Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr04

Orthogroup ID

OG0005128

Physical Location & Seq

Genomic Coordinates Reverse (-)

7812007 .. 7815504

Gene Structure 3498 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD04G1060300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2409 bp

ATGCTCCCGCACTCGTACGCCCTCAATTCCCATTCCAAATCCCAAGACCTCGCCTGCACCATCCTCGCTTCCTCCGCCCCGCACCAGATCTCCTCCACTTGCGCCTCCATCGAGTCTTTCCTGCACTCCCTCTCACCCGACCAGTCCCGCCACTTCTTCTCCCTCACCTTCCCAACCCTCATCTGCAAGCTCTTCGGCTTCGACGATGCAACGTCATCACCCCCGCCTCCGCCACAACCAGCTTCACAGCAACAACCCTCGCCCTCCGCCGCCTCCGCCTCACCCAACGGTTGGATTGACACCGTCCTCGCATCCAACGACATAGATTTAGCCAACAGGCTCTTCGCCCTCCTCGCGCCCAGTAGCCTCCTCTTCAACGCAATCTCCGCCGTTGATCGCCTCTCCCTCGTCAAATACGTGTTCCCTATTGAACGGTTGCCGGAATGGGTCCGGTTCATGCTTTCCTCGGAAAATGGTAGCCGGGTATTATCCGATTTATCCCCAATTTTCAAAAATAGGGTTAAAGAAGACTCAATCAAACCTAATTTCTATCAAGTTCAGCTCAATGTCTTTGAATATTACATGTTTTGGTTCGCTTATTACCCCGTTTGTCGAGGAAATAGTGAGAATTGTGATGTTGTTTCGATAAAAAGAAACAGAAGGTTTAAATTTGAGAATTGGGTTTCATCAATTTCAGGGTTTTCAAGCACTAGGCGTGGGCCGGAGGTTAAAATTGAATGCAACTTGTATATTAGGCTTTTATATGCATATCTTCGTGCTTTTGTTGGTGCAACTGATTTGAATCAACACCTTCCATACCGAAGTTCGCTTTTGCATTACTCTTCGGGATATGATACCTCGGTTATAGCTCAGGCAGAGGTTTTTGTTAATGCTTTGGTGAATTTTTGGTTGGTTGATAATGATTTTTCACCACTACCTGTTAATCTGTGTAAGTCATTTGGGGTCTCGTTCCCATTCCGGTCAGTTTTGGGTGAGACCCCTCCAACCCCCATGTTAGGAGAGGTGGTGAAGTTGTTTGTCAAATACTTGAATGTGGGTTTGGTCGTGCATAGAAATGGTAATGACAATGTGGAACATTGTGGCAGTCCGAGGCGGAGGGGTTCATTTGATGCCTTGAAGTTGAGGGATGTGATGTCAGTTTCACCATGTACAGGATCTTGGAACTTGTTGATACAGAGGCCGCTGTACCGGTTTATATTGAGGACATTCTTATTTTGTCCAGTGGGGGTTTCGATTAAAAATATATCAGAGGTGTTTTCGGTTTGGATTACCTATATTGAACCTTGGGATATCAGTTTGGATGACTTTTCAGAGCTTGATGCTGTTGTTGACGGATCAACTAAGAATGGGAGAAAGGATAACCTGCAGCCTCAAAACCATAGTTATACACCTTCTTGGCAGGGTTACGTGCTGGCCAGCTACTTGTACTACAGTTCCTTGGTTATGCATTTCATTGGGTTTGCGCACAAGTTTCTTCATACTGATCCCGAAATAATTGTCCAGATGGTATTGAAGATCCTAACTATATTGACATCATCCAAAGAGCTGATGGACCTATTAAAGATGGTGGATACTGCTTTTCATTCCAAACAAGCAGGATCAGGAAAATCGATGCTTAATAGCTTATATAGAATTGTTACTCCGATTCGCGAACAGCTGCAGGACTGGGAGGATGGCTTATCTGAGAGTGATGCAGATGGGTCTTTCTTGCATGAGAATTGGAACAAAGATTTACGATTGTTCAGTGATGGAGAAGATGGCGGGCAACAGTTGCTTCAGTTGTTCATATTACGTGCAGAAGCTGAGTTGCTAGCTATTTCCGGTGATAATGGTACGCGCAACCTTCAGTGCGTAGACTCTTTGAAAGCTCAGGTGAGCTGTCTATTTGGTGGGCATACTGTAAAGGTACTGTCATTTTCTCCTGAACCAAAACAGCCTCCACAACCCCGTGATGCAATATTCAAGCCCAGGAGAGTGGGCAACCGCACTTCGGATGATGTCAAATACAGGGGTGACTGGATGAAGCGCCCCATCTCCAACAATGAGGTTGCTTGGCTCGCGAGATTGCTTGTTGAGTTTTCCAGTTGGATAAATGAGCGTCTTGGACTAAACCAGTCAGAGAGCAGCCAAGCAGACCCTACATATGTGGAAGTGTCGAGCAATGTTTTGGGCAATGTCTTTGGACCGATGGATACGATGAAGGCGGTGAAGGGGGCAGTAGGTTCTTGGCTTGTGATGTTGGGAGTGGCAATGGTGAAGCTAATGAGGAAACATGGATTGAGGGTGAATCTGAGGATGTTGGCGTCAAAGAAGGTTGTAATGGTTTTGTTTTTGTCTGCTGTATATAGTATATTGAAAAAAGCTTTCGGAATGTACCAAAGGGTGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

