Prupe.2G289000_v2.0.a1

Calcium-dependent protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp02
OG0002852
Physical Location & Seq
Forward (+)
28373284 .. 28377952
4669 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.2G289000.1

Sequence Viewer

Length: 1530 bp
ATGGCTGTTGCCAAGAGCAACAGCAGCAGTGCACCATCAACATGCCCATGCAATTGTTACGAAGTCCAAAGCTTGACTTCCTCCGTTTTGGATGCAAACCAGACAGCAAATCTAAAAGATCGGTACATTCTTGGAGAGCAATTAGGTTGGGGGCAGTTTGGTGTTATTAGGGCGTGCACAGATAAGTTGACCGGAGAGGTGTTGGCATGCAAATCAATTGCTAAAGATCGATTAGTGACCTCAGATGATGTGCGAAGCATCAAGCTTGAGATTGAAATTATGACCAGGTTATCTGGACACCCAAATGTTGTAGATCTCAAGGCGGTGTATGAGGAGGAAGAGTATGTTCATTTGGTGATGGAGCTTTGTGCAGGAGGGGAGCTTTTCCACCAGTTAGAGAAGCATGGAAGATTCTCTGAGTCTGATGCCAGGGTTCTCTTTAGGCATCTGATTCAGGTAGTTTTATATTGTCATGAAAATGGGGTTGTTCATAGAGATTTGAAGCCAGAGAACATCCTATTGGCCACAAAAGCCTCCTCATCTCCAATTAAATTGGCAGATTTTGGTCTTGCAACGTACATCAAGCCTGGGGAAAGTTTGCATGGGTTAGTAGGCAGTCCATTTTATATAGCTCCTGAGGTACTTGCAGGGAGCTATAACCAGGCTGCGGATGTTTGGAGTGCAGGGGTTATTCTTTACATTCTTCTAAGTGGAATGCCACCATTTTGGGGAAAGACAAAATCACGGATATTTGATGCTGTTAGGACAGCTGATCTGAGGTTCCCATCTGATCGCTGGGATCGTATTACTGAATCAGCTAAGGATCTGATTAGAGGAATGCTCTCTACAGATGCTTCACGAAGGCTCACTGCTCAGCAGGTTTTAGATCATCCTTGGATGAGTAGTCAACTGAGTCAGTGTGAAAGTGTAGGCTGTGACAATGGTAGCTCATTCTCTACCTCATTTATGTCCAGGAGTCGGGACATCAGCTTTGGGACTGGATCACCTAGTATATGTGATGCCCAGTCACCTGCATTTACTTGCAGGTCATCTTTTTCTTCTTTCATGGCGGAACCATTTACTCCATGCATAGCATCCAGTGGGTTTTCATTCTGCGGCAATGGTGATTCTAATGGTTTGGAATTCTCCTCGCCTGTTCCCTCAATGCCAAGCTTTGCATTCTTCAGCCCTGGCTCTTTGGTTGAGGAAAGTAGTTGTGCGTTAGAGTTCTCAGCCAGCATTTCCAGAGTAGACGCAATTCATGGAGAGACAAATGCAGGGAAGCTGCTCTTATTGCCAAATTCCCTTTCATTTGGACTTGAAGCTAGAGAAATGGTTAACAAACCAGTGGAAGCTAAAAGGACAGGTGGAACAATTGGATCCAGGACATCAGCTATTCACAGCAAGAGGAACCGTACAATCGGACTTGGTGAGCGTGAACAACTAGATTTCATGGTGACCGAATCAATCATTCGTTGGTCGTCATGCACACATCTTCCTACTTCTCTTAGATCTTCTCTTGTCTGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001101 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004683 GO:0005488 GO:0005515 GO:0005516 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0009719 GO:0009725 GO:0009737 GO:0009738 GO:0009755 GO:0009931 GO:0009987 GO:0010033 GO:0010857 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0018105 GO:0018193 GO:0018209 GO:0019538 GO:0023052 GO:0032870 GO:0033993 GO:0035556 GO:0036211 GO:0042221 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0046777 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0065007 GO:0070887 GO:0071215 GO:0071229 GO:0071310 GO:0071396 GO:0071495 GO:0071704 GO:0071944 GO:0097305 GO:0097306 GO:0140096 GO:1901564 GO:1901700 GO:1901701
KEGG Pathways
Metabolic & Signaling
ko04012 ko04020 ko04024 ko04066 ko04114 ko04217 ko04261 ko04310 ko04360 ko04713 ko04720 ko04722 ko04725 ko04728 ko04740 ko04745 ko04750 ko04911 ko04912 ko04916 ko04921 ko04922 ko04925 ko04934 ko04971 ko05031 ko05152 ko05200 ko05205 ko05214 map04012 map04020 map04024 map04066 map04114 map04217 map04261 map04310 map04360 map04713 map04720 map04722 map04725 map04728 map04740 map04745 map04750 map04911 map04912 map04916 map04921 map04922 map04925 map04934 map04971 map05031 map05152 map05200 map05205 map05214
Pfam Domains
Protein Families
Pkinase

