MD04G1049500.v1.1

Autophagy-related protein 11

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0008019
Physical Location & Seq
Reverse (-)
5790665 .. 5798158
7494 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1049500.v1.1.491

Sequence Viewer

Length: 3441 bp
ATGAGTTCGAGTATCGCTGAAGGTTTGGTTCATCAGGGCAAGCTTTTGGTTCATGTTGCTGAGAATGGGCATTCATTTGAGCTCGATTGTGAAGACAGCACACCGGTGGAGGCAGTTATGCTGTATATTGAATCCATGGCTGGGATTAATCTCAATGACCAGCTTGTTTTGTGTTTGGATATGAAGCTTGAGCCACAGAGGCCGCTTTCGGCATATAAGCTTCCAGCTGATGGTCTGGAAGTGTTTATATTCAATAAAGCAAGACTGCAGACTAATTCTCCACTCCCTCAGCCCGAGCAAGTTGAAATTCTTGACATTGCTGACCCCCAATCACCTTCAGCTTCACATGACCCTCACCCTTTGGATGATGTTTCAGACCCTGCTTTGAAGGCCTTGCCTTCTTATGAAAGGCAATTTAGGTACCACTACCACAAGGGTCATGCAATTTACAGTTGCACCCAAGTAAAATATGAGAACTGTGAGAGGCTTTTGAGGGAGCAAAAGGTTCAAGAAAGGGCAGTGGAGGTTGCTAGGGGCAATTTGGATCAATATTATAAAATGATAAACCAAAACTATACAGAATTCATGAAGCGTTATTCACAACAGCATCGAAGTCATTCTGATCTTTTGACCAATCTTGGGAGGGATGTAGAAAAACTGAGATCTATCAAGCTTCACCCTGCATTAGAGACTGCCACTCGGAAGTGCTTATTGGATTTTGTGAAGGAAGAGAACTTGAGGAAAGCGGGAGAAATTTGTAGTAGTTCCCACAGGCAGTTTGAGAATAAGGTTTCACAGTTTAAACAGATGTTTAGTGAGGTAAAGCGGAAAGTCGAAGAATTGTTTTCTAATAGGGCTTCGTTGCCTATTAGGAATTTGGAAATCACGATTAAGGAGCACCAAAGATATATTCATGAGCAAAGGAGTATCATGCAATCATTGAGCAAAGATGTAAATACTGTCAAGAAACTCGTGGATGATTGCTTGTCTTGCCAATTGTCTTCCTCACTTCGTCCTCATGATGCAGTTTCAGCCTTGGGCCCTATGTATGATGTCCATGACAAAAATCACCTGCCTAGGATGCAGGCTTGTGATCGCGTAATTTCCAAGCTCCTCAACTTCTGCAAGGATAAAAAGAATGAAATGAATGTATTTCTCCATAGATTTATGCAAAAGATAACATATATTTCTTACATCATCAAAGATGCAAAATTACAGTTTCCTGTTTTTAGAGAGGCAATGGTCCGTCAGGAGGATCTATTTTTGGACTTAAAGTTGGTCCGTGGGATCGGCCCTGCATATAGAGCCTGCTTAGCAGAAATAGTCAGACGAAAGGCTTCATTGAAGCTTTACATGGGGATGGCTGGACAGTTGGCTGAAAGACTTGCGACAAAGAGGGAGACTGAAGTCAGGAGACGGGAGGAGTTTTTGAGAGCTCACAGTTTATACATACCCAGGGATGTATTAGCATCCATGGGACTATATGATACGCCTAATCAGTGTGATGTCAACATAGCTCCTTTTGATACTAGCTTGCTTGATATTGAGATTTCAGACATTGACCGTTATGCACCTGAGTACTTGACTGCACTGTCTTCAAAGAGTGCGTTTAGAGGTTCAAATTCTATGTCTAATGAAAGTCATTCGGTTGATGCTGATGAGAGTACATTGGATAACTTTGAGAATTGCAACTCTGAGGAGCTACTTGAAGGCTGTGAGTTGGTAGAGATTGCTGGAACTGGTAAGTTGGAAGTTGAGAATGCAAAACTGAAAGCAGAACTGGCGTCTGCTATAGCTGTGATTTGTTCATTCTGGCCTGAAGTAGACTTTGAATCATTGGATGATAGTAAAGTGGATCATTTATTGAAGGATGCCGCAGAGAAGACAGCTGAAGCCTTGCACCTGAAAGATGAATATGGAAAGCATCTAAAATCTATGCTCAGGGAGAAGCAGATGCAGTGTCTTTCATATGAGAAACGCATTCAAGAACTTGAACAGAGATTGTCTGATCAGTATTCACAAAGCCAGAAGATTTCAAATGATAAGGATGCCTCTGAATTTGGAATTTTGTCTGATAAGGTTGATATCTGCAAGCAAGAAGGTGGTAGGGGAGTCCATATGCCTTGCTCATCTCATACTGATCCCATGGATGAGGTTTCCTGCGTCTCTAGTGTTTTTGATGCAAAACTGGGTCTGTTCAATGTGCAACCTGGGAAAATGAGAGATGGGGTAGATGAAAATATGATGGATTCTTCTGCAGTACGAAATCATCTAATGGATTCATCCATGCAAGAGCTACAGCGTGAAGAACTGCTAGCCAGTGGTAAAGATGGGAAAGATAAGATGGAGGGGCAGCTGGGCATGTCACTGACAAACAGTTCTACTGCTGAAAGCATGCCTGAACCTCTTAATGTTATACCATGTGAAACAGCAGTTGACCCAGGCTTGGACACCAAAGTCAGTGCGGAGCTGTTACTGGAATTGCAAACTGCACTTTCAGAGAAGTCAAACCAGTTGAGTGAAACAGAAATTAAGCTTAAAGCTGCTATGGAGGATGTTTCTATGCTTAAGAGGGAATTGGACACAAATCGGAAACTCCTTGATGAATCTCAGATGAATTGTGCGCATTTGGAGAATTGTTTGCATGAGGCAAGAGAGGAAGCTCAAACCCATCTTTGTGCGGCTGATCGTAGGGCCTCGGAGTATAGTGCACTGCGCGCATCTGCTGTGAAAATGCGTGGCCTTTTTGAAAGACTTAGGAGCTGTGTATATGCCCAGGGTGGGGTGGCTAGTTTTAATGAATCTTTGCGCACCTTAGCACAATCTCTGGGCAATTCTATTAACGACAATGAAGATGATGGTACTGTTGAATTTCGGAAATGTATTCGGGTCCTGGCAGATAGAGTTGGCTTCTTGTCAAGGCACCGTGAAGAGCTACTTGACAAGTATCCAAAGGTTGAAGCTGCAAATGAACAACTTAGAAAAGAATTGGAAGAGAAGAAGGAGCTGGTTAAAACATTGTACACAAAGCATCAACTCGAGAAGCAGGCAAACAAAGAGAAGATATCTTTCGGTCGCTTGGAAGTCCACGAGATTGCTGCATTTGTTCTAAACACATTTGGGCAGTACGAGGCAATTAATCGGAATTGCTCCAACTACTACCTGTCTGCTGAATCTGTAGCCTTGTTTACCGATCATCTCCCAAATCAACCTAACTACATTGTTGGACAAATTGTGCACATCGAACGGCAGACTGTGAAGCCAATGGCTCCTAGTTCAACCCGGTCTGAGCACGAGCTAACATCTGACACGGGAACCGACCGTTTGGCCTTGAATTCAGGATTGAACCCGTATGGTCTCCCTATTGGCTGTGAATACTTCGTAGTGACAGTAGCCATGTTACCTGGTACCACCACCATTCATACCCCGCCTCCTTCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000045 GO:0000272 GO:0000407 GO:0000422 GO:0001932 GO:0001934 GO:0003674 GO:0005198 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005776 GO:0005911 GO:0005975 GO:0005976 GO:0005977 GO:0005980 GO:0006073 GO:0006091 GO:0006112 GO:0006914 GO:0006996 GO:0007005 GO:0007033 GO:0007275 GO:0007568 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009251 GO:0009506 GO:0009893 GO:0009987 GO:0010150 GO:0010562 GO:0010604 GO:0015980 GO:0016020 GO:0016043 GO:0016052 GO:0016236 GO:0019220 GO:0019222 GO:0019899 GO:0019900 GO:0019901 GO:0022411 GO:0022607 GO:0030054 GO:0030242 GO:0031323 GO:0031325 GO:0031399 GO:0031401 GO:0032268 GO:0032270 GO:0032501 GO:0032502 GO:0032947 GO:0032991 GO:0034045 GO:0042325 GO:0042327 GO:0042802 GO:0042803 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044042 GO:0044085 GO:0044237 GO:0044238 GO:0044247 GO:0044248 GO:0044260 GO:0044262 GO:0044264 GO:0044275 GO:0044424 GO:0044444 GO:0044464 GO:0045937 GO:0046983 GO:0048366 GO:0048367 GO:0048518 GO:0048522 GO:0048731 GO:0048827 GO:0048856 GO:0050789 GO:0050794 GO:0051171 GO:0051173 GO:0051174 GO:0051246 GO:0051247 GO:0055044 GO:0055114 GO:0060255 GO:0061695 GO:0061709 GO:0061723 GO:0061726 GO:0061912 GO:0061919 GO:0065007 GO:0070925 GO:0071704 GO:0071840 GO:0080090 GO:0090693 GO:0098805 GO:0099402 GO:1901575 GO:1902494 GO:1902554 GO:1902911 GO:1903008 GO:1905037 GO:1990234 GO:1990316
