MD04G1049100.v1.1

15 kDa selenoprotein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0014141
Physical Location & Seq
Reverse (-)
5752499 .. 5757177
4679 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1049100.v1.1.491

Sequence Viewer

Length: 600 bp
ATGGTGCCTAAAGCCCGAAACGAATCGAAGGCCCAGACCAAGAGCAAGACCAGCGTTTTGTTTTTGCATCGAGTCTTGGGAGGAGAGACTGAAACTCGTGAGCTCGAAGGTAGAGAGATGGGAGCGTTGGTGGCGGCTATGTTGGTGGTGCTCGCGGTGGCAGTAGCTTCCGGTGCATCAACATCGAAAGAGCAGCTGAGCACAAGGGAATGCGAGAATCTTGGCTTCACAGGTCTCGCTTTGTGCTCTGATTGTAACAGTTTGGCTGAGTATGTTAAGGATCAAGAATTGGTATCCAACTGTATGAAATGTTGCACCGAGGATTCCGATGATTCCACAACAAAGATCACGTATTCTGGTGCCATACTGGAAGTCTGCATGAGGAAACTGGTTTTCTATCCGGAAATTGTTGGCTTTATTGAAGAAGAGAAGGATCGGTTCCCTTCGGTCAAAGTTCAATACATTTTCAATTCTCCACCAAAGTTGATCCTGCTAGATGATGCAGGCGAACGTAAGGAAACAATAAGAATCGACAATTGGAAGCGTGAACATATCTTGCAGTTCCTGCAAGGGAAGGTAAAGCCTGCTTCTGCAATCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Sep15_SelM

Protein Analysis

200

Amino Acids

22.09

Weight (kDa)

5.59

Isoelectric Point (pI)

43.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Sep15_SelM PF08806 120 - 193 1.5e-28 Sep15/SelM redox domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014141)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G05720
fragaria_vesca FvH4_4g10450 FvH4_4g10450
malus_domestica MD04G1049100.v1.1 MD17G1177800.v1.1
prunus_persica Prupe.1G207200_v2.0.a1
pyrus_communis pycom04g04330
rosa_chinensis RchiOBHm_Chr4g0405941
rosa_laevigata RLG00000008809
rosa_multiflora Rmu_ssc0000087.1_g000072
rosa_roxburghii Rroxscaffold_5G00350680
rosa_rugosa Rorug04G0064200
rosa_samantha Rh4AG135000 Rh4BG130600 Rh4CG142400 Rh4DG129300
rosa_wichuraiana Rw4G010990

