Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr1B

Orthogroup ID

OG0002852

Physical Location & Seq

Genomic Coordinates Forward (+)

50416553 .. 50420539

Gene Structure 3987 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh1BG372700.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1551 bp

ATGGCTGTTGCCAAGAGCAGCAGCAACAGGGAACCATCAACATGGCTATGCAATTCTTACAAAGTCTCGAGCTTAACGTCCTCCATCTTGGATGCAAATGAGACCTCAAATCTAAAAGACCAGTATATTCTTGGAGAGCAATTAGGTTGGGGTCAATTTGGTGTTATTAGGGTGTGCACTGATAAGTTTACGGGAGAGGTTTTGGCATGCAAATCAATAGCTAAAGATCGATTAGTGACCTCAGATGATGTCAGTAGCATCAAGCTTGAGATTGAAATCATGACCAGGTTGTCTGGGCACCCAAATGTTGTAGATCTCAAGGCGGTGTATGAAGAGGAAGAGTACGTTCATTTGGTGATGGAGCTCTGTGCAGGAGGGGAGCTTTTCCACCAGTTAGAGAAGCACGGGCATTTCTCTGAATCTGATGCAAGGGTTCTCTTTAGGCATCTGATTCAGGTAGTTTTATACTGTCATGAAAATGGGGTTGTCCACAGAGATTTGAAGCCTGAGAATATTCTGTTGGCCACAAAAGCCTCCTCATCTCCTATAAAATTGGCAGACTTTGGTCTTGCAACTTACATCAAGCCTGGCCAGAGTTTGCACGGTTTGGTGGGAAGTCCATTTTATATAGCGCCTGAGGTACTTGCCGGGGGCTATAACCAAGCTGCTGATGTTTGGAGTGCAGGGGTAATTCTTTACATTCTTCTCAGCGGAATGCCACCATTTTGGGGGAAGACAAAGTCAAGCATATTTGATGCCGTTAGGACAGCTGATCTGAGGTTCCCATCTGATCCCTGGGATGGCATTACTGAATCAGCTAATGATTTGATTCGAGGAATGCTCTGTAAAGATCCTTCTAAAAGGCTCACCGCTCACCAGGTTTTAGAGCATTCCTGGATGAGGAGTACTGAATCCCTTTCTAAACAACCTAGTCAATGTAAAAATCTAGGTTATAAAGAATGTGATGTGGGCAGTAGCTCATTTACCACCTCATTTATGTCCAGGAGTCAGGACATCAGCTTTGGTACCGCATCACCTAGTATATGTGAAGCCCAATCCCCTACATTCACAAGCAGGTCATCTTTTTCTCCTTTCCTGGTAGAGCCATCTACTCCTTGCTTGGCGTCTGGTGGGTTGTCATTCCGTAGCAATGACGTCTCCAATTGCCTGGAATTCTCCTCCCCTATTTCCTCAATGCCAAGCTTTGCATTCTTCAGCCCCAGTTCTGTGGTTGAGGAACGTAGTTGTTCGGTGGAGTTCTCAGCCAGCATAGATGCAGTTCATCAAGAGACAAGCTCAGGGAAGTTGCTCCTATTATCAGATTCCCTTTCATTTAGGCTTGAAGCTAGAGGAATGATTAACAGACCAGCAGTAGCTAAAAGATCCGGGGTGACAACTGGGTCTAGGACATTGGGCATCCACAGCAAGAGGAACCGCACAATTGGACTCGGTGAGCACGAGCAACTAGATTTCATGGTCAACGAATCAATCATCCGCTGGTCATCATGCACACATCTTACCACTTCTCTACGATCGTCTCTCGTTTGCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001101 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004683 GO:0005488 GO:0005515 GO:0005516 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0009719 GO:0009725 GO:0009737 GO:0009738 GO:0009755 GO:0009931 GO:0009987 GO:0010033 GO:0010857 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0018105 GO:0018193 GO:0018209 GO:0019538 GO:0023052 GO:0032870 GO:0033993 GO:0035556 GO:0036211 GO:0042221 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0046777 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0065007 GO:0070887 GO:0071215 GO:0071229 GO:0071310 GO:0071396 GO:0071495 GO:0071704 GO:0071944 GO:0097305 GO:0097306 GO:0140096 GO:1901564 GO:1901700 GO:1901701