ko00600 ko01100 map00600 map01100

Pfam Domains

Protein Families

mit_SMPDase

Protein Analysis

803

Amino Acids

90.25

Weight (kDa)

8.53

Isoelectric Point (pI)

47.18

Instability Index

Protein Domains (Pfam)

No Pfam domains detected for this protein.

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Font Size (px)

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0005128)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G47400
fragaria_vesca	FvH4_5g19410 FvH4_5g19410 FvH4_5g19410 FvH4_5g19410 FvH4_5g19410 FvH4_5g19410 FvH4_5g19410 FvH4_5g19410 FvH4_5g19410 FvH4_5g19410 FvH4_5g19410 FvH4_5g19410
malus_domestica	MD04G1060300.v1.1 MD06G1053300.v1.1
prunus_persica	Prupe.5G065400_v2.0.a1
pyrus_communis	pycom04g05390 pycom06g04500
rosa_chinensis	RchiOBHm_Chr7g0203921
rosa_laevigata	RLG00000003498
rosa_multiflora	Rmu_co8411937.1_g000001 Rmu_sc0013871.1_g000002 Rmu_sc0032022.1_g000002
rosa_roxburghii	Rroxscaffold_3G00253460
rosa_rugosa	Rorug07G0079400
rosa_samantha	Rh7AG209300 Rh7BG208100 Rh7CG221700 Rh7DG216400
rosa_wichuraiana	Rw7G018240

