Rorug07G0054200

Calcium-dependent protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000007
OG0002852
Physical Location & Seq
Reverse (-)
3840388 .. 3846738
6351 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug07G0054200.1

Sequence Viewer

Length: 4344 bp
ATGTATGGCCAAGCTAATTATGGTGCGCCGTTTGGCCAGGGTCAGCCACCTGCAAACCAACAGGGTCCGCCTCCACCTTCCCTTCAGTTCCAACAAGGCTCTCCAGCAGCACCACCGAATGTAGTTCAGATAGGTCAGCCGGGTCCTTATTTATATCAGCACGGTTCTGTTGCCCCACCTCCCGCTGCTCCGTTCAGTGGGATGCCAAATACAGGTCAATCTTACAGACACGCCAATCCAACAGTCCATGGAAGCACATGGCATAACGTGCAACATGCTTCAGCAACAGGAAACCAGAATTTCCAACCGATTTATCCACCAGCACTTGTGACTCCTCCTTACCCAAACATGTCACAGGCTGCAATACCACATGGAGCATTACCTCCTCCTCTCCCTTCGACTAATGCTACTTCTGGTTGTGCTGCACAAGGACCAGCATCGTATAAAGCTTTGTCTCCAGCACAACCACAGGCCGGTCTTCAGCATGAGGGCTCCCATAATTTTGAAGGTGGAAGTGAGTGTGAAGCAGGTTCTCTAGCTAGAGATGGAGTATCATCAAATGGAAGTGTGATGTTGGATTTTTCTTCACCTCCACCTAAGCCATCAGATGACAAAGTTGATCATAAGATTGATTCTTTCTGCCAGCTTACTGCTATGAGTGAAGGGAAAATTGAATCACCATTGAAGTCTTCGTGGATTGACTCTTCATCTCAACCTGACCATTTTATCGTCTCTTCAGGTTGTAGCTTGCCTGCTGATTCTGACATGGAGATGGAAGATGATATCACCTTGTCTGACGGAGATCAGGAAGTTCATGATTCATCTAACGCTCTAAACCACAAGTCTGATGTCAGTCATGGGGAGCTTGATGTCAAAAAGCACCTACATGGAGCACAGAGTTCACTAGGATGGACTGCAGCCCAGGGTGTCTCATGTGAAAATGTGTCGTCCTCTTTGTCCCAATGCCAATTGATGATTGAAAAGGCGCCATCTAGAGTTGATATTTATTCAACTGAAGCTTTTGAATCCCTTTTGGCAAGTGAGCGAGATAAAACTTCTATTCCCATAGCTGATGATCAGAACACACTCGATGCTTCTGCAGCGGCTGAGGCAATAAATTCAGACGGGTTTTCTAACCAGATCACTGCAGTTAACAGTCCATTTGGACTTCTACAAGACTATACTTCTGAAAACAGCTCAGAAAATGATCCTCATGCTTTCTTTGAAGATGCTAATGTTGTTATAGCTTCATCATCAGTTACAGCTAGTGTTAAAAGTAGCCATAAAGATGCTGGGTCTCAATTTGAGACAGGATCTAAGAGCCCGTGCATGACTGATAAAATGTCTAGTCTGCAATATGAATCCAGGAGGTCCAAATTTTCCTTGGATACAAAAAAAGAGGTTAAGGGTACTGGTACAACATTAATCATTGAGAGTCATGAGGCCTTCCAGGGAAAAGAGGCGTTGAGTGGTGCTCCTGTTGATATAGTTTCCAAAAGGGATGAGTCTCAGGAGGGGAAAAAGACCAAGTCTGAATCTGTTCTGCCGAAGGTGGATGAGTATGGGAGATTGGTCAGAGAAGGTTCCACTGACAGTAACTCTGATGGTTCACGTTATAATAAGAGGCGTAAAAGAGGCAGAAGCAGAAGCCGCAGCCGCAGTCGCAGCCGTATCCGGAGCCGCAGCCGCAGCCGCAGCCGCAGTTGTAGCCGGTCTCCTCTTGACAGCAGGAGGAGGAGGAGGAGTCCTCGGAGGAGAAGAGAGAAGCGAAACCGTTCTCCCAGTTGGTCTTCTAGGAATCATAGAAGCAGGAGCAGGTCTCCCACTTTTAGGCGTGCAGGTGATTTCAGAGATGAAAATAAGAGACAGGACAGGCGCCACATTCCAGAATGCTTTGACTTCCATAGAGGCAGATGCTTCCGTGGAGCATCCTGTCGGTATATGCACCGTGCACATGACCAAAGTGATGGTTCATGGCGCCATAGGAGCAACCAAAAGCATCTAGAAGTTCAGCCTAGTGTGAAGAACTCTAGGATTAAGGAAGAGATTGAAGATAGCTCTGACATGAATCCGCATGGTGAAATCAAAGGTCAAGAGATGCAACAATGTCAAGATGTGGCCACAAAAGATGGTCAATTTATTGATCCTGATAAAAATGATTGTGAGAGCTCTAGAGTGACAGCTGCTACAATGCAACTGAAACAAAATTTTCAAGTAAAGTCGGAAGGAACCATCGGTCACAATCCTGAATCTCACCAGCCATCCCCTCAGATGTTGAGCTGTGCTGATAATATGAAGTCGTGGGGTGATACTTCTCAGGCTATTGAACAATCTCGAAGCAATAATTCTGCAGGACAGCTTCAGAAGGCTCATTTTTCGTCTCAGCAGATGGAAGCCTCTCTCATATCTGATTCACCACCAGATCGAGCATCCAAGATTTCTCCAAATAAGGTTTCTAGTATTGAACATGCAACAGATGCACTCTTATCAACTCATTCACGTCCCACTGAATGTTCTAATGCTCAACCACTTTCATCAGGACAGTTTTTATCACAGTATATGGCTCCTAAAGAGTCACCTCTTCCCAGTTTTTCTGCTGCAAATAGTCCATATCCGTCACAGCTGCCTCCTCCTCCTCCTCCTCCTTCACTATCACAAGGTACCAGTGTTGCTCATGTGCCACAGGTGCATAGGGACTACAACCAGAGGCCACCCTATCCAGTTCAGTCCATTCCTACAGGAATGATTCATTCTTATCAAGGTCCCTTACCCAACCAACCTGCCCAATTCTCGGTGTCACAAGATTCACCTTGGACATCCTTGCCGCCGCCACCTCCACGACCACCGTATGATTCATCTTTAAATGCAGGAACTGCTGCACGAGGTGCTTCTTCACAATTTCAGCAGAATAACTTGGCTCCAAGGAGTGACTTCAGTTCTCAGAGTTCTATGAGGCCTTACGGCTCTTACCCAACTGAATCCCCTCATTCTAAGGGTGACTTTCTGCATCAGATGTACCCTACATTGTCAGAGCTTCCTCATCCTCTACCGAACAGGGAAGATTTCGGATCAGGCAATCCATCAAATCAACCTTTTGGGGGCCCTGGTCATATGAGAGAAGATCGCTTTACACACGCTCCTGTGCAGGATGTAAATCCTTCACATTCTTTTGCTCAGGGACACCTACACCCTCAACCTGCACCTTCTTCACAGGAGTTAACAAGGATCAAAATGCAAAATTTCTCTGGTGACAATTTTCCATCTGGAGAACTTTTAAATTCATCATCACAAATTCATCCTCATTCACATAACCAACAGCCAAGCTATGGTGTGCAATACTCTGTTGGTGATAGTATTTTGGGTGTGCCTGGGAAGACTGGTGTACAACACCCTGTAGGTGGAAGCATTTTGGGTTTGCCTGGGAAAGATGGCCCCATGTCACGATACCCTCCAGATATGCTGGACAGGAGTCAGTTTTCTCAAGTTCCTGATTTTGGGGCATCTAGAATTCAAACACATCATAATCCTTACGCAGCTACTTTTGAGCAGCCTCTAAGCTCCAAATTCAGTTCTAACATTCTCATTCAAGAAAAGAATGCACCCGGTGGTAACATGTTTGATACACCGGTCCAAGTCCCAGTTGATGGGCAAGGTGTTGGCAGTGCTGGGTCAAGACAGACAACTTCCTCGCCGAGTTCTGCTAGAGCTGGTGGTCAGCTTTTGCCCAAGTCAGATTGTGAGCAGTATGATCCACTTTTTGACAGCATTGAGCCATCAGGAAATCCACTCAATAAACATGATCACAGCCAAAAGCATATACCCACCTGTGATTCGAATATGATGGTGAGGCTCAGTGGTTCTTGTGAGCCATTAGATGTAGAAGAGAACAACAAACATACAGAGGTTCGCCCTGTTTCTTCGGCTACATCTCTGGATAATGACGGCTATGGTGAGACAGCAGATGCAGAAGTGGGTGTTGTTGAGGATGAGAGTCTTAGTAATGATGATGGTGCAGCAAACATGGCTGTAGGAGAGATGGAGATTGATCAGGTGAAATCTGGAGGGAAGAGCAAGAAGAAAAAGGATTCCAGATCAACGAGACTTTTCAAGAGTGCTATTGCAGATTTTGTAAAGGATCTTCTGAAGCCGTCATGGCGGCAGGGTAATATGAGCAAAGAAGCTTTCAAGACCATTGTCAAAAAGACTGTTGATAAGGTTTCTGGAGCCATGAAAAGCCACCAGATACCCAAGTCCGAAGCAAAGATAAATCACTACATTGACTCGTCTCAGCGGAAACTGACAAAGCTTGTGATGGGTTATGTTGATAAGTATGTCAAGGTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001101 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004683 GO:0005488 GO:0005515 GO:0005516 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0009719 GO:0009725 GO:0009737 GO:0009738 GO:0009755 GO:0009931 GO:0009987 GO:0010033 GO:0010857 