10220
fragaria_vesca	FvH4_4g13730
malus_domestica	MD04G1012500.v1.1
prunus_persica	Prupe.1G158700_v2.0.a1 Prupe.1G158700_v2.0.a1 Prupe.1G158700_v2.0.a1 Prupe.7G257800_v2.0.a1
pyrus_communis	pycom04g01000
rosa_chinensis	RchiOBHm_Chr4g0414381 RchiOBHm_Chr6g0280571
rosa_laevigata	RLG00000008151 RLG00000013048 RLG00000013049 RLG00000013783
rosa_multiflora	Rmu_sc0001432.1_g000007 Rmu_sc0016990.1_g000001 Rmu_sc0029306.1_g000001
rosa_roxburghii	Rroxscaffold_5G00358240 Rroxscaffold_7G00187940
rosa_rugosa	Rorug04G0127600 Rorug04G0127700 Rorug04G0127800
rosa_samantha	Rh4AG185700 Rh4BG185000 Rh4CG198300 Rh6AG245100 Rh6CG249100 Rh6DG239900
rosa_wichuraiana	Rw0G001630 Rw4G015880 Rw6G021210

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	149
AccB7I	CCANNNNNTGG	1 cut(s)	653
AccI	GTMKAC	1 cut(s)	276
AccII	CGCG	3 cut(s)	51, 628, 705
AciI	CCGC	5 cut(s)	49, 81, 91, 147, 545
AclWI	GGATC	2 cut(s)	278, 291
AcoI	YGGCCR	1 cut(s)	668
AcsI	RAATTY	3 cut(s)	389, 530, 726
AcuI	CTGAAG	1 cut(s)	89
AcyI	GRCGYC	2 cut(s)	5, 189
AfaI	GTAC	3 cut(s)	42, 341, 379
AfiI	CCNNNNNNNGG	4 cut(s)	254, 306, 309, 653
AflIII	ACRYGT	2 cut(s)	268, 626
AgsI	TTSAA	2 cut(s)	503, 581
AjnI	CCWGG	2 cut(s)	505, 559
AluBI	AGCT	3 cut(s)	108, 133, 359
AluI	AGCT	3 cut(s)	108, 133, 359
Alw21I	GWGCWC	1 cut(s)	110
Alw26I	GTCTC	3 cut(s)	12, 24, 208
AlwI	GGATC	2 cut(s)	278, 291
AoxI	GGCC	1 cut(s)	668
ApoI	RAATTY	3 cut(s)	389, 530, 726
Asp700I	GAANNNNTTC	1 cut(s)	74
AspLEI	GCGC	1 cut(s)	141
AspS9I	GGNCC	1 cut(s)	557
AsuC2I	CCSGG	1 cut(s)	304
AsuHPI	GGTGA	3 cut(s)	205, 307, 628
AvaII	GGWCC	1 cut(s)	557
BamHI	GGATCC	1 cut(s)	283
BanI	GGYRCC	1 cut(s)	149
BanII	GRGCYC	1 cut(s)	110
BbsI	GAAGAC	1 cut(s)	77
Bbv12I	GWGCWC	1 cut(s)	110
BccI	CCATC	3 cut(s)	368, 427, 602
BcgI	CGANNNNNNTGC	2 cut(s)	139, 173
BciT130I	CCWGG	2 cut(s)	507, 561
BcnI	CCSGG	1 cut(s)	304
BcoDI	GTCTC	3 cut(s)	12, 24, 208
BfaI	CTAG	2 cut(s)	200, 554
BglII	AGATCT	1 cut(s)	460
BisI	GCNGC	1 cut(s)	148
BlsI	GCNGC	1 cut(s)	149
Bme1390I	CCNGG	3 cut(s)	304, 507, 561
Bme18I	GGWCC	1 cut(s)	557
BmgT120I	GGNCC	1 cut(s)	557
BmiI	GGNNCC	3 cut(s)	151, 285, 549
BmrFI	CCNGG	3 cut(s)	304, 507, 561
BpiI	GAAGAC	1 cut(s)	77
BpmI	CTGGAG	2 cut(s)	86, 335
