Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr04

Orthogroup ID

OG0002852

Physical Location & Seq

Genomic Coordinates Reverse (-)

9304778 .. 9308205

Gene Structure 3428 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD04G1068700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1563 bp

ATGGACACAGCCAACAACAATGAGACTAGCCAGCCTTCGATCCAATTCTGCAATTCTTACAGAGTGGCAAGCCTTTTCGACACAATTCTGGAAGCAACCCAAGTGGCAGATTTGAAAGACCGGTATATTCTAGGTGAAGAATTGGGATGGGGACAGTTTGGTGTCATTAGGGCATGCTCGGATAAGTTTACTGGTGAGGTTTTAGCTTGCAAGTCTATCGCCAAAGATAGATTGGCCACGCACAATGATGTGCGCAGCATCAAGCTTGAGATTGAAATCTTGTGTAGGTTGTCTGGGCACCCGAATGTTGTAAATTTAAAGGCAGTTTATGAGGAGGAAAATCATGTGCATTTGTTGATGGAGTTATGTGCTGGAGGGGAGCTTTTTCATCGGCTTGAGAAGCAGGGAAGATTCTCAGAAACTGATGCTCGGGTTCTTTTTAGACATCTGATGAAAGTTGTTAAGTATTGTCATGAGAACGGCGTTGTTCACAGAGATTTGAAGCCGGAAAACATTCTCTTGGCTACAACTTCTTCATCCTCACCAATTAAGTTGGCAGACTTTGGCCTTGCAACCTACATCAAACCCGGGCAGAAACTGCATGGGACTGTTGGCAGTCCATTTTACATAGCTCCAGAAGTACTTGCAGGAGGTTATAACCAGTCTGCTGATGTATGGAGCGCTGGAGTTATTTTATACATACTTCTTAGTGGGGTGCCTCCTTTCTGGGGGAAGACTAAGTCAAGAATTTTTGATGCTGTAAGGGTTGCAGATCTGTGGTTCCCTCCCGATCCTTGGGGTCACGTATCTGCATCTGCCAAGGAATTGATCGCTGGAATGCTTTGCGTTGATCCTTCCAGAAGGCTCACAGCTGCACAGGTTCTAGCTCATCCATGGATGGAAGATCGTGCATATGCAAGTCAAGAGACACGCAAGCTGGACAATCAATTTCACAAACAGCTGGAAGTGGGTGGAGGTTCTTTCTCTACACCATTTATCTATACGAATCAGAACTTTAGCTTAGATGATGGCTCACCCACAGCTGGTGATGTGGAACTGTGGCAATCACCTGCATTCACATGCAAATCATCTTCGTCGGGTTTTCTAGTTGACAACGGTAGTCTTTGCCCTGCATCTGGAGGTTTTTCTTTTAGCAGCTGTTATGAATTAAGTCCGCTGGAGTTTTCTTCACCAGTTCCATCGATGCCTAGCTTTACCTTCTTCAGTCCAAGTTCTGCGGTAGAGCAAAGAAACATCTCGTTAAGATTTAAAACCAACATGGCGACATTAGATACGGCGTATTCAAGTGTGGAGAAGCTAATTGTGCTGACAGATTGCTCAGCTGTCAAGTGTGACGGTGGAGAAGTAGAGCGCAAAGAATTTTGGAGAGGCGGAACAAACGGGCCTTCTAGGGTTGCTGGAATCCACAGCAAGAGGAACAACACGATTGGACTTGGCGAGCTTGATCAGCTTAATCTGGTTGTAGCCGAATCAGTCATACGTTGGGCGTCATGCACGCATATCCCTACTGTCCCATCGCTTAGATTGTCGCTCGTCTGCTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001101 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004683 GO:0005488 GO:0005515 GO:0005516 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0009719 GO:0009725 GO:0009737 GO:0009738 GO:0009755 GO:0009931 GO:0009987 GO:0010033 GO:0010857 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0018105 GO:0018193 GO:0018209 GO:0019538 GO:0023052 GO:0032870 GO:0033993 GO:0035556 GO:0036211 GO:0042221 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0046777 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0065007 GO:0070887 GO:0071215 GO:0071229 GO:0071310 GO:0071396 GO:0071495 GO:0071704 GO:0071944 GO:0097305 GO:0097306 GO:0140096 GO:1901564 GO:1901700 GO:1901701

