Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr7C

Orthogroup ID

OG0013516

Physical Location & Seq

Genomic Coordinates Reverse (-)

21319865 .. 21336033

Gene Structure 16169 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh7CG228700.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1314 bp

ATGGTCATAACTCACAACTCACTAGTAGAATTTACTGATCGCTGGAAGTTTCTCTATGAAGCAAGGAAGTGGTTAAAGCTAGTAGAGCATATATTGGCCATGATAGATTCAGTTATGGCCTTAATGATAGTTTGGGGGATTTTGATGAGCACAATTTATTTGGATATCTTGATTTTCAAAGATTCAGGACCTGATGACTCTTCCTTGGTGCCAAGCCAAGCAGTGAAACCTTTTGGTGTCGCCGGACGGGAGCCTAGTCGAGTCTGTGCACTGGAGGACATCGCGGCTCAGATGCTTCTTCGTCTTGGTGGTGTTTCGTCTAGTCGTTTAAGATGGCAGGCAGAGAGAGTCCAGTCACTGCTTCGTCAGTGTGGAACAAAAATCATGGGTGCTTTCAGAGTTCTAAGCAAGTCCCTCAGACATTACACACCCAAAGCCTCTATATTTCCAGAGAATTCAATCACTCCCAGGCCCAACAAATGGCTTCACCAAACTCGCCGGACTCTGATTCTAGAGTCAGCACCCTCTCAGTCCGTCAACCTCCACAGGCTCTCCGACTCCGATTCCGGAATCGTTGAAGTTAGTTTGGAGAGACCCCAAGTGAGAAATGCAATAGGGAAAGATATGCTCAAAGGTTTGAAGAACACGTTTGAAGCTATCAGTAAAGATTCATCTGCTAATGTTTTGTTGATCCGCAGTAAAGTTCCCAAGGTGTTCTGTGCAGGAGCAGATTTGAAGGAACGCAAGATGATGAGTGCAACTGAGGTTCAAGATTTCGTGAACAAGCTGCGTGCCACATTCTCATTTCTAGAGGTACTTCCCATTCCTACCATCGCTGTCATTGAAGGGGCAGCATTGGGTGGTGGACTTGAAATGGCTTTATCATGCGATCTTCGGATATGTGGAGAAGATGCAGTATTGGGATTGCCAGAAACTGGACTTGCTATAATTCCCGGGGCAGGTGGGACTCAACGACTTCCTAGAGTGGTTGGAAGATCAATAGCAAAGGAACTCATATTGACTGGGAGGAAGATTGGTGGCAGAGAAGCAATGTCAATGGGTCTGGTAAATTACTGTGTCCATGCTGGTGAAGCTCATCTAAAGGCACTTGAAGTTGCTCGCAATATAAATGAGAAGGGTCCAATAGCAATAAGGATGGCGAAAAGAGCTATTGACCAGGGACTTGAGGTAGATAGGGCATCAGCTTTGGCACTGGAAGAAGAATGCTATGAGCAGACCTTGCACACAGAAGATCGTTTGGAAGCCCTGGCAGCATTTGCTGAGAAGCGGAAGCCAAGGTATACTGGAAAATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004300 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0006082 GO:0006629 GO:0006631 GO:0006635 GO:0008150 GO:0008152 GO:0009056 GO:0009062 GO:0009987 GO:0016042 GO:0016054 GO:0016829 GO:0016835 GO:0016836 GO:0019395 GO:0019752 GO:0030258 GO:0032787 GO:0034440 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0044237 GO:0044238 GO:0044242 GO:0044248 GO:0044255 GO:0044281 GO:0044282 GO:0044424 GO:0044444 GO:0044464 GO:0046395 GO:0055114 GO:0071704 GO:0072329 GO:1901575

KEGG Pathways

Metabolic & Signaling

ko00280 ko01100 map00280 map01100

Pfam Domains

Protein Families

ECH_1

Protein Analysis

437

Amino Acids

48.15

Weight (kDa)

9.22

Isoelectric Point (pI)