KEGG Pathways

Metabolic & Signaling

ko04012 ko04020 ko04024 ko04066 ko04114 ko04217 ko04261 ko04310 ko04360 ko04713 ko04720 ko04722 ko04725 ko04728 ko04740 ko04745 ko04750 ko04911 ko04912 ko04916 ko04921 ko04922 ko04925 ko04934 ko04971 ko05031 ko05152 ko05200 ko05205 ko05214 map04012 map04020 map04024 map04066 map04114 map04217 map04261 map04310 map04360 map04713 map04720 map04722 map04725 map04728 map04740 map04745 map04750 map04911 map04912 map04916 map04921 map04922 map04925 map04934 map04971 map05031 map05152 map05200 map05205 map05214

Pfam Domains

Protein Families

Pkinase

Protein Analysis

516

Amino Acids

56.49

Weight (kDa)

6.12

Isoelectric Point (pI)

57.4

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pkinase	PF00069	43 - 300	3.6e-74	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	44 - 296	3.3e-31	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002852)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G12580
fragaria_vesca	FvH4_5g01410 FvH4_5g01410 FvH4_5g01410 FvH4_7g28910 FvH4_7g28910 FvH4_7g28910
malus_domestica	MD01G1195700.v1.1 MD04G1068700.v1.1 MD07G1262500.v1.1
prunus_persica	Prupe.2G289000_v2.0.a1 Prupe.5G089500_v2.0.a1 Prupe.5G089500_v2.0.a1
pyrus_communis	pycom01g20630 pycom04g06200 pycom07g24150
rosa_chinensis	RchiOBHm_Chr1g0376571 RchiOBHm_Chr7g0199461
rosa_laevigata	RLG00000003791 RLG00000026597
rosa_multiflora	Rmu_sc0001308.1_g000003 Rmu_sc0003890.1_g000006 Rmu_sc0005594.1_g000017
rosa_roxburghii	Rroxscaffold_3G00256710 Rroxscaffold_4G00281980
rosa_rugosa	Rorug01G0396500 Rorug07G0054100 Rorug07G0054200 Rorug07G0054200 Rorug07G0054300
rosa_samantha	Rh1AG412300 Rh1BG372700 Rh1CG386600 Rh1DG403600 Rh7AG179200 Rh7BG182600 Rh7DG182100
rosa_wichuraiana	Rw1G036210 Rw7G015740

