MD04G1049300.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0008474
Physical Location & Seq
Forward (+)
5769740 .. 5773199
3460 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1049300.v1.1.491

Sequence Viewer

Length: 768 bp
ATGCAAGATCCAAGTAACTCACAAGCAAGGAATAAGCCTTGGTATCAAAGAGCAATGGAGATGGGCAGTTTGTGGAGAAGCATCAGCAATACTTCCAAGTCCACACAAGTTCCAGCATCAACTTCATCCACGCTATGGAAAACCATTGTTAATGTTAGGAGTGGGCACTTGGAACCGAAAACCGAAACCGAAACACGAAACAAACCAAGCACGCCTAATGTCCAAAGTGGTGGCAGTAGTACTGCTACTGGAACAAACAATCACACTTTTTCTGCAGCTTCCAGTTCGAGTTCGAGTTCGAGTTCCAGTAACAATAATATTAGACATGCCAACAAACTGCGAAAATGTACCTCTCTAAAGGTTGCCACTTCATTTACAAGGGTGTGCCTTTGTGCACCAATATCTTCCTACAATGAGGTTTTCAGAGCCGAGCTTCCCCCAAGAAGAAGCAATAGTTACCCGAGAACGCGATCGTATTCCAAGCCACTTGGTACAACTAGTAGCAGTACTACTGCACAAGCACCACAACAAGCAATGAGCGCAAGACGACTTAGTACCGACCAAGGAATCAGAAGAGTGTTTCGAGGAAAGTCATTGACCGACGACGTGTTGATGAGAAGGTTTGTTGTAGAAGAAGAGGCAATGATGCAAGTTAGAAGGAGAAATCAAATGGAAGTCATAAGGAGGAGAAGTGTGATGAGGAGGAAGAGGCTTGGCCCCAGTCCTCTTAGTAGAATGGTTATGGCTCAGGTTCAGGAGGAGGATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

256

Amino Acids

28.54

Weight (kDa)

11.36

Isoelectric Point (pI)

