MD13G1257100.v1.1

Autophagy-related protein 11

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0008019
Physical Location & Seq
Forward (+)
28907779 .. 28920814
13036 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1257100.v1.1.491

Sequence Viewer

Length: 3519 bp
ATGAGTTCAAGTACGGCTGAAGGTTTGGTTCATCAGGGCAAGCTTTTGGTTCATATTGCTGAGAATGGGCATTCATTTGAGCTTGATTGTGAAGGAACCACACTGGTAGAGGTAGTTATGCGGTATATTGAATCTATGGCTGGGATTAATCTCAATGACCAGCTTGTTCTGTGTTTGGATATGAAACTTACGCCACAGAGGCCGCTTTCAGCTTATAACCTTCCAGCTGATGGTCGGGAAGTGTTTATATTCAATAAAGCAAGGCTGCAAACTAATTCTTCGCTCCCTCCGCCTGAGCAAGTTGATATTCTTGACACTGCTGACCCCCAGTCACCGTCAGCTTCACATGACCCTCACCCTTTGGATGATGCTTTGGACCCTGCTTTGAAGGCCTTGCCTTCTTATGAAAGGCAATTTAGGTACCACTACCACCAGGGTCATGCAATTTACAGTCGTACCCAAGTAAAATATGAGAACTGTGAGAGGCTTTTGATGGAGCAGAAGGTTCAAGAGTGGGCAGTGGAGGTTGCTAGGGGTAATTTGGATCAATATTATAAAATGATAAACCAAACCTATACAGAATTCATGAAGCGTTATTCCCAACAGCATCGTAGTCATTCTGATCTTTTGATCAATCTCTGGAGGGATGTAGAAAAACTGAGATCTGTCAAGCTTCACCCTGCATTAGAGACTGCCACTTGGAAGTGCCTATCGGATTTTGTGAAGGAAGAGACCTTGAGGAAAGCGGGAGAAATTTGTAGCAGTTCCCACAGGCAGTTTGAGAATAAGGTTTCACAGTTTAAGCAGATGTTTAGTGAGGTAAAGCAGAAAGTCGAAGAATTGTTTTCTAACAGGGCTTCACTGCCCATTAGGCATTTGGAACTCACAATTAAGGATCACCAAAGATATATTCATGAGCAAAAGAGTATAATGCAATCTTTGAGCAAAGATGTAAATACTGTCAAGAAAGTCGTGGATGATTGCTTGTCTTGCCAATTGTCATCCTCACTTTGTCCACATGATGCAGTTTCAGCCTTGGGCCCTATGTATGATGTCCATGATAAAAATCACCTGCCTAGGATGCAGGCTTGTGATCATGCAATTTCTAAGCTCCTCGACTTCTGCAAGGATAAAAAGAATGAAATGAACGTATTTCTCCATAATTATATGCAAAAGATAGCATATATTTCTTACATAATTAAAGATGCAAAATTACAATTTCCTGTTTTTAGAGAGGCAATGGTGCGTCAAGAGGATCTATTTTTGGACTTAAAACTGGTTCGTGGGATTGACCCTGCATATAGAGCCTGCTTAGCAGAAATAGTCAGACGAAAGGCTTCCTTGAAGCTTTACATGGGGATGGCTGGACAGTTGGCTGAAAGACTTGCGACAAAAAGGGAGACTGAAGTCAGGAGACGGGAGGAGTTTTTGAAAGATCACAGTTTATACATACCCAGGGATGTATTAGCATCCATGGGATTATATGATACCCTCAATCAGTGTGATGTCAACATAGCTCCTTTTGATACTAGCTTGCTTGATATTGAGATTTCAGACATTGACCATTATGCTCCTGAGTACTTGACTCCACTGTCTTCCAAGAGTGCGTTTAGAGGTTCATATTCTATGTCTAACGAAACTTGTCATTCGGTTGGTTCTGACGAGAGTACATTGGATAACCTTGAGAATTGTGACTCTGAGGAGCTACTTGAAGGCGGTGAGTTGGTAGAGATTGCTGGAACAGGTAAGTTAGAAGTTGAGAATGCAAAATTGAAAGCAGAACTGGCTTCTGCAATAGCTGTGATCTGTTCATTCTGGCCTGAAGTAGACTTTGAATCATTGGATGATAGTAAAGTGGATAATTTATTGAAAAGTGCCGCAGAGAAGACAACTGAAGCCTTGCACCTGAAAGATGAATATGGAAAGCATCTACAATCTATGCTTAGGGTGAAGCAGATGCAGTGTCTTTCATATGAGAAACGCATTCAAGAACTTGAGCAGAGACTGTCTGATCAGTATTTGCAAAGCCAGAAGATTTCAAATGATAAGGATGCCTCTGAATTGTCTGATAAGGTTGATGACTGCAAGCAAGAAATCATAGGTGGTAGGGAAGTCCATATGCCTTGCTCATCTAATACTGAGCCCATGGATGAGGTTTCCTGTGTTTCTAGTGTTTTGGATGCAAAATTGGGTCTGTTCAATGTGCAACCTGGAAAAATGAAAGATGGGGTAGATAAGAATATGATGGATTCTTCGGCAGTACGAAATCATCTAATGGATTCATCCATGCAAAATCATCCACTGGATTCATCCATGCAAGAGCTACAGCGTGAAGAACTGCTGGCCAGTGGTAAAGATGGGAAAGAAAAGATGGTGGGGCAGCTGGGCATGTCACTGACAAACAGTTCTACTGCTGAAAGCATGCCTGAACCTCTTAATGTTATACCGTGTGAAACAGCAATTGACCCAGGCTTGAACACCAAAGTCAGTGCGGAGCTGTTGTTGGAATTGCAAACCGCACTTTCAGAGAAGTCAAACCAGTTGAGTGAAACAGAAATTAAGCTTAAAGCTACTATGGAGGATGTTTCTATACTTAAGAGGGAATTGGACACAAATCGGAAACTCCTTGATGAATCTCAGATGAATTGTGCTCACTTGGAGAATTGTTTGCACGAGGCAAGAGAGGAAGCTCAAACCCATCTTTGTGCGGCTGATCGCAGGGCCTCGGAGTATAGTGCGCTGCGCACATCTGCTGTGAAAATGCGTGGCATCTTCGAAAGATTAAGGAGCTGTGTATATGCCCAGGGTGGGGTAGCTAGTTTTAATGAATCTTTGCACACCTTGGCACAATCTCTGGGCAATTCTATTAACGGCAGTGAAGATGATGGTACTGTTGAATTTCGGAAATGTATTCGGGCTCTGGCAGATAGAGTTGGTTTCTTGTCAAGGCACCGTGAAGAGCTGCTTGACAAGTATCCAAAGGTTGAAGCTGTAAATGAACAACTTAGAAAGGATTTGGAAGAGAAGAAGGGGCTGGTTAAAACTTTGTACACAAAGCATCAACTTGAGAAGCAGGCAAACAAAGAGAAGATATCTTTCAGCCGCTTGGAAGTCCACGAGATTGCTGCATTTGTTCTAAACACATATGGGCAGTACGAGGCAATTAGTCGTAATTGCTCCAACTACTACCTGTCTGCTGAATCTGTAGCCTTGTTTACTGATCATCTCCCAAATCAACCTAACTACATTGTTGGGCAAATTGTGCATGTCGAACGGCAGACCGTGAAGCCATTGGCTCCTAGTTCAACGCGGTCTGAGAACAACCTAACATCTGACATGGAAACCGACCGGTTGACCTTGAATTCAGGATTGAACCCGTATGGTCTCCCTATTGGCTGTGAATACTTCGTAGTGACAGTAGCCATGTTACCTGTACCACCACCATTCATACCCCACCTCCTTCCTGATCCCTGCACTAGGCAGATTGACGTGATGGAAGAATCCCAAGTGTACATATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000045 GO:0000272 GO:0000407 GO:0000422 GO:0001932 GO:0001934 GO:0003674 GO:0005198 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005776 GO:0005911 GO:0005975 GO:0005976 GO:0005977 GO:0005980 GO:0006073 GO:0006091 GO:0006112 GO:0006914 GO:0006996 GO:0007005 GO:0007033 GO:0007275 GO:0007568 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009251 GO:0009506 GO:0009893 GO:0009987 GO:0010150 GO:0010562 GO:0010604 GO:0015980 GO:0016020 GO:0016043 GO:0016052 GO:0016236 GO:0019220 GO:0019222 GO:0019899 GO:0019900 GO:0019901 GO:0022411 GO:0022607 GO:0030054 GO:0030242 GO:0031323 GO:0031325 GO:0031399 GO:0031401 GO:0032268 GO:0032270 GO:0032501 GO:0032502 GO:0032947 GO:0032991 