Restriction Enzyme Sites

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Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	954
AasI	GACNNNNNNGTC	2 cut(s)	289, 1399
AatII	GACGTC	1 cut(s)	1158
Acc36I	ACCTGC	1 cut(s)	1065
Acc65I	GGTACC	1 cut(s)	1025
AccB1I	GGYRCC	2 cut(s)	297, 1025
AccBSI	CCGCTC	1 cut(s)	872
AciI	CCGC	6 cut(s)	323, 711, 870, 1029, 1435, 1495
AclWI	GGATC	3 cut(s)	785, 845, 1377
AcoI	YGGCCR	2 cut(s)	522, 589
AcsI	RAATTY	1 cut(s)	1172
AcuI	CTGAAG	1 cut(s)	1198
AcyI	GRCGYC	2 cut(s)	1124, 1155
AfaI	GTAC	4 cut(s)	344, 642, 907, 1027
AfiI	CCNNNNNNNGG	3 cut(s)	728, 800, 900
AgsI	TTSAA	3 cut(s)	275, 502, 1343
AjnI	CCWGG	8 cut(s)	284, 586, 794, 876, 893, 1001, 1095, 1167
AjuI	GAANNNNNNNTTGG	2 cut(s)	503, 535
Alw21I	GWGCWC	3 cut(s)	179, 366, 1458
Alw26I	GTCTC	5 cut(s)	70, 95, 1162, 1283, 1542
Alw44I	GTGCAC	1 cut(s)	175
AlwI	GGATC	3 cut(s)	785, 845, 1377
Ama87I	CYCGRG	1 cut(s)	67
AoxI	GGCC	2 cut(s)	522, 589
ApaLI	GTGCAC	1 cut(s)	175
ApeKI	GCWGC	3 cut(s)	18, 21, 665
ApoI	RAATTY	1 cut(s)	1172
ArsI	GACNNNNNNTTYG	2 cut(s)	1004, 1036
Asp718I	GGTACC	1 cut(s)	1025
AspLEI	GCGC	1 cut(s)	634
AsuC2I	CCSGG	2 cut(s)	649, 1387
AsuHPI	GGTGA	6 cut(s)	367, 859, 866, 1026, 1402, 1463
AvaI	CYCGRG	1 cut(s)	67
AxyI	CCTNAGG	1 cut(s)	636
BaeGI	GKGCMC	2 cut(s)	179, 300
BalI	TGGCCA	2 cut(s)	524, 591
BanI	GGYRCC	2 cut(s)	297, 1025
BanII	GRGCYC	1 cut(s)	366
BarI	GAAGNNNNNNTAC	2 cut(s)	838, 870
BauI	CACGAG	1 cut(s)	1457
BbsI	GAAGAC	1 cut(s)	740
Bbv12I	GWGCWC	3 cut(s)	179, 366, 1458
BbvI	GCAGC	3 cut(s)	30, 33, 652
BccI	CCATC	6 cut(s)	43, 92, 352, 793, 794, 1114
BceAI	ACGGC	1 cut(s)	743
BciT130I	CCWGG	8 cut(s)	286, 588, 796, 878, 895, 1003, 1097, 1169
BcnI	CCSGG	2 cut(s)	649, 1387
BcoDI	GTCTC	5 cut(s)	70, 95, 1162, 1283, 1542
BfaI	CTAG	6 cut(s)	930, 947, 1038, 1347, 1404, 1466
BfoI	RGCGCY	1 cut(s)	635
BfuAI	ACCTGC	1 cut(s)	1065
BglII	AGATCT	1 cut(s)	313
BisI	GCNGC	3 cut(s)	19, 22, 666
BlsI	GCNGC	3 cut(s)	20, 23, 667
BmcAI	AGTACT	1 cut(s)	907
BmeT110I	CYCGRG	1 cut(s)	67
BmiI	GGNNCC	5 cut(s)	33, 299, 782, 1027, 1433
BmrI	ACTGGG	2 cut(s)	1215, 1407
BmsI	GCATC	8 cut(s)	82, 267, 415, 454, 745, 1040, 1264, 1425
BmuI	ACTGGG	2 cut(s)	1215, 1407
BoxI	GACNNNNGTC	1 cut(s)	564
BpiI	GAAGAC	1 cut(s)	740
BplI	GAGNNNNNCTC	4 cut(s)	825, 857, 1280, 1312
Bpu10I	CCTNAGC	1 cut(s)	1297
BpuEI	CTTGAG	2 cut(s)	287, 302
BpuMI	CCSGG	2 cut(s)	649, 1387
Bsa29I	ATCGAT	1 cut(s)	229
BsaBI	GATNNNNATC	1 cut(s)	275