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0018105 GO:0018193 GO:0018209 GO:0019538 GO:0023052 GO:0032870 GO:0033993 GO:0035556 GO:0036211 GO:0042221 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0046777 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0065007 GO:0070887 GO:0071215 GO:0071229 GO:0071310 GO:0071396 GO:0071495 GO:0071704 GO:0071944 GO:0097305 GO:0097306 GO:0140096 GO:1901564 GO:1901700 GO:1901701
KEGG Pathways
Metabolic & Signaling
ko04012 ko04020 ko04024 ko04066 ko04114 ko04217 ko04261 ko04310 ko04360 ko04713 ko04720 ko04722 ko04725 ko04728 ko04740 ko04745 ko04750 ko04911 ko04912 ko04916 ko04921 ko04922 ko04925 ko04934 ko04971 ko05031 ko05152 ko05200 ko05205 ko05214 map04012 map04020 map04024 map04066 map04114 map04217 map04261 map04310 map04360 map04713 map04720 map04722 map04725 map04728 map04740 map04745 map04750 map04911 map04912 map04916 map04921 map04922 map04925 map04934 map04971 map05031 map05152 map05200 map05205 map05214
Pfam Domains
Protein Families
Pkinase

Protein Analysis

1447

Amino Acids

157.48

Weight (kDa)

6.94

Isoelectric Point (pI)

69.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SCAF11-like_C PF23030 1364 - 1431 5.6e-06 SCAF11-like, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1617
AarI CACCTGC 2 cut(s) 58, 1829
Acc36I ACCTGC 6 cut(s) 58, 518, 1806, 1829, 2788, 3205
Acc65I GGTACC 1 cut(s) 2660
AccB1I GGYRCC 4 cut(s) 985, 1875, 1977, 2660
AccB7I CCANNNNNTGG 2 cut(s) 3326, 3644
AccIII TCCGGA 1 cut(s) 1674
AclWI GGATC 7 cut(s) 1202, 1321, 2138, 3076, 3233, 3743, 4143
AcoI YGGCCR 3 cut(s) 7, 34, 2118
AcsI RAATTY 9 cut(s) 298, 1117, 1376, 2206, 3238, 3277, 3291, 3507, 3563
AcuI CTGAAG 8 cut(s) 68, 264, 464, 720, 1035, 2345, 2917, 4163
AcyI GRCGYC 3 cut(s) 986, 1876, 1978
AdeI CACNNNGTG 2 cut(s) 2885, 3605
AfaI GTAC 5 cut(s) 1411, 1417, 2662, 3017, 3384
AflIII ACRYGT 2 cut(s) 348, 3612
AgeI ACCGGT 1 cut(s) 3625
AjiI CACGTC 1 cut(s) 2501
AjnI CCWGG 7 cut(s) 36, 921, 1364, 1449, 3103, 3367, 3418
AjuI GAANNNNNNNTTGG 2 cut(s) 2437, 2469
Alw21I GWGCWC 4 cut(s) 895, 1477, 1954, 2171
Alw44I GTGCAC 1 cut(s) 1950
AlwI GGATC 7 cut(s) 1202, 1321, 2138, 3076, 3233, 3743, 4143
AlwNI CAGNNNCTG 2 cut(s) 1106, 2873
Aor13HI TCCGGA 1 cut(s) 1674
AoxI GGCC 9 cut(s) 7, 34, 471, 1443, 2118, 2708, 2954, 3100, 3430
ApaI GGGCCC 1 cut(s) 3104
ApaLI GTGCAC 1 cut(s) 1950
ApoI RAATTY 9 cut(s) 298, 1117, 1376, 2206, 3238, 3277, 3291, 3507, 3563
AseI ATTAAT 1 cut(s) 1424
