RLG00000013049

Tubulin-folding cofactor B

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0004808
Physical Location & Seq
Reverse (-)
25356155 .. 25357598
1444 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000013049

Sequence Viewer

Length: 543 bp
ATGGCGTCTCGGCTGCAGAAGATCGAAACGGACGAATCGGTACTACTGCGAGTGACGCATTCCAACCTCAAGACCTTCTCCGCTGAAATCCGGTTCTCCCTGCAGAGCACCGTGGAGGCCGTGAAAGACAAGCTATGGCGCAAATGCGGCACTTCTGTAAATTCCATGTCTCTTCATCTCTACGACGATGCCAACACCAAAGTCGCCGATCTCGCCGACAATTCCAGACCCCTCGGCTTCTATTCCCCTCTCGACGGGTTCCGGCTACATATTATAGACCTGGACCCATCGTCGGTGACCTCCGGCGGGTGGCTGGAGGACACGTCGTTGGTGGAGAAGTACACGATTTCCCAGGAAGCTTATGAGAAACGCGATGGCACTTTTAAAAAGTTTAAGGACAAATTGGCATCTCAGTATACATCAGCTGTTGATAATAAAATACCAGACAACTACATGGAAGACCTCTGTGTAAATATTAAGGTAGGAGATAGATGTCAAGTTGAACCAGGGGACAAAAGAGGTGTTGTTAAATTTGTCGGCTGA

Protein Analysis

181

Amino Acids

20.24

Weight (kDa)

5.95

Isoelectric Point (pI)