KEGG Pathways
Metabolic & Signaling
ko04136 ko04138 ko04139 map04136 map04138 map04139
Pfam Domains
Protein Families
APG17 ATG11

Protein Analysis

1147

Amino Acids

129.76

Weight (kDa)

5.61

Isoelectric Point (pI)

41.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ATG17_like PF04108 150 - 483 2.8e-32 Autophagy protein ATG17-like domain
ATG11 PF10377 991 - 1133 5.6e-20 Autophagy-related protein 11
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 555
AarI CACCTGC 1 cut(s) 1080
AasI GACNNNNNNGTC 2 cut(s) 1591, 2456
Acc16I TGCGCA 2 cut(s) 2625, 2810
Acc36I ACCTGC 1 cut(s) 1080
Acc65I GGTACC 2 cut(s) 420, 3407
AccB1I GGYRCC 3 cut(s) 420, 2922, 3407
AccB7I CCANNNNNTGG 1 cut(s) 230
AccI GTMKAC 1 cut(s) 1824
AccII CGCG 2 cut(s) 1098, 2718
AciI CCGC 7 cut(s) 203, 746, 826, 1875, 2465, 2681, 3428
AclWI GGATC 5 cut(s) 552, 1263, 1295, 1863, 2135
AcsI RAATTY 9 cut(s) 306, 581, 753, 874, 1621, 2057, 2064, 2870, 3334
AcuI CTGAAG 5 cut(s) 39, 321, 1425, 1839, 1911
AcyI GRCGYC 1 cut(s) 1784
AfaI GTAC 8 cut(s) 422, 1580, 1666, 2262, 2863, 3023, 3128, 3409
AfiI CCNNNNNNNGG 7 cut(s) 141, 230, 639, 1252, 2446, 2781, 2782
AflII CTTAAG 1 cut(s) 2567
AgeI ACCGGT 1 cut(s) 103
AjnI CCWGG 6 cut(s) 1454, 2209, 2440, 2775, 2892, 3403
AjuI GAANNNNNNNTTGG 4 cut(s) 695, 727, 894, 926
AleI CACNNNNGTG 1 cut(s) 104
Alw21I GWGCWC 6 cut(s) 84, 900, 1438, 2713, 3240, 3294
Alw26I GTCTC 5 cut(s) 683, 1394, 1408, 2170, 3362
Alw44I GTGCAC 2 cut(s) 2709, 3236
AlwI GGATC 5 cut(s) 552, 1263, 1295, 1863, 2135
AlwNI CAGNNNCTG 1 cut(s) 380
Ama87I CYCGRG 2 cut(s) 293, 3038
AoxI GGCC 8 cut(s) 200, 390, 1039, 1291, 1814, 2694, 2740, 3327
ApaI GGGCCC 1 cut(s) 1043
ApaLI GTGCAC 2 cut(s) 2709, 3236
ApeKI GCWGC 4 cut(s) 2353, 2543, 2963, 3098
ApoI RAATTY 9 cut(s) 306, 581, 753, 874, 1621, 2057, 2064, 2870, 3334
ArsI GACNNNNNNTTYG 2 cut(s) 1613, 1645
AseI ATTAAT 2 cut(s) 147, 3138
AsiGI ACCGGT 1 cut(s) 103
Asp700I GAANNNNTTC 2 cut(s) 616, 1336
Asp718I GGTACC 2 cut(s) 420, 3407
AspA2I CCTAGG 1 cut(s) 1076
AspLEI GCGC 4 cut(s) 2626, 2718, 2720, 2811
AspS9I GGNCC 7 cut(s) 1039, 1040, 1243, 1279, 1292, 2694, 2890
AsuC2I CCSGG 1 cut(s) 3283
AsuHPI GGTGA 4 cut(s) 324, 347, 668, 1061
AsuNHI GCTAGC 1 cut(s) 2314
AvaI CYCGRG 2 cut(s) 293, 3038
AvaII GGWCC 3 cut(s) 1243, 1279, 2890
AvrII CCTAGG 1 cut(s) 1076
BaeGI GKGCMC 3 cut(s) 1043, 2713, 3240
BanI GGYRCC 3 cut(s) 420, 2922, 3407
BanII GRGCYC 3 cut(s) 84, 1043, 1438
BauI CACGAG 3 cut(s) 971, 3089, 3293
BbsI GAAGAC 4 cut(s) 99, 993, 1587, 1889
Bbv12I GWGCWC 6 cut(s) 84, 900, 1438, 2713, 3240, 3294
BbvCI CCTCAGC 1 cut(s) 288