47.89

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
ECH_1	PF00378	188 - 436	3.2e-61	Enoyl-CoA hydratase/isomerase
ECH_2	PF16113	194 - 364	1.4e-31	Enoyl-CoA hydratase/isomerase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0013516)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G16800
fragaria_vesca	FvH4_5g19890
malus_domestica	MD04G1057400.v1.1
prunus_persica	Prupe.5G061300_v2.0.a1 Prupe.5G061300_v2.0.a1
pyrus_communis	pycom04g05100
rosa_chinensis	RchiOBHm_Chr7g0204691
rosa_laevigata	RLG00000003437
rosa_multiflora	Rmu_sc0003221.1_g000066 Rmu_sc0007104.1_g000007
rosa_roxburghii	Rroxscaffold_3G00252860
rosa_rugosa	Rorug07G0085700 Rorug07G0085800
rosa_samantha	Rh7AG215600 Rh7BG212800 Rh7CG228700 Rh7DG223300
rosa_wichuraiana	Rw7G018690

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	950
Acc36I	ACCTGC	1 cut(s)	950
AccB1I	GGYRCC	1 cut(s)	208
AccB7I	CCANNNNNTGG	2 cut(s)	480, 935
AccI	GTMKAC	1 cut(s)	1301
AccII	CGCG	1 cut(s)	284
AccIII	TCCGGA	1 cut(s)	566
AciI	CCGC	3 cut(s)	284, 694, 1288
AclWI	GGATC	1 cut(s)	685
AcoI	YGGCCR	1 cut(s)	96
AcsI	RAATTY	2 cut(s)	29, 454
AfaI	GTAC	1 cut(s)	816
AfiI	CCNNNNNNNGG	3 cut(s)	480, 935, 959
AflIII	ACRYGT	1 cut(s)	645
AhlI	ACTAGT	1 cut(s)	22
AjnI	CCWGG	3 cut(s)	467, 1176, 1266
AluBI	AGCT	6 cut(s)	79, 656, 787, 1094, 1169, 1205
AluI	AGCT	6 cut(s)	79, 656, 787, 1094, 1169, 1205
Alw21I	GWGCWC	2 cut(s)	152, 271
Alw26I	GTCTC	1 cut(s)	586
Alw44I	GTGCAC	1 cut(s)	267
AlwI	GGATC	1 cut(s)	685
AlwNI	CAGNNNCTG	3 cut(s)	191, 358, 935
Ama87I	CYCGRG	1 cut(s)	953
Aor13HI	TCCGGA	1 cut(s)	566
AoxI	GGCC	3 cut(s)	96, 117, 470
ApaLI	GTGCAC	1 cut(s)	267
ApeKI	GCWGC	3 cut(s)	787, 851, 1271
ApoI	RAATTY	2 cut(s)	29, 454
AspS9I	GGNCC	3 cut(s)	188, 471, 1139
AsuC2I	CCSGG	2 cut(s)	954, 955
AsuHPI	GGTGA	2 cut(s)	479, 1100
AvaI	CYCGRG	1 cut(s)	953
AvaII	GGWCC	2 cut(s)	188, 1139
BaeGI	GKGCMC	1 cut(s)	271
BalI	TGGCCA	1 cut(s)	98
BanI	GGYRCC	1 cut(s)	208
Bbv12I	GWGCWC	2 cut(s)	152, 271
BbvI	GCAGC	3 cut(s)	774, 863, 1283
BccI	CCATC	3 cut(s)	327, 839, 1150
BciT130I	CCWGG	3 cut(s)	469, 1178, 1268
BcnI	CCSGG	2 cut(s)	954, 955
BcoDI	GTCTC	1 cut(s)	586
BcuI	ACTAGT	1 cut(s)	22
