Rorug04G0063800

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0008474
Physical Location & Seq
Reverse (-)
9905814 .. 9906554
741 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0063800.1

Sequence Viewer

Length: 741 bp
ATGGCTGATATTCCTGATAAAGAACTCCATTGTTGCGCAAAAAGGACGAAGGTTACAGGCAGCGGGAACTACCTAAAATCCTGTAGTACAAGTAGTAGCACTACCAGCAGCAGTATCAGTTTAGCAGAAATTATAGCCAGCACGGAGGAACTCCTTATGGAAATCCTACTGCGCGTGCCAGCTCGAACTCTGGTCCGCTTCAAATGTGTTTCCAAGCATTGGCTCGCTCTTATATCTGACCCCAAATTCTGTCACCGCCACACCCTTCGGAACCAAAACTCTTCCATCTCCGCAGTCTTTGGTGACAGATCCGTAGACCTTTTTTCCATCATTTTTTCTCTTGATCATGATCGTAGGGGTAGTAACCATGGAAACCATAACCCCGGATCATCCCGCTGCAATCCTCTTAATTTTGTTCAAAACCAACATGATCCCATCGAGATTATCCACTCCTGCAATGGCCTCTTCTTGTGTCGTCCCATCAATGCTACAAGTACTAGTAGAACCCCATATTTTGTTCTTAATCCCACAACCAATCAGTTCTTGACGCTTACAATTCCCCCAGCTGCTGCTGCTACTTCTAATGGTCAACAGATACAAACCCATATTATTGGTTGTGCTGTGGCTTTTGACCCTTCCAAATCACCTCATTACAAGGTTATATGCCTTGAGACCACTACTTGTAATACCCCGAAAATCCAAATTAAATTCCATGGATTTTTAGAAATGATTTTACGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

246

Amino Acids

27.22

Weight (kDa)

8.91

Isoelectric Point (pI)

