MD04G1092500.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0008404
Physical Location & Seq
Reverse (-)
16467106 .. 16471184
4079 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1092500.v1.1.491

Sequence Viewer

Length: 729 bp
ATGGCTGAAACCTCTGCTGCTCTCTGCTTCTCACCCTTCACTTCCCTCTTCTCCTTCATTCGCGCCAAAGACTCCTCTTTCTCTTCATTCTCCACCGATTTCCCTCCAAAATCTCCAAATTCCCACCCTTGTCCCTCCCGCCTTCTCCGGTTTCCCGCCAAATCGTCCTCCGATTCGTCCAATTCCTTACCCGACGATCTCTTCGGCTTCTTTCCTTGGTCCAATGACTCTGAAAAAATTGAATGGGTTCCTGAAGAGAGAGTCACATTGTTCACTTCTGATGGACTAATTCAAATTGGAGGCTCCATGGTCCCTCGACGTCTCACTTCTTCGAATAAGAAGCAAGGTCAGTCAAAGACTAATCAAAGATTTCAGAGATTTAAAGAGAGTGATTACATGGACACAGAGCAGGGTTTGTGTCTGGGTGCTCTATTCGATATTGCAGCAACAAATGGCCTTGATACGGGCAGGAGACTTTGTATCTTTGGCTTTTGCCGTTCCATTGAGATGCTAAGCGATGTTGTGGAAGACACCGTTTTGGAACACGGTGGCGAGTGTGTTGTTGCTGCAGAGAAAGCCATGAAAGGTGGTTTGCAAGAGAAGCTAAGAATGACGGTTGCAGTGCCGTTACTTTGGGGGGTCCCTCCTGCCGCTGAAACACTTCACCTTGCCGTAAAGAGTGGCGGAGGAATTGTAGACAAAGTTTATTGGCAATGGGATTTCTTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

243

Amino Acids

26.71

Weight (kDa)

5.48

Isoelectric Point (pI)