BsaHI	GRCGYC	2 cut(s)	1124, 1155
BsaI	GGTCTC	1 cut(s)	95
BsaJI	CCNNGG	4 cut(s)	648, 794, 795, 1386
Bsc4I	CCNNNNNNNGG	3 cut(s)	728, 800, 900
Bse1I	ACTGG	4 cut(s)	121, 391, 1221, 1402
Bse21I	CCTNAGG	1 cut(s)	636
Bse3DI	GCAATG	1 cut(s)	1156
Bse8I	GATNNNNATC	1 cut(s)	275
BseBI	CCWGG	8 cut(s)	286, 588, 796, 878, 895, 1003, 1097, 1169
BseCI	ATCGAT	1 cut(s)	229
BseDI	CCNNGG	4 cut(s)	648, 794, 795, 1386
BseGI	GGATG	5 cut(s)	97, 805, 903, 1416, 1491
BseJI	GATNNNNATC	1 cut(s)	275
BseLI	CCNNNNNNNGG	3 cut(s)	728, 800, 900
BseMI	GCAATG	1 cut(s)	1156
BseMII	CTCAG	7 cut(s)	255, 498, 627, 721, 767, 1275, 1311
BseNI	ACTGG	4 cut(s)	121, 391, 1221, 1402
BseRI	GAGGAG	3 cut(s)	526, 916, 1168
BseSI	GKGCMC	2 cut(s)	179, 300
BseXI	GCAGC	3 cut(s)	30, 33, 652
BsgI	GTGCAG	2 cut(s)	390, 702
Bsh1285I	CGRYCG	1 cut(s)	1535
BshFI	GGCC	2 cut(s)	524, 591
BshNI	GGYRCC	2 cut(s)	297, 1025
BshVI	ATCGAT	1 cut(s)	229
BsiEI	CGRYCG	1 cut(s)	1535
BsiHKAI	GWGCWC	3 cut(s)	179, 366, 1458
BsiHKCI	CYCGRG	1 cut(s)	67
BsiSI	CCGG	2 cut(s)	648, 1386
BslI	CCNNNNNNNGG	3 cut(s)	728, 800, 900
BsmAI	GTCTC	5 cut(s)	70, 95, 1162, 1283, 1542
BsmBI	CGTCTC	2 cut(s)	1162, 1542
BsmI	GAATGC	4 cut(s)	720, 843, 889, 1208
BsnI	GGCC	2 cut(s)	524, 591
Bso31I	GGTCTC	1 cut(s)	95
BsoBI	CYCGRG	1 cut(s)	67
Bsp1286I	GDGCHC	4 cut(s)	179, 300, 366, 1458
Bsp143I	GATC	7 cut(s)	226, 313, 772, 790, 850, 1382, 1532
BspACI	CCGC	6 cut(s)	323, 711, 870, 1029, 1435, 1495
BspANI	GGCC	2 cut(s)	524, 591
BspCNI	CTCAG	7 cut(s)	254, 499, 628, 720, 768, 1274, 1310
BspDI	ATCGAT	1 cut(s)	229
BspHI	TCATGA	2 cut(s)	279, 472
BspLI	GGNNCC	5 cut(s)	33, 299, 782, 1027, 1433
BspMI	ACCTGC	1 cut(s)	1065
BspPI	GGATC	3 cut(s)	785, 845, 1377
BspT107I	GGYRCC	2 cut(s)	297, 1025
BspTNI	GGTCTC	1 cut(s)	95
BsrBI	CCGCTC	1 cut(s)	872
BsrDI	GCAATG	1 cut(s)	1156
BsrI	ACTGG	4 cut(s)	121, 391, 1221, 1402
BssECI	CCNNGG	4 cut(s)	648, 794, 795, 1386
BssMI	GATC	7 cut(s)	226, 313, 772, 790, 850, 1382, 1532
BssNI	GRCGYC	2 cut(s)	1124, 1155
BssSI	CACGAG	1 cut(s)	1457
Bst2BI	CACGAG	1 cut(s)	1457
Bst2UI	CCWGG	8 cut(s)	286, 588, 796, 878, 895, 1003, 1097, 1169
Bst4CI	ACNGT	2 cut(s)	470, 605
Bst6I	CTCTTC	2 cut(s)	327, 333
BstACI	GRCGYC	2 cut(s)	1124, 1155
BstC8I	GCNNGC	2 cut(s)	208, 1267
BstDEI	CTNAG	7 cut(s)	241, 507, 636, 707, 776, 1261, 1297
BstENI	CCTNNNNNAGG	1 cut(s)	898
BstF5I	GGATG	5 cut(s)	97, 805, 903, 1416, 1491
BstH2I	RGCGCY	1 cut(s)	635
BstHHI	GCGC	1 cut(s)	634