BbvI GCAGC 4 cut(s) 2365, 2530, 2950, 3085
BccI CCATC 8 cut(s) 224, 1354, 2219, 2239, 2324, 2338, 2679, 2852
BceAI ACGGC 1 cut(s) 3263
BcgI CGANNNNNNTGC 2 cut(s) 3080, 3114
BciT130I CCWGG 6 cut(s) 1456, 2211, 2442, 2777, 2894, 3405
BciVI GTATCC 1 cut(s) 2958
BclI TGATCA 1 cut(s) 2008
BcnI CCSGG 1 cut(s) 3283
BcoDI GTCTC 5 cut(s) 683, 1394, 1408, 2170, 3362
BfaI CTAG 7 cut(s) 531, 1077, 1530, 2169, 2315, 2790, 3273
BfmI CTRYAG 5 cut(s) 266, 1791, 2256, 2297, 3177
BfrI CTTAAG 1 cut(s) 2567
BfuAI ACCTGC 1 cut(s) 1080
BfuI GTATCC 1 cut(s) 2958
BglI GCCNNNNNGGC 1 cut(s) 199
BglII AGATCT 1 cut(s) 662
BisI GCNGC 7 cut(s) 203, 1875, 2354, 2544, 2682, 2964, 3099
BlnI CCTAGG 1 cut(s) 1076
BlpI GCTNAGC 1 cut(s) 1312
BlsI GCNGC 7 cut(s) 204, 1876, 2355, 2545, 2683, 2965, 3100
BmcAI AGTACT 1 cut(s) 1580
Bme1390I CCNGG 7 cut(s) 1456, 2211, 2442, 2777, 2894, 3283, 3405
Bme18I GGWCC 3 cut(s) 1243, 1279, 2890
BmeT110I CYCGRG 2 cut(s) 293, 3038
BmgT120I GGNCC 7 cut(s) 1039, 1040, 1243, 1279, 1292, 2694, 2890
BmiI GGNNCC 7 cut(s) 422, 1041, 2891, 2924, 3270, 3316, 3409
BmrFI CCNGG 7 cut(s) 1456, 2211, 2442, 2777, 2894, 3283, 3405
BmrI ACTGGG 1 cut(s) 2198
BmtI GCTAGC 1 cut(s) 2318
BmuI ACTGGG 1 cut(s) 2198
BoxI GACNNNNGTC 1 cut(s) 1406
BpiI GAAGAC 4 cut(s) 99, 993, 1587, 1889
Bpu10I CCTNAGC 3 cut(s) 288, 1940, 2815
Bpu1102I GCTNAGC 1 cut(s) 1312
BpuEI CTTGAG 2 cut(s) 209, 757
BpuMI CCSGG 1 cut(s) 3283
BsaHI GRCGYC 1 cut(s) 1784
BsaI GGTCTC 1 cut(s) 3362
BsaWI WCCGGW 1 cut(s) 103
BsaXI ACNNNNNCTCC 3 cut(s) 262, 292, 3415
Bsc4I CCNNNNNNNGG 7 cut(s) 141, 230, 639, 1252, 2446, 2781, 2782
Bse118I RCCGGY 1 cut(s) 103
Bse1I ACTGG 6 cut(s) 1744, 1785, 2193, 2319, 2481, 2512
Bse3DI GCAATG 2 cut(s) 315, 1245
BseBI CCWGG 6 cut(s) 1456, 2211, 2442, 2777, 2894, 3405
BseLI CCNNNNNNNGG 7 cut(s) 141, 230, 639, 1252, 2446, 2781, 2782
BseMI GCAATG 2 cut(s) 315, 1245
BseMII CTCAG 8 cut(s) 51, 302, 650, 1566, 1686, 1954, 2624, 3279
BseNI ACTGG 6 cut(s) 1744, 1785, 2193, 2319, 2481, 2512
BsePI GCGCGC 1 cut(s) 2716
BseRI GAGGAG 3 cut(s) 1103, 1436, 1712
BseSI GKGCMC 3 cut(s) 1043, 2713, 3240
BseXI GCAGC 4 cut(s) 2365, 2530, 2950, 3085
BseYI CCCAGC 2 cut(s) 140, 2356
BsgI GTGCAG 2 cut(s) 1572, 2475
Bsh1236I CGCG 2 cut(s) 1098, 2718
Bsh1285I CGRYCG 2 cut(s) 3076, 3322
BshFI GGCC 8 cut(s) 202, 392, 1041, 1293, 1816, 2696, 2742, 3329
BshNI GGYRCC 3 cut(s) 420, 2922, 3407
BshTI ACCGGT 1 cut(s) 103
BsiEI CGRYCG 2 cut(s) 3076, 3322
BsiHKAI GWGCWC 6 cut(s) 84, 900, 1438, 2713, 3240, 3294
BsiHKCI CYCGRG 2 cut(s) 293, 3038
BsiSI CCGG 2 cut(s) 104, 3283
BslFI GGGAC 1 cut(s) 1491