GO:0034045 GO:0042325 GO:0042327 GO:0042802 GO:0042803 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044042 GO:0044085 GO:0044237 GO:0044238 GO:0044247 GO:0044248 GO:0044260 GO:0044262 GO:0044264 GO:0044275 GO:0044424 GO:0044444 GO:0044464 GO:0045937 GO:0046983 GO:0048366 GO:0048367 GO:0048518 GO:0048522 GO:0048731 GO:0048827 GO:0048856 GO:0050789 GO:0050794 GO:0051171 GO:0051173 GO:0051174 GO:0051246 GO:0051247 GO:0055044 GO:0055114 GO:0060255 GO:0061695 GO:0061709 GO:0061723 GO:0061726 GO:0061912 GO:0061919 GO:0065007 GO:0070925 GO:0071704 GO:0071840 GO:0080090 GO:0090693 GO:0098805 GO:0099402 GO:1901575 GO:1902494 GO:1902554 GO:1902911 GO:1903008 GO:1905037 GO:1990234 GO:1990316
KEGG Pathways
Metabolic & Signaling
ko04136 ko04138 ko04139 map04136 map04138 map04139
Pfam Domains
Protein Families
APG17 ATG11

Protein Analysis

1173

Amino Acids

132.78

Weight (kDa)

5.44

Isoelectric Point (pI)

43.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ATG17_like PF04108 147 - 483 6.9e-31 Autophagy protein ATG17-like domain
ATG11 PF10377 1003 - 1142 1.1e-18 Autophagy-related protein 11
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 216, 555
AarI CACCTGC 1 cut(s) 1080
AasI GACNNNNNNGTC 1 cut(s) 1591
Acc16I TGCGCA 1 cut(s) 2744
Acc36I ACCTGC 1 cut(s) 1080
Acc65I GGTACC 1 cut(s) 420
AccB1I GGYRCC 2 cut(s) 420, 2949
AccB7I CCANNNNNTGG 1 cut(s) 230
AccI GTMKAC 1 cut(s) 1827
AccII CGCG 1 cut(s) 3310
AclWI GGATC 4 cut(s) 552, 903, 1263, 3461
AcoI YGGCCR 1 cut(s) 2343
AcsI RAATTY 4 cut(s) 581, 753, 2897, 3361
AcuI CTGAAG 4 cut(s) 39, 1425, 1842, 1914
AfiI CCNNNNNNNGG 4 cut(s) 230, 2808, 2809, 3477
AflII CTTAAG 1 cut(s) 2594
AgeI ACCGGT 1 cut(s) 3348
AhdI GACNNNNNGTC 1 cut(s) 328
AjiI CACGTC 1 cut(s) 3490
AjnI CCWGG 5 cut(s) 432, 1454, 2209, 2467, 2802
AjuI GAANNNNNNNTTGG 2 cut(s) 894, 926
Alw21I GWGCWC 1 cut(s) 2653
Alw26I GTCTC 6 cut(s) 683, 725, 1394, 1408, 1996, 3389
AlwI GGATC 4 cut(s) 552, 903, 1263, 3461
AlwNI CAGNNNCTG 1 cut(s) 2005
AoxI GGCC 6 cut(s) 200, 390, 1039, 1817, 2343, 2721
ApaI GGGCCC 1 cut(s) 1043
ApeKI GCWGC 5 cut(s) 265, 2380, 2740, 2962, 3125
ApoI RAATTY 4 cut(s) 581, 753, 2897, 3361
AseI ATTAAT 1 cut(s) 147
AsiGI ACCGGT 1 cut(s) 3348
Asp700I GAANNNNTTC 1 cut(s) 1336
Asp718I GGTACC 1 cut(s) 420