39.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box PF00646 49 - 85 5.6e-08 F-box domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 37
AccB7I CCANNNNNTGG 1 cut(s) 219
AccI GTMKAC 1 cut(s) 315
AccII CGCG 1 cut(s) 174
AciI CCGC 5 cut(s) 63, 196, 256, 291, 394
AclWI GGATC 3 cut(s) 303, 394, 425
AcsI RAATTY 2 cut(s) 245, 707
AfaI GTAC 2 cut(s) 88, 496
AfiI CCNNNNNNNGG 1 cut(s) 219
AgsI TTSAA 2 cut(s) 202, 419
AhlI ACTAGT 1 cut(s) 497
AluBI AGCT 2 cut(s) 182, 566
AluI AGCT 2 cut(s) 182, 566
Alw26I GTCTC 1 cut(s) 665
AlwI GGATC 3 cut(s) 303, 394, 425
AlwNI CAGNNNCTG 1 cut(s) 569
AoxI GGCC 1 cut(s) 460
ApeKI GCWGC 6 cut(s) 60, 108, 396, 566, 569, 572
ApoI RAATTY 2 cut(s) 245, 707
AspLEI GCGC 2 cut(s) 38, 174
AspS9I GGNCC 1 cut(s) 193
AsuC2I CCSGG 1 cut(s) 384
AsuHPI GGTGA 3 cut(s) 245, 314, 636
AvaII GGWCC 1 cut(s) 193
BbvI GCAGC 6 cut(s) 72, 120, 383, 553, 556, 559
BccI CCATC 4 cut(s) 293, 335, 443, 488
BclI TGATCA 1 cut(s) 343
BcnI CCSGG 1 cut(s) 384
BcoDI GTCTC 1 cut(s) 665
BcuI ACTAGT 1 cut(s) 497
BfaI CTAG 1 cut(s) 498
BfmI CTRYAG 1 cut(s) 82
BisI GCNGC 6 cut(s) 61, 109, 397, 567, 570, 573
BlsI GCNGC 6 cut(s) 62, 110, 398, 568, 571, 574
BmcAI AGTACT 1 cut(s) 496
Bme1390I CCNGG 1 cut(s) 384
Bme18I GGWCC 1 cut(s) 193
BmgT120I GGNCC 1 cut(s) 193
BmiI GGNNCC 1 cut(s) 272
BmrFI CCNGG 1 cut(s) 384
BpuEI CTTGAG 1 cut(s) 689
BpuMI CCSGG 1 cut(s) 384
BsaBI GATNNNNATC 1 cut(s) 348
BsaI GGTCTC 1 cut(s) 665
BsaJI CCNNGG 3 cut(s) 367, 382, 712
Bsc4I CCNNNNNNNGG 1 cut(s) 219
Bse3DI GCAATG 1 cut(s) 463
Bse8I GATNNNNATC 1 cut(s) 348
BseDI CCNNGG 3 cut(s) 367, 382, 712
BseGI GGATG 1 cut(s) 389
BseJI GATNNNNATC 1 cut(s) 348
BseLI CCNNNNNNNGG 1 cut(s) 219
BseMI GCAATG 1 cut(s) 463
BseXI GCAGC 6 cut(s) 72, 120, 383, 553, 556, 559
BseYI CCCAGC 1 cut(s) 562
Bsh1236I CGCG 1 cut(s) 174
BshFI GGCC 1 cut(s) 462
BsiSI CCGG 1 cut(s) 384
BslFI GGGAC 1 cut(s) 462
BslI CCNNNNNNNGG 1 cut(s) 219
BsmAI GTCTC 1 cut(s) 665
BsmFI GGGAC 1 cut(s) 462
BsnI GGCC 1 cut(s) 462
Bso31I GGTCTC 1 cut(s) 665
Bsp143I GATC 5 cut(s) 308, 343, 349, 386, 430
Bsp19I CCATGG 2 cut(s) 367, 712
BspACI CCGC 5 cut(s) 63, 196, 256, 291, 394
BspANI GGCC 1 cut(s) 462
BspFNI CGCG 1 cut(s) 174
BspHI TCATGA 1 cut(s) 346
BspLI GGNNCC 1 cut(s) 272
BspPI GGATC 3 cut(s) 303, 394, 425
BspTNI GGTCTC 1 cut(s) 665
BsrDI GCAATG 1 cut(s) 463
BssECI CCNNGG 3 cut(s) 367, 382, 712
BssMI GATC 5 cut(s) 308, 343, 349, 386, 430
BssT1I CCWWGG 2 cut(s) 367, 712
Bst6I CTCTTC 2 cut(s) 286, 470
BstC8I GCNNGC 4 cut(s) 139, 176, 180, 225
BstDSI CCRYGG 2 cut(s) 367, 712
BstF5I GGATG 1 cut(s) 389
BstFNI CGCG 1 cut(s) 174
BstHHI GCGC 2 cut(s) 38, 174
BstKTI GATC 5 cut(s) 311, 346, 352, 389, 433
BstMAI GTCTC 1 cut(s) 665
BstMBI GATC 5 cut(s) 308, 343, 349, 386, 430
BstMWI GCNNNNNNNGC 2 cut(s) 105, 572
BstSCI CCNGG 1 cut(s) 382
BstSFI CTRYAG 1 cut(s) 82
BstUI CGCG 1 cut(s) 174
BstV1I GCAGC 6 cut(s) 72, 120, 383, 553, 556, 559
BstX2I RGATCY 1 cut(s) 308
BstXI CCANNNNNNTGG 1 cut(s) 611
BstYI RGATCY 1 cut(s) 308
BsuRI GGCC 1 cut(s) 462
BtgI CCRYGG 2 cut(s) 367, 712
BtsCI GGATG 1 cut(s) 389
Cac8I GCNNGC 4 cut(s) 139, 176, 180, 225
CaiI CAGNNNCTG 1 cut(s) 569
CciI TCATGA 1 cut(s) 346
CfoI GCGC 2 cut(s) 38, 174
Cfr13I GGNCC 1 cut(s) 193
CseI GACGC 1 cut(s) 556
Csp6I GTAC 2 cut(s) 87, 495
CviAII CATG 4 cut(s) 347, 368, 428, 713
CviJI RGCY 7 cut(s) 5, 137, 182, 223, 462, 566, 626
CviKI_1 RGCY 7 cut(s) 5, 137, 182, 223, 462, 566, 626
CviQI GTAC 2 cut(s) 87, 495
DpnI GATC 5 cut(s) 310, 345, 351, 388, 432
DpnII GATC 5 cut(s) 308, 343, 349, 386, 430