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 4, 359
AccII CGCG 1 cut(s) 155
AccIII TCCGGA 1 cut(s) 400
AciI CCGC 2 cut(s) 134, 155
AclWI GGATC 3 cut(s) 288, 441, 481
AgsI TTSAA 3 cut(s) 422, 458, 469
AjuI GAANNNNNNNTTGG 2 cut(s) 520, 552
AluBI AGCT 3 cut(s) 103, 167, 196
AluI AGCT 3 cut(s) 103, 167, 196
Alw21I GWGCWC 4 cut(s) 105, 153, 203, 248
Alw26I GTCTC 2 cut(s) 80, 239
AlwI GGATC 3 cut(s) 288, 441, 481
AlwNI CAGNNNCTG 1 cut(s) 565
Aor13HI TCCGGA 1 cut(s) 400
AoxI GGCC 1 cut(s) 30
ApeKI GCWGC 1 cut(s) 193
ArsI GACNNNNNNTTYG 2 cut(s) 40, 72
AspS9I GGNCC 1 cut(s) 31
BanI GGYRCC 2 cut(s) 4, 359
BanII GRGCYC 1 cut(s) 105
BauI CACGAG 1 cut(s) 96
Bbv12I GWGCWC 4 cut(s) 105, 153, 203, 248
BbvI GCAGC 1 cut(s) 205
BccI CCATC 1 cut(s) 112
BcgI CGANNNNNNTGC 2 cut(s) 165, 199
BciVI GTATCC 1 cut(s) 304
BcoDI GTCTC 2 cut(s) 80, 239
BfaI CTAG 1 cut(s) 494
BfuI GTATCC 1 cut(s) 304
BisI GCNGC 2 cut(s) 135, 194
BlpI GCTNAGC 1 cut(s) 197
BlsI GCNGC 2 cut(s) 136, 195
BmgT120I GGNCC 1 cut(s) 31
BmiI GGNNCC 3 cut(s) 6, 361, 440
BmsI GCATC 3 cut(s) 76, 185, 490
Bpu1102I GCTNAGC 1 cut(s) 197
BsaAI YACGTR 1 cut(s) 351
BsaI GGTCTC 1 cut(s) 239
BsaJI CCNNGG 1 cut(s) 318
BsaWI WCCGGW 2 cut(s) 170, 400
BsaXI ACNNNNNCTCC 2 cut(s) 114, 144
Bse1I ACTGG 2 cut(s) 372, 393
BseAI TCCGGA 1 cut(s) 400
BseDI CCNNGG 1 cut(s) 318
BseMII CTCAG 2 cut(s) 188, 258
BseNI ACTGG 2 cut(s) 372, 393
BseRI GAGGAG 1 cut(s) 96
BseXI GCAGC 1 cut(s) 205
Bsh1236I CGCG 1 cut(s) 155
BshFI GGCC 1 cut(s) 32
BshNI GGYRCC 2 cut(s) 4, 359
BsiHKAI GWGCWC 4 cut(s) 105, 153, 203, 248
BsiSI CCGG 2 cut(s) 171, 401
BsmAI GTCTC 2 cut(s) 80, 239
BsmI GAATGC 1 cut(s) 215
BsnI GGCC 1 cut(s) 32
Bso31I GGTCTC 1 cut(s) 239
Bsp1286I GDGCHC 4 cut(s) 105, 153, 203, 248
Bsp13I TCCGGA 1 cut(s) 400
Bsp143I GATC 4 cut(s) 280, 345, 433, 486
Bsp1720I GCTNAGC 1 cut(s) 197
BspACI CCGC 2 cut(s) 134, 155
BspANI GGCC 1 cut(s) 32
BspCNI CTCAG 2 cut(s) 189, 259
BspEI TCCGGA 1 cut(s) 400
BspFNI CGCG 1 cut(s) 155
BspLI GGNNCC 3 cut(s) 6, 361, 440
BspPI GGATC 3 cut(s) 288, 441, 481
BspT107I GGYRCC 2 cut(s) 4, 359
BspTNI GGTCTC 1 cut(s) 239
BsrI ACTGG 2 cut(s) 372, 393
BssECI CCNNGG 1 cut(s) 318
BssMI GATC 4 cut(s) 280, 345, 433, 486
BssSI CACGAG 1 cut(s) 96
Bst2BI CACGAG 1 cut(s) 96
Bst4CI ACNGT 2 cut(s) 260, 302
Bst6I CTCTTC 1 cut(s) 420
BstAPI GCANNNNNTGC 1 cut(s) 565
BstBAI YACGTR 1 cut(s) 351
BstC8I GCNNGC 3 cut(s) 153, 505, 585
BstDEI CTNAG 2 cut(s) 197, 267
BstFNI CGCG 1 cut(s) 155
BstKTI GATC 4 cut(s) 283, 348, 436, 489
BstMAI GTCTC 2 cut(s) 80, 239
BstMBI GATC 4 cut(s) 280, 345, 433, 486
BstMWI GCNNNNNNNGC 4 cut(s) 51, 131, 173, 565
BstUI CGCG 1 cut(s) 155
BstV1I GCAGC 1 cut(s) 205
BsuI GTATCC 1 cut(s) 304
BsuRI GGCC 1 cut(s) 32
Cac8I GCNNGC 3 cut(s) 153, 505, 585
CaiI CAGNNNCTG 1 cut(s) 565
Cfr13I GGNCC 1 cut(s) 31
CspCI CAANNNNNGTGG 2 cut(s) 465, 500
CviAII CATG 1 cut(s) 379
DdeI CTNAG 2 cut(s) 197, 267
DpnI GATC 4 cut(s) 282, 347, 435, 488
DpnII GATC 4 cut(s) 280, 345, 433, 486
Eam1104I CTCTTC 1 cut(s) 420
