Rmu_sc0001308.1_g000003

Calcium-dependent protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0001308.1
OG0002852
Physical Location & Seq
Reverse (-)
10055 .. 14128
4074 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0001308.1_g000003.1.cds

Sequence Viewer

Length: 2355 bp
atggctgttgccaagagcagcagcaacagggaaccatcaacacggctatgcaattcttacaaagtctcgagcttaacgtcctccatcttggatgcaaatgagacctcaaatctaaaagaccagtatattcttggagagcaattaggttggggtcaatttggtgttattagggtgtgcactgataagtttacgggagaggttttggcatgcaaatcaatagctaaagatcgattagtgacctcagatgatgtcagtagcatcaagcttgagattgaaatcatgaccaggttgtctgggcacccaaatgttgtagatctcaaggcggtgtatgaagaggaagagtacgttcatttggtgatggagctctgtgcaggaggggagcttttccaccagttagagaagcacgggcatttctctgaatctgatgcaagggttctctttaggcatctgattcaggtagttttatactgtcatgaaaatggggttgtccacagagatttgaagcctgagaatattctgttggccacaaaagcctcctcatctcctataaaattggcagactttggtcttgcaacttacatcaagcctggccagagtttgcacggtttggtgggaagtccgttttatatagcgcctgaggtacttgccgggggctataaccaagctgctgatgtttggagtgcaggagtaattctttacattcttctcagcggaatgccaccattttgggggaagacaaagtcaagcatatttgatgctgttaggacagctgatctgaggttcccatctgatccctgggatggcattactgaatcagctaatgatttgattcgaggaatgctctgtaaagatccttctaaaaggctcaccgctcaccaggttttagagcattcctggatgaggagtactgaatccctttctaaacaacctagtcaatgtaaaaatctaggttataaagaatgtgatgtgggcagtagctcatttaccacctcatttatgtccaggagtcaggacatcagctttggtaccgcatcacctagtatatgtgaagcccaatcccctacattcacaagcaggtcatctttttctcctttcctggtagagccatctactccttgcttggcatctggtgggttgtcattccgtagcaatgacgtctccaattgcctggaattctcctcccctatttcctcaatgccaagctttgcattcttcagccccagttctgtggttgaggaacgtagttgttcggtggagttctcagccagcatagatgcagttcatcaagagacaagctcagggaagttgcttctattatcagattccctttcatttaggcttgaagctagaggaatgattaacagaccagcagtagctaaaagatccggggtgacaactgggtctaggacattgggcatccacagcaagaggaaccgcacaattggactcggtgagcacgagcaactagatttcatggtcaacgaatcaatcatccgctgtgccacttttgatgttagttgcaagtatgatgatgatttggcttttaccagtaggaaaactgaaacagaagggaattgttacacagtcaattctcacagcttcttcagaaaacccaaaaccttccactctaccttcacaagacccagaaggaggtactggcaaatgggttgcaagataaacaactcaaaccctccaattctttggaagatcccaactaagcagcagaggcctagaacaaaaaccatctccttctcttccaccaccacagactctcaaacccaatatcaaaactctaagctttacactgtcaagttcaagaccttgagcgcctgcaagcttggcatagccagatattccgattttgaatacaatgctgaaggtggcggaggaaccgggtctggtacaacagaaagcagcaactcgagtggtgaagtttcagtgtcatttgacatcgcaacactctacatcccaccattgcagacttcaacaacaaagtttcttggattgccattgccaccatttttgaagattgacattgtcccggagctttttcgtggcagcatcaatcaagaatccggcaaggttgatcttgagttcaaggccaagttctggttttcggttggaagcatctatagagctcctccgcttgtagtggagacggttttgacatctgaggaatcgaaaggaacaatgagaagtggaaaaggggagaggttggatgaactaggaaactgcaaactggttggagtggcaactgttgatcccattgatgactttttcattaacaccttccttggcctccctactgaatgccttgcaggcatgaatgccattatttctttgtctacttgttag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001101 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004683 GO:0005488 GO:0005515 GO:0005516 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0009719 GO:0009725 GO:0009737 GO:0009738 GO:0009755 GO:0009931 GO:0009987 GO:0010033 GO:0010857 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0018105 GO:0018193 GO:0018209 GO:0019538 GO:0023052 GO:0032870 GO:0033993 GO:0035556 GO:0036211 GO:0042221 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0046777 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0065007 GO:0070887 GO:0071215 GO:0071229 GO:0071310 GO:0071396 GO:0071495 GO:0071704 GO:0071944 GO:0097305 GO:0097306 GO:0140096 GO:1901564 GO:1901700 GO:1901701
KEGG Pathways
Metabolic & Signaling
ko04012 ko04020 ko04024 ko04066 ko04114 ko04217 ko04261 ko04310 ko04360 ko04713 ko04720 ko04722 ko04725 ko04728 ko04740 ko04745 ko04750 ko04911 ko04912 ko04916 ko04921 ko04922 ko04925 ko04934 ko04971 ko05031 ko05152 ko05200 ko05205 ko05214 map04012 map04020 map04024 map04066 map04114 map04217 map04261 map04310 map04360 map04713 map04720 map04722 map04725 map04728 map04740 map04745 map04750 map04911 map04912 map04916 map04921 map04922 map04925 map04934 map04971 map05031 map05152 map05200 map05205 map05214
Pfam Domains
Protein Families
Pkinase

Protein Analysis

784

Amino Acids

86.19

Weight (kDa)

6.77

Isoelectric Point (pI)

47.27

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 954
AasI GACNNNNNNGTC 2 cut(s) 289, 1399
AatII GACGTC 1 cut(s) 1158
Acc36I ACCTGC 1 cut(s) 1065
Acc65I GGTACC 1 cut(s) 1025
AccB1I GGYRCC 2 cut(s) 297, 1025
AccB7I CCANNNNNTGG 1 cut(s) 2109
AccBSI CCGCTC 1 cut(s) 872
AccI GTMKAC 1 cut(s) 2345
AciI CCGC 8 cut(s) 323, 711, 870, 1029, 1435, 1495, 1884, 2144
