MD04G1016600.v1.1

Chaperone protein DNAj

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0012219
Physical Location & Seq
Forward (+)
1800728 .. 1802629
1902 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1016600.v1.1.491

Sequence Viewer

Length: 636 bp
ATGCAGAGCTATGGATTAGCAATCTCAGGAACGGGAAGCGATTCCTGCTTCCACATCCCAACATCCCACACAACCATCCCTACCAACATACCCATACCCGACCCCAAACCCAGCTTCCTCTTCCCCAAATCGGTTTCCTCCTTCCGGAACCTGTCCGGGCCCCTCAGGCTCAAAGCGAAGGCCGCCATCAACGATGGGTGGGTGGCGGAGTACAAGGAGCTCACCTTCTACGAGCTTCTGGGCATACCCGAATCCGGATCCGTGACCGAAATCAAGCAGGCGTATAAGCAGCTGGCCCGGAAGTACCATCCGGACGTGTCGCCTCCGGGTCTGGTGGAGGAGTATACGGAGAAGTTTATCCGGGTTCAGGAGGCTTACGAGACCTTGTCTGATTCGAGAAGGAGAGCTTTATATGATCAAGACATGGCCAGGGGTCTTCACCTTGCCTTCTCTGCAAGACGATGCCAATACGATGAGGGAGAGGAGGATGTCGGCAGCTGGAGGAATCGATGGCAAAGTCAGCTTTCTGAGTTGAAGAGAAGAAGCATGAACAAGGATGCTGCTACAAGAGAAGCACATATGTCATGGGGAGCTCGAATGCGCCGGCAAAGAGAAGGATCGTCCGATCAACTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DnaJ

Protein Analysis

212

Amino Acids

24.06

Weight (kDa)

8.81

Isoelectric Point (pI)

64.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DnaJ PF00226 76 - 139 2.7e-20 DnaJ domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012219)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G13830 AT4G13830 AT4G13830
fragaria_vesca FvH4_4g13370
malus_domestica MD04G1016600.v1.1
prunus_persica Prupe.1G163300_v2.0.a1 Prupe.1G163300_v2.0.a1 Prupe.1G204200_v2.0.a1
pyrus_communis pycom04g01270
rosa_chinensis RchiOBHm_Chr4g0413741
rosa_laevigata RLG00000008197
rosa_multiflora Rmu_co8100182.1_g000001
rosa_roxburghii Rroxscaffold_5G00357660
rosa_rugosa Rorug04G0122800
rosa_samantha Rh4AG182200 Rh4BG181300 Rh4CG193400 Rh4DG177800
rosa_wichuraiana Rw4G015250