BslI CCNNNNNNNGG 7 cut(s) 141, 230, 639, 1252, 2446, 2781, 2782
BsmAI GTCTC 5 cut(s) 683, 1394, 1408, 2170, 3362
BsmBI CGTCTC 2 cut(s) 1408, 2170
BsmFI GGGAC 1 cut(s) 1491
BsmI GAATGC 3 cut(s) 70, 1765, 1980
BsnI GGCC 8 cut(s) 202, 392, 1041, 1293, 1816, 2696, 2742, 3329
Bso31I GGTCTC 1 cut(s) 3362
BsoBI CYCGRG 2 cut(s) 293, 3038
Bsp120I GGGCCC 1 cut(s) 1039
Bsp1286I GDGCHC 7 cut(s) 84, 900, 1043, 1438, 2713, 3240, 3294
Bsp1407I TGTACA 1 cut(s) 3021
Bsp1720I GCTNAGC 1 cut(s) 1312
Bsp19I CCATGG 3 cut(s) 135, 1473, 2145
BspACI CCGC 7 cut(s) 203, 746, 826, 1875, 2465, 2681, 3428
BspANI GGCC 8 cut(s) 202, 392, 1041, 1293, 1816, 2696, 2742, 3329
BspCNI CTCAG 8 cut(s) 52, 301, 651, 1567, 1687, 1953, 2623, 3280
BspFNI CGCG 2 cut(s) 1098, 2718
BspHI TCATGA 3 cut(s) 585, 913, 1018
BspLI GGNNCC 7 cut(s) 422, 1041, 2891, 2924, 3270, 3316, 3409
BspMAI CTGCAG 2 cut(s) 270, 2260
BspMI ACCTGC 1 cut(s) 1080
BspOI GCTAGC 1 cut(s) 2318
BspPI GGATC 5 cut(s) 552, 1263, 1295, 1863, 2135
BspQI GCTCTTC 1 cut(s) 2925
BspT107I GGYRCC 3 cut(s) 420, 2922, 3407
BspTI CTTAAG 1 cut(s) 2567
BspTNI GGTCTC 1 cut(s) 3362
BsrDI GCAATG 2 cut(s) 315, 1245
BsrFI RCCGGY 1 cut(s) 103
BsrGI TGTACA 1 cut(s) 3021
BsrI ACTGG 6 cut(s) 1744, 1785, 2193, 2319, 2481, 2512
BssAI RCCGGY 1 cut(s) 103
BssHII GCGCGC 1 cut(s) 2716
BssNI GRCGYC 1 cut(s) 1784
BssSI CACGAG 3 cut(s) 971, 3089, 3293
BssT1I CCWWGG 5 cut(s) 135, 1035, 1076, 1473, 2145
Bst2BI CACGAG 3 cut(s) 971, 3089, 3293
Bst2UI CCWGG 6 cut(s) 1456, 2211, 2442, 2777, 2894, 3405
Bst6I CTCTTC 3 cut(s) 723, 2925, 2988
BstACI GRCGYC 1 cut(s) 1784
BstAFI CTTAAG 1 cut(s) 2567
BstAUI TGTACA 1 cut(s) 3021
BstC8I GCNNGC 9 cut(s) 41, 1086, 1309, 1535, 2093, 2316, 2396, 2718, 3048
BstDSI CCRYGG 4 cut(s) 135, 1282, 1473, 2145
BstFNI CGCG 2 cut(s) 1098, 2718
BstHHI GCGC 4 cut(s) 2626, 2718, 2720, 2811
BstMAI GTCTC 5 cut(s) 683, 1394, 1408, 2170, 3362
BstMCI CGRYCG 2 cut(s) 3076, 3322
BstMWI GCNNNNNNNGC 9 cut(s) 199, 389, 990, 1031, 1081, 1304, 1313, 1781, 2717
BstNI CCWGG 6 cut(s) 1456, 2211, 2442, 2777, 2894, 3405
BstNSI RCATGY 2 cut(s) 2365, 2398
BstPAI GACNNNNGTC 1 cut(s) 1406
BstSCI CCNGG 7 cut(s) 1454, 2209, 2440, 2775, 2892, 3281, 3403
BstSFI CTRYAG 5 cut(s) 266, 1791, 2256, 2297, 3177
BstSLI GKGCMC 3 cut(s) 1043, 2713, 3240
BstUI CGCG 2 cut(s) 1098, 2718
BstV1I GCAGC 4 cut(s) 2365, 2530, 2950, 3085
BstV2I GAAGAC 4 cut(s) 99, 993, 1587, 1889
BstX2I RGATCY 2 cut(s) 662, 1255
BstYI RGATCY 2 cut(s) 662, 1255
BsuI GTATCC 1 cut(s) 2958
BsuRI GGCC 8 cut(s) 202, 392, 1041, 1293, 1816, 2696, 2742, 3329
BtgI CCRYGG 4 cut(s) 135, 1282, 1473, 2145