BstKTI	GATC	7 cut(s)	229, 316, 775, 793, 853, 1385, 1535
BstMAI	GTCTC	5 cut(s)	70, 95, 1162, 1283, 1542
BstMBI	GATC	7 cut(s)	226, 313, 772, 790, 850, 1382, 1532
BstMCI	CGRYCG	1 cut(s)	1535
BstMWI	GCNNNNNNNGC	2 cut(s)	530, 1422
BstNI	CCWGG	8 cut(s)	286, 588, 796, 878, 895, 1003, 1097, 1169
BstNSI	RCATGY	1 cut(s)	210
BstPAI	GACNNNNGTC	1 cut(s)	564
BstSLI	GKGCMC	2 cut(s)	179, 300
BstV1I	GCAGC	3 cut(s)	30, 33, 652
BstV2I	GAAGAC	1 cut(s)	740
BstX2I	RGATCY	3 cut(s)	313, 850, 1382
BstXI	CCANNNNNNTGG	4 cut(s)	42, 726, 1168, 1228
BstYI	RGATCY	3 cut(s)	313, 850, 1382
Bsu15I	ATCGAT	1 cut(s)	229
Bsu36I	CCTNAGG	1 cut(s)	636
BsuRI	GGCC	2 cut(s)	524, 591
BsuTUI	ATCGAT	1 cut(s)	229
BtsCI	GGATG	5 cut(s)	97, 805, 903, 1416, 1491
BtsIMutI	CAGTG	1 cut(s)	177
BveI	ACCTGC	1 cut(s)	1065
Cac8I	GCNNGC	2 cut(s)	208, 1267
CciI	TCATGA	2 cut(s)	279, 472
CfoI	GCGC	1 cut(s)	634
ClaI	ATCGAT	1 cut(s)	229
CseI	GACGC	1 cut(s)	1113
CsiI	ACCWGGT	2 cut(s)	284, 876
Csp6I	GTAC	4 cut(s)	343, 641, 906, 1026
CviAII	CATG	6 cut(s)	42, 207, 280, 473, 1474, 1506
CviQI	GTAC	4 cut(s)	343, 641, 906, 1026
DdeI	CTNAG	7 cut(s)	241, 507, 636, 707, 776, 1261, 1297
DpnI	GATC	7 cut(s)	228, 315, 774, 792, 852, 1384, 1534
DpnII	GATC	7 cut(s)	226, 313, 772, 790, 850, 1382, 1532
DrdI	GACNNNNNNGTC	2 cut(s)	289, 1399
DseDI	GACNNNNNNGTC	2 cut(s)	289, 1399
EaeI	YGGCCR	2 cut(s)	522, 589
Eam1104I	CTCTTC	2 cut(s)	327, 333
EarI	CTCTTC	2 cut(s)	327, 333
Ecl136II	GAGCTC	1 cut(s)	364
Eco24I	GRGCYC	1 cut(s)	366
Eco31I	GGTCTC	1 cut(s)	95
Eco53kI	GAGCTC	1 cut(s)	364
Eco57I	CTGAAG	1 cut(s)	1198
Eco81I	CCTNAGG	1 cut(s)	636
Eco88I	CYCGRG	1 cut(s)	67
EcoICRI	GAGCTC	1 cut(s)	364
EcoNI	CCTNNNNNAGG	1 cut(s)	898
EcoRI	GAATTC	1 cut(s)	1172
EcoRII	CCWGG	8 cut(s)	284, 586, 794, 876, 893, 1001, 1095, 1167
EcoT38I	GRGCYC	1 cut(s)	366
Esp3I	CGTCTC	2 cut(s)	1162, 1542
FaeI	CATG	6 cut(s)	45, 210, 283, 476, 1477, 1509
FatI	CATG	6 cut(s)	41, 206, 279, 472, 1473, 1505
Fnu4HI	GCNGC	3 cut(s)	19, 22, 666
FokI	GGATG	5 cut(s)	104, 812, 910, 1403, 1478
FriOI	GRGCYC	1 cut(s)	366
Fsp4HI	GCNGC	3 cut(s)	19, 22, 666
FspBI	CTAG	6 cut(s)	930, 947, 1038, 1347, 1404, 1466
GlaI	GCGC	1 cut(s)	633
GluI	GCNGC	3 cut(s)	19, 22, 666
HaeII	RGCGCY	1 cut(s)	635
HaeIII	GGCC	2 cut(s)	524, 591
HapII	CCGG	2 cut(s)	648, 1386
HgaI	GACGC	1 cut(s)	1113
HhaI	GCGC	1 cut(s)	634
Hin1I	GRCGYC	2 cut(s)	1124, 1155
Hin1II	CATG	6 cut(s)	45, 210, 283, 476, 1477, 1509
Hin6I	GCGC	1 cut(s)	632