AspA2I CCTAGG 1 cut(s) 1076
AspLEI GCGC 2 cut(s) 2740, 2745
AspS9I GGNCC 4 cut(s) 376, 1039, 1040, 2721
AsuHPI GGTGA 7 cut(s) 324, 347, 668, 890, 1061, 1730, 1960
AsuII TTCGAA 1 cut(s) 2775
AvaII GGWCC 1 cut(s) 376
AvrII CCTAGG 1 cut(s) 1076
BaeGI GKGCMC 1 cut(s) 1043
BaeI ACNNNNGTAYC 2 cut(s) 439, 472
BalI TGGCCA 1 cut(s) 2345
BanI GGYRCC 2 cut(s) 420, 2949
BanII GRGCYC 3 cut(s) 1043, 2145, 2920
BauI CACGAG 2 cut(s) 2672, 3116
BbsI GAAGAC 2 cut(s) 1587, 1892
Bbv12I GWGCWC 1 cut(s) 2653
BbvI GCAGC 5 cut(s) 252, 2392, 2727, 2949, 3112
BceAI ACGGC 3 cut(s) 30, 2887, 3290
BcgI CGANNNNNNTGC 2 cut(s) 3107, 3141
BciT130I CCWGG 5 cut(s) 434, 1456, 2211, 2469, 2804
BciVI GTATCC 1 cut(s) 2985
BclI TGATCA 4 cut(s) 630, 1093, 2011, 3220
BcoDI GTCTC 6 cut(s) 683, 725, 1394, 1408, 1996, 3389
BfaI CTAG 7 cut(s) 531, 1077, 1530, 2169, 2817, 3300, 3477
BfmI CTRYAG 2 cut(s) 2324, 3204
BfrI CTTAAG 1 cut(s) 2594
BfuAI ACCTGC 1 cut(s) 1080
BfuI GTATCC 1 cut(s) 2985
BglI GCCNNNNNGGC 2 cut(s) 199, 871
BglII AGATCT 1 cut(s) 662
BisI GCNGC 9 cut(s) 203, 266, 1878, 2381, 2709, 2741, 2963, 3103, 3126
BlnI CCTAGG 1 cut(s) 1076
BlpI GCTNAGC 1 cut(s) 1312
BlsI GCNGC 9 cut(s) 204, 267, 1879, 2382, 2710, 2742, 2964, 3104, 3127
BmcAI AGTACT 1 cut(s) 1580
Bme1390I CCNGG 5 cut(s) 434, 1456, 2211, 2469, 2804
Bme18I GGWCC 1 cut(s) 376
BmeRI GACNNNNNGTC 1 cut(s) 328
BmgBI CACGTC 1 cut(s) 3490
BmgT120I GGNCC 4 cut(s) 376, 1039, 1040, 2721
BmiI GGNNCC 6 cut(s) 97, 378, 422, 1041, 2951, 3297
BmrFI CCNGG 5 cut(s) 434, 1456, 2211, 2469, 2804
BmrI ACTGGG 1 cut(s) 322
BmuI ACTGGG 1 cut(s) 322
BoxI GACNNNNGTC 1 cut(s) 1406
BpiI GAAGAC 2 cut(s) 1587, 1892
BpmI CTGGAG 1 cut(s) 661
Bpu10I CCTNAGC 2 cut(s) 294, 1943
Bpu1102I GCTNAGC 1 cut(s) 1312
Bpu14I TTCGAA 1 cut(s) 2775
BpuEI CTTGAG 4 cut(s) 757, 1703, 2015, 3086
BsaBI GATNNNNATC 1 cut(s) 1065
BsaI GGTCTC 2 cut(s) 725, 3389
BsaWI WCCGGW 1 cut(s) 3348
BsaXI ACNNNNNCTCC 4 cut(s) 2486, 2516, 3441, 3471
Bsc4I CCNNNNNNNGG 4 cut(s) 230, 2808, 2809, 3477
Bse118I RCCGGY 1 cut(s) 3348
Bse1I ACTGG 7 cut(s) 108, 328, 1281, 1788, 2307, 2346, 2539
Bse3DI GCAATG 1 cut(s) 1245
Bse8I GATNNNNATC 1 cut(s) 1065
BseBI CCWGG 5 cut(s) 434, 1456, 2211, 2469, 2804
BseJI GATNNNNATC 1 cut(s) 1065
BseLI CCNNNNNNNGG 4 cut(s) 230, 2808, 2809, 3477