41.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ubiquitin_2 PF14560 14 - 98 5.4e-30 Ubiquitin-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 416
AccII CGCG 1 cut(s) 372
AciI CCGC 3 cut(s) 81, 147, 306
AcsI RAATTY 2 cut(s) 160, 530
AcyI GRCGYC 1 cut(s) 5
AfaI GTAC 2 cut(s) 42, 341
AfiI CCNNNNNNNGG 4 cut(s) 254, 292, 306, 309
AflIII ACRYGT 1 cut(s) 321
AgsI TTSAA 1 cut(s) 503
AhdI GACNNNNNGTC 1 cut(s) 289
AjiI CACGTC 1 cut(s) 324
AjnI CCWGG 3 cut(s) 279, 351, 505
AluBI AGCT 3 cut(s) 133, 359, 425
AluI AGCT 3 cut(s) 133, 359, 425
Alw21I GWGCWC 1 cut(s) 110
Alw26I GTCTC 2 cut(s) 12, 174
AoxI GGCC 1 cut(s) 117
ApeKI GCWGC 1 cut(s) 13
ApoI RAATTY 2 cut(s) 160, 530
AspLEI GCGC 1 cut(s) 141
AspS9I GGNCC 1 cut(s) 283
AsuHPI GGTGA 1 cut(s) 307
AvaII GGWCC 1 cut(s) 283
BbsI GAAGAC 1 cut(s) 465
Bbv12I GWGCWC 1 cut(s) 110
BccI CCATC 2 cut(s) 295, 368
BceAI ACGGC 1 cut(s) 104
BciT130I CCWGG 3 cut(s) 281, 353, 507
BcoDI GTCTC 2 cut(s) 12, 174
BfmI CTRYAG 2 cut(s) 14, 101
BisI GCNGC 2 cut(s) 14, 148
BlsI GCNGC 2 cut(s) 15, 149
Bme1390I CCNGG 3 cut(s) 281, 353, 507
Bme18I GGWCC 1 cut(s) 283
BmeRI GACNNNNNGTC 1 cut(s) 289
BmgBI CACGTC 1 cut(s) 324
BmgT120I GGNCC 1 cut(s) 283
BmiI GGNNCC 2 cut(s) 260, 285
BmrFI CCNGG 3 cut(s) 281, 353, 507
BmsI GCATC 2 cut(s) 178, 416
BpiI GAAGAC 1 cut(s) 465
BpmI CTGGAG 1 cut(s) 335
BpuEI CTTGAG 1 cut(s) 53
BsaHI GRCGYC 1 cut(s) 5
BsaJI CCNNGG 4 cut(s) 111, 232, 351, 506
BsaWI WCCGGW 1 cut(s) 90
BsaXI ACNNNNNCTCC 2 cut(s) 308, 338
Bsc4I CCNNNNNNNGG 4 cut(s) 254, 292, 306, 309
BseBI CCWGG 3 cut(s) 281, 353, 507
BseDI CCNNGG 4 cut(s) 111, 232, 351, 506
BseLI CCNNNNNNNGG 4 cut(s) 254, 292, 306, 309
BseMII CTCAG 1 cut(s) 425
Bsh1236I CGCG 1 cut(s) 372
BshFI GGCC 1 cut(s) 119
BsiHKAI GWGCWC 1 cut(s) 110
BsiSI CCGG 3 cut(s) 91, 262, 303
BslFI GGGAC 1 cut(s) 524
BslI CCNNNNNNNGG 4 cut(s) 254, 292, 306, 309
BsmAI GTCTC 2 cut(s) 12, 174
BsmBI CGTCTC 1 cut(s) 12
BsmFI GGGAC 1 cut(s) 524
BsmI GAATGC 1 cut(s) 58
BsnI GGCC 1 cut(s) 119
Bsp1286I GDGCHC 1 cut(s) 110
Bsp143I GATC 2 cut(s) 21, 208
BspACI CCGC 3 cut(s) 81, 147, 306
BspANI GGCC 1 cut(s) 119
BspCNI CTCAG 1 cut(s) 424
BspFNI CGCG 1 cut(s) 372
BspLI GGNNCC 2 cut(s) 260, 285
BspMAI CTGCAG 2 cut(s) 18, 105
BssECI CCNNGG 4 cut(s) 111, 232, 351, 506
BssMI GATC 2 cut(s) 21, 208
BssNAI GTATAC 1 cut(s) 417
BssNI GRCGYC 1 cut(s) 5
Bst1107I GTATAC 1 cut(s) 417
Bst2UI CCWGG 3 cut(s) 281, 353, 507
Bst4CI ACNGT 1 cut(s) 112
Bst6I CTCTTC 1 cut(s) 177
BstACI GRCGYC 1 cut(s) 5
BstDEI CTNAG 1 cut(s) 411
BstDSI CCRYGG 1 cut(s) 111
BstEII GGTNACC 1 cut(s) 295
BstFNI CGCG 1 cut(s) 372
BstHHI GCGC 1 cut(s) 141
BstKTI GATC 2 cut(s) 24, 211
BstMAI GTCTC 2 cut(s) 12, 174
BstMBI GATC 2 cut(s) 21, 208
BstMWI GCNNNNNNNGC 3 cut(s) 55, 147, 212
BstNI CCWGG 3 cut(s) 281, 353, 507
BstPI GGTNACC 1 cut(s) 295
BstSCI CCNGG 3 cut(s) 279, 351, 505
BstSFI CTRYAG 2 cut(s) 14, 101
BstUI CGCG 1 cut(s) 372
BstV2I GAAGAC 1 cut(s) 465
BstZ17I GTATAC 1 cut(s) 417
BsuRI GGCC 1 cut(s) 119
BtgI CCRYGG 1 cut(s) 111
BtgZI GCGATG 1 cut(s) 387
BtrI CACGTC 1 cut(s) 324
CfoI GCGC 1 cut(s) 141
Cfr13I GGNCC 1 cut(s) 283
CseI GACGC 1 cut(s) 64
Csp6I GTAC 2 cut(s) 41, 340
CviAII CATG 2 cut(s) 166, 454
CviJI RGCY 9 cut(s) 13, 119, 133, 237, 265, 313, 359, 425, 540
CviKI_1 RGCY 9 cut(s) 13, 119, 133, 237, 265, 313, 359, 425, 540
CviQI GTAC 2 cut(s) 41, 340
DdeI CTNAG 1 cut(s) 411
DpnI GATC 2 cut(s) 23, 210
DpnII GATC 2 cut(s) 21, 208
DraI TTTAAA 1 cut(s) 385
