Rw4G015250

Chaperone protein DNAj

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0012219
Physical Location & Seq
Reverse (-)
35016005 .. 35017788
1784 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G015250.1

Sequence Viewer

Length: 600 bp
ATGCCAAGCTACGGCTTGACCATCCCGGGAGCCGATTCCCGCCTCTACGTCACCACCATCTCCACCGCCCGACCCTTGCCCGACCCGAAAGCCAGTTCGCTGCCGCTGTTCTCGTCCCGGGCGACCCGGTTCGGGTCGATCAAGGCCAAGGCGGCGACGGTGAACGACGCGGCGGCGGAGGAGCGGAAGGAGCTGTCGTTTTACGAGCTGCTGGGGATACCCGAGTCCGGATCCATAACTGAGATCAAGCAGGCGTACAAGCAGCTGGCAAGGAAGTACCACCCGGACGTGTCGCCGCCGGGTCGGGTCGAGGAGTATACGGAGAAGTTTATTCGGGTTCAGGAGGCGTATGAGACGTTGTCGGATTCGAGGAGGAGGGCTATGTATGATCAGGACATGGCTAGGGGTCTTCACCTTGCCTTCTCTGCTAGAAGATACCAATATGATGAGGGAATGGAAGGAAAAGGTGACTGGAAGAATCGCTGGCAAAGCCAGCTATCTGAGCTGAAGAGAAGAAGCTCATTCAAGGATGATCAACAAAATATGTCATGGGGAGCTAGAATGCGTCGGCAAAGAGAGGGACTGTCTGATGAAGTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DnaJ

Protein Analysis

199

Amino Acids

22.8

Weight (kDa)

9.34

Isoelectric Point (pI)

65.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DnaJ PF00226 67 - 130 5.4e-21 DnaJ domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012219)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G13830 AT4G13830 AT4G13830
fragaria_vesca FvH4_4g13370
malus_domestica MD04G1016600.v1.1
prunus_persica Prupe.1G163300_v2.0.a1 Prupe.1G163300_v2.0.a1 Prupe.1G204200_v2.0.a1
pyrus_communis pycom04g01270
rosa_chinensis RchiOBHm_Chr4g0413741
rosa_laevigata RLG00000008197
rosa_multiflora Rmu_co8100182.1_g000001
rosa_roxburghii Rroxscaffold_5G00357660
rosa_rugosa Rorug04G0122800
rosa_samantha Rh4AG182200 Rh4BG181300 Rh4CG193400 Rh4DG177800
rosa_wichuraiana Rw4G015250

