Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr04

Orthogroup ID

OG0014358

Physical Location & Seq

Genomic Coordinates Reverse (-)

3443970 .. 3450951

Gene Structure 6982 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD04G1030700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 864 bp

ATGGCTGCTCCGATGAAGATTGTATTCGGGCTGCTAACCTTTGTCACTGTCGGAATGATCATAGGTGCTTTGTTTCAATTGGCATTTATACGAAGATTAGAAGACTCATACGGCTCGGGGTTTCCATCTCCTAGAAAAGTGCTTGGAAGTGATGGTTATCGTCAGTTGCCCGGAGGTGCTTATTGGAGCAATGATAAAGAAGCAGAAATTTTACGCCTGGGATATGTCAAACCTGAAGTAATCAGCTGGGAGCCTCGAATAATTGTACTTCATAAGTTCTTGAGCATGGAGGGTGATCGTGAATGTGACTACCTTAGAACTCTTGCCTCCCCTCGCCTTCAAGTCTCCACTGTAGTGGACGTAAGAACAGGAAAGGGAATAAAGAGTAATGTTCGCACAAGTTCTGGTATGTTCTTGAGTCCCGAAGAGAAAAAGTACCCGATGATACAGGCAATTGAAAAGCGTATTGCTGTGTTTTCTCAAGTGCCAATAGAAAATGGGGAGCTCATTCAAGTTTTAAGGTACGAAAAGGATCAGTATTACAGACCTCACCATGACTACTTCTCTGACACTTTTAACTTGAAACGTGGTGGTCAGCGAGTAGCAACAATTCTGATGTATTTGAGTGATAATGTTGAGGGCGGAGAAACCATCTTCCCTACGGCTAGTTCAGGTAAATGTACCTGCGGTGGAAGAGCTGCCACAGGTTTGTCTGTGAAGCCAATTAAAGGGGATGCAGTACTTTTTTGGAGCATGAATCTTGAAGGAAAAGAGGATCCGACGAGCATACATGGAGGATGTGAGGTACTATCCGGGGAAAAGTGGTCGGCTACAAAATGGATGAGGCAAAAACGTACCTCTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004656 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005794 GO:0006082 GO:0006464 GO:0006520 GO:0006575 GO:0006807 GO:0008150 GO:0008152 GO:0009987 GO:0012505 GO:0016491 GO:0016705 GO:0016706 GO:0018126 GO:0018193 GO:0018208 GO:0018401 GO:0019471 GO:0019511 GO:0019538 GO:0019752 GO:0019798 GO:0031543 GO:0031545 GO:0036211 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043436 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044281 GO:0044424 GO:0044444 GO:0044464 GO:0051213 GO:0055114 GO:0071704 GO:0140096 GO:1901564 GO:1901605

KEGG Pathways

Metabolic & Signaling

ko00330 ko01100 map00330 map01100

Pfam Domains

Protein Families

2OG-FeII_Oxy_3

Protein Analysis

288

Amino Acids

32.09

Weight (kDa)

9.14

Isoelectric Point (pI)

36.31

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
2OG-FeII_Oxy	PF03171	169 - 282	1.9e-09	2OG-Fe(II) oxygenase superfamily
2OG-FeII_Oxy_3	PF13640	170 - 282	3.1e-18	2OG-Fe(II) oxygenase superfamily

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Publication-ready

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Orthologous Genes (Group: OG0014358)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G43080
fragaria_vesca	FvH4_4g11880 FvH4_4g11880 FvH4_4g11880
malus_domestica	MD04G1030700.v1.1
prunus_persica	Prupe.1G180100_v2.0.a1 Prupe.1G180100_v2.0.a1
pyrus_communis	pycom04g02600
rosa_chinensis	RchiOBHm_Chr4g0409351
rosa_laevigata	RLG00000008567
rosa_multiflora	Rmu_sc0001616.1_g000050
rosa_roxburghii	Rroxscaffold_5G00353870
rosa_rugosa	Rorug04G0093100
rosa_samantha	Rh4AG155700 Rh4BG154200 Rh4DG148700
rosa_wichuraiana	Rw4G012740