HinP1I	GCGC	1 cut(s)	632
HincII	GTYRAC	1 cut(s)	1480
HindII	GTYRAC	1 cut(s)	1480
HindIII	AAGCTT	2 cut(s)	263, 1201
HinfI	GANTC	9 cut(s)	419, 451, 812, 829, 911, 1006, 1322, 1446, 1484
HpaII	CCGG	2 cut(s)	648, 1386
HphI	GGTGA	6 cut(s)	367, 859, 866, 1026, 1402, 1463
Hpy166II	GTNNAC	4 cut(s)	177, 189, 490, 1480
Hpy188I	TCNGA	7 cut(s)	244, 418, 424, 450, 777, 790, 1321
Hpy188III	TCNNGA	5 cut(s)	67, 280, 473, 1010, 1286
Hpy8I	GTNNAC	4 cut(s)	177, 189, 490, 1480
HpyAV	CCTTC	1 cut(s)	864
HpyCH4III	ACNGT	2 cut(s)	470, 605
HpyCH4IV	ACGT	4 cut(s)	77, 345, 1155, 1240
HpyF10VI	GCNNNNNNNGC	2 cut(s)	530, 1422
HpyF3I	CTNAG	7 cut(s)	241, 507, 636, 707, 776, 1261, 1297
HpySE526I	ACGT	4 cut(s)	77, 345, 1155, 1240
Hsp92I	GRCGYC	2 cut(s)	1124, 1155
Hsp92II	CATG	6 cut(s)	45, 210, 283, 476, 1477, 1509
HspAI	GCGC	1 cut(s)	632
KpnI	GGTACC	1 cut(s)	1029
Kzo9I	GATC	7 cut(s)	226, 313, 772, 790, 850, 1382, 1532
LmnI	GCTCC	3 cut(s)	361, 379, 1314
Lsp1109I	GCAGC	3 cut(s)	30, 33, 652
LweI	GCATC	8 cut(s)	82, 267, 415, 454, 745, 1040, 1264, 1425
MabI	ACCWGGT	2 cut(s)	284, 876
MaeI	CTAG	6 cut(s)	930, 947, 1038, 1347, 1404, 1466
MaeII	ACGT	4 cut(s)	77, 345, 1155, 1240
MaeIII	GTNAC	2 cut(s)	235, 1390
MalI	GATC	7 cut(s)	228, 315, 774, 792, 852, 1384, 1534
MbiI	CCGCTC	1 cut(s)	872
MboI	GATC	7 cut(s)	226, 313, 772, 790, 850, 1382, 1532
MboII	GAAGA	5 cut(s)	344, 350, 695, 745, 1204
MfeI	CAATTG	2 cut(s)	1162, 1440
MflI	RGATCY	3 cut(s)	313, 850, 1382
MhlI	GDGCHC	4 cut(s)	179, 300, 366, 1458
MlsI	TGGCCA	2 cut(s)	524, 591
MluCI	AATT	8 cut(s)	52, 140, 155, 551, 690, 1162, 1172, 1440
MluNI	TGGCCA	2 cut(s)	524, 591
MlyI	GAGTC	2 cut(s)	1015, 1440
Mox20I	TGGCCA	2 cut(s)	524, 591
MscI	TGGCCA	2 cut(s)	524, 591
MseI	TTAA	2 cut(s)	74, 1359
MslI	CAYNNNNRTG	4 cut(s)	40, 46, 303, 477
Msp20I	TGGCCA	2 cut(s)	524, 591
MspA1I	CMGCKG	3 cut(s)	711, 770, 1497
MspI	CCGG	2 cut(s)	648, 1386
MunI	CAATTG	2 cut(s)	1162, 1440
Mva1269I	GAATGC	4 cut(s)	720, 843, 889, 1208
MvaI	CCWGG	8 cut(s)	286, 588, 796, 878, 895, 1003, 1097, 1169
MwoI	GCNNNNNNNGC	2 cut(s)	530, 1422
NciI	CCSGG	2 cut(s)	649, 1387
NdeII	GATC	7 cut(s)	226, 313, 772, 790, 850, 1382, 1532
NlaIII	CATG	6 cut(s)	45, 210, 283, 476, 1477, 1509
NlaIV	GGNNCC	5 cut(s)	33, 299, 782, 1027, 1433
NmuCI	GTSAC	2 cut(s)	235, 1390
NspI	RCATGY	1 cut(s)	210
PaeI	GCATGC	1 cut(s)	210
PaeR7I	CTCGAG	1 cut(s)	67
PagI	TCATGA	2 cut(s)	279, 472
PasI	CCCWGGG	1 cut(s)	795
PcsI	WCGNNNNNNNCGW	2 cut(s)	74, 1455
