RLG00000013783

Tubulin-folding cofactor B

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0004808
Physical Location & Seq
Forward (+)
41053742 .. 41054818
1077 bp
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UTR
Exon/CDS
Intron
RLM00000013783

Sequence Viewer

Length: 486 bp
ATGGTGTCTCGGCTGCAGAAGATTGAAAAGGACGAATCGGTACTACTGCGAGTGACACATTCCAACCTCAAGACTTTCTCGGCCGAAATCCGCTTCTTCCTTCATAGCACTGTGGAGGCCATCAAAGACAAGCTATGGTGCAAATGCAGCACTTCTGTGAATTTCATGTCTCTTCAACTTTATGACGACGCCAACACCAAACTCGCCGATCTGGCCGACAATTCCAGATCCCTCGGCTTCTATTCCCCTCTCGATGGATTCCGGCTACATATTATCGACTTGGACCCTTCGTCGGTGACCTCCAGTGGGTGGCTGGAGGACACGTCATTGGTGGAGAAGTATACAATTTCCCAAGCTTATGATAAACGCAATGGCACTCTTAGAAAATATAAGGACAAATTGACATTTCAGAATCAATCACATGTTGATAATAAAATACCAGACAACTACATGGAAGATCTCTGTGCGAATATCAAGGTCAGTTAA

Protein Analysis

162

Amino Acids

18.47

Weight (kDa)

6.41

Isoelectric Point (pI)