51.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7811 PF25103 119 - 242 1.2e-63 Domain of unknown function (DUF7811)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 727
AatII GACGTC 1 cut(s) 322
AccI GTMKAC 1 cut(s) 696
AccII CGCG 1 cut(s) 63
AciI CCGC 4 cut(s) 139, 156, 651, 684
AcsI RAATTY 1 cut(s) 118
AcuI CTGAAG 1 cut(s) 273
AcyI GRCGYC 1 cut(s) 319
AfiI CCNNNNNNNGG 1 cut(s) 463
AgsI TTSAA 2 cut(s) 242, 293
AluBI AGCT 1 cut(s) 604
AluI AGCT 1 cut(s) 604
Alw21I GWGCWC 1 cut(s) 430
Alw26I GTCTC 2 cut(s) 326, 466
AoxI GGCC 1 cut(s) 454
ApeKI GCWGC 3 cut(s) 17, 443, 566
ApoI RAATTY 1 cut(s) 118
ArsI GACNNNNNNTTYG 2 cut(s) 185, 217
Asp700I GAANNNNTTC 2 cut(s) 246, 660
AspLEI GCGC 1 cut(s) 65
AspS9I GGNCC 3 cut(s) 219, 310, 640
AsuHPI GGTGA 2 cut(s) 24, 656
AsuII TTCGAA 1 cut(s) 332
AvaII GGWCC 3 cut(s) 219, 310, 640
BbsI GAAGAC 1 cut(s) 534
Bbv12I GWGCWC 1 cut(s) 430
BbvI GCAGC 3 cut(s) 4, 455, 553
BccI CCATC 1 cut(s) 275
BceAI ACGGC 3 cut(s) 480, 610, 656
BcoDI GTCTC 2 cut(s) 326, 466
BfmI CTRYAG 1 cut(s) 567
BisI GCNGC 4 cut(s) 18, 444, 567, 651
BlpI GCTNAGC 1 cut(s) 512
BlsI GCNGC 4 cut(s) 19, 445, 568, 652
Bme18I GGWCC 3 cut(s) 219, 310, 640
BmgT120I GGNCC 3 cut(s) 219, 310, 640
BmiI GGNNCC 5 cut(s) 249, 304, 312, 641, 642
BmsI GCATC 1 cut(s) 498
BpiI GAAGAC 1 cut(s) 534
Bpu1102I GCTNAGC 1 cut(s) 512
Bpu14I TTCGAA 1 cut(s) 332
BsaHI GRCGYC 1 cut(s) 319
BsaJI CCNNGG 2 cut(s) 215, 306
BsaWI WCCGGW 1 cut(s) 147
BsaXI ACNNNNNCTCC 4 cut(s) 463, 493, 678, 708
Bsc4I CCNNNNNNNGG 1 cut(s) 463
Bse3DI GCAATG 1 cut(s) 719
BseDI CCNNGG 2 cut(s) 215, 306
BseLI CCNNNNNNNGG 1 cut(s) 463
BseMI GCAATG 1 cut(s) 719
BseRI GAGGAG 1 cut(s) 64
BseXI GCAGC 3 cut(s) 4, 455, 553
Bsh1236I CGCG 1 cut(s) 63
BshFI GGCC 1 cut(s) 456
BsiHKAI GWGCWC 1 cut(s) 430
BsiSI CCGG 1 cut(s) 148
BslFI GGGAC 3 cut(s) 117, 296, 626
BslI CCNNNNNNNGG 1 cut(s) 463
BsmAI GTCTC 2 cut(s) 326, 466
BsmBI CGTCTC 1 cut(s) 326
BsmFI GGGAC 3 cut(s) 117, 296, 626
BsnI GGCC 1 cut(s) 456
Bsp119I TTCGAA 1 cut(s) 332
Bsp1286I GDGCHC 1 cut(s) 430
Bsp143I GATC 1 cut(s) 196
Bsp1720I GCTNAGC 1 cut(s) 512
Bsp19I CCATGG 1 cut(s) 306
BspACI CCGC 4 cut(s) 139, 156, 651, 684
BspANI GGCC 1 cut(s) 456
BspFNI CGCG 1 cut(s) 63
BspLI GGNNCC 5 cut(s) 249, 304, 312, 641, 642
BspMAI CTGCAG 1 cut(s) 571
BspT104I TTCGAA 1 cut(s) 332
BsrDI GCAATG 1 cut(s) 719
BssECI CCNNGG 2 cut(s) 215, 306