BfaI	CTAG	7 cut(s)	23, 80, 255, 321, 512, 809, 981
BfuAI	ACCTGC	1 cut(s)	950
BisI	GCNGC	4 cut(s)	285, 788, 852, 1272
BlsI	GCNGC	4 cut(s)	286, 789, 853, 1273
Bme1390I	CCNGG	5 cut(s)	469, 954, 955, 1178, 1268
Bme18I	GGWCC	2 cut(s)	188, 1139
BmeT110I	CYCGRG	1 cut(s)	953
BmgT120I	GGNCC	3 cut(s)	188, 471, 1139
BmiI	GGNNCC	3 cut(s)	210, 252, 1140
BmrFI	CCNGG	5 cut(s)	469, 954, 955, 1178, 1268
BmrI	ACTGGG	1 cut(s)	1032
BmsI	GCATC	3 cut(s)	282, 901, 1208
BmuI	ACTGGG	1 cut(s)	1032
BpmI	CTGGAG	1 cut(s)	293
BpuEI	CTTGAG	1 cut(s)	1205
BpuMI	CCSGG	2 cut(s)	954, 955
BsaI	GGTCTC	1 cut(s)	586
BsaJI	CCNNGG	8 cut(s)	204, 467, 708, 953, 954, 1177, 1266, 1295
BsaWI	WCCGGW	1 cut(s)	566
BsaXI	ACNNNNNCTCC	2 cut(s)	536, 566
Bsc4I	CCNNNNNNNGG	3 cut(s)	480, 935, 959
Bse1I	ACTGG	6 cut(s)	276, 352, 940, 1027, 1218, 1309
Bse3DI	GCAATG	1 cut(s)	1056
BseAI	TCCGGA	1 cut(s)	566
BseBI	CCWGG	3 cut(s)	469, 1178, 1268
BseDI	CCNNGG	8 cut(s)	204, 467, 708, 953, 954, 1177, 1266, 1295
BseGI	GGATG	1 cut(s)	1161
BseLI	CCNNNNNNNGG	3 cut(s)	480, 935, 959
BseMI	GCAATG	1 cut(s)	1056
BseMII	CTCAG	5 cut(s)	302, 430, 542, 753, 1272
BseNI	ACTGG	6 cut(s)	276, 352, 940, 1027, 1218, 1309
BseSI	GKGCMC	1 cut(s)	271
BseXI	GCAGC	3 cut(s)	774, 863, 1283
BsgI	GTGCAG	1 cut(s)	741
Bsh1236I	CGCG	1 cut(s)	284
BshFI	GGCC	3 cut(s)	98, 119, 472
BshNI	GGYRCC	1 cut(s)	208
BsiHKAI	GWGCWC	2 cut(s)	152, 271
BsiHKCI	CYCGRG	1 cut(s)	953
BsiSI	CCGG	4 cut(s)	243, 499, 567, 954
BslFI	GGGAC	3 cut(s)	397, 979, 1194
BslI	CCNNNNNNNGG	3 cut(s)	480, 935, 959
BsmAI	GTCTC	1 cut(s)	586
BsmFI	GGGAC	3 cut(s)	397, 979, 1194
BsmI	GAATGC	1 cut(s)	1229
BsnI	GGCC	3 cut(s)	98, 119, 472
Bso31I	GGTCTC	1 cut(s)	586
BsoBI	CYCGRG	1 cut(s)	953
Bsp1286I	GDGCHC	2 cut(s)	152, 271
Bsp13I	TCCGGA	1 cut(s)	566
Bsp143I	GATC	5 cut(s)	37, 690, 889, 995, 1252
BspACI	CCGC	3 cut(s)	284, 694, 1288
BspANI	GGCC	3 cut(s)	98, 119, 472
BspCNI	CTCAG	5 cut(s)	301, 429, 541, 754, 1273
BspEI	TCCGGA	1 cut(s)	566
BspFNI	CGCG	1 cut(s)	284
BspLI	GGNNCC	3 cut(s)	210, 252, 1140
BspMI	ACCTGC	1 cut(s)	950
BspPI	GGATC	1 cut(s)	685
BspT107I	GGYRCC	1 cut(s)	208
BspTNI	GGTCTC	1 cut(s)	586
BsrDI	GCAATG	1 cut(s)	1056