AsiGI ACCGGT 1 cut(s) 3625
Asp700I GAANNNNTTC 2 cut(s) 1539, 1774
Asp718I GGTACC 1 cut(s) 2660
AspLEI GCGC 4 cut(s) 28, 988, 1878, 1980
AspS9I GGNCC 9 cut(s) 65, 143, 431, 1371, 2762, 3100, 3101, 3431, 3628
AsuC2I CCSGG 2 cut(s) 141, 3603
AsuII TTCGAA 1 cut(s) 3833
AvaII GGWCC 6 cut(s) 65, 143, 431, 1371, 2762, 3628
BaeGI GKGCMC 2 cut(s) 1954, 3104
BaeI ACNNNNGTAYC 6 cut(s) 2652, 2685, 3612, 3645, 4235, 4268
BalI TGGCCA 3 cut(s) 9, 36, 2120
BanI GGYRCC 4 cut(s) 985, 1875, 1977, 2660
BanII GRGCYC 4 cut(s) 494, 1325, 2171, 3104
BarI GAAGNNNNNNTAC 2 cut(s) 4122, 4154
BauI CACGAG 1 cut(s) 2880
BbsI GAAGAC 4 cut(s) 470, 681, 1782, 3380
Bbv12I GWGCWC 4 cut(s) 895, 1477, 1954, 2171
BbvCI CCTCAGC 1 cut(s) 1107
BceAI ACGGC 5 cut(s) 13, 1653, 2977, 3958, 4132
BcgI CGANNNNNNTGC 2 cut(s) 1079, 1113
BciT130I CCWGG 7 cut(s) 38, 923, 1366, 1451, 3105, 3369, 3420
BciVI GTATCC 2 cut(s) 1381, 1682
BclI TGATCA 4 cut(s) 619, 1075, 3799, 4045
BcnI CCSGG 2 cut(s) 141, 3603
BfmI CTRYAG 7 cut(s) 915, 1098, 1146, 2349, 2736, 3393, 4026
BfoI RGCGCY 3 cut(s) 989, 1879, 1981
BfuAI ACCTGC 6 cut(s) 58, 518, 1806, 1829, 2788, 3205
BfuI GTATCC 2 cut(s) 1381, 1682
BglI GCCNNNNNGGC 1 cut(s) 4153
Bme1390I CCNGG 9 cut(s) 38, 141, 923, 1366, 1451, 3105, 3369, 3420, 3603
Bme18I GGWCC 6 cut(s) 65, 143, 431, 1371, 2762, 3628
BmgBI CACGTC 1 cut(s) 2501
BmgT120I GGNCC 9 cut(s) 65, 143, 431, 1371, 2762, 3100, 3101, 3431, 3628
BmrFI CCNGG 9 cut(s) 38, 141, 923, 1366, 1451, 3105, 3369, 3420, 3603
BmrI ACTGGG 3 cut(s) 1776, 2580, 3632
BmuI ACTGGG 3 cut(s) 1776, 2580, 3632
BoxI GACNNNNGTC 2 cut(s) 3468, 4193
BpiI GAAGAC 4 cut(s) 470, 681, 1782, 3380
BplI GAGNNNNNCTC 6 cut(s) 1459, 1491, 1732, 1764, 1804, 1836
BpmI CTGGAG 6 cut(s) 87, 441, 3285, 3435, 4080, 4242
Bpu10I CCTNAGC 3 cut(s) 597, 1107, 3174
Bpu14I TTCGAA 1 cut(s) 3833
BpuEI CTTGAG 1 cut(s) 3465
BpuMI CCSGG 2 cut(s) 141, 3603
BsaBI GATNNNNATC 1 cut(s) 3153
BsaHI GRCGYC 3 cut(s) 986, 1876, 1978
BsaI GGTCTC 3 cut(s) 1302, 1719, 1824
BsaWI WCCGGW 2 cut(s) 1674, 3625
BsaXI ACNNNNNCTCC 4 cut(s) 1729, 1759, 3121, 3151
Bse118I RCCGGY 3 cut(s) 473, 1710, 3625
Bse1I ACTGG 7 cut(s) 1417, 1782, 2586, 2664, 2720, 3382, 3638
Bse8I GATNNNNATC 1 cut(s) 3153
BseAI TCCGGA 1 cut(s) 1674
BseBI CCWGG 7 cut(s) 38, 923, 1366, 1451, 3105, 3369, 3420
BseJI GATNNNNATC 1 cut(s) 3153
BseNI ACTGG 7 cut(s) 1417, 1782, 2586, 2664, 2720, 3382, 3638
BseSI GKGCMC 2 cut(s) 1954, 3104
BseYI CCCAGC 2 cut(s) 1292, 3665