Protein Analysis

510

Amino Acids

55.42

Weight (kDa)

5.99

Isoelectric Point (pI)

55.2

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1039
Acc36I ACCTGC 3 cut(s) 868, 1035, 1039
AccI GTMKAC 1 cut(s) 1251
AciI CCGC 4 cut(s) 323, 668, 1070, 1116
AclWI GGATC 5 cut(s) 807, 831, 1009, 1374, 1387
AcoI YGGCCR 1 cut(s) 522
AcsI RAATTY 2 cut(s) 1142, 1300
AcuI CTGAAG 1 cut(s) 1168
AfaI GTAC 4 cut(s) 125, 578, 642, 1417
AfiI CCNNNNNNNGG 3 cut(s) 667, 728, 978
AgsI TTSAA 3 cut(s) 275, 502, 1322
AjnI CCWGG 7 cut(s) 284, 428, 586, 660, 971, 1189, 1382
AjuI GAANNNNNNNTTGG 4 cut(s) 503, 535, 1291, 1323
Alw21I GWGCWC 2 cut(s) 34, 179
Alw26I GTCTC 1 cut(s) 1262
Alw44I GTGCAC 2 cut(s) 30, 175
AlwI GGATC 5 cut(s) 807, 831, 1009, 1374, 1387
AoxI GGCC 1 cut(s) 522
ApaLI GTGCAC 2 cut(s) 30, 175
ApeKI GCWGC 3 cut(s) 24, 665, 1285
ApoI RAATTY 2 cut(s) 1142, 1300
ArsI GACNNNNNNTTYG 2 cut(s) 974, 1006
AsuHPI GGTGA 6 cut(s) 367, 996, 1020, 1136, 1442, 1468
AxyI CCTNAGG 1 cut(s) 636
BaeGI GKGCMC 2 cut(s) 34, 179
BalI TGGCCA 1 cut(s) 524
BamHI GGATCC 1 cut(s) 1379
Bbv12I GWGCWC 2 cut(s) 34, 179
BbvI GCAGC 3 cut(s) 36, 652, 1272
BccI CCATC 3 cut(s) 43, 352, 793
BciT130I CCWGG 7 cut(s) 286, 430, 588, 662, 973, 1191, 1384
BcoDI GTCTC 1 cut(s) 1262
BfaI CTAG 3 cut(s) 1008, 1326, 1445
BfmI CTRYAG 1 cut(s) 846
BfuAI ACCTGC 3 cut(s) 868, 1035, 1039
BglII AGATCT 2 cut(s) 313, 1511
BisI GCNGC 4 cut(s) 25, 666, 1117, 1286
BlpI GCTNAGC 1 cut(s) 873
BlsI GCNGC 4 cut(s) 26, 667, 1118, 1287
Bme1390I CCNGG 7 cut(s) 286, 430, 588, 662, 973, 1191, 1384
BmiI GGNNCC 4 cut(s) 782, 1074, 1381, 1412
BmrFI CCNGG 7 cut(s) 286, 430, 588, 662, 973, 1191, 1384
BmrI ACTGGG 1 cut(s) 1018
BmsI GCATC 8 cut(s) 82, 267, 415, 454, 745, 841, 1009, 1103
BmuI ACTGGG 1 cut(s) 1018
BplI GAGNNNNNCTC 2 cut(s) 825, 857
Bpu10I CCTNAGC 1 cut(s) 819
Bpu1102I GCTNAGC 1 cut(s) 873
BpuEI CTTGAG 2 cut(s) 287, 302
Bsa29I ATCGAT 1 cut(s) 229
BsaJI CCNNGG 4 cut(s) 429, 587, 893, 1189
BsaWI WCCGGW 1 cut(s) 191
BsaXI ACNNNNNCTCC 2 cut(s) 326, 356
Bsc4I CCNNNNNNNGG 3 cut(s) 667, 728, 978
Bse1I ACTGG 5 cut(s) 391, 1003, 1024, 1098, 1346
Bse21I CCTNAGG 1 cut(s) 636
Bse3DI GCAATG 1 cut(s) 1126
BseBI CCWGG 7 cut(s) 286, 430, 588, 662, 973, 1191, 1384
BseCI ATCGAT 1 cut(s) 229
BseDI CCNNGG 4 cut(s) 429, 587, 893, 1189
BseGI GGATG 6 cut(s) 97, 513, 676, 889, 903, 1094
BseLI CCNNNNNNNGG 3 cut(s) 667, 728, 978