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 184
AccI GTMKAC 1 cut(s) 317
AccII CGCG 1 cut(s) 170
AccIII TCCGGA 1 cut(s) 227
AciI CCGC 9 cut(s) 40, 66, 104, 152, 170, 173, 176, 184, 296
AclWI GGATC 2 cut(s) 225, 238
AcuI CTGAAG 1 cut(s) 527
AfaI GTAC 2 cut(s) 257, 278
AfiI CCNNNNNNNGG 3 cut(s) 11, 132, 227
AflIII ACRYGT 1 cut(s) 288
AgsI TTSAA 1 cut(s) 526
AjiI CACGTC 1 cut(s) 289
AluBI AGCT 8 cut(s) 9, 193, 208, 265, 496, 505, 519, 557
AluI AGCT 8 cut(s) 9, 193, 208, 265, 496, 505, 519, 557
Alw26I GTCTC 1 cut(s) 347
AlwI GGATC 2 cut(s) 225, 238
Ama87I CYCGRG 3 cut(s) 25, 117, 221
Aor13HI TCCGGA 1 cut(s) 227
AoxI GGCC 1 cut(s) 144
ApeKI GCWGC 3 cut(s) 100, 208, 262
AsuC2I CCSGG 7 cut(s) 26, 27, 118, 119, 127, 284, 300
AsuHPI GGTGA 4 cut(s) 43, 172, 404, 479
AvaI CYCGRG 3 cut(s) 25, 117, 221
BaeI ACNNNNGTAYC 2 cut(s) 209, 242
BamHI GGATCC 1 cut(s) 230
BbsI GAAGAC 1 cut(s) 401
BbvI GCAGC 3 cut(s) 87, 195, 274
BccI CCATC 2 cut(s) 29, 65
BceAI ACGGC 1 cut(s) 28
BciVI GTATCC 1 cut(s) 210
BclI TGATCA 2 cut(s) 388, 532
BcnI CCSGG 7 cut(s) 26, 27, 118, 119, 127, 284, 300
BcoDI GTCTC 1 cut(s) 347
BfaI CTAG 3 cut(s) 402, 429, 558
BfuI GTATCC 1 cut(s) 210
BglI GCCNNNNNGGC 1 cut(s) 152
BisI GCNGC 8 cut(s) 101, 104, 153, 171, 174, 209, 263, 296
BlsI GCNGC 8 cut(s) 102, 105, 154, 172, 175, 210, 264, 297
Bme1390I CCNGG 7 cut(s) 26, 27, 118, 119, 127, 284, 300
BmeT110I CYCGRG 3 cut(s) 25, 117, 221
BmgBI CACGTC 1 cut(s) 289
BmiI GGNNCC 2 cut(s) 31, 232
BmrFI CCNGG 7 cut(s) 26, 27, 118, 119, 127, 284, 300
BpiI GAAGAC 1 cut(s) 401
BpuMI CCSGG 7 cut(s) 26, 27, 118, 119, 127, 284, 300
BsaJI CCNNGG 3 cut(s) 25, 117, 147
BsaWI WCCGGW 1 cut(s) 227
BsaXI ACNNNNNCTCC 2 cut(s) 182, 212
Bsc4I CCNNNNNNNGG 3 cut(s) 11, 132, 227
Bse1I ACTGG 2 cut(s) 93, 476
BseAI TCCGGA 1 cut(s) 227
BseDI CCNNGG 3 cut(s) 25, 117, 147
BseGI GGATG 2 cut(s) 21, 535
BseLI CCNNNNNNNGG 3 cut(s) 11, 132, 227
BseMII CTCAG 2 cut(s) 231, 492
BseNI ACTGG 2 cut(s) 93, 476
BseRI GAGGAG 4 cut(s) 194, 326, 385, 388
BseXI GCAGC 3 cut(s) 87, 195, 274
BseYI CCCAGC 1 cut(s) 211
Bsh1236I CGCG 1 cut(s) 170
BshFI GGCC 1 cut(s) 146
BsiHKCI CYCGRG 3 cut(s) 25, 117, 221
BsiSI CCGG 6 cut(s) 26, 118, 127, 228, 284, 299
BslFI GGGAC 2 cut(s) 100, 594
BslI CCNNNNNNNGG 3 cut(s) 11, 132, 227
BsmAI GTCTC 1 cut(s) 347
BsmBI CGTCTC 1 cut(s) 347
BsmFI GGGAC 2 cut(s) 100, 594
BsmI GAATGC 1 cut(s) 567
BsnI GGCC 1 cut(s) 146
BsoBI CYCGRG 3 cut(s) 25, 117, 221
Bsp13I TCCGGA 1 cut(s) 227
Bsp143I GATC 5 cut(s) 138, 230, 243, 388, 532
BspACI CCGC 9 cut(s) 40, 66, 104, 152, 170, 173, 176, 184, 296