AclWI GGATC 5 cut(s) 785, 845, 1377, 1702, 2255
AcoI YGGCCR 2 cut(s) 522, 589
AcsI RAATTY 1 cut(s) 1172
AcuI CTGAAG 3 cut(s) 1198, 1588, 1896
AcyI GRCGYC 1 cut(s) 1155
AfaI GTAC 6 cut(s) 344, 642, 907, 1027, 1655, 1903
AfiI CCNNNNNNNGG 5 cut(s) 728, 800, 900, 1650, 2109
AgsI TTSAA 8 cut(s) 275, 502, 1343, 1816, 1865, 1986, 2026, 2098
AjnI CCWGG 8 cut(s) 284, 586, 794, 876, 893, 1001, 1095, 1167
AjuI GAANNNNNNNTTGG 2 cut(s) 503, 535
Alw21I GWGCWC 4 cut(s) 179, 366, 1458, 2140
Alw26I GTCTC 5 cut(s) 70, 95, 1162, 1283, 2150
Alw44I GTGCAC 1 cut(s) 175
AlwI GGATC 5 cut(s) 785, 845, 1377, 1702, 2255
Ama87I CYCGRG 2 cut(s) 67, 1921
AoxI GGCC 5 cut(s) 522, 589, 1727, 2100, 2296
ApaLI GTGCAC 1 cut(s) 175
ApeKI GCWGC 6 cut(s) 18, 21, 665, 1720, 1914, 2058
ApoI RAATTY 1 cut(s) 1172
ArsI GACNNNNNNTTYG 2 cut(s) 1004, 1036
Asp718I GGTACC 1 cut(s) 1025
AspLEI GCGC 2 cut(s) 634, 1829
AsuC2I CCSGG 4 cut(s) 649, 1387, 1894, 2042
AsuHPI GGTGA 7 cut(s) 367, 859, 866, 1026, 1402, 1463, 1940
AvaI CYCGRG 2 cut(s) 67, 1921
AxyI CCTNAGG 1 cut(s) 636
BaeGI GKGCMC 2 cut(s) 179, 300
BalI TGGCCA 2 cut(s) 524, 591
BanI GGYRCC 2 cut(s) 297, 1025
BanII GRGCYC 2 cut(s) 366, 2140
BarI GAAGNNNNNNTAC 2 cut(s) 838, 870
BauI CACGAG 1 cut(s) 1457
BbsI GAAGAC 1 cut(s) 740
Bbv12I GWGCWC 4 cut(s) 179, 366, 1458, 2140
BbvI GCAGC 6 cut(s) 30, 33, 652, 1732, 1926, 2070
BccI CCATC 7 cut(s) 43, 92, 352, 793, 794, 1114, 1751
BceAI ACGGC 1 cut(s) 59
BciT130I CCWGG 8 cut(s) 286, 588, 796, 878, 895, 1003, 1097, 1169
BcnI CCSGG 4 cut(s) 649, 1387, 1894, 2042
BcoDI GTCTC 5 cut(s) 70, 95, 1162, 1283, 2150
BfaI CTAG 8 cut(s) 930, 947, 1038, 1347, 1404, 1466, 1731, 2225
BfmI CTRYAG 1 cut(s) 2131
BfoI RGCGCY 2 cut(s) 635, 1830
BfuAI ACCTGC 1 cut(s) 1065
BglI GCCNNNNNGGC 1 cut(s) 2319
BglII AGATCT 1 cut(s) 313
BisI GCNGC 6 cut(s) 19, 22, 666, 1721, 1915, 2059
BlsI GCNGC 6 cut(s) 20, 23, 667, 1722, 1916, 2060
BmcAI AGTACT 1 cut(s) 907
BmeT110I CYCGRG 2 cut(s) 67, 1921
BmiI GGNNCC 6 cut(s) 33, 299, 782, 1027, 1433, 1891
BmrI ACTGGG 2 cut(s) 1215, 1407
BmuI ACTGGG 2 cut(s) 1215, 1407
BoxI GACNNNNGTC 1 cut(s) 564
BpiI GAAGAC 1 cut(s) 740
BplI GAGNNNNNCTC 4 cut(s) 825, 857, 1280, 1312
Bpu10I CCTNAGC 1 cut(s) 1297
BpuEI CTTGAG 4 cut(s) 287, 302, 1843, 2111