BseMI GCAATG 1 cut(s) 1126
BseMII CTCAG 7 cut(s) 255, 408, 627, 767, 887, 902, 1245
BseNI ACTGG 5 cut(s) 391, 1003, 1024, 1098, 1346
BseRI GAGGAG 3 cut(s) 347, 526, 1138
BseSI GKGCMC 2 cut(s) 34, 179
BseXI GCAGC 3 cut(s) 36, 652, 1272
BseYI CCCAGC 1 cut(s) 795
BsgI GTGCAG 2 cut(s) 390, 702
BshFI GGCC 1 cut(s) 524
BshVI ATCGAT 1 cut(s) 229
BsiHKAI GWGCWC 2 cut(s) 34, 179
BsiSI CCGG 1 cut(s) 192
BslFI GGGAC 2 cut(s) 995, 1009
BslI CCNNNNNNNGG 3 cut(s) 667, 728, 978
BsmAI GTCTC 1 cut(s) 1262
BsmFI GGGAC 2 cut(s) 995, 1009
BsmI GAATGC 3 cut(s) 720, 843, 1178
BsnI GGCC 1 cut(s) 524
Bsp1286I GDGCHC 2 cut(s) 34, 179
Bsp1720I GCTNAGC 1 cut(s) 873
BspACI CCGC 4 cut(s) 323, 668, 1070, 1116
BspANI GGCC 1 cut(s) 524
BspCNI CTCAG 7 cut(s) 254, 409, 628, 768, 886, 903, 1244
BspDI ATCGAT 1 cut(s) 229
BspHI TCATGA 1 cut(s) 472
BspLI GGNNCC 4 cut(s) 782, 1074, 1381, 1412
BspMI ACCTGC 3 cut(s) 868, 1035, 1039
BspPI GGATC 5 cut(s) 807, 831, 1009, 1374, 1387
BsrDI GCAATG 1 cut(s) 1126
BsrI ACTGG 5 cut(s) 391, 1003, 1024, 1098, 1346
BssECI CCNNGG 4 cut(s) 429, 587, 893, 1189
BssT1I CCWWGG 1 cut(s) 893
Bst2UI CCWGG 7 cut(s) 286, 430, 588, 662, 973, 1191, 1384
Bst4CI ACNGT 1 cut(s) 1415
Bst6I CTCTTC 1 cut(s) 333
BstC8I GCNNGC 3 cut(s) 175, 208, 1237
BstEII GGTNACC 1 cut(s) 1456
BstF5I GGATG 6 cut(s) 97, 513, 676, 889, 903, 1094
BstMAI GTCTC 1 cut(s) 1262
BstMWI GCNNNNNNNGC 3 cut(s) 24, 530, 1294
BstNI CCWGG 7 cut(s) 286, 430, 588, 662, 973, 1191, 1384
BstNSI RCATGY 2 cut(s) 45, 210
BstPI GGTNACC 1 cut(s) 1456
BstSCI CCNGG 7 cut(s) 284, 428, 586, 660, 971, 1189, 1382
BstSFI CTRYAG 1 cut(s) 846
BstSLI GKGCMC 2 cut(s) 34, 179
BstV1I GCAGC 3 cut(s) 36, 652, 1272
BstX2I RGATCY 4 cut(s) 313, 823, 1379, 1511
BstXI CCANNNNNNTGG 1 cut(s) 726
BstYI RGATCY 4 cut(s) 313, 823, 1379, 1511
Bsu15I ATCGAT 1 cut(s) 229
Bsu36I CCTNAGG 1 cut(s) 636
BsuRI GGCC 1 cut(s) 524
BsuTUI ATCGAT 1 cut(s) 229
BtsCI GGATG 6 cut(s) 97, 513, 676, 889, 903, 1094
BtsI GCAGTG 2 cut(s) 34, 867
BtsIMutI CAGTG 5 cut(s) 34, 867, 923, 1105, 1353
BveI ACCTGC 3 cut(s) 868, 1035, 1039
Cac8I GCNNGC 3 cut(s) 175, 208, 1237
CciI TCATGA 1 cut(s) 472
ClaI ATCGAT 1 cut(s) 229
CseI GACGC 1 cut(s) 1262
CsiI ACCWGGT 1 cut(s) 284
Csp6I GTAC 4 cut(s) 124, 577, 641, 1416
CviQI GTAC 4 cut(s) 124, 577, 641, 1416
EaeI YGGCCR 1 cut(s) 522
Eam1104I CTCTTC 1 cut(s) 333
EarI CTCTTC 1 cut(s) 333