BsgI GTGCAG 6 cut(s) 408, 1857, 2862, 3164, 3183, 4032
BshFI GGCC 9 cut(s) 9, 36, 473, 1445, 2120, 2710, 2956, 3102, 3432
BshNI GGYRCC 4 cut(s) 985, 1875, 1977, 2660
BshTI ACCGGT 1 cut(s) 3625
BsiHKAI GWGCWC 4 cut(s) 895, 1477, 1954, 2171
BsiSI CCGG 6 cut(s) 140, 474, 1675, 1711, 3603, 3626
BslFI GGGAC 6 cut(s) 943, 2487, 2708, 2748, 3192, 3620
BsmBI CGTCTC 3 cut(s) 736, 2385, 4290
BsmFI GGGAC 6 cut(s) 943, 2487, 2708, 2748, 3192, 3620
BsmI GAATGC 2 cut(s) 1895, 3601
BsnI GGCC 9 cut(s) 9, 36, 473, 1445, 2120, 2710, 2956, 3102, 3432
Bso31I GGTCTC 3 cut(s) 1302, 1719, 1824
Bsp119I TTCGAA 1 cut(s) 3833
Bsp120I GGGCCC 1 cut(s) 3100
Bsp1286I GDGCHC 7 cut(s) 494, 895, 1325, 1477, 1954, 2171, 3104
Bsp13I TCCGGA 1 cut(s) 1674
Bsp1407I TGTACA 1 cut(s) 3382
Bsp19I CCATGG 1 cut(s) 247
BspANI GGCC 9 cut(s) 9, 36, 473, 1445, 2120, 2710, 2956, 3102, 3432
BspEI TCCGGA 1 cut(s) 1674
BspHI TCATGA 2 cut(s) 814, 1438
BspMAI CTGCAG 4 cut(s) 919, 1102, 1150, 2353
BspMI ACCTGC 6 cut(s) 58, 518, 1806, 1829, 2788, 3205
BspPI GGATC 7 cut(s) 1202, 1321, 2138, 3076, 3233, 3743, 4143
BspQI GCTCTTC 1 cut(s) 4061
BspT104I TTCGAA 1 cut(s) 3833
BspT107I GGYRCC 4 cut(s) 985, 1875, 1977, 2660
BspTNI GGTCTC 3 cut(s) 1302, 1719, 1824
BsrFI RCCGGY 3 cut(s) 473, 1710, 3625
BsrGI TGTACA 1 cut(s) 3382
BsrI ACTGG 7 cut(s) 1417, 1782, 2586, 2664, 2720, 3382, 3638
BssAI RCCGGY 3 cut(s) 473, 1710, 3625
BssNI GRCGYC 3 cut(s) 986, 1876, 1978
BssSI CACGAG 1 cut(s) 2880
BssT1I CCWWGG 4 cut(s) 247, 1383, 2810, 2921
Bst2BI CACGAG 1 cut(s) 2880
Bst2UI CCWGG 7 cut(s) 38, 923, 1366, 1451, 3105, 3369, 3420
Bst6I CTCTTC 7 cut(s) 709, 739, 1753, 2037, 2586, 3876, 4061
BstACI GRCGYC 3 cut(s) 986, 1876, 1978
BstAPI GCANNNNNTGC 4 cut(s) 268, 2477, 2873, 2885
BstAUI TGTACA 1 cut(s) 3382
BstBI TTCGAA 1 cut(s) 3833
BstC8I GCNNGC 4 cut(s) 644, 749, 753, 1836
BstDSI CCRYGG 2 cut(s) 247, 1921
BstH2I RGCGCY 3 cut(s) 989, 1879, 1981
BstHHI GCGC 4 cut(s) 28, 988, 1878, 1980
BstNI CCWGG 7 cut(s) 38, 923, 1366, 1451, 3105, 3369, 3420
BstNSI RCATGY 4 cut(s) 278, 352, 2471, 3616
BstPAI GACNNNNGTC 2 cut(s) 3468, 4193
BstSCI CCNGG 9 cut(s) 36, 139, 921, 1364, 1449, 3103, 3367, 3418, 3601
BstSFI CTRYAG 7 cut(s) 915, 1098, 1146, 2349, 2736, 3393, 4026
BstSLI GKGCMC 2 cut(s) 1954, 3104
BstV2I GAAGAC 4 cut(s) 470, 681, 1782, 3380
BstX2I RGATCY 2 cut(s) 1313, 4135
BstXI CCANNNNNNTGG 1 cut(s) 1967
BstYI RGATCY 2 cut(s) 1313, 4135
BsuI GTATCC 2 cut(s) 1381, 1682
BsuRI GGCC 9 cut(s) 9, 36, 473, 1445, 2120, 2710, 2956, 3102, 3432