BspANI GGCC 1 cut(s) 146
BspCNI CTCAG 2 cut(s) 232, 493
BspEI TCCGGA 1 cut(s) 227
BspFNI CGCG 1 cut(s) 170
BspLI GGNNCC 2 cut(s) 31, 232
BspPI GGATC 2 cut(s) 225, 238
BsrBI CCGCTC 1 cut(s) 184
BsrI ACTGG 2 cut(s) 93, 476
BssECI CCNNGG 3 cut(s) 25, 117, 147
BssMI GATC 5 cut(s) 138, 230, 243, 388, 532
BssNAI GTATAC 1 cut(s) 318
BssT1I CCWWGG 1 cut(s) 147
Bst1107I GTATAC 1 cut(s) 318
Bst4CI ACNGT 2 cut(s) 160, 585
Bst6I CTCTTC 1 cut(s) 503
BstC8I GCNNGC 4 cut(s) 252, 267, 485, 494
BstDEI CTNAG 2 cut(s) 240, 501
BstF5I GGATG 2 cut(s) 21, 535
BstFNI CGCG 1 cut(s) 170
BstKTI GATC 5 cut(s) 141, 233, 246, 391, 535
BstMAI GTCTC 1 cut(s) 347
BstMBI GATC 5 cut(s) 138, 230, 243, 388, 532
BstMWI GCNNNNNNNGC 6 cut(s) 152, 190, 425, 489, 493, 502
BstSCI CCNGG 7 cut(s) 24, 25, 116, 117, 125, 282, 298
BstUI CGCG 1 cut(s) 170
BstV1I GCAGC 3 cut(s) 87, 195, 274
BstV2I GAAGAC 1 cut(s) 401
BstX2I RGATCY 1 cut(s) 230
BstYI RGATCY 1 cut(s) 230
BstZ17I GTATAC 1 cut(s) 318
BsuI GTATCC 1 cut(s) 210
BsuRI GGCC 1 cut(s) 146
BtrI CACGTC 1 cut(s) 289
BtsCI GGATG 2 cut(s) 21, 535
Cac8I GCNNGC 4 cut(s) 252, 267, 485, 494
Cfr9I CCCGGG 2 cut(s) 25, 117
CseI GACGC 2 cut(s) 176, 554
Csp6I GTAC 2 cut(s) 256, 277
CviAII CATG 2 cut(s) 397, 549
CviQI GTAC 2 cut(s) 256, 277
DdeI CTNAG 2 cut(s) 240, 501
DpnI GATC 5 cut(s) 140, 232, 245, 390, 534
DpnII GATC 5 cut(s) 138, 230, 243, 388, 532
Eam1104I CTCTTC 1 cut(s) 503
EarI CTCTTC 1 cut(s) 503
EciI GGCGGA 1 cut(s) 191
Eco130I CCWWGG 1 cut(s) 147
Eco57I CTGAAG 1 cut(s) 527
Eco88I CYCGRG 3 cut(s) 25, 117, 221
EcoT14I CCWWGG 1 cut(s) 147
ErhI CCWWGG 1 cut(s) 147
Esp3I CGTCTC 1 cut(s) 347
FaeI CATG 2 cut(s) 400, 552
FaqI GGGAC 2 cut(s) 100, 594
FatI CATG 2 cut(s) 396, 548
FauI CCCGC 1 cut(s) 47
FbaI TGATCA 2 cut(s) 388, 532
FblI GTMKAC 1 cut(s) 317
Fnu4HI GCNGC 8 cut(s) 101, 104, 153, 171, 174, 209, 263, 296
FokI GGATG 2 cut(s) 8, 542
Fsp4HI GCNGC 8 cut(s) 101, 104, 153, 171, 174, 209, 263, 296
FspBI CTAG 3 cut(s) 402, 429, 558
GluI GCNGC 8 cut(s) 101, 104, 153, 171, 174, 209, 263, 296
GsaI CCCAGC 1 cut(s) 215
HaeIII GGCC 1 cut(s) 146
HapII CCGG 6 cut(s) 26, 118, 127, 228, 284, 299
HgaI GACGC 2 cut(s) 176, 554
Hin1II CATG 2 cut(s) 400, 552
HinfI GANTC 4 cut(s) 35, 224, 365, 478
HpaII CCGG 6 cut(s) 26, 118, 127, 228, 284, 299
HphI GGTGA 4 cut(s) 43, 172, 404, 479
Hpy166II GTNNAC 2 cut(s) 163, 318
Hpy188I TCNGA 3 cut(s) 364, 502, 589
Hpy188III TCNNGA 3 cut(s) 228, 341, 392
Hpy8I GTNNAC 2 cut(s) 163, 318
Hpy99I CGWCG 3 cut(s) 160, 170, 570
HpyAV CCTTC 3 cut(s) 181, 430, 452