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	1485
Acc36I	ACCTGC	1 cut(s)	1392
AccB7I	CCANNNNNTGG	1 cut(s)	291
AccI	GTMKAC	1 cut(s)	1875
AccII	CGCG	4 cut(s)	356, 1671, 1858, 2081
AclWI	GGATC	5 cut(s)	1183, 1363, 1499, 1531, 1627
AcoI	YGGCCR	1 cut(s)	1434
AcsI	RAATTY	2 cut(s)	668, 901
AcuI	CTGAAG	2 cut(s)	1781, 1850
AcyI	GRCGYC	1 cut(s)	2324
AfaI	GTAC	7 cut(s)	17, 1171, 1208, 1448, 1855, 1929, 2396
AfiI	CCNNNNNNNGG	4 cut(s)	291, 1016, 1172, 2011
AflIII	ACRYGT	2 cut(s)	417, 582
AgeI	ACCGGT	1 cut(s)	1209
AjnI	CCWGG	1 cut(s)	1988
AjuI	GAANNNNNNNTTGG	6 cut(s)	326, 358, 893, 925, 1043, 1075
Alw26I	GTCTC	2 cut(s)	970, 989
AlwI	GGATC	5 cut(s)	1183, 1363, 1499, 1531, 1627
AlwNI	CAGNNNCTG	1 cut(s)	1823
AoxI	GGCC	3 cut(s)	719, 1199, 1434
ApeKI	GCWGC	3 cut(s)	1387, 1678, 2145
ApoI	RAATTY	2 cut(s)	668, 901
ArsI	GACNNNNNNTTYG	2 cut(s)	1503, 1535
AsiGI	ACCGGT	1 cut(s)	1209
AspLEI	GCGC	5 cut(s)	104, 358, 1486, 1860, 2049
AspS9I	GGNCC	4 cut(s)	448, 719, 1573, 2204
AsuC2I	CCSGG	1 cut(s)	482
AsuNHI	GCTAGC	1 cut(s)	1831
AvaII	GGWCC	3 cut(s)	448, 1573, 2204
BaeI	ACNNNNGTAYC	2 cut(s)	846, 879
BalI	TGGCCA	1 cut(s)	1436
BbsI	GAAGAC	1 cut(s)	534
BbvI	GCAGC	3 cut(s)	1399, 1665, 2157
BceAI	ACGGC	1 cut(s)	374
BcgI	CGANNNNNNTGC	4 cut(s)	46, 80, 175, 209
BciT130I	CCWGG	1 cut(s)	1990
BciVI	GTATCC	2 cut(s)	1585, 2206
BcnI	CCSGG	1 cut(s)	482
BcoDI	GTCTC	2 cut(s)	970, 989
BfaI	CTAG	2 cut(s)	711, 1832
BfmI	CTRYAG	3 cut(s)	1385, 1450, 1679
BfoI	RGCGCY	1 cut(s)	2050
BfuAI	ACCTGC	1 cut(s)	1392
BfuI	GTATCC	2 cut(s)	1585, 2206
BglII	AGATCT	1 cut(s)	87
BisI	GCNGC	5 cut(s)	270, 1202, 1388, 1679, 2146
BlsI	GCNGC	5 cut(s)	271, 1203, 1389, 1680, 2147
Bme1390I	CCNGG	2 cut(s)	482, 1990
Bme18I	GGWCC	3 cut(s)	448, 1573, 2204
BmgT120I	GGNCC	4 cut(s)	448, 719, 1573, 2204
BmiI	GGNNCC	1 cut(s)	449
BmrFI	CCNGG	2 cut(s)	482, 1990
BmrI	ACTGGG	2 cut(s)	354, 1696
BmsI	GCATC	7 cut(s)	196, 320, 1120, 1330, 1623, 1702, 1963
BmtI	GCTAGC	1 cut(s)	1835
BmuI	ACTGGG	2 cut(s)	354, 1696
BpiI	GAAGAC	1 cut(s)	534
Bpu10I	CCTNAGC	2 cut(s)	870, 1890
BpuMI	CCSGG	1 cut(s)	482
Bsa29I	ATCGAT	1 cut(s)	1631
BsaAI	YACGTR	3 cut(s)	418, 1429, 1814