BsrI	ACTGG	6 cut(s)	276, 352, 940, 1027, 1218, 1309
BssECI	CCNNGG	8 cut(s)	204, 467, 708, 953, 954, 1177, 1266, 1295
BssMI	GATC	5 cut(s)	37, 690, 889, 995, 1252
BssNAI	GTATAC	1 cut(s)	1302
BssT1I	CCWWGG	3 cut(s)	204, 708, 1295
Bst1107I	GTATAC	1 cut(s)	1302
Bst2UI	CCWGG	3 cut(s)	469, 1178, 1268
Bst4CI	ACNGT	1 cut(s)	1076
Bst6I	CTCTTC	1 cut(s)	205
BstAPI	GCANNNNNTGC	2 cut(s)	1240, 1277
BstC8I	GCNNGC	3 cut(s)	339, 792, 1120
BstDEI	CTNAG	6 cut(s)	288, 404, 416, 528, 762, 1281
BstF5I	GGATG	1 cut(s)	1161
BstFNI	CGCG	1 cut(s)	284
BstKTI	GATC	5 cut(s)	40, 693, 892, 998, 1255
BstMAI	GTCTC	1 cut(s)	586
BstMBI	GATC	5 cut(s)	37, 690, 889, 995, 1252
BstMWI	GCNNNNNNNGC	6 cut(s)	85, 1091, 1166, 1240, 1271, 1277
BstNI	CCWGG	3 cut(s)	469, 1178, 1268
BstSCI	CCNGG	5 cut(s)	467, 952, 953, 1176, 1266
BstSLI	GKGCMC	1 cut(s)	271
BstUI	CGCG	1 cut(s)	284
BstV1I	GCAGC	3 cut(s)	774, 863, 1283
BstZ17I	GTATAC	1 cut(s)	1302
BsuRI	GGCC	3 cut(s)	98, 119, 472
BtgZI	GCGATG	2 cut(s)	265, 817
BtsCI	GGATG	1 cut(s)	1161
BtsI	GCAGTG	2 cut(s)	228, 356
BtsIMutI	CAGTG	5 cut(s)	228, 269, 356, 374, 1211
BveI	ACCTGC	1 cut(s)	950
Cac8I	GCNNGC	3 cut(s)	339, 792, 1120
CaiI	CAGNNNCTG	3 cut(s)	191, 358, 935
Cfr13I	GGNCC	3 cut(s)	188, 471, 1139
Cfr9I	CCCGGG	1 cut(s)	953
Csp6I	GTAC	1 cut(s)	815
CviAII	CATG	4 cut(s)	100, 385, 885, 1082
CviQI	GTAC	1 cut(s)	815
DdeI	CTNAG	6 cut(s)	288, 404, 416, 528, 762, 1281
DpnI	GATC	5 cut(s)	39, 692, 891, 997, 1254
DpnII	GATC	5 cut(s)	37, 690, 889, 995, 1252
EaeI	YGGCCR	1 cut(s)	96
Eam1104I	CTCTTC	1 cut(s)	205
EarI	CTCTTC	1 cut(s)	205
Eco130I	CCWWGG	3 cut(s)	204, 708, 1295
Eco31I	GGTCTC	1 cut(s)	586
Eco32I	GATATC	1 cut(s)	166
Eco47I	GGWCC	2 cut(s)	188, 1139
Eco88I	CYCGRG	1 cut(s)	953
EcoO109I	RGGNCCY	1 cut(s)	188
EcoRI	GAATTC	1 cut(s)	454
EcoRII	CCWGG	3 cut(s)	467, 1176, 1266
EcoRV	GATATC	1 cut(s)	166
EcoT14I	CCWWGG	3 cut(s)	204, 708, 1295
ErhI	CCWWGG	3 cut(s)	204, 708, 1295
FaeI	CATG	4 cut(s)	103, 388, 888, 1085
FaqI	GGGAC	3 cut(s)	397, 979, 1194
FatI	CATG	4 cut(s)	99, 384, 884, 1081
FblI	GTMKAC	1 cut(s)	1301
Fnu4HI	GCNGC	4 cut(s)	285, 788, 852, 1272
FokI	GGATG	1 cut(s)	1168
Fsp4HI	GCNGC	4 cut(s)	285, 788, 852, 1272