BpuMI	CCSGG	1 cut(s)	304
BsaHI	GRCGYC	2 cut(s)	5, 189
BsaJI	CCNNGG	4 cut(s)	232, 303, 506, 594
Bsc4I	CCNNNNNNNGG	4 cut(s)	254, 306, 309, 653
BseBI	CCWGG	2 cut(s)	507, 561
BseDI	CCNNGG	4 cut(s)	232, 303, 506, 594
BseGI	GGATG	3 cut(s)	87, 379, 727
BseLI	CCNNNNNNNGG	4 cut(s)	254, 306, 309, 653
BseMII	CTCAG	1 cut(s)	23
BseRI	GAGGAG	1 cut(s)	731
Bsh1236I	CGCG	3 cut(s)	51, 628, 705
Bsh1285I	CGRYCG	1 cut(s)	541
BshFI	GGCC	1 cut(s)	670
BshNI	GGYRCC	1 cut(s)	149
BsiEI	CGRYCG	1 cut(s)	541
BsiHKAI	GWGCWC	1 cut(s)	110
BsiSI	CCGG	2 cut(s)	262, 303
BslFI	GGGAC	1 cut(s)	154
BslI	CCNNNNNNNGG	4 cut(s)	254, 306, 309, 653
BsmAI	GTCTC	3 cut(s)	12, 24, 208
BsmBI	CGTCTC	2 cut(s)	12, 24
BsmFI	GGGAC	1 cut(s)	154
BsnI	GGCC	1 cut(s)	670
Bsp1286I	GDGCHC	1 cut(s)	110
Bsp143I	GATC	4 cut(s)	21, 283, 460, 706
BspACI	CCGC	5 cut(s)	49, 81, 91, 147, 545
BspANI	GGCC	1 cut(s)	670
BspCNI	CTCAG	1 cut(s)	22
BspFNI	CGCG	3 cut(s)	51, 628, 705
BspLI	GGNNCC	3 cut(s)	151, 285, 549
BspPI	GGATC	2 cut(s)	278, 291
BspT107I	GGYRCC	1 cut(s)	149
BssECI	CCNNGG	4 cut(s)	232, 303, 506, 594
BssMI	GATC	4 cut(s)	21, 283, 460, 706
BssNI	GRCGYC	2 cut(s)	5, 189
BssT1I	CCWWGG	1 cut(s)	594
Bst2UI	CCWGG	2 cut(s)	507, 561
Bst6I	CTCTTC	1 cut(s)	362
BstACI	GRCGYC	2 cut(s)	5, 189
BstDEI	CTNAG	1 cut(s)	9
BstEII	GGTNACC	1 cut(s)	295
BstF5I	GGATG	3 cut(s)	87, 379, 727
BstFNI	CGCG	3 cut(s)	51, 628, 705
BstHHI	GCGC	1 cut(s)	141
BstKTI	GATC	4 cut(s)	24, 286, 463, 709
BstMAI	GTCTC	3 cut(s)	12, 24, 208
BstMBI	GATC	4 cut(s)	21, 283, 460, 706
BstMCI	CGRYCG	1 cut(s)	541
BstMWI	GCNNNNNNNGC	1 cut(s)	147
BstNI	CCWGG	2 cut(s)	507, 561
BstNSI	RCATGY	1 cut(s)	272
BstPI	GGTNACC	1 cut(s)	295
BstSCI	CCNGG	3 cut(s)	302, 505, 559
BstUI	CGCG	3 cut(s)	51, 628, 705
BstV2I	GAAGAC	1 cut(s)	77
BstX2I	RGATCY	2 cut(s)	283, 460
BstYI	RGATCY	2 cut(s)	283, 460
BsuRI	GGCC	1 cut(s)	670
BtsCI	GGATG	3 cut(s)	87, 379, 727
CfoI	GCGC	1 cut(s)	141
Cfr13I	GGNCC	1 cut(s)	557
CseI	GACGC	1 cut(s)	197
Csp6I	GTAC	3 cut(s)	41, 340, 378
CviAII	CATG	6 cut(s)	166, 269, 454, 605, 613, 653
CviJI	RGCY	8 cut(s)	108, 133, 237, 265, 313, 359, 512, 670
CviKI_1	RGCY	8 cut(s)	108, 133, 237, 265, 313, 359, 512, 670
CviQI	GTAC	3 cut(s)	41, 340, 378