BsaBI	GATNNNNATC	1 cut(s)	801
BsaHI	GRCGYC	1 cut(s)	2324
BsaI	GGTCTC	2 cut(s)	970, 989
BsaJI	CCNNGG	6 cut(s)	465, 858, 1109, 1304, 1458, 1988
BsaWI	WCCGGW	4 cut(s)	450, 978, 1209, 1841
BsaXI	ACNNNNNCTCC	8 cut(s)	74, 104, 143, 173, 368, 398, 1011, 1041
Bsc4I	CCNNNNNNNGG	4 cut(s)	291, 1016, 1172, 2011
Bse118I	RCCGGY	1 cut(s)	1209
Bse1I	ACTGG	7 cut(s)	142, 360, 1241, 1691, 2042, 2103, 2134
Bse3DI	GCAATG	3 cut(s)	2188, 2200, 2277
Bse8I	GATNNNNATC	1 cut(s)	801
BseBI	CCWGG	1 cut(s)	1990
BseCI	ATCGAT	1 cut(s)	1631
BseDI	CCNNGG	6 cut(s)	465, 858, 1109, 1304, 1458, 1988
BseGI	GGATG	9 cut(s)	60, 311, 1153, 1327, 1556, 1699, 2020, 2046, 2322
BseJI	GATNNNNATC	1 cut(s)	801
BseLI	CCNNNNNNNGG	4 cut(s)	291, 1016, 1172, 2011
BseMI	GCAATG	3 cut(s)	2188, 2200, 2277
BseMII	CTCAG	5 cut(s)	884, 1695, 1815, 1904, 2303
BseNI	ACTGG	7 cut(s)	142, 360, 1241, 1691, 2042, 2103, 2134
BseRI	GAGGAG	3 cut(s)	82, 341, 359
BseXI	GCAGC	3 cut(s)	1399, 1665, 2157
BsgI	GTGCAG	3 cut(s)	40, 107, 1836
Bsh1236I	CGCG	4 cut(s)	356, 1671, 1858, 2081
BshFI	GGCC	3 cut(s)	721, 1201, 1436
BshTI	ACCGGT	1 cut(s)	1209
BshVI	ATCGAT	1 cut(s)	1631
BsiSI	CCGG	7 cut(s)	440, 451, 481, 722, 979, 1210, 1842
BsiWI	CGTACG	1 cut(s)	15
BslFI	GGGAC	1 cut(s)	130
BslI	CCNNNNNNNGG	4 cut(s)	291, 1016, 1172, 2011
BsmAI	GTCTC	2 cut(s)	970, 989
BsmFI	GGGAC	1 cut(s)	130
BsmI	GAATGC	1 cut(s)	743
BsnI	GGCC	3 cut(s)	721, 1201, 1436
Bso31I	GGTCTC	2 cut(s)	970, 989
Bsp1407I	TGTACA	1 cut(s)	1169
Bsp143I	GATC	7 cut(s)	87, 394, 1175, 1355, 1504, 1536, 1619
Bsp68I	TCGCGA	2 cut(s)	1671, 2081
BspANI	GGCC	3 cut(s)	721, 1201, 1436
BspCNI	CTCAG	5 cut(s)	883, 1696, 1816, 1903, 2304
BspDI	ATCGAT	1 cut(s)	1631
BspFNI	CGCG	4 cut(s)	356, 1671, 1858, 2081
BspLI	GGNNCC	1 cut(s)	449
BspMAI	CTGCAG	2 cut(s)	1389, 1683
BspMI	ACCTGC	1 cut(s)	1392
BspOI	GCTAGC	1 cut(s)	1835
BspPI	GGATC	5 cut(s)	1183, 1363, 1499, 1531, 1627
BspQI	GCTCTTC	2 cut(s)	197, 347
BspTNI	GGTCTC	2 cut(s)	970, 989
BsrDI	GCAATG	3 cut(s)	2188, 2200, 2277
BsrFI	RCCGGY	1 cut(s)	1209
BsrGI	TGTACA	1 cut(s)	1169
BsrI	ACTGG	7 cut(s)	142, 360, 1241, 1691, 2042, 2103, 2134
BssAI	RCCGGY	1 cut(s)	1209