BseMI GCAATG 1 cut(s) 1245
BseMII CTCAG 8 cut(s) 51, 285, 650, 1566, 1689, 2130, 2651, 3306
BseNI ACTGG 7 cut(s) 108, 328, 1281, 1788, 2307, 2346, 2539
BseRI GAGGAG 3 cut(s) 1103, 1436, 1715
BseSI GKGCMC 1 cut(s) 1043
BseXI GCAGC 5 cut(s) 252, 2392, 2727, 2949, 3112
BseYI CCCAGC 2 cut(s) 140, 2383
BsgI GTGCAG 1 cut(s) 3457
Bsh1236I CGCG 1 cut(s) 3310
Bsh1285I CGRYCG 1 cut(s) 3349
BshFI GGCC 6 cut(s) 202, 392, 1041, 1819, 2345, 2723
BshNI GGYRCC 2 cut(s) 420, 2949
BshTI ACCGGT 1 cut(s) 3348
BsiEI CGRYCG 1 cut(s) 3349
BsiHKAI GWGCWC 1 cut(s) 2653
BsiSI CCGG 1 cut(s) 3349
BslI CCNNNNNNNGG 4 cut(s) 230, 2808, 2809, 3477
BsmAI GTCTC 6 cut(s) 683, 725, 1394, 1408, 1996, 3389
BsmBI CGTCTC 1 cut(s) 1408
BsmI GAATGC 3 cut(s) 70, 1768, 1983
BsnI GGCC 6 cut(s) 202, 392, 1041, 1819, 2345, 2723
Bso31I GGTCTC 2 cut(s) 725, 3389
Bsp119I TTCGAA 1 cut(s) 2775
Bsp120I GGGCCC 1 cut(s) 1039
Bsp1286I GDGCHC 4 cut(s) 1043, 2145, 2653, 2920
Bsp1407I TGTACA 2 cut(s) 3048, 3510
Bsp1720I GCTNAGC 1 cut(s) 1312
Bsp19I CCATGG 2 cut(s) 1473, 2145
BspANI GGCC 6 cut(s) 202, 392, 1041, 1819, 2345, 2723
BspCNI CTCAG 8 cut(s) 52, 286, 651, 1567, 1690, 2131, 2650, 3307
BspFNI CGCG 1 cut(s) 3310
BspHI TCATGA 2 cut(s) 585, 913
BspLI GGNNCC 6 cut(s) 97, 378, 422, 1041, 2951, 3297
BspMI ACCTGC 1 cut(s) 1080
BspPI GGATC 4 cut(s) 552, 903, 1263, 3461
BspQI GCTCTTC 1 cut(s) 2952
BspT104I TTCGAA 1 cut(s) 2775
BspT107I GGYRCC 2 cut(s) 420, 2949
BspTI CTTAAG 1 cut(s) 2594
BspTNI GGTCTC 2 cut(s) 725, 3389
BsrDI GCAATG 1 cut(s) 1245
BsrFI RCCGGY 1 cut(s) 3348
BsrGI TGTACA 2 cut(s) 3048, 3510
BsrI ACTGG 7 cut(s) 108, 328, 1281, 1788, 2307, 2346, 2539
BssAI RCCGGY 1 cut(s) 3348
BssSI CACGAG 2 cut(s) 2672, 3116
BssT1I CCWWGG 5 cut(s) 1035, 1076, 1473, 2145, 2841
Bst2BI CACGAG 2 cut(s) 2672, 3116
Bst2UI CCWGG 5 cut(s) 434, 1456, 2211, 2469, 2804
Bst6I CTCTTC 3 cut(s) 723, 2952, 3015
BstAFI CTTAAG 1 cut(s) 2594
BstAPI GCANNNNNTGC 1 cut(s) 3262
BstAUI TGTACA 2 cut(s) 3048, 3510
BstBI TTCGAA 1 cut(s) 2775
BstC8I GCNNGC 8 cut(s) 41, 1086, 1309, 1535, 2087, 2343, 2423, 3075
BstDSI CCRYGG 2 cut(s) 1473, 2145
BstENI CCTNNNNNAGG 1 cut(s) 3475
BstFNI CGCG 1 cut(s) 3310
BstHHI GCGC 2 cut(s) 2740, 2745
BstMAI GTCTC 6 cut(s) 683, 725, 1394, 1408, 1996, 3389
BstMCI CGRYCG 1 cut(s) 3349
