Rorug04G0127800

Tubulin-folding cofactor B

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0004808
Physical Location & Seq
Forward (+)
20341420 .. 20342160
741 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0127800.1

Sequence Viewer

Length: 741 bp
ATGGAACCACAAACCCAGGAACCAACCGAGGATGGAGGAAGCAACAAAGCAGCCGGGAGTAGTGCTAACATGCTTTGTAGGTGGAACAGGTGGAATCCCACTACAGATCAGATAAGAATCCTCAAGGAGCTTTTCTACAATAAGGGAGTTAGGTCCCCAACTATAGAGCAGATTCAGAGGATCTGTCTCCAGCTGAAATGGTACGGCAAGATCGAGTTCAAGAACGTCTATTTTTGGTTCGTGAACCAAAGGGCTCGGGAGAAGCGGAAGAAGAGGTCCACTTCGGATGTTCATGTGCCCATGCAAAGATCAGGGCTTGTTGGTGATGACAATGGTACCAATTGGAAACCTGAGGATCAGTATATTAACTTCGGATCTTCTGCATCTGCTTCTGCTTCTTCAGCTGGTGTGATTGATTTTAACGGGCAGATAGGGAACTATGGTGGTTATGGATCTATGAACATGGAGAAGAGTGCTAGGGATTGTTCAATCTTAGCTGGAGGGGGAACTAGTTCTACTTTCTTTGACATGAATGTAGAACAAACTTTCATGGAACAAAGAGGAGAAGATCACCAGGAGATTGAAACCCTTTCACTGTTTCCCGTGCATGGTGAGGACATCTTTGACAACATGAAGACTACTTCCGATGAAGGTGGCGGTGACGGTGGTGGTTCTCACATTTCCCTTGAGCTCAGCCTTTCCTGCGAGGGCAAAGCTGGAAAAAATTTCCAGAGCCGCTGA

Protein Analysis

246

Amino Acids

27.36

Weight (kDa)

5.49

Isoelectric Point (pI)