DdeI	CTNAG	1 cut(s)	9
DpnI	GATC	4 cut(s)	23, 285, 462, 708
DpnII	GATC	4 cut(s)	21, 283, 460, 706
EaeI	YGGCCR	1 cut(s)	668
Eam1104I	CTCTTC	1 cut(s)	362
EarI	CTCTTC	1 cut(s)	362
EciI	GGCGGA	2 cut(s)	70, 560
Ecl136II	GAGCTC	1 cut(s)	108
Eco130I	CCWWGG	1 cut(s)	594
Eco24I	GRGCYC	1 cut(s)	110
Eco47I	GGWCC	1 cut(s)	557
Eco53kI	GAGCTC	1 cut(s)	108
Eco57I	CTGAAG	1 cut(s)	89
Eco91I	GGTNACC	1 cut(s)	295
EcoICRI	GAGCTC	1 cut(s)	108
EcoO109I	RGGNCCY	1 cut(s)	557
EcoO65I	GGTNACC	1 cut(s)	295
EcoRII	CCWGG	2 cut(s)	505, 559
EcoT14I	CCWWGG	1 cut(s)	594
EcoT38I	GRGCYC	1 cut(s)	110
ErhI	CCWWGG	1 cut(s)	594
Esp3I	CGTCTC	2 cut(s)	12, 24
FaeI	CATG	6 cut(s)	169, 272, 457, 608, 616, 656
FaiI	YATR	7 cut(s)	167, 270, 363, 455, 606, 614, 654
FaqI	GGGAC	1 cut(s)	154
FatI	CATG	6 cut(s)	165, 268, 453, 604, 612, 652
FblI	GTMKAC	1 cut(s)	276
Fnu4HI	GCNGC	1 cut(s)	148
FokI	GGATG	2 cut(s)	74, 386
FriOI	GRGCYC	1 cut(s)	110
Fsp4HI	GCNGC	1 cut(s)	148
FspBI	CTAG	2 cut(s)	200, 554
GlaI	GCGC	1 cut(s)	140
GluI	GCNGC	1 cut(s)	148
GsuI	CTGGAG	2 cut(s)	86, 335
HaeIII	GGCC	1 cut(s)	670
HapII	CCGG	2 cut(s)	262, 303
HgaI	GACGC	1 cut(s)	197
HhaI	GCGC	1 cut(s)	141
Hin1I	GRCGYC	2 cut(s)	5, 189
Hin1II	CATG	6 cut(s)	169, 272, 457, 608, 616, 656
Hin6I	GCGC	1 cut(s)	139
HinP1I	GCGC	1 cut(s)	139
HindIII	AAGCTT	1 cut(s)	357
HinfI	GANTC	4 cut(s)	35, 116, 161, 258
HpaII	CCGG	2 cut(s)	262, 303
HphI	GGTGA	3 cut(s)	205, 307, 628
Hpy166II	GTNNAC	2 cut(s)	277, 342
Hpy188I	TCNGA	2 cut(s)	69, 386
Hpy188III	TCNNGA	3 cut(s)	103, 251, 568
Hpy8I	GTNNAC	2 cut(s)	277, 342
Hpy99I	CGWCG	6 cut(s)	116, 161, 188, 191, 257, 295
HpyAV	CCTTC	4 cut(s)	20, 85, 297, 613
HpyCH4V	TGCA	1 cut(s)	16
HpyF10VI	GCNNNNNNNGC	1 cut(s)	147
HpyF3I	CTNAG	1 cut(s)	9
Hsp92I	GRCGYC	2 cut(s)	5, 189
Hsp92II	CATG	6 cut(s)	169, 272, 457, 608, 616, 656
HspAI	GCGC	1 cut(s)	139
Kzo9I	GATC	4 cut(s)	21, 283, 460, 706
LmnI	GCTCC	2 cut(s)	51, 113
MaeI	CTAG	2 cut(s)	200, 554
MaeIII	GTNAC	2 cut(s)	52, 295
MalI	GATC	4 cut(s)	23, 285, 462, 708
MboI	GATC	4 cut(s)	21, 283, 460, 706
MboII	GAAGA	4 cut(s)	31, 82, 379, 470
MflI	RGATCY	2 cut(s)	283, 460
MhlI	GDGCHC	1 cut(s)	110
MluCI	AATT	4 cut(s)	389, 401, 530, 726
MluI	ACGCGT	1 cut(s)	626
MlyI	GAGTC	1 cut(s)	125