49.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ubiquitin_2 PF14560 14 - 98 3e-27 Ubiquitin-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 309
AccI GTMKAC 1 cut(s) 341
AciI CCGC 1 cut(s) 91
AclWI GGATC 1 cut(s) 222
AcoI YGGCCR 2 cut(s) 81, 213
AcsI RAATTY 1 cut(s) 160
AcyI GRCGYC 1 cut(s) 189
AfaI GTAC 1 cut(s) 42
AfiI CCNNNNNNNGG 4 cut(s) 254, 292, 306, 309
AflIII ACRYGT 2 cut(s) 321, 421
AgsI TTSAA 2 cut(s) 26, 176
AhdI GACNNNNNGTC 1 cut(s) 289
AjiI CACGTC 1 cut(s) 324
AleI CACNNNNGTG 1 cut(s) 155
AluBI AGCT 2 cut(s) 133, 356
AluI AGCT 2 cut(s) 133, 356
Alw26I GTCTC 2 cut(s) 12, 174
AlwI GGATC 1 cut(s) 222
AoxI GGCC 3 cut(s) 81, 117, 213
ApeKI GCWGC 2 cut(s) 13, 147
ApoI RAATTY 1 cut(s) 160
AspS9I GGNCC 1 cut(s) 283
AsuHPI GGTGA 1 cut(s) 307
AvaII GGWCC 1 cut(s) 283
BbvI GCAGC 1 cut(s) 159
BccI CCATC 2 cut(s) 128, 248
BcoDI GTCTC 2 cut(s) 12, 174
BfmI CTRYAG 1 cut(s) 14
BglI GCCNNNNNGGC 1 cut(s) 212
BglII AGATCT 1 cut(s) 457
BisI GCNGC 2 cut(s) 14, 148
BlsI GCNGC 2 cut(s) 15, 149
Bme18I GGWCC 1 cut(s) 283
BmeRI GACNNNNNGTC 1 cut(s) 289
BmgBI CACGTC 1 cut(s) 324
BmgT120I GGNCC 1 cut(s) 283
BmiI GGNNCC 1 cut(s) 285
BpmI CTGGAG 2 cut(s) 286, 335
BpuEI CTTGAG 1 cut(s) 53
BsaHI GRCGYC 1 cut(s) 189
BsaJI CCNNGG 1 cut(s) 232
BsaXI ACNNNNNCTCC 2 cut(s) 308, 338
Bsc4I CCNNNNNNNGG 4 cut(s) 254, 292, 306, 309
Bse1I ACTGG 1 cut(s) 303
Bse3DI GCAATG 1 cut(s) 376
BseDI CCNNGG 1 cut(s) 232
BseLI CCNNNNNNNGG 4 cut(s) 254, 292, 306, 309
BseMI GCAATG 1 cut(s) 376
BseNI ACTGG 1 cut(s) 303
BseX3I CGGCCG 1 cut(s) 81
BseXI GCAGC 1 cut(s) 159
Bsh1285I CGRYCG 1 cut(s) 84
BshFI GGCC 3 cut(s) 83, 119, 215
BsiEI CGRYCG 1 cut(s) 84
BsiSI CCGG 1 cut(s) 262
BslI CCNNNNNNNGG 4 cut(s) 254, 292, 306, 309
BsmAI GTCTC 2 cut(s) 12, 174
BsnI GGCC 3 cut(s) 83, 119, 215
Bsp143I GATC 3 cut(s) 208, 227, 457
BspACI CCGC 1 cut(s) 91
BspANI GGCC 3 cut(s) 83, 119, 215
BspLI GGNNCC 1 cut(s) 285
BspMAI CTGCAG 1 cut(s) 18
BspPI GGATC 1 cut(s) 222
BsrDI GCAATG 1 cut(s) 376
BsrI ACTGG 1 cut(s) 303
BssECI CCNNGG 1 cut(s) 232
BssMI GATC 3 cut(s) 208, 227, 457
BssNAI GTATAC 1 cut(s) 342
BssNI GRCGYC 1 cut(s) 189
Bst1107I GTATAC 1 cut(s) 342
Bst4CI ACNGT 1 cut(s) 112
Bst6I CTCTTC 1 cut(s) 177
BstACI GRCGYC 1 cut(s) 189
BstDEI CTNAG 1 cut(s) 380
BstEII GGTNACC 1 cut(s) 295
BstKTI GATC 3 cut(s) 211, 230, 460
BstMAI GTCTC 2 cut(s) 12, 174
BstMBI GATC 3 cut(s) 208, 227, 457
BstMCI CGRYCG 1 cut(s) 84
BstMWI GCNNNNNNNGC 2 cut(s) 147, 212
BstNSI RCATGY 1 cut(s) 425
BstPI GGTNACC 1 cut(s) 295
BstSFI CTRYAG 1 cut(s) 14
BstV1I GCAGC 1 cut(s) 159
BstX2I RGATCY 2 cut(s) 227, 457
BstYI RGATCY 2 cut(s) 227, 457
BstZ17I GTATAC 1 cut(s) 342
BstZI CGGCCG 1 cut(s) 81
BsuRI GGCC 3 cut(s) 83, 119, 215
BtrI CACGTC 1 cut(s) 324
BtsIMutI CAGTG 2 cut(s) 108, 310
Cfr13I GGNCC 1 cut(s) 283
CseI GACGC 1 cut(s) 197
Csp6I GTAC 1 cut(s) 41
CviAII CATG 3 cut(s) 166, 422, 451
CviJI RGCY 9 cut(s) 13, 83, 119, 133, 215, 237, 265, 313, 356
CviKI_1 RGCY 9 cut(s) 13, 83, 119, 133, 215, 237, 265, 313, 356
CviQI GTAC 1 cut(s) 41
DdeI CTNAG 1 cut(s) 380
DpnI GATC 3 cut(s) 210, 229, 459
DpnII GATC 3 cut(s) 208, 227, 457
DriI GACNNNNNGTC 1 cut(s) 289
EaeI YGGCCR 2 cut(s) 81, 213