BtgI CCRYGG 2 cut(s) 247, 1921
BtrI CACGTC 1 cut(s) 2501
BtsI GCAGTG 2 cut(s) 1143, 3667
BtsIMutI CAGTG 7 cut(s) 202, 1143, 1587, 2505, 2671, 3667, 3859
BveI ACCTGC 6 cut(s) 58, 518, 1806, 1829, 2788, 3205
Cac8I GCNNGC 4 cut(s) 644, 749, 753, 1836
CaiI CAGNNNCTG 2 cut(s) 1106, 2873
CciI TCATGA 2 cut(s) 814, 1438
CfoI GCGC 4 cut(s) 28, 988, 1878, 1980
Cfr10I RCCGGY 3 cut(s) 473, 1710, 3625
Cfr13I GGNCC 9 cut(s) 65, 143, 431, 1371, 2762, 3100, 3101, 3431, 3628
Csp6I GTAC 5 cut(s) 1410, 1416, 2661, 3016, 3383
CspAI ACCGGT 1 cut(s) 3625
CviQI GTAC 5 cut(s) 1410, 1416, 2661, 3016, 3383
DinI GGCGCC 3 cut(s) 987, 1877, 1979
DraI TTTAAA 2 cut(s) 2862, 3276
DraIII CACNNNGTG 2 cut(s) 2885, 3605
EaeI YGGCCR 3 cut(s) 7, 34, 2118
Eam1104I CTCTTC 7 cut(s) 709, 739, 1753, 2037, 2586, 3876, 4061
EarI CTCTTC 7 cut(s) 709, 739, 1753, 2037, 2586, 3876, 4061
EciI GGCGGA 1 cut(s) 57
Ecl136II GAGCTC 1 cut(s) 2169
Eco130I CCWWGG 4 cut(s) 247, 1383, 2810, 2921
Eco147I AGGCCT 2 cut(s) 1445, 2956
Eco24I GRGCYC 4 cut(s) 494, 1325, 2171, 3104
Eco31I GGTCTC 3 cut(s) 1302, 1719, 1824
Eco32I GATATC 1 cut(s) 784
Eco47I GGWCC 6 cut(s) 65, 143, 431, 1371, 2762, 3628
Eco53kI GAGCTC 1 cut(s) 2169
Eco57I CTGAAG 8 cut(s) 68, 264, 464, 720, 1035, 2345, 2917, 4163
EcoICRI GAGCTC 1 cut(s) 2169
EcoO109I RGGNCCY 4 cut(s) 143, 2762, 3100, 3101
EcoRI GAATTC 1 cut(s) 3507
EcoRII CCWGG 7 cut(s) 36, 921, 1364, 1449, 3103, 3367, 3418
EcoRV GATATC 1 cut(s) 784
EcoT14I CCWWGG 4 cut(s) 247, 1383, 2810, 2921
EcoT38I GRGCYC 4 cut(s) 494, 1325, 2171, 3104
EgeI GGCGCC 3 cut(s) 987, 1877, 1979
EheI GGCGCC 3 cut(s) 987, 1877, 1979
ErhI CCWWGG 4 cut(s) 247, 1383, 2810, 2921
Esp3I CGTCTC 3 cut(s) 736, 2385, 4290
FalI AAGNNNNNCTT 2 cut(s) 2984, 3016
FaqI GGGAC 6 cut(s) 943, 2487, 2708, 2748, 3192, 3620
FauI CCCGC 1 cut(s) 190
FauNDI CATATG 1 cut(s) 3111
FbaI TGATCA 4 cut(s) 619, 1075, 3799, 4045
FriOI GRGCYC 4 cut(s) 494, 1325, 2171, 3104
GlaI GCGC 4 cut(s) 27, 987, 1877, 1979
GsaI CCCAGC 2 cut(s) 1296, 3669
GsuI CTGGAG 6 cut(s) 87, 441, 3285, 3435, 4080, 4242
HaeII RGCGCY 3 cut(s) 989, 1879, 1981
HaeIII GGCC 9 cut(s) 9, 36, 473, 1445, 2120, 2710, 2956, 3102, 3432
HapII CCGG 6 cut(s) 140, 474, 1675, 1711, 3603, 3626
HhaI GCGC 4 cut(s) 28, 988, 1878, 1980
Hin1I GRCGYC 3 cut(s) 986, 1876, 1978
Hin6I GCGC 4 cut(s) 26, 986, 1876, 1978
HinP1I GCGC 4 cut(s) 26, 986, 1876, 1978
HincII GTYRAC 2 cut(s) 1153, 3219
HindII GTYRAC 2 cut(s) 1153, 3219
HindIII AAGCTT 4 cut(s) 447, 1017, 4179, 4304