BssMI GATC 1 cut(s) 196
BssNI GRCGYC 1 cut(s) 319
BssT1I CCWWGG 2 cut(s) 215, 306
Bst4CI ACNGT 3 cut(s) 535, 548, 616
Bst6I CTCTTC 4 cut(s) 53, 88, 206, 249
BstACI GRCGYC 1 cut(s) 319
BstBI TTCGAA 1 cut(s) 332
BstDEI CTNAG 2 cut(s) 512, 605
BstDSI CCRYGG 1 cut(s) 306
BstFNI CGCG 1 cut(s) 63
BstHHI GCGC 1 cut(s) 65
BstKTI GATC 1 cut(s) 199
BstMAI GTCTC 2 cut(s) 326, 466
BstMBI GATC 1 cut(s) 196
BstMWI GCNNNNNNNGC 2 cut(s) 575, 601
BstSFI CTRYAG 1 cut(s) 567
BstUI CGCG 1 cut(s) 63
BstV1I GCAGC 3 cut(s) 4, 455, 553
BstV2I GAAGAC 1 cut(s) 534
BsuRI GGCC 1 cut(s) 456
BtgI CCRYGG 1 cut(s) 306
BtgZI GCGATG 1 cut(s) 531
BtsI GCAGTG 1 cut(s) 627
BtsIMutI CAGTG 1 cut(s) 627
CfoI GCGC 1 cut(s) 65
Cfr13I GGNCC 3 cut(s) 219, 310, 640
CviAII CATG 3 cut(s) 307, 397, 580
CviJI RGCY 7 cut(s) 5, 207, 303, 456, 489, 578, 604
CviKI_1 RGCY 7 cut(s) 5, 207, 303, 456, 489, 578, 604
DdeI CTNAG 2 cut(s) 512, 605
DpnI GATC 1 cut(s) 198
DpnII GATC 1 cut(s) 196
DraI TTTAAA 1 cut(s) 382
Eam1104I CTCTTC 4 cut(s) 53, 88, 206, 249
EarI CTCTTC 4 cut(s) 53, 88, 206, 249
EciI GGCGGA 1 cut(s) 699
Eco130I CCWWGG 2 cut(s) 215, 306
Eco47I GGWCC 3 cut(s) 219, 310, 640
Eco57I CTGAAG 1 cut(s) 273
EcoO109I RGGNCCY 1 cut(s) 640
EcoT14I CCWWGG 2 cut(s) 215, 306
ErhI CCWWGG 2 cut(s) 215, 306
Esp3I CGTCTC 1 cut(s) 326
FaeI CATG 3 cut(s) 310, 400, 583
FaiI YATR 4 cut(s) 308, 398, 581, 727
FaqI GGGAC 3 cut(s) 117, 296, 626
FatI CATG 3 cut(s) 306, 396, 579
FauI CCCGC 2 cut(s) 146, 163
FblI GTMKAC 1 cut(s) 696
Fnu4HI GCNGC 4 cut(s) 18, 444, 567, 651
Fsp4HI GCNGC 4 cut(s) 18, 444, 567, 651
GlaI GCGC 1 cut(s) 64
GluI GCNGC 4 cut(s) 18, 444, 567, 651
HaeIII GGCC 1 cut(s) 456
HapII CCGG 1 cut(s) 148
HhaI GCGC 1 cut(s) 65
Hin1I GRCGYC 1 cut(s) 319
Hin1II CATG 3 cut(s) 310, 400, 583
Hin6I GCGC 1 cut(s) 63
HinP1I GCGC 1 cut(s) 63
HinfI GANTC 4 cut(s) 71, 173, 227, 261
HpaII CCGG 1 cut(s) 148
HphI GGTGA 2 cut(s) 24, 656
Hpy166II GTNNAC 2 cut(s) 273, 697
Hpy188I TCNGA 4 cut(s) 172, 232, 280, 375
Hpy188III TCNNGA 1 cut(s) 251
Hpy8I GTNNAC 2 cut(s) 273, 697
Hpy99I CGWCG 2 cut(s) 197, 321
HpyAV CCTTC 3 cut(s) 46, 64, 152
HpyCH4III ACNGT 3 cut(s) 535, 548, 616
HpyCH4IV ACGT 1 cut(s) 319
HpyCH4V TGCA 4 cut(s) 443, 569, 595, 620
HpyF10VI GCNNNNNNNGC 2 cut(s) 575, 601
HpyF3I CTNAG 2 cut(s) 512, 605
HpySE526I ACGT 1 cut(s) 319
Hsp92I GRCGYC 1 cut(s) 319