BstNI CCWGG 5 cut(s) 434, 1456, 2211, 2469, 2804
BstNSI RCATGY 3 cut(s) 2392, 2425, 3269
BstPAI GACNNNNGTC 1 cut(s) 1406
BstSCI CCNGG 5 cut(s) 432, 1454, 2209, 2467, 2802
BstSFI CTRYAG 2 cut(s) 2324, 3204
BstSLI GKGCMC 1 cut(s) 1043
BstUI CGCG 1 cut(s) 3310
BstV1I GCAGC 5 cut(s) 252, 2392, 2727, 2949, 3112
BstV2I GAAGAC 2 cut(s) 1587, 1892
BstX2I RGATCY 2 cut(s) 662, 1255
BstYI RGATCY 2 cut(s) 662, 1255
BsuI GTATCC 1 cut(s) 2985
BsuRI GGCC 6 cut(s) 202, 392, 1041, 1819, 2345, 2723
BtgI CCRYGG 2 cut(s) 1473, 2145
BtrI CACGTC 1 cut(s) 3490
BtsI GCAGTG 5 cut(s) 315, 525, 860, 1967, 2881
BveI ACCTGC 1 cut(s) 1080
Cac8I GCNNGC 8 cut(s) 41, 1086, 1309, 1535, 2087, 2343, 2423, 3075
CaiI CAGNNNCTG 1 cut(s) 2005
CciI TCATGA 2 cut(s) 585, 913
CfoI GCGC 2 cut(s) 2740, 2745
Cfr10I RCCGGY 1 cut(s) 3348
Cfr13I GGNCC 4 cut(s) 376, 1039, 1040, 2721
CseI GACGC 1 cut(s) 1235
CspAI ACCGGT 1 cut(s) 3348
DrdI GACNNNNNNGTC 1 cut(s) 1591
DriI GACNNNNNGTC 1 cut(s) 328
DseDI GACNNNNNNGTC 1 cut(s) 1591
EaeI YGGCCR 1 cut(s) 2343
Eam1104I CTCTTC 3 cut(s) 723, 2952, 3015
Eam1105I GACNNNNNGTC 1 cut(s) 328
EarI CTCTTC 3 cut(s) 723, 2952, 3015
EciI GGCGGA 1 cut(s) 279
Eco130I CCWWGG 5 cut(s) 1035, 1076, 1473, 2145, 2841
Eco147I AGGCCT 1 cut(s) 392
Eco24I GRGCYC 3 cut(s) 1043, 2145, 2920
Eco31I GGTCTC 2 cut(s) 725, 3389
Eco32I GATATC 1 cut(s) 3093
Eco47I GGWCC 1 cut(s) 376
Eco57I CTGAAG 4 cut(s) 39, 1425, 1842, 1914
EcoNI CCTNNNNNAGG 1 cut(s) 3475
EcoO109I RGGNCCY 2 cut(s) 1040, 2721
EcoRI GAATTC 2 cut(s) 581, 3361
EcoRII CCWGG 5 cut(s) 432, 1454, 2209, 2467, 2802
EcoRV GATATC 1 cut(s) 3093
EcoT14I CCWWGG 5 cut(s) 1035, 1076, 1473, 2145, 2841
EcoT38I GRGCYC 3 cut(s) 1043, 2145, 2920
ErhI CCWWGG 5 cut(s) 1035, 1076, 1473, 2145, 2841
Esp3I CGTCTC 1 cut(s) 1408
FalI AAGNNNNNCTT 4 cut(s) 1325, 1357, 2949, 2981
FauI CCCGC 1 cut(s) 739
FauNDI CATATG 4 cut(s) 1972, 2118, 3145, 3515
FbaI TGATCA 4 cut(s) 630, 1093, 2011, 3220
FblI GTMKAC 1 cut(s) 1827
Fnu4HI GCNGC 9 cut(s) 203, 266, 1878, 2381, 2709, 2741, 2963, 3103, 3126
FriOI GRGCYC 3 cut(s) 1043, 2145, 2920
Fsp4HI GCNGC 9 cut(s) 203, 266, 1878, 2381, 2709, 2741, 2963, 3103, 3126
FspBI CTAG 7 cut(s) 531, 1077, 1530, 2169, 2817, 3300, 3477
FspI TGCGCA 1 cut(s) 2744
GlaI GCGC 2 cut(s) 2739, 2744
GluI GCNGC 9 cut(s) 203, 266, 1878, 2381, 2709, 2741, 2963, 3103, 3126