EciI GGCGGA 1 cut(s) 1085
Eco130I CCWWGG 1 cut(s) 893
Eco57I CTGAAG 1 cut(s) 1168
Eco81I CCTNAGG 1 cut(s) 636
Eco91I GGTNACC 1 cut(s) 1456
EcoO65I GGTNACC 1 cut(s) 1456
EcoRI GAATTC 1 cut(s) 1142
EcoRII CCWGG 7 cut(s) 284, 428, 586, 660, 971, 1189, 1382
EcoT14I CCWWGG 1 cut(s) 893
EcoT22I ATGCAT 1 cut(s) 1091
ErhI CCWWGG 1 cut(s) 893
FalI AAGNNNNNCTT 4 cut(s) 61, 93, 1274, 1306
FaqI GGGAC 2 cut(s) 995, 1009
FblI GTMKAC 1 cut(s) 1251
Fnu4HI GCNGC 4 cut(s) 25, 666, 1117, 1286
FokI GGATG 6 cut(s) 104, 500, 683, 876, 910, 1081
Fsp4HI GCNGC 4 cut(s) 25, 666, 1117, 1286
FspBI CTAG 3 cut(s) 1008, 1326, 1445
GluI GCNGC 4 cut(s) 25, 666, 1117, 1286
GsaI CCCAGC 1 cut(s) 799
HaeIII GGCC 1 cut(s) 524
HapII CCGG 1 cut(s) 192
HgaI GACGC 1 cut(s) 1262
HincII GTYRAC 3 cut(s) 189, 908, 1339
HindII GTYRAC 3 cut(s) 189, 908, 1339
HindIII AAGCTT 3 cut(s) 70, 263, 1171
HinfI GANTC 8 cut(s) 411, 419, 451, 812, 913, 976, 1127, 1463
HpaI GTTAAC 1 cut(s) 1339
HpaII CCGG 1 cut(s) 192
HphI GGTGA 6 cut(s) 367, 996, 1020, 1136, 1442, 1468
Hpy166II GTNNAC 7 cut(s) 32, 177, 189, 908, 1252, 1339, 1439
Hpy188I TCNGA 8 cut(s) 244, 418, 424, 450, 777, 790, 828, 1424
Hpy188III TCNNGA 6 cut(s) 294, 473, 635, 858, 980, 1245
Hpy8I GTNNAC 7 cut(s) 32, 177, 189, 908, 1252, 1339, 1439
HpyAV CCTTC 1 cut(s) 855
HpyCH4III ACNGT 1 cut(s) 1415
HpyCH4IV ACGT 1 cut(s) 575
HpyF10VI GCNNNNNNNGC 3 cut(s) 24, 530, 1294
HpySE526I ACGT 1 cut(s) 575
KspAI GTTAAC 1 cut(s) 1339
LmnI GCTCC 4 cut(s) 361, 379, 637, 651
Lsp1109I GCAGC 3 cut(s) 36, 652, 1272
LweI GCATC 8 cut(s) 82, 267, 415, 454, 745, 841, 1009, 1103
MabI ACCWGGT 1 cut(s) 284
MaeI CTAG 3 cut(s) 1008, 1326, 1445
MaeII ACGT 1 cut(s) 575
MaeIII GTNAC 5 cut(s) 56, 235, 935, 1026, 1456
MboII GAAGA 7 cut(s) 350, 420, 695, 1050, 1174, 1487, 1506
MfeI CAATTG 3 cut(s) 52, 216, 1374
MflI RGATCY 4 cut(s) 313, 823, 1379, 1511
MhlI GDGCHC 2 cut(s) 34, 179
MlsI TGGCCA 1 cut(s) 524
MluNI TGGCCA 1 cut(s) 524
MlyI GAGTC 3 cut(s) 428, 922, 985
Mox20I TGGCCA 1 cut(s) 524
Mph1103I ATGCAT 1 cut(s) 1091
MscI TGGCCA 1 cut(s) 524
MseI TTAA 3 cut(s) 549, 1338, 1528
MslI CAYNNNNRTG 4 cut(s) 40, 46, 303, 477
Msp20I TGGCCA 1 cut(s) 524
MspA1I CMGCKG 1 cut(s) 770
MspI CCGG 1 cut(s) 192
MspR9I CCNGG 7 cut(s) 286, 430, 588, 662, 973, 1191, 1384
MunI CAATTG 3 cut(s) 52, 216, 1374
Mva1269I GAATGC 3 cut(s) 720, 843, 1178
