MD04G1047100.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0017197
Physical Location & Seq
Forward (+)
5458696 .. 5460255
1560 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1047100.v1.1.491

Sequence Viewer

Length: 501 bp
ATGGGAATCTCGGCCTCAAAGCGAGTCAAATCCTCGCTATCCAACTCGCCCGCCTTCGACTCGGCCTGCGACTCGGCCTTCACTCACTGCCTCTCCCTAACCGAGCACGCTTTCGACGGCGTCTTCCCCTACCAGCTCCCCACAGCCTCCGATCACCTCCACAAAACCCTCCACACCCTCCACCCGCACCCTCTCATCCTCAACTGGGTCCCATCTCCCCCGACTCGCTCCCTTGTCGACTCCGCCCTCAAGGCCGTCACTCGTCCCGCCCACGCCCAAACTCTTGGGCCGGCCCAGTTCAAGCAGTGGGCAGTAGAGCTCTTCGCCGGCGCTGTCGTCGAGAATGCTGGGAAGGCCGTGATGTTTCGAGTTCCGATCGGCGTCGCCGGGATTGCTGGGATCGGGGCTGCGACTCGGTCCGGTAAAGACTTAGTTGGGGCCGTGATTGGCGTCTACTCACTCGGGGTTGCGACTTCGATCTTCCTGAGTTTGTCTGGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

167

Amino Acids

17.28

Weight (kDa)

9.06

Isoelectric Point (pI)

