Rh4BG154200

prolyl 4-hydroxylase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0014358
Physical Location & Seq
Forward (+)
27738370 .. 27747697
9328 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG154200.1

Sequence Viewer

Length: 777 bp
ATGGCTGCTGCGATGAAGATTGTGTTCGGACTCCTTACGTTTGTCACTGTCGGAATGATTATAGGTGCTTTGTTCCAGTTGGCATTTATACGAAGATTGGAAGAGTCGTATGGATCTGAGTTTCCAGCTTCAAGGAGACTGGGCAGAAGTCTAAATGATGGCTATCTTAAATTCCCCAGAGGTGTTCCTCATTGGACCAATGACAAAGAAGCAGAAATTTTACGCCTTGGATATGTCAAACCAGAAGTGATAAGCTGGTTACCTCGAATAATTGTGCTTCATAACTTCTTGAGCATGGAGGAATGCGACTATCTTAGAGCTATTGCCTCTCCTCGCCTTGAAGTATCCACTGTAGTGGACATCAAAACAGGAAAGGGGATCAAGAGTAAGGTTCGTACCAGCTCTGGTATGTTCTTGAGTCCTCAAGAGAAAAAGTTCCCAATGATACAGGCAATTGAAAAACGTATTTCTGTCTATTCTCAAGTACCAATAGAAAATGGGGAACTGATTCAAGTTTTGAGGTATGAAAAGGACCAGTATTATAAACCTCATCACGACTACTTCTCTGACACTTTTAACTTGAAACGTGGTGGTCAGCGAGTAGCAACAATTCTGATGTATCTGAGTGACAATGTTGAGGGTGGAGAAACTTATTTTCCCATGGCTGGCTCAGGTGAATGTAGCTGTGGTGGAAAAGTTGTCAAGGGTATGTCTGTCAAGCCAACTAAAGGAGATGCTGTACTTTTCTGGAGCATGGTTAGTTTCCCTTTCAAGTGA

Protein Analysis

258

Amino Acids

29.12

Weight (kDa)

9.3

Isoelectric Point (pI)

41.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
2OG-FeII_Oxy_3 PF13640 170 - 251 5.8e-12 2OG-Fe(II) oxygenase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014358)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G43080
fragaria_vesca FvH4_4g11880 FvH4_4g11880 FvH4_4g11880
malus_domestica MD04G1030700.v1.1
prunus_persica Prupe.1G180100_v2.0.a1 Prupe.1G180100_v2.0.a1
pyrus_communis pycom04g02600
rosa_chinensis RchiOBHm_Chr4g0409351
rosa_laevigata RLG00000008567
rosa_multiflora Rmu_sc0001616.1_g000050
rosa_roxburghii Rroxscaffold_5G00353870
rosa_rugosa Rorug04G0093100
rosa_samantha Rh4AG155700 Rh4BG154200 Rh4DG148700
rosa_wichuraiana Rw4G012740