BssECI	CCNNGG	6 cut(s)	465, 858, 1109, 1304, 1458, 1988
BssMI	GATC	7 cut(s)	87, 394, 1175, 1355, 1504, 1536, 1619
BssNI	GRCGYC	1 cut(s)	2324
BssT1I	CCWWGG	2 cut(s)	1304, 1458
Bst2UI	CCWGG	1 cut(s)	1990
Bst4CI	ACNGT	7 cut(s)	290, 304, 435, 1454, 1791, 1921, 1932
Bst6I	CTCTTC	4 cut(s)	197, 347, 377, 847
BstACI	GRCGYC	1 cut(s)	2324
BstAPI	GCANNNNNTGC	1 cut(s)	1792
BstAUI	TGTACA	1 cut(s)	1169
BstBAI	YACGTR	3 cut(s)	418, 1429, 1814
BstC8I	GCNNGC	7 cut(s)	55, 188, 1434, 1438, 1784, 1833, 2079
BstDEI	CTNAG	6 cut(s)	870, 1362, 1704, 1824, 1890, 2312
BstF5I	GGATG	9 cut(s)	60, 311, 1153, 1327, 1556, 1699, 2020, 2046, 2322
BstFNI	CGCG	4 cut(s)	356, 1671, 1858, 2081
BstH2I	RGCGCY	1 cut(s)	2050
BstHHI	GCGC	5 cut(s)	104, 358, 1486, 1860, 2049
BstKTI	GATC	7 cut(s)	90, 397, 1178, 1358, 1507, 1539, 1622
BstMAI	GTCTC	2 cut(s)	970, 989
BstMBI	GATC	7 cut(s)	87, 394, 1175, 1355, 1504, 1536, 1619
BstMWI	GCNNNNNNNGC	6 cut(s)	74, 275, 353, 386, 1792, 2078
BstNI	CCWGG	1 cut(s)	1990
BstNSI	RCATGY	1 cut(s)	586
BstSCI	CCNGG	2 cut(s)	480, 1988
BstSFI	CTRYAG	3 cut(s)	1385, 1450, 1679
BstUI	CGCG	4 cut(s)	356, 1671, 1858, 2081
BstV1I	GCAGC	3 cut(s)	1399, 1665, 2157
BstV2I	GAAGAC	1 cut(s)	534
BstX2I	RGATCY	3 cut(s)	87, 1175, 1536
BstXI	CCANNNNNNTGG	1 cut(s)	1996
BstYI	RGATCY	3 cut(s)	87, 1175, 1536
Bsu15I	ATCGAT	1 cut(s)	1631
BsuI	GTATCC	2 cut(s)	1585, 2206
BsuRI	GGCC	3 cut(s)	721, 1201, 1436
BsuTUI	ATCGAT	1 cut(s)	1631
BtsCI	GGATG	9 cut(s)	60, 311, 1153, 1327, 1556, 1699, 2020, 2046, 2322
BtsIMutI	CAGTG	3 cut(s)	1248, 1771, 1874
BtuMI	TCGCGA	2 cut(s)	1671, 2081
BveI	ACCTGC	1 cut(s)	1392
Cac8I	GCNNGC	7 cut(s)	55, 188, 1434, 1438, 1784, 1833, 2079
CaiI	CAGNNNCTG	1 cut(s)	1823
CfoI	GCGC	5 cut(s)	104, 358, 1486, 1860, 2049
Cfr10I	RCCGGY	1 cut(s)	1209
Cfr13I	GGNCC	4 cut(s)	448, 719, 1573, 2204
ClaI	ATCGAT	1 cut(s)	1631
CseI	GACGC	2 cut(s)	2108, 2313
Csp6I	GTAC	7 cut(s)	16, 1170, 1207, 1447, 1854, 1928, 2395
CspAI	ACCGGT	1 cut(s)	1209
CviAII	CATG	6 cut(s)	457, 583, 1012, 1167, 1733, 2294
CviQI	GTAC	7 cut(s)	16, 1170, 1207, 1447, 1854, 1928, 2395
DdeI	CTNAG	6 cut(s)	870, 1362, 1704, 1824, 1890, 2312