GsaI CCCAGC 2 cut(s) 144, 2387
GsuI CTGGAG 1 cut(s) 661
HaeIII GGCC 6 cut(s) 202, 392, 1041, 1819, 2345, 2723
HapII CCGG 1 cut(s) 3349
HgaI GACGC 1 cut(s) 1235
HhaI GCGC 2 cut(s) 2740, 2745
Hin6I GCGC 2 cut(s) 2738, 2743
HinP1I GCGC 2 cut(s) 2738, 2743
HincII GTYRAC 2 cut(s) 1510, 3354
HindII GTYRAC 2 cut(s) 1510, 3354
HindIII AAGCTT 4 cut(s) 41, 671, 1346, 2561
HpaII CCGG 1 cut(s) 3349
HphI GGTGA 7 cut(s) 324, 347, 668, 890, 1061, 1730, 1960
Hpy166II GTNNAC 8 cut(s) 1016, 1510, 1828, 3051, 3115, 3216, 3354, 3511
Hpy8I GTNNAC 8 cut(s) 1016, 1510, 1828, 3051, 3115, 3216, 3354, 3511
HpyCH4IV ACGT 2 cut(s) 1149, 3489
HpySE526I ACGT 2 cut(s) 1149, 3489
HspAI GCGC 2 cut(s) 2738, 2743
KpnI GGTACC 1 cut(s) 424
Ksp22I TGATCA 4 cut(s) 630, 1093, 2011, 3220
LguI GCTCTTC 1 cut(s) 2952
Lsp1109I GCAGC 5 cut(s) 252, 2392, 2727, 2949, 3112
MaeI CTAG 7 cut(s) 531, 1077, 1530, 2169, 2817, 3300, 3477
MaeII ACGT 2 cut(s) 1149, 3489
MaeIII GTNAC 5 cut(s) 330, 1691, 2391, 3412, 3426
MfeI CAATTG 2 cut(s) 995, 2460
MflI RGATCY 2 cut(s) 662, 1255
MhlI GDGCHC 4 cut(s) 1043, 2145, 2653, 2920
MlsI TGGCCA 1 cut(s) 2345
MluNI TGGCCA 1 cut(s) 2345
MlyI GAGTC 2 cut(s) 1579, 1688
MmeI TCCRAC 2 cut(s) 2484, 3204
Mox20I TGGCCA 1 cut(s) 2345
MroXI GAANNNNTTC 1 cut(s) 1336
MscI TGGCCA 1 cut(s) 2345
MslI CAYNNNNRTG 2 cut(s) 1358, 2703
Msp20I TGGCCA 1 cut(s) 2345
MspA1I CMGCKG 2 cut(s) 227, 2383
MspCI CTTAAG 1 cut(s) 2594
MspI CCGG 1 cut(s) 3349
MspR9I CCNGG 5 cut(s) 434, 1456, 2211, 2469, 2804
MteI GCGCNGCGC 1 cut(s) 2741
MunI CAATTG 2 cut(s) 995, 2460
Mva1269I GAATGC 3 cut(s) 70, 1768, 1983
MvaI CCWGG 5 cut(s) 434, 1456, 2211, 2469, 2804
MvnI CGCG 1 cut(s) 3310
NcoI CCATGG 2 cut(s) 1473, 2145
NdeI CATATG 4 cut(s) 1972, 2118, 3145, 3515
NlaIV GGNNCC 6 cut(s) 97, 378, 422, 1041, 2951, 3297
NmuCI GTSAC 4 cut(s) 330, 1691, 2391, 3412
NsbI TGCGCA 1 cut(s) 2744
NspI RCATGY 3 cut(s) 2392, 2425, 3269
NspV TTCGAA 1 cut(s) 2775
PaeI GCATGC 1 cut(s) 2425
PagI TCATGA 2 cut(s) 585, 913
PaqCI CACCTGC 1 cut(s) 1080
PasI CCCWGGG 2 cut(s) 1455, 2803
PceI AGGCCT 1 cut(s) 392
PciSI GCTCTTC 1 cut(s) 2952
PctI GAATGC 3 cut(s) 70, 1768, 1983
PdmI GAANNNNTTC 1 cut(s) 1336
PfeI GAWTC 9 cut(s) 131, 1835, 2249, 2279, 2306, 2633, 2828, 3200, 3500