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	692
AciI	CCGC	2 cut(s)	642, 687
AclWI	GGATC	3 cut(s)	540, 770, 783
AcsI	RAATTY	1 cut(s)	207
AcuI	CTGAAG	1 cut(s)	255
AfaI	GTAC	7 cut(s)	267, 437, 524, 682, 741, 807, 856
AfiI	CCNNNNNNNGG	1 cut(s)	728
AgsI	TTSAA	6 cut(s)	77, 341, 458, 512, 583, 764
AjnI	CCWGG	1 cut(s)	216
AleI	CACNNNNGTG	1 cut(s)	353
AluBI	AGCT	3 cut(s)	246, 505, 698
AluI	AGCT	3 cut(s)	246, 505, 698
Alw21I	GWGCWC	1 cut(s)	507
Alw26I	GTCTC	1 cut(s)	349
AlwI	GGATC	3 cut(s)	540, 770, 783
Ama87I	CYCGRG	1 cut(s)	115
ApeKI	GCWGC	3 cut(s)	5, 31, 698
ApoI	RAATTY	1 cut(s)	207
AsuC2I	CCSGG	2 cut(s)	171, 814
AsuHPI	GGTGA	2 cut(s)	305, 542
AvaI	CYCGRG	1 cut(s)	115
BamHI	GGATCC	1 cut(s)	775
BanII	GRGCYC	1 cut(s)	507
BbsI	GAAGAC	1 cut(s)	108
Bbv12I	GWGCWC	1 cut(s)	507
BbvI	GCAGC	2 cut(s)	18, 685
BccI	CCATC	3 cut(s)	133, 146, 659
BceAI	ACGGC	2 cut(s)	127, 678
BciT130I	CCWGG	1 cut(s)	218
BclI	TGATCA	1 cut(s)	57
BcnI	CCSGG	2 cut(s)	171, 814
BcoDI	GTCTC	1 cut(s)	349
BfaI	CTAG	2 cut(s)	132, 666
BfmI	CTRYAG	1 cut(s)	351
BfuAI	ACCTGC	1 cut(s)	692
BisI	GCNGC	3 cut(s)	6, 32, 699
BlsI	GCNGC	3 cut(s)	7, 33, 700
BmcAI	AGTACT	1 cut(s)	741
Bme1390I	CCNGG	3 cut(s)	171, 218, 814
BmeT110I	CYCGRG	1 cut(s)	115
BmiI	GGNNCC	2 cut(s)	252, 777
BmrFI	CCNGG	3 cut(s)	171, 218, 814
BmsI	GCATC	1 cut(s)	724
BpiI	GAAGAC	1 cut(s)	108
BpuEI	CTTGAG	3 cut(s)	301, 436, 465
BpuMI	CCSGG	2 cut(s)	171, 814
BsaBI	GATNNNNATC	1 cut(s)	156
BsaJI	CCNNGG	2 cut(s)	217, 813
Bsc4I	CCNNNNNNNGG	1 cut(s)	728
Bse3DI	GCAATG	1 cut(s)	196
Bse8I	GATNNNNATC	1 cut(s)	156
BseBI	CCWGG	1 cut(s)	218
BseDI	CCNNGG	2 cut(s)	217, 813
BseGI	GGATG	3 cut(s)	739, 803, 846
BseJI	GATNNNNATC	1 cut(s)	156
BseLI	CCNNNNNNNGG	1 cut(s)	728
BseMI	GCAATG	1 cut(s)	196
BseXI	GCAGC	2 cut(s)	18, 685
BseYI	CCCAGC	1 cut(s)	246
BsiHKAI	GWGCWC	1 cut(s)	507
BsiHKCI	CYCGRG	1 cut(s)	115
BsiSI	CCGG	2 cut(s)	171, 813
BslFI	GGGAC	1 cut(s)	405
BslI	CCNNNNNNNGG	1 cut(s)	728
BsmAI	GTCTC	1 cut(s)	349
BsmFI	GGGAC	1 cut(s)	405
BsoBI	CYCGRG	1 cut(s)	115
Bsp1286I	GDGCHC	1 cut(s)	507
Bsp143I	GATC	4 cut(s)	57, 295, 532, 775
BspACI	CCGC	2 cut(s)	642, 687
BspLI	GGNNCC	2 cut(s)	252, 777
BspMI	ACCTGC	1 cut(s)	692
BspPI	GGATC	3 cut(s)	540, 770, 783
BspQI	GCTCTTC	1 cut(s)	688
BsrDI	GCAATG	1 cut(s)	196
BssECI	CCNNGG	2 cut(s)	217, 813
BssMI	GATC	4 cut(s)	57, 295, 532, 775