DpnI	GATC	7 cut(s)	89, 396, 1177, 1357, 1506, 1538, 1621
DpnII	GATC	7 cut(s)	87, 394, 1175, 1355, 1504, 1536, 1619
DraI	TTTAAA	1 cut(s)	667
EaeI	YGGCCR	1 cut(s)	1434
Eam1104I	CTCTTC	4 cut(s)	197, 347, 377, 847
EarI	CTCTTC	4 cut(s)	197, 347, 377, 847
EciI	GGCGGA	6 cut(s)	64, 219, 256, 265, 376, 1129
Eco130I	CCWWGG	2 cut(s)	1304, 1458
Eco31I	GGTCTC	2 cut(s)	970, 989
Eco32I	GATATC	1 cut(s)	1312
Eco47I	GGWCC	3 cut(s)	448, 1573, 2204
Eco57I	CTGAAG	2 cut(s)	1781, 1850
EcoRII	CCWGG	1 cut(s)	1988
EcoRV	GATATC	1 cut(s)	1312
EcoT14I	CCWWGG	2 cut(s)	1304, 1458
EcoT22I	ATGCAT	2 cut(s)	769, 1470
ErhI	CCWWGG	2 cut(s)	1304, 1458
FaeI	CATG	6 cut(s)	460, 586, 1015, 1170, 1736, 2297
FaqI	GGGAC	1 cut(s)	130
FatI	CATG	6 cut(s)	456, 582, 1011, 1166, 1732, 2293
FauI	CCCGC	5 cut(s)	15, 87, 155, 231, 1775
FauNDI	CATATG	1 cut(s)	2164
FblI	GTMKAC	1 cut(s)	1875
Fnu4HI	GCNGC	5 cut(s)	270, 1202, 1388, 1679, 2146
FokI	GGATG	9 cut(s)	47, 298, 1160, 1334, 1543, 1706, 2027, 2053, 2329
Fsp4HI	GCNGC	5 cut(s)	270, 1202, 1388, 1679, 2146
FspBI	CTAG	2 cut(s)	711, 1832
FspI	TGCGCA	1 cut(s)	1485
GlaI	GCGC	5 cut(s)	103, 357, 1485, 1859, 2048
GluI	GCNGC	5 cut(s)	270, 1202, 1388, 1679, 2146
HaeII	RGCGCY	1 cut(s)	2050
HaeIII	GGCC	3 cut(s)	721, 1201, 1436
HapII	CCGG	7 cut(s)	440, 451, 481, 722, 979, 1210, 1842
HgaI	GACGC	2 cut(s)	2108, 2313
HhaI	GCGC	5 cut(s)	104, 358, 1486, 1860, 2049
Hin1I	GRCGYC	1 cut(s)	2324
Hin1II	CATG	6 cut(s)	460, 586, 1015, 1170, 1736, 2297
Hin6I	GCGC	5 cut(s)	102, 356, 1484, 1858, 2047
HinP1I	GCGC	5 cut(s)	102, 356, 1484, 1858, 2047
HincII	GTYRAC	1 cut(s)	1351
HindII	GTYRAC	1 cut(s)	1351
HindIII	AAGCTT	1 cut(s)	2382
HinfI	GANTC	6 cut(s)	113, 530, 802, 1666, 1877, 2308
HpaII	CCGG	7 cut(s)	440, 451, 481, 722, 979, 1210, 1842
Hpy166II	GTNNAC	2 cut(s)	1351, 1876
Hpy188III	TCNNGA	7 cut(s)	846, 1508, 1522, 1623, 1670, 1943, 2080
Hpy8I	GTNNAC	2 cut(s)	1351, 1876
Hpy99I	CGWCG	1 cut(s)	206
HpyAV	CCTTC	8 cut(s)	178, 654, 821, 1422, 1874, 2215, 2224, 2326
HpyCH4III	ACNGT	7 cut(s)	290, 304, 435, 1454, 1791, 1921, 1932
HpyCH4IV	ACGT	4 cut(s)	212, 417, 1428, 1813
HpyF10VI	GCNNNNNNNGC	6 cut(s)	74, 275, 353, 386, 1792, 2078