DriI GACNNNNNGTC 1 cut(s) 289
Eam1104I CTCTTC 1 cut(s) 177
Eam1105I GACNNNNNGTC 1 cut(s) 289
EarI CTCTTC 1 cut(s) 177
Eco47I GGWCC 1 cut(s) 283
Eco91I GGTNACC 1 cut(s) 295
EcoO65I GGTNACC 1 cut(s) 295
EcoRII CCWGG 3 cut(s) 279, 351, 505
Esp3I CGTCTC 1 cut(s) 12
FaeI CATG 2 cut(s) 169, 457
FaiI YATR 7 cut(s) 136, 167, 270, 275, 363, 417, 455
FaqI GGGAC 1 cut(s) 524
FatI CATG 2 cut(s) 165, 453
FauI CCCGC 1 cut(s) 299
FblI GTMKAC 1 cut(s) 416
Fnu4HI GCNGC 2 cut(s) 14, 148
Fsp4HI GCNGC 2 cut(s) 14, 148
GlaI GCGC 1 cut(s) 140
GluI GCNGC 2 cut(s) 14, 148
GsuI CTGGAG 1 cut(s) 335
HaeIII GGCC 1 cut(s) 119
HapII CCGG 3 cut(s) 91, 262, 303
HgaI GACGC 1 cut(s) 64
HhaI GCGC 1 cut(s) 141
Hin1I GRCGYC 1 cut(s) 5
Hin1II CATG 2 cut(s) 169, 457
Hin6I GCGC 1 cut(s) 139
HinP1I GCGC 1 cut(s) 139
HindIII AAGCTT 1 cut(s) 357
HinfI GANTC 1 cut(s) 35
HpaII CCGG 3 cut(s) 91, 262, 303
HphI GGTGA 1 cut(s) 307
Hpy166II GTNNAC 2 cut(s) 342, 417
Hpy188III TCNNGA 3 cut(s) 70, 225, 251
Hpy8I GTNNAC 2 cut(s) 342, 417
Hpy99I CGWCG 4 cut(s) 188, 257, 295, 328
HpyAV CCTTC 1 cut(s) 85
HpyCH4III ACNGT 1 cut(s) 112
HpyCH4IV ACGT 1 cut(s) 323
HpyCH4V TGCA 2 cut(s) 16, 103
HpyF10VI GCNNNNNNNGC 3 cut(s) 55, 147, 212
HpyF3I CTNAG 1 cut(s) 411
HpySE526I ACGT 1 cut(s) 323
Hsp92I GRCGYC 1 cut(s) 5
Hsp92II CATG 2 cut(s) 169, 457
HspAI GCGC 1 cut(s) 139
Kzo9I GATC 2 cut(s) 21, 208
LweI GCATC 2 cut(s) 178, 416
MaeII ACGT 1 cut(s) 323
MaeIII GTNAC 2 cut(s) 52, 295
MalI GATC 2 cut(s) 23, 210
MboI GATC 2 cut(s) 21, 208
MboII GAAGA 3 cut(s) 31, 164, 470
MhlI GDGCHC 1 cut(s) 110
MluCI AATT 4 cut(s) 160, 220, 401, 530
MmeI TCCRAC 1 cut(s) 87
MnlI CCTC 8 cut(s) 77, 109, 242, 258, 310, 310, 473, 512
MseI TTAA 4 cut(s) 384, 393, 477, 528
MspA1I CMGCKG 2 cut(s) 83, 425
MspI CCGG 3 cut(s) 91, 262, 303
MspR9I CCNGG 3 cut(s) 281, 353, 507
Mva1269I GAATGC 1 cut(s) 58
MvaI CCWGG 3 cut(s) 281, 353, 507
MvnI CGCG 1 cut(s) 372
MwoI GCNNNNNNNGC 3 cut(s) 55, 147, 212
NdeII GATC 2 cut(s) 21, 208
NlaIII CATG 2 cut(s) 169, 457
NlaIV GGNNCC 2 cut(s) 260, 285
NmeAIII GCCGAG 1 cut(s) 213
NmuCI GTSAC 2 cut(s) 52, 295
PcsI WCGNNNNNNNCGW 1 cut(s) 30
PctI GAATGC 1 cut(s) 58
PfeI GAWTC 1 cut(s) 35
PkrI GCNGC 2 cut(s) 15, 149
Psp6I CCWGG 3 cut(s) 279, 351, 505
PspEI GGTNACC 1 cut(s) 295
PspGI CCWGG 3 cut(s) 279, 351, 505
PspN4I GGNNCC 2 cut(s) 260, 285
PspPI GGNCC 1 cut(s) 283
PstI CTGCAG 2 cut(s) 18, 105
PvuII CAGCTG 1 cut(s) 425
RsaI GTAC 2 cut(s) 42, 341
RsaNI GTAC 2 cut(s) 41, 340
SaqAI TTAA 4 cut(s) 384, 393, 477, 528
SatI GCNGC 2 cut(s) 14, 148
Sau3AI GATC 2 cut(s) 21, 208
Sau96I GGNCC 1 cut(s) 283
ScrFI CCNGG 3 cut(s) 281, 353, 507
SduI GDGCHC 1 cut(s) 110
SfaNI GCATC 2 cut(s) 178, 416
SfcI CTRYAG 2 cut(s) 14, 101
SinI GGWCC 1 cut(s) 283
SmlI CTYRAG 1 cut(s) 68
SmoI CTYRAG 1 cut(s) 68
Sse9I AATT 4 cut(s) 160, 220, 401, 530
SsiI CCGC 3 cut(s) 81, 147, 306
SspI AATATT 1 cut(s) 475
StyD4I CCNGG 3 cut(s) 279, 351, 505
TaaI ACNGT 1 cut(s) 112
TaiI ACGT 1 cut(s) 326
TaqI TCGA 2 cut(s) 24, 252
TasI AATT 4 cut(s) 160, 220, 401, 530
TatI WGTACW 1 cut(s) 339
TauI GCSGC 1 cut(s) 150
TfiI GAWTC 1 cut(s) 35
Tru1I TTAA 4 cut(s) 384, 393, 477, 528
Tru9I TTAA 4 cut(s) 384, 393, 477, 528
TseFI GTSAC 2 cut(s) 52, 295
TseI GCWGC 1 cut(s) 13
Tsp45I GTSAC 2 cut(s) 52, 295
TspDTI ATGAA 1 cut(s) 164
TspGWI ACGGA 1 cut(s) 44
VpaK11BI GGWCC 1 cut(s) 283
XapI RAATTY 2 cut(s) 160, 530
XmiI GTMKAC 1 cut(s) 416
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.