KEGG Pathways

Metabolic & Signaling

ko04012 ko04020 ko04024 ko04066 ko04114 ko04217 ko04261 ko04310 ko04360 ko04713 ko04720 ko04722 ko04725 ko04728 ko04740 ko04745 ko04750 ko04911 ko04912 ko04916 ko04921 ko04922 ko04925 ko04934 ko04971 ko05031 ko05152 ko05200 ko05205 ko05214 map04012 map04020 map04024 map04066 map04114 map04217 map04261 map04310 map04360 map04713 map04720 map04722 map04725 map04728 map04740 map04745 map04750 map04911 map04912 map04916 map04921 map04922 map04925 map04934 map04971 map05031 map05152 map05200 map05205 map05214

Pfam Domains

Protein Families

Pkinase

Protein Analysis

521

Amino Acids

57.12

Weight (kDa)

6.08

Isoelectric Point (pI)

45.84

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pkinase	PF00069	43 - 300	4e-73	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	44 - 294	2.2e-30	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002852)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G12580
fragaria_vesca	FvH4_5g01410 FvH4_5g01410 FvH4_5g01410 FvH4_7g28910 FvH4_7g28910 FvH4_7g28910
malus_domestica	MD01G1195700.v1.1 MD04G1068700.v1.1 MD07G1262500.v1.1
prunus_persica	Prupe.2G289000_v2.0.a1 Prupe.5G089500_v2.0.a1 Prupe.5G089500_v2.0.a1
pyrus_communis	pycom01g20630 pycom04g06200 pycom07g24150
rosa_chinensis	RchiOBHm_Chr1g0376571 RchiOBHm_Chr7g0199461
rosa_laevigata	RLG00000003791 RLG00000026597
rosa_multiflora	Rmu_sc0001308.1_g000003 Rmu_sc0003890.1_g000006 Rmu_sc0005594.1_g000017
rosa_roxburghii	Rroxscaffold_3G00256710 Rroxscaffold_4G00281980
rosa_rugosa	Rorug01G0396500 Rorug07G0054100 Rorug07G0054200 Rorug07G0054200 Rorug07G0054300
rosa_samantha	Rh1AG412300 Rh1BG372700 Rh1CG386600 Rh1DG403600 Rh7AG179200 Rh7BG182600 Rh7DG182100
rosa_wichuraiana	Rw1G036210 Rw7G015740