HpaI GTTAAC 2 cut(s) 1153, 3219
HpaII CCGG 6 cut(s) 140, 474, 1675, 1711, 3603, 3626
Hpy166II GTNNAC 6 cut(s) 902, 1153, 1610, 1952, 3219, 3383
Hpy8I GTNNAC 6 cut(s) 902, 1153, 1610, 1952, 3219, 3383
HpyCH4IV ACGT 3 cut(s) 267, 1612, 2500
HpySE526I ACGT 3 cut(s) 267, 1612, 2500
Hsp92I GRCGYC 3 cut(s) 986, 1876, 1978
HspAI GCGC 4 cut(s) 26, 986, 1876, 1978
KasI GGCGCC 3 cut(s) 985, 1875, 1977
Kpn2I TCCGGA 1 cut(s) 1674
KpnI GGTACC 1 cut(s) 2664
Ksp22I TGATCA 4 cut(s) 619, 1075, 3799, 4045
KspAI GTTAAC 2 cut(s) 1153, 3219
LguI GCTCTTC 1 cut(s) 4061
MaeII ACGT 3 cut(s) 267, 1612, 2500
MfeI CAATTG 1 cut(s) 968
MflI RGATCY 2 cut(s) 1313, 4135
MhlI GDGCHC 7 cut(s) 494, 895, 1325, 1477, 1954, 2171, 3104
MlsI TGGCCA 3 cut(s) 9, 36, 2120
MluNI TGGCCA 3 cut(s) 9, 36, 2120
Mly113I GGCGCC 3 cut(s) 986, 1876, 1978
MlyI GAGTC 9 cut(s) 325, 695, 1444, 1514, 1753, 2582, 3478, 4000, 4274
MmeI TCCRAC 5 cut(s) 115, 263, 328, 555, 2202
Mox20I TGGCCA 3 cut(s) 9, 36, 2120
MroI TCCGGA 1 cut(s) 1674
MroXI GAANNNNTTC 2 cut(s) 1539, 1774
MscI TGGCCA 3 cut(s) 9, 36, 2120
MseI TTAA 8 cut(s) 1152, 1272, 1404, 1424, 2037, 2861, 3218, 3275
MslI CAYNNNNRTG 4 cut(s) 770, 885, 907, 1287
Msp20I TGGCCA 3 cut(s) 9, 36, 2120
MspA1I CMGCKG 5 cut(s) 185, 1103, 2183, 2623, 4291
MspI CCGG 6 cut(s) 140, 474, 1675, 1711, 3603, 3626
MspR9I CCNGG 9 cut(s) 38, 141, 923, 1366, 1451, 3105, 3369, 3420, 3603
MunI CAATTG 1 cut(s) 968
Mva1269I GAATGC 2 cut(s) 1895, 3601
MvaI CCWGG 7 cut(s) 38, 923, 1366, 1451, 3105, 3369, 3420
NarI GGCGCC 3 cut(s) 986, 1876, 1978
NciI CCSGG 2 cut(s) 141, 3603
NcoI CCATGG 1 cut(s) 247
NdeI CATATG 1 cut(s) 3111
NmeAIII GCCGAG 1 cut(s) 3717
NspI RCATGY 4 cut(s) 278, 352, 2471, 3616
NspV TTCGAA 1 cut(s) 3833
PagI TCATGA 2 cut(s) 814, 1438
PaqCI CACCTGC 2 cut(s) 58, 1829
PasI CCCWGGG 1 cut(s) 922
PceI AGGCCT 2 cut(s) 1445, 2956
PciI ACATGT 2 cut(s) 348, 3612
PciSI GCTCTTC 1 cut(s) 4061
PctI GAATGC 2 cut(s) 1895, 3601
PdmI GAANNNNTTC 2 cut(s) 1539, 1774
PflFI GACNNNGTC 1 cut(s) 1528
PflMI CCANNNNNTGG 2 cut(s) 3326, 3644
PfoI TCCNGGA 1 cut(s) 1364
PinAI ACCGGT 1 cut(s) 3625
PleI GAGTC 9 cut(s) 325, 695, 1443, 1513, 1752, 2581, 3477, 3999, 4274
PluTI GGCGCC 3 cut(s) 989, 1879, 1981
PpsI GAGTC 9 cut(s) 325, 695, 1443, 1513, 1752, 2581, 3477, 3999, 4274
PpuMI RGGWCCY 2 cut(s) 143, 2762
PscI ACATGT 2 cut(s) 348, 3612
PshAI GACNNNNGTC 2 cut(s) 3468, 4193
PshBI ATTAAT 1 cut(s) 1424
PsiI TTATAA 1 cut(s) 1617
Psp124BI GAGCTC 1 cut(s) 2171