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 344
AccIII TCCGGA 3 cut(s) 144, 254, 310
AciI CCGC 2 cut(s) 183, 206
AclWI GGATC 3 cut(s) 252, 265, 625
AcoI YGGCCR 1 cut(s) 426
AfaI GTAC 2 cut(s) 212, 305
AfiI CCNNNNNNNGG 4 cut(s) 130, 144, 254, 367
AflIII ACRYGT 1 cut(s) 315
AgsI TTSAA 1 cut(s) 535
AjiI CACGTC 1 cut(s) 316
AjnI CCWGG 1 cut(s) 428
AjuI GAANNNNNNNTTGG 2 cut(s) 98, 130
AluBI AGCT 9 cut(s) 9, 114, 220, 235, 292, 407, 498, 523, 593
AluI AGCT 9 cut(s) 9, 114, 220, 235, 292, 407, 498, 523, 593
Alw21I GWGCWC 2 cut(s) 222, 595
Alw26I GTCTC 1 cut(s) 374
AlwI GGATC 3 cut(s) 252, 265, 625
Aor13HI TCCGGA 3 cut(s) 144, 254, 310
AoxI GGCC 4 cut(s) 158, 180, 294, 426
ApaI GGGCCC 1 cut(s) 162
ApeKI GCWGC 3 cut(s) 289, 495, 560
Asp700I GAANNNNTTC 1 cut(s) 40
AspLEI GCGC 1 cut(s) 603
AspS9I GGNCC 3 cut(s) 158, 159, 295
AsuC2I CCSGG 4 cut(s) 157, 298, 327, 362
AsuHPI GGTGA 2 cut(s) 214, 431
AxyI CCTNAGG 1 cut(s) 164
BaeGI GKGCMC 1 cut(s) 162
BalI TGGCCA 1 cut(s) 428
BamHI GGATCC 1 cut(s) 257
BanII GRGCYC 3 cut(s) 162, 222, 595
BbsI GAAGAC 1 cut(s) 428
Bbv12I GWGCWC 2 cut(s) 222, 595
BbvI GCAGC 3 cut(s) 301, 507, 547
BccI CCATC 5 cut(s) 83, 188, 194, 315, 504
BciT130I CCWGG 1 cut(s) 430
BclI TGATCA 1 cut(s) 415
BcnI CCSGG 4 cut(s) 157, 298, 327, 362
BcoDI GTCTC 1 cut(s) 374
BglI GCCNNNNNGGC 1 cut(s) 166
BisI GCNGC 4 cut(s) 183, 290, 496, 561
BlsI GCNGC 4 cut(s) 184, 291, 497, 562
Bme1390I CCNGG 5 cut(s) 157, 298, 327, 362, 430
BmgBI CACGTC 1 cut(s) 316
BmgT120I GGNCC 3 cut(s) 158, 159, 295
BmiI GGNNCC 4 cut(s) 149, 160, 161, 259
BmrFI CCNGG 5 cut(s) 157, 298, 327, 362, 430
BmsI GCATC 2 cut(s) 452, 547
BpiI GAAGAC 1 cut(s) 428
BpmI CTGGAG 1 cut(s) 520
BpuMI CCSGG 4 cut(s) 157, 298, 327, 362
Bsa29I ATCGAT 1 cut(s) 508
BsaI GGTCTC 1 cut(s) 374
BsaJI CCNNGG 1 cut(s) 429
BsaWI WCCGGW 3 cut(s) 144, 254, 310
Bsc4I CCNNNNNNNGG 4 cut(s) 130, 144, 254, 367
Bse118I RCCGGY 1 cut(s) 603
Bse21I CCTNAGG 1 cut(s) 164
BseAI TCCGGA 3 cut(s) 144, 254, 310
BseBI CCWGG 1 cut(s) 430
BseCI ATCGAT 1 cut(s) 508
BseDI CCNNGG 1 cut(s) 429
BseGI GGATG 6 cut(s) 54, 62, 75, 307, 493, 562
BseLI CCNNNNNNNGG 4 cut(s) 130, 144, 254, 367
BseMII CTCAG 3 cut(s) 39, 178, 519
BseRI GAGGAG 2 cut(s) 353, 497
BseSI GKGCMC 1 cut(s) 162
BseXI GCAGC 3 cut(s) 301, 507, 547
BseYI CCCAGC 1 cut(s) 110
BshFI GGCC 4 cut(s) 160, 182, 296, 428
BshVI ATCGAT 1 cut(s) 508
BsiHKAI GWGCWC 2 cut(s) 222, 595
BsiSI CCGG 8 cut(s) 145, 156, 255, 298, 311, 326, 361, 604
BslI CCNNNNNNNGG 4 cut(s) 130, 144, 254, 367
BsmAI GTCTC 1 cut(s) 374
BsmI GAATGC 1 cut(s) 603
BsnI GGCC 4 cut(s) 160, 182, 296, 428
Bso31I GGTCTC 1 cut(s) 374
Bsp120I GGGCCC 1 cut(s) 158
Bsp1286I GDGCHC 3 cut(s) 162, 222, 595
Bsp13I TCCGGA 3 cut(s) 144, 254, 310
Bsp143I GATC 4 cut(s) 257, 415, 617, 625
BspACI CCGC 2 cut(s) 183, 206
BspANI GGCC 4 cut(s) 160, 182, 296, 428