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	657
AarI	CACCTGC	1 cut(s)	1078
Acc16I	TGCGCA	1 cut(s)	254
Acc36I	ACCTGC	1 cut(s)	1078
AccB1I	GGYRCC	2 cut(s)	297, 715
AciI	CCGC	3 cut(s)	1175, 1238, 1392
AclWI	GGATC	3 cut(s)	34, 785, 845
AcoI	YGGCCR	1 cut(s)	234
AcsI	RAATTY	3 cut(s)	313, 747, 1379
AcuI	CTGAAG	1 cut(s)	1207
AcyI	GRCGYC	1 cut(s)	1508
AfaI	GTAC	1 cut(s)	642
AfeI	AGCGCT	1 cut(s)	682
AfiI	CCNNNNNNNGG	4 cut(s)	728, 795, 1043, 1136
AgeI	ACCGGT	1 cut(s)	120
AgsI	TTSAA	4 cut(s)	115, 275, 502, 1305
Alw26I	GTCTC	2 cut(s)	17, 920
AlwI	GGATC	3 cut(s)	34, 785, 845
AlwNI	CAGNNNCTG	2 cut(s)	422, 598
Ama87I	CYCGRG	2 cut(s)	429, 587
Aor51HI	AGCGCT	1 cut(s)	682
AoxI	GGCC	3 cut(s)	234, 565, 1403
ApeKI	GCWGC	3 cut(s)	255, 872, 1155
ApoI	RAATTY	3 cut(s)	313, 747, 1379
AsiGI	ACCGGT	1 cut(s)	120
Asp700I	GAANNNNTTC	1 cut(s)	513
AspLEI	GCGC	3 cut(s)	255, 683, 1374
AspS9I	GGNCC	1 cut(s)	1403
AsuC2I	CCSGG	2 cut(s)	588, 589
AsuHPI	GGTGA	7 cut(s)	146, 206, 534, 1026, 1058, 1059, 1182
AvaI	CYCGRG	2 cut(s)	429, 587
BaeGI	GKGCMC	1 cut(s)	300
BalI	TGGCCA	1 cut(s)	236
BanI	GGYRCC	2 cut(s)	297, 715
BbsI	GAAGAC	1 cut(s)	740
BbvI	GCAGC	3 cut(s)	267, 859, 1167
BccI	CCATC	6 cut(s)	141, 352, 892, 1022, 1207, 1543
BceAI	ACGGC	2 cut(s)	496, 1311
BcgI	CGANNNNNNTGC	2 cut(s)	199, 233
BclI	TGATCA	1 cut(s)	1465
BcnI	CCSGG	2 cut(s)	588, 589
BcoDI	GTCTC	2 cut(s)	17, 920
BfaI	CTAG	7 cut(s)	27, 131, 884, 1106, 1209, 1410, 1561
BfoI	RGCGCY	1 cut(s)	684
BfuAI	ACCTGC	1 cut(s)	1078
BglII	AGATCT	1 cut(s)	772
BisI	GCNGC	3 cut(s)	256, 873, 1156
BlpI	GCTNAGC	1 cut(s)	1339
BlsI	GCNGC	3 cut(s)	257, 874, 1157
BmcAI	AGTACT	1 cut(s)	642
Bme1390I	CCNGG	2 cut(s)	588, 589
BmeT110I	CYCGRG	2 cut(s)	429, 587
BmgT120I	GGNCC	1 cut(s)	1403
BmiI	GGNNCC	3 cut(s)	299, 717, 782
BmrFI	CCNGG	2 cut(s)	588, 589
BmsI	GCATC	6 cut(s)	267, 415, 745, 821, 1142, 1194
BpiI	GAAGAC	1 cut(s)	740
BpmI	CTGGAG	5 cut(s)	393, 618, 705, 1158, 1199
Bpu1102I	GCTNAGC	1 cut(s)	1339
BpuEI	CTTGAG	2 cut(s)	287, 416
BpuMI	CCSGG	2 cut(s)	588, 589
Bsa29I	ATCGAT	1 cut(s)	1202
BsaAI	YACGTR	1 cut(s)	805
BsaBI	GATNNNNATC	1 cut(s)	275
BsaHI	GRCGYC	1 cut(s)	1508
BsaJI	CCNNGG	4 cut(s)	587, 794, 819, 893