BpuMI CCSGG 4 cut(s) 649, 1387, 1894, 2042
Bsa29I ATCGAT 1 cut(s) 229
BsaBI GATNNNNATC 1 cut(s) 275
BsaHI GRCGYC 1 cut(s) 1155
BsaI GGTCTC 1 cut(s) 95
BsaJI CCNNGG 5 cut(s) 648, 794, 795, 1386, 2293
Bsc4I CCNNNNNNNGG 5 cut(s) 728, 800, 900, 1650, 2109
Bse1I ACTGG 7 cut(s) 121, 391, 1221, 1402, 1548, 1661, 2244
Bse21I CCTNAGG 1 cut(s) 636
Bse3DI GCAATG 3 cut(s) 1156, 1973, 2009
Bse8I GATNNNNATC 1 cut(s) 275
BseBI CCWGG 8 cut(s) 286, 588, 796, 878, 895, 1003, 1097, 1169
BseCI ATCGAT 1 cut(s) 229
BseDI CCNNGG 5 cut(s) 648, 794, 795, 1386, 2293
BseGI GGATG 7 cut(s) 97, 805, 903, 1416, 1491, 1965, 2224
BseJI GATNNNNATC 1 cut(s) 275
BseLI CCNNNNNNNGG 5 cut(s) 728, 800, 900, 1650, 2109
BseMI GCAATG 3 cut(s) 1156, 1973, 2009
BseMII CTCAG 8 cut(s) 255, 498, 627, 721, 767, 1275, 1311, 2163
BseNI ACTGG 7 cut(s) 121, 391, 1221, 1402, 1548, 1661, 2244
BseRI GAGGAG 4 cut(s) 526, 916, 1168, 2130
BseSI GKGCMC 2 cut(s) 179, 300
BseXI GCAGC 6 cut(s) 30, 33, 652, 1732, 1926, 2070
BsgI GTGCAG 2 cut(s) 390, 702
BshFI GGCC 5 cut(s) 524, 591, 1729, 2102, 2298
BshNI GGYRCC 2 cut(s) 297, 1025
BshVI ATCGAT 1 cut(s) 229
BsiHKAI GWGCWC 4 cut(s) 179, 366, 1458, 2140
BsiHKCI CYCGRG 2 cut(s) 67, 1921
BsiSI CCGG 5 cut(s) 648, 1386, 1893, 2042, 2076
BslFI GGGAC 1 cut(s) 2024
BslI CCNNNNNNNGG 5 cut(s) 728, 800, 900, 1650, 2109
BsmAI GTCTC 5 cut(s) 70, 95, 1162, 1283, 2150
BsmBI CGTCTC 2 cut(s) 1162, 2150
BsmFI GGGAC 1 cut(s) 2024
BsmI GAATGC 6 cut(s) 720, 843, 889, 1208, 2315, 2332
BsnI GGCC 5 cut(s) 524, 591, 1729, 2102, 2298
Bso31I GGTCTC 1 cut(s) 95
BsoBI CYCGRG 2 cut(s) 67, 1921
Bsp1286I GDGCHC 5 cut(s) 179, 300, 366, 1458, 2140
Bsp143I GATC 9 cut(s) 226, 313, 772, 790, 850, 1382, 1707, 2086, 2260
BspACI CCGC 8 cut(s) 323, 711, 870, 1029, 1435, 1495, 1884, 2144
BspANI GGCC 5 cut(s) 524, 591, 1729, 2102, 2298
BspCNI CTCAG 8 cut(s) 254, 499, 628, 720, 768, 1274, 1310, 2164
BspDI ATCGAT 1 cut(s) 229
BspHI TCATGA 2 cut(s) 279, 472
BspLI GGNNCC 6 cut(s) 33, 299, 782, 1027, 1433, 1891
BspMI ACCTGC 1 cut(s) 1065
BspPI GGATC 5 cut(s) 785, 845, 1377, 1702, 2255
BspT107I GGYRCC 2 cut(s) 297, 1025
BspTNI GGTCTC 1 cut(s) 95
BsrBI CCGCTC 1 cut(s) 872
BsrDI GCAATG 3 cut(s) 1156, 1973, 2009
BsrI ACTGG 7 cut(s) 121, 391, 1221, 1402, 1548, 1661, 2244
BssECI CCNNGG 5 cut(s) 648, 794, 795, 1386, 2293
BssMI GATC 9 cut(s) 226, 313, 772, 790, 850, 1382, 1707, 2086, 2260
BssNI GRCGYC 1 cut(s) 1155
BssSI CACGAG 1 cut(s) 1457
BssT1I CCWWGG 1 cut(s) 2293
Bst2BI CACGAG 1 cut(s) 1457
Bst2UI CCWGG 8 cut(s) 286, 588, 796, 878, 895, 1003, 1097, 1169
Bst4CI ACNGT 6 cut(s) 470, 605, 1585, 1807, 2161, 2257
Bst6I CTCTTC 3 cut(s) 327, 333, 1759
BstACI GRCGYC 1 cut(s) 1155
BstC8I GCNNGC 5 cut(s) 208, 1267, 1831, 1835, 2320
BstENI CCTNNNNNAGG 1 cut(s) 898
BstF5I GGATG 7 cut(s) 97, 805, 903, 1416, 1491, 1965, 2224
BstH2I RGCGCY 2 cut(s) 635, 1830
BstHHI GCGC 2 cut(s) 634, 1829
BstKTI GATC 9 cut(s) 229, 316, 775, 793, 853, 1385, 1710, 2089, 2263
BstMAI GTCTC 5 cut(s) 70, 95, 1162, 1283, 2150
BstMBI GATC 9 cut(s) 226, 313, 772, 790, 850, 1382, 1707, 2086, 2260
BstMWI GCNNNNNNNGC 5 cut(s) 530, 1422, 1726, 1839, 2319
BstNI CCWGG 8 cut(s) 286, 588, 796, 878, 895, 1003, 1097, 1169
BstNSI RCATGY 1 cut(s) 210
BstPAI GACNNNNGTC 1 cut(s) 564
BstSFI CTRYAG 1 cut(s) 2131
BstSLI GKGCMC 2 cut(s) 179, 300
BstV1I GCAGC 6 cut(s) 30, 33, 652, 1732, 1926, 2070
BstV2I GAAGAC 1 cut(s) 740
BstX2I RGATCY 4 cut(s) 313, 850, 1382, 1707
BstXI CCANNNNNNTGG 4 cut(s) 726, 1168, 1228, 1701
BstYI RGATCY 4 cut(s) 313, 850, 1382, 1707
Bsu15I ATCGAT 1 cut(s) 229
Bsu36I CCTNAGG 1 cut(s) 636
BsuRI GGCC 5 cut(s) 524, 591, 1729, 2102, 2298
BsuTUI ATCGAT 1 cut(s) 229
BtgZI GCGATG 1 cut(s) 1936
BtsCI GGATG 7 cut(s) 97, 805, 903, 1416, 1491, 1965, 2224
BtsIMutI CAGTG 3 cut(s) 177, 1803, 1944
BveI ACCTGC 1 cut(s) 1065
Cac8I GCNNGC 5 cut(s) 208, 1267, 1831, 1835, 2320
CciI TCATGA 2 cut(s) 279, 472
CfoI GCGC 2 cut(s) 634, 1829
ClaI ATCGAT 1 cut(s) 229
CsiI ACCWGGT 2 cut(s) 284, 876
Csp6I GTAC 6 cut(s) 343, 641, 906, 1026, 1654, 1902
CspCI CAANNNNNGTGG 4 cut(s) 1906, 1941, 2004, 2039
CviAII CATG 5 cut(s) 207, 280, 473, 1474, 2323
CviQI GTAC 6 cut(s) 343, 641, 906, 1026, 1654, 1902
DpnI GATC 9 cut(s) 228, 315, 774, 792, 852, 1384, 1709, 2088, 2262
DpnII GATC 9 cut(s) 226, 313, 772, 790, 850, 1382, 1707, 2086, 2260
DrdI GACNNNNNNGTC 2 cut(s) 289, 1399
DseDI GACNNNNNNGTC 2 cut(s) 289, 1399
EaeI YGGCCR 2 cut(s) 522, 589
Eam1104I CTCTTC 3 cut(s) 327, 333, 1759