MvaI CCWGG 7 cut(s) 286, 430, 588, 662, 973, 1191, 1384
MwoI GCNNNNNNNGC 3 cut(s) 24, 530, 1294
NlaIV GGNNCC 4 cut(s) 782, 1074, 1381, 1412
NmuCI GTSAC 4 cut(s) 235, 935, 1026, 1456
NsiI ATGCAT 1 cut(s) 1091
NspI RCATGY 2 cut(s) 45, 210
PaeI GCATGC 1 cut(s) 210
PagI TCATGA 1 cut(s) 472
PaqCI CACCTGC 1 cut(s) 1039
PcsI WCGNNNNNNNCGW 1 cut(s) 799
PctI GAATGC 3 cut(s) 720, 843, 1178
PfeI GAWTC 5 cut(s) 411, 451, 812, 1127, 1463
PfoI TCCNGGA 2 cut(s) 971, 1382
PkrI GCNGC 4 cut(s) 26, 667, 1118, 1287
PleI GAGTC 3 cut(s) 427, 921, 984
PpsI GAGTC 3 cut(s) 427, 921, 984
Psp6I CCWGG 7 cut(s) 284, 428, 586, 660, 971, 1189, 1382
PspEI GGTNACC 1 cut(s) 1456
PspFI CCCAGC 1 cut(s) 795
PspGI CCWGG 7 cut(s) 284, 428, 586, 660, 971, 1189, 1382
PspN4I GGNNCC 4 cut(s) 782, 1074, 1381, 1412
PsuI RGATCY 4 cut(s) 313, 823, 1379, 1511
PvuII CAGCTG 1 cut(s) 770
RsaI GTAC 4 cut(s) 125, 578, 642, 1417
RsaNI GTAC 4 cut(s) 124, 577, 641, 1416
RseI CAYNNNNRTG 4 cut(s) 40, 46, 303, 477
SaqAI TTAA 3 cut(s) 549, 1338, 1528
SatI GCNGC 4 cut(s) 25, 666, 1117, 1286
SchI GAGTC 3 cut(s) 428, 922, 985
ScrFI CCNGG 7 cut(s) 286, 430, 588, 662, 973, 1191, 1384
SduI GDGCHC 2 cut(s) 34, 179
SexAI ACCWGGT 1 cut(s) 284
SfaNI GCATC 8 cut(s) 82, 267, 415, 454, 745, 841, 1009, 1103
SfcI CTRYAG 1 cut(s) 846
SmiMI CAYNNNNRTG 4 cut(s) 40, 46, 303, 477
SmlI CTYRAG 2 cut(s) 266, 317
SmoI CTYRAG 2 cut(s) 266, 317
SphI GCATGC 1 cut(s) 210
SsiI CCGC 4 cut(s) 323, 668, 1070, 1116
SspMI CTAG 3 cut(s) 1008, 1326, 1445
StyD4I CCNGG 7 cut(s) 284, 428, 586, 660, 971, 1189, 1382
StyI CCWWGG 1 cut(s) 893
TaaI ACNGT 1 cut(s) 1415
TaiI ACGT 1 cut(s) 578
TaqI TCGA 1 cut(s) 229
TaqII GACCGA 1 cut(s) 1475
TauI GCSGC 1 cut(s) 1119
TfiI GAWTC 5 cut(s) 411, 451, 812, 1127, 1463
Tru1I TTAA 3 cut(s) 549, 1338, 1528
Tru9I TTAA 3 cut(s) 549, 1338, 1528
TscAI CASTG 5 cut(s) 34, 874, 923, 1105, 1353
TseFI GTSAC 4 cut(s) 235, 935, 1026, 1456
TseI GCWGC 3 cut(s) 24, 665, 1285
Tsp45I GTSAC 4 cut(s) 235, 935, 1026, 1456
TspDTI ATGAA 8 cut(s) 338, 479, 489, 1054, 1098, 1250, 1299, 1441
TspGWI ACGGA 2 cut(s) 73, 760
TspRI CASTG 5 cut(s) 34, 874, 923, 1105, 1353
VneI GTGCAC 2 cut(s) 30, 175
XapI RAATTY 2 cut(s) 1142, 1300
XceI RCATGY 2 cut(s) 45, 210
XcmI CCANNNNNNNNNTGG 1 cut(s) 792
XmiI GTMKAC 1 cut(s) 1251
XspI CTAG 3 cut(s) 1008, 1326, 1445
Zsp2I ATGCAT 1 cut(s) 1091
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.