78.39

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 135
AccII CGCG 1 cut(s) 469
AclWI GGATC 1 cut(s) 2
AfaI GTAC 5 cut(s) 241, 349, 493, 508, 556
AfiI CCNNNNNNNGG 1 cut(s) 135
AflIII ACRYGT 1 cut(s) 606
AhlI ACTAGT 1 cut(s) 497
AjiI CACGTC 1 cut(s) 607
AluBI AGCT 2 cut(s) 278, 433
AluI AGCT 2 cut(s) 278, 433
Alw21I GWGCWC 1 cut(s) 397
Alw44I GTGCAC 1 cut(s) 393
AlwI GGATC 1 cut(s) 2
Ama87I CYCGRG 1 cut(s) 460
AoxI GGCC 1 cut(s) 715
ApaLI GTGCAC 1 cut(s) 393
ApeKI GCWGC 1 cut(s) 275
AspLEI GCGC 1 cut(s) 542
AspS9I GGNCC 1 cut(s) 716
AvaI CYCGRG 1 cut(s) 460
BaeGI GKGCMC 2 cut(s) 168, 397
Bbv12I GWGCWC 1 cut(s) 397
BbvI GCAGC 1 cut(s) 287
BccI CCATC 1 cut(s) 55
BcuI ACTAGT 1 cut(s) 497
BfaI CTAG 1 cut(s) 498
BfmI CTRYAG 1 cut(s) 273
BisI GCNGC 1 cut(s) 276
BlsI GCNGC 1 cut(s) 277
BmcAI AGTACT 2 cut(s) 241, 508
BmeT110I CYCGRG 1 cut(s) 460
BmgBI CACGTC 1 cut(s) 607
BmgT120I GGNCC 1 cut(s) 716
BmiI GGNNCC 2 cut(s) 174, 718
BmrI ACTGGG 1 cut(s) 714
BmsI GCATC 3 cut(s) 90, 125, 636
BmuI ACTGGG 1 cut(s) 714
Bpu10I CCTNAGC 1 cut(s) 747
BsaJI CCNNGG 2 cut(s) 38, 562
BsaXI ACNNNNNCTCC 2 cut(s) 676, 706
Bsc4I CCNNNNNNNGG 1 cut(s) 135
Bse1I ACTGG 4 cut(s) 253, 282, 306, 720
Bse3DI GCAATG 3 cut(s) 60, 540, 648
BseDI CCNNGG 2 cut(s) 38, 562
BseGI GGATG 1 cut(s) 125
BseLI CCNNNNNNNGG 1 cut(s) 135
BseMI GCAATG 3 cut(s) 60, 540, 648
BseMII CTCAG 1 cut(s) 761
BseNI ACTGG 4 cut(s) 253, 282, 306, 720
BseRI GAGGAG 2 cut(s) 700, 715
BseSI GKGCMC 2 cut(s) 168, 397
BseXI GCAGC 1 cut(s) 287
BsgI GTGCAG 1 cut(s) 498
Bsh1236I CGCG 1 cut(s) 469
Bsh1285I CGRYCG 1 cut(s) 473
BshFI GGCC 1 cut(s) 717
BsiEI CGRYCG 1 cut(s) 473
BsiHKAI GWGCWC 1 cut(s) 397
BsiHKCI CYCGRG 1 cut(s) 460
BslI CCNNNNNNNGG 1 cut(s) 135
BsnI GGCC 1 cut(s) 717
BsoBI CYCGRG 1 cut(s) 460
Bsp1286I GDGCHC 2 cut(s) 168, 397
Bsp143I GATC 2 cut(s) 7, 470
BspANI GGCC 1 cut(s) 717
BspCNI CTCAG 1 cut(s) 760
BspFNI CGCG 1 cut(s) 469
BspLI GGNNCC 2 cut(s) 174, 718
BspMAI CTGCAG 1 cut(s) 277
BspPI GGATC 1 cut(s) 2
BsrDI GCAATG 3 cut(s) 60, 540, 648
BsrI ACTGG 4 cut(s) 253, 282, 306, 720
BssECI CCNNGG 2 cut(s) 38, 562
BssMI GATC 2 cut(s) 7, 470
BssT1I CCWWGG 2 cut(s) 38, 562
Bst6I CTCTTC 3 cut(s) 568, 630, 701
BstC8I GCNNGC 1 cut(s) 212
BstDEI CTNAG 3 cut(s) 551, 728, 747
BstF5I GGATG 1 cut(s) 125
BstFNI CGCG 1 cut(s) 469
BstHHI GCGC 1 cut(s) 542
BstKTI GATC 2 cut(s) 10, 473
BstMBI GATC 2 cut(s) 7, 470
BstMCI CGRYCG 1 cut(s) 473
BstMWI GCNNNNNNNGC 1 cut(s) 539
BstNSI RCATGY 1 cut(s) 329
BstSFI CTRYAG 1 cut(s) 273
BstSLI GKGCMC 2 cut(s) 168, 397
BstUI CGCG 1 cut(s) 469
BstV1I GCAGC 1 cut(s) 287
BstX2I RGATCY 1 cut(s) 7
BstXI CCANNNNNNTGG 1 cut(s) 230
BstYI RGATCY 1 cut(s) 7
BsuRI GGCC 1 cut(s) 717
BtrI CACGTC 1 cut(s) 607
BtsCI GGATG 1 cut(s) 125
Cac8I GCNNGC 1 cut(s) 212
CfoI GCGC 1 cut(s) 542
Cfr13I GGNCC 1 cut(s) 716
Csp6I GTAC 5 cut(s) 240, 348, 492, 507, 555
CviAII CATG 1 cut(s) 326
CviJI RGCY 8 cut(s) 37, 278, 428, 433, 484, 712, 717, 746