FspBI	CTAG	7 cut(s)	23, 80, 255, 321, 512, 809, 981
GluI	GCNGC	4 cut(s)	285, 788, 852, 1272
GsuI	CTGGAG	1 cut(s)	293
HaeIII	GGCC	3 cut(s)	98, 119, 472
HapII	CCGG	4 cut(s)	243, 499, 567, 954
Hin1II	CATG	4 cut(s)	103, 388, 888, 1085
HincII	GTYRAC	1 cut(s)	538
HindII	GTYRAC	1 cut(s)	538
HpaII	CCGG	4 cut(s)	243, 499, 567, 954
HphI	GGTGA	2 cut(s)	479, 1100
Hpy166II	GTNNAC	5 cut(s)	269, 538, 781, 866, 1302
Hpy188I	TCNGA	7 cut(s)	291, 398, 419, 507, 556, 562, 897
Hpy188III	TCNNGA	8 cut(s)	169, 186, 449, 512, 567, 770, 778, 809
Hpy8I	GTNNAC	5 cut(s)	269, 538, 781, 866, 1302
HpyAV	CCTTC	3 cut(s)	730, 839, 1129
HpyCH4III	ACNGT	1 cut(s)	1076
HpyCH4IV	ACGT	1 cut(s)	647
HpyCH4V	TGCA	6 cut(s)	269, 611, 722, 758, 914, 1243
HpyF10VI	GCNNNNNNNGC	6 cut(s)	85, 1091, 1166, 1240, 1271, 1277
HpyF3I	CTNAG	6 cut(s)	288, 404, 416, 528, 762, 1281
HpySE526I	ACGT	1 cut(s)	647
Hsp92II	CATG	4 cut(s)	103, 388, 888, 1085
Kpn2I	TCCGGA	1 cut(s)	566
Kzo9I	GATC	5 cut(s)	37, 690, 889, 995, 1252
LmnI	GCTCC	2 cut(s)	250, 725
Lsp1109I	GCAGC	3 cut(s)	774, 863, 1283
LweI	GCATC	3 cut(s)	282, 901, 1208
MaeI	CTAG	7 cut(s)	23, 80, 255, 321, 512, 809, 981
MaeII	ACGT	1 cut(s)	647
MaeIII	GTNAC	1 cut(s)	354
MalI	GATC	5 cut(s)	39, 692, 891, 997, 1254
MboI	GATC	5 cut(s)	37, 690, 889, 995, 1252
MhlI	GDGCHC	2 cut(s)	152, 271
MlsI	TGGCCA	1 cut(s)	98
MluCI	AATT	5 cut(s)	29, 153, 454, 948, 1069
MluNI	TGGCCA	1 cut(s)	98
MlyI	GAGTC	7 cut(s)	191, 270, 357, 496, 524, 551, 961
MmeI	TCCRAC	2 cut(s)	579, 970
MnlI	CCTC	9 cut(s)	268, 425, 448, 535, 551, 757, 805, 1020, 1180
Mox20I	TGGCCA	1 cut(s)	98
MroI	TCCGGA	1 cut(s)	566
MscI	TGGCCA	1 cut(s)	98
MseI	TTAA	3 cut(s)	74, 122, 329
MslI	CAYNNNNRTG	1 cut(s)	1086
Msp20I	TGGCCA	1 cut(s)	98
MspI	CCGG	4 cut(s)	243, 499, 567, 954
MspR9I	CCNGG	5 cut(s)	469, 954, 955, 1178, 1268
Mva1269I	GAATGC	1 cut(s)	1229
MvaI	CCWGG	3 cut(s)	469, 1178, 1268
MvnI	CGCG	1 cut(s)	284
MwoI	GCNNNNNNNGC	6 cut(s)	85, 1091, 1166, 1240, 1271, 1277
NciI	CCSGG	2 cut(s)	954, 955
NdeII	GATC	5 cut(s)	37, 690, 889, 995, 1252
NlaIII	CATG	4 cut(s)	103, 388, 888, 1085
NlaIV	GGNNCC	3 cut(s)	210, 252, 1140
NmuCI	GTSAC	1 cut(s)	354
PaqCI	CACCTGC	1 cut(s)	950