HpyCH4III ACNGT 2 cut(s) 160, 585
HpyCH4IV ACGT 3 cut(s) 48, 288, 356
HpyF10VI GCNNNNNNNGC 6 cut(s) 152, 190, 425, 489, 493, 502
HpyF3I CTNAG 2 cut(s) 240, 501
HpySE526I ACGT 3 cut(s) 48, 288, 356
Hsp92II CATG 2 cut(s) 400, 552
Kpn2I TCCGGA 1 cut(s) 227
Ksp22I TGATCA 2 cut(s) 388, 532
Kzo9I GATC 5 cut(s) 138, 230, 243, 388, 532
LmnI GCTCC 4 cut(s) 29, 181, 190, 554
Lsp1109I GCAGC 3 cut(s) 87, 195, 274
MaeI CTAG 3 cut(s) 402, 429, 558
MaeII ACGT 3 cut(s) 48, 288, 356
MaeIII GTNAC 2 cut(s) 49, 467
MalI GATC 5 cut(s) 140, 232, 245, 390, 534
MbiI CCGCTC 1 cut(s) 184
MboI GATC 5 cut(s) 138, 230, 243, 388, 532
MboII GAAGA 5 cut(s) 401, 444, 487, 520, 525
MflI RGATCY 1 cut(s) 230
MlyI GAGTC 1 cut(s) 233
MmeI TCCRAC 1 cut(s) 342
MnlI CCTC 9 cut(s) 53, 172, 304, 337, 363, 366, 369, 442, 571
MroI TCCGGA 1 cut(s) 227
MspA1I CMGCKG 2 cut(s) 106, 265
MspI CCGG 6 cut(s) 26, 118, 127, 228, 284, 299
MspR9I CCNGG 7 cut(s) 26, 27, 118, 119, 127, 284, 300
Mva1269I GAATGC 1 cut(s) 567
MvnI CGCG 1 cut(s) 170
MwoI GCNNNNNNNGC 6 cut(s) 152, 190, 425, 489, 493, 502
NciI CCSGG 7 cut(s) 26, 27, 118, 119, 127, 284, 300
NdeII GATC 5 cut(s) 138, 230, 243, 388, 532
NlaIII CATG 2 cut(s) 400, 552
NlaIV GGNNCC 2 cut(s) 31, 232
NmuCI GTSAC 2 cut(s) 49, 467
PcsI WCGNNNNNNNCGW 1 cut(s) 119
PctI GAATGC 1 cut(s) 567
PfeI GAWTC 3 cut(s) 35, 365, 478
PflFI GACNNNGTC 1 cut(s) 358
PkrI GCNGC 8 cut(s) 102, 105, 154, 172, 175, 210, 264, 297
PleI GAGTC 1 cut(s) 232
PpsI GAGTC 1 cut(s) 232
PspFI CCCAGC 1 cut(s) 211
PspN4I GGNNCC 2 cut(s) 31, 232
PsuI RGATCY 1 cut(s) 230
PsyI GACNNNGTC 1 cut(s) 358
PvuII CAGCTG 1 cut(s) 265
RsaI GTAC 2 cut(s) 257, 278
RsaNI GTAC 2 cut(s) 256, 277
SatI GCNGC 8 cut(s) 101, 104, 153, 171, 174, 209, 263, 296
Sau3AI GATC 5 cut(s) 138, 230, 243, 388, 532
SchI GAGTC 1 cut(s) 233
ScrFI CCNGG 7 cut(s) 26, 27, 118, 119, 127, 284, 300
SmaI CCCGGG 2 cut(s) 27, 119
SsiI CCGC 9 cut(s) 40, 66, 104, 152, 170, 173, 176, 184, 296
SspMI CTAG 3 cut(s) 402, 429, 558
StyD4I CCNGG 7 cut(s) 24, 25, 116, 117, 125, 282, 298
StyI CCWWGG 1 cut(s) 147
TaaI ACNGT 2 cut(s) 160, 585
TaiI ACGT 3 cut(s) 51, 291, 359
TaqI TCGA 3 cut(s) 137, 309, 368
TauI GCSGC 5 cut(s) 106, 155, 173, 176, 298
TfiI GAWTC 3 cut(s) 35, 365, 478
TseFI GTSAC 2 cut(s) 49, 467
TseI GCWGC 3 cut(s) 100, 208, 262
Tsp45I GTSAC 2 cut(s) 49, 467
TspGWI ACGGA 1 cut(s) 335
TspMI CCCGGG 2 cut(s) 25, 117
Tth111I GACNNNGTC 1 cut(s) 358
XmaI CCCGGG 2 cut(s) 25, 117
XmiI GTMKAC 1 cut(s) 317
XspI CTAG 3 cut(s) 402, 429, 558
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.