EarI CTCTTC 1 cut(s) 420
Ecl136II GAGCTC 1 cut(s) 103
Eco24I GRGCYC 1 cut(s) 105
Eco31I GGTCTC 1 cut(s) 239
Eco53kI GAGCTC 1 cut(s) 103
EcoICRI GAGCTC 1 cut(s) 103
EcoT38I GRGCYC 1 cut(s) 105
FaeI CATG 1 cut(s) 382
FaiI YATR 6 cut(s) 140, 273, 305, 365, 380, 552
FatI CATG 1 cut(s) 378
Fnu4HI GCNGC 2 cut(s) 135, 194
FriOI GRGCYC 1 cut(s) 105
Fsp4HI GCNGC 2 cut(s) 135, 194
FspBI CTAG 1 cut(s) 494
GluI GCNGC 2 cut(s) 135, 194
HaeIII GGCC 1 cut(s) 32
HapII CCGG 2 cut(s) 171, 401
Hin1II CATG 1 cut(s) 382
HinfI GANTC 6 cut(s) 23, 72, 217, 323, 332, 528
HpaII CCGG 2 cut(s) 171, 401
Hpy166II GTNNAC 1 cut(s) 548
Hpy188I TCNGA 3 cut(s) 250, 328, 599
Hpy188III TCNNGA 3 cut(s) 98, 284, 401
Hpy8I GTNNAC 1 cut(s) 548
HpyAV CCTTC 5 cut(s) 22, 101, 424, 453, 568
HpyCH4III ACNGT 2 cut(s) 260, 302
HpyCH4IV ACGT 2 cut(s) 350, 511
HpyCH4V TGCA 8 cut(s) 67, 176, 315, 378, 503, 559, 568, 593
HpyF10VI GCNNNNNNNGC 4 cut(s) 51, 131, 173, 565
HpyF3I CTNAG 2 cut(s) 197, 267
HpySE526I ACGT 2 cut(s) 350, 511
Hsp92II CATG 1 cut(s) 382
Kpn2I TCCGGA 1 cut(s) 400
Kzo9I GATC 4 cut(s) 280, 345, 433, 486
LmnI GCTCC 1 cut(s) 122
Lsp1109I GCAGC 1 cut(s) 205
LweI GCATC 3 cut(s) 76, 185, 490
MaeI CTAG 1 cut(s) 494
MaeII ACGT 2 cut(s) 350, 511
MaeIII GTNAC 1 cut(s) 254
MalI GATC 4 cut(s) 282, 347, 435, 488
MboI GATC 4 cut(s) 280, 345, 433, 486
MboII GAAGA 2 cut(s) 434, 437
MfeI CAATTG 1 cut(s) 535
MhlI GDGCHC 4 cut(s) 105, 153, 203, 248
MluCI AATT 4 cut(s) 287, 405, 469, 535
MlyI GAGTC 1 cut(s) 81
MmeI TCCRAC 1 cut(s) 321
MnlI CCTC 3 cut(s) 74, 313, 375
MroI TCCGGA 1 cut(s) 400
MseI TTAA 1 cut(s) 276
MspA1I CMGCKG 1 cut(s) 196
MspI CCGG 2 cut(s) 171, 401
MunI CAATTG 1 cut(s) 535
Mva1269I GAATGC 1 cut(s) 215
MvnI CGCG 1 cut(s) 155
MwoI GCNNNNNNNGC 4 cut(s) 51, 131, 173, 565
NdeII GATC 4 cut(s) 280, 345, 433, 486
NlaIII CATG 1 cut(s) 382
NlaIV GGNNCC 3 cut(s) 6, 361, 440
PctI GAATGC 1 cut(s) 215
PfeI GAWTC 5 cut(s) 23, 217, 323, 332, 528
PkrI GCNGC 2 cut(s) 136, 195
PleI GAGTC 1 cut(s) 80
PpsI GAGTC 1 cut(s) 80
Ppu21I YACGTR 1 cut(s) 351
Psp124BI GAGCTC 1 cut(s) 105
PspN4I GGNNCC 3 cut(s) 6, 361, 440
PspPI GGNCC 1 cut(s) 31
PstNI CAGNNNCTG 1 cut(s) 565
PvuII CAGCTG 1 cut(s) 196
SacI GAGCTC 1 cut(s) 105
SaqAI TTAA 1 cut(s) 276
SatI GCNGC 2 cut(s) 135, 194
Sau3AI GATC 4 cut(s) 280, 345, 433, 486
Sau96I GGNCC 1 cut(s) 31
SchI GAGTC 1 cut(s) 81
SduI GDGCHC 4 cut(s) 105, 153, 203, 248
SetI ASST 8 cut(s) 105, 112, 169, 198, 235, 353, 514, 579
SfaNI GCATC 3 cut(s) 76, 185, 490
Sse9I AATT 4 cut(s) 287, 405, 469, 535
SsiI CCGC 2 cut(s) 134, 155
SspMI CTAG 1 cut(s) 494
SstI GAGCTC 1 cut(s) 105
TaaI ACNGT 2 cut(s) 260, 302
TaiI ACGT 2 cut(s) 353, 514
TaqI TCGA 5 cut(s) 26, 70, 105, 185, 531
TaqII GACCGA 1 cut(s) 436
TasI AATT 4 cut(s) 287, 405, 469, 535
TauI GCSGC 1 cut(s) 137
TfiI GAWTC 5 cut(s) 23, 217, 323, 332, 528
Tru1I TTAA 1 cut(s) 276
Tru9I TTAA 1 cut(s) 276
TseI GCWGC 1 cut(s) 193
TspDTI ATGAA 1 cut(s) 320
XspI CTAG 1 cut(s) 494
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.