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 543
AclWI GGATC 2 cut(s) 121, 386
AcsI RAATTY 2 cut(s) 170, 216
AfaI GTAC 3 cut(s) 397, 486, 741
AfiI CCNNNNNNNGG 2 cut(s) 665, 728
AgsI TTSAA 6 cut(s) 132, 341, 458, 512, 583, 772
AleI CACNNNNGTG 1 cut(s) 353
AluBI AGCT 5 cut(s) 128, 255, 320, 402, 684
AluI AGCT 5 cut(s) 128, 255, 320, 402, 684
Alw26I GTCTC 1 cut(s) 130
AlwI GGATC 2 cut(s) 121, 386
ApeKI GCWGC 2 cut(s) 5, 8
ApoI RAATTY 2 cut(s) 170, 216
Asp700I GAANNNNTTC 2 cut(s) 434, 507
AspS9I GGNCC 2 cut(s) 195, 532
AsuHPI GGTGA 1 cut(s) 686
AvaII GGWCC 2 cut(s) 195, 532
BccI CCATC 1 cut(s) 152
BciVI GTATCC 1 cut(s) 355
BcoDI GTCTC 1 cut(s) 130
BfmI CTRYAG 1 cut(s) 351
BfuI GTATCC 1 cut(s) 355
BisI GCNGC 2 cut(s) 6, 9
BlsI GCNGC 2 cut(s) 7, 10
Bme18I GGWCC 2 cut(s) 195, 532
BmgT120I GGNCC 2 cut(s) 195, 532
BmrI ACTGGG 1 cut(s) 149
BmsI GCATC 1 cut(s) 724
BmuI ACTGGG 1 cut(s) 149
BpmI CTGGAG 1 cut(s) 769
Bpu10I CCTNAGC 1 cut(s) 670
BpuEI CTTGAG 4 cut(s) 310, 408, 436, 465
BsaBI GATNNNNATC 1 cut(s) 162
BsaJI CCNNGG 2 cut(s) 226, 660
BsaXI ACNNNNNCTCC 2 cut(s) 723, 753
Bsc4I CCNNNNNNNGG 2 cut(s) 665, 728
Bse1I ACTGG 3 cut(s) 76, 144, 535
Bse8I GATNNNNATC 1 cut(s) 162
BseDI CCNNGG 2 cut(s) 226, 660
BseJI GATNNNNATC 1 cut(s) 162
BseLI CCNNNNNNNGG 2 cut(s) 665, 728
BseMII CTCAG 3 cut(s) 108, 614, 684
BseNI ACTGG 3 cut(s) 76, 144, 535
BseRI GAGGAG 1 cut(s) 321
BslI CCNNNNNNNGG 2 cut(s) 665, 728
BsmAI GTCTC 1 cut(s) 130
BsmI GAATGC 1 cut(s) 308
Bsp143I GATC 2 cut(s) 113, 378
Bsp19I CCATGG 1 cut(s) 660
BspCNI CTCAG 3 cut(s) 109, 615, 683
BspPI GGATC 2 cut(s) 121, 386
BsrI ACTGG 3 cut(s) 76, 144, 535
BssECI CCNNGG 2 cut(s) 226, 660
BssMI GATC 2 cut(s) 113, 378
BssT1I CCWWGG 2 cut(s) 226, 660
Bst4CI ACNGT 2 cut(s) 49, 352
Bst6I CTCTTC 1 cut(s) 96
BstC8I GCNNGC 1 cut(s) 667
BstDEI CTNAG 4 cut(s) 117, 314, 623, 670
BstDSI CCRYGG 1 cut(s) 660
BstEII GGTNACC 1 cut(s) 258
BstKTI GATC 2 cut(s) 116, 381
BstMAI GTCTC 1 cut(s) 130
BstMBI GATC 2 cut(s) 113, 378
BstPI GGTNACC 1 cut(s) 258
BstSFI CTRYAG 1 cut(s) 351
BstX2I RGATCY 1 cut(s) 113
BstXI CCANNNNNNTGG 1 cut(s) 355
BstYI RGATCY 1 cut(s) 113
BsuI GTATCC 1 cut(s) 355
BtgI CCRYGG 1 cut(s) 660
BtgZI GCGATG 1 cut(s) 26
BtsIMutI CAGTG 2 cut(s) 45, 348
Cac8I GCNNGC 1 cut(s) 667
Cfr13I GGNCC 2 cut(s) 195, 532
Csp6I GTAC 3 cut(s) 396, 485, 740
CviAII CATG 3 cut(s) 295, 661, 754
CviQI GTAC 3 cut(s) 396, 485, 740
DdeI CTNAG 4 cut(s) 117, 314, 623, 670
DpnI GATC 2 cut(s) 115, 380
DpnII GATC 2 cut(s) 113, 378
Eam1104I CTCTTC 1 cut(s) 96
EarI CTCTTC 1 cut(s) 96
Eco130I CCWWGG 2 cut(s) 226, 660
Eco47I GGWCC 2 cut(s) 195, 532
Eco91I GGTNACC 1 cut(s) 258
EcoO65I GGTNACC 1 cut(s) 258
EcoT14I CCWWGG 2 cut(s) 226, 660