HpyF3I	CTNAG	6 cut(s)	870, 1362, 1704, 1824, 1890, 2312
HpySE526I	ACGT	4 cut(s)	212, 417, 1428, 1813
Hsp92I	GRCGYC	1 cut(s)	2324
Hsp92II	CATG	6 cut(s)	460, 586, 1015, 1170, 1736, 2297
HspAI	GCGC	5 cut(s)	102, 356, 1484, 1858, 2047
Kzo9I	GATC	7 cut(s)	87, 394, 1175, 1355, 1504, 1536, 1619
LguI	GCTCTTC	2 cut(s)	197, 347
LmnI	GCTCC	1 cut(s)	9
Lsp1109I	GCAGC	3 cut(s)	1399, 1665, 2157
LweI	GCATC	7 cut(s)	196, 320, 1120, 1330, 1623, 1702, 1963
MaeI	CTAG	2 cut(s)	711, 1832
MaeII	ACGT	4 cut(s)	212, 417, 1428, 1813
MaeIII	GTNAC	3 cut(s)	1424, 1657, 2033
MalI	GATC	7 cut(s)	89, 396, 1177, 1357, 1506, 1538, 1621
MboI	GATC	7 cut(s)	87, 394, 1175, 1355, 1504, 1536, 1619
MflI	RGATCY	3 cut(s)	87, 1175, 1536
MlsI	TGGCCA	1 cut(s)	1436
MluNI	TGGCCA	1 cut(s)	1436
MlyI	GAGTC	3 cut(s)	122, 524, 1871
MmeI	TCCRAC	5 cut(s)	272, 339, 1028, 2082, 2087
Mox20I	TGGCCA	1 cut(s)	1436
Mph1103I	ATGCAT	2 cut(s)	769, 1470
MscI	TGGCCA	1 cut(s)	1436
MseI	TTAA	8 cut(s)	522, 666, 729, 888, 942, 1257, 1580, 1638
MslI	CAYNNNNRTG	1 cut(s)	1074
Msp20I	TGGCCA	1 cut(s)	1436
MspA1I	CMGCKG	2 cut(s)	1204, 1678
MspI	CCGG	7 cut(s)	440, 451, 481, 722, 979, 1210, 1842
MspR9I	CCNGG	2 cut(s)	482, 1990
Mva1269I	GAATGC	1 cut(s)	743
MvaI	CCWGG	1 cut(s)	1990
MvnI	CGCG	4 cut(s)	356, 1671, 1858, 2081
MwoI	GCNNNNNNNGC	6 cut(s)	74, 275, 353, 386, 1792, 2078
NciI	CCSGG	1 cut(s)	482
NdeI	CATATG	1 cut(s)	2164
NdeII	GATC	7 cut(s)	87, 394, 1175, 1355, 1504, 1536, 1619
NheI	GCTAGC	1 cut(s)	1831
NlaIII	CATG	6 cut(s)	460, 586, 1015, 1170, 1736, 2297
NlaIV	GGNNCC	1 cut(s)	449
NmuCI	GTSAC	1 cut(s)	2033
NruI	TCGCGA	2 cut(s)	1671, 2081
NsbI	TGCGCA	1 cut(s)	1485
NsiI	ATGCAT	2 cut(s)	769, 1470
NspI	RCATGY	1 cut(s)	586
PciI	ACATGT	1 cut(s)	582
PciSI	GCTCTTC	2 cut(s)	197, 347
PcsI	WCGNNNNNNNCGW	3 cut(s)	201, 315, 414
PctI	GAATGC	1 cut(s)	743
PfeI	GAWTC	3 cut(s)	802, 1666, 2308
Pfl23II	CGTACG	1 cut(s)	15
PflFI	GACNNNGTC	1 cut(s)	302
PflMI	CCANNNNNTGG	1 cut(s)	291
PinAI	ACCGGT	1 cut(s)	1209
PkrI	GCNGC	5 cut(s)	271, 1203, 1389, 1680, 2147
PleI	GAGTC	3 cut(s)	121, 524, 1871
PpsI	GAGTC	3 cut(s)	121, 524, 1871
Ppu21I	YACGTR	3 cut(s)	418, 1429, 1814
PscI	ACATGT	1 cut(s)	582