BspCNI CTCAG 3 cut(s) 38, 177, 520
BspDI ATCGAT 1 cut(s) 508
BspEI TCCGGA 3 cut(s) 144, 254, 310
BspLI GGNNCC 4 cut(s) 149, 160, 161, 259
BspPI GGATC 3 cut(s) 252, 265, 625
BspTNI GGTCTC 1 cut(s) 374
BsrFI RCCGGY 1 cut(s) 603
BssAI RCCGGY 1 cut(s) 603
BssECI CCNNGG 1 cut(s) 429
BssMI GATC 4 cut(s) 257, 415, 617, 625
BssNAI GTATAC 1 cut(s) 345
Bst1107I GTATAC 1 cut(s) 345
Bst2UI CCWGG 1 cut(s) 430
Bst6I CTCTTC 2 cut(s) 125, 530
BstC8I GCNNGC 3 cut(s) 279, 294, 605
BstDEI CTNAG 3 cut(s) 25, 164, 528
BstF5I GGATG 6 cut(s) 54, 62, 75, 307, 493, 562
BstHHI GCGC 1 cut(s) 603
BstKTI GATC 4 cut(s) 260, 418, 620, 628
BstMAI GTCTC 1 cut(s) 374
BstMBI GATC 4 cut(s) 257, 415, 617, 625
BstMWI GCNNNNNNNGC 5 cut(s) 45, 166, 182, 452, 520
BstNI CCWGG 1 cut(s) 430
BstSCI CCNGG 5 cut(s) 155, 296, 325, 360, 428
BstSLI GKGCMC 1 cut(s) 162
BstV1I GCAGC 3 cut(s) 301, 507, 547
BstV2I GAAGAC 1 cut(s) 428
BstX2I RGATCY 1 cut(s) 257
BstYI RGATCY 1 cut(s) 257
BstZ17I GTATAC 1 cut(s) 345
Bsu15I ATCGAT 1 cut(s) 508
Bsu36I CCTNAGG 1 cut(s) 164
BsuRI GGCC 4 cut(s) 160, 182, 296, 428
BsuTUI ATCGAT 1 cut(s) 508
BtrI CACGTC 1 cut(s) 316
BtsCI GGATG 6 cut(s) 54, 62, 75, 307, 493, 562
Cac8I GCNNGC 3 cut(s) 279, 294, 605
CfoI GCGC 1 cut(s) 603
Cfr10I RCCGGY 1 cut(s) 603
Cfr13I GGNCC 3 cut(s) 158, 159, 295
ClaI ATCGAT 1 cut(s) 508
Csp6I GTAC 2 cut(s) 211, 304
CviAII CATG 3 cut(s) 424, 547, 585
CviQI GTAC 2 cut(s) 211, 304
DdeI CTNAG 3 cut(s) 25, 164, 528
DpnI GATC 4 cut(s) 259, 417, 619, 627
DpnII GATC 4 cut(s) 257, 415, 617, 625
EaeI YGGCCR 1 cut(s) 426
Eam1104I CTCTTC 2 cut(s) 125, 530
EarI CTCTTC 2 cut(s) 125, 530
EciI GGCGGA 1 cut(s) 221
Ecl136II GAGCTC 2 cut(s) 220, 593
Eco24I GRGCYC 3 cut(s) 162, 222, 595
Eco31I GGTCTC 1 cut(s) 374
Eco53kI GAGCTC 2 cut(s) 220, 593
Eco81I CCTNAGG 1 cut(s) 164
EcoICRI GAGCTC 2 cut(s) 220, 593
EcoO109I RGGNCCY 1 cut(s) 159
EcoRII CCWGG 1 cut(s) 428
EcoT38I GRGCYC 3 cut(s) 162, 222, 595
FaeI CATG 3 cut(s) 427, 550, 588
FalI AAGNNNNNCTT 2 cut(s) 391, 423
FatI CATG 3 cut(s) 423, 546, 584
FauNDI CATATG 1 cut(s) 579
FbaI TGATCA 1 cut(s) 415
FblI GTMKAC 1 cut(s) 344
Fnu4HI GCNGC 4 cut(s) 183, 290, 496, 561
FokI GGATG 6 cut(s) 41, 49, 62, 294, 500, 569
FriOI GRGCYC 3 cut(s) 162, 222, 595
Fsp4HI GCNGC 4 cut(s) 183, 290, 496, 561
GlaI GCGC 1 cut(s) 602
GluI GCNGC 4 cut(s) 183, 290, 496, 561
GsaI CCCAGC 1 cut(s) 114
GsuI CTGGAG 1 cut(s) 520
HaeIII GGCC 4 cut(s) 160, 182, 296, 428
HapII CCGG 8 cut(s) 145, 156, 255, 298, 311, 326, 361, 604
HhaI GCGC 1 cut(s) 603
Hin1II CATG 3 cut(s) 427, 550, 588
Hin6I GCGC 1 cut(s) 601
HinP1I GCGC 1 cut(s) 601
HinfI GANTC 4 cut(s) 41, 251, 392, 505
HpaII CCGG 8 cut(s) 145, 156, 255, 298, 311, 326, 361, 604
HphI GGTGA 2 cut(s) 214, 431
Hpy166II GTNNAC 1 cut(s) 345
Hpy188I TCNGA 3 cut(s) 391, 529, 625
Hpy188III TCNNGA 7 cut(s) 27, 145, 255, 311, 368, 396, 419
