MD17G1178000.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0008474
Physical Location & Seq
Forward (+)
20374366 .. 20374713
348 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1178000.v1.1.491

Sequence Viewer

Length: 348 bp
ATGAGAGCCGAGCTTCCCCCAAGAAGAAGCAATAGTTACCCAAGGACGCGACCGTATTCCAAGCCACTAGGCACAGCTAGTAGCAGTACTACTGCACAAGCACCACAACAAGCAATGAGCGCAAGACAACTTAGTACCGATCAAGGAATCAGAAGAGTGTTTCGGGGAAAGTCATTGACCGACGACGTCTTGATGAGAAGGTTTGTTGTAGAAGAGGAGGCAATGATGCAAGTTAGAAGGAGAACTCAAATGGAAGTCATAAGGAGGAGAAGTGTGATGAGGAGGAAGAGGCTTGGCCCCAGTCCTCTTTGTAGAATGGCTGTGGCTCAGGTTCGGGAGGAGGTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

116

Amino Acids

13.32

Weight (kDa)

11.76

Isoelectric Point (pI)

85.52

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 189
AccII CGCG 1 cut(s) 49
AcyI GRCGYC 1 cut(s) 186
AfaI GTAC 2 cut(s) 88, 136
AluBI AGCT 2 cut(s) 13, 77
AluI AGCT 2 cut(s) 13, 77
AoxI GGCC 1 cut(s) 295
AspLEI GCGC 1 cut(s) 122
AspS9I GGNCC 1 cut(s) 296
BfaI CTAG 2 cut(s) 68, 78
BmcAI AGTACT 1 cut(s) 88
BmgT120I GGNCC 1 cut(s) 296
BmiI GGNNCC 1 cut(s) 298
BmrI ACTGGG 1 cut(s) 294
BmsI GCATC 1 cut(s) 216
BmuI ACTGGG 1 cut(s) 294
Bpu10I CCTNAGC 1 cut(s) 327
BsaHI GRCGYC 1 cut(s) 186
BsaJI CCNNGG 1 cut(s) 41
BsaXI ACNNNNNCTCC 2 cut(s) 256, 286
Bse1I ACTGG 1 cut(s) 300
Bse3DI GCAATG 2 cut(s) 120, 228
BseDI CCNNGG 1 cut(s) 41
BseMI GCAATG 2 cut(s) 120, 228
BseMII CTCAG 1 cut(s) 341
BseNI ACTGG 1 cut(s) 300
BseRI GAGGAG 3 cut(s) 230, 280, 295
BsgI GTGCAG 1 cut(s) 78
Bsh1236I CGCG 1 cut(s) 49
Bsh1285I CGRYCG 1 cut(s) 53
BshFI GGCC 1 cut(s) 297
BsiEI CGRYCG 1 cut(s) 53
BsnI GGCC 1 cut(s) 297
Bsp143I GATC 1 cut(s) 139
BspANI GGCC 1 cut(s) 297
BspCNI CTCAG 1 cut(s) 340
BspFNI CGCG 1 cut(s) 49
BspLI GGNNCC 1 cut(s) 298
BsrDI GCAATG 2 cut(s) 120, 228
BsrI ACTGG 1 cut(s) 300
BssECI CCNNGG 1 cut(s) 41
BssMI GATC 1 cut(s) 139
BssNI GRCGYC 1 cut(s) 186
BssT1I CCWWGG 1 cut(s) 41
Bst4CI ACNGT 1 cut(s) 54
Bst6I CTCTTC 3 cut(s) 148, 207, 281
BstACI GRCGYC 1 cut(s) 186
BstDEI CTNAG 2 cut(s) 131, 327
BstFNI CGCG 1 cut(s) 49
BstHHI GCGC 1 cut(s) 122
BstKTI GATC 1 cut(s) 142
BstMBI GATC 1 cut(s) 139
BstMCI CGRYCG 1 cut(s) 53
BstMWI GCNNNNNNNGC 1 cut(s) 119
BstUI CGCG 1 cut(s) 49
BsuRI GGCC 1 cut(s) 297
CfoI GCGC 1 cut(s) 122