Eam1104I CTCTTC 2 cut(s) 286, 470
EarI CTCTTC 2 cut(s) 286, 470
Eco130I CCWWGG 2 cut(s) 367, 712
Eco31I GGTCTC 1 cut(s) 665
Eco47I GGWCC 1 cut(s) 193
EcoT14I CCWWGG 2 cut(s) 367, 712
ErhI CCWWGG 2 cut(s) 367, 712
FaeI CATG 4 cut(s) 350, 371, 431, 716
FaqI GGGAC 1 cut(s) 462
FatI CATG 4 cut(s) 346, 367, 427, 712
FauI CCCGC 2 cut(s) 56, 401
FbaI TGATCA 1 cut(s) 343
FblI GTMKAC 1 cut(s) 315
Fnu4HI GCNGC 6 cut(s) 61, 109, 397, 567, 570, 573
FokI GGATG 1 cut(s) 376
Fsp4HI GCNGC 6 cut(s) 61, 109, 397, 567, 570, 573
FspBI CTAG 1 cut(s) 498
FspI TGCGCA 1 cut(s) 37
GlaI GCGC 2 cut(s) 37, 173
GluI GCNGC 6 cut(s) 61, 109, 397, 567, 570, 573
GsaI CCCAGC 1 cut(s) 566
HaeIII GGCC 1 cut(s) 462
HapII CCGG 1 cut(s) 384
HgaI GACGC 1 cut(s) 556
HhaI GCGC 2 cut(s) 38, 174
Hin1II CATG 4 cut(s) 350, 371, 431, 716
Hin6I GCGC 2 cut(s) 36, 172
HinP1I GCGC 2 cut(s) 36, 172
HincII GTYRAC 1 cut(s) 590
HindII GTYRAC 1 cut(s) 590
HpaII CCGG 1 cut(s) 384
HphI GGTGA 3 cut(s) 245, 314, 636
Hpy166II GTNNAC 2 cut(s) 316, 590
Hpy188I TCNGA 2 cut(s) 238, 270
Hpy188III TCNNGA 5 cut(s) 14, 341, 347, 439, 544
Hpy8I GTNNAC 2 cut(s) 316, 590
HpyAV CCTTC 3 cut(s) 43, 275, 645
HpyCH4V TGCA 2 cut(s) 399, 456
HpyF10VI GCNNNNNNNGC 2 cut(s) 105, 572
Hsp92II CATG 4 cut(s) 350, 371, 431, 716
HspAI GCGC 2 cut(s) 36, 172
Ksp22I TGATCA 1 cut(s) 343
Kzo9I GATC 5 cut(s) 308, 343, 349, 386, 430
Lsp1109I GCAGC 6 cut(s) 72, 120, 383, 553, 556, 559
MaeI CTAG 1 cut(s) 498
MaeIII GTNAC 4 cut(s) 52, 251, 302, 362
MalI GATC 5 cut(s) 310, 345, 351, 388, 432
MboI GATC 5 cut(s) 308, 343, 349, 386, 430
MboII GAAGA 2 cut(s) 273, 457
MflI RGATCY 1 cut(s) 308
MluCI AATT 6 cut(s) 129, 245, 409, 555, 702, 707
MnlI CCTC 4 cut(s) 139, 414, 473, 657
MseI TTAA 3 cut(s) 408, 522, 705
MspA1I CMGCKG 3 cut(s) 63, 396, 566
MspI CCGG 1 cut(s) 384
MspR9I CCNGG 1 cut(s) 384
MvnI CGCG 1 cut(s) 174
MwoI GCNNNNNNNGC 2 cut(s) 105, 572
NciI CCSGG 1 cut(s) 384
NcoI CCATGG 2 cut(s) 367, 712
NdeII GATC 5 cut(s) 308, 343, 349, 386, 430
NlaIII CATG 4 cut(s) 350, 371, 431, 716
NlaIV GGNNCC 1 cut(s) 272
NmuCI GTSAC 2 cut(s) 251, 302
NsbI TGCGCA 1 cut(s) 37
PagI TCATGA 1 cut(s) 346
PflMI CCANNNNNTGG 1 cut(s) 219
PkrI GCNGC 6 cut(s) 62, 110, 398, 568, 571, 574
PspFI CCCAGC 1 cut(s) 562
PspN4I GGNNCC 1 cut(s) 272
PspPI GGNCC 1 cut(s) 193
PstNI CAGNNNCTG 1 cut(s) 569
PsuI RGATCY 1 cut(s) 308
PvuII CAGCTG 1 cut(s) 566
RsaI GTAC 2 cut(s) 88, 496
RsaNI GTAC 2 cut(s) 87, 495
SaqAI TTAA 3 cut(s) 408, 522, 705
SatI GCNGC 6 cut(s) 61, 109, 397, 567, 570, 573
Sau3AI GATC 5 cut(s) 308, 343, 349, 386, 430
Sau96I GGNCC 1 cut(s) 193
ScaI AGTACT 1 cut(s) 496
ScrFI CCNGG 1 cut(s) 384
SetI ASST 7 cut(s) 54, 75, 184, 321, 568, 649, 660
SfcI CTRYAG 1 cut(s) 82
SinI GGWCC 1 cut(s) 193
SmlI CTYRAG 1 cut(s) 668
SmoI CTYRAG 1 cut(s) 668
SpeI ACTAGT 1 cut(s) 497
Sse9I AATT 6 cut(s) 129, 245, 409, 555, 702, 707
SsiI CCGC 5 cut(s) 63, 196, 256, 291, 394
SspMI CTAG 1 cut(s) 498
StyD4I CCNGG 1 cut(s) 382
StyI CCWWGG 2 cut(s) 367, 712
TaqI TCGA 2 cut(s) 184, 438
TasI AATT 6 cut(s) 129, 245, 409, 555, 702, 707
TatI WGTACW 2 cut(s) 86, 494
Tru1I TTAA 3 cut(s) 408, 522, 705
Tru9I TTAA 3 cut(s) 408, 522, 705
TseFI GTSAC 2 cut(s) 251, 302
TseI GCWGC 6 cut(s) 60, 108, 396, 566, 569, 572
Tsp45I GTSAC 2 cut(s) 251, 302
TspGWI ACGGA 2 cut(s) 158, 301
Van91I CCANNNNNTGG 1 cut(s) 219
VpaK11BI GGWCC 1 cut(s) 193
XapI RAATTY 2 cut(s) 245, 707
XcmI CCANNNNNNNNNTGG 1 cut(s) 455
XmiI GTMKAC 1 cut(s) 315
XspI CTAG 1 cut(s) 498
ZrmI AGTACT 1 cut(s) 496
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.