EarI CTCTTC 3 cut(s) 327, 333, 1759
EciI GGCGGA 1 cut(s) 1899
Ecl136II GAGCTC 2 cut(s) 364, 2138
Eco130I CCWWGG 1 cut(s) 2293
Eco147I AGGCCT 1 cut(s) 1729
Eco24I GRGCYC 2 cut(s) 366, 2140
Eco31I GGTCTC 1 cut(s) 95
Eco53kI GAGCTC 2 cut(s) 364, 2138
Eco57I CTGAAG 3 cut(s) 1198, 1588, 1896
Eco81I CCTNAGG 1 cut(s) 636
Eco88I CYCGRG 2 cut(s) 67, 1921
EcoICRI GAGCTC 2 cut(s) 364, 2138
EcoNI CCTNNNNNAGG 1 cut(s) 898
EcoRI GAATTC 1 cut(s) 1172
EcoRII CCWGG 8 cut(s) 284, 586, 794, 876, 893, 1001, 1095, 1167
EcoT14I CCWWGG 1 cut(s) 2293
EcoT38I GRGCYC 2 cut(s) 366, 2140
ErhI CCWWGG 1 cut(s) 2293
Esp3I CGTCTC 2 cut(s) 1162, 2150
FaeI CATG 5 cut(s) 210, 283, 476, 1477, 2326
FaqI GGGAC 1 cut(s) 2024
FatI CATG 5 cut(s) 206, 279, 472, 1473, 2322
FblI GTMKAC 1 cut(s) 2345
Fnu4HI GCNGC 6 cut(s) 19, 22, 666, 1721, 1915, 2059
FokI GGATG 7 cut(s) 104, 812, 910, 1403, 1478, 1952, 2231
FriOI GRGCYC 2 cut(s) 366, 2140
Fsp4HI GCNGC 6 cut(s) 19, 22, 666, 1721, 1915, 2059
FspBI CTAG 8 cut(s) 930, 947, 1038, 1347, 1404, 1466, 1731, 2225
GlaI GCGC 2 cut(s) 633, 1828
GluI GCNGC 6 cut(s) 19, 22, 666, 1721, 1915, 2059
HaeII RGCGCY 2 cut(s) 635, 1830
HaeIII GGCC 5 cut(s) 524, 591, 1729, 2102, 2298
HapII CCGG 5 cut(s) 648, 1386, 1893, 2042, 2076
HhaI GCGC 2 cut(s) 634, 1829
Hin1I GRCGYC 1 cut(s) 1155
Hin1II CATG 5 cut(s) 210, 283, 476, 1477, 2326
Hin6I GCGC 2 cut(s) 632, 1827
HinP1I GCGC 2 cut(s) 632, 1827
HincII GTYRAC 1 cut(s) 1480
HindII GTYRAC 1 cut(s) 1480
HindIII AAGCTT 4 cut(s) 263, 1201, 1796, 1835
HpaII CCGG 5 cut(s) 648, 1386, 1893, 2042, 2076
HphI GGTGA 7 cut(s) 367, 859, 866, 1026, 1402, 1463, 1940
Hpy166II GTNNAC 5 cut(s) 177, 189, 490, 1480, 2346
Hpy188III TCNNGA 8 cut(s) 67, 280, 473, 1010, 1286, 1816, 2069, 2090
Hpy8I GTNNAC 5 cut(s) 177, 189, 490, 1480, 2346
HpyAV CCTTC 8 cut(s) 864, 1562, 1630, 1641, 1642, 1759, 1871, 2299
HpyCH4III ACNGT 6 cut(s) 470, 605, 1585, 1807, 2161, 2257
HpyCH4IV ACGT 4 cut(s) 77, 345, 1155, 1240
HpyF10VI GCNNNNNNNGC 5 cut(s) 530, 1422, 1726, 1839, 2319
HpySE526I ACGT 4 cut(s) 77, 345, 1155, 1240
Hsp92I GRCGYC 1 cut(s) 1155
Hsp92II CATG 5 cut(s) 210, 283, 476, 1477, 2326
HspAI GCGC 2 cut(s) 632, 1827
KpnI GGTACC 1 cut(s) 1029
Kzo9I GATC 9 cut(s) 226, 313, 772, 790, 850, 1382, 1707, 2086, 2260