Bst2UI	CCWGG	1 cut(s)	218
Bst4CI	ACNGT	2 cut(s)	49, 352
Bst6I	CTCTTC	2 cut(s)	420, 688
BstDEI	CTNAG	2 cut(s)	314, 861
BstF5I	GGATG	3 cut(s)	739, 803, 846
BstKTI	GATC	4 cut(s)	60, 298, 535, 778
BstMAI	GTCTC	1 cut(s)	349
BstMBI	GATC	4 cut(s)	57, 295, 532, 775
BstNI	CCWGG	1 cut(s)	218
BstSCI	CCNGG	3 cut(s)	169, 216, 812
BstSFI	CTRYAG	1 cut(s)	351
BstV1I	GCAGC	2 cut(s)	18, 685
BstV2I	GAAGAC	1 cut(s)	108
BstX2I	RGATCY	1 cut(s)	775
BstXI	CCANNNNNNTGG	1 cut(s)	355
BstYI	RGATCY	1 cut(s)	775
BtsCI	GGATG	3 cut(s)	739, 803, 846
BtsIMutI	CAGTG	2 cut(s)	45, 348
BveI	ACCTGC	1 cut(s)	692
Csp6I	GTAC	7 cut(s)	266, 436, 523, 681, 740, 806, 855
CviAII	CATG	4 cut(s)	286, 554, 754, 791
CviQI	GTAC	7 cut(s)	266, 436, 523, 681, 740, 806, 855
DdeI	CTNAG	2 cut(s)	314, 861
DpnI	GATC	4 cut(s)	59, 297, 534, 777
DpnII	GATC	4 cut(s)	57, 295, 532, 775
Eam1104I	CTCTTC	2 cut(s)	420, 688
EarI	CTCTTC	2 cut(s)	420, 688
EciI	GGCGGA	1 cut(s)	657
Ecl136II	GAGCTC	1 cut(s)	505
Eco24I	GRGCYC	1 cut(s)	507
Eco53kI	GAGCTC	1 cut(s)	505
Eco57I	CTGAAG	1 cut(s)	255
Eco88I	CYCGRG	1 cut(s)	115
EcoICRI	GAGCTC	1 cut(s)	505
EcoRII	CCWGG	1 cut(s)	216
EcoT38I	GRGCYC	1 cut(s)	507
FaeI	CATG	4 cut(s)	289, 557, 757, 794
FaqI	GGGAC	1 cut(s)	405
FatI	CATG	4 cut(s)	285, 553, 753, 790
FbaI	TGATCA	1 cut(s)	57
Fnu4HI	GCNGC	3 cut(s)	6, 32, 699
FokI	GGATG	3 cut(s)	746, 810, 853
FriOI	GRGCYC	1 cut(s)	507
Fsp4HI	GCNGC	3 cut(s)	6, 32, 699
FspBI	CTAG	2 cut(s)	132, 666
GluI	GCNGC	3 cut(s)	6, 32, 699
GsaI	CCCAGC	1 cut(s)	250
HapII	CCGG	2 cut(s)	171, 813
Hin1II	CATG	4 cut(s)	289, 557, 757, 794
HinfI	GANTC	3 cut(s)	104, 418, 757
HpaII	CCGG	2 cut(s)	171, 813
HphI	GGTGA	2 cut(s)	305, 542
Hpy166II	GTNNAC	1 cut(s)	358
Hpy188I	TCNGA	5 cut(s)	12, 53, 568, 615, 780
Hpy188III	TCNNGA	5 cut(s)	280, 299, 415, 422, 761
Hpy8I	GTNNAC	1 cut(s)	358
Hpy99I	CGWCG	1 cut(s)	784
HpyAV	CCTTC	2 cut(s)	347, 758
HpyCH4III	ACNGT	2 cut(s)	49, 352
HpyCH4IV	ACGT	3 cut(s)	360, 586, 853
HpyCH4V	TGCA	1 cut(s)	737
HpyF3I	CTNAG	2 cut(s)	314, 861
HpySE526I	ACGT	3 cut(s)	360, 586, 853
Hsp92II	CATG	4 cut(s)	289, 557, 757, 794
Ksp22I	TGATCA	1 cut(s)	57
Kzo9I	GATC	4 cut(s)	57, 295, 532, 775
LguI	GCTCTTC	1 cut(s)	688
LmnI	GCTCC	5 cut(s)	13, 186, 250, 502, 750
Lsp1109I	GCAGC	2 cut(s)	18, 685
LweI	GCATC	1 cut(s)	724
MaeI	CTAG	2 cut(s)	132, 666
MaeII	ACGT	3 cut(s)	360, 586, 853
MaeIII	GTNAC	2 cut(s)	43, 305
