Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr04

Orthogroup ID

OG0013516

Physical Location & Seq

Genomic Coordinates Forward (+)

7152989 .. 7155974

Gene Structure 2986 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD04G1057400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 930 bp

ATGACGGGCGGTTTAAGAGTCCTAAGTCGGTCACTGAAACATTACAAACCCACAACCTCTATATTTCCAGAGAATTCACTCTCTCCTCAATCCAACCAATGGATTTACCAAACTCGCCGGACTCTGATTTTAGAATCAGCACCGTCACGCTCCGTCACCCTCCACCGACTCCCTGACTCCGATTCCGGGATTGTTGAGGTTAGGTTGGAGAGGCCGGAGGTGAGAAATGCAATTGGGAAAGATATGCTGAGAGGTTTACAGAACACCTTTGAAGCTGTCAGTGAGGATTCTTCTGCTAATGTTTTGCTGATCCGCAGCAAAGTTCCCAAGGTGTTCTGTGCTGGAGCAGATTTAAAGGAACGGAAAATGATGAGTGCATCTGAAGTTCGAGATTTTGTCAACACACTGCGTGCCACGTTCTCATTTTTAGAGGTACTTTCTATTCCAACAATTGCTGTCATTGAAGGAGCAGCATTGGGTGGTGGACTTGAAATGGCTTTATCATGCGATCTTCGGATATGTGAAGATGCAGTGATGGGCTTGCCAGAAACAGGACTTGCTATCATTCCTGGGGCAGGTGGAACACAGCGGCTTCCTAGATTGGTTGGAAAAGCAATATCAAAGGAACTCATATTCACTGGTAGGAAGATTGGCGGCAGGGAAGCAGTGTCGATAGGTTTGGTAAATTACTGTGTTCCTGCTGGTGAAGCTCATCTAAAGGCACTTGAAGTTGCTCGCAATATAAATGAGAAGGGTCCAATAGCAATAAGGATGGCAAAAAAAGCTATTGATGAGGGACTGGAGGTAGATAAGACCTCAGCTTTGGCTCTTGAAGAACATTGCTATGAAAAGACGTTGAACACAAATGATCGCTTGGAAGCCCTATCAGCATTTGCTGAGAAGCGAAAGCCAAAGTACACTGGAGATTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004300 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0006082 GO:0006629 GO:0006631 GO:0006635 GO:0008150 GO:0008152 GO:0009056 GO:0009062 GO:0009987 GO:0016042 GO:0016054 GO:0016829 GO:0016835 GO:0016836 GO:0019395 GO:0019752 GO:0030258 GO:0032787 GO:0034440 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0044237 GO:0044238 GO:0044242 GO:0044248 GO:0044255 GO:0044281 GO:0044282 GO:0044424 GO:0044444 GO:0044464 GO:0046395 GO:0055114 GO:0071704 GO:0072329 GO:1901575

KEGG Pathways

Metabolic & Signaling

ko00280 ko01100 map00280 map01100

Pfam Domains

Protein Families

ECH_1

Protein Analysis

310

Amino Acids

33.65

Weight (kDa)

8.74

Isoelectric Point (pI)

41.19

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
ECH_1	PF00378	61 - 308	9.5e-59	Enoyl-CoA hydratase/isomerase
ECH_2	PF16113	67 - 236	2e-29	Enoyl-CoA hydratase/isomerase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Publication-ready

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Orthologous Genes (Group: OG0013516)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G16800
fragaria_vesca	FvH4_5g19890
malus_domestica	MD04G1057400.v1.1
prunus_persica	Prupe.5G061300_v2.0.a1 Prupe.5G061300_v2.0.a1
pyrus_communis	pycom04g05100
rosa_chinensis	RchiOBHm_Chr7g0204691
rosa_laevigata	RLG00000003437
rosa_multiflora	Rmu_sc0003221.1_g000066 Rmu_sc0007104.1_g000007
rosa_roxburghii	Rroxscaffold_3G00252860
rosa_rugosa	Rorug07G0085700 Rorug07G0085800
rosa_samantha	Rh7AG215600 Rh7BG212800 Rh7CG228700 Rh7DG223300
rosa_wichuraiana	Rw7G018690

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	566
Acc36I	ACCTGC	1 cut(s)	566
AccB7I	CCANNNNNTGG	1 cut(s)	99
AciI	CCGC	4 cut(s)	9, 313, 589, 654
AclWI	GGATC	1 cut(s)	304
AcsI	RAATTY	1 cut(s)	73
AcuI	CTGAAG	1 cut(s)	402
AdeI	CACNNNGTG	1 cut(s)	410
AfaI	GTAC	2 cut(s)	435, 917
AfiI	CCNNNNNNNGG	4 cut(s)	99, 186, 551, 575
AgsI	TTSAA	6 cut(s)	272, 464, 491, 728, 833, 859
AjnI	CCWGG	1 cut(s)	568
AluBI	AGCT	4 cut(s)	275, 710, 785, 821
AluI	AGCT	4 cut(s)	275, 710, 785, 821
AlwI	GGATC	1 cut(s)	304
AoxI	GGCC	1 cut(s)	212
ApeKI	GCWGC	2 cut(s)	315, 470
ApoI	RAATTY	1 cut(s)	73
ArsI	GACNNNNNNTTYG	2 cut(s)	805, 837
AspS9I	GGNCC	1 cut(s)	755
AsuC2I	CCSGG	1 cut(s)	187
AsuHPI	GGTGA	3 cut(s)	148, 232, 716
AvaII	GGWCC	1 cut(s)	755
BbvCI	CCTCAGC	1 cut(s)	817
BbvI	GCAGC	2 cut(s)	327, 482
BccI	CCATC	2 cut(s)	529, 766
BciT130I	CCWGG	1 cut(s)	570
BcnI	CCSGG	1 cut(s)	187
BfaI	CTAG	1 cut(s)	597
BfuAI	ACCTGC	1 cut(s)	566
BisI	GCNGC	4 cut(s)	316, 471, 590, 655
BlsI	GCNGC	4 cut(s)	317, 472, 591, 656
Bme1390I	CCNGG	2 cut(s)	187, 570
Bme18I	GGWCC	1 cut(s)	755
BmgT120I	GGNCC	1 cut(s)	755
BmiI	GGNNCC	1 cut(s)	756
BmrFI	CCNGG	2 cut(s)	187, 570
BmsI	GCATC	2 cut(s)	386, 517
BpmI	CTGGAG	2 cut(s)	363, 821
Bpu10I	CCTNAGC	1 cut(s)	817
BpuMI	CCSGG	1 cut(s)	187
BsaJI	CCNNGG	2 cut(s)	327, 569
Bsc4I	CCNNNNNNNGG	4 cut(s)	99, 186, 551, 575
Bse1I	ACTGG	3 cut(s)	643, 804, 925
Bse3DI	GCAATG	1 cut(s)	838
BseBI	CCWGG	1 cut(s)	570
BseDI	CCNNGG	2 cut(s)	327, 569
BseGI	GGATG	1 cut(s)	777
BseLI	CCNNNNNNNGG	4 cut(s)	99, 186, 551, 575
BseMI	GCAATG	1 cut(s)	838
BseMII	CTCAG	3 cut(s)	239, 831, 888
BseNI	ACTGG	3 cut(s)	643, 804, 925
BseRI	GAGGAG	1 cut(s)	75
BseXI	GCAGC	2 cut(s)	327, 482
BshFI	GGCC	1 cut(s)	214
BsiSI	CCGG	3 cut(s)	118, 186, 215
BslFI	GGGAC	1 cut(s)	810
BslI	CCNNNNNNNGG	4 cut(s)	99, 186, 551, 575
BsmFI	GGGAC	1 cut(s)	810
BsnI	GGCC	1 cut(s)	214
Bsp143I	GATC	3 cut(s)	309, 508, 868
BspACI	CCGC	4 cut(s)	9, 313, 589, 654
BspANI	GGCC	1 cut(s)	214
BspCNI	CTCAG	3 cut(s)	240, 830, 889
BspLI	GGNNCC	1 cut(s)	756
BspMI	ACCTGC	1 cut(s)	566
BspPI	GGATC	1 cut(s)	304
BsrDI	GCAATG	1 cut(s)	838
BsrI	ACTGG	3 cut(s)	643, 804, 925
BssECI	CCNNGG	2 cut(s)	327, 569
BssMI	GATC	3 cut(s)	309, 508, 868
BssT1I	CCWWGG	1 cut(s)	327
Bst2UI	CCWGG	1 cut(s)	570
Bst4CI	ACNGT	2 cut(s)	144, 692