53.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Homeodomain PF00046 27 - 89 2.5e-09 Homeodomain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 335
AccB1I GGYRCC 1 cut(s) 335
AciI CCGC 3 cut(s) 265, 657, 736
AclWI GGATC 4 cut(s) 188, 363, 382, 460
AcsI RAATTY 1 cut(s) 724
AcuI CTGAAG 1 cut(s) 384
AfaI GTAC 2 cut(s) 203, 337
AfiI CCNNNNNNNGG 1 cut(s) 608
AgsI TTSAA 3 cut(s) 220, 489, 584
AhlI ACTAGT 1 cut(s) 509
AjnI CCWGG 2 cut(s) 15, 573
AluBI AGCT 6 cut(s) 130, 193, 404, 497, 691, 716
AluI AGCT 6 cut(s) 130, 193, 404, 497, 691, 716
Alw21I GWGCWC 1 cut(s) 693
Alw26I GTCTC 1 cut(s) 191
AlwI GGATC 4 cut(s) 188, 363, 382, 460
Ama87I CYCGRG 1 cut(s) 255
ApeKI GCWGC 1 cut(s) 50
ApoI RAATTY 1 cut(s) 724
Asp700I GAANNNNTTC 1 cut(s) 511
Asp718I GGTACC 1 cut(s) 335
AspS9I GGNCC 2 cut(s) 153, 276
AsuC2I CCSGG 1 cut(s) 55
AsuHPI GGTGA 4 cut(s) 335, 563, 623, 671
AvaI CYCGRG 1 cut(s) 255
AvaII GGWCC 2 cut(s) 153, 276
AxyI CCTNAGG 1 cut(s) 351
BaeGI GKGCMC 1 cut(s) 300
BaeI ACNNNNGTAYC 2 cut(s) 319, 352
BanI GGYRCC 1 cut(s) 335
BanII GRGCYC 2 cut(s) 256, 693
BarI GAAGNNNNNNTAC 2 cut(s) 353, 385
BbsI GAAGAC 1 cut(s) 641
Bbv12I GWGCWC 1 cut(s) 693
BbvI GCAGC 1 cut(s) 62
BccI CCATC 1 cut(s) 26
BceAI ACGGC 1 cut(s) 220
BciT130I CCWGG 2 cut(s) 17, 575
BcnI CCSGG 1 cut(s) 55
BcoDI GTCTC 1 cut(s) 191
BcuI ACTAGT 1 cut(s) 509
BfaI CTAG 2 cut(s) 477, 510
BfmI CTRYAG 2 cut(s) 102, 162
BisI GCNGC 2 cut(s) 51, 736
BlpI GCTNAGC 1 cut(s) 692
BlsI GCNGC 2 cut(s) 52, 737
Bme1390I CCNGG 3 cut(s) 17, 55, 575
Bme18I GGWCC 2 cut(s) 153, 276
BmeT110I CYCGRG 1 cut(s) 255
BmgT120I GGNCC 2 cut(s) 153, 276
BmiI GGNNCC 4 cut(s) 6, 21, 155, 337
BmrFI CCNGG 3 cut(s) 17, 55, 575
BmsI GCATC 1 cut(s) 392
BpiI GAAGAC 1 cut(s) 641
BpmI CTGGAG 2 cut(s) 173, 519
Bpu1102I GCTNAGC 1 cut(s) 692
BpuEI CTTGAG 2 cut(s) 107, 707
BpuMI CCSGG 1 cut(s) 55
BsaBI GATNNNNATC 1 cut(s) 116
BsaJI CCNNGG 2 cut(s) 15, 27
Bsc4I CCNNNNNNNGG 1 cut(s) 608
Bse21I CCTNAGG 1 cut(s) 351
Bse8I GATNNNNATC 1 cut(s) 116
BseBI CCWGG 2 cut(s) 17, 575
BseDI CCNNGG 2 cut(s) 15, 27
BseGI GGATG 2 cut(s) 37, 292
BseJI GATNNNNATC 1 cut(s) 116
BseLI CCNNNNNNNGG 1 cut(s) 608
BseMII CTCAG 2 cut(s) 342, 706
BseRI GAGGAG 1 cut(s) 576
BseSI GKGCMC 1 cut(s) 300
BseXI GCAGC 1 cut(s) 62
BshNI GGYRCC 1 cut(s) 335
BsiHKAI GWGCWC 1 cut(s) 693
BsiHKCI CYCGRG 1 cut(s) 255
BsiSI CCGG 1 cut(s) 54
BslFI GGGAC 1 cut(s) 139
BslI CCNNNNNNNGG 1 cut(s) 608
BsmAI GTCTC 1 cut(s) 191
BsmFI GGGAC 1 cut(s) 139
BsoBI CYCGRG 1 cut(s) 255
Bsp1286I GDGCHC 3 cut(s) 256, 300, 693
Bsp143I GATC 8 cut(s) 106, 180, 210, 308, 355, 374, 452, 568
Bsp1720I GCTNAGC 1 cut(s) 692