PflMI CCANNNNNTGG 1 cut(s) 230
PinAI ACCGGT 1 cut(s) 3348
PkrI GCNGC 9 cut(s) 204, 267, 1879, 2382, 2710, 2742, 2964, 3104, 3127
PleI GAGTC 2 cut(s) 1579, 1688
PpsI GAGTC 2 cut(s) 1579, 1688
PshAI GACNNNNGTC 1 cut(s) 1406
PshBI ATTAAT 1 cut(s) 147
PsiI TTATAA 2 cut(s) 216, 555
Psp6I CCWGG 5 cut(s) 432, 1454, 2209, 2467, 2802
PspFI CCCAGC 2 cut(s) 140, 2383
PspGI CCWGG 5 cut(s) 432, 1454, 2209, 2467, 2802
PspN4I GGNNCC 6 cut(s) 97, 378, 422, 1041, 2951, 3297
PspOMI GGGCCC 1 cut(s) 1039
PspPI GGNCC 4 cut(s) 376, 1039, 1040, 2721
PstNI CAGNNNCTG 1 cut(s) 2005
PsuI RGATCY 2 cut(s) 662, 1255
PvuII CAGCTG 2 cut(s) 227, 2383
RseI CAYNNNNRTG 2 cut(s) 1358, 2703
SapI GCTCTTC 1 cut(s) 2952
SatI GCNGC 9 cut(s) 203, 266, 1878, 2381, 2709, 2741, 2963, 3103, 3126
Sau96I GGNCC 4 cut(s) 376, 1039, 1040, 2721
ScaI AGTACT 1 cut(s) 1580
SchI GAGTC 2 cut(s) 1579, 1688
ScrFI CCNGG 5 cut(s) 434, 1456, 2211, 2469, 2804
SduI GDGCHC 4 cut(s) 1043, 2145, 2653, 2920
SfcI CTRYAG 2 cut(s) 2324, 3204
SfuI TTCGAA 1 cut(s) 2775
SinI GGWCC 1 cut(s) 376
SmiMI CAYNNNNRTG 2 cut(s) 1358, 2703
SmlI CTYRAG 5 cut(s) 736, 1682, 1994, 2594, 3065
SmoI CTYRAG 5 cut(s) 736, 1682, 1994, 2594, 3065
SphI GCATGC 1 cut(s) 2425
SseBI AGGCCT 1 cut(s) 392
SspI AATATT 1 cut(s) 551
SspMI CTAG 7 cut(s) 531, 1077, 1530, 2169, 2817, 3300, 3477
StuI AGGCCT 1 cut(s) 392
StyD4I CCNGG 5 cut(s) 432, 1454, 2209, 2467, 2802
StyI CCWWGG 5 cut(s) 1035, 1076, 1473, 2145, 2841
TaiI ACGT 2 cut(s) 1152, 3492
TaqI TCGA 4 cut(s) 834, 1116, 2775, 3270
TatI WGTACW 4 cut(s) 1578, 1667, 3048, 3510
TauI GCSGC 4 cut(s) 205, 1880, 2711, 3105
TfiI GAWTC 9 cut(s) 131, 1835, 2249, 2279, 2306, 2633, 2828, 3200, 3500
TseFI GTSAC 4 cut(s) 330, 1691, 2391, 3412
TseI GCWGC 5 cut(s) 265, 2380, 2740, 2962, 3125
Tsp45I GTSAC 4 cut(s) 330, 1691, 2391, 3412
Van91I CCANNNNNTGG 1 cut(s) 230
Vha464I CTTAAG 1 cut(s) 2594
VpaK11BI GGWCC 1 cut(s) 376
VspI ATTAAT 1 cut(s) 147
XagI CCTNNNNNAGG 1 cut(s) 3475
XapI RAATTY 4 cut(s) 581, 753, 2897, 3361
XceI RCATGY 3 cut(s) 2392, 2425, 3269
XcmI CCANNNNNNNNNTGG 1 cut(s) 874
XmaJI CCTAGG 1 cut(s) 1076
XmiI GTMKAC 1 cut(s) 1827
XmnI GAANNNNTTC 1 cut(s) 1336
XspI CTAG 7 cut(s) 531, 1077, 1530, 2169, 2817, 3300, 3477
ZrmI AGTACT 1 cut(s) 1580
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.