BstC8I	GCNNGC	3 cut(s)	411, 542, 736
BstDEI	CTNAG	4 cut(s)	23, 248, 817, 897
BstENI	CCTNNNNNAGG	1 cut(s)	573
BstF5I	GGATG	1 cut(s)	777
BstKTI	GATC	3 cut(s)	312, 511, 871
BstMBI	GATC	3 cut(s)	309, 508, 868
BstMWI	GCNNNNNNNGC	3 cut(s)	707, 782, 887
BstNI	CCWGG	1 cut(s)	570
BstSCI	CCNGG	2 cut(s)	185, 568
BstV1I	GCAGC	2 cut(s)	327, 482
BsuRI	GGCC	1 cut(s)	214
BtsCI	GGATG	1 cut(s)	777
BtsI	GCAGTG	3 cut(s)	404, 537, 672
BtsIMutI	CAGTG	7 cut(s)	32, 286, 404, 537, 636, 672, 918
BveI	ACCTGC	1 cut(s)	566
Cac8I	GCNNGC	3 cut(s)	411, 542, 736
Cfr13I	GGNCC	1 cut(s)	755
Csp6I	GTAC	2 cut(s)	434, 916
CviAII	CATG	1 cut(s)	504
CviQI	GTAC	2 cut(s)	434, 916
DdeI	CTNAG	4 cut(s)	23, 248, 817, 897
DpnI	GATC	3 cut(s)	311, 510, 870
DpnII	GATC	3 cut(s)	309, 508, 868
DraI	TTTAAA	1 cut(s)	354
DraIII	CACNNNGTG	1 cut(s)	410
Eco130I	CCWWGG	1 cut(s)	327
Eco47I	GGWCC	1 cut(s)	755
Eco57I	CTGAAG	1 cut(s)	402
EcoNI	CCTNNNNNAGG	1 cut(s)	573
EcoRI	GAATTC	1 cut(s)	73
EcoRII	CCWGG	1 cut(s)	568
EcoT14I	CCWWGG	1 cut(s)	327
ErhI	CCWWGG	1 cut(s)	327
FaeI	CATG	1 cut(s)	507
FaiI	YATR	7 cut(s)	62, 245, 505, 520, 632, 743, 846
FaqI	GGGAC	1 cut(s)	810
FatI	CATG	1 cut(s)	503
Fnu4HI	GCNGC	4 cut(s)	316, 471, 590, 655
FokI	GGATG	1 cut(s)	784
Fsp4HI	GCNGC	4 cut(s)	316, 471, 590, 655
FspBI	CTAG	1 cut(s)	597
GluI	GCNGC	4 cut(s)	316, 471, 590, 655
GsuI	CTGGAG	2 cut(s)	363, 821
HaeIII	GGCC	1 cut(s)	214
HapII	CCGG	3 cut(s)	118, 186, 215
Hin1II	CATG	1 cut(s)	507
HincII	GTYRAC	1 cut(s)	400
HindII	GTYRAC	1 cut(s)	400
HinfI	GANTC	7 cut(s)	18, 121, 134, 168, 176, 182, 287
HpaII	CCGG	3 cut(s)	118, 186, 215
HphI	GGTGA	3 cut(s)	148, 232, 716
Hpy166II	GTNNAC	4 cut(s)	257, 400, 485, 918
Hpy188I	TCNGA	4 cut(s)	126, 181, 382, 516
Hpy188III	TCNNGA	3 cut(s)	68, 389, 830
Hpy8I	GTNNAC	4 cut(s)	257, 400, 485, 918
HpyAV	CCTTC	2 cut(s)	458, 745
HpyCH4III	ACNGT	2 cut(s)	144, 692
HpyCH4IV	ACGT	2 cut(s)	416, 854
HpyCH4V	TGCA	3 cut(s)	230, 377, 530
HpyF10VI	GCNNNNNNNGC	3 cut(s)	707, 782, 887
HpyF3I	CTNAG	4 cut(s)	23, 248, 817, 897
HpySE526I	ACGT	2 cut(s)	416, 854
Hsp92II	CATG	1 cut(s)	507
Kzo9I	GATC	3 cut(s)	309, 508, 868
LmnI	GCTCC	3 cut(s)	155, 344, 467
Lsp1109I	GCAGC	2 cut(s)	327, 482
LweI	GCATC	2 cut(s)	386, 517
MaeI	CTAG	1 cut(s)	597
MaeII	ACGT	2 cut(s)	416, 854
MaeIII	GTNAC	3 cut(s)	30, 144, 154