Psp5II RGGWCCY 2 cut(s) 143, 2762
Psp6I CCWGG 7 cut(s) 36, 921, 1364, 1449, 3103, 3367, 3418
PspFI CCCAGC 2 cut(s) 1292, 3665
PspGI CCWGG 7 cut(s) 36, 921, 1364, 1449, 3103, 3367, 3418
PspOMI GGGCCC 1 cut(s) 3100
PspPI GGNCC 9 cut(s) 65, 143, 431, 1371, 2762, 3100, 3101, 3431, 3628
PspPPI RGGWCCY 2 cut(s) 143, 2762
PstI CTGCAG 4 cut(s) 919, 1102, 1150, 2353
PstNI CAGNNNCTG 2 cut(s) 1106, 2873
PsuI RGATCY 2 cut(s) 1313, 4135
PsyI GACNNNGTC 1 cut(s) 1528
PvuII CAGCTG 2 cut(s) 2183, 2623
RsaI GTAC 5 cut(s) 1411, 1417, 2662, 3017, 3384
RsaNI GTAC 5 cut(s) 1410, 1416, 2661, 3016, 3383
RseI CAYNNNNRTG 4 cut(s) 770, 885, 907, 1287
SacI GAGCTC 1 cut(s) 2171
SapI GCTCTTC 1 cut(s) 4061
SaqAI TTAA 8 cut(s) 1152, 1272, 1404, 1424, 2037, 2861, 3218, 3275
Sau96I GGNCC 9 cut(s) 65, 143, 431, 1371, 2762, 3100, 3101, 3431, 3628
SchI GAGTC 9 cut(s) 325, 695, 1444, 1514, 1753, 2582, 3478, 4000, 4274
ScrFI CCNGG 9 cut(s) 38, 141, 923, 1366, 1451, 3105, 3369, 3420, 3603
SduI GDGCHC 7 cut(s) 494, 895, 1325, 1477, 1954, 2171, 3104
SfcI CTRYAG 7 cut(s) 915, 1098, 1146, 2349, 2736, 3393, 4026
SfoI GGCGCC 3 cut(s) 987, 1877, 1979
SfuI TTCGAA 1 cut(s) 3833
SinI GGWCC 6 cut(s) 65, 143, 431, 1371, 2762, 3628
SmiMI CAYNNNNRTG 4 cut(s) 770, 885, 907, 1287
SmlI CTYRAG 1 cut(s) 3480
SmoI CTYRAG 1 cut(s) 3480
SseBI AGGCCT 2 cut(s) 1445, 2956
SspDI GGCGCC 3 cut(s) 985, 1875, 1977
SstI GAGCTC 1 cut(s) 2171
StuI AGGCCT 2 cut(s) 1445, 2956
StyD4I CCNGG 9 cut(s) 36, 139, 921, 1364, 1449, 3103, 3367, 3418, 3601
StyI CCWWGG 4 cut(s) 247, 1383, 2810, 2921
TaiI ACGT 3 cut(s) 270, 1615, 2503
TaqI TCGA 5 cut(s) 398, 1089, 2335, 2425, 3833
TaqII GACCGA 1 cut(s) 2225
TatI WGTACW 1 cut(s) 3382
Tru1I TTAA 8 cut(s) 1152, 1272, 1404, 1424, 2037, 2861, 3218, 3275
Tru9I TTAA 8 cut(s) 1152, 1272, 1404, 1424, 2037, 2861, 3218, 3275
TscAI CASTG 7 cut(s) 202, 1150, 1594, 2512, 2671, 3667, 3859
TspGWI ACGGA 4 cut(s) 180, 813, 1910, 2604
TspRI CASTG 7 cut(s) 202, 1150, 1594, 2512, 2671, 3667, 3859
Tth111I GACNNNGTC 1 cut(s) 1528
Van91I CCANNNNNTGG 2 cut(s) 3326, 3644
VneI GTGCAC 1 cut(s) 1950
VpaK11BI GGWCC 6 cut(s) 65, 143, 431, 1371, 2762, 3628
VspI ATTAAT 1 cut(s) 1424
XapI RAATTY 9 cut(s) 298, 1117, 1376, 2206, 3238, 3277, 3291, 3507, 3563
XbaI TCTAGA 4 cut(s) 992, 2002, 2171, 3503
XceI RCATGY 4 cut(s) 278, 352, 2471, 3616
XcmI CCANNNNNNNNNTGG 3 cut(s) 17, 1289, 1381
XmnI GAANNNNTTC 2 cut(s) 1539, 1774
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.