Hsp92II CATG 3 cut(s) 310, 400, 583
HspAI GCGC 1 cut(s) 63
KflI GGGWCCC 1 cut(s) 640
Kzo9I GATC 1 cut(s) 196
LmnI GCTCC 1 cut(s) 308
LpnPI CCDG 6 cut(s) 161, 264, 395, 407, 454, 660
Lsp1109I GCAGC 3 cut(s) 4, 455, 553
LweI GCATC 1 cut(s) 498
MaeII ACGT 1 cut(s) 319
MaeIII GTNAC 2 cut(s) 262, 627
MalI GATC 1 cut(s) 198
MboI GATC 1 cut(s) 196
MboII GAAGA 6 cut(s) 40, 75, 193, 266, 321, 539
MhlI GDGCHC 1 cut(s) 430
MluCI AATT 6 cut(s) 118, 181, 237, 288, 294, 690
MlyI GAGTC 3 cut(s) 65, 221, 270
MroXI GAANNNNTTC 2 cut(s) 246, 660
MseI TTAA 1 cut(s) 381
MslI CAYNNNNRTG 1 cut(s) 506
MspA1I CMGCKG 1 cut(s) 653
MspI CCGG 1 cut(s) 148
MvnI CGCG 1 cut(s) 63
MwoI GCNNNNNNNGC 2 cut(s) 575, 601
NcoI CCATGG 1 cut(s) 306
NdeII GATC 1 cut(s) 196
NlaIII CATG 3 cut(s) 310, 400, 583
NlaIV GGNNCC 5 cut(s) 249, 304, 312, 641, 642
NmuCI GTSAC 1 cut(s) 262
NspV TTCGAA 1 cut(s) 332
PdmI GAANNNNTTC 2 cut(s) 246, 660
PfeI GAWTC 1 cut(s) 173
PkrI GCNGC 4 cut(s) 19, 445, 568, 652
PleI GAGTC 3 cut(s) 65, 221, 269
PpsI GAGTC 3 cut(s) 65, 221, 269
PpuMI RGGWCCY 1 cut(s) 640
PsiI TTATAA 1 cut(s) 727
Psp5II RGGWCCY 1 cut(s) 640
PspN4I GGNNCC 5 cut(s) 249, 304, 312, 641, 642
PspPI GGNCC 3 cut(s) 219, 310, 640
PspPPI RGGWCCY 1 cut(s) 640
PstI CTGCAG 1 cut(s) 571
RseI CAYNNNNRTG 1 cut(s) 506
SaqAI TTAA 1 cut(s) 381
SatI GCNGC 4 cut(s) 18, 444, 567, 651
Sau3AI GATC 1 cut(s) 196
Sau96I GGNCC 3 cut(s) 219, 310, 640
SchI GAGTC 3 cut(s) 65, 221, 270
SduI GDGCHC 1 cut(s) 430
SetI ASST 6 cut(s) 14, 322, 349, 589, 606, 669
SfaNI GCATC 1 cut(s) 498
SfcI CTRYAG 1 cut(s) 567
SfuI TTCGAA 1 cut(s) 332
SinI GGWCC 3 cut(s) 219, 310, 640
SmiMI CAYNNNNRTG 1 cut(s) 506
Sse9I AATT 6 cut(s) 118, 181, 237, 288, 294, 690
SsiI CCGC 4 cut(s) 139, 156, 651, 684
StyI CCWWGG 2 cut(s) 215, 306
TaaI ACNGT 3 cut(s) 535, 548, 616
TaiI ACGT 1 cut(s) 322
TaqI TCGA 3 cut(s) 316, 332, 435
TasI AATT 6 cut(s) 118, 181, 237, 288, 294, 690
TauI GCSGC 1 cut(s) 653
TfiI GAWTC 1 cut(s) 173
Tru1I TTAA 1 cut(s) 381
Tru9I TTAA 1 cut(s) 381
TscAI CASTG 1 cut(s) 627
TseFI GTSAC 1 cut(s) 262
TseI GCWGC 3 cut(s) 17, 443, 566
Tsp45I GTSAC 1 cut(s) 262
TspDTI ATGAA 3 cut(s) 46, 75, 596
TspRI CASTG 1 cut(s) 627
VpaK11BI GGWCC 3 cut(s) 219, 310, 640
XapI RAATTY 1 cut(s) 118
XmiI GTMKAC 1 cut(s) 696
XmnI GAANNNNTTC 2 cut(s) 246, 660
ZraI GACGTC 1 cut(s) 320
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.