BtsI GCAGTG 3 cut(s) 525, 1964, 2711
BtsIMutI CAGTG 8 cut(s) 525, 1505, 1589, 1964, 2326, 2366, 2467, 2711
BveI ACCTGC 1 cut(s) 1080
Cac8I GCNNGC 9 cut(s) 41, 1086, 1309, 1535, 2093, 2316, 2396, 2718, 3048
CaiI CAGNNNCTG 1 cut(s) 380
CciI TCATGA 3 cut(s) 585, 913, 1018
CfoI GCGC 4 cut(s) 2626, 2718, 2720, 2811
Cfr10I RCCGGY 1 cut(s) 103
Cfr13I GGNCC 7 cut(s) 1039, 1040, 1243, 1279, 1292, 2694, 2890
CseI GACGC 2 cut(s) 1773, 2152
CsiI ACCWGGT 1 cut(s) 3403
Csp6I GTAC 8 cut(s) 421, 1579, 1665, 2261, 2862, 3022, 3127, 3408
CspAI ACCGGT 1 cut(s) 103
CviQI GTAC 8 cut(s) 421, 1579, 1665, 2261, 2862, 3022, 3127, 3408
DraI TTTAAA 1 cut(s) 802
DrdI GACNNNNNNGTC 2 cut(s) 1591, 2456
DseDI GACNNNNNNGTC 2 cut(s) 1591, 2456
Eam1104I CTCTTC 3 cut(s) 723, 2925, 2988
EarI CTCTTC 3 cut(s) 723, 2925, 2988
Ecl136II GAGCTC 2 cut(s) 82, 1436
Eco130I CCWWGG 5 cut(s) 135, 1035, 1076, 1473, 2145
Eco147I AGGCCT 1 cut(s) 392
Eco24I GRGCYC 3 cut(s) 84, 1043, 1438
Eco31I GGTCTC 1 cut(s) 3362
Eco32I GATATC 2 cut(s) 2086, 3066
Eco47I GGWCC 3 cut(s) 1243, 1279, 2890
Eco53kI GAGCTC 2 cut(s) 82, 1436
Eco57I CTGAAG 5 cut(s) 39, 321, 1425, 1839, 1911
Eco88I CYCGRG 2 cut(s) 293, 3038
EcoICRI GAGCTC 2 cut(s) 82, 1436
EcoO109I RGGNCCY 3 cut(s) 1040, 2694, 2890
EcoRI GAATTC 2 cut(s) 581, 3334
EcoRII CCWGG 6 cut(s) 1454, 2209, 2440, 2775, 2892, 3403
EcoRV GATATC 2 cut(s) 2086, 3066
EcoT14I CCWWGG 5 cut(s) 135, 1035, 1076, 1473, 2145
EcoT38I GRGCYC 3 cut(s) 84, 1043, 1438
ErhI CCWWGG 5 cut(s) 135, 1035, 1076, 1473, 2145
Esp3I CGTCTC 2 cut(s) 1408, 2170
FalI AAGNNNNNCTT 2 cut(s) 2922, 2954
FaqI GGGAC 1 cut(s) 1491
FauI CCCGC 2 cut(s) 739, 3435
FauNDI CATATG 2 cut(s) 1969, 2118
FbaI TGATCA 1 cut(s) 2008
FblI GTMKAC 1 cut(s) 1824
Fnu4HI GCNGC 7 cut(s) 203, 1875, 2354, 2544, 2682, 2964, 3099
FriOI GRGCYC 3 cut(s) 84, 1043, 1438
Fsp4HI GCNGC 7 cut(s) 203, 1875, 2354, 2544, 2682, 2964, 3099
FspAI RTGCGCAY 1 cut(s) 2625
FspBI CTAG 7 cut(s) 531, 1077, 1530, 2169, 2315, 2790, 3273
FspI TGCGCA 2 cut(s) 2625, 2810
GlaI GCGC 4 cut(s) 2625, 2717, 2719, 2810
GluI GCNGC 7 cut(s) 203, 1875, 2354, 2544, 2682, 2964, 3099
GsaI CCCAGC 2 cut(s) 144, 2360
HaeIII GGCC 8 cut(s) 202, 392, 1041, 1293, 1816, 2696, 2742, 3329
HapII CCGG 2 cut(s) 104, 3283
HgaI GACGC 2 cut(s) 1773, 2152
HhaI GCGC 4 cut(s) 2626, 2718, 2720, 2811
Hin1I GRCGYC 1 cut(s) 1784
Hin6I GCGC 4 cut(s) 2624, 2716, 2718, 2809
HinP1I GCGC 4 cut(s) 2624, 2716, 2718, 2809
HincII GTYRAC 2 cut(s) 1510, 2437
HindII GTYRAC 2 cut(s) 1510, 2437
HindIII AAGCTT 6 cut(s) 41, 185, 218, 671, 1346, 2534