38.03

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0017197)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G43540
fragaria_vesca FvH4_6g31340
malus_domestica MD04G1047100.v1.1
prunus_persica Prupe.1G201700_v2.0.a1
pyrus_communis pycom04g04090
rosa_chinensis RchiOBHm_Chr2g0138101
rosa_laevigata RLG00000019725
rosa_multiflora Rmu_sc0004576.1_g000001 Rmu_sc0019944.1_g000001
rosa_roxburghii Rroxscaffold_2G00106490
rosa_rugosa Rorug02G0349600
rosa_wichuraiana Rw2G032350 Rw2G032400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 237, 453
AciI CCGC 4 cut(s) 51, 185, 243, 267
AclWI GGATC 1 cut(s) 407
AcyI GRCGYC 3 cut(s) 120, 381, 450
AfiI CCNNNNNNNGG 1 cut(s) 205
AgsI TTSAA 1 cut(s) 301
AluBI AGCT 2 cut(s) 136, 319
AluI AGCT 2 cut(s) 136, 319
Alw21I GWGCWC 2 cut(s) 108, 321
AlwI GGATC 1 cut(s) 407
Ama87I CYCGRG 1 cut(s) 461
AoxI GGCC 8 cut(s) 12, 63, 75, 252, 287, 291, 354, 438
ApeKI GCWGC 1 cut(s) 407
AspLEI GCGC 1 cut(s) 332
AspS9I GGNCC 5 cut(s) 208, 287, 292, 417, 438
AsuC2I CCSGG 1 cut(s) 388
AsuHPI GGTGA 1 cut(s) 146
AvaI CYCGRG 1 cut(s) 461
AvaII GGWCC 2 cut(s) 208, 417
BanII GRGCYC 1 cut(s) 321
BbsI GAAGAC 1 cut(s) 115
Bbv12I GWGCWC 2 cut(s) 108, 321
BbvI GCAGC 1 cut(s) 394
BccI CCATC 1 cut(s) 220
BceAI ACGGC 4 cut(s) 133, 239, 341, 425
BcnI CCSGG 1 cut(s) 388
BfoI RGCGCY 1 cut(s) 333
BglI GCCNNNNNGGC 1 cut(s) 251
BisI GCNGC 1 cut(s) 408
BlsI GCNGC 1 cut(s) 409
Bme1390I CCNGG 1 cut(s) 388
Bme18I GGWCC 2 cut(s) 208, 417
BmeT110I CYCGRG 1 cut(s) 461
BmgT120I GGNCC 5 cut(s) 208, 287, 292, 417, 438
BmiI GGNNCC 3 cut(s) 209, 210, 439
BmrFI CCNGG 1 cut(s) 388
BmrI ACTGGG 2 cut(s) 214, 289
BmuI ACTGGG 2 cut(s) 214, 289
BpiI GAAGAC 1 cut(s) 115
BpuEI CTTGAG 1 cut(s) 233
BpuMI CCSGG 1 cut(s) 388
BsaHI GRCGYC 3 cut(s) 120, 381, 450
BsaWI WCCGGW 1 cut(s) 419
BsaXI ACNNNNNCTCC 2 cut(s) 77, 107
Bsc4I CCNNNNNNNGG 1 cut(s) 205
Bse118I RCCGGY 2 cut(s) 289, 326
Bse1I ACTGG 2 cut(s) 209, 295
BseGI GGATG 1 cut(s) 195
BseLI CCNNNNNNNGG 1 cut(s) 205
BseMII CTCAG 1 cut(s) 476
BseNI ACTGG 2 cut(s) 209, 295
BseXI GCAGC 1 cut(s) 394
BseYI CCCAGC 2 cut(s) 347, 395
Bsh1285I CGRYCG 1 cut(s) 378
BshFI GGCC 8 cut(s) 14, 65, 77, 254, 289, 293, 356, 440
BsiEI CGRYCG 1 cut(s) 378
BsiHKAI GWGCWC 2 cut(s) 108, 321
BsiHKCI CYCGRG 1 cut(s) 461
BsiSI CCGG 4 cut(s) 290, 327, 387, 420
BslFI GGGAC 2 cut(s) 194, 249
BslI CCNNNNNNNGG 1 cut(s) 205
BsmFI GGGAC 2 cut(s) 194, 249
BsmI GAATGC 1 cut(s) 349
BsnI GGCC 8 cut(s) 14, 65, 77, 254, 289, 293, 356, 440
BsoBI CYCGRG 1 cut(s) 461
Bsp1286I GDGCHC 2 cut(s) 108, 321
Bsp143I GATC 4 cut(s) 151, 375, 399, 477