MalI	GATC	3 cut(s)	311, 510, 870
MboI	GATC	3 cut(s)	309, 508, 868
MboII	GAAGA	5 cut(s)	282, 503, 536, 658, 845
MfeI	CAATTG	2 cut(s)	231, 450
MluCI	AATT	4 cut(s)	73, 231, 450, 685
MlyI	GAGTC	4 cut(s)	27, 115, 162, 170
MmeI	TCCRAC	4 cut(s)	117, 186, 470, 586
MseI	TTAA	3 cut(s)	14, 353, 928
MslI	CAYNNNNRTG	1 cut(s)	843
MspA1I	CMGCKG	1 cut(s)	589
MspI	CCGG	3 cut(s)	118, 186, 215
MspR9I	CCNGG	2 cut(s)	187, 570
MunI	CAATTG	2 cut(s)	231, 450
MvaI	CCWGG	1 cut(s)	570
MwoI	GCNNNNNNNGC	3 cut(s)	707, 782, 887
NciI	CCSGG	1 cut(s)	187
NdeII	GATC	3 cut(s)	309, 508, 868
NlaIII	CATG	1 cut(s)	507
NlaIV	GGNNCC	1 cut(s)	756
NmuCI	GTSAC	3 cut(s)	30, 144, 154
PaqCI	CACCTGC	1 cut(s)	566
PfeI	GAWTC	3 cut(s)	134, 182, 287
PflMI	CCANNNNNTGG	1 cut(s)	99
PfoI	TCCNGGA	1 cut(s)	185
PkrI	GCNGC	4 cut(s)	317, 472, 591, 656
PleI	GAGTC	4 cut(s)	26, 115, 162, 170
PpsI	GAGTC	4 cut(s)	26, 115, 162, 170
Psp6I	CCWGG	1 cut(s)	568
PspGI	CCWGG	1 cut(s)	568
PspN4I	GGNNCC	1 cut(s)	756
PspPI	GGNCC	1 cut(s)	755
RsaI	GTAC	2 cut(s)	435, 917
RsaNI	GTAC	2 cut(s)	434, 916
RseI	CAYNNNNRTG	1 cut(s)	843
SaqAI	TTAA	3 cut(s)	14, 353, 928
SatI	GCNGC	4 cut(s)	316, 471, 590, 655
Sau3AI	GATC	3 cut(s)	309, 508, 868
Sau96I	GGNCC	1 cut(s)	755
SchI	GAGTC	4 cut(s)	27, 115, 162, 170
ScrFI	CCNGG	2 cut(s)	187, 570
SfaNI	GCATC	2 cut(s)	386, 517
SinI	GGWCC	1 cut(s)	755
SmiMI	CAYNNNNRTG	1 cut(s)	843
Sse9I	AATT	4 cut(s)	73, 231, 450, 685
SsiI	CCGC	4 cut(s)	9, 313, 589, 654
SspMI	CTAG	1 cut(s)	597
StyD4I	CCNGG	2 cut(s)	185, 568
StyI	CCWWGG	1 cut(s)	327
TaaI	ACNGT	2 cut(s)	144, 692
TaiI	ACGT	2 cut(s)	419, 857
TaqI	TCGA	2 cut(s)	388, 671
TaqII	GACCGA	1 cut(s)	18
TasI	AATT	4 cut(s)	73, 231, 450, 685
TatI	WGTACW	1 cut(s)	915
TauI	GCSGC	2 cut(s)	592, 657
TfiI	GAWTC	3 cut(s)	134, 182, 287
Tru1I	TTAA	3 cut(s)	14, 353, 928
Tru9I	TTAA	3 cut(s)	14, 353, 928
TscAI	CASTG	7 cut(s)	39, 286, 411, 537, 643, 672, 925
TseFI	GTSAC	3 cut(s)	30, 144, 154
TseI	GCWGC	2 cut(s)	315, 470
Tsp45I	GTSAC	3 cut(s)	30, 144, 154
TspDTI	ATGAA	1 cut(s)	861
TspGWI	ACGGA	2 cut(s)	142, 376
TspRI	CASTG	7 cut(s)	39, 286, 411, 537, 643, 672, 925
Van91I	CCANNNNNTGG	1 cut(s)	99
VpaK11BI	GGWCC	1 cut(s)	755
XagI	CCTNNNNNAGG	1 cut(s)	573
XapI	RAATTY	1 cut(s)	73
XspI	CTAG	1 cut(s)	597

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.