BsaWI	WCCGGW	1 cut(s)	120
Bsc4I	CCNNNNNNNGG	4 cut(s)	728, 795, 1043, 1136
Bse118I	RCCGGY	1 cut(s)	120
Bse1I	ACTGG	3 cut(s)	196, 661, 1193
Bse8I	GATNNNNATC	1 cut(s)	275
BseCI	ATCGAT	1 cut(s)	1202
BseDI	CCNNGG	4 cut(s)	587, 794, 819, 893
BseGI	GGATG	4 cut(s)	152, 536, 889, 903
BseJI	GATNNNNATC	1 cut(s)	275
BseLI	CCNNNNNNNGG	4 cut(s)	728, 795, 1043, 1136
BseMII	CTCAG	2 cut(s)	429, 1353
BseNI	ACTGG	3 cut(s)	196, 661, 1193
BseRI	GAGGAG	1 cut(s)	347
BseSI	GKGCMC	1 cut(s)	300
BseXI	GCAGC	3 cut(s)	267, 859, 1167
BsgI	GTGCAG	1 cut(s)	858
BshFI	GGCC	3 cut(s)	236, 567, 1405
BshNI	GGYRCC	2 cut(s)	297, 715
BshTI	ACCGGT	1 cut(s)	120
BshVI	ATCGAT	1 cut(s)	1202
BsiHKCI	CYCGRG	2 cut(s)	429, 587
BsiSI	CCGG	3 cut(s)	121, 506, 588
BslFI	GGGAC	3 cut(s)	165, 619, 1517
BslI	CCNNNNNNNGG	4 cut(s)	728, 795, 1043, 1136
BsmAI	GTCTC	2 cut(s)	17, 920
BsmFI	GGGAC	3 cut(s)	165, 619, 1517
BsmI	GAATGC	2 cut(s)	843, 1073
BsnI	GGCC	3 cut(s)	236, 567, 1405
BsoBI	CYCGRG	2 cut(s)	429, 587
Bsp1286I	GDGCHC	1 cut(s)	300
Bsp143I	GATC	7 cut(s)	39, 772, 790, 828, 850, 904, 1465
Bsp1720I	GCTNAGC	1 cut(s)	1339
Bsp19I	CCATGG	1 cut(s)	893
BspACI	CCGC	3 cut(s)	1175, 1238, 1392
BspANI	GGCC	3 cut(s)	236, 567, 1405
BspCNI	CTCAG	2 cut(s)	428, 1352
BspDI	ATCGAT	1 cut(s)	1202
BspHI	TCATGA	1 cut(s)	472
BspLI	GGNNCC	3 cut(s)	299, 717, 782
BspMI	ACCTGC	1 cut(s)	1078
BspPI	GGATC	3 cut(s)	34, 785, 845
BspT107I	GGYRCC	2 cut(s)	297, 715
BsrFI	RCCGGY	1 cut(s)	120
BsrI	ACTGG	3 cut(s)	196, 661, 1193
BssAI	RCCGGY	1 cut(s)	120
BssECI	CCNNGG	4 cut(s)	587, 794, 819, 893
BssMI	GATC	7 cut(s)	39, 772, 790, 828, 850, 904, 1465
BssNI	GRCGYC	1 cut(s)	1508
BssT1I	CCWWGG	3 cut(s)	794, 819, 893
Bst4CI	ACNGT	6 cut(s)	156, 610, 1059, 1118, 1358, 1531
BstACI	GRCGYC	1 cut(s)	1508
BstAPI	GCANNNNNTGC	1 cut(s)	598
BstBAI	YACGTR	1 cut(s)	805
BstC8I	GCNNGC	7 cut(s)	32, 70, 175, 208, 935, 1460, 1517
BstDEI	CTNAG	6 cut(s)	415, 707, 738, 1021, 1339, 1541
BstDSI	CCRYGG	1 cut(s)	893
BstF5I	GGATG	4 cut(s)	152, 536, 889, 903
BstH2I	RGCGCY	1 cut(s)	684
BstHHI	GCGC	3 cut(s)	255, 683, 1374
BstKTI	GATC	7 cut(s)	42, 775, 793, 831, 853, 907, 1468
BstMAI	GTCTC	2 cut(s)	17, 920