CviKI_1 RGCY 8 cut(s) 37, 278, 428, 433, 484, 712, 717, 746
CviQI GTAC 5 cut(s) 240, 348, 492, 507, 555
DdeI CTNAG 3 cut(s) 551, 728, 747
DpnI GATC 2 cut(s) 9, 472
DpnII GATC 2 cut(s) 7, 470
Eam1104I CTCTTC 3 cut(s) 568, 630, 701
EarI CTCTTC 3 cut(s) 568, 630, 701
Eco130I CCWWGG 2 cut(s) 38, 562
Eco88I CYCGRG 1 cut(s) 460
EcoT14I CCWWGG 2 cut(s) 38, 562
ErhI CCWWGG 2 cut(s) 38, 562
FaeI CATG 1 cut(s) 329
FaiI YATR 4 cut(s) 136, 327, 680, 743
FatI CATG 1 cut(s) 325
Fnu4HI GCNGC 1 cut(s) 276
FokI GGATG 1 cut(s) 112
Fsp4HI GCNGC 1 cut(s) 276
FspBI CTAG 1 cut(s) 498
GlaI GCGC 1 cut(s) 541
GluI GCNGC 1 cut(s) 276
HaeIII GGCC 1 cut(s) 717
HhaI GCGC 1 cut(s) 542
Hin1II CATG 1 cut(s) 329
Hin6I GCGC 1 cut(s) 540
HinP1I GCGC 1 cut(s) 540
HinfI GANTC 1 cut(s) 567
Hpy166II GTNNAC 2 cut(s) 102, 395
Hpy188I TCNGA 2 cut(s) 425, 572
Hpy188III TCNNGA 1 cut(s) 755
Hpy8I GTNNAC 2 cut(s) 102, 395
Hpy99I CGWCG 2 cut(s) 605, 608
HpyAV CCTTC 2 cut(s) 612, 651
HpyCH4IV ACGT 1 cut(s) 606
HpyCH4V TGCA 5 cut(s) 4, 275, 395, 515, 649
HpyF10VI GCNNNNNNNGC 1 cut(s) 539
HpyF3I CTNAG 3 cut(s) 551, 728, 747
HpySE526I ACGT 1 cut(s) 606
Hsp92II CATG 1 cut(s) 329
HspAI GCGC 1 cut(s) 540
Kzo9I GATC 2 cut(s) 7, 470
LpnPI CCDG 7 cut(s) 126, 234, 295, 319, 733, 734, 740
Lsp1109I GCAGC 1 cut(s) 287
LweI GCATC 3 cut(s) 90, 125, 636
MaeI CTAG 1 cut(s) 498
MaeII ACGT 1 cut(s) 606
MaeIII GTNAC 3 cut(s) 14, 308, 455
MalI GATC 2 cut(s) 9, 472
MboI GATC 2 cut(s) 7, 470
MboII GAAGA 6 cut(s) 396, 456, 585, 644, 647, 718
MflI RGATCY 1 cut(s) 7
MhlI GDGCHC 2 cut(s) 168, 397
MseI TTAA 2 cut(s) 150, 766
MvnI CGCG 1 cut(s) 469
MwoI GCNNNNNNNGC 1 cut(s) 539
NdeII GATC 2 cut(s) 7, 470
NlaIII CATG 1 cut(s) 329
NlaIV GGNNCC 2 cut(s) 174, 718
NmeAIII GCCGAG 1 cut(s) 454
NspI RCATGY 1 cut(s) 329
PfeI GAWTC 1 cut(s) 567
PflMI CCANNNNNTGG 1 cut(s) 135
PkrI GCNGC 1 cut(s) 277
Ple19I CGATCG 1 cut(s) 473
PspN4I GGNNCC 2 cut(s) 174, 718
PspPI GGNCC 1 cut(s) 716
PstI CTGCAG 1 cut(s) 277
PsuI RGATCY 1 cut(s) 7
PvuI CGATCG 1 cut(s) 473
RsaI GTAC 5 cut(s) 241, 349, 493, 508, 556
RsaNI GTAC 5 cut(s) 240, 348, 492, 507, 555
SaqAI TTAA 2 cut(s) 150, 766
SatI GCNGC 1 cut(s) 276
Sau3AI GATC 2 cut(s) 7, 470
Sau96I GGNCC 1 cut(s) 716
ScaI AGTACT 2 cut(s) 241, 508
SduI GDGCHC 2 cut(s) 168, 397
SetI ASST 8 cut(s) 280, 353, 363, 420, 435, 609, 623, 753
SfaNI GCATC 3 cut(s) 90, 125, 636
SfcI CTRYAG 1 cut(s) 273
SpeI ACTAGT 1 cut(s) 497
SspI AATATT 1 cut(s) 319
SspMI CTAG 1 cut(s) 498
StyI CCWWGG 2 cut(s) 38, 562
TaiI ACGT 1 cut(s) 609
TaqI TCGA 4 cut(s) 287, 293, 299, 583
TaqII GACCGA 1 cut(s) 614
TatI WGTACW 2 cut(s) 239, 506
TfiI GAWTC 1 cut(s) 567
Tru1I TTAA 2 cut(s) 150, 766
Tru9I TTAA 2 cut(s) 150, 766
TseI GCWGC 1 cut(s) 275
TspDTI ATGAA 2 cut(s) 114, 360
Van91I CCANNNNNTGG 1 cut(s) 135
VneI GTGCAC 1 cut(s) 393
XceI RCATGY 1 cut(s) 329
XspI CTAG 1 cut(s) 498
ZrmI AGTACT 2 cut(s) 241, 508
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.