Psp6I	CCWGG	1 cut(s)	1988
PspGI	CCWGG	1 cut(s)	1988
PspLI	CGTACG	1 cut(s)	15
PspN4I	GGNNCC	1 cut(s)	449
PspPI	GGNCC	4 cut(s)	448, 719, 1573, 2204
PstI	CTGCAG	2 cut(s)	1389, 1683
PstNI	CAGNNNCTG	1 cut(s)	1823
PsuI	RGATCY	3 cut(s)	87, 1175, 1536
PsyI	GACNNNGTC	1 cut(s)	302
PvuII	CAGCTG	1 cut(s)	1678
RruI	TCGCGA	2 cut(s)	1671, 2081
RsaI	GTAC	7 cut(s)	17, 1171, 1208, 1448, 1855, 1929, 2396
RsaNI	GTAC	7 cut(s)	16, 1170, 1207, 1447, 1854, 1928, 2395
RseI	CAYNNNNRTG	1 cut(s)	1074
SapI	GCTCTTC	2 cut(s)	197, 347
SaqAI	TTAA	8 cut(s)	522, 666, 729, 888, 942, 1257, 1580, 1638
SatI	GCNGC	5 cut(s)	270, 1202, 1388, 1679, 2146
Sau3AI	GATC	7 cut(s)	87, 394, 1175, 1355, 1504, 1536, 1619
Sau96I	GGNCC	4 cut(s)	448, 719, 1573, 2204
SchI	GAGTC	3 cut(s)	122, 524, 1871
ScrFI	CCNGG	2 cut(s)	482, 1990
SfaNI	GCATC	7 cut(s)	196, 320, 1120, 1330, 1623, 1702, 1963
SfcI	CTRYAG	3 cut(s)	1385, 1450, 1679
SinI	GGWCC	3 cut(s)	448, 1573, 2204
SmiMI	CAYNNNNRTG	1 cut(s)	1074
SspI	AATATT	2 cut(s)	578, 1980
SspMI	CTAG	2 cut(s)	711, 1832
StyD4I	CCNGG	2 cut(s)	480, 1988
StyI	CCWWGG	2 cut(s)	1304, 1458
TaaI	ACNGT	7 cut(s)	290, 304, 435, 1454, 1791, 1921, 1932
TaiI	ACGT	4 cut(s)	215, 420, 1431, 1816
TaqI	TCGA	7 cut(s)	111, 201, 613, 644, 1253, 1631, 2177
TaqII	GACCGA	1 cut(s)	2221
TatI	WGTACW	2 cut(s)	1169, 1446
TauI	GCSGC	2 cut(s)	272, 1204
TfiI	GAWTC	3 cut(s)	802, 1666, 2308
Tru1I	TTAA	8 cut(s)	522, 666, 729, 888, 942, 1257, 1580, 1638
Tru9I	TTAA	8 cut(s)	522, 666, 729, 888, 942, 1257, 1580, 1638
TscAI	CASTG	3 cut(s)	1248, 1771, 1874
TseFI	GTSAC	1 cut(s)	2033
TseI	GCWGC	3 cut(s)	1387, 1678, 2145
Tsp45I	GTSAC	1 cut(s)	2033
TspDTI	ATGAA	9 cut(s)	445, 675, 1113, 1462, 1487, 1592, 1795, 2057, 2234
TspGWI	ACGGA	1 cut(s)	1368
TspRI	CASTG	3 cut(s)	1248, 1771, 1874
Tth111I	GACNNNGTC	1 cut(s)	302
Van91I	CCANNNNNTGG	1 cut(s)	291
VpaK11BI	GGWCC	3 cut(s)	448, 1573, 2204
XapI	RAATTY	2 cut(s)	668, 901
XceI	RCATGY	1 cut(s)	586
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1567
XmiI	GTMKAC	1 cut(s)	1875
XspI	CTAG	2 cut(s)	711, 1832
Zsp2I	ATGCAT	2 cut(s)	769, 1470

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.