BstMBI	GATC	7 cut(s)	39, 772, 790, 828, 850, 904, 1465
BstMWI	GCNNNNNNNGC	4 cut(s)	400, 598, 1324, 1468
BstNSI	RCATGY	2 cut(s)	177, 1083
BstSCI	CCNGG	2 cut(s)	586, 587
BstSLI	GKGCMC	1 cut(s)	300
BstV1I	GCAGC	3 cut(s)	267, 859, 1167
BstV2I	GAAGAC	1 cut(s)	740
BstX2I	RGATCY	1 cut(s)	772
BstYI	RGATCY	1 cut(s)	772
Bsu15I	ATCGAT	1 cut(s)	1202
BsuRI	GGCC	3 cut(s)	236, 567, 1405
BsuTUI	ATCGAT	1 cut(s)	1202
BtgI	CCRYGG	1 cut(s)	893
BtgZI	GCGATG	1 cut(s)	1521
BtsCI	GGATG	4 cut(s)	152, 536, 889, 903
BveI	ACCTGC	1 cut(s)	1078
Cac8I	GCNNGC	7 cut(s)	32, 70, 175, 208, 935, 1460, 1517
CaiI	CAGNNNCTG	2 cut(s)	422, 598
CciI	TCATGA	1 cut(s)	472
CfoI	GCGC	3 cut(s)	255, 683, 1374
Cfr10I	RCCGGY	1 cut(s)	120
Cfr13I	GGNCC	1 cut(s)	1403
Cfr9I	CCCGGG	1 cut(s)	587
ClaI	ATCGAT	1 cut(s)	1202
CseI	GACGC	1 cut(s)	1497
Csp6I	GTAC	1 cut(s)	641
CspAI	ACCGGT	1 cut(s)	120
CspCI	CAANNNNNGTGG	2 cut(s)	84, 119
CviAII	CATG	8 cut(s)	174, 344, 473, 602, 894, 1080, 1279, 1512
CviQI	GTAC	1 cut(s)	641
DdeI	CTNAG	6 cut(s)	415, 707, 738, 1021, 1339, 1541
DpnI	GATC	7 cut(s)	41, 774, 792, 830, 852, 906, 1467
DpnII	GATC	7 cut(s)	39, 772, 790, 828, 850, 904, 1465
DraI	TTTAAA	2 cut(s)	318, 1270
EaeI	YGGCCR	1 cut(s)	234
EciI	GGCGGA	1 cut(s)	1407
Eco130I	CCWWGG	3 cut(s)	794, 819, 893
Eco47III	AGCGCT	1 cut(s)	682
Eco57I	CTGAAG	1 cut(s)	1207
Eco88I	CYCGRG	2 cut(s)	429, 587
EcoT14I	CCWWGG	3 cut(s)	794, 819, 893
ErhI	CCWWGG	3 cut(s)	794, 819, 893
FaeI	CATG	8 cut(s)	177, 347, 476, 605, 897, 1083, 1282, 1515
FaqI	GGGAC	3 cut(s)	165, 619, 1517
FatI	CATG	8 cut(s)	173, 343, 472, 601, 893, 1079, 1278, 1511
FauNDI	CATATG	1 cut(s)	913
FbaI	TGATCA	1 cut(s)	1465
Fnu4HI	GCNGC	3 cut(s)	256, 873, 1156
FokI	GGATG	4 cut(s)	159, 523, 876, 910
Fsp4HI	GCNGC	3 cut(s)	256, 873, 1156
FspBI	CTAG	7 cut(s)	27, 131, 884, 1106, 1209, 1410, 1561
FspI	TGCGCA	1 cut(s)	254
GlaI	GCGC	3 cut(s)	254, 682, 1373
GluI	GCNGC	3 cut(s)	256, 873, 1156
GsuI	CTGGAG	5 cut(s)	393, 618, 705, 1158, 1199
HaeII	RGCGCY	1 cut(s)	684
HaeIII	GGCC	3 cut(s)	236, 567, 1405
HapII	CCGG	3 cut(s)	121, 506, 588
HgaI	GACGC	1 cut(s)	1497
HhaI	GCGC	3 cut(s)	255, 683, 1374
Hin1I	GRCGYC	1 cut(s)	1508