BspACI CCGC 4 cut(s) 51, 185, 243, 267
BspANI GGCC 8 cut(s) 14, 65, 77, 254, 289, 293, 356, 440
BspCNI CTCAG 1 cut(s) 477
BspLI GGNNCC 3 cut(s) 209, 210, 439
BspPI GGATC 1 cut(s) 407
BspQI GCTCTTC 1 cut(s) 326
BsrFI RCCGGY 2 cut(s) 289, 326
BsrI ACTGG 2 cut(s) 209, 295
BssAI RCCGGY 2 cut(s) 289, 326
BssMI GATC 4 cut(s) 151, 375, 399, 477
BssNI GRCGYC 3 cut(s) 120, 381, 450
Bst6I CTCTTC 1 cut(s) 326
BstACI GRCGYC 3 cut(s) 120, 381, 450
BstC8I GCNNGC 5 cut(s) 51, 67, 108, 291, 328
BstDEI CTNAG 2 cut(s) 430, 485
BstF5I GGATG 1 cut(s) 195
BstH2I RGCGCY 1 cut(s) 333
BstHHI GCGC 1 cut(s) 332
BstKTI GATC 4 cut(s) 154, 378, 402, 480
BstMBI GATC 4 cut(s) 151, 375, 399, 477
BstMCI CGRYCG 1 cut(s) 378
BstMWI GCNNNNNNNGC 3 cut(s) 251, 353, 392
BstSCI CCNGG 1 cut(s) 386
BstV1I GCAGC 1 cut(s) 394
BstV2I GAAGAC 1 cut(s) 115
BstXI CCANNNNNNTGG 1 cut(s) 284
BsuRI GGCC 8 cut(s) 14, 65, 77, 254, 289, 293, 356, 440
BtsCI GGATG 1 cut(s) 195
BtsI GCAGTG 2 cut(s) 85, 311
BtsIMutI CAGTG 2 cut(s) 85, 311
Cac8I GCNNGC 5 cut(s) 51, 67, 108, 291, 328
CfoI GCGC 1 cut(s) 332
Cfr10I RCCGGY 2 cut(s) 289, 326
Cfr13I GGNCC 5 cut(s) 208, 287, 292, 417, 438
CpoI CGGWCCG 1 cut(s) 417
CseI GACGC 3 cut(s) 109, 370, 439
CspI CGGWCCG 1 cut(s) 417
DdeI CTNAG 2 cut(s) 430, 485
DpnI GATC 4 cut(s) 153, 377, 401, 479
DpnII GATC 4 cut(s) 151, 375, 399, 477
Eam1104I CTCTTC 1 cut(s) 326
EarI CTCTTC 1 cut(s) 326
EciI GGCGGA 1 cut(s) 232
Ecl136II GAGCTC 1 cut(s) 319
Eco24I GRGCYC 1 cut(s) 321
Eco47I GGWCC 2 cut(s) 208, 417
Eco53kI GAGCTC 1 cut(s) 319
Eco88I CYCGRG 1 cut(s) 461
EcoICRI GAGCTC 1 cut(s) 319
EcoO109I RGGNCCY 1 cut(s) 208
EcoT38I GRGCYC 1 cut(s) 321
FaqI GGGAC 2 cut(s) 194, 249
FauI CCCGC 3 cut(s) 58, 192, 274
FblI GTMKAC 2 cut(s) 237, 453
Fnu4HI GCNGC 1 cut(s) 408
FokI GGATG 1 cut(s) 182
FriOI GRGCYC 1 cut(s) 321
FseI GGCCGGCC 1 cut(s) 293
Fsp4HI GCNGC 1 cut(s) 408
GlaI GCGC 1 cut(s) 331
GluI GCNGC 1 cut(s) 408
GsaI CCCAGC 2 cut(s) 351, 399
HaeII RGCGCY 1 cut(s) 333
HaeIII GGCC 8 cut(s) 14, 65, 77, 254, 289, 293, 356, 440
HapII CCGG 4 cut(s) 290, 327, 387, 420
HgaI GACGC 3 cut(s) 109, 370, 439
HhaI GCGC 1 cut(s) 332
Hin1I GRCGYC 3 cut(s) 120, 381, 450
Hin6I GCGC 1 cut(s) 330
HinP1I GCGC 1 cut(s) 330
HincII GTYRAC 1 cut(s) 238
HindII GTYRAC 1 cut(s) 238
HinfI GANTC 7 cut(s) 6, 24, 59, 71, 223, 239, 412
HpaII CCGG 4 cut(s) 290, 327, 387, 420
HphI GGTGA 1 cut(s) 146
Hpy166II GTNNAC 2 cut(s) 238, 454
Hpy188I TCNGA 2 cut(s) 151, 375
Hpy188III TCNNGA 3 cut(s) 340, 484, 495
Hpy8I GTNNAC 2 cut(s) 238, 454
Hpy99I CGWCG 3 cut(s) 119, 341, 386