Cfr13I GGNCC 1 cut(s) 296
CseI GACGC 1 cut(s) 55
Csp6I GTAC 2 cut(s) 87, 135
CviJI RGCY 8 cut(s) 8, 13, 64, 77, 292, 297, 320, 326
CviKI_1 RGCY 8 cut(s) 8, 13, 64, 77, 292, 297, 320, 326
CviQI GTAC 2 cut(s) 87, 135
DdeI CTNAG 2 cut(s) 131, 327
DpnI GATC 1 cut(s) 141
DpnII GATC 1 cut(s) 139
Eam1104I CTCTTC 3 cut(s) 148, 207, 281
EarI CTCTTC 3 cut(s) 148, 207, 281
Eco130I CCWWGG 1 cut(s) 41
EcoT14I CCWWGG 1 cut(s) 41
ErhI CCWWGG 1 cut(s) 41
FaiI YATR 1 cut(s) 260
FspBI CTAG 2 cut(s) 68, 78
GlaI GCGC 1 cut(s) 121
HaeIII GGCC 1 cut(s) 297
HgaI GACGC 1 cut(s) 55
HhaI GCGC 1 cut(s) 122
Hin1I GRCGYC 1 cut(s) 186
Hin6I GCGC 1 cut(s) 120
HinP1I GCGC 1 cut(s) 120
HinfI GANTC 1 cut(s) 147
Hpy188I TCNGA 1 cut(s) 152
Hpy188III TCNNGA 2 cut(s) 190, 335
Hpy99I CGWCG 2 cut(s) 185, 188
HpyAV CCTTC 2 cut(s) 192, 231
HpyCH4III ACNGT 1 cut(s) 54
HpyCH4IV ACGT 1 cut(s) 186
HpyCH4V TGCA 2 cut(s) 95, 229
HpyF10VI GCNNNNNNNGC 1 cut(s) 119
HpyF3I CTNAG 2 cut(s) 131, 327
HpySE526I ACGT 1 cut(s) 186
Hsp92I GRCGYC 1 cut(s) 186
HspAI GCGC 1 cut(s) 120
Kzo9I GATC 1 cut(s) 139
LpnPI CCDG 2 cut(s) 313, 314
LweI GCATC 1 cut(s) 216
MaeI CTAG 2 cut(s) 68, 78
MaeII ACGT 1 cut(s) 186
MaeIII GTNAC 1 cut(s) 35
MalI GATC 1 cut(s) 141
MboI GATC 1 cut(s) 139
MboII GAAGA 4 cut(s) 36, 165, 224, 298
MnlI CCTC 9 cut(s) 208, 211, 258, 273, 276, 282, 315, 331, 334
MseI TTAA 1 cut(s) 346
MvnI CGCG 1 cut(s) 49
MwoI GCNNNNNNNGC 1 cut(s) 119
NdeII GATC 1 cut(s) 139
NlaIV GGNNCC 1 cut(s) 298
NmeAIII GCCGAG 1 cut(s) 34
PfeI GAWTC 1 cut(s) 147
PflFI GACNNNGTC 1 cut(s) 185
PspN4I GGNNCC 1 cut(s) 298
PspPI GGNCC 1 cut(s) 296
PsyI GACNNNGTC 1 cut(s) 185
RsaI GTAC 2 cut(s) 88, 136
RsaNI GTAC 2 cut(s) 87, 135
SaqAI TTAA 1 cut(s) 346
Sau3AI GATC 1 cut(s) 139
Sau96I GGNCC 1 cut(s) 296
ScaI AGTACT 1 cut(s) 88
SetI ASST 6 cut(s) 15, 79, 189, 203, 333, 345
SfaNI GCATC 1 cut(s) 216
SspMI CTAG 2 cut(s) 68, 78
StyI CCWWGG 1 cut(s) 41
TaaI ACNGT 1 cut(s) 54
TaiI ACGT 1 cut(s) 189
TaqII GACCGA 1 cut(s) 194
TatI WGTACW 1 cut(s) 86
TfiI GAWTC 1 cut(s) 147
Tru1I TTAA 1 cut(s) 346
Tru9I TTAA 1 cut(s) 346
Tth111I GACNNNGTC 1 cut(s) 185
XspI CTAG 2 cut(s) 68, 78
ZraI GACGTC 1 cut(s) 187
ZrmI AGTACT 1 cut(s) 88
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.