Hin1II	CATG	8 cut(s)	177, 347, 476, 605, 897, 1083, 1282, 1515
Hin6I	GCGC	3 cut(s)	253, 681, 1372
HinP1I	GCGC	3 cut(s)	253, 681, 1372
HincII	GTYRAC	1 cut(s)	1111
HindII	GTYRAC	1 cut(s)	1111
HindIII	AAGCTT	1 cut(s)	263
HinfI	GANTC	4 cut(s)	411, 1006, 1422, 1490
HpaII	CCGG	3 cut(s)	121, 506, 588
HphI	GGTGA	7 cut(s)	146, 206, 534, 1026, 1058, 1059, 1182
Hpy166II	GTNNAC	3 cut(s)	189, 490, 1111
Hpy188I	TCNGA	4 cut(s)	181, 418, 450, 1011
Hpy188III	TCNNGA	8 cut(s)	89, 473, 635, 744, 788, 858, 923, 1137
Hpy8I	GTNNAC	3 cut(s)	189, 490, 1111
Hpy99I	CGWCG	1 cut(s)	1099
HpyAV	CCTTC	5 cut(s)	45, 855, 864, 1228, 1416
HpyCH4III	ACNGT	6 cut(s)	156, 610, 1059, 1118, 1358, 1531
HpyCH4IV	ACGT	2 cut(s)	804, 1501
HpyF10VI	GCNNNNNNNGC	4 cut(s)	400, 598, 1324, 1468
HpyF3I	CTNAG	6 cut(s)	415, 707, 738, 1021, 1339, 1541
HpySE526I	ACGT	2 cut(s)	804, 1501
Hsp92I	GRCGYC	1 cut(s)	1508
Hsp92II	CATG	8 cut(s)	177, 347, 476, 605, 897, 1083, 1282, 1515
HspAI	GCGC	3 cut(s)	253, 681, 1372
Ksp22I	TGATCA	1 cut(s)	1465
Kzo9I	GATC	7 cut(s)	39, 772, 790, 828, 850, 904, 1465
LmnI	GCTCC	3 cut(s)	379, 637, 678
Lsp1109I	GCAGC	3 cut(s)	267, 859, 1167
LweI	GCATC	6 cut(s)	267, 415, 745, 821, 1142, 1194
MaeI	CTAG	7 cut(s)	27, 131, 884, 1106, 1209, 1410, 1561
MaeII	ACGT	2 cut(s)	804, 1501
MaeIII	GTNAC	2 cut(s)	800, 1352
MalI	GATC	7 cut(s)	41, 774, 792, 830, 852, 906, 1467
MboI	GATC	7 cut(s)	39, 772, 790, 828, 850, 904, 1465
MboII	GAAGA	8 cut(s)	149, 420, 525, 745, 914, 1083, 1179, 1213
MflI	RGATCY	1 cut(s)	772
MhlI	GDGCHC	1 cut(s)	300
MlsI	TGGCCA	1 cut(s)	236
MluNI	TGGCCA	1 cut(s)	236
Mox20I	TGGCCA	1 cut(s)	236
MroXI	GAANNNNTTC	1 cut(s)	513
MscI	TGGCCA	1 cut(s)	236
MseI	TTAA	7 cut(s)	317, 462, 549, 1169, 1262, 1269, 1473
MslI	CAYNNNNRTG	3 cut(s)	246, 303, 1078
Msp20I	TGGCCA	1 cut(s)	236
MspA1I	CMGCKG	6 cut(s)	872, 961, 1043, 1158, 1177, 1343
MspI	CCGG	3 cut(s)	121, 506, 588
MspR9I	CCNGG	2 cut(s)	588, 589
Mva1269I	GAATGC	2 cut(s)	843, 1073
MwoI	GCNNNNNNNGC	4 cut(s)	400, 598, 1324, 1468
NciI	CCSGG	2 cut(s)	588, 589
NcoI	CCATGG	1 cut(s)	893
NdeI	CATATG	1 cut(s)	913
NdeII	GATC	7 cut(s)	39, 772, 790, 828, 850, 904, 1465
NlaIII	CATG	8 cut(s)	177, 347, 476, 605, 897, 1083, 1282, 1515