MalI	GATC	4 cut(s)	59, 297, 534, 777
MboI	GATC	4 cut(s)	57, 295, 532, 775
MboII	GAAGA	6 cut(s)	28, 105, 113, 437, 646, 705
MfeI	CAATTG	2 cut(s)	77, 453
MflI	RGATCY	1 cut(s)	775
MhlI	GDGCHC	1 cut(s)	507
MluCI	AATT	6 cut(s)	77, 207, 261, 453, 609, 723
MlyI	GAGTC	2 cut(s)	98, 427
MmeI	TCCRAC	2 cut(s)	31, 803
MseI	TTAA	3 cut(s)	518, 576, 726
MslI	CAYNNNNRTG	1 cut(s)	353
MspA1I	CMGCKG	1 cut(s)	246
MspI	CCGG	2 cut(s)	171, 813
MspR9I	CCNGG	3 cut(s)	171, 218, 814
MunI	CAATTG	2 cut(s)	77, 453
MvaI	CCWGG	1 cut(s)	218
NciI	CCSGG	2 cut(s)	171, 814
NdeII	GATC	4 cut(s)	57, 295, 532, 775
NlaIII	CATG	4 cut(s)	289, 557, 757, 794
NlaIV	GGNNCC	2 cut(s)	252, 777
NmuCI	GTSAC	2 cut(s)	43, 305
OliI	CACNNNNGTG	1 cut(s)	353
PciSI	GCTCTTC	1 cut(s)	688
PfeI	GAWTC	1 cut(s)	757
PkrI	GCNGC	3 cut(s)	7, 33, 700
PleI	GAGTC	2 cut(s)	98, 426
PpsI	GAGTC	2 cut(s)	98, 426
Psp124BI	GAGCTC	1 cut(s)	507
Psp6I	CCWGG	1 cut(s)	216
PspFI	CCCAGC	1 cut(s)	246
PspGI	CCWGG	1 cut(s)	216
PspN4I	GGNNCC	2 cut(s)	252, 777
PsuI	RGATCY	1 cut(s)	775
PvuII	CAGCTG	1 cut(s)	246
RsaI	GTAC	7 cut(s)	267, 437, 524, 682, 741, 807, 856
RsaNI	GTAC	7 cut(s)	266, 436, 523, 681, 740, 806, 855
RseI	CAYNNNNRTG	1 cut(s)	353
SacI	GAGCTC	1 cut(s)	507
SapI	GCTCTTC	1 cut(s)	688
SaqAI	TTAA	3 cut(s)	518, 576, 726
SatI	GCNGC	3 cut(s)	6, 32, 699
Sau3AI	GATC	4 cut(s)	57, 295, 532, 775
ScaI	AGTACT	1 cut(s)	741
SchI	GAGTC	2 cut(s)	98, 427
ScrFI	CCNGG	3 cut(s)	171, 218, 814
SduI	GDGCHC	1 cut(s)	507
SfaNI	GCATC	1 cut(s)	724
SfcI	CTRYAG	1 cut(s)	351
SmiMI	CAYNNNNRTG	1 cut(s)	353
SmlI	CTYRAG	3 cut(s)	280, 415, 480
SmoI	CTYRAG	3 cut(s)	280, 415, 480
Sse9I	AATT	6 cut(s)	77, 207, 261, 453, 609, 723
SsiI	CCGC	2 cut(s)	642, 687
SspMI	CTAG	2 cut(s)	132, 666
SstI	GAGCTC	1 cut(s)	507
StyD4I	CCNGG	3 cut(s)	169, 216, 812
TaaI	ACNGT	2 cut(s)	49, 352
TaiI	ACGT	3 cut(s)	363, 589, 856
TaqI	TCGA	1 cut(s)	256
TasI	AATT	6 cut(s)	77, 207, 261, 453, 609, 723
TatI	WGTACW	2 cut(s)	265, 739
TfiI	GAWTC	1 cut(s)	757
Tru1I	TTAA	3 cut(s)	518, 576, 726
Tru9I	TTAA	3 cut(s)	518, 576, 726
TscAI	CASTG	2 cut(s)	52, 355
TseFI	GTSAC	2 cut(s)	43, 305
TseI	GCWGC	3 cut(s)	5, 31, 698
Tsp45I	GTSAC	2 cut(s)	43, 305
TspDTI	ATGAA	3 cut(s)	29, 260, 770
TspRI	CASTG	2 cut(s)	52, 355
XapI	RAATTY	1 cut(s)	207
XspI	CTAG	2 cut(s)	132, 666
ZrmI	AGTACT	1 cut(s)	741

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.