ErhI CCWWGG 2 cut(s) 226, 660
FaeI CATG 3 cut(s) 298, 664, 757
FatI CATG 3 cut(s) 294, 660, 753
Fnu4HI GCNGC 2 cut(s) 6, 9
Fsp4HI GCNGC 2 cut(s) 6, 9
GluI GCNGC 2 cut(s) 6, 9
GsuI CTGGAG 1 cut(s) 769
Hin1II CATG 3 cut(s) 298, 664, 757
HinfI GANTC 4 cut(s) 30, 104, 418, 508
HphI GGTGA 1 cut(s) 686
Hpy166II GTNNAC 1 cut(s) 358
Hpy188I TCNGA 6 cut(s) 29, 53, 118, 568, 615, 624
Hpy188III TCNNGA 6 cut(s) 289, 382, 415, 425, 554, 748
Hpy8I GTNNAC 1 cut(s) 358
HpyCH4III ACNGT 2 cut(s) 49, 352
HpyCH4IV ACGT 3 cut(s) 38, 463, 586
HpyF3I CTNAG 4 cut(s) 117, 314, 623, 670
HpySE526I ACGT 3 cut(s) 38, 463, 586
Hsp92II CATG 3 cut(s) 298, 664, 757
Kzo9I GATC 2 cut(s) 113, 378
LmnI GCTCC 1 cut(s) 750
LweI GCATC 1 cut(s) 724
MaeII ACGT 3 cut(s) 38, 463, 586
MaeIII GTNAC 3 cut(s) 43, 258, 626
MalI GATC 2 cut(s) 115, 380
MboI GATC 2 cut(s) 113, 378
MboII GAAGA 3 cut(s) 28, 105, 113
MfeI CAATTG 1 cut(s) 453
MflI RGATCY 1 cut(s) 113
MluCI AATT 5 cut(s) 170, 216, 270, 453, 609
MlyI GAGTC 3 cut(s) 24, 113, 427
MmeI TCCRAC 1 cut(s) 31
MroXI GAANNNNTTC 2 cut(s) 434, 507
MseI TTAA 2 cut(s) 168, 576
MslI CAYNNNNRTG 1 cut(s) 353
MunI CAATTG 1 cut(s) 453
Mva1269I GAATGC 1 cut(s) 308
NcoI CCATGG 1 cut(s) 660
NdeII GATC 2 cut(s) 113, 378
NlaIII CATG 3 cut(s) 298, 664, 757
NmuCI GTSAC 2 cut(s) 43, 626
OliI CACNNNNGTG 1 cut(s) 353
PctI GAATGC 1 cut(s) 308
PdmI GAANNNNTTC 2 cut(s) 434, 507
PfeI GAWTC 1 cut(s) 508
PkrI GCNGC 2 cut(s) 7, 10
PleI GAGTC 3 cut(s) 24, 112, 426
PpsI GAGTC 3 cut(s) 24, 112, 426
PsiI TTATAA 1 cut(s) 543
PspEI GGTNACC 1 cut(s) 258
PspPI GGNCC 2 cut(s) 195, 532
PsuI RGATCY 1 cut(s) 113
RsaI GTAC 3 cut(s) 397, 486, 741
RsaNI GTAC 3 cut(s) 396, 485, 740
RseI CAYNNNNRTG 1 cut(s) 353
SaqAI TTAA 2 cut(s) 168, 576
SatI GCNGC 2 cut(s) 6, 9
Sau3AI GATC 2 cut(s) 113, 378
Sau96I GGNCC 2 cut(s) 195, 532
SchI GAGTC 3 cut(s) 24, 113, 427
SfaNI GCATC 1 cut(s) 724
SfcI CTRYAG 1 cut(s) 351
SinI GGWCC 2 cut(s) 195, 532
SmiMI CAYNNNNRTG 1 cut(s) 353
SmlI CTYRAG 4 cut(s) 289, 415, 423, 480
SmoI CTYRAG 4 cut(s) 289, 415, 423, 480
Sse9I AATT 5 cut(s) 170, 216, 270, 453, 609
StyI CCWWGG 2 cut(s) 226, 660
TaaI ACNGT 2 cut(s) 49, 352
TaiI ACGT 3 cut(s) 41, 466, 589
TaqI TCGA 1 cut(s) 265
TasI AATT 5 cut(s) 170, 216, 270, 453, 609
TatI WGTACW 1 cut(s) 739
TfiI GAWTC 1 cut(s) 508
Tru1I TTAA 2 cut(s) 168, 576
Tru9I TTAA 2 cut(s) 168, 576
TscAI CASTG 2 cut(s) 52, 355
TseFI GTSAC 2 cut(s) 43, 626
TseI GCWGC 2 cut(s) 5, 8
Tsp45I GTSAC 2 cut(s) 43, 626
TspDTI ATGAA 3 cut(s) 29, 269, 540
TspRI CASTG 2 cut(s) 52, 355
VpaK11BI GGWCC 2 cut(s) 195, 532
XapI RAATTY 2 cut(s) 170, 216
XmnI GAANNNNTTC 2 cut(s) 434, 507
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.