BspACI CCGC 3 cut(s) 265, 657, 736
BspCNI CTCAG 2 cut(s) 343, 705
BspLI GGNNCC 4 cut(s) 6, 21, 155, 337
BspPI GGATC 4 cut(s) 188, 363, 382, 460
BspT107I GGYRCC 1 cut(s) 335
BssECI CCNNGG 2 cut(s) 15, 27
BssMI GATC 8 cut(s) 106, 180, 210, 308, 355, 374, 452, 568
Bst2UI CCWGG 2 cut(s) 17, 575
Bst4CI ACNGT 2 cut(s) 597, 665
Bst6I CTCTTC 2 cut(s) 266, 464
BstDEI CTNAG 3 cut(s) 351, 493, 692
BstF5I GGATG 2 cut(s) 37, 292
BstKTI GATC 8 cut(s) 109, 183, 213, 311, 358, 377, 455, 571
BstMAI GTCTC 1 cut(s) 191
BstMBI GATC 8 cut(s) 106, 180, 210, 308, 355, 374, 452, 568
BstMWI GCNNNNNNNGC 2 cut(s) 401, 702
BstNI CCWGG 2 cut(s) 17, 575
BstNSI RCATGY 1 cut(s) 73
BstSCI CCNGG 3 cut(s) 15, 53, 573
BstSFI CTRYAG 2 cut(s) 102, 162
BstSLI GKGCMC 1 cut(s) 300
BstV1I GCAGC 1 cut(s) 62
BstV2I GAAGAC 1 cut(s) 641
BstX2I RGATCY 3 cut(s) 180, 374, 452
BstYI RGATCY 3 cut(s) 180, 374, 452
Bsu36I CCTNAGG 1 cut(s) 351
BtsCI GGATG 2 cut(s) 37, 292
BtsIMutI CAGTG 1 cut(s) 593
Cfr13I GGNCC 2 cut(s) 153, 276
Csp6I GTAC 2 cut(s) 202, 336
CviAII CATG 8 cut(s) 70, 293, 301, 463, 529, 550, 608, 631
CviQI GTAC 2 cut(s) 202, 336
DdeI CTNAG 3 cut(s) 351, 493, 692
DpnI GATC 8 cut(s) 108, 182, 212, 310, 357, 376, 454, 570
DpnII GATC 8 cut(s) 106, 180, 210, 308, 355, 374, 452, 568
Eam1104I CTCTTC 2 cut(s) 266, 464
EarI CTCTTC 2 cut(s) 266, 464
Ecl136II GAGCTC 1 cut(s) 691
Eco24I GRGCYC 2 cut(s) 256, 693
Eco47I GGWCC 2 cut(s) 153, 276
Eco53kI GAGCTC 1 cut(s) 691
Eco57I CTGAAG 1 cut(s) 384
Eco81I CCTNAGG 1 cut(s) 351
Eco88I CYCGRG 1 cut(s) 255
EcoICRI GAGCTC 1 cut(s) 691
EcoO109I RGGNCCY 1 cut(s) 153
EcoRII CCWGG 2 cut(s) 15, 573
EcoT38I GRGCYC 2 cut(s) 256, 693
FaeI CATG 8 cut(s) 73, 296, 304, 466, 532, 553, 611, 634
FaqI GGGAC 1 cut(s) 139
FatI CATG 8 cut(s) 69, 292, 300, 462, 528, 549, 607, 630
Fnu4HI GCNGC 2 cut(s) 51, 736
FokI GGATG 2 cut(s) 44, 299
FriOI GRGCYC 2 cut(s) 256, 693
Fsp4HI GCNGC 2 cut(s) 51, 736
FspBI CTAG 2 cut(s) 477, 510
GluI GCNGC 2 cut(s) 51, 736
GsuI CTGGAG 2 cut(s) 173, 519
HapII CCGG 1 cut(s) 54
Hin1II CATG 8 cut(s) 73, 296, 304, 466, 532, 553, 611, 634
HinfI GANTC 3 cut(s) 94, 117, 172
HpaII CCGG 1 cut(s) 54
HphI GGTGA 4 cut(s) 335, 563, 623, 671
Hpy166II GTNNAC 2 cut(s) 244, 279
Hpy188I TCNGA 5 cut(s) 111, 177, 286, 374, 646
Hpy188III TCNNGA 4 cut(s) 220, 241, 257, 730
Hpy8I GTNNAC 2 cut(s) 244, 279
HpyAV CCTTC 1 cut(s) 644
HpyCH4III ACNGT 2 cut(s) 597, 665
HpyCH4IV ACGT 1 cut(s) 225
HpyCH4V TGCA 3 cut(s) 304, 383, 607
HpyF10VI GCNNNNNNNGC 2 cut(s) 401, 702
HpyF3I CTNAG 3 cut(s) 351, 493, 692
HpySE526I ACGT 1 cut(s) 225
Hsp92II CATG 8 cut(s) 73, 296, 304, 466, 532, 553, 611, 634
KpnI GGTACC 1 cut(s) 339