NlaIV	GGNNCC	3 cut(s)	299, 717, 782
NmuCI	GTSAC	2 cut(s)	800, 1352
NsbI	TGCGCA	1 cut(s)	254
NspI	RCATGY	2 cut(s)	177, 1083
PaeI	GCATGC	1 cut(s)	177
PagI	TCATGA	1 cut(s)	472
PaqCI	CACCTGC	1 cut(s)	1078
PctI	GAATGC	2 cut(s)	843, 1073
PdmI	GAANNNNTTC	1 cut(s)	513
PfeI	GAWTC	4 cut(s)	411, 1006, 1422, 1490
PflFI	GACNNNGTC	1 cut(s)	739
PinAI	ACCGGT	1 cut(s)	120
PkrI	GCNGC	3 cut(s)	257, 874, 1157
Ppu21I	YACGTR	1 cut(s)	805
PsiI	TTATAA	1 cut(s)	657
PspN4I	GGNNCC	3 cut(s)	299, 717, 782
PspPI	GGNCC	1 cut(s)	1403
PstNI	CAGNNNCTG	2 cut(s)	422, 598
PsuI	RGATCY	1 cut(s)	772
PsyI	GACNNNGTC	1 cut(s)	739
PvuII	CAGCTG	5 cut(s)	872, 961, 1043, 1158, 1343
RsaI	GTAC	1 cut(s)	642
RsaNI	GTAC	1 cut(s)	641
RseI	CAYNNNNRTG	3 cut(s)	246, 303, 1078
SaqAI	TTAA	7 cut(s)	317, 462, 549, 1169, 1262, 1269, 1473
SatI	GCNGC	3 cut(s)	256, 873, 1156
Sau3AI	GATC	7 cut(s)	39, 772, 790, 828, 850, 904, 1465
Sau96I	GGNCC	1 cut(s)	1403
ScaI	AGTACT	1 cut(s)	642
ScrFI	CCNGG	2 cut(s)	588, 589
SduI	GDGCHC	1 cut(s)	300
SfaNI	GCATC	6 cut(s)	267, 415, 745, 821, 1142, 1194
SmaI	CCCGGG	1 cut(s)	589
SmiMI	CAYNNNNRTG	3 cut(s)	246, 303, 1078
SmlI	CTYRAG	2 cut(s)	266, 395
SmoI	CTYRAG	2 cut(s)	266, 395
SphI	GCATGC	1 cut(s)	177
SsiI	CCGC	3 cut(s)	1175, 1238, 1392
SspMI	CTAG	7 cut(s)	27, 131, 884, 1106, 1209, 1410, 1561
StyD4I	CCNGG	2 cut(s)	586, 587
StyI	CCWWGG	3 cut(s)	794, 819, 893
TaaI	ACNGT	6 cut(s)	156, 610, 1059, 1118, 1358, 1531
TaiI	ACGT	2 cut(s)	807, 1504
TaqI	TCGA	3 cut(s)	38, 78, 1202
TatI	WGTACW	1 cut(s)	640
TfiI	GAWTC	4 cut(s)	411, 1006, 1422, 1490
Tru1I	TTAA	7 cut(s)	317, 462, 549, 1169, 1262, 1269, 1473
Tru9I	TTAA	7 cut(s)	317, 462, 549, 1169, 1262, 1269, 1473
TseFI	GTSAC	2 cut(s)	800, 1352
TseI	GCWGC	3 cut(s)	255, 872, 1155
Tsp45I	GTSAC	2 cut(s)	800, 1352
TspDTI	ATGAA	4 cut(s)	377, 467, 525, 1179
TspMI	CCCGGG	1 cut(s)	587
Tth111I	GACNNNGTC	1 cut(s)	739
XapI	RAATTY	3 cut(s)	313, 747, 1379
XceI	RCATGY	2 cut(s)	177, 1083
XcmI	CCANNNNNNNNNTGG	1 cut(s)	229
XmaI	CCCGGG	1 cut(s)	587
XmnI	GAANNNNTTC	1 cut(s)	513
XspI	CTAG	7 cut(s)	27, 131, 884, 1106, 1209, 1410, 1561
ZrmI	AGTACT	1 cut(s)	642

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.