HpyAV CCTTC 3 cut(s) 64, 88, 346
HpyF10VI GCNNNNNNNGC 3 cut(s) 251, 353, 392
HpyF3I CTNAG 2 cut(s) 430, 485
Hsp92I GRCGYC 3 cut(s) 120, 381, 450
HspAI GCGC 1 cut(s) 330
KflI GGGWCCC 1 cut(s) 208
KroI GCCGGC 2 cut(s) 289, 326
KroNI GCCGGC 2 cut(s) 291, 328
Kzo9I GATC 4 cut(s) 151, 375, 399, 477
LguI GCTCTTC 1 cut(s) 326
LmnI GCTCC 2 cut(s) 141, 233
Lsp1109I GCAGC 1 cut(s) 394
MaeIII GTNAC 1 cut(s) 256
MalI GATC 4 cut(s) 153, 377, 401, 479
MboI GATC 4 cut(s) 151, 375, 399, 477
MboII GAAGA 3 cut(s) 115, 313, 472
MhlI GDGCHC 2 cut(s) 108, 321
MlyI GAGTC 6 cut(s) 33, 53, 65, 217, 233, 406
MmeI TCCRAC 1 cut(s) 66
MreI CGCCGGCG 1 cut(s) 326
MroNI GCCGGC 2 cut(s) 289, 326
MspI CCGG 4 cut(s) 290, 327, 387, 420
MspR9I CCNGG 1 cut(s) 388
Mva1269I GAATGC 1 cut(s) 349
MwoI GCNNNNNNNGC 3 cut(s) 251, 353, 392
NaeI GCCGGC 2 cut(s) 291, 328
NciI CCSGG 1 cut(s) 388
NdeII GATC 4 cut(s) 151, 375, 399, 477
NgoMIV GCCGGC 2 cut(s) 289, 326
NlaIV GGNNCC 3 cut(s) 209, 210, 439
NmeAIII GCCGAG 2 cut(s) 41, 53
NmuCI GTSAC 1 cut(s) 256
PciSI GCTCTTC 1 cut(s) 326
PctI GAATGC 1 cut(s) 349
PdiI GCCGGC 2 cut(s) 291, 328
PfeI GAWTC 1 cut(s) 6
PflFI GACNNNGTC 2 cut(s) 119, 415
PkrI GCNGC 1 cut(s) 409
Ple19I CGATCG 1 cut(s) 378
PleI GAGTC 6 cut(s) 32, 53, 65, 217, 233, 406
PpsI GAGTC 6 cut(s) 32, 53, 65, 217, 233, 406
PpuMI RGGWCCY 1 cut(s) 208
Psp124BI GAGCTC 1 cut(s) 321
Psp5II RGGWCCY 1 cut(s) 208
PspFI CCCAGC 2 cut(s) 347, 395
PspN4I GGNNCC 3 cut(s) 209, 210, 439
PspPI GGNCC 5 cut(s) 208, 287, 292, 417, 438
PspPPI RGGWCCY 1 cut(s) 208
PsyI GACNNNGTC 2 cut(s) 119, 415
PvuI CGATCG 1 cut(s) 378
RigI GGCCGGCC 1 cut(s) 293
Rsr2I CGGWCCG 1 cut(s) 417
RsrII CGGWCCG 1 cut(s) 417
SacI GAGCTC 1 cut(s) 321
SalI GTCGAC 1 cut(s) 236
SapI GCTCTTC 1 cut(s) 326
SatI GCNGC 1 cut(s) 408
Sau3AI GATC 4 cut(s) 151, 375, 399, 477
Sau96I GGNCC 5 cut(s) 208, 287, 292, 417, 438
SchI GAGTC 6 cut(s) 33, 53, 65, 217, 233, 406
ScrFI CCNGG 1 cut(s) 388
SduI GDGCHC 2 cut(s) 108, 321
SetI ASST 3 cut(s) 138, 159, 321
SgrAI CRCCGGYG 1 cut(s) 326
SinI GGWCC 2 cut(s) 208, 417
SmlI CTYRAG 1 cut(s) 248
SmoI CTYRAG 1 cut(s) 248
SsiI CCGC 4 cut(s) 51, 185, 243, 267
SstI GAGCTC 1 cut(s) 321
StyD4I CCNGG 1 cut(s) 386
TaqI TCGA 6 cut(s) 57, 114, 237, 339, 367, 476
TaqII GACCGA 1 cut(s) 405
TfiI GAWTC 1 cut(s) 6
TscAI CASTG 2 cut(s) 92, 311
TseFI GTSAC 1 cut(s) 256
TseI GCWGC 1 cut(s) 407
Tsp45I GTSAC 1 cut(s) 256
TspRI CASTG 2 cut(s) 92, 311
Tth111I GACNNNGTC 2 cut(s) 119, 415
VpaK11BI GGWCC 2 cut(s) 208, 417
XmiI GTMKAC 2 cut(s) 237, 453
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.