MroXI	GAANNNNTTC	1 cut(s)	74
MseI	TTAA	4 cut(s)	393, 477, 528, 730
MspI	CCGG	2 cut(s)	262, 303
MspR9I	CCNGG	3 cut(s)	304, 507, 561
MvaI	CCWGG	2 cut(s)	507, 561
MvnI	CGCG	3 cut(s)	51, 628, 705
MwoI	GCNNNNNNNGC	1 cut(s)	147
NciI	CCSGG	1 cut(s)	304
NdeII	GATC	4 cut(s)	21, 283, 460, 706
NlaIII	CATG	6 cut(s)	169, 272, 457, 608, 616, 656
NlaIV	GGNNCC	3 cut(s)	151, 285, 549
NmeAIII	GCCGAG	1 cut(s)	213
NmuCI	GTSAC	1 cut(s)	295
NspI	RCATGY	1 cut(s)	272
PciI	ACATGT	1 cut(s)	268
PcsI	WCGNNNNNNNCGW	1 cut(s)	30
PdmI	GAANNNNTTC	1 cut(s)	74
PfeI	GAWTC	3 cut(s)	35, 161, 258
PflMI	CCANNNNNTGG	1 cut(s)	653
PkrI	GCNGC	1 cut(s)	149
PleI	GAGTC	1 cut(s)	124
PpsI	GAGTC	1 cut(s)	124
PpuMI	RGGWCCY	1 cut(s)	557
PscI	ACATGT	1 cut(s)	268
Psp124BI	GAGCTC	1 cut(s)	110
Psp5II	RGGWCCY	1 cut(s)	557
Psp6I	CCWGG	2 cut(s)	505, 559
PspEI	GGTNACC	1 cut(s)	295
PspGI	CCWGG	2 cut(s)	505, 559
PspN4I	GGNNCC	3 cut(s)	151, 285, 549
PspPI	GGNCC	1 cut(s)	557
PspPPI	RGGWCCY	1 cut(s)	557
PsuI	RGATCY	2 cut(s)	283, 460
RsaI	GTAC	3 cut(s)	42, 341, 379
RsaNI	GTAC	3 cut(s)	41, 340, 378
SacI	GAGCTC	1 cut(s)	110
SaqAI	TTAA	4 cut(s)	393, 477, 528, 730
SatI	GCNGC	1 cut(s)	148
Sau3AI	GATC	4 cut(s)	21, 283, 460, 706
Sau96I	GGNCC	1 cut(s)	557
SchI	GAGTC	1 cut(s)	125
ScrFI	CCNGG	3 cut(s)	304, 507, 561
SduI	GDGCHC	1 cut(s)	110
SinI	GGWCC	1 cut(s)	557
Sse9I	AATT	4 cut(s)	389, 401, 530, 726
SsiI	CCGC	5 cut(s)	49, 81, 91, 147, 545
SspI	AATATT	1 cut(s)	475
SspMI	CTAG	2 cut(s)	200, 554
SstI	GAGCTC	1 cut(s)	110
StyD4I	CCNGG	3 cut(s)	302, 505, 559
StyI	CCWWGG	1 cut(s)	594
TaqI	TCGA	3 cut(s)	24, 114, 159
TaqII	GACCGA	1 cut(s)	527
TasI	AATT	4 cut(s)	389, 401, 530, 726
TatI	WGTACW	1 cut(s)	339
TauI	GCSGC	1 cut(s)	150
TfiI	GAWTC	3 cut(s)	35, 161, 258
Tru1I	TTAA	4 cut(s)	393, 477, 528, 730
Tru9I	TTAA	4 cut(s)	393, 477, 528, 730
TseFI	GTSAC	1 cut(s)	295
Tsp45I	GTSAC	1 cut(s)	295
TspDTI	ATGAA	2 cut(s)	603, 641
TspGWI	ACGGA	5 cut(s)	28, 100, 109, 145, 270
Van91I	CCANNNNNTGG	1 cut(s)	653
VpaK11BI	GGWCC	1 cut(s)	557
XapI	RAATTY	3 cut(s)	389, 530, 726
XceI	RCATGY	1 cut(s)	272
XmiI	GTMKAC	1 cut(s)	276
XmnI	GAANNNNTTC	1 cut(s)	74
XspI	CTAG	2 cut(s)	200, 554

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.