Hpy8I GTNNAC 1 cut(s) 345
HpyAV CCTTC 6 cut(s) 151, 172, 235, 393, 457, 608
HpyCH4IV ACGT 1 cut(s) 315
HpyCH4V TGCA 2 cut(s) 4, 455
HpyF10VI GCNNNNNNNGC 5 cut(s) 45, 166, 182, 452, 520
HpyF3I CTNAG 3 cut(s) 25, 164, 528
HpySE526I ACGT 1 cut(s) 315
Hsp92II CATG 3 cut(s) 427, 550, 588
HspAI GCGC 1 cut(s) 601
Kpn2I TCCGGA 3 cut(s) 144, 254, 310
KroI GCCGGC 1 cut(s) 603
KroNI GCCGGC 1 cut(s) 605
Ksp22I TGATCA 1 cut(s) 415
Kzo9I GATC 4 cut(s) 257, 415, 617, 625
LmnI GCTCC 2 cut(s) 217, 590
Lsp1109I GCAGC 3 cut(s) 301, 507, 547
LweI GCATC 2 cut(s) 452, 547
MaeII ACGT 1 cut(s) 315
MaeIII GTNAC 1 cut(s) 262
MalI GATC 4 cut(s) 259, 417, 619, 627
MboI GATC 4 cut(s) 257, 415, 617, 625
MboII GAAGA 4 cut(s) 112, 428, 547, 552
MflI RGATCY 1 cut(s) 257
MhlI GDGCHC 3 cut(s) 162, 222, 595
MlsI TGGCCA 1 cut(s) 428
MluNI TGGCCA 1 cut(s) 428
Mox20I TGGCCA 1 cut(s) 428
MroI TCCGGA 3 cut(s) 144, 254, 310
MroNI GCCGGC 1 cut(s) 603
MroXI GAANNNNTTC 1 cut(s) 40
MscI TGGCCA 1 cut(s) 428
Msp20I TGGCCA 1 cut(s) 428
MspA1I CMGCKG 2 cut(s) 292, 498
MspI CCGG 8 cut(s) 145, 156, 255, 298, 311, 326, 361, 604
MspR9I CCNGG 5 cut(s) 157, 298, 327, 362, 430
Mva1269I GAATGC 1 cut(s) 603
MvaI CCWGG 1 cut(s) 430
MwoI GCNNNNNNNGC 5 cut(s) 45, 166, 182, 452, 520
NaeI GCCGGC 1 cut(s) 605
NciI CCSGG 4 cut(s) 157, 298, 327, 362
NdeI CATATG 1 cut(s) 579
NdeII GATC 4 cut(s) 257, 415, 617, 625
NgoMIV GCCGGC 1 cut(s) 603
NlaIII CATG 3 cut(s) 427, 550, 588
NlaIV GGNNCC 4 cut(s) 149, 160, 161, 259
NmuCI GTSAC 1 cut(s) 262
PctI GAATGC 1 cut(s) 603
PdiI GCCGGC 1 cut(s) 605
PdmI GAANNNNTTC 1 cut(s) 40
PfeI GAWTC 4 cut(s) 41, 251, 392, 505
PflFI GACNNNGTC 1 cut(s) 385
PkrI GCNGC 4 cut(s) 184, 291, 497, 562
Psp124BI GAGCTC 2 cut(s) 222, 595
Psp6I CCWGG 1 cut(s) 428
PspFI CCCAGC 1 cut(s) 110
PspGI CCWGG 1 cut(s) 428
PspN4I GGNNCC 4 cut(s) 149, 160, 161, 259
PspOMI GGGCCC 1 cut(s) 158
PspPI GGNCC 3 cut(s) 158, 159, 295
PsuI RGATCY 1 cut(s) 257
PsyI GACNNNGTC 1 cut(s) 385
PvuII CAGCTG 2 cut(s) 292, 498
RsaI GTAC 2 cut(s) 212, 305
RsaNI GTAC 2 cut(s) 211, 304
SacI GAGCTC 2 cut(s) 222, 595
SatI GCNGC 4 cut(s) 183, 290, 496, 561
Sau3AI GATC 4 cut(s) 257, 415, 617, 625
Sau96I GGNCC 3 cut(s) 158, 159, 295
ScrFI CCNGG 5 cut(s) 157, 298, 327, 362, 430
SduI GDGCHC 3 cut(s) 162, 222, 595
SfaNI GCATC 2 cut(s) 452, 547
SsiI CCGC 2 cut(s) 183, 206
SstI GAGCTC 2 cut(s) 222, 595
StyD4I CCNGG 5 cut(s) 155, 296, 325, 360, 428
TaiI ACGT 1 cut(s) 318
TaqI TCGA 3 cut(s) 395, 508, 595
TaqII GACCGA 1 cut(s) 281
TatI WGTACW 1 cut(s) 210
TauI GCSGC 1 cut(s) 185
TfiI GAWTC 4 cut(s) 41, 251, 392, 505
TseFI GTSAC 1 cut(s) 262
TseI GCWGC 3 cut(s) 289, 495, 560
Tsp45I GTSAC 1 cut(s) 262
TspDTI ATGAA 1 cut(s) 563
TspGWI ACGGA 2 cut(s) 250, 362
Tth111I GACNNNGTC 1 cut(s) 385
XmiI GTMKAC 1 cut(s) 344
XmnI GAANNNNTTC 1 cut(s) 40
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.