LmnI GCTCC 4 cut(s) 361, 379, 2044, 2143
Lsp1109I GCAGC 6 cut(s) 30, 33, 652, 1732, 1926, 2070
MabI ACCWGGT 2 cut(s) 284, 876
MaeI CTAG 8 cut(s) 930, 947, 1038, 1347, 1404, 1466, 1731, 2225
MaeII ACGT 4 cut(s) 77, 345, 1155, 1240
MaeIII GTNAC 3 cut(s) 235, 1390, 1577
MalI GATC 9 cut(s) 228, 315, 774, 792, 852, 1384, 1709, 2088, 2262
MbiI CCGCTC 1 cut(s) 872
MboI GATC 9 cut(s) 226, 313, 772, 790, 850, 1382, 1707, 2086, 2260
MboII GAAGA 9 cut(s) 344, 350, 695, 745, 1204, 1594, 1717, 1746, 2038
MfeI CAATTG 2 cut(s) 1162, 1440
MflI RGATCY 4 cut(s) 313, 850, 1382, 1707
MhlI GDGCHC 5 cut(s) 179, 300, 366, 1458, 2140
MlsI TGGCCA 2 cut(s) 524, 591
MluNI TGGCCA 2 cut(s) 524, 591
MlyI GAGTC 3 cut(s) 1015, 1440, 1763
MmeI TCCRAC 3 cut(s) 2101, 2196, 2224
Mox20I TGGCCA 2 cut(s) 524, 591
MscI TGGCCA 2 cut(s) 524, 591
MseI TTAA 3 cut(s) 74, 1359, 2283
MslI CAYNNNNRTG 3 cut(s) 46, 303, 477
Msp20I TGGCCA 2 cut(s) 524, 591
MspA1I CMGCKG 3 cut(s) 711, 770, 1497
MspI CCGG 5 cut(s) 648, 1386, 1893, 2042, 2076
MunI CAATTG 2 cut(s) 1162, 1440
Mva1269I GAATGC 6 cut(s) 720, 843, 889, 1208, 2315, 2332
MvaI CCWGG 8 cut(s) 286, 588, 796, 878, 895, 1003, 1097, 1169
MwoI GCNNNNNNNGC 5 cut(s) 530, 1422, 1726, 1839, 2319
NciI CCSGG 4 cut(s) 649, 1387, 1894, 2042
NdeII GATC 9 cut(s) 226, 313, 772, 790, 850, 1382, 1707, 2086, 2260
NlaIII CATG 5 cut(s) 210, 283, 476, 1477, 2326
NlaIV GGNNCC 6 cut(s) 33, 299, 782, 1027, 1433, 1891
NmuCI GTSAC 2 cut(s) 235, 1390
NspI RCATGY 1 cut(s) 210
PaeI GCATGC 1 cut(s) 210
PaeR7I CTCGAG 2 cut(s) 67, 1921
PagI TCATGA 2 cut(s) 279, 472
PasI CCCWGGG 1 cut(s) 795
PceI AGGCCT 1 cut(s) 1729
PcsI WCGNNNNNNNCGW 2 cut(s) 74, 1455
PctI GAATGC 6 cut(s) 720, 843, 889, 1208, 2315, 2332
PfeI GAWTC 9 cut(s) 419, 451, 812, 829, 911, 1322, 1484, 2072, 2177
PflFI GACNNNGTC 2 cut(s) 739, 2036
PflMI CCANNNNNTGG 1 cut(s) 2109
PfoI TCCNGGA 3 cut(s) 893, 1001, 2040
PkrI GCNGC 6 cut(s) 20, 23, 667, 1722, 1916, 2060
PleI GAGTC 3 cut(s) 1014, 1440, 1763
PpsI GAGTC 3 cut(s) 1014, 1440, 1763
PshAI GACNNNNGTC 1 cut(s) 564
PsiI TTATAA 1 cut(s) 954
Psp124BI GAGCTC 2 cut(s) 366, 2140
Psp6I CCWGG 8 cut(s) 284, 586, 794, 876, 893, 1001, 1095, 1167
PspGI CCWGG 8 cut(s) 284, 586, 794, 876, 893, 1001, 1095, 1167