HinfI GANTC 8 cut(s) 131, 1832, 2112, 2249, 2279, 2606, 2801, 3173
HpaII CCGG 2 cut(s) 104, 3283
HphI GGTGA 4 cut(s) 324, 347, 668, 1061
Hpy166II GTNNAC 8 cut(s) 1510, 1825, 2437, 2711, 3024, 3088, 3189, 3238
Hpy8I GTNNAC 8 cut(s) 1510, 1825, 2437, 2711, 3024, 3088, 3189, 3238
HpyAV CCTTC 9 cut(s) 14, 345, 382, 408, 718, 1703, 1861, 2093, 2995
HpyF10VI GCNNNNNNNGC 9 cut(s) 199, 389, 990, 1031, 1081, 1304, 1313, 1781, 2717
Hsp92I GRCGYC 1 cut(s) 1784
HspAI GCGC 4 cut(s) 2624, 2716, 2718, 2809
KpnI GGTACC 2 cut(s) 424, 3411
Ksp22I TGATCA 1 cut(s) 2008
LguI GCTCTTC 1 cut(s) 2925
Lsp1109I GCAGC 4 cut(s) 2365, 2530, 2950, 3085
MabI ACCWGGT 1 cut(s) 3403
MaeI CTAG 7 cut(s) 531, 1077, 1530, 2169, 2315, 2790, 3273
MaeIII GTNAC 4 cut(s) 2364, 2472, 3385, 3399
MfeI CAATTG 1 cut(s) 995
MflI RGATCY 2 cut(s) 662, 1255
MhlI GDGCHC 7 cut(s) 84, 900, 1043, 1438, 2713, 3240, 3294
MlyI GAGTC 1 cut(s) 2121
MmeI TCCRAC 3 cut(s) 1728, 3177, 3205
MroXI GAANNNNTTC 2 cut(s) 616, 1336
MslI CAYNNNNRTG 3 cut(s) 104, 1358, 2676
MspA1I CMGCKG 3 cut(s) 227, 1889, 2356
MspCI CTTAAG 1 cut(s) 2567
MspI CCGG 2 cut(s) 104, 3283
MspR9I CCNGG 7 cut(s) 1456, 2211, 2442, 2777, 2894, 3283, 3405
MssI GTTTAAAC 1 cut(s) 802
MunI CAATTG 1 cut(s) 995
Mva1269I GAATGC 3 cut(s) 70, 1765, 1980
MvaI CCWGG 6 cut(s) 1456, 2211, 2442, 2777, 2894, 3405
MvnI CGCG 2 cut(s) 1098, 2718
MwoI GCNNNNNNNGC 9 cut(s) 199, 389, 990, 1031, 1081, 1304, 1313, 1781, 2717
NciI CCSGG 1 cut(s) 3283
NcoI CCATGG 3 cut(s) 135, 1473, 2145
NdeI CATATG 2 cut(s) 1969, 2118
NheI GCTAGC 1 cut(s) 2314
NlaIV GGNNCC 7 cut(s) 422, 1041, 2891, 2924, 3270, 3316, 3409
NmuCI GTSAC 2 cut(s) 2364, 3385
NsbI TGCGCA 2 cut(s) 2625, 2810
NspI RCATGY 2 cut(s) 2365, 2398
OliI CACNNNNGTG 1 cut(s) 104
PaeI GCATGC 1 cut(s) 2398
PaeR7I CTCGAG 1 cut(s) 3038
PagI TCATGA 3 cut(s) 585, 913, 1018
PaqCI CACCTGC 1 cut(s) 1080
PasI CCCWGGG 2 cut(s) 1455, 2776
PauI GCGCGC 1 cut(s) 2716
PceI AGGCCT 1 cut(s) 392
PciSI GCTCTTC 1 cut(s) 2925
PctI GAATGC 3 cut(s) 70, 1765, 1980
PdmI GAANNNNTTC 2 cut(s) 616, 1336
PfeI GAWTC 7 cut(s) 131, 1832, 2249, 2279, 2606, 2801, 3173
PflMI CCANNNNNTGG 1 cut(s) 230
PinAI ACCGGT 1 cut(s) 103
PkrI GCNGC 7 cut(s) 204, 1876, 2355, 2545, 2683, 2965, 3100
PleI GAGTC 1 cut(s) 2120
PmeI GTTTAAAC 1 cut(s) 802
PpsI GAGTC 1 cut(s) 2120
PpuMI RGGWCCY 1 cut(s) 2890
PshAI GACNNNNGTC 1 cut(s) 1406
PshBI ATTAAT 2 cut(s) 147, 3138
PsiI TTATAA 1 cut(s) 555
Psp124BI GAGCTC 2 cut(s) 84, 1438
Psp5II RGGWCCY 1 cut(s) 2890
Psp6I CCWGG 6 cut(s) 1454, 2209, 2440, 2775, 2892, 3403
PspFI CCCAGC 2 cut(s) 140, 2356
PspGI CCWGG 6 cut(s) 1454, 2209, 2440, 2775, 2892, 3403