EagI CGGCCG 1 cut(s) 81
Eam1104I CTCTTC 1 cut(s) 177
Eam1105I GACNNNNNGTC 1 cut(s) 289
EarI CTCTTC 1 cut(s) 177
EclXI CGGCCG 1 cut(s) 81
Eco47I GGWCC 1 cut(s) 283
Eco52I CGGCCG 1 cut(s) 81
Eco91I GGTNACC 1 cut(s) 295
EcoO65I GGTNACC 1 cut(s) 295
FaeI CATG 3 cut(s) 169, 425, 454
FatI CATG 3 cut(s) 165, 421, 450
FblI GTMKAC 1 cut(s) 341
Fnu4HI GCNGC 2 cut(s) 14, 148
Fsp4HI GCNGC 2 cut(s) 14, 148
GluI GCNGC 2 cut(s) 14, 148
GsuI CTGGAG 2 cut(s) 286, 335
HaeIII GGCC 3 cut(s) 83, 119, 215
HapII CCGG 1 cut(s) 262
HgaI GACGC 1 cut(s) 197
Hin1I GRCGYC 1 cut(s) 189
Hin1II CATG 3 cut(s) 169, 425, 454
HindIII AAGCTT 1 cut(s) 354
HinfI GANTC 3 cut(s) 35, 258, 412
HpaII CCGG 1 cut(s) 262
HphI GGTGA 1 cut(s) 307
Hpy166II GTNNAC 1 cut(s) 342
Hpy188I TCNGA 1 cut(s) 411
Hpy188III TCNNGA 3 cut(s) 70, 225, 251
Hpy8I GTNNAC 1 cut(s) 342
Hpy99I CGWCG 2 cut(s) 191, 295
HpyAV CCTTC 2 cut(s) 110, 297
HpyCH4III ACNGT 1 cut(s) 112
HpyCH4IV ACGT 1 cut(s) 323
HpyCH4V TGCA 3 cut(s) 16, 141, 147
HpyF10VI GCNNNNNNNGC 2 cut(s) 147, 212
HpyF3I CTNAG 1 cut(s) 380
HpySE526I ACGT 1 cut(s) 323
Hsp92I GRCGYC 1 cut(s) 189
Hsp92II CATG 3 cut(s) 169, 425, 454
Kzo9I GATC 3 cut(s) 208, 227, 457
LpnPI CCDG 6 cut(s) 197, 238, 275, 299, 316, 453
Lsp1109I GCAGC 1 cut(s) 159
MaeII ACGT 1 cut(s) 323
MaeIII GTNAC 2 cut(s) 52, 295
MalI GATC 3 cut(s) 210, 229, 459
MboI GATC 3 cut(s) 208, 227, 457
MboII GAAGA 4 cut(s) 31, 88, 164, 467
MflI RGATCY 2 cut(s) 227, 457
MluCI AATT 4 cut(s) 160, 220, 345, 398
MmeI TCCRAC 1 cut(s) 87
MnlI CCTC 6 cut(s) 77, 109, 242, 258, 310, 310
MseI TTAA 1 cut(s) 484
MslI CAYNNNNRTG 1 cut(s) 155
MspI CCGG 1 cut(s) 262
MwoI GCNNNNNNNGC 2 cut(s) 147, 212
NdeII GATC 3 cut(s) 208, 227, 457
NlaIII CATG 3 cut(s) 169, 425, 454
NlaIV GGNNCC 1 cut(s) 285
NmeAIII GCCGAG 2 cut(s) 59, 213
NmuCI GTSAC 2 cut(s) 52, 295
NspI RCATGY 1 cut(s) 425
OliI CACNNNNGTG 1 cut(s) 155
PciI ACATGT 1 cut(s) 421
PfeI GAWTC 3 cut(s) 35, 258, 412
PflMI CCANNNNNTGG 1 cut(s) 309
PkrI GCNGC 2 cut(s) 15, 149
PscI ACATGT 1 cut(s) 421
PspEI GGTNACC 1 cut(s) 295
PspN4I GGNNCC 1 cut(s) 285
PspPI GGNCC 1 cut(s) 283
PstI CTGCAG 1 cut(s) 18
PsuI RGATCY 2 cut(s) 227, 457
RsaI GTAC 1 cut(s) 42
RsaNI GTAC 1 cut(s) 41
RseI CAYNNNNRTG 1 cut(s) 155
SaqAI TTAA 1 cut(s) 484
SatI GCNGC 2 cut(s) 14, 148
Sau3AI GATC 3 cut(s) 208, 227, 457
Sau96I GGNCC 1 cut(s) 283
SetI ASST 6 cut(s) 69, 135, 302, 326, 358, 480
SfcI CTRYAG 1 cut(s) 14
SinI GGWCC 1 cut(s) 283
SmiMI CAYNNNNRTG 1 cut(s) 155
SmlI CTYRAG 1 cut(s) 68
SmoI CTYRAG 1 cut(s) 68
Sse9I AATT 4 cut(s) 160, 220, 345, 398
SsiI CCGC 1 cut(s) 91
TaaI ACNGT 1 cut(s) 112
TaiI ACGT 1 cut(s) 326
TaqI TCGA 2 cut(s) 252, 276
TasI AATT 4 cut(s) 160, 220, 345, 398
TfiI GAWTC 3 cut(s) 35, 258, 412
Tru1I TTAA 1 cut(s) 484
Tru9I TTAA 1 cut(s) 484
TscAI CASTG 2 cut(s) 115, 310
TseFI GTSAC 2 cut(s) 52, 295
TseI GCWGC 2 cut(s) 13, 147
Tsp45I GTSAC 2 cut(s) 52, 295
TspDTI ATGAA 2 cut(s) 92, 154
TspRI CASTG 2 cut(s) 115, 310
Van91I CCANNNNNTGG 1 cut(s) 309
VpaK11BI GGWCC 1 cut(s) 283
XapI RAATTY 1 cut(s) 160
XceI RCATGY 1 cut(s) 425
XcmI CCANNNNNNNNNTGG 1 cut(s) 310
XmiI GTMKAC 1 cut(s) 341
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.