PspN4I GGNNCC 6 cut(s) 33, 299, 782, 1027, 1433, 1891
PspXI VCTCGAGB 1 cut(s) 1921
PsuI RGATCY 4 cut(s) 313, 850, 1382, 1707
PsyI GACNNNGTC 2 cut(s) 739, 2036
PvuII CAGCTG 1 cut(s) 770
RsaI GTAC 6 cut(s) 344, 642, 907, 1027, 1655, 1903
RsaNI GTAC 6 cut(s) 343, 641, 906, 1026, 1654, 1902
RseI CAYNNNNRTG 3 cut(s) 46, 303, 477
SacI GAGCTC 2 cut(s) 366, 2140
SaqAI TTAA 3 cut(s) 74, 1359, 2283
SatI GCNGC 6 cut(s) 19, 22, 666, 1721, 1915, 2059
Sau3AI GATC 9 cut(s) 226, 313, 772, 790, 850, 1382, 1707, 2086, 2260
ScaI AGTACT 1 cut(s) 907
SchI GAGTC 3 cut(s) 1015, 1440, 1763
SduI GDGCHC 5 cut(s) 179, 300, 366, 1458, 2140
SexAI ACCWGGT 2 cut(s) 284, 876
SfcI CTRYAG 1 cut(s) 2131
Sfr274I CTCGAG 2 cut(s) 67, 1921
SlaI CTCGAG 2 cut(s) 67, 1921
SmiMI CAYNNNNRTG 3 cut(s) 46, 303, 477
SmlI CTYRAG 6 cut(s) 67, 266, 317, 1822, 1921, 2090
SmoI CTYRAG 6 cut(s) 67, 266, 317, 1822, 1921, 2090
SphI GCATGC 1 cut(s) 210
SseBI AGGCCT 1 cut(s) 1729
SsiI CCGC 8 cut(s) 323, 711, 870, 1029, 1435, 1495, 1884, 2144
SspI AATATT 1 cut(s) 514
SspMI CTAG 8 cut(s) 930, 947, 1038, 1347, 1404, 1466, 1731, 2225
SstI GAGCTC 2 cut(s) 366, 2140
StuI AGGCCT 1 cut(s) 1729
StyI CCWWGG 1 cut(s) 2293
TaaI ACNGT 6 cut(s) 470, 605, 1585, 1807, 2161, 2257
TaiI ACGT 4 cut(s) 80, 348, 1158, 1243
TaqI TCGA 5 cut(s) 68, 229, 832, 1922, 2180
TatI WGTACW 1 cut(s) 905
TfiI GAWTC 9 cut(s) 419, 451, 812, 829, 911, 1322, 1484, 2072, 2177
Tru1I TTAA 3 cut(s) 74, 1359, 2283
Tru9I TTAA 3 cut(s) 74, 1359, 2283
TscAI CASTG 3 cut(s) 184, 1810, 1944
TseFI GTSAC 2 cut(s) 235, 1390
TseI GCWGC 6 cut(s) 18, 21, 665, 1720, 1914, 2058
Tsp45I GTSAC 2 cut(s) 235, 1390
TspDTI ATGAA 9 cut(s) 338, 345, 489, 1271, 1320, 1462, 2235, 2269, 2339
TspGWI ACGGA 2 cut(s) 609, 1133
TspRI CASTG 3 cut(s) 184, 1810, 1944
Tth111I GACNNNGTC 2 cut(s) 739, 2036
Van91I CCANNNNNTGG 1 cut(s) 2109
VneI GTGCAC 1 cut(s) 175
XagI CCTNNNNNAGG 1 cut(s) 898
XapI RAATTY 1 cut(s) 1172
XceI RCATGY 1 cut(s) 210
XcmI CCANNNNNNNNNTGG 2 cut(s) 128, 792
XhoI CTCGAG 2 cut(s) 67, 1921
XmiI GTMKAC 1 cut(s) 2345
XspI CTAG 8 cut(s) 930, 947, 1038, 1347, 1404, 1466, 1731, 2225
ZraI GACGTC 1 cut(s) 1156
ZrmI AGTACT 1 cut(s) 907
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.