PctI	GAATGC	1 cut(s)	1229
PfeI	GAWTC	6 cut(s)	107, 182, 508, 563, 570, 668
PflMI	CCANNNNNTGG	2 cut(s)	480, 935
PkrI	GCNGC	4 cut(s)	286, 789, 853, 1273
PleI	GAGTC	7 cut(s)	191, 269, 356, 496, 523, 551, 961
PpsI	GAGTC	7 cut(s)	191, 269, 356, 496, 523, 551, 961
PpuMI	RGGWCCY	1 cut(s)	188
Psp5II	RGGWCCY	1 cut(s)	188
Psp6I	CCWGG	3 cut(s)	467, 1176, 1266
PspGI	CCWGG	3 cut(s)	467, 1176, 1266
PspN4I	GGNNCC	3 cut(s)	210, 252, 1140
PspPI	GGNCC	3 cut(s)	188, 471, 1139
PspPPI	RGGWCCY	1 cut(s)	188
PstNI	CAGNNNCTG	3 cut(s)	191, 358, 935
RsaI	GTAC	1 cut(s)	816
RsaNI	GTAC	1 cut(s)	815
RseI	CAYNNNNRTG	1 cut(s)	1086
SaqAI	TTAA	3 cut(s)	74, 122, 329
SatI	GCNGC	4 cut(s)	285, 788, 852, 1272
Sau3AI	GATC	5 cut(s)	37, 690, 889, 995, 1252
Sau96I	GGNCC	3 cut(s)	188, 471, 1139
SchI	GAGTC	7 cut(s)	191, 270, 357, 496, 524, 551, 961
ScrFI	CCNGG	5 cut(s)	469, 954, 955, 1178, 1268
SduI	GDGCHC	2 cut(s)	152, 271
SfaNI	GCATC	3 cut(s)	282, 901, 1208
SinI	GGWCC	2 cut(s)	188, 1139
SmaI	CCCGGG	1 cut(s)	955
SmiMI	CAYNNNNRTG	1 cut(s)	1086
SmlI	CTYRAG	1 cut(s)	1184
SmoI	CTYRAG	1 cut(s)	1184
SpeI	ACTAGT	1 cut(s)	22
Sse9I	AATT	5 cut(s)	29, 153, 454, 948, 1069
SsiI	CCGC	3 cut(s)	284, 694, 1288
SspMI	CTAG	7 cut(s)	23, 80, 255, 321, 512, 809, 981
StyD4I	CCNGG	5 cut(s)	467, 952, 953, 1176, 1266
StyI	CCWWGG	3 cut(s)	204, 708, 1295
TaaI	ACNGT	1 cut(s)	1076
TaiI	ACGT	1 cut(s)	650
TaqI	TCGA	1 cut(s)	259
TasI	AATT	5 cut(s)	29, 153, 454, 948, 1069
TauI	GCSGC	1 cut(s)	287
TfiI	GAWTC	6 cut(s)	107, 182, 508, 563, 570, 668
Tru1I	TTAA	3 cut(s)	74, 122, 329
Tru9I	TTAA	3 cut(s)	74, 122, 329
TscAI	CASTG	5 cut(s)	228, 276, 363, 374, 1218
TseFI	GTSAC	1 cut(s)	354
TseI	GCWGC	3 cut(s)	787, 851, 1271
Tsp45I	GTSAC	1 cut(s)	354
TspDTI	ATGAA	2 cut(s)	72, 660
TspGWI	ACGGA	1 cut(s)	523
TspMI	CCCGGG	1 cut(s)	953
TspRI	CASTG	5 cut(s)	228, 276, 363, 374, 1218
Van91I	CCANNNNNTGG	2 cut(s)	480, 935
VneI	GTGCAC	1 cut(s)	267
VpaK11BI	GGWCC	2 cut(s)	188, 1139
XapI	RAATTY	2 cut(s)	29, 454
XbaI	TCTAGA	2 cut(s)	511, 808
XmaI	CCCGGG	1 cut(s)	953
XmiI	GTMKAC	1 cut(s)	1301
XspI	CTAG	7 cut(s)	23, 80, 255, 321, 512, 809, 981

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.