PctI	GAATGC	4 cut(s)	720, 843, 889, 1208
PfeI	GAWTC	7 cut(s)	419, 451, 812, 829, 911, 1322, 1484
PflFI	GACNNNGTC	1 cut(s)	739
PfoI	TCCNGGA	2 cut(s)	893, 1001
PkrI	GCNGC	3 cut(s)	20, 23, 667
Ple19I	CGATCG	1 cut(s)	1535
PleI	GAGTC	2 cut(s)	1014, 1440
PpsI	GAGTC	2 cut(s)	1014, 1440
PshAI	GACNNNNGTC	1 cut(s)	564
PsiI	TTATAA	1 cut(s)	954
Psp124BI	GAGCTC	1 cut(s)	366
Psp6I	CCWGG	8 cut(s)	284, 586, 794, 876, 893, 1001, 1095, 1167
PspGI	CCWGG	8 cut(s)	284, 586, 794, 876, 893, 1001, 1095, 1167
PspN4I	GGNNCC	5 cut(s)	33, 299, 782, 1027, 1433
PsuI	RGATCY	3 cut(s)	313, 850, 1382
PsyI	GACNNNGTC	1 cut(s)	739
PvuI	CGATCG	1 cut(s)	1535
PvuII	CAGCTG	1 cut(s)	770
RsaI	GTAC	4 cut(s)	344, 642, 907, 1027
RsaNI	GTAC	4 cut(s)	343, 641, 906, 1026
RseI	CAYNNNNRTG	4 cut(s)	40, 46, 303, 477
SacI	GAGCTC	1 cut(s)	366
SaqAI	TTAA	2 cut(s)	74, 1359
SatI	GCNGC	3 cut(s)	19, 22, 666
Sau3AI	GATC	7 cut(s)	226, 313, 772, 790, 850, 1382, 1532
ScaI	AGTACT	1 cut(s)	907
SchI	GAGTC	2 cut(s)	1015, 1440
SduI	GDGCHC	4 cut(s)	179, 300, 366, 1458
SexAI	ACCWGGT	2 cut(s)	284, 876
SfaNI	GCATC	8 cut(s)	82, 267, 415, 454, 745, 1040, 1264, 1425
Sfr274I	CTCGAG	1 cut(s)	67
SlaI	CTCGAG	1 cut(s)	67
SmiMI	CAYNNNNRTG	4 cut(s)	40, 46, 303, 477
SmlI	CTYRAG	3 cut(s)	67, 266, 317
SmoI	CTYRAG	3 cut(s)	67, 266, 317
SphI	GCATGC	1 cut(s)	210
Sse9I	AATT	8 cut(s)	52, 140, 155, 551, 690, 1162, 1172, 1440
SsiI	CCGC	6 cut(s)	323, 711, 870, 1029, 1435, 1495
SspI	AATATT	1 cut(s)	514
SspMI	CTAG	6 cut(s)	930, 947, 1038, 1347, 1404, 1466
SstI	GAGCTC	1 cut(s)	366
TaaI	ACNGT	2 cut(s)	470, 605
TaiI	ACGT	4 cut(s)	80, 348, 1158, 1243
TaqI	TCGA	3 cut(s)	68, 229, 832
TasI	AATT	8 cut(s)	52, 140, 155, 551, 690, 1162, 1172, 1440
TatI	WGTACW	1 cut(s)	905
TfiI	GAWTC	7 cut(s)	419, 451, 812, 829, 911, 1322, 1484
Tru1I	TTAA	2 cut(s)	74, 1359
Tru9I	TTAA	2 cut(s)	74, 1359
TscAI	CASTG	1 cut(s)	184
TseFI	GTSAC	2 cut(s)	235, 1390
TseI	GCWGC	3 cut(s)	18, 21, 665
Tsp45I	GTSAC	2 cut(s)	235, 1390
TspDTI	ATGAA	6 cut(s)	338, 345, 489, 1271, 1320, 1462
TspGWI	ACGGA	1 cut(s)	1133
TspRI	CASTG	1 cut(s)	184
Tth111I	GACNNNGTC	1 cut(s)	739
VneI	GTGCAC	1 cut(s)	175
XagI	CCTNNNNNAGG	1 cut(s)	898
XapI	RAATTY	1 cut(s)	1172
XceI	RCATGY	1 cut(s)	210
XcmI	CCANNNNNNNNNTGG	2 cut(s)	128, 792
XhoI	CTCGAG	1 cut(s)	67
XspI	CTAG	6 cut(s)	930, 947, 1038, 1347, 1404, 1466
ZraI	GACGTC	1 cut(s)	1156
ZrmI	AGTACT	1 cut(s)	907

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.