Kzo9I GATC 8 cut(s) 106, 180, 210, 308, 355, 374, 452, 568
LmnI GCTCC 1 cut(s) 127
Lsp1109I GCAGC 1 cut(s) 62
LweI GCATC 1 cut(s) 392
MaeI CTAG 2 cut(s) 477, 510
MaeII ACGT 1 cut(s) 225
MaeIII GTNAC 1 cut(s) 659
MalI GATC 8 cut(s) 108, 182, 212, 310, 357, 376, 454, 570
MboI GATC 8 cut(s) 106, 180, 210, 308, 355, 374, 452, 568
MboII GAAGA 7 cut(s) 280, 283, 369, 390, 481, 578, 646
MfeI CAATTG 1 cut(s) 340
MflI RGATCY 3 cut(s) 180, 374, 452
MhlI GDGCHC 3 cut(s) 256, 300, 693
MluCI AATT 2 cut(s) 340, 724
MroXI GAANNNNTTC 1 cut(s) 511
MseI TTAA 2 cut(s) 366, 420
MspA1I CMGCKG 3 cut(s) 193, 404, 738
MspI CCGG 1 cut(s) 54
MspR9I CCNGG 3 cut(s) 17, 55, 575
MunI CAATTG 1 cut(s) 340
MvaI CCWGG 2 cut(s) 17, 575
MwoI GCNNNNNNNGC 2 cut(s) 401, 702
NciI CCSGG 1 cut(s) 55
NdeII GATC 8 cut(s) 106, 180, 210, 308, 355, 374, 452, 568
NlaIII CATG 8 cut(s) 73, 296, 304, 466, 532, 553, 611, 634
NlaIV GGNNCC 4 cut(s) 6, 21, 155, 337
NmuCI GTSAC 1 cut(s) 659
NspI RCATGY 1 cut(s) 73
PcsI WCGNNNNNNNCGW 1 cut(s) 210
PdmI GAANNNNTTC 1 cut(s) 511
PfeI GAWTC 3 cut(s) 94, 117, 172
PkrI GCNGC 2 cut(s) 52, 737
PpuMI RGGWCCY 1 cut(s) 153
Psp124BI GAGCTC 1 cut(s) 693
Psp5II RGGWCCY 1 cut(s) 153
Psp6I CCWGG 2 cut(s) 15, 573
PspGI CCWGG 2 cut(s) 15, 573
PspN4I GGNNCC 4 cut(s) 6, 21, 155, 337
PspPI GGNCC 2 cut(s) 153, 276
PspPPI RGGWCCY 1 cut(s) 153
PsrI GAACNNNNNNTAC 2 cut(s) 499, 531
PsuI RGATCY 3 cut(s) 180, 374, 452
PvuII CAGCTG 2 cut(s) 193, 404
RsaI GTAC 2 cut(s) 203, 337
RsaNI GTAC 2 cut(s) 202, 336
SacI GAGCTC 1 cut(s) 693
SaqAI TTAA 2 cut(s) 366, 420
SatI GCNGC 2 cut(s) 51, 736
Sau3AI GATC 8 cut(s) 106, 180, 210, 308, 355, 374, 452, 568
Sau96I GGNCC 2 cut(s) 153, 276
ScrFI CCNGG 3 cut(s) 17, 55, 575
SduI GDGCHC 3 cut(s) 256, 300, 693
SfaNI GCATC 1 cut(s) 392
SfcI CTRYAG 2 cut(s) 102, 162
SinI GGWCC 2 cut(s) 153, 276
SmlI CTYRAG 2 cut(s) 122, 686
SmoI CTYRAG 2 cut(s) 122, 686
SpeI ACTAGT 1 cut(s) 509
Sse9I AATT 2 cut(s) 340, 724
SsiI CCGC 3 cut(s) 265, 657, 736
SspMI CTAG 2 cut(s) 477, 510
SstI GAGCTC 1 cut(s) 693
StyD4I CCNGG 3 cut(s) 15, 53, 573
TaaI ACNGT 2 cut(s) 597, 665
TaiI ACGT 1 cut(s) 228
TaqI TCGA 1 cut(s) 213
TasI AATT 2 cut(s) 340, 724
TauI GCSGC 1 cut(s) 738
TfiI GAWTC 3 cut(s) 94, 117, 172
Tru1I TTAA 2 cut(s) 366, 420
Tru9I TTAA 2 cut(s) 366, 420
TscAI CASTG 1 cut(s) 600
TseFI GTSAC 1 cut(s) 659
TseI GCWGC 1 cut(s) 50
Tsp45I GTSAC 1 cut(s) 659
TspDTI ATGAA 6 cut(s) 281, 473, 538, 545, 647, 663
TspRI CASTG 1 cut(s) 600
VpaK11BI GGWCC 2 cut(s) 153, 276
XapI RAATTY 1 cut(s) 724
XceI RCATGY 1 cut(s) 73
XmnI GAANNNNTTC 1 cut(s) 511
XspI CTAG 2 cut(s) 477, 510
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.