PspN4I GGNNCC 7 cut(s) 422, 1041, 2891, 2924, 3270, 3316, 3409
PspOMI GGGCCC 1 cut(s) 1039
PspPI GGNCC 7 cut(s) 1039, 1040, 1243, 1279, 1292, 2694, 2890
PspPPI RGGWCCY 1 cut(s) 2890
PstI CTGCAG 2 cut(s) 270, 2260
PstNI CAGNNNCTG 1 cut(s) 380
PsuI RGATCY 2 cut(s) 662, 1255
PteI GCGCGC 1 cut(s) 2716
PvuII CAGCTG 3 cut(s) 227, 1889, 2356
RsaI GTAC 8 cut(s) 422, 1580, 1666, 2262, 2863, 3023, 3128, 3409
RsaNI GTAC 8 cut(s) 421, 1579, 1665, 2261, 2862, 3022, 3127, 3408
RseI CAYNNNNRTG 3 cut(s) 104, 1358, 2676
SacI GAGCTC 2 cut(s) 84, 1438
SapI GCTCTTC 1 cut(s) 2925
SatI GCNGC 7 cut(s) 203, 1875, 2354, 2544, 2682, 2964, 3099
Sau96I GGNCC 7 cut(s) 1039, 1040, 1243, 1279, 1292, 2694, 2890
ScaI AGTACT 1 cut(s) 1580
SchI GAGTC 1 cut(s) 2121
ScrFI CCNGG 7 cut(s) 1456, 2211, 2442, 2777, 2894, 3283, 3405
SduI GDGCHC 7 cut(s) 84, 900, 1043, 1438, 2713, 3240, 3294
SexAI ACCWGGT 1 cut(s) 3403
SfcI CTRYAG 5 cut(s) 266, 1791, 2256, 2297, 3177
Sfr274I CTCGAG 1 cut(s) 3038
SgrAI CRCCGGYG 1 cut(s) 103
SinI GGWCC 3 cut(s) 1243, 1279, 2890
SlaI CTCGAG 1 cut(s) 3038
SmiMI CAYNNNNRTG 3 cut(s) 104, 1358, 2676
SmlI CTYRAG 4 cut(s) 188, 736, 2567, 3038
SmoI CTYRAG 4 cut(s) 188, 736, 2567, 3038
SphI GCATGC 1 cut(s) 2398
SseBI AGGCCT 1 cut(s) 392
SsiI CCGC 7 cut(s) 203, 746, 826, 1875, 2465, 2681, 3428
SspI AATATT 1 cut(s) 551
SspMI CTAG 7 cut(s) 531, 1077, 1530, 2169, 2315, 2790, 3273
SstI GAGCTC 2 cut(s) 84, 1438
StuI AGGCCT 1 cut(s) 392
StyD4I CCNGG 7 cut(s) 1454, 2209, 2440, 2775, 2892, 3281, 3403
StyI CCWWGG 5 cut(s) 135, 1035, 1076, 1473, 2145
TaqI TCGA 6 cut(s) 8, 84, 610, 834, 3039, 3243
TaqII GACCGA 1 cut(s) 3062
TatI WGTACW 3 cut(s) 1578, 1664, 3021
TauI GCSGC 3 cut(s) 205, 1877, 2684
TfiI GAWTC 7 cut(s) 131, 1832, 2249, 2279, 2606, 2801, 3173
TscAI CASTG 8 cut(s) 525, 1505, 1596, 1964, 2326, 2373, 2467, 2718
TseFI GTSAC 2 cut(s) 2364, 3385
TseI GCWGC 4 cut(s) 2353, 2543, 2963, 3098
Tsp45I GTSAC 2 cut(s) 2364, 3385
TspGWI ACGGA 2 cut(s) 1235, 1271
TspRI CASTG 8 cut(s) 525, 1505, 1596, 1964, 2326, 2373, 2467, 2718
Van91I CCANNNNNTGG 1 cut(s) 230
Vha464I CTTAAG 1 cut(s) 2567
VneI GTGCAC 2 cut(s) 2709, 3236
VpaK11BI GGWCC 3 cut(s) 1243, 1279, 2890
VspI ATTAAT 2 cut(s) 147, 3138
XapI RAATTY 9 cut(s) 306, 581, 753, 874, 1621, 2057, 2064, 2870, 3334
XceI RCATGY 2 cut(s) 2365, 2398
XhoI CTCGAG 1 cut(s) 3038
XmaJI CCTAGG 1 cut(s) 1076
XmiI GTMKAC 1 cut(s) 1824
XmnI GAANNNNTTC 2 cut(s) 616, 1336
XspI CTAG 7 cut(s) 531, 1077, 1530, 2169, 2315, 2790, 3273
